BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00000217
         (1193 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003610873.1| Diphthamide biosynthesis protein [Medicago t...   545   e-153
ref|XP_002325444.1| predicted protein [Populus trichocarpa] gi|2...   543   e-152
ref|XP_002265458.1| PREDICTED: diphthamide biosynthesis protein ...   535   e-149
ref|XP_004136856.1| PREDICTED: diphthamide biosynthesis protein ...   533   e-149
ref|XP_002529006.1| Diphthamide biosynthesis protein, putative [...   526   e-147

>ref|XP_003610873.1| Diphthamide biosynthesis protein [Medicago truncatula]
            gi|355512208|gb|AES93831.1| Diphthamide biosynthesis
            protein [Medicago truncatula]
          Length = 575

 Score =  545 bits (1404), Expect = e-153
 Identities = 264/375 (70%), Positives = 308/375 (82%), Gaps = 1/375 (0%)
 Frame = +3

Query: 66   PPPPTNATTHEEIRRPPPRQPKRFIKNQIPDSITNDPALNAAIALLPSNYNFEIHKSIWR 245
            P  P N  T     +P   +PKRFIKNQIPDSI NDP LNAAI++LPSNY+FE+HK +WR
Sbjct: 7    PEKPNNTITVSVKEKP---KPKRFIKNQIPDSILNDPLLNAAISILPSNYSFEVHKCVWR 63

Query: 246  LRSTAAKRVALQFPEGLLMYSLVISDIITAFTDVDRCFVLGDVTYGACCVXXXXXXXXXX 425
            +RSTAAKR+ALQFPEGLLMYSL++SDI T F  V  C+VLGDVTYGACC+          
Sbjct: 64   VRSTAAKRIALQFPEGLLMYSLILSDIFTTFAGVTHCYVLGDVTYGACCIDDFSAIALGA 123

Query: 426  XXXIHYGHSCLVPVDNTVIPCLYVFVDIKIDVERLINTVKLNF-DASITTKQLILAGTIQ 602
               IHYGHSCLVP+D+T IPCLYVFVDIKIDV+ L++TVKLN  D     K++ILAGTIQ
Sbjct: 124  EILIHYGHSCLVPIDSTKIPCLYVFVDIKIDVDHLVDTVKLNSKDLGFMGKRVILAGTIQ 183

Query: 603  FATAIRAAKSELENAGFRVMIPQSKPLSAGEVLGCTAPTIPMAQFNESKVVLVFVADGRF 782
            FA+ IRA K ELE  GFRV++PQSKPLSAGE+LGCTAP +     ++ + VLVFV+DGRF
Sbjct: 184  FASGIRAVKPELEKLGFRVLVPQSKPLSAGEILGCTAPKVGKELDDKEESVLVFVSDGRF 243

Query: 783  HLEAFMIANPRIKAFRYDPYIGSLFLEEYDHKGMKECRKNAILKSRDAKNWGIVLGTLGR 962
            HLEAFMIANP +KAFRYDPY+G LFLEEYDH GMK  RKNAILK+++A+NWG+VLGTLGR
Sbjct: 244  HLEAFMIANPGVKAFRYDPYVGKLFLEEYDHVGMKGSRKNAILKAKEARNWGVVLGTLGR 303

Query: 963  QGNPRILDRLEKKMGEKGLSWTIVLMSELSPVRIALFGDSIDAWIQIACPRLSIDWGDAF 1142
            QGNP+IL+RLE KMG+KG  +T+VLMSELSP RI+LF DS+DAWIQIACPRLSIDWGDAF
Sbjct: 304  QGNPKILERLEMKMGDKGFDYTVVLMSELSPYRISLFEDSVDAWIQIACPRLSIDWGDAF 363

Query: 1143 VKPLLTPFEAEIALG 1187
            VKP+LTPFEAEIALG
Sbjct: 364  VKPVLTPFEAEIALG 378


>ref|XP_002325444.1| predicted protein [Populus trichocarpa] gi|222862319|gb|EEE99825.1|
            predicted protein [Populus trichocarpa]
          Length = 428

