BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00000769
         (508 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]      296   1e-78
gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudi...   294   6e-78
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P...   280   9e-74
ref|XP_002299097.1| cytochrome P450 [Populus trichocarpa] gi|222...   251   3e-65
ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   251   4e-65

>gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]
          Length = 476

 Score =  296 bits (758), Expect = 1e-78
 Identities = 138/169 (81%), Positives = 155/169 (91%)
 Frame = -2

Query: 507 GHDTSAVSITLVMKSLGEHPHVYEKVLREHLEICKTKEAGELLKWEDIQKMRYSWSVVSE 328
           GHDTSA+SITL+MK+LGEH  VY+KVL+E LEI K KEAGELLKWEDIQKM+YSWSVV E
Sbjct: 287 GHDTSALSITLLMKALGEHSDVYDKVLKEQLEISKGKEAGELLKWEDIQKMKYSWSVVCE 346

Query: 327 VMRLNPPVIGAFREALVDFEYAGYTIPKGWKLCWNAASTHKDQAHFDDVMRFDPSRFEGA 148
           VMRLNPPVIGA+REALVD +YAGYTIPKGWKL W+A ST +D+A+F+DV RFDPSRFEGA
Sbjct: 347 VMRLNPPVIGAYREALVDIDYAGYTIPKGWKLHWSAVSTQRDEANFEDVTRFDPSRFEGA 406

Query: 147 GPSPFTFVPFGGGPRMCLGKEFARVQVLVFLHNIVTKFKWELLIPDEKI 1
           GP+PFTFVPFGGGPRMCLGKEFAR++VL FLHNIV  FKW+LLIPDEKI
Sbjct: 407 GPTPFTFVPFGGGPRMCLGKEFARLEVLAFLHNIVINFKWDLLIPDEKI 455


>gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudiana]
           gi|189418962|gb|ACD93722.1| ent-kaurenoic acid
           13-hydroxylase [Stevia rebaudiana]
          Length = 476

 Score =  294 bits (752), Expect = 6e-78
 Identities = 136/169 (80%), Positives = 154/169 (91%)
 Frame = -2

Query: 507 GHDTSAVSITLVMKSLGEHPHVYEKVLREHLEICKTKEAGELLKWEDIQKMRYSWSVVSE 328
           GHDTSA+SITL+MK+LGEH  VY+KVL+E LEI KTKEA E LKWEDIQKM+YSWSV+ E
Sbjct: 287 GHDTSALSITLLMKTLGEHSDVYDKVLKEQLEISKTKEAWESLKWEDIQKMKYSWSVICE 346

Query: 327 VMRLNPPVIGAFREALVDFEYAGYTIPKGWKLCWNAASTHKDQAHFDDVMRFDPSRFEGA 148
           VMRLNPPVIG +REALVD +YAGYTIPKGWKL W+A ST +D+A+F+DV RFDPSRFEGA
Sbjct: 347 VMRLNPPVIGTYREALVDIDYAGYTIPKGWKLHWSAVSTQRDEANFEDVTRFDPSRFEGA 406

Query: 147 GPSPFTFVPFGGGPRMCLGKEFARVQVLVFLHNIVTKFKWELLIPDEKI 1
           GP+PFTFVPFGGGPRMCLGKEFAR++VL FLHNIVT FKW+LLIPDEKI
Sbjct: 407 GPTPFTFVPFGGGPRMCLGKEFARLEVLAFLHNIVTNFKWDLLIPDEKI 455


>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
           annua]
          Length = 480

 Score =  280 bits (716), Expect = 9e-74
 Identities = 128/169 (75%), Positives = 148/169 (87%)
 Frame = -2

Query: 507 GHDTSAVSITLVMKSLGEHPHVYEKVLREHLEICKTKEAGELLKWEDIQKMRYSWSVVSE 328
           GHDTSAVSITL+MKSL EHP VY+ VL+E L I + K  GE+L WEDIQKMRYSW VV E
Sbjct: 291 GHDTSAVSITLLMKSLAEHPQVYDNVLKEQLGILEAKAPGEMLNWEDIQKMRYSWYVVCE 350

