BLASTX nr result
ID: Atractylodes22_contig00000768
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes22_contig00000768
(1272 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudi... 514 e-143
gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana] 513 e-143
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P... 469 e-130
ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P... 419 e-115
ref|XP_002299098.1| cytochrome P450 [Populus trichocarpa] gi|222... 398 e-108
>gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudiana]
gi|189418962|gb|ACD93722.1| ent-kaurenoic acid
13-hydroxylase [Stevia rebaudiana]
Length = 476
Score = 514 bits (1324), Expect = e-143
Identities = 249/356 (69%), Positives = 292/356 (82%), Gaps = 1/356 (0%)
Frame = +3
Query: 3 DEAKWMRKMMLAHLGVGPD-LATHYSSTMDSVTRRHIQIHWQGKEELNVYKSVKLYTFEL 179
DEAKWMRKM+L++LG PD ATHY+ TMD VTRRHI +HW+GKEE+NV+++VKLY FEL
Sbjct: 123 DEAKWMRKMLLSYLG--PDAFATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFEL 180
Query: 180 ACRLFMSLEEPNQIAKLSSHFKIFLKGIIQLPLDFPGTRFYSAKKAAAAIRTQLMPIIKE 359
ACRLFM+L++PN IAKL S F IFLKGII+LP+D PGTRFYS+KKAAAAIR +L +IK
Sbjct: 181 ACRLFMNLDDPNHIAKLGSLFNIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKA 240
Query: 360 KRMALEEGKXXXXXXXXXXXXXXXXENGKYLTEMEIANTILLLLFAGHDTSTAVITLLMK 539
+++ L+EGK ENG +LTE EI + ILLLLFAGHDTS ITLLMK
Sbjct: 241 RKLELKEGKASSSQDLLSHLLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMK 300
Query: 540 SLSEHPHVYDKVLRENLEICKTKEVGELLKWEDIQKMKYSWCVISEVMRLYPTIIGPFRE 719
+L EH VYDKVL+E LEI KTKE E LKWEDIQKMKYSW VI EVMRL P +IG +RE
Sbjct: 301 TLGEHSDVYDKVLKEQLEISKTKEAWESLKWEDIQKMKYSWSVICEVMRLNPPVIGTYRE 360
Query: 720 ALVDIEYAGYTIPKGWKLYGPVPSTHKDEAHYKDVTRFDPSRFEGAGPSPYTYVPFGGGP 899
ALVDI+YAGYTIPKGWKL+ ST +DEA+++DVTRFDPSRFEGAGP+P+T+VPFGGGP
Sbjct: 361 ALVDIDYAGYTIPKGWKLHWSAVSTQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGP 420
Query: 900 RMCMGKEYARVEILVFLHNIVTKFKWEMLIPDEKIVYDPMVTPEKGLPIRLHPHQV 1067
RMC+GKE+AR+E+L FLHNIVT FKW++LIPDEKI YDPM TP KGLPIRLHPHQV
Sbjct: 421 RMCLGKEFARLEVLAFLHNIVTNFKWDLLIPDEKIEYDPMATPAKGLPIRLHPHQV 476
>gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]
Length = 476
Score = 513 bits (1320), Expect = e-143
Identities = 247/356 (69%), Positives = 292/356 (82%), Gaps = 1/356 (0%)
Frame = +3
Query: 3 DEAKWMRKMMLAHLGVGPD-LATHYSSTMDSVTRRHIQIHWQGKEELNVYKSVKLYTFEL 179
DEAKWMRKM+L++LG PD ATHY+ TMD VTRRHI +HW+GKEE+NV+++VKLY FEL
Sbjct: 123 DEAKWMRKMLLSYLG--PDAFATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFEL 180
Query: 180 ACRLFMSLEEPNQIAKLSSHFKIFLKGIIQLPLDFPGTRFYSAKKAAAAIRTQLMPIIKE 359
ACRLFM+L++PN IAKL S F IFLKGII+LP+D PGTRFYS+KKAAAAIR +L +IK
