BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00000032
         (1100 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAA72009.1| invertase [Cichorium intybus]                         620   e-175
emb|CAD49079.1| fructan 1-exohydrolase [Campanula rapunculoides]      524   e-146
gb|AFH77950.1| cell wall invertase [Manihot esculenta]                494   e-137
pdb|1ST8|A Chain A, Crystal Structure Of Fructan 1-Exohydrolase ...   488   e-135
emb|CAC37922.1| fructan 1-exohydrolase IIa [Cichorium intybus] g...   488   e-135

>emb|CAA72009.1| invertase [Cichorium intybus]
          Length = 550

 Score =  620 bits (1600), Expect = e-175
 Identities = 290/368 (78%), Positives = 329/368 (89%), Gaps = 2/368 (0%)
 Frame = +3

Query: 3    PFDIKSCWSGSATILPGNKPVMLYTGIDSQDRQVQNLAVPKNLSDPYLREWVKYTGNPVI 182
            PFDI  C SGSAT+LPGNKP+MLYTGID+++RQVQNLAVPK+LSDPYLREWVK+TGNP+I
Sbjct: 88   PFDINGCLSGSATVLPGNKPIMLYTGIDTENRQVQNLAVPKDLSDPYLREWVKHTGNPII 147

Query: 183  DIPEGIQHDDFRDPTTAWLADDGKWRIIVGSQKDKTGIAFLYQSEDFINWTRFDSPFHMV 362
             +PE IQ DDFRDPTT WL +DG WR++VGSQKDKTGIAFLY S DF+NWT+ DSP H V
Sbjct: 148  SLPEEIQPDDFRDPTTTWLEEDGTWRLLVGSQKDKTGIAFLYHSGDFVNWTKSDSPLHKV 207

Query: 363  AGTGIWECPDFFPVWVDSKNGVDTSVINP--RVKHVLKVGLFDYSKDFYMIGNYSPEKEN 536
            +GTG+WEC DFFPVWVDS NGVDTS+INP  RVKHVLK+G+ D+ KD Y+IG YS +KEN
Sbjct: 208  SGTGMWECVDFFPVWVDSTNGVDTSIINPSNRVKHVLKLGIQDHGKDCYLIGKYSADKEN 267

Query: 537  YIPQNELTLTTSRYDYGKYYASKSFFDPVKNRRIVMAWVNESDSVADDIAKGWSGLHSFP 716
            Y+P++ELTL+T R DYG YYASKSFFDPVKNRRI+ AWVNESDS AD IA+GWSG+ SFP
Sbjct: 268  YVPEDELTLSTLRLDYGMYYASKSFFDPVKNRRIMTAWVNESDSEADVIARGWSGVQSFP 327

Query: 717  RSLWLDQNQKQLVQWPIEEIEMLHENEVSFSNKKLEGGSLHEVVGVTASQADVKISFKLS 896
            RSLWLD+NQKQL+QWPIEEIEMLH+NEVSF NKKL+GGS  EV+G+TASQADVKISFKL+
Sbjct: 328  RSLWLDKNQKQLLQWPIEEIEMLHQNEVSFHNKKLDGGSSLEVLGITASQADVKISFKLA 387

Query: 897  NLEEAEELDRSWIDPQLICSEKDASNKGKFGPFGLLALASDDLTEQTAVFFRVFRNNGRY 1076
            NLEEAEELD SW+DPQLICSE DAS KGKFGPFGLLALAS DL EQTA+FFRVFR NGRY
Sbjct: 388  NLEEAEELDPSWVDPQLICSENDASKKGKFGPFGLLALASSDLREQTAIFFRVFRKNGRY 447

Query: 1077 IVLMCSDQ 1100
            +VLMCSDQ
Sbjct: 448  VVLMCSDQ 455


>emb|CAD49079.1| fructan 1-exohydrolase [Campanula rapunculoides]
          Length = 578

 Score =  524 bits (1349), Expect = e-146
 Identities = 250/369 (67%), Positives = 302/369 (81%), Gaps = 3/369 (0%)
 Frame = +3

Query: 3    PFDIKSCWSGSATILPGNKPVMLYTGIDSQDRQVQNLAVPKNLSDPYLREWVKYTGNPVI 182
            P DI SC+SGSAT LPG KPVMLYTG+D++ RQVQNLAVPKNLSDP+LREWVK+  NP++
Sbjct: 113  PADINSCFSGSATFLPGYKPVMLYTGLDTEKRQVQNLAVPKNLSDPFLREWVKHKANPIM 172

Query: 183  DIPEGIQHDDFRDPTTAWLADDGKWRIIVGSQKDKTGIAFLYQSEDFINWTRFDSPFHMV 362
              PEG++ DDFRDP+TAWL  DGKWR++VGS+K+  G+A+LYQS+DF+ W RFD P   +
Sbjct: 173  TTPEGVKADDFRDPSTAWLGYDGKWRVLVGSKKNDLGVAYLYQSKDFVKWERFDYPLMSM 232

Query: 363  AGTGIWECPDFFPVWVDSKNGVDTS-VINPRVKHVLKVGLFDYSKDFYMIGNYSPEKENY 539
              T  WECPDFFPV V S NG+DTS VINP VKHV+KVG      D+Y IG  S E++NY
Sbjct: 233  METSTWECPDFFPVSVSSTNGLDTSGVINPGVKHVVKVGFNGI--DWYTIGTLS-ERDNY 289

Query: 540  IPQNELTLTT--SRYDYGKYYASKSFFDPVKNRRIVMAWVNESDSVADDIAKGWSGLHSF 713
            +P+N L   +   RYDYGK+YASKSF+D  K RR++  W++E+D+  DD+A+GWSGL + 
Sbjct: 290  VPENGLKGNSLDMRYDYGKFYASKSFYDNAKQRRVLWGWISEADAQEDDVARGWSGLQAV 349

Query: 714  PRSLWLDQNQKQLVQWPIEEIEMLHENEVSFSNKKLEGGSLHEVVGVTASQADVKISFKL 893
            PRS+WLD+N KQLVQWP+EEIE L ENEV FSNK+LEGGSL EV G+TASQADVKISFKL
Sbjct: 350  PRSVWLDRNGKQLVQWPVEEIEKLRENEVKFSNKELEGGSLFEVEGITASQADVKISFKL 409

Query: 894  SNLEEAEELDRSWIDPQLICSEKDASNKGKFGPFGLLALASDDLTEQTAVFFRVFRNNGR 1073
            SNLEEAEELD SW DPQL+CSE   S+KGK+GPFGLLALASDDLTEQTA+FFRVF ++G+
Sbjct: 410  SNLEEAEELDPSWTDPQLLCSEMGVSSKGKYGPFGLLALASDDLTEQTAIFFRVFSSHGK 469

Query: 1074 YIVLMCSDQ 1100
            Y+VLMCSDQ
Sbjct: 470  YVVLMCSDQ 478


>gb|AFH77950.1| cell wall invertase [Manihot esculenta]
          Length = 592

 Score =  494 bits (1271), Expect = e-137
 Identities = 229/368 (62%), Positives = 286/368 (77%), Gaps = 2/368 (0%)
 Frame = +3

Query: 3    PFDIKSCWSGSATILPGNKPVMLYTGIDSQDRQVQNLAVPKNLSDPYLREWVKYTGNPVI 182
            PFDIKSCWSGS TILPGNKP +LYTGI++ D QVQNLA+PKNLSDP L+EW+K++ NPVI
Sbjct: 111  PFDIKSCWSGSVTILPGNKPAILYTGINANDTQVQNLAMPKNLSDPLLKEWIKFSQNPVI 170

Query: 183  DIPEGIQHDDFRDPTTAWLADDGKWRIIVGSQKDKTGIAFLYQSEDFINWTRFDSPFHMV 362
              P G+  DDFRDPTTAW+  +GKW +IVGS  +  G+A LYQSEDF+NW  +  P H  
Sbjct: 171  TPPTGVAKDDFRDPTTAWVDAEGKWNVIVGSLINDRGMAILYQSEDFVNWRMYKDPLHSK 230

Query: 363  AGTGIWECPDFFPVWVDSKNGVDTSVINPRVKHVLKVGLFDYSKDFYMIGNYSPEKENYI 542
              TG+WECPDFFPV ++S NGVDTSV+NP VKHV+K      S D+Y+IG Y P  + YI
Sbjct: 231  EKTGMWECPDFFPVSINSTNGVDTSVMNPSVKHVMKASF--NSHDYYIIGTYVPGVQKYI 288