Query: 327 VMRLNPPVIGAFREALVDFEYAGYTIPKGWKLCWNAASTHKDQAHFDDVMRFDPSRFEGA 148
           VMRL PPV+G+FREALVDFEYAGYTIPKGWK+ W+A  THK++ +F +  +FDPSRFEGA
Sbjct: 351 VMRLIPPVVGSFREALVDFEYAGYTIPKGWKIIWSAVMTHKEENNFPNATKFDPSRFEGA 410

Query: 147 GPSPFTFVPFGGGPRMCLGKEFARVQVLVFLHNIVTKFKWELLIPDEKI 1
           GP+PFT+VPFGGGPRMCLGKE ARV++LVFLHNI+TKFKW+LLIPDEKI
Sbjct: 411 GPTPFTYVPFGGGPRMCLGKELARVRILVFLHNIMTKFKWDLLIPDEKI 459


>ref|XP_002299097.1| cytochrome P450 [Populus trichocarpa] gi|222846355|gb|EEE83902.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 483

 Score =  251 bits (642), Expect = 3e-65
 Identities = 114/168 (67%), Positives = 139/168 (82%)
 Frame = -2

Query: 504 HDTSAVSITLVMKSLGEHPHVYEKVLREHLEICKTKEAGELLKWEDIQKMRYSWSVVSEV 325
           H+T+  ++T V+K L E P VYE VLRE L+I K+KEAGELLKWEDIQKM+YSW VVSEV
Sbjct: 293 HETTTSAMTCVIKYLAEMPEVYEMVLREQLDIAKSKEAGELLKWEDIQKMKYSWRVVSEV 352

Query: 324 MRLNPPVIGAFREALVDFEYAGYTIPKGWKLCWNAASTHKDQAHFDDVMRFDPSRFEGAG 145
           +R+ PP+ G FR+A+VDF YAGYTIPKGWKL W+  +T KD AHF +   FDPSR+EGAG
Sbjct: 353 LRMIPPISGTFRQAIVDFTYAGYTIPKGWKLYWSPNTTTKDPAHFPNAEDFDPSRYEGAG 412

Query: 144 PSPFTFVPFGGGPRMCLGKEFARVQVLVFLHNIVTKFKWELLIPDEKI 1
           P+P+T VPFGGGPRMCLG E+ R ++LVFLHNIV +FKW+LLIPDEK+
Sbjct: 413 PAPYTHVPFGGGPRMCLGYEYVRPKILVFLHNIVKRFKWDLLIPDEKV 460


>ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 716B2-like [Vitis
           vinifera]
          Length = 473

 Score =  251 bits (641), Expect = 4e-65
 Identities = 116/169 (68%), Positives = 139/169 (82%)
 Frame = -2

Query: 507 GHDTSAVSITLVMKSLGEHPHVYEKVLREHLEICKTKEAGELLKWEDIQKMRYSWSVVSE 328
           GHDT+A +ITL++K LGE P VY KVLRE ++I ++KE GELL+W+D QKMRYSW V SE
Sbjct: 286 GHDTTASAITLLIKYLGELPEVYAKVLREKIDITESKEPGELLQWKDXQKMRYSWHVASE 345

Query: 327 VMRLNPPVIGAFREALVDFEYAGYTIPKGWKLCWNAASTHKDQAHFDDVMRFDPSRFEGA 148
           VMRL+PP  G+FREALVDF YAGY IPKGWKL W   ST +D A F +   FD SRFEGA
Sbjct: 346 VMRLSPPASGSFREALVDFSYAGYNIPKGWKLYWGTGSTQRDPAFFRNPDNFDASRFEGA 405

Query: 147 GPSPFTFVPFGGGPRMCLGKEFARVQVLVFLHNIVTKFKWELLIPDEKI 1
           GP+PF++VPFGGGPRMCLG+EFAR+Q+LVF+HNIV +F W+LL PDEKI
Sbjct: 406 GPAPFSYVPFGGGPRMCLGQEFARLQILVFMHNIVKRFTWDLLNPDEKI 454