BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00029515
         (497 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   180   1e-43
ref|XP_002513351.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   178   5e-43
ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222...   166   1e-39
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              166   2e-39
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   166   2e-39

>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223547255|gb|EEF48750.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 496

 Score =  180 bits (456), Expect = 1e-43
 Identities = 91/166 (54%), Positives = 118/166 (71%), Gaps = 3/166 (1%)
 Frame = -1

Query: 494 WPIIGNLHQVGKNPHVSTAILAQEHGPLISLRLGTQLLVVASSPEAATEILKTHDRLLSS 315
           WPI+GN+ QVGKNPH+S A  A+ HGPLISLRLGT+++VVASSP AA EILKTHDRLLS 
Sbjct: 44  WPILGNILQVGKNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSG 103

Query: 314 RFVPNAF---QNYLSPFALIWSSDYNENWRSLRTLCRTEMFSAKALEYQSSLRARKLSQM 144
           R++P       N L   AL+W+   ++ W+ LR +CR+E+FSAKA+E Q++LR +KL++M
Sbjct: 104 RYIPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEM 163

Query: 143 IDFLCSXXXXXXXXXXXVFTTMFNTLSNIFFGKDFLDLKEENGTAG 6
           +DFL S           VFTT FNT+SN+ F KD L   E+ G AG
Sbjct: 164 LDFLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSF-EDQGNAG 208


>ref|XP_002513351.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223547259|gb|EEF48754.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 318

 Score =  178 bits (451), Expect = 5e-43
 Identities = 85/167 (50%), Positives = 119/167 (71%), Gaps = 3/167 (1%)
 Frame = -1

Query: 497 PWPIIGNLHQVGKNPHVSTAILAQEHGPLISLRLGTQLLVVASSPEAATEILKTHDRLLS 318
           PWPIIGNLHQ+GK  H+S    A+ HGPLISLRLG++++VV SSP AATEILKTHDRLLS
Sbjct: 50  PWPIIGNLHQIGKKLHISVTQFAKVHGPLISLRLGSRVVVVGSSPLAATEILKTHDRLLS 109

Query: 317 SRFVPNAFQ---NYLSPFALIWSSDYNENWRSLRTLCRTEMFSAKALEYQSSLRARKLSQ 147
           +R +P       +++   A++W+S  NE W+SLR LCRTE+FSA A+E Q++LR +K+ +
Sbjct: 110 ARTIPKVLPYKIHFVDRLAIVWASSCNERWKSLRALCRTELFSANAIESQAALREKKMIE 169

Query: 146 MIDFLCSXXXXXXXXXXXVFTTMFNTLSNIFFGKDFLDLKEENGTAG 6
           M+++L S           VFT++FNT+SN+ F KD L  +++   +G
Sbjct: 170 MVEYLSSEQGQVVNIGEVVFTSVFNTISNLIFSKDLLSFEDQERGSG 216


>ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222859506|gb|EEE97053.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 503

 Score =  166 bits (421), Expect = 1e-39
 Identities = 86/167 (51%), Positives = 113/167 (67%), Gaps = 3/167 (1%)
 Frame = -1

Query: 497 PWPIIGNLHQVGKNPHVSTAILAQEHGPLISLRLGTQLLVVASSPEAATEILKTHDRLLS 318
           PWPIIGNL  VG  PHVS A +A+ HGPLISLRLGTQLLVV SS +AA EILKTHDR LS
Sbjct: 50  PWPIIGNLLHVGNQPHVSLAEIAKIHGPLISLRLGTQLLVVGSSAKAAAEILKTHDRFLS 109

Query: 317 SRFVPNAF---QNYLSPFALIWSSDYNENWRSLRTLCRTEMFSAKALEYQSSLRARKLSQ 147
           +R VP       + L   AL+W  +  + W+ LR LCRTE+FSAKA+E  ++LR +K+ +
Sbjct: 110 ARHVPQVIPRESHVLRRVALVWCPESIDTWKLLRGLCRTELFSAKAIESSATLREKKVGE 169

Query: 146 MIDFLCSXXXXXXXXXXXVFTTMFNTLSNIFFGKDFLDLKEENGTAG 6
           ++DFL +           VF+T+FNT+SN+ F  D   L+E+  ++G
Sbjct: 170 LMDFLVAREGKVVSIGEVVFSTVFNTISNLLFSNDLAGLEEKGMSSG 216


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  166 bits (420), Expect = 2e-39
 Identities = 89/171 (52%), Positives = 115/171 (67%), Gaps = 6/171 (3%)
 Frame = -1

Query: 497  PWPIIGNLHQVGKNPHVSTAILAQEHGPLISLRLGTQLLVVASSPEAATEILKTHDRLLS 318
            PWPIIGNL Q+GKNPH   A LA+ HGPL+SLRLGTQL+VVASSP AA E+LKTHDR LS
Sbjct: 584  PWPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALS 643

Query: 317  SRF------VPNAFQNYLSPFALIWSSDYNENWRSLRTLCRTEMFSAKALEYQSSLRARK 156
             R+      V N   N+LS   ++++ D N NW++LR +CR E+FS KA+E Q  LR RK
Sbjct: 644  GRYLSSSVPVKNPKLNHLS---IVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERK 700

Query: 155  LSQMIDFLCSXXXXXXXXXXXVFTTMFNTLSNIFFGKDFLDLKEENGTAGG 3
            ++++++FL +           VFTT+ N LSN FF  D  D ++E G  GG
Sbjct: 701  VTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDE-GRVGG 750



 Score =  166 bits (419), Expect = 2e-39
 Identities = 85/162 (52%), Positives = 110/162 (67%), Gaps = 3/162 (1%)
 Frame = -1

Query: 497  PWPIIGNLHQVGKNPHVSTAILAQEHGPLISLRLGTQLLVVASSPEAATEILKTHDRLLS 318
            PWPIIGNL Q+GKNPHV  A LA+ HGPL+SLRLGTQL+VVASSP AA E+LKTHDR LS
Sbjct: 1089 PWPIIGNLLQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLS 1148

Query: 317  SRFVPNAF---QNYLSPFALIWSSDYNENWRSLRTLCRTEMFSAKALEYQSSLRARKLSQ 147
             R+V ++       L+  +L ++ +   NW++LRT+CRTEMFS KA+E    LR RK+ +
Sbjct: 1149 GRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVME 1208

Query: 146  MIDFLCSXXXXXXXXXXXVFTTMFNTLSNIFFGKDFLDLKEE 21
            +++FL +           VFTT+ N LSN FF  D  D + E
Sbjct: 1209 LVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFERE 1250



 Score =  144 bits (364), Expect = 6e-33
 Identities = 77/160 (48%), Positives = 103/160 (64%), Gaps = 3/160 (1%)
 Frame = -1

Query: 497 PWPIIGNLHQVGKNPHVSTAILAQEHGPLISLRLGTQLLVVASSPEAATEILKTHDRLLS 318
           PWPIIGNL Q+G N HV  A LA+ HGPL+SLRLGTQ++VVASS  AA E+LKTHDR LS
Sbjct: 44  PWPIIGNLLQMGGNLHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLS 103

Query: 317 SRFVPNAF---QNYLSPFALIWSSDYNENWRSLRTLCRTEMFSAKALEYQSSLRARKLSQ 147
            R+V          L+  A+ ++   N +WR+L+ +CR E+FS KA+E +  LR RK+ +
Sbjct: 104 GRYVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVME 163

Query: 146 MIDFLCSXXXXXXXXXXXVFTTMFNTLSNIFFGKDFLDLK 27
           +++FL             V+TT+ N LSN FF  DF D +
Sbjct: 164 LVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFE 203


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 498

 Score =  166 bits (420), Expect = 2e-39
 Identities = 89/171 (52%), Positives = 115/171 (67%), Gaps = 6/171 (3%)
 Frame = -1

Query: 497 PWPIIGNLHQVGKNPHVSTAILAQEHGPLISLRLGTQLLVVASSPEAATEILKTHDRLLS 318
           PWPIIGNL Q+GKNPH   A LA+ HGPL+SLRLGTQL+VVASSP AA E+LKTHDR LS
Sbjct: 44  PWPIIGNLLQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALS 103

Query: 317 SRF------VPNAFQNYLSPFALIWSSDYNENWRSLRTLCRTEMFSAKALEYQSSLRARK 156
            R+      V N   N+LS   ++++ D N NW++LR +CR E+FS KA+E Q  LR RK
Sbjct: 104 GRYLSSSVPVKNPKLNHLS---IVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERK 160

Query: 155 LSQMIDFLCSXXXXXXXXXXXVFTTMFNTLSNIFFGKDFLDLKEENGTAGG 3
           ++++++FL +           VFTT+ N LSN FF  D  D ++E G  GG
Sbjct: 161 VTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDE-GRVGG 210