Sbjct: 181 ACRLFMNLDDPNHIAKLGSLFNIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKA 240
Query: 360 KRMALEEGKXXXXXXXXXXXXXXXXENGKYLTEMEIANTILLLLFAGHDTSTAVITLLMK 539
+++ L+EGK ENG +LTE EI + ILLLLFAGHDTS ITLLMK
Sbjct: 241 RKLELKEGKPSSSQDLLSHLLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMK 300
Query: 540 SLSEHPHVYDKVLRENLEICKTKEVGELLKWEDIQKMKYSWCVISEVMRLYPTIIGPFRE 719
+L EH VYDKVL+E LEI K KE GELLKWEDIQKMKYSW V+ EVMRL P +IG +RE
Sbjct: 301 ALGEHSDVYDKVLKEQLEISKGKEAGELLKWEDIQKMKYSWSVVCEVMRLNPPVIGAYRE 360
Query: 720 ALVDIEYAGYTIPKGWKLYGPVPSTHKDEAHYKDVTRFDPSRFEGAGPSPYTYVPFGGGP 899
ALVDI+YAGYTIPKGWKL+ ST +DEA+++DVTRFDPSRFEGAGP+P+T+VPFGGGP
Sbjct: 361 ALVDIDYAGYTIPKGWKLHWSAVSTQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGP 420
Query: 900 RMCMGKEYARVEILVFLHNIVTKFKWEMLIPDEKIVYDPMVTPEKGLPIRLHPHQV 1067
RMC+GKE+AR+E+L FLHNIV FKW++LIPDEKI YDP+ TP KGLPIRLHPHQV
Sbjct: 421 RMCLGKEFARLEVLAFLHNIVINFKWDLLIPDEKIEYDPIATPAKGLPIRLHPHQV 476
>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
annua]
Length = 480
Score = 469 bits (1208), Expect = e-130
Identities = 226/357 (63%), Positives = 278/357 (77%), Gaps = 2/357 (0%)
Frame = +3
Query: 3 DEAKWMRKMMLAHLGVGPD-LATHYSSTMDSVTRRHIQIHWQGKEELNVYKSVKLYTFEL 179
DEAKW+RKMM A+LG PD L+ Y+ TM+ VTR HIQ HWQGK EL V+++V+ Y FEL
Sbjct: 126 DEAKWLRKMMFAYLG--PDALSNRYTGTMEVVTRLHIQNHWQGKSELKVFETVRPYLFEL 183
Query: 180 ACRLFMSLEEPNQIAKLSSHFKIFLKGIIQLPLDFPGTRFYSAKKAAAAIRTQLMPIIKE 359
ACRLF+SL++P +A+L + F FLKG+ +LP++ PGTRFY AK+AA AI+ QL+ IIK+
Sbjct: 184 ACRLFLSLDDPKHVAELGTLFNTFLKGLTELPINIPGTRFYRAKRAANAIKKQLIVIIKQ 243
Query: 360 KRMAL-EEGKXXXXXXXXXXXXXXXXENGKYLTEMEIANTILLLLFAGHDTSTAVITLLM 536
+R AL +E + ENG++L+E EIAN +LLLLFAGHDTS ITLLM
Sbjct: 244 RRQALKQEDQSSSFEDLLSHLLVSSDENGRFLSEAEIANNVLLLLFAGHDTSAVSITLLM 303
Query: 537 KSLSEHPHVYDKVLRENLEICKTKEVGELLKWEDIQKMKYSWCVISEVMRLYPTIIGPFR 716
KSL+EHP VYD VL+E L I + K GE+L WEDIQKM+YSW V+ EVMRL P ++G FR
Sbjct: 304 KSLAEHPQVYDNVLKEQLGILEAKAPGEMLNWEDIQKMRYSWYVVCEVMRLIPPVVGSFR 363
Query: 717 EALVDIEYAGYTIPKGWKLYGPVPSTHKDEAHYKDVTRFDPSRFEGAGPSPYTYVPFGGG 896
EALVD EYAGYTIPKGWK+ THK+E ++ + T+FDPSRFEGAGP+P+TYVPFGGG
Sbjct: 364 EALVDFEYAGYTIPKGWKIIWSAVMTHKEENNFPNATKFDPSRFEGAGPTPFTYVPFGGG 423
Query: 897 PRMCMGKEYARVEILVFLHNIVTKFKWEMLIPDEKIVYDPMVTPEKGLPIRLHPHQV 1067
PRMC+GKE ARV ILVFLHNI+TKFKW++LIPDEKI YDP+ TP KGLP+RLHPHQV
Sbjct: 424 PRMCLGKELARVRILVFLHNIMTKFKWDLLIPDEKIGYDPLATPVKGLPVRLHPHQV 480
>ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 716B2-like [Vitis
vinifera]
Length = 473
Score = 419 bits (1078), Expect = e-115
Identities = 204/353 (57%), Positives = 258/353 (73%)
Frame = +3
Query: 3 DEAKWMRKMMLAHLGVGPDLATHYSSTMDSVTRRHIQIHWQGKEELNVYKSVKLYTFELA 182
