BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00032849
         (1457 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]    813   0.0  
sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desatur...   811   0.0  
gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]        794   0.0  
ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vi...   677   0.0  
ref|XP_002302613.1| predicted protein [Populus trichocarpa] gi|2...   663   0.0  

>gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]
          Length = 466

 Score =  813 bits (2099), Expect = 0.0
 Identities = 381/419 (90%), Positives = 402/419 (95%)
 Frame = -2

Query: 1258 VCEDLTMVSPPISVIQVLNSMADGKKYITSEELQKHNKPGDLWISIQGKVYNVTEWAKEH 1079
            +CED TMVS P+ V++  NS +DGKKYITS+EL+KHNKP DLWISIQGKVYNVTEWAK H
Sbjct: 1    MCEDSTMVSSPVDVLKS-NSKSDGKKYITSDELKKHNKPNDLWISIQGKVYNVTEWAKIH 59

Query: 1078 PGGDIPLLNLAGQDVTDAFIAFHPGTAWKYLDKLFTGYHLKDYQVSDVSKDYRKLASEFA 899
            PGG+IPL+NLAGQDVTDAFIAFHPG+AW++L KLFTGYHLKDYQVS VSKDYRKLASEFA
Sbjct: 60   PGGEIPLMNLAGQDVTDAFIAFHPGSAWEHLSKLFTGYHLKDYQVSAVSKDYRKLASEFA 119

Query: 898  KAGMFEKKGHGVIYSLCFVSLLLSACVYGVLYSSSFWVHMFAGALLGLAWMQIAYLGHDA 719
            KAGMFEKKGHGVIYSLCFVSLLLS CVYGVL+SSSFWVHM AGALLGLAW+QIAYLGHDA
Sbjct: 120  KAGMFEKKGHGVIYSLCFVSLLLSGCVYGVLFSSSFWVHMLAGALLGLAWIQIAYLGHDA 179

Query: 718  GHYQMMATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS 539
            GHYQMM+TRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS
Sbjct: 180  GHYQMMSTRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS 239

Query: 538  SKLFNSLTSVFYGRQLTFDPLARFFVSYQHYSYYPIMCVARVNLYLQTLLLLFSKRKVPD 359
            S+LFNSLTSVFYGRQLTFD LARFFVSYQHYSYYPIMCVARVNLYLQTLLLL SKRKVPD
Sbjct: 240  SRLFNSLTSVFYGRQLTFDRLARFFVSYQHYSYYPIMCVARVNLYLQTLLLLLSKRKVPD 299

Query: 358  RGLNILGTLVFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAADVYVGAP 179
            +GLNI GTLVFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAA+VYVG P
Sbjct: 300  KGLNIFGTLVFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAANVYVGPP 359

Query: 178  KGNDWFEKQTSGTIDIACSSWMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVRELCKK 2
             GNDWFEKQT+GTIDIACSS+MDWFFGGLQFQLEHHLFPRLPRCHLRSI+PIVRELCKK
Sbjct: 360  TGNDWFEKQTNGTIDIACSSYMDWFFGGLQFQLEHHLFPRLPRCHLRSIAPIVRELCKK 418


>sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desaturase; AltName:
            Full=Delta(8)-sphingolipid desaturase
            gi|1040729|emb|CAA60621.1| delta-8 sphingolipid
            desaturase [Helianthus annuus]
          Length = 458

 Score =  811 bits (2094), Expect = 0.0
 Identities = 377/413 (91%), Positives = 396/413 (95%)
 Frame = -2

Query: 1240 MVSPPISVIQVLNSMADGKKYITSEELQKHNKPGDLWISIQGKVYNVTEWAKEHPGGDIP 1061
            MVSP I   +VLNS+ADGKKYITS+EL+KHN P DLWISI GKVYNVTEWAKEHPGGD P
Sbjct: 1    MVSPSI---EVLNSIADGKKYITSKELKKHNNPNDLWISILGKVYNVTEWAKEHPGGDAP 57