Query: 543  PQNELTLTTS--RYDYGKYYASKSFFDPVKNRRIVMAWVNESDSVADDIAKGWSGLHSFP 716
            P  + T T +  RYDYGK+YASK+FFD +KNRRI+  WVNESDS  DD+ KGWSGL SFP
Sbjct: 289  PDTDFTSTNTDLRYDYGKFYASKTFFDSIKNRRILWGWVNESDSSEDDVRKGWSGLQSFP 348

Query: 717  RSLWLDQNQKQLVQWPIEEIEMLHENEVSFSNKKLEGGSLHEVVGVTASQADVKISFKLS 896
            R +WL+++  QLVQWP+EEI  LH+ +VS  NKKL+GGS+ E+ G+TASQAD +I F+L 
Sbjct: 349  RQIWLNRSGSQLVQWPVEEINKLHDKQVSIQNKKLDGGSVLEIPGITASQADAEIVFELP 408

Query: 897  NLEEAEELDRSWIDPQLICSEKDASNKGKFGPFGLLALASDDLTEQTAVFFRVFRNNGRY 1076
             LEEAE L  + +DPQ +C++ +AS +G+ GPFGLLALA++DLTEQTA+FFR+FR+   Y
Sbjct: 409  ELEEAELLKSTPVDPQQMCTDANASVRGRLGPFGLLALATEDLTEQTAIFFRIFRHENTY 468

Query: 1077 IVLMCSDQ 1100
            IVLMCSDQ
Sbjct: 469  IVLMCSDQ 476


>pdb|1ST8|A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From
            Cichorium Intybus gi|114793383|pdb|2ADD|A Chain A,
            Crystal Structure Of Fructan 1-Exohydrolase Iia From
            Cichorium Intybus In Complex With Sucrose
            gi|114793384|pdb|2ADE|A Chain A, Crystal Structure Of
            Fructan 1-Exohydrolase Iia From Cichorium Intybus In
            Complex With Fructose gi|114793389|pdb|2AEY|A Chain A,
            Crystal Structure Of Fructan 1-Exohydrolase Iia From
            Cichorium Intybus In Complex With 2,5
            Dideoxy-2,5-Immino-D-Mannitol
          Length = 543

 Score =  488 bits (1255), Expect = e-135
 Identities = 231/368 (62%), Positives = 287/368 (77%), Gaps = 5/368 (1%)
 Frame = +3

Query: 9    DIKSCWSGSATILPGNKPVMLYTGIDSQDRQVQNLAVPKNLSDPYLREWVKYTGNPVIDI 188
            D KSCWSGSATILPGN P MLYTG DS+ RQVQ+LA PKNLSDP+LREWVK+  NP+I  
Sbjct: 77   DSKSCWSGSATILPGNIPAMLYTGSDSKSRQVQDLAWPKNLSDPFLREWVKHPKNPLITP 136

Query: 189  PEGIQHDDFRDPTTAWLADDGKWRIIVGSQKDKTGIAFLYQSEDFINWTRFDSPFHMVAG 368
            PEG++ D FRDP+TAWL  DG WRI+VG  +D  G+AFLYQS DF+NW R+D P      
Sbjct: 137  PEGVKDDCFRDPSTAWLGPDGVWRIVVGGDRDNNGMAFLYQSTDFVNWKRYDQPLSSADA 196

Query: 369  TGIWECPDFFPVWVDSKNGVDTSVINPRVKHVLKVGLFDYSKDFYMIGNYSPEKENYIPQ 548
            TG WECPDF+PV ++S NG+DTSV    V+HV+K G   +  D+Y IG YSP++EN++PQ
Sbjct: 197  TGTWECPDFYPVPLNSTNGLDTSVYGGSVRHVMKAGFEGH--DWYTIGTYSPDRENFLPQ 254

Query: 549  NELTLTTS----RYDYGKYYASKSFFDPVKNRRIVMAWVNESDSVADDIAKGWSGLHSFP 716
            N L+LT S    RYDYG++YASKSFFD  KNRR++ AWV E+DS ADDI KGW+GL SFP
Sbjct: 255  NGLSLTGSTLDLRYDYGQFYASKSFFDDAKNRRVLWAWVPETDSQADDIEKGWAGLQSFP 314