 Score =  543 bits (1398), Expect = e-152
 Identities = 268/361 (74%), Positives = 302/361 (83%), Gaps = 5/361 (1%)
 Frame = +3

Query: 126  PKRFIKNQIPDSITNDPALNAAIALLPSNYNFEIHKSIWRLRSTAAKRVALQFPEGLLMY 305
            PKRFIK+QIPDSI NDP+LNA+I+LLPSNYNFEIHK IWR+RST AKR+ALQ PEGLLMY
Sbjct: 14   PKRFIKSQIPDSILNDPSLNASISLLPSNYNFEIHKCIWRVRSTNAKRIALQLPEGLLMY 73

Query: 306  SLVISDIITAFTDVDRCFVLGDVTYGACCVXXXXXXXXXXXXXIHYGHSCLVPVDNTVIP 485
            SL++SDI TAF DV  CFVLGDVTYGACCV             IHYGHSCL+P+D T IP
Sbjct: 74   SLILSDIFTAFADVTHCFVLGDVTYGACCVDDLSALALDADLLIHYGHSCLIPIDATKIP 133

Query: 486  CLYVFVDIKIDVERLINTVKLNFDASITTKQLILAGTIQFATAIRAAKSELENAGFRVMI 665
            CLYVFV+IKIDV RLINT+ LNF      + ++LAGTIQFA+AIR AK ELEN GF V+I
Sbjct: 134  CLYVFVEIKIDVSRLINTISLNFGTH--KRNIVLAGTIQFASAIREAKPELENNGFVVLI 191

Query: 666  PQSKPLSAGEVLGCTAP-----TIPMAQFNESKVVLVFVADGRFHLEAFMIANPRIKAFR 830
            PQSKPLSAGEVLGCTAP      +    F+E ++VLVFVADGRFHLEAFMIANP IKAFR
Sbjct: 192  PQSKPLSAGEVLGCTAPKMSKNVVGNLGFSEEEMVLVFVADGRFHLEAFMIANPGIKAFR 251

Query: 831  YDPYIGSLFLEEYDHKGMKECRKNAILKSRDAKNWGIVLGTLGRQGNPRILDRLEKKMGE 1010
            YDPY+G LFLEEYDHKGMKE RK AI +++ A++WG+VLGTLGRQGNPRIL+RLEKKMGE
Sbjct: 252  YDPYLGKLFLEEYDHKGMKEVRKKAIERAKGARSWGVVLGTLGRQGNPRILERLEKKMGE 311

Query: 1011 KGLSWTIVLMSELSPVRIALFGDSIDAWIQIACPRLSIDWGDAFVKPLLTPFEAEIALGD 1190
            KG  + +VLMSE+SP RIALF DS+DAWIQIACPRLSIDWGDAF KPLLTPFEAEIALGD
Sbjct: 312  KGFDYMVVLMSEISPGRIALFEDSVDAWIQIACPRLSIDWGDAFKKPLLTPFEAEIALGD 371

Query: 1191 I 1193
            +
Sbjct: 372  L 372


>ref|XP_002265458.1| PREDICTED: diphthamide biosynthesis protein 1-like [Vitis vinifera]
          Length = 461

 Score =  535 bits (1377), Expect = e-149
 Identities = 264/356 (74%), Positives = 293/356 (82%)
 Frame = +3

Query: 126  PKRFIKNQIPDSITNDPALNAAIALLPSNYNFEIHKSIWRLRSTAAKRVALQFPEGLLMY 305
            PKRF+KNQIPDSI N+ ALNAAIALLPSNYNFE+HK +WR+ S+ AKRVALQFPEGLLMY
Sbjct: 30   PKRFVKNQIPDSIINNAALNAAIALLPSNYNFEVHKCVWRILSSRAKRVALQFPEGLLMY 89

Query: 306  SLVISDIITAFTDVDRCFVLGDVTYGACCVXXXXXXXXXXXXXIHYGHSCLVPVDNTVIP 485
            SL +SDI T++   D C VLGDVTYGACCV             IHYGHSCLVP+D T IP
Sbjct: 90   SLHLSDIFTSYAGADHCLVLGDVTYGACCVDDLSAAALEADLLIHYGHSCLVPIDATSIP 149

Query: 486  CLYVFVDIKIDVERLINTVKLNFDASITTKQLILAGTIQFATAIRAAKSELENAGFRVMI 665
            CLYVFV+I IDV+ LI TVKLN   +    Q++LAGTIQF++AIRAAK ELE AGFRV+I
Sbjct: 150  CLYVFVEIAIDVDLLIGTVKLNLPPA---GQIVLAGTIQFSSAIRAAKPELEKAGFRVLI 206

Query: 666  PQSKPLSAGEVLGCTAPTIPMAQFNESKVVLVFVADGRFHLEAFMIANPRIKAFRYDPYI 845
            PQSKPLSAGEVLGCTAP I    F    VV VFVADGRFHLEAFMIANP IKA+RYDPYI
Sbjct: 207  PQSKPLSAGEVLGCTAPKISKTSFISDDVVAVFVADGRFHLEAFMIANPAIKAYRYDPYI 266

Query: 846  GSLFLEEYDHKGMKECRKNAILKSRDAKNWGIVLGTLGRQGNPRILDRLEKKMGEKGLSW 1025
            G L LEEYDHKGMK+ RKNAILK+R+ KNWGIVLGTLGRQGNPRIL+ L+ +M EKG SW
Sbjct: 267  GRLLLEEYDHKGMKDSRKNAILKAREGKNWGIVLGTLGRQGNPRILNMLQDRMREKGFSW 326

Query: 1026 TIVLMSELSPVRIALFGDSIDAWIQIACPRLSIDWGDAFVKPLLTPFEAEIALGDI 1193
            T+VLMSE+SP R+ LFGDS+DAWIQIACPRLSIDWGDAF KPLL PFEAEIALG I
Sbjct: 327  TVVLMSEISPARVLLFGDSVDAWIQIACPRLSIDWGDAFGKPLLNPFEAEIALGFI 382


>ref|XP_004136856.1| PREDICTED: diphthamide biosynthesis protein 1-like [Cucumis sativus]
            gi|449478949|ref|XP_004155462.1| PREDICTED: diphthamide
            biosynthesis protein 1-like [Cucumis sativus]
          Length = 462

 Score =  533 bits (1373), Expect = e-149
 Identities = 259/364 (71%), Positives = 299/364 (82%), Gaps = 4/364 (1%)
 Frame = +3

Query: 108  RPPPRQ---PKRFIKNQIPDSITNDPALNAAIALLPSNYNFEIHKSIWRLRSTAAKRVAL 278
            +PPPR    PKRF+KNQIPDSI NDPALNAAI+LLPSNYNFE+HK +WR+ S++A RVAL
Sbjct: 22   KPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNYNFEVHKCVWRILSSSASRVAL 81

Query: 279  QFPEGLLMYSLVISDIITAFTDVDRCFVLGDVTYGACCVXXXXXXXXXXXXXIHYGHSCL 458
            Q PEGLLMYSL++SDI+T F     CFVLGDVT+GACCV             +HYGHSCL
Sbjct: 82   QLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCVDDLSASALHADLLVHYGHSCL 141

Query: 459  VPVDNTVIPCLYVFVDIKIDVERLINTVKLNFDASITTKQLILAGTIQFATAIRAAKSEL 638
            VP+D+T IPCLYVFVDI IDV RL+NTV+LN D   +T  L+LAGTIQFA+AIRA+K EL
Sbjct: 142  VPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN-LVLAGTIQFASAIRASKMEL 200

Query: 639  ENAGFRVMIPQSKPLSAGEVLGCTAPTIPMAQF-NESKVVLVFVADGRFHLEAFMIANPR 815
            E  G RV+IPQS+PLSAGEVLGCTAP +  A F N  + V +FVADGRFHLEAFMIANP 
Sbjct: 201  ERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETVAIFVADGRFHLEAFMIANPG 260