Query: 1060 LLNLAGQDVTDAFIAFHPGTAWKYLDKLFTGYHLKDYQVSDVSKDYRKLASEFAKAGMFE 881
            L+NLAGQDVTDAFIAFHPGTAWK+LDKLFTGYHLKDYQVSD+S+DYRKLASEFAKAGMFE
Sbjct: 58   LINLAGQDVTDAFIAFHPGTAWKHLDKLFTGYHLKDYQVSDISRDYRKLASEFAKAGMFE 117

Query: 880  KKGHGVIYSLCFVSLLLSACVYGVLYSSSFWVHMFAGALLGLAWMQIAYLGHDAGHYQMM 701
            KKGHGVIYSLCFVSLLLSACVYGVLYS SFW+HM +GA+LGLAWMQIAYLGHDAGHYQMM
Sbjct: 118  KKGHGVIYSLCFVSLLLSACVYGVLYSGSFWIHMLSGAILGLAWMQIAYLGHDAGHYQMM 177

Query: 700  ATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNS 521
            ATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNS
Sbjct: 178  ATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNS 237

Query: 520  LTSVFYGRQLTFDPLARFFVSYQHYSYYPIMCVARVNLYLQTLLLLFSKRKVPDRGLNIL 341
            +TSVFYGRQLTFDPLARFFVSYQHY YYPIMCVARVNLYLQT+LLL SKRK+PDRGLNIL
Sbjct: 238  ITSVFYGRQLTFDPLARFFVSYQHYLYYPIMCVARVNLYLQTILLLISKRKIPDRGLNIL 297

Query: 340  GTLVFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAADVYVGAPKGNDWF 161
            GTL+FWTWFPLLVS LPNWPERVAFVLVSFCVTGIQH+QFTLNHF+ DVYVG PKG++WF
Sbjct: 298  GTLIFWTWFPLLVSRLPNWPERVAFVLVSFCVTGIQHIQFTLNHFSGDVYVGPPKGDNWF 357

Query: 160  EKQTSGTIDIACSSWMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVRELCKK 2
            EKQT GTIDIACSSWMDWFFGGLQFQLEHHLFPRLPRCHLRSISPI RELCKK
Sbjct: 358  EKQTRGTIDIACSSWMDWFFGGLQFQLEHHLFPRLPRCHLRSISPICRELCKK 410


>gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]
          Length = 469

 Score =  794 bits (2050), Expect = 0.0
 Identities = 368/421 (87%), Positives = 399/421 (94%), Gaps = 2/421 (0%)
 Frame = -2

Query: 1258 VCEDLTMVSPPISVIQVLNSMA--DGKKYITSEELQKHNKPGDLWISIQGKVYNVTEWAK 1085
            +CED TMVSP ++V++VL S +  DGKKYITSEEL+KHNKP DLWISIQGKVYNVTEW+K
Sbjct: 1    MCEDSTMVSPSVAVVEVLKSTSSSDGKKYITSEELEKHNKPTDLWISIQGKVYNVTEWSK 60

Query: 1084 EHPGGDIPLLNLAGQDVTDAFIAFHPGTAWKYLDKLFTGYHLKDYQVSDVSKDYRKLASE 905
             HPGGDIPLLNLAGQDVTDAFIAFHPG+AW +L  LFTGYHL+DYQVSD+SKDYRKLASE
Sbjct: 61   IHPGGDIPLLNLAGQDVTDAFIAFHPGSAWHHLQSLFTGYHLQDYQVSDISKDYRKLASE 120

Query: 904  FAKAGMFEKKGHGVIYSLCFVSLLLSACVYGVLYSSSFWVHMFAGALLGLAWMQIAYLGH 725
            F+KAGMF+KKGHGVIYSLCFVS+LLS CVYGVL S+S  VHMFAGALLGLAWMQIAYLGH
Sbjct: 121  FSKAGMFDKKGHGVIYSLCFVSILLSLCVYGVLCSASVTVHMFAGALLGLAWMQIAYLGH 180

