BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00018277
         (1768 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAG71197.1| ent-kaurene oxidase 1 [Lactuca sativa]                811   0.0  
gb|AAQ63464.1| ent-kaurene oxidase CYP701A5 [Stevia rebaudiana] ...   728   0.0  
gb|AAY42951.1| kaurene oxidase [Stevia rebaudiana]                    725   0.0  
dbj|BAG71198.1| ent-kaurene oxidase 2 [Lactuca sativa]                709   0.0  
gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera]                   616   e-174

>dbj|BAG71197.1| ent-kaurene oxidase 1 [Lactuca sativa]
          Length = 511

 Score =  811 bits (2094), Expect = 0.0
 Identities = 396/485 (81%), Positives = 436/485 (89%)
 Frame = +3

Query: 123  LSFLILKSFLSHHRRNPNHLPTVPAVPGVPLLGNLLQLKEKKPYKTFTKWAETYGPIYSI 302
            +S L LKSFLS    NPNHLP+VPAVPGVPLLGNLL+LKEKKPYKTFTKWAETYGPIYSI
Sbjct: 27   ISLLFLKSFLSQQPGNPNHLPSVPAVPGVPLLGNLLELKEKKPYKTFTKWAETYGPIYSI 86

Query: 303  KAGATSMVVVSSNHLAKEAMVTRFDSISTRKLSKALRILTADKTMVAMSDYDDYHKTVKR 482
            K GATSMVVV+SN LAKEAMVTRFDSISTRKLSKAL+ILTADKTMVAMSDYDDYHKTVKR
Sbjct: 87   KTGATSMVVVNSNQLAKEAMVTRFDSISTRKLSKALQILTADKTMVAMSDYDDYHKTVKR 146

Query: 483  NILTSVLGPAARKKHRAHRDTMADNVSNQLHALALTSHREAINLREILQSELFTLAFKQV 662
            N+LTS+LGPAA+K+HRAHRD M DN+S QLHALAL S +EAIN R+I QSELFTLAFKQ 
Sbjct: 147  NLLTSILGPAAQKRHRAHRDAMGDNLSRQLHALALNSPQEAINFRQIFQSELFTLAFKQT 206

Query: 663  MGKDFESIYVGDLGITMTKEEVFQVLVIDPMMGAIDVDWRDFFPYLRWIPNTSFEKKIEQ 842
             G+D ESI+VGDLG TMT+EE+FQ+LV+DPMMGAIDVDWRDFFPYL+WIPN   E+KIEQ
Sbjct: 207  FGRDIESIFVGDLGTTMTREEMFQILVVDPMMGAIDVDWRDFFPYLKWIPNAKLEEKIEQ 266

Query: 843  MDIRREAVMKTLIQEHRKRIDSGETLNSYIDYLLSEAQPLTEKQLLMSLWEPIIETSDTT 1022
            M IRR+AVMK +IQEHRKRIDSGE L+SYID+LL+EAQPLTEKQLLMSLWEPIIETSDTT
Sbjct: 267  MYIRRKAVMKAVIQEHRKRIDSGENLDSYIDFLLAEAQPLTEKQLLMSLWEPIIETSDTT 326

Query: 1023 MVTTEWAMYELAKNPKKQDRLYDEIRNVCGSQKITEEKLCQMPYLSAIFHETLRRHSPVP 1202
            MVTTEWAMYEL+K+P KQ RLY+EIRN+CGS+KITEEKLC+MPYLSA+FHETLR HSPV 
Sbjct: 327  MVTTEWAMYELSKHPNKQQRLYNEIRNICGSEKITEEKLCKMPYLSAVFHETLRVHSPVS 386

Query: 1203 IIPLRYVHENTVLGGYHVPAGTELAINIYGCNMXXXXXXXXXXXXXXRFLAENEPIDLQR 1382
            IIPLRYVHENT LGGYHVPAGTELA+NIYGCNM              RFLAENEP++LQ+
Sbjct: 387  IIPLRYVHENTELGGYHVPAGTELAVNIYGCNMEREIWENPEEWSPERFLAENEPVNLQK 446

Query: 1383 TMAFGGGKRVCAGAMQAMLIACVCMGRMVQEFEWRLKDDTGEDVSTIGLTTQMLNPMLAV 1562
            TMAFG GKRVCAGAMQAML+ACV +GRMVQEFEWRLKDD  EDV+T+GLTTQ LNPMLAV
Sbjct: 447  TMAFGAGKRVCAGAMQAMLLACVGIGRMVQEFEWRLKDDVEEDVNTLGLTTQRLNPMLAV 506

Query: 1563 IKPRN 1577
            IKPRN
Sbjct: 507  IKPRN 511


>gb|AAQ63464.1| ent-kaurene oxidase CYP701A5 [Stevia rebaudiana]
            gi|76446107|gb|ABA42921.1| kaurene oxidase [Stevia
            rebaudiana]
          Length = 513

 Score =  728 bits (1878), Expect = 0.0
 Identities = 358/485 (73%), Positives = 411/485 (84%), Gaps = 1/485 (0%)
 Frame = +3

Query: 123  LSFLILKSFLSHHRRNPNHLPTVPAVPGVPLLGNLLQLKEKKPYKTFTKWAETYGPIYSI 302
            L F  LKS+ S  R   NHLP VP VPGVPLLGNLLQLKEKKPY TFT+WA TYGPIYSI
Sbjct: 28   LIFWYLKSYTSARRSQSNHLPRVPEVPGVPLLGNLLQLKEKKPYMTFTRWAATYGPIYSI 87

Query: 303  KAGATSMVVVSSNHLAKEAMVTRFDSISTRKLSKALRILTADKTMVAMSDYDDYHKTVKR 482
            K GATSMVVVSSN +AKEA+VTRF SISTR LSKAL++LTADKTMVAMSDYDDYHKTVKR
Sbjct: 88   KTGATSMVVVSSNEIAKEALVTRFQSISTRNLSKALKVLTADKTMVAMSDYDDYHKTVKR 147

Query: 483  NILTSVLGPAARKKHRAHRDTMADNVSNQLHALALTS-HREAINLREILQSELFTLAFKQ 659
            +ILT+VLGP A+KKHR HRD M DN+S QLH     +  +E ++LR+I QSELF LA +Q
Sbjct: 148  HILTAVLGPNAQKKHRIHRDIMMDNISTQLHEFVKNNPEQEEVDLRKIFQSELFGLAMRQ 207

Query: 660  VMGKDFESIYVGDLGITMTKEEVFQVLVIDPMMGAIDVDWRDFFPYLRWIPNTSFEKKIE 839
             +GKD ES+YV DL ITM ++E+FQVLV+DPMMGAIDVDWRDFFPYL+W+PN  FE  I+
Sbjct: 208  ALGKDVESLYVEDLKITMNRDEIFQVLVVDPMMGAIDVDWRDFFPYLKWVPNKKFENTIQ 267

Query: 840  QMDIRREAVMKTLIQEHRKRIDSGETLNSYIDYLLSEAQPLTEKQLLMSLWEPIIETSDT 1019
            QM IRREAVMK+LI+EH+KRI SGE LNSYIDYLLSEAQ LT++QLLMSLWEPIIE+SDT
Sbjct: 268  QMYIRREAVMKSLIKEHKKRIASGEKLNSYIDYLLSEAQTLTDQQLLMSLWEPIIESSDT 327

Query: 1020 TMVTTEWAMYELAKNPKKQDRLYDEIRNVCGSQKITEEKLCQMPYLSAIFHETