BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00023796
         (1472 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI19780.3| unnamed protein product [Vitis vinifera]              722   0.0  
ref|XP_002280835.1| PREDICTED: peptide transporter PTR5-like [Vi...   722   0.0  
ref|XP_002280138.2| PREDICTED: peptide transporter PTR1-like [Vi...   709   0.0  
ref|XP_002306899.1| predicted protein [Populus trichocarpa] gi|2...   704   0.0  
ref|XP_002510466.1| peptide transporter, putative [Ricinus commu...   699   0.0  

>emb|CBI19780.3| unnamed protein product [Vitis vinifera]
          Length = 1198

 Score =  722 bits (1863), Expect = 0.0
 Identities = 360/489 (73%), Positives = 416/489 (85%)
 Frame = -3

Query: 1467 EEPIPISNMTLVSNGCVNYRGMIADKRKTGGWRASPFIIVNEVAERLAFFAVAVSMVLYL 1288
            +E + +S  TLVSNGCV+ RG IADK++TGGW+ASPFIIVNEVAERLAFFA+AV+MV YL
Sbjct: 27   KEEVGVST-TLVSNGCVDIRGRIADKQRTGGWKASPFIIVNEVAERLAFFAIAVNMVQYL 85

Query: 1287 VREMHQSLPNAATHVTDWIGAAYVLTLVGAFIADAYLGRFLTIIIFSFVYFGGMVMLTIS 1108
            V +M+QSLP AATHVTDWIGAAYVLT++GAF+ADAYLGRF TIIIFS VY  GMV+LT+S
Sbjct: 86   VLQMNQSLPKAATHVTDWIGAAYVLTILGAFLADAYLGRFRTIIIFSSVYTVGMVLLTLS 145

Query: 1107 ARLDSLRPPLCIKKPCPPASDAQTAFLYXXXXXXXXXXXXIKPCVSSFGADQFDEEDDRE 928
            A +DSLRPP C  +PC PAS  QT FLY            IKPCVSSFGADQFDE D  E
Sbjct: 146  ASIDSLRPPQCPTRPCEPASRGQTTFLYGALALIALGTGGIKPCVSSFGADQFDEADGEE 205

Query: 927  VLKKYAFFNWFFFAINMGALLGITLLVYVQQEKGFGWGFGIPTMTMFCSILILLAGFSTY 748
            V KKY+FFNWFFFAINMGALLGIT+LVY+Q EKG+ WGFGIPT  MFCSI+IL AG   Y
Sbjct: 206  VQKKYSFFNWFFFAINMGALLGITILVYIQVEKGWNWGFGIPTAAMFCSIIILAAGIPYY 265

Query: 747  RYKKPMGSAFTRFLQVMVVAVKNHLRGVRVVSDGQLYEVATQESDIFGARKLSHTPQYRF 568
            R++KPMGSAFTRFLQV+V +V+NH +GV      +LYEV T ESDI G RK++HT QYRF
Sbjct: 266  RFQKPMGSAFTRFLQVIVASVRNHFKGVEAGHGAELYEVKTTESDILGNRKIAHTVQYRF 325

Query: 567  LDKAAVIEDHESSTTRNRWKLCTVTQVEEFKCFIRVLPVWATTIALSISFAQLSTFFLSQ 388
            LDKAAVI D E++ T++RW+LCTVTQVEEFK F+R+LPVWA+TIALSISFAQLSTFF+SQ
Sbjct: 326  LDKAAVITDAEAN-TKSRWRLCTVTQVEEFKSFVRILPVWASTIALSISFAQLSTFFISQ 384

Query: 387  AATMDRKLGSNFVIPSGSVPVFSAINALLLVPIYEKVIVPFLRSKTGHQRGITSLQRMGV 208
            A  MDRKLG +FVIP+GS+PVFSA+NAL+LVPIYEK IVP LR  TGH+RGITSLQRMG+
Sbjct: 385  AHIMDRKLGPDFVIPAGSIPVFSAMNALILVPIYEKWIVPILRRHTGHRRGITSLQRMGI 444

Query: 207  GLFISIFAMASAAVVEHKRRNHSNPTTLSVFWLFPQFFLMGSAEVFTYVGQLEFFYDEAT 28
            GLF+SIFA+ASAA+VE +RR+HS  +T+SVFWL PQFFL+GSAEVFTYVGQLEFFYDEAT
Sbjct: 445  GLFVSIFALASAALVEKRRRDHSRASTMSVFWLLPQFFLIGSAEVFTYVGQLEFFYDEAT 504

Query: 27   DGTRSLSSA 1
            DGTRSLSSA
Sbjct: 505  DGTRSLSSA 513



 Score =  709 bits (1830), Expect = 0.0
 Identities = 359/490 (73%), Positives = 408/490 (83%)
 Frame = -3

Query: 1470 EEEPIPISNMTLVSNGCVNYRGMIADKRKTGGWRASPFIIVNEVAERLAFFAVAVSMVLY 1291
            EEE +     TLVSNG V++RG IADKR TGGW+ASPFII NEVAERLAFFA+AV+MV Y
Sbjct: 600  EEESL---RTTLVSNGRVDFRGRIADKRTTGGWKASPFIIANEVAERLAFFAIAVNMVSY 656

Query: 1290 LVREMHQSLPNAATHVTDWIGAAYVLTLVGAFIADAYLGRFLTIIIFSFVYFGGMVMLTI 1111
            LV EMHQS+P AATHVTDWIGAAYVLT++GAF+ADA LGRF TI+IFS +Y  GMV+LT+
Sbjct: 657  LVFEMHQSIPKAATHVTDWIGAAYVLTILGAFLADACLGRFGTIVIFSSIYAVGMVLLTL 716

Query: 1110 SARLDSLRPPLCIKKPCPPASDAQTAFLYXXXXXXXXXXXXIKPCVSSFGADQFDEEDDR 931
            SA +DSL P  C K+PC PAS  QT F              IKPCVSSFGADQFDE D+ 
Sbjct: 717  SASIDSLHPSPCTKQPCKPASYGQTVFFSCALALIALGTGGIKPCVSSFGADQFDEADEN 776

Query: 930  EVLKKYAFFNWFFFAINMGALLGITLLVYVQQEKGFGWGFGIPTMTMFCSILILLAGFST 751
            EV KKYAFFNWFFFAIN+GAL GITLLVY+Q EK + WGFG+PT TMFCSI+IL+AG   
Sbjct: 777  EVHKKYAFFNWFFFAINIGALFGITLLVYIQVEKSWSWGFGVPTATMFCSIIILVAGMRY 836

Query: 750  YRYKKPMGSAFTRFLQVMVVAVKNHLRGVRVVSDGQLYEVATQESDIFGARKLSHTPQYR 571
            YRY++PMGSAFTRFLQV+V +V+NH RGV V  +  LYEV+T+ESDIFGA KL HT QY 
Sbjct: 837  YRYQRPMGSAFTRFLQVIVASVRNHSRGVEVGREDDLYEVSTKESDIFGAVKLPHTAQYS 896

Query: 570  FLDKAAVIEDHESSTTRNRWKLCTVTQVEEFKCFIRVLPVWATTIALSISFAQLSTFFLS 391
            FLDKAAVI D E + T+NRW+LCTVTQVEEFK F+RVLPVWA+TIALSISFAQLSTFF+S
Sbjct: 897  FLDKAAVITDAE-AITKNRWRLCTVTQVEEFKSFLRVLPVWASTIALSISFAQLSTFFIS 955

Query: 390  QAATMDRKLGSNFVIPSGSVPVFSAINALLLVPIYEKVIVPFLRSKTGHQRGITSLQRMG 211
            QA  MDRKLG +FVIP+GS+PVFSAI  L+LVPIYEK IVP LR  TGH+RGITSLQRMG
Sbjct: 956  QALIMDRKLGPSFVIPAGSIPVFSAITGLILVPIYEKWIVPILRRYTGHRRGITSLQRMG 1015

Query: 210  VGLFISIFAMASAAVVEHKRRNHSNPTTLSVFWLFPQFFLMGSAEVFTYVGQLEFFYDEA 31
            VGLF+SI A+ASAA+VE KRR+HS P T+SVFWL PQ+FLMGSAEVFTYVGQLEFFYDEA
Sbjct: 1016 VGLFLSIIALASAALVEKKRRDHSKPLTVSVFWLLPQYFLMGSAEVFTYVGQLEFFYDEA 1075

Query: 30   TDGTRSLSSA 1
            TDGTRS+SSA
Sbjct: 1076 TDGTRSISSA 1085


>ref|XP_002280835.1| PREDICTED: peptide transporter PTR5-like [Vitis vinifera]
          Length = 620

 Score =  722 bits (1863), Expect = 0.0
 Identities = 360/489 (73%), Positives = 416/489 (85%)
 Frame = -3

Query: 1467 EEPIPISNMTLVSNGCVNYRGMIADKRKTGGWRASPFIIVNEVAERLAFFAVAVSMVLYL 1288
            +E + +S  TLVSNGCV+ RG IADK++TGGW+ASPFIIVNEVAERLAFFA+AV+MV YL
Sbjct: 27   KEEVGVST-TLVSNGCVDIRGRIADKQRTGGWKASPFIIVNEVAERLAFFAIAVNMVQYL 85

Query: 1287 VREMHQSLPNAATHVTDWIGAAYVLTLVGAFIADAYLGRFLTIIIFSFVYFGGMVMLTIS 1108
            V +M+QSLP AATHVTDWIGAAYVLT++GAF+ADAYLGRF TIIIFS VY  GMV+LT+S
Sbjct: 86   VLQMNQSLPKAATHVTDWIGAAYVLTILGAFLADAYLGRFRTIIIFSSVYTVGMVLLTLS 145

Query: 1107 ARLDSLRPPLCIKKPCPPASDAQTAFLYXXXXXXXXXXXXIKPCVSSFGADQFDEEDDRE 928
            A +DSLRPP C  +PC PAS  QT FLY            IKPCVSSFGADQFDE D  E
Sbjct: 146  ASIDSLRPPQCPTRPCEPASRGQTTFLYGALALIALGTGGIKPCVSSFGADQFDEADGEE 205

Query: 927  VLKKYAFFNWFFFAINMGALLGITLLVYVQQEKGFGWGFGIPTMTMFCSILILLAGFSTY 748
            V KKY+FFNWFFFAINMGALLGIT+LVY+Q EKG+ WGFGIPT  MFCSI+IL AG   Y
Sbjct: 206  VQKKYSFFNWFFFAINMGALLGITILVYIQVEKGWNWGFGIPTAAMFCSIIILAAGIPYY 265

Query: 747  RYKKPMGSAFTRFLQVMVVAVKNHLRGVRVVSDGQLYEVATQESDIFGARKLSHTPQYRF 568
            R++KPMGSAFTRFLQV+V +V+NH +GV      +LYEV T ESDI G RK++HT QYRF
Sbjct: 266  RFQKPMGSAFTRFLQVIVASVRNHFKGVEAGHGAELYEVKTTESDILGNRKIAHTVQYRF 325

Query: 567  LDKAAVIEDHESSTTRNRWKLCTVTQVEEFKCFIRVLPVWATTIALSISFAQLSTFFLSQ 388
            LDKAAVI D E++ T++RW+LCTVTQVEEFK F+R+LPVWA+TIALSISFAQLSTFF+SQ
Sbjct: 326  LDKAAVITDAEAN-TKSRWRLCTVTQVEEFKSFVRILPVWASTIALSISFAQLSTFFISQ 384

Query: 387  AATMDRKLGSNFVIPSGSVPVFSAINALLLVPIYEKVIVPFLRSKTGHQRGITSLQRMGV 208
            A  MDRKLG +FVIP+GS+PVFSA+NAL+LVPIYEK IVP LR  TGH+RGITSLQRMG+
Sbjct: 385  AHIMDRKLGPDFVIPAGSIPVFSAMNALILVPIYEKWIVPILRRHTGHRRGITSLQRMGI 444

Query: 207  GLFISIFAMASAAVVEHKRRNHSNPTTLSVFWLFPQFFLMGSAEVFTYVGQLEFFYDEAT 28
            GLF+SIFA+ASAA+VE +RR+HS  +T+SVFWL PQFFL+GSAEVFTYVGQLEFFYDEAT
Sbjct: 445  GLFVSIFALASAALVEKRRRDHSRASTMSVFWLLPQFFLIGSAEVFTYVGQLEFFYDEAT 504

