BLASTX nr result
ID: Atractylodes21_contig00023405
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes21_contig00023405
(1109 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAD66636.1| ARGONAUTE9 protein [Arabidopsis thaliana] 90 4e-55
gb|AFV15382.1| AGO4B [Solanum lycopersicum] 86 1e-52
ref|XP_003545462.1| PREDICTED: protein argonaute 4-like [Glycine... 93 2e-51
ref|XP_002308843.1| argonaute protein group [Populus trichocarpa... 86 2e-50
ref|XP_002275928.1| PREDICTED: protein argonaute 4 [Vitis vinife... 103 2e-41
>emb|CAD66636.1| ARGONAUTE9 protein [Arabidopsis thaliana]
Length = 896
Score = 89.7 bits (221), Expect(5) = 4e-55
Identities = 43/66 (65%), Positives = 54/66 (81%)
Frame = -1
Query: 539 NFHQSTASQKPENIIISRDGVCESQFNKVLNIELE*IMEVCKFLDDA*DPKFLVIVA*ET 360
+F+ S+ ++KPE+III RDGV ESQFN+VLNIEL+ +M+ CKFLDD PKF VIVA +
Sbjct: 696 DFYYSSENRKPEHIIIFRDGVSESQFNQVLNIELDQMMQACKFLDDTWHPKFTVIVAQKN 755
Query: 359 HHTKFF 342
HHTKFF
Sbjct: 756 HHTKFF 761
Score = 83.2 bits (204), Expect(5) = 4e-55
Identities = 47/95 (49%), Positives = 60/95 (63%)
Frame = -2
Query: 823 INAKMGGINPLISIEHSHCIPVVSRTPTIIFGMDVFYGSPG*SDVPSIAAXXXXXXXXXX 644
INAK+GG+N L+++E S +P V++ PTII GMDV +GSPG SD+PSIAA
Sbjct: 599 INAKLGGLNSLLAMERSPAMPKVTQVPTIIVGMDVSHGSPGQSDIPSIAAVVSSRQWPLI 658
Query: 643 XXXXXXXRMQSARIEMIDNLFNHVSPAKDERMIME 539
R QS ++EMIDNLF V+ KDE M E
Sbjct: 659 SKYKACVRTQSRKMEMIDNLFKPVN-GKDEGMFRE 692
Score = 59.7 bits (143), Expect(5) = 4e-55
Identities = 24/38 (63%), Positives = 31/38 (81%)
Frame = -3
Query: 255 NKVCHPKNNDFCLCAQIGLTGTTRPTHYYVLLD*IGFS 142
+++CHP+N DF LCA G+ GTTRPTHY+VL D IGF+
Sbjct: 777 SQICHPRNFDFYLCAHAGMIGTTRPTHYHVLYDEIGFA 814
Score = 35.8 bits (81), Expect(5) = 4e-55
Identities = 16/22 (72%), Positives = 20/22 (90%)
Frame = -2
Query: 148 FLSDGLQQLVYSLSYVYQRSTT 83
F +D LQ+LV+SLS+VYQRSTT
Sbjct: 813 FATDDLQELVHSLSHVYQRSTT 834
Score = 35.8 bits (81), Expect(5) = 4e-55
Identities = 14/26 (53%), Positives = 21/26 (80%)
Frame = -1
Query: 80 AISIVAPISYAHVAATQMAEFVKFDD 3
AIS+VAP+ YAH+AA QM +K+++
Sbjct: 835 AISVVAPVCYAHLAAAQMGTVMKYEE 860
>gb|AFV15382.1| AGO4B [Solanum lycopersicum]
Length = 913
Score = 85.9 bits (211), Expect(4) = 1e-52
Identities = 39/66 (59%), Positives = 53/66 (80%)
Frame = -1
Query: 539 NFHQSTASQKPENIIISRDGVCESQFNKVLNIELE*IMEVCKFLDDA*DPKFLVIVA*ET 360
+F+ S+ +KPE+II+ RDGV ESQFN+VLNIEL+ ++E C FLD+ PKF++IVA +
Sbjct: 713 DFYVSSGKRKPEHIIVFRDGVSESQFNQVLNIELDQLIEACNFLDEKWSPKFVIIVAQKN 772
Query: 359 HHTKFF 342
HHTKFF