Query: 724  DAGHYQMMATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLA 545
            DAGHYQMMATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLA
Sbjct: 181  DAGHYQMMATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLA 240

Query: 544  VSSKLFNSLTSVFYGRQLTFDPLARFFVSYQHYSYYPIMCVARVNLYLQTLLLLFSKRKV 365
            VSSKLF S+TSVFYGR+LTFD +ARFF+SYQHY+YYPIMCV RVNLYLQT+LLL SKR++
Sbjct: 241  VSSKLFESITSVFYGRRLTFDSVARFFISYQHYTYYPIMCVTRVNLYLQTILLLCSKRRI 300

Query: 364  PDRGLNILGTLVFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAADVYVG 185
            PDR LNI+GTL+FWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAADVYVG
Sbjct: 301  PDRTLNIIGTLIFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAADVYVG 360

Query: 184  APKGNDWFEKQTSGTIDIACSSWMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVRELCK 5
             P+GNDWFEKQT+GTIDI+CSS+MDWFFGGLQFQLEHHLFPRLPRCHLRSISPIV +LCK
Sbjct: 361  PPRGNDWFEKQTAGTIDISCSSYMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVMDLCK 420

Query: 4    K 2
            K
Sbjct: 421  K 421


>ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vinifera]
          Length = 447

 Score =  677 bits (1748), Expect = 0.0
 Identities = 301/399 (75%), Positives = 354/399 (88%)
 Frame = -2

Query: 1198 MADGKKYITSEELQKHNKPGDLWISIQGKVYNVTEWAKEHPGGDIPLLNLAGQDVTDAFI 1019
            MA  +KYI++EEL++H+KPGDLWISIQGKVYNVT+WAK HPGGDIPLLNLAGQDVTDAFI
Sbjct: 1    MAGERKYISAEELKQHDKPGDLWISIQGKVYNVTDWAKVHPGGDIPLLNLAGQDVTDAFI 60

Query: 1018 AFHPGTAWKYLDKLFTGYHLKDYQVSDVSKDYRKLASEFAKAGMFEKKGHGVIYSLCFVS 839
            A+HPGTAW+YLDK FTGY+LKD++VS+VSKD+RKLA+EF+K G+FEKKGHG++Y+   + 
Sbjct: 61   AYHPGTAWQYLDKFFTGYYLKDFKVSEVSKDFRKLANEFSKLGLFEKKGHGILYTCSIIG 120

Query: 838  LLLSACVYGVLYSSSFWVHMFAGALLGLAWMQIAYLGHDAGHYQMMATRGWNKFAGIFIG 659
             L   C+YGVL   SFWVH+   ALLG  W+Q +YLGHD+GHYQ+M++ G+NK A + IG
Sbjct: 121  FLFMVCIYGVLCVDSFWVHLGCAALLGFLWIQSSYLGHDSGHYQIMSSPGFNKLAQMLIG 180

Query: 658  NCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNSLTSVFYGRQLTFDP 479
            NCITGISIAWWKWTHNAHHIACNSLDYDPDLQH+P+ AVSS LF+S+ SVFYGR L FD 
Sbjct: 181  NCITGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAVSSSLFDSINSVFYGRILKFDS 240

Query: 478  LARFFVSYQHYSYYPIMCVARVNLYLQTLLLLFSKRKVPDRGLNILGTLVFWTWFPLLVS 299
            +ARFFVSYQH++YYP+MCVAR+NL+LQTLLLLFS R +PDR LNI+G  VFWTW+PLLVS
Sbjct: 241  VARFFVSYQHWTYYPVMCVARINLFLQTLLLLFSGRNIPDRALNIMGICVFWTWYPLLVS 300

Query: 298  CLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAADVYVGAPKGNDWFEKQTSGTIDIACSS 119
            CLPNW ERV FV+VS  VT IQH+QFTLNHF+A+VYVG P GNDWFEKQTSGTIDI+CS+
Sbjct: 301  CLPNWGERVMFVIVSMAVTSIQHIQFTLNHFSANVYVGPPCGNDWFEKQTSGTIDISCSA 360

Query: 118  WMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVRELCKK 2
            WMDWF GGLQFQLEHHLFPRLPRCHLRSISP+V++LCKK
Sbjct: 361  WMDWFHGGLQFQLEHHLFPRLPRCHLRSISPVVKDLCKK 399


>ref|XP_002302613.1| predicted protein [Populus trichocarpa] gi|222844339|gb|EEE81886.1|
            predicted protein [Populus trichocarpa]
          Length = 447

 Score =  663 bits (1711), Expect = 0.0
 Identities = 295/399 (73%), Positives = 343/399 (85%)
 Frame = -2

Query: 1198 MADGKKYITSEELQKHNKPGDLWISIQGKVYNVTEWAKEHPGGDIPLLNLAGQDVTDAFI 1019
            M   KKYIT EEL++HNK GDLWISIQGKVYNV++WA EHPGGD+ LLNLAGQDVTDAFI
Sbjct: 1    MEGDKKYITGEELKQHNKEGDLWISIQGKVYNVSDWAYEHPGGDVALLNLAGQDVTDAFI 60

Query: 1018 AFHPGTAWKYLDKLFTGYHLKDYQVSDVSKDYRKLASEFAKAGMFEKKGHGVIYSLCFVS 839
            A+HPGTAW+YLDK FTGY+LKD++VS+ SKDYR+LASEFAK G+FEKKGH  +Y+L  ++
Sbjct: 61   AYHPGTAWQYLDKFFTGYYLKDFKVSETSKDYRRLASEFAKLGLFEKKGHITMYALASIA 120

Query: 838  LLLSACVYGVLYSSSFWVHMFAGALLGLAWMQIAYLGHDAGHYQMMATRGWNKFAGIFIG 659
            L+    +YGVL   S W H+ +  +LG  W+Q AY+GHD+GHYQ+M +RG+NKFA    G
Sbjct: 121  LMFCVVLYGVLCCQSVWAHLGSALVLGFLWIQSAYIGHDSGHYQVMKSRGYNKFAQFVAG 180

Query: 658  NCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNSLTSVFYGRQLTFDP 479
            N +TGISIAWWKWTHNAHH+ACNSLDYDPDLQH+P+ AV+S  FNS+ S FYGR L FDP
Sbjct: 181  NSLTGISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVNSTFFNSIKSCFYGRYLDFDP 240

Query: 478  LARFFVSYQHYSYYPIMCVARVNLYLQTLLLLFSKRKVPDRGLNILGTLVFWTWFPLLVS 299
            +ARFFVSYQH+++YP+MCVARVNLYLQT LLLFSKRK PDR LNILG L+FWTWFPLLVS
Sbjct: 241  VARFFVSYQHWTFYPVMCVARVNLYLQTFLLLFSKRKFPDRALNILGILIFWTWFPLLVS 300

Query: 298  CLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAADVYVGAPKGNDWFEKQTSGTIDIACSS 119
            CLPNWPERV FVL SF VT IQHVQF LNHFAADVY G PKGNDWFEKQT GT+DI+CSS
Sbjct: 301  CLPNWPERVMFVLASFAVTAIQHVQFCLNHFAADVYTGPPKGNDWFEKQTGGTLDISCSS 360

Query: 118  WMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVRELCKK 2
            WMDWF+GGLQFQLEHHLFPR+PRC LR +SP+V++LCKK
Sbjct: 361  WMDWFYGGLQFQLEHHLFPRMPRCQLRRVSPLVQDLCKK 399