Query: 27   DGTRSLSSA 1
            DGTRSLSSA
Sbjct: 505  DGTRSLSSA 513


>ref|XP_002280138.2| PREDICTED: peptide transporter PTR1-like [Vitis vinifera]
          Length = 581

 Score =  709 bits (1829), Expect = 0.0
 Identities = 356/480 (74%), Positives = 404/480 (84%)
 Frame = -3

Query: 1440 TLVSNGCVNYRGMIADKRKTGGWRASPFIIVNEVAERLAFFAVAVSMVLYLVREMHQSLP 1261
            TLVSNG V++RG IADKR TGGW+ASPFII NEVAERLAFFA+AV+MV YLV EMHQS+P
Sbjct: 20   TLVSNGRVDFRGRIADKRTTGGWKASPFIIANEVAERLAFFAIAVNMVSYLVFEMHQSIP 79

Query: 1260 NAATHVTDWIGAAYVLTLVGAFIADAYLGRFLTIIIFSFVYFGGMVMLTISARLDSLRPP 1081
             AATHVTDWIGAAYVLT++GAF+ADA LGRF TI+IFS +Y  GMV+LT+SA +DSL P 
Sbjct: 80   KAATHVTDWIGAAYVLTILGAFLADACLGRFGTIVIFSSIYAVGMVLLTLSASIDSLHPS 139

Query: 1080 LCIKKPCPPASDAQTAFLYXXXXXXXXXXXXIKPCVSSFGADQFDEEDDREVLKKYAFFN 901
             C K+PC PAS  QT F              IKPCVSSFGADQFDE D+ EV KKYAFFN
Sbjct: 140  PCTKQPCKPASYGQTVFFSCALALIALGTGGIKPCVSSFGADQFDEADENEVHKKYAFFN 199

Query: 900  WFFFAINMGALLGITLLVYVQQEKGFGWGFGIPTMTMFCSILILLAGFSTYRYKKPMGSA 721
            WFFFAIN+GAL GITLLVY+Q EK + WGFG+PT TMFCSI+IL+AG   YRY++PMGSA
Sbjct: 200  WFFFAINIGALFGITLLVYIQVEKSWSWGFGVPTATMFCSIIILVAGMRYYRYQRPMGSA 259

Query: 720  FTRFLQVMVVAVKNHLRGVRVVSDGQLYEVATQESDIFGARKLSHTPQYRFLDKAAVIED 541
            FTRFLQV+V +V+NH RGV V  +  LYEV+T+ESDIFGA KL HT QY FLDKAAVI D
Sbjct: 260  FTRFLQVIVASVRNHSRGVEVGREDDLYEVSTKESDIFGAVKLPHTAQYSFLDKAAVITD 319

Query: 540  HESSTTRNRWKLCTVTQVEEFKCFIRVLPVWATTIALSISFAQLSTFFLSQAATMDRKLG 361
             E + T+NRW+LCTVTQVEEFK F+RVLPVWA+TIALSISFAQLSTFF+SQA  MDRKLG
Sbjct: 320  AE-AITKNRWRLCTVTQVEEFKSFLRVLPVWASTIALSISFAQLSTFFISQALIMDRKLG 378

Query: 360  SNFVIPSGSVPVFSAINALLLVPIYEKVIVPFLRSKTGHQRGITSLQRMGVGLFISIFAM 181
             +FVIP+GS+PVFSAI  L+LVPIYEK IVP LR  TGH+RGITSLQRMGVGLF+SI A+
Sbjct: 379  PSFVIPAGSIPVFSAITGLILVPIYEKWIVPILRRYTGHRRGITSLQRMGVGLFLSIIAL 438

Query: 180  ASAAVVEHKRRNHSNPTTLSVFWLFPQFFLMGSAEVFTYVGQLEFFYDEATDGTRSLSSA 1
            ASAA+VE KRR+HS P T+SVFWL PQ+FLMGSAEVFTYVGQLEFFYDEATDGTRS+SSA
Sbjct: 439  ASAALVEKKRRDHSKPLTVSVFWLLPQYFLMGSAEVFTYVGQLEFFYDEATDGTRSISSA 498


>ref|XP_002306899.1| predicted protein [Populus trichocarpa] gi|222856348|gb|EEE93895.1|
            predicted protein [Populus trichocarpa]
          Length = 619

 Score =  704 bits (1818), Expect = 0.0
 Identities = 353/480 (73%), Positives = 404/480 (84%)
 Frame = -3

Query: 1440 TLVSNGCVNYRGMIADKRKTGGWRASPFIIVNEVAERLAFFAVAVSMVLYLVREMHQSLP 1261
            TLVSNGCV+ RG IADKR TGGW+A+PFIIVNEVAERLAF+A+AV+MV YLV +MHQSLP
Sbjct: 41   TLVSNGCVDIRGRIADKRTTGGWKAAPFIIVNEVAERLAFYAIAVNMVAYLVFQMHQSLP 100

Query: 1260 NAATHVTDWIGAAYVLTLVGAFIADAYLGRFLTIIIFSFVYFGGMVMLTISARLDSLRPP 1081
            +AATHVTDWIGAA+VLTL GAF ADAYLGRF TIIIFS +Y  GMV+LT+SA +DSLRPP
Sbjct: 101  DAATHVTDWIGAAFVLTLFGAFCADAYLGRFKTIIIFSCIYAVGMVLLTLSASIDSLRPP 160

Query: 1080 LCIKKPCPPASDAQTAFLYXXXXXXXXXXXXIKPCVSSFGADQFDEEDDREVLKKYAFFN 901
             C  +PCP A+  QT FLY            IKPCVS+FGADQFDE D +EV KKYAFFN
Sbjct: 161  QCKVRPCPKATGGQTWFLYGALALIALGTGGIKPCVSTFGADQFDEADKKEVPKKYAFFN 220

Query: 900  WFFFAINMGALLGITLLVYVQQEKGFGWGFGIPTMTMFCSILILLAGFSTYRYKKPMGSA 721
            WFF AINMGAL GIT+ VY+Q  KG+ WGFG+PT  M  SI+IL+AG   YR+++PMGS 
Sbjct: 221  WFFLAINMGALFGITVFVYIQDNKGWAWGFGLPTGAMVISIVILVAGIRFYRFQEPMGSP 280

Query: 720  FTRFLQVMVVAVKNHLRGVRVVSDGQLYEVATQESDIFGARKLSHTPQYRFLDKAAVIED 541
            FTRF+QVM  +V+NHL GV V    +LYEV T ESDI GA+KLSHT QY FLDKAAV+ D
Sbjct: 281  FTRFVQVMWASVRNHLHGVLVGHQTELYEVNTNESDIKGAQKLSHTIQYSFLDKAAVVTD 340

Query: 540  HESSTTRNRWKLCTVTQVEEFKCFIRVLPVWATTIALSISFAQLSTFFLSQAATMDRKLG 361
             E+  TRNRW+LCTVTQVEEFK FIR+LPVWA+TIALSISFAQLSTFF+SQA  MDRKLG
Sbjct: 341  PEAD-TRNRWRLCTVTQVEEFKSFIRILPVWASTIALSISFAQLSTFFISQANIMDRKLG 399

Query: 360  SNFVIPSGSVPVFSAINALLLVPIYEKVIVPFLRSKTGHQRGITSLQRMGVGLFISIFAM 181
            SNF+IP+GSVP+FS INAL+LVPIYEKVIVP LR +TGH RGITSLQR+GVGLFISIFA+
Sbjct: 400  SNFIIPAGSVPIFSVINALILVPIYEKVIVPILRKRTGHSRGITSLQRIGVGLFISIFAV 459

Query: 180  ASAAVVEHKRRNHSNPTTLSVFWLFPQFFLMGSAEVFTYVGQLEFFYDEATDGTRSLSSA 1
            ASAA+VE KRR+  NP+ +SVFWLFPQFFL+GSAEVF+YVGQLEFFYDEATDGTRS+SSA
Sbjct: 460  ASAALVEKKRRHSPNPSDMSVFWLFPQFFLIGSAEVFSYVGQLEFFYDEATDGTRSISSA 519


>ref|XP_002510466.1| peptide transporter, putative [Ricinus communis]
            gi|223551167|gb|EEF52653.1| peptide transporter, putative
            [Ricinus communis]
          Length = 603

 Score =  699 bits (1803), Expect = 0.0
 Identities = 350/481 (72%), Positives = 406/481 (84%), Gaps = 1/481 (0%)
 Frame = -3

Query: 1440 TLVSNGCVNYRGMIADKRKTGGWRASPFIIVNEVAERLAFFAVAVSMVLYLVREMHQSLP 1261
            TLVSNGCV+ RG IADKR TGGW+ASPFIIVNEVAERLAFFA+AV+MV YL  EM QSLP
Sbjct: 18   TLVSNGCVDIRGRIADKRYTGGWKASPFIIVNEVAERLAFFAIAVNMVAYLAGEMRQSLP 77

Query: 1260 NAATHVTDWIGAAYVLTLVGAFIADAYLGRFLTIIIFSFVYFGGMVMLTISARLDSLRPP 1081
            NA THVTDWIGAAYVLT++GAF+ADAYLGRF TII+FS VY  GMV+LT+SA +DSLRPP
Sbjct: 78   NAITHVTDWIGAAYVLTILGAFLADAYLGRFKTIILFSCVYAVGMVLLTLSASIDSLRPP 137

Query: 1080 LC-IKKPCPPASDAQTAFLYXXXXXXXXXXXXIKPCVSSFGADQFDEEDDREVLKKYAFF 904
             C   + C  A+D Q A+L             IKPCVSSFGADQFDE D++EV KK+AFF
Sbjct: 138  PCKANELCKKATDGQNAYLICALGLIALGTGGIKPCVSSFGADQFDEADEKEVPKKFAFF 197

Query: 903  NWFFFAINMGALLGITLLVYVQQEKGFGWGFGIPTMTMFCSILILLAGFSTYRYKKPMGS 724
            NWFFFAINMGALLGITL+VY+++EKG+GWGFG+PT  M  SI IL AG   YR++KPMGS
Sbjct: 198  NWFFFAINMGALLGITLMVYIKEEKGWGWGFGVPTGAMIVSIFILAAGVPYYRFQKPMGS 257

Query: 723  AFTRFLQVMVVAVKNHLRGVRVVSDGQLYEVATQESDIFGARKLSHTPQYRFLDKAAVIE 544
             FTRFLQVMV ++ NH RGV+V S+ QLYEV +++SDI GARKL HT Q++FLDKAAV+ 
Sbjct: 258  PFTRFLQVMVASLTNHFRGVKVGSEVQLYEVQSEKSDIEGARKLCHTRQFKFLDKAAVVT 317

Query: 543  DHESSTTRNRWKLCTVTQVEEFKCFIRVLPVWATTIALSISFAQLSTFFLSQAATMDRKL 364
            D E++T  NRWKLCTVTQVEEFKC IR+LPVWA+TIALSISF QL+TFF SQA  MDRK 
Sbjct: 318  DPETNTM-NRWKLCTVTQVEEFKCLIRILPVWASTIALSISFTQLATFFTSQALIMDRKF 376

Query: 363  GSNFVIPSGSVPVFSAINALLLVPIYEKVIVPFLRSKTGHQRGITSLQRMGVGLFISIFA 184
            GSNFVIP+GSV VFSAINAL+LVPIYEK +VP +R  T H+RGITSLQRMGVGLF+SIFA
Sbjct: 377  GSNFVIPAGSVTVFSAINALILVPIYEKYVVPIIRKYTNHRRGITSLQRMGVGLFVSIFA 436

Query: 183  MASAAVVEHKRRNHSNPTTLSVFWLFPQFFLMGSAEVFTYVGQLEFFYDEATDGTRSLSS 4
            +ASAA+VE KRR+ S+P+ +SVFWLFPQ+FL+G+AEVFTYVGQLEFFYDEATDGTRS+SS
Sbjct: 437  LASAALVEKKRRDSSSPSDMSVFWLFPQYFLVGTAEVFTYVGQLEFFYDEATDGTRSISS 496

Query: 3    A 1
            A
Sbjct: 497  A 497