Query: 717  RSLWLDQNQKQLVQWPIEEIEMLHENEVSFSNKKLEGGSLHEVVGVTASQADVKISFKLS 896
            R+LW+D+N KQL+QWP+EEIE L +N+V+  NK L+ GS+ E+ G+ ASQADV ISFKL 
Sbjct: 315  RALWIDRNGKQLIQWPVEEIEELRQNQVNLQNKNLKPGSVLEIHGIAASQADVTISFKLE 374

Query: 897  NLEEAEELDRSWIDPQLICSEKDASNKGKFGPFGLLALASDDLTEQTAVFFRVFRNN-GR 1073
             L+EAE LD + +DPQ +C+E+ AS++G  GPFGLLA+AS DL EQ+A+FFRVF+N  GR
Sbjct: 375  GLKEAEVLDTTLVDPQALCNERGASSRGALGPFGLLAMASKDLKEQSAIFFRVFQNQLGR 434

Query: 1074 YIVLMCSD 1097
            Y VLMCSD
Sbjct: 435  YSVLMCSD 442


>emb|CAC37922.1| fructan 1-exohydrolase IIa [Cichorium intybus]
            gi|32492892|gb|AAP85536.1| fructan 1-exohydrolase IIa
            [Cichorium intybus]
          Length = 581

 Score =  488 bits (1255), Expect = e-135
 Identities = 231/368 (62%), Positives = 287/368 (77%), Gaps = 5/368 (1%)
 Frame = +3

Query: 9    DIKSCWSGSATILPGNKPVMLYTGIDSQDRQVQNLAVPKNLSDPYLREWVKYTGNPVIDI 188
            D KSCWSGSATILPGN P MLYTG DS+ RQVQ+LA PKNLSDP+LREWVK+  NP+I  
Sbjct: 115  DSKSCWSGSATILPGNIPAMLYTGSDSKSRQVQDLAWPKNLSDPFLREWVKHPKNPLITP 174

Query: 189  PEGIQHDDFRDPTTAWLADDGKWRIIVGSQKDKTGIAFLYQSEDFINWTRFDSPFHMVAG 368
            PEG++ D FRDP+TAWL  DG WRI+VG  +D  G+AFLYQS DF+NW R+D P      
Sbjct: 175  PEGVKDDCFRDPSTAWLGPDGVWRIVVGGDRDNNGMAFLYQSTDFVNWKRYDQPLSSADA 234

Query: 369  TGIWECPDFFPVWVDSKNGVDTSVINPRVKHVLKVGLFDYSKDFYMIGNYSPEKENYIPQ 548
            TG WECPDF+PV ++S NG+DTSV    V+HV+K G   +  D+Y IG YSP++EN++PQ
Sbjct: 235  TGTWECPDFYPVPLNSTNGLDTSVYGGSVRHVMKAGFEGH--DWYTIGTYSPDRENFLPQ 292

Query: 549  NELTLTTS----RYDYGKYYASKSFFDPVKNRRIVMAWVNESDSVADDIAKGWSGLHSFP 716
            N L+LT S    RYDYG++YASKSFFD  KNRR++ AWV E+DS ADDI KGW+GL SFP
Sbjct: 293  NGLSLTGSTLDLRYDYGQFYASKSFFDDAKNRRVLWAWVPETDSQADDIEKGWAGLQSFP 352

Query: 717  RSLWLDQNQKQLVQWPIEEIEMLHENEVSFSNKKLEGGSLHEVVGVTASQADVKISFKLS 896
            R+LW+D+N KQL+QWP+EEIE L +N+V+  NK L+ GS+ E+ G+ ASQADV ISFKL 
Sbjct: 353  RALWIDRNGKQLIQWPVEEIEELRQNQVNLQNKNLKPGSVLEIHGIAASQADVTISFKLE 412

Query: 897  NLEEAEELDRSWIDPQLICSEKDASNKGKFGPFGLLALASDDLTEQTAVFFRVFRNN-GR 1073
             L+EAE LD + +DPQ +C+E+ AS++G  GPFGLLA+AS DL EQ+A+FFRVF+N  GR
Sbjct: 413  GLKEAEVLDTTLVDPQALCNERGASSRGALGPFGLLAMASKDLKEQSAIFFRVFQNQLGR 472

Query: 1074 YIVLMCSD 1097
            Y VLMCSD
Sbjct: 473  YSVLMCSD 480