DEAK MR+M+L L PD Y TMD VT+ HI+ W+G EE+ VY +KLYTFELA
Sbjct: 123 DEAKRMRRMLLTFLN--PDALKRYVETMDLVTQHHIRTQWEGNEEMQVYPIIKLYTFELA 180
Query: 183 CRLFMSLEEPNQIAKLSSHFKIFLKGIIQLPLDFPGTRFYSAKKAAAAIRTQLMPIIKEK 362
CRLF S+++P I+KL++HF +FLKGII P++FPGT+F A KAA AIR +L + E+
Sbjct: 181 CRLFASMDDPGHISKLAAHFNVFLKGIIDFPINFPGTKFXYATKAANAIREELRVMPMER 240
Query: 363 RMALEEGKXXXXXXXXXXXXXXXXENGKYLTEMEIANTILLLLFAGHDTSTAVITLLMKS 542
R+ALEE +G++LTEMEI + ILLLLFAGHDT+ + ITLL+K
Sbjct: 241 RVALEEKTASATQDLLSHLLVTADASGRFLTEMEIIDNILLLLFAGHDTTASAITLLIKY 300
Query: 543 LSEHPHVYDKVLRENLEICKTKEVGELLKWEDIQKMKYSWCVISEVMRLYPTIIGPFREA 722
L E P VY KVLRE ++I ++KE GELL+W+D QKM+YSW V SEVMRL P G FREA
Sbjct: 301 LGELPEVYAKVLREKIDITESKEPGELLQWKDXQKMRYSWHVASEVMRLSPPASGSFREA 360
Query: 723 LVDIEYAGYTIPKGWKLYGPVPSTHKDEAHYKDVTRFDPSRFEGAGPSPYTYVPFGGGPR 902
LVD YAGY IPKGWKLY ST +D A +++ FD SRFEGAGP+P++YVPFGGGPR
Sbjct: 361 LVDFSYAGYNIPKGWKLYWGTGSTQRDPAFFRNPDNFDASRFEGAGPAPFSYVPFGGGPR 420
Query: 903 MCMGKEYARVEILVFLHNIVTKFKWEMLIPDEKIVYDPMVTPEKGLPIRLHPH 1061
MC+G+E+AR++ILVF+HNIV +F W++L PDEKI + PM+ P +GLPIRLHPH
Sbjct: 421 MCLGQEFARLQILVFMHNIVKRFTWDLLNPDEKIEHSPMLAPLEGLPIRLHPH 473
>ref|XP_002299098.1| cytochrome P450 [Populus trichocarpa] gi|222846356|gb|EEE83903.1|
cytochrome P450 [Populus trichocarpa]
Length = 414
Score = 398 bits (1023), Expect = e-108
Identities = 193/354 (54%), Positives = 251/354 (70%)
Frame = +3
Query: 3 DEAKWMRKMMLAHLGVGPDLATHYSSTMDSVTRRHIQIHWQGKEELNVYKSVKLYTFELA 182
D+AK MRKM+L L D Y MD V + HI+ HW+GKEEL ++ ++ LYTFEL+
Sbjct: 60 DDAKRMRKMLLTSLD--RDALKRYIDRMDLVAQNHIRTHWEGKEELKLHPTINLYTFELS 117
Query: 183 CRLFMSLEEPNQIAKLSSHFKIFLKGIIQLPLDFPGTRFYSAKKAAAAIRTQLMPIIKEK 362
CRLF S+++P I+KL+ HF IFLKG+I P+ PGT FY A KAA AI+ +L I + +
Sbjct: 118 CRLFASIDDPTHISKLAHHFDIFLKGVIHFPIYVPGTPFYRASKAADAIKEELRLIARRR 177
Query: 363 RMALEEGKXXXXXXXXXXXXXXXXENGKYLTEMEIANTILLLLFAGHDTSTAVITLLMKS 542
R AL++ +GK L+E EI + +L+LLFA HDT+T+ +T +MK
Sbjct: 178 REALDKKMESHRKDLLSHLLVTTDASGKLLSESEIVDNMLMLLFASHDTTTSAMTCVMKY 237
Query: 543 LSEHPHVYDKVLRENLEICKTKEVGELLKWEDIQKMKYSWCVISEVMRLYPTIIGPFREA 722
L+E P VY+ VLRE L+I K+KE GELLKWEDIQ+M+YS V+SEV+R+ P I G FR+A
Sbjct: 238 LAELPEVYEMVLREQLDIAKSKEAGELLKWEDIQRMRYSCNVVSEVLRMIPPIRGTFRKA 297
Query: 723 LVDIEYAGYTIPKGWKLYGPVPSTHKDEAHYKDVTRFDPSRFEGAGPSPYTYVPFGGGPR 902
LVD YAGY IPKGWKLY ST KD A++ + FDPSR+EGAGP+PYT+VPFGGG R
Sbjct: 298 LVDFTYAGYKIPKGWKLYWSPDSTTKDPAYFPNPEEFDPSRYEGAGPAPYTFVPFGGGGR 357
Query: 903 MCMGKEYARVEILVFLHNIVTKFKWEMLIPDEKIVYDPMVTPEKGLPIRLHPHQ 1064
+C+G EY+R +ILVF+HNIV +FKW++LIPDEK+ YDPM P GLPIR+ HQ
Sbjct: 358 VCIGNEYSRPQILVFMHNIVKRFKWDLLIPDEKVTYDPMPAPSHGLPIRIQQHQ 411