Sbjct: 773 HHTKFF 778
Score = 84.7 bits (208), Expect(4) = 1e-52
Identities = 45/95 (47%), Positives = 60/95 (63%)
Frame = -2
Query: 823 INAKMGGINPLISIEHSHCIPVVSRTPTIIFGMDVFYGSPG*SDVPSIAAXXXXXXXXXX 644
INAK+GG+N +++ E S IP+VS+ PT+I GMDV +GSPG SDVPSIAA
Sbjct: 615 INAKLGGLNSMLAAEISPSIPMVSKVPTMILGMDVSHGSPGQSDVPSIAAVVSSRQWPSI 674
Query: 643 XXXXXXXRMQSARIEMIDNLFNHVSPAKDERMIME 539
R QS ++EMIDN+F VS D+ ++ E
Sbjct: 675 SRYRASVRTQSPKVEMIDNIFKKVSDTDDDGIMRE 709
Score = 69.7 bits (169), Expect(4) = 1e-52
Identities = 29/38 (76%), Positives = 33/38 (86%)
Frame = -3
Query: 255 NKVCHPKNNDFCLCAQIGLTGTTRPTHYYVLLD*IGFS 142
NKVCHP+NNDF LCA G+ GTTRPTHY+VLLD +GFS
Sbjct: 794 NKVCHPRNNDFYLCAHAGMIGTTRPTHYHVLLDEVGFS 831
Score = 35.0 bits (79), Expect(4) = 1e-52
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = -2
Query: 148 FLSDGLQQLVYSLSYVYQRSTT 83
F D LQ+LV++LSYVYQRSTT
Sbjct: 830 FSPDELQELVHNLSYVYQRSTT 851
>ref|XP_003545462.1| PREDICTED: protein argonaute 4-like [Glycine max]
Length = 906
Score = 93.2 bits (230), Expect(3) = 2e-51
Identities = 48/95 (50%), Positives = 62/95 (65%)
Frame = -2
Query: 823 INAKMGGINPLISIEHSHCIPVVSRTPTIIFGMDVFYGSPG*SDVPSIAAXXXXXXXXXX 644
INAK+GG+N ++ +EHS IP+VSR PTII GMDV +GSPG +D+PSIAA
Sbjct: 609 INAKLGGLNSMLGVEHSPSIPIVSRAPTIIIGMDVSHGSPGQTDIPSIAAVVSSREWPLI 668
Query: 643 XXXXXXXRMQSARIEMIDNLFNHVSPAKDERMIME 539
R QS ++EMIDNLF VS +DE ++ E
Sbjct: 669 SKYRASVRTQSPKMEMIDNLFKKVSDKEDEGIMRE 703
Score = 92.4 bits (228), Expect(3) = 2e-51
Identities = 44/66 (66%), Positives = 56/66 (84%)
Frame = -1
Query: 539 NFHQSTASQKPENIIISRDGVCESQFNKVLNIELE*IMEVCKFLDDA*DPKFLVIVA*ET 360
+F+ S+ ++KP+NIII RDGV ESQFN+VLNIEL+ I+E CKFLD+ +PKFLVIVA +
Sbjct: 707 DFYTSSGNRKPDNIIIFRDGVSESQFNQVLNIELDQIIEACKFLDEKWNPKFLVIVAQKN 766
Query: 359 HHTKFF 342
HHTKFF
Sbjct: 767 HHTKFF 772
Score = 65.9 bits (159), Expect(3) = 2e-51
Identities = 34/76 (44%), Positives = 46/76 (60%), Gaps = 8/76 (10%)
Frame = -3
Query: 255 NKVCHPKNNDFCLCAQIGLTGTTRPTHYYVLLD*IGFS----QMACNSLFIHYPMCTKG- 91
NK+CHP+N DF +CA G+ GT+RPTHY+VLLD IGFS Q +SL Y T
Sbjct: 788 NKICHPRNYDFYMCAHAGMIGTSRPTHYHVLLDEIGFSPDDLQELVHSLSYVYQRSTTAI 847
Query: 90 ---AQLCHIYSCSDQL 52
A +C+ + + Q+
Sbjct: 848 SVVAPICYAHLAATQM 863
>ref|XP_002308843.1| argonaute protein group [Populus trichocarpa]
gi|222854819|gb|EEE92366.1| argonaute protein group
[Populus trichocarpa]
Length = 930
Score = 85.9 bits (211), Expect(4) = 2e-50
Identities = 41/66 (62%), Positives = 52/66 (78%)
Frame = -1
Query: 539 NFHQSTASQKPENIIISRDGVCESQFNKVLNIELE*IMEVCKFLDDA*DPKFLVIVA*ET 360
++++S+ KP III RDGV ESQFN+VLNIEL+ I+E CKFLD++ PKF VIVA +
Sbjct: 730 DYYRSSGQTKPAQIIIFRDGVSESQFNQVLNIELDQIIEACKFLDESWSPKFTVIVAQKN 789
Query: 359 HHTKFF 342
HHTKFF
Sbjct: 790 HHTKFF 795
Score = 84.0 bits (206), Expect(4) = 2e-50
Identities = 45/94 (47%), Positives = 59/94 (62%)
Frame = -2
Query: 823 INAKMGGINPLISIEHSHCIPVVSRTPTIIFGMDVFYGSPG*SDVPSIAAXXXXXXXXXX 644
INAK+GG+N L+++E S IP VS+ PTIIFGMDV +GSPG SD+PSIAA
Sbjct: 631 INAKLGGLNSLLAMEQSRNIPFVSKVPTIIFGMDVSHGSPGQSDMPSIAAVVSSRNWPLL 690
Query: 643 XXXXXXXRMQSARIEMIDNLFNHVSPAKDERMIM 542
R QS ++EM+D+LF KD+ I+
Sbjct: 691 SRYRASVRSQSPKVEMVDSLFTLTPDKKDDSGIV 724
Score = 61.6 bits (148), Expect(4) = 2e-50
Identities = 26/38 (68%), Positives = 31/38 (81%)
Frame = -3
Query: 255 NKVCHPKNNDFCLCAQIGLTGTTRPTHYYVLLD*IGFS 142
N VCHP++ DF +CA G+ GTTRPTHY+VLLD IGFS
Sbjct: 811 NAVCHPQSYDFYMCAHAGMIGTTRPTHYHVLLDEIGFS 848
Score = 37.0 bits (84), Expect(4) = 2e-50
Identities = 16/22 (72%), Positives = 20/22 (90%)
Frame = -2
Query: 148 FLSDGLQQLVYSLSYVYQRSTT 83
F +D LQ+L++SLSYVYQRSTT
Sbjct: 847 FSADDLQELIHSLSYVYQRSTT 868
>ref|XP_002275928.1| PREDICTED: protein argonaute 4 [Vitis vinifera]
gi|296083994|emb|CBI24382.3| unnamed protein product
[Vitis vinifera]
Length = 913
Score = 103 bits (258), Expect(2) = 2e-41
Identities = 70/188 (37%), Positives = 101/188 (53%), Gaps = 9/188 (4%)
Frame = -1
Query: 539 NFHQSTASQKPENIIISRDGVCESQFNKVLNIELE*IMEVCKFLDDA*DPKFLVIVA*ET 360
+F+ S+ +KP+ III RDGV ESQFN+VLNIEL+ I+E CKFLD+ PKF+VIVA +
Sbjct: 713 DFYVSSGKRKPDQIIIFRDGVSESQFNQVLNIELDQIIEACKFLDEKWSPKFVVIVAQKN 772
Query: 359 HHTKFFL------LEPILIWKTKISIN*ISLFSCFRENRHTIKFVIRKTMTFACVLKLD* 198
HHTKFF + P + K+ C N + C
Sbjct: 773 HHTKFFQHGSPDNVPPGTVIDNKV---------CHPRNND----------FYLCAH---- 809
Query: 197 LGLHDLPITMYYSIRLVSLRWLA---TACLFTILCVPKEHNFAISIVAPISYAHVAATQM 27
G+ +Y + L + + + + ++ V + AIS+VAPI YAH+AATQM
Sbjct: 810 AGMIGTTRPTHYHVLLDEVGFSSDDLQELVHSLSYVYQRSTTAISVVAPICYAHLAATQM 869
Query: 26 AEFVKFDD 3
++F+KF+D
Sbjct: 870 SQFMKFED 877
Score = 92.8 bits (229), Expect(2) = 2e-41
Identities = 48/95 (50%), Positives = 63/95 (66%)
Frame = -2
Query: 823 INAKMGGINPLISIEHSHCIPVVSRTPTIIFGMDVFYGSPG*SDVPSIAAXXXXXXXXXX 644
INAK+GG+N ++++EHS IP+VS+ PTII GMDV +GSPG SDVPSIAA
Sbjct: 615 INAKLGGLNSMLAVEHSPSIPIVSKGPTIILGMDVSHGSPGQSDVPSIAAVVSSRQWPLI 674
Query: 643 XXXXXXXRMQSARIEMIDNLFNHVSPAKDERMIME 539
R QS ++EMID+L+ VS +DE +I E
Sbjct: 675 SRYRASVRTQSPKVEMIDSLYKRVSETEDEGIIRE 709