BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00043194
         (1674 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAN05009.1| ribosome-inactivating protein [Binary vector pGV4...   424   e-116
gb|AET98835.1| type 2 ribosome-inactivating protein [Cinnamomum ...   398   e-108
gb|AAF37219.1|AF213984_1 ribosome inactivating protein RIPt [Pol...   383   e-104
gb|AAK82460.1| type 2 ribosome-inactivating protein cinnamomin I...   381   e-103
pdb|2VLC|A Chain A, Crystal Structure Of Natural Cinnamomin (Iso...   377   e-102

>gb|AAN05009.1| ribosome-inactivating protein [Binary vector pGV4945]
          Length = 589

 Score =  424 bits (1091), Expect = e-116
 Identities = 239/519 (46%), Positives = 321/519 (61%), Gaps = 22/519 (4%)
 Frame = +3

Query: 27   HGIAVTRPPTN-IPLQERFVQVQLENGASRGAVITVIIDTVNVYVVGYLVGSTLRPTLRY 203
            +GI V   P N +P++ERFV V L    ++  ++   +D  N+YV  +   +       +
Sbjct: 76   NGIPVLLNPANPVPVRERFVMVHLTGRNNKTVILA--LDVTNLYVAAFSANNVAYFFSDF 133

Query: 204  LDLDQPDDREALFQAFPSTQYTHNRLGFAGNYDSLP-------GRDSHELGHAALNNAIS 362
              L+    RE LF    S      RL F   Y SL        GR++  LG   L+ A +
Sbjct: 134  TALE----RENLFSGMLSI-----RLSFTSKYVSLEHKAGVGGGRENISLGPTPLDEATN 184

Query: 363  NLYYRRSERSA-----MLVIIQMVAEAVRIRYIEHLIRRSMLARSMTGENFIPDPRAISM 527
            +L+  RS   A     +LV+IQMV+EA R R+IE  +RRS  A       F PD   +SM
Sbjct: 185  SLWSGRSVTEASIAEALLVVIQMVSEAARFRHIEERVRRSFTAADHQ-LTFRPDGLMLSM 243

Query: 528  ENRWSDLSQQIQWSGESGVFLRTIQVRSVLDEDVPIRSVVGVMRQAALALMLYQCNRK-- 701
            EN WS +S ++Q   E G+F+  +Q+     + + + +   + R   +AL+L++C     
Sbjct: 244  ENEWSSMSLEVQRPIEGGIFIGVVQLEDESFKPLRVDNFNTLSRYTMVALLLFRCGHPPA 303

Query: 702  -AIIMPVPVAVGA------DEQCPYGEPTTNIIGRDGQCVDVRDNQYNNGNSIILWACGN 860
             A I+ +PV +        DE C  G PT  I GRDG CVDVRD + N+GN I LW CG 
Sbjct: 304  AAQIIRMPVVLAGREYDDDDETCTVGAPTRRISGRDGLCVDVRDGRDNDGNPIQLWPCG- 362

Query: 861  AQRNQLWTFKSDGTIRSNGKCLTTYGYTSGNYIMIFDCDTAVREATQWILYNAGTIMNPR 1040
            AQRNQ WTF +DGTIRS GKC+TTYGY+ GNY+MIF+C TAV +AT+W++   G+I NPR
Sbjct: 363  AQRNQQWTFHTDGTIRSMGKCMTTYGYSPGNYVMIFNCRTAVPDATKWVVSIDGSITNPR 422

Query: 1041 SGLVIAAETSTQGTVLTVAEDNNSSRQAWSAGNYTQPTINYISGFREMCLQANGANARVW 1220
            SGLV+ A  +  GT LTV  + +S+RQ WS G+  QP + YI GF++MCLQ N  + RVW
Sbjct: 423  SGLVLTALQAAHGTTLTVNTNTHSARQGWSVGDDVQPIVTYIVGFKDMCLQRN--STRVW 480

Query: 1221 LANCVIGTEPRQQWALYGDSTIRLYSDRTLCVTSDGHESLDRIILLKCQGWGNQRWTFMA 1400
            L +C +  + +Q+WALY D TIR+ SDR+LCVTS+GH S D II+L C G GNQRW F  
Sbjct: 481  LEDCAVDRQ-QQRWALYSDGTIRVDSDRSLCVTSNGHSSRDVIIILTCDGRGNQRWVFNT 539

Query: 1401 DGTILNPNARLVMDVRGSDVSLQEIILYRPTGNPNQNWL 1517
            DGTILNPNA+LVMDVRGSDV+L++IILY+PTGNPNQ W+
Sbjct: 540  DGTILNPNAQLVMDVRGSDVALRQIILYQPTGNPNQQWM 578


>gb|AET98835.1| type 2 ribosome-inactivating protein [Cinnamomum camphora]
          Length = 585

 Score =  398 bits (1022), Expect = e-108
 Identities = 237/536 (44%), Positives = 317/536 (59%), Gaps = 31/536 (5%)
 Frame = +3

Query: 3    RLASGTDAHGIAVTRPPTNIPLQERFVQVQLENGASRGAVITVIIDTVNVYVVGYLVGST 182
            +LASG + HGI VTR  + +P  +RFV V+L N A+   V T+ ++  + YVV Y  GS 
Sbjct: 64   QLASGEEPHGIPVTRERSTVPDSQRFVLVELSNWAADSPV-TLALNVTDAYVVAYRTGSR 122

Query: 183  LRPTLRYLDLDQPDDREALFQAFPSTQYTHNRLGFAGNYDSLPG-----RDSHELGHAAL 347
                  +L  D PD   A+   FP TQ     L F GNY+ L       RD+ ELG   L
Sbjct: 123  SF----FLREDNPDP--AIDNLFPDTQ--RQTLRFGGNYNDLEDAAGQRRDTIELGMEPL 174

Query: 348  NNAISNLYYRRSERS-----AMLVIIQMVAEAVRIRYIEHLIRRSMLARSMTGENFIPDP 512
             NAIS L    ++       +++V+IQMVAEAVR RYIE  + +S+     T + F+PDP
Sbjct: 175  ENAISTLRVSNTDPDGPIARSLIVVIQMVAEAVRFRYIEGRVSQSI----GTAQVFVPDP 230

Query: 513  RAISMENRWSDLSQQIQWSGESGVFLRTIQVRSVLDEDVPIRSVVGVMRQAALALMLYQC 692
                +EN+W  LS+ +Q S + GVF R +++RS  +  V + SV   +  + LA+ML+ C
Sbjct: 231  AMRRLENKWKKLSKAVQESNQGGVFSRPVELRSRSNNPVNVDSVSHPVI-SGLAIMLFSC 289

Query: 693  NR----------------KAIIMPVPVAVGAD--EQCPYGEPTTNIIGRDGQCVDVRDNQ 818
                              + I++ V VA G D  + C   EPT  I GR+G CVDVRD Q
Sbjct: 290  RSSDRASSDQFIDHLLIIRPIVVDVDVATGVDNDDTCDDPEPTVRISGRNGLCVDVRDGQ 349

Query: 819  YNNGNSIILWACG-NAQRNQLWTFKSDGTIRSNGKCLTTYGYTSGNYIMIFDCDTAVREA 995
            YN+GN I LW C  N+  NQLWT + DGTIRSNGKCLTTYG + GNY+MIFDC TAV  A
Sbjct: 350  YNDGNPIQLWPCKQNSDVNQLWTLRKDGTIRSNGKCLTTYGSSPGNYVMIFDCTTAVTVA 409

Query: 996  TQWILYNAGTIMNPRSGLVIAAETSTQGTVLTVAEDNNSSRQAWSAGNYTQPTINYISGF 1175
            T W ++  GTI NP+S LV++AE+   GT LTV  +  +SRQ W AGN T+P +  I+GF
Sbjct: 410  TIWHVWANGTIFNPQSALVLSAESGNSGTTLTVQNNIYASRQGWLAGNNTEPFVTSIAGF 469

Query: 1176 REMCLQANGANARVWLANCVIGTEPRQQWALYGDSTIRLYSDRTLCVTSDGHESLDRIIL 1355
             ++C+QANG    +W+  C   ++  Q+WALY D +IR   +R  C+TS  + S   II+
Sbjct: 470  NDLCMQANG--DAMWVVECE-SSKAEQKWALYPDGSIRPQQNRDRCLTSSDNHSKGSIII 526

Query: 1356 LK--CQGWGNQRWTFMADGTILNPNARLVMDVRGSDVSLQEIILYRPTGNPNQNWL 1517
            +     G   QRW FM DGTILN    +VMDV+GSD SL +II++  TGNPNQ WL
Sbjct: 527  ISGCSSGSEGQRWVFMNDGTILNLKNGMVMDVKGSDPSLHQIIIWEFTGNPNQKWL 582


>gb|AAF37219.1|AF213984_1 ribosome inactivating protein RIPt [Polygonatum multiflorum]
          Length = 603

 Score =  383 bits (984), Expect = e-104
 Identities = 222/522 (42%), Positives = 304/522 (58%), Gaps = 32/522 (6%)
 Frame = +3

Query: 48   PPTNIPLQERFVQVQLENGASRGAVITVIIDTVNVYVVGYLVGSTLRPTLRYLDLDQPDD 227
            P   +P++ERFV+V L      G  + + +D  N+YV  +   +       +  L+    
Sbjct: 83   PAHPVPVRERFVKVHLTG--RNGKTVILALDVTNLYVAAFSANNVAYFFRDFSALE---- 136

Query: 228  REALFQAFPSTQYTHNRLGFAGNYDSLP-----GRDSHELGHAALNNAISNLYY------ 374
            RE LF    +      RL F  NY SL      GR++  LG   L+ A +   +      
Sbjct: 137  RENLFSGMLTI-----RLSFTSNYVSLEHKAGVGRENISLGPTPLDEACTKSLWSGTTVT 191

Query: 375  RRSERSAMLVIIQMVAEAVRIRYIEHLIRRSMLARSMTGENFIPDPRAISMENRWSDLSQ 554
              S   A+LV+IQMV+EA R R+IE  +RRS  A       F PD   +SMEN W  +S 
Sbjct: 192  EASIAKALLVVIQMVSEAARFRHIEERVRRSFTAADHDQLTFRPDGLMLSMENEWPSMSL 251

Query: 555  QIQWSGESGVFLRTIQVRSVLDEDVPIRSVVGVMRQAALALMLYQCNR------------ 698
            ++Q S E G+F+  +Q++    + + + +   + R   +AL+L++C              
Sbjct: 252  EVQRSIEGGIFIGVVQLQDESFQPLRVDNFNTLSRYTMVALLLFRCGHPRATAGTSSTTP 311

Query: 699  ---KAIIMPVPVAVGAD----EQCPYGEPTTNIIGRDGQCVDVRDNQYNNGNSIILWACG 857
               + I MPV V  G +    E C  GEPT  I G DG C+DVR+   N+G  I LW CG
Sbjct: 312  AAAQIIRMPVDVLAGEEYYDEETCTVGEPTRRISGLDGLCMDVRNESNNDGIPIQLWPCG 371

Query: 858  NAQRNQLWTFKSDGTIRSNGKCLTTYGYTSGNYIMIFDCDTA-VREATQWILYNAGTIMN 1034
             AQRNQ WTF +DGTI+S GKC+T+ GY  G+Y+MIF+C TA V +AT+W++   G+I N
Sbjct: 372  -AQRNQQWTFHTDGTIQSMGKCMTSNGYHPGDYVMIFNCSTAPVPDATKWVVSIDGSITN 430

Query: 1035 PRSGLVIAAETSTQGTVLTVAEDNNSSRQAWSAGNYTQPTINYISGFREMCLQANGAN-A 1211
            P SGLV+ A  + Q T+L V  + +S++Q  S G+  +P + YI GF+ MCLQ N  N  
Sbjct: 431  PHSGLVLTAPQAAQTTILLVVRNTHSAKQGRSVGDDVEPIVTYIVGFKYMCLQGNNENNT 490

Query: 1212 RVWLANCVIGTEPRQQWALYGDSTIRLYSDRTLCVTSDGHESLDRIILLKCQGWGNQRWT 1391
            RVWL +C +   P+Q WALY D TIR+ SDR+LCVTSDGH S D II+L C G  NQR  
Sbjct: 491  RVWLEDCAVD-RPQQWWALYSDGTIRVDSDRSLCVTSDGHSSRDAIIILTCDGGINQRLV 549

Query: 1392 FMADGTILNPNARLVMDVRGSDVSLQEIILYRPTGNPNQNWL 1517
            F  DGTILNPNA+LVMDVR S+V+L++IILY+PTGNPNQ W+
Sbjct: 550  FNTDGTILNPNAQLVMDVRQSNVALRQIILYQPTGNPNQQWM 591


>gb|AAK82460.1| type 2 ribosome-inactivating protein cinnamomin III precursor
            [Cinnamomum camphora]
          Length = 580

 Score =  381 bits (978), Expect = e-103
 Identities = 235/538 (43%), Positives = 316/538 (58%), Gaps = 33/538 (6%)
 Frame = +3

Query: 3    RLASGTDAHGIAVTRPPTNIPLQERFVQVQLENGASRGAVITVIIDTVNVYVVGYLVGST 182
            +LASG + HGI V R  + +P  +RF+ V+L N A+   V T+ +D  N YVV Y  GS 
Sbjct: 58   QLASGEEPHGIPVMRERSTVPDSKRFILVELSNWAADSPV-TLAVDVTNAYVVAYRTGSQ 116

Query: 183  LRPTLRYLDLDQPDDREALFQAFPSTQ-YTHNRLGFAGNYDSLPG-----RDSHELGHAA 344
                  +L  D PD   A+    P T+ YT     F+G+Y  L       R+   LG   
Sbjct: 117  SF----FLREDNPDP--AIENLLPDTKRYT---FPFSGSYTDLERVAGERREEILLGMDP 167

Query: 345  LNNAIS-----NLYYRRSERSAMLVIIQMVAEAVRIRYIEHLIRRSMLARSMTGENFIPD 509
            L NAIS     NL  +R+   +++V+IQMVAEAVR R+IE+ +R S+    M    F PD
Sbjct: 168  LENAISALWISNLNQQRALARSLIVVIQMVAEAVRFRFIEYRVRESITRAEM----FRPD 223

Query: 510  PRAISMENRWSDLSQQIQWSGESGVFLRTIQVRSVLDEDVPIRSVVGVMRQAALALMLYQ 689
            P  +S+EN+WS LS  +Q S + GVF   +++RS+ ++ V + SV   +  + LA+ML+ 
Sbjct: 224  PAMLSLENKWSALSNAVQQSNQGGVFSSPVELRSISNKPVYVGSVSDRVI-SGLAIMLFI 282

Query: 690  C------------NRKAIIMPV-----PVAVGAD--EQCPYGEPTTNIIGRDGQCVDVRD 812
            C            +   +I P+      VA  AD  + C   EPT  I GR+G CVDVRD
Sbjct: 283  CRSTDRASSDQFIDHLLMIRPILADVADVATDADNDDTCADPEPTVRISGRNGLCVDVRD 342

Query: 813  NQYNNGNSIILWACG-NAQRNQLWTFKSDGTIRSNGKCLTTYGYTSGNYIMIFDCDTAVR 989
             +YNNGN I LW C  N+  NQLWT + DGTIRSNGKCLTT GY++G+Y+MI+DC T V 
Sbjct: 343  GKYNNGNPIQLWPCKQNSDVNQLWTLRRDGTIRSNGKCLTTNGYSAGDYVMIYDCRTPVT 402

Query: 990  EATQWILYNAGTIMNPRSGLVIAAETSTQGTVLTVAEDNNSSRQAWSAGNYTQPTINYIS 1169
             A+ W  +  GTI+NP+S LV++AE+    T LTV  D  +SRQ W AGN T+P +  I 
Sbjct: 403  AASIWQFWANGTIINPQSALVLSAESGNPRTTLTVQADIYASRQGWLAGNNTEPFVTSIV 462

Query: 1170 GFREMCLQANGANARVWLANCVIGTEPRQQWALYGDSTIRLYSDRTLCVTS-DGHESLDR 1346
            GF ++C+QANG    +W+  C   ++  Q+WALY D +IR + DR  C+TS D H     
Sbjct: 463  GFNDLCMQANG--DAMWVVECE-SSKAEQKWALYPDGSIRPHQDRDRCLTSTDNHSQGSI 519

Query: 1347 IILLKCQ-GWGNQRWTFMADGTILNPNARLVMDVRGSDVSLQEIILYRPTGNPNQNWL 1517
            II+  C  G   QRW FM DGTILN    LVMDV+GSD SL +II++  TG PNQ WL
Sbjct: 520  IIISSCSPGSEGQRWVFMNDGTILNLKNGLVMDVKGSDPSLHQIIIWPATGKPNQKWL 577


>pdb|2VLC|A Chain A, Crystal Structure Of Natural Cinnamomin (Isoform Iii)
            gi|223365706|pdb|2VLC|B Chain B, Crystal Structure Of
            Natural Cinnamomin (Isoform Iii)
          Length = 570

 Score =  377 bits (969), Expect = e-102
 Identities = 234/538 (43%), Positives = 315/538 (58%), Gaps = 33/538 (6%)
 Frame = +3

Query: 3    RLASGTDAHGIAVTRPPTNIPLQERFVQVQLENGASRGAVITVIIDTVNVYVVGYLVGST 182
            +LASG + HGI V R  + +P  +RF+ V+L N A+   V T+ +D  N YVV Y  GS 
Sbjct: 48   QLASGEEPHGIPVMRERSTVPDSKRFILVELSNWAADSPV-TLAVDVTNAYVVAYRTGSQ 106

Query: 183  LRPTLRYLDLDQPDDREALFQAFPSTQ-YTHNRLGFAGNYDSLPG-----RDSHELGHAA 344
                  +L  D PD   A+    P T+ YT     F+G+Y  L       R+   LG   
Sbjct: 107  SF----FLREDNPDP--AIENLLPDTKRYT---FPFSGSYTDLERVAGERREEILLGMDP 157

Query: 345  LNNAIS-----NLYYRRSERSAMLVIIQMVAEAVRIRYIEHLIRRSMLARSMTGENFIPD 509
            L NAIS     NL  +R+   +++V+IQMVAEAVR R+IE+ +R S+    M    F PD
Sbjct: 158  LENAISALWISNLNQQRALARSLIVVIQMVAEAVRFRFIEYRVRESISRAEM----FRPD 213

Query: 510  PRAISMENRWSDLSQQIQWSGESGVFLRTIQVRSVLDEDVPIRSVVGVMRQAALALMLYQ 689
            P  +S+EN+WS LS  +Q S + GVF   +++RS+ ++ V + SV   +  + LA+ML+ 
Sbjct: 214  PAMLSLENKWSALSNAVQQSNQGGVFSSPVELRSISNKPVYVGSVSDRVI-SGLAIMLFI 272

Query: 690  C------------NRKAIIMPV-----PVAVGAD--EQCPYGEPTTNIIGRDGQCVDVRD 812
            C            +   +I P+      VA  AD  + C   EPT  I GR+G CV VRD
Sbjct: 273  CRSTDRASSDQFIDHLLMIRPILADVADVATDADNDDTCADPEPTVRISGRNGLCVRVRD 332

Query: 813  NQYNNGNSIILWACG-NAQRNQLWTFKSDGTIRSNGKCLTTYGYTSGNYIMIFDCDTAVR 989
             +YNNGN I LW C  N+  NQLWT + DGTIRSNGKCLTT GY++G+Y+MI+DC T V 
Sbjct: 333  GKYNNGNPIQLWPCKQNSDVNQLWTLRRDGTIRSNGKCLTTNGYSAGDYVMIYDCRTPVT 392

Query: 990  EATQWILYNAGTIMNPRSGLVIAAETSTQGTVLTVAEDNNSSRQAWSAGNYTQPTINYIS 1169
             A+ W  +  GTI+NP+S LV++AE+    T LTV  D  +SRQ W AGN T+P +  I 
Sbjct: 393  AASIWQFWANGTIINPQSALVLSAESGNPRTTLTVQADIYASRQGWLAGNNTEPFVTSIV 452

Query: 1170 GFREMCLQANGANARVWLANCVIGTEPRQQWALYGDSTIRLYSDRTLCVTS-DGHESLDR 1346
            GF ++C+QANG    +W+  C   ++  Q+WALY D +IR + DR  C+TS D H     
Sbjct: 453  GFNDLCMQANG--DAMWVVECE-SSKAEQKWALYPDGSIRPHQDRDRCLTSTDNHSQGSI 509

Query: 1347 IILLKCQ-GWGNQRWTFMADGTILNPNARLVMDVRGSDVSLQEIILYRPTGNPNQNWL 1517
            II+  C  G   QRW FM DGTILN    LVMDV+GSD SL +II++  TG PNQ WL
Sbjct: 510  IIISSCSPGSEGQRWVFMNDGTILNLKNGLVMDVKGSDPSLHQIIIWPATGKPNQKWL 567