Query: 816  IKAFRYDPYIGSLFLEEYDHKGMKECRKNAILKSRDAKNWGIVLGTLGRQGNPRILDRLE 995
            I+ FRYDPYIG L LEEYDH+GMK  RK AILK+++A NWGIVLGTLGRQGNP+IL+RLE
Sbjct: 261  IRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNWGIVLGTLGRQGNPKILERLE 320

Query: 996  KKMGEKGLSWTIVLMSELSPVRIALFGDSIDAWIQIACPRLSIDWGDAFVKPLLTPFEAE 1175
            +KM  KG  +T+VLMSE+SP R+ALF DS+DAWIQIACPRLSIDWGDAF KPLLTPFEAE
Sbjct: 321  EKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPRLSIDWGDAFAKPLLTPFEAE 380

Query: 1176 IALG 1187
            +ALG
Sbjct: 381  VALG 384


>ref|XP_002529006.1| Diphthamide biosynthesis protein, putative [Ricinus communis]
            gi|223531546|gb|EEF33376.1| Diphthamide biosynthesis
            protein, putative [Ricinus communis]
          Length = 434

 Score =  526 bits (1355), Expect = e-147
 Identities = 263/364 (72%), Positives = 293/364 (80%), Gaps = 2/364 (0%)
 Frame = +3

Query: 108  RPPPRQPKRFIKNQIPDSITNDPALNAAIALLPSNYNFEIHKSIWRLRSTAAKRVALQFP 287
            +P    PKRF+KNQIPDSI ND +LNAAIALLPSNYN EIHK +WR+RST  KR+ALQ P
Sbjct: 11   KPAKPPPKRFVKNQIPDSILNDASLNAAIALLPSNYNLEIHKCVWRIRSTGTKRLALQLP 70

Query: 288  EGLLMYSLVISDIITAFTDVDRCFVLGDVTYGACCVXXXXXXXXXXXXXIHYGHSCLVPV 467
            EGLLMYSL++SDI TAF  V  CF+LGDVTYGACC+             IHYGHSCLVP+
Sbjct: 71   EGLLMYSLILSDIFTAFAGVTHCFILGDVTYGACCIDDLSALALGADLLIHYGHSCLVPI 130

Query: 468  DNTVIPCLYVFVDIKIDVERLINTVKLNFDASITTKQLILAGTIQFATAIRAAKSELENA 647
            D T IPCLYVFV+IKIDVE LI+T+KLN       K +ILAGTIQFA+AIR AK ELE  
Sbjct: 131  DATKIPCLYVFVEIKIDVECLISTIKLNLK---DYKSVILAGTIQFASAIREAKPELERL 187

Query: 648  GFRVMIPQSKPLSAGEVLGCTAPTIPMAQF--NESKVVLVFVADGRFHLEAFMIANPRIK 821
            G   +IPQSKPLSAGEVLGCTAP I         S+ V+VFVADGRFHLEAFMIANP I 
Sbjct: 188  GLYALIPQSKPLSAGEVLGCTAPKISSNSMVGGGSETVVVFVADGRFHLEAFMIANPGIS 247

Query: 822  AFRYDPYIGSLFLEEYDHKGMKECRKNAILKSRDAKNWGIVLGTLGRQGNPRILDRLEKK 1001
             FRYDPY+G LFLEEYDH+GMKE RK AI  +++A+NWGIVLGTLGRQGNPRILDRLEKK
Sbjct: 248  TFRYDPYMGKLFLEEYDHRGMKEVRKGAIQMAKEARNWGIVLGTLGRQGNPRILDRLEKK 307

Query: 1002 MGEKGLSWTIVLMSELSPVRIALFGDSIDAWIQIACPRLSIDWGDAFVKPLLTPFEAEIA 1181
            M EKG S+T+VLMSE+SPVRIALF D IDAWIQIACPRLSIDWG+AF KPLLTPFEAEIA
Sbjct: 308  MREKGFSYTVVLMSEISPVRIALFEDCIDAWIQIACPRLSIDWGEAFKKPLLTPFEAEIA 367

Query: 1182 LGDI 1193
            LGD+
Sbjct: 368  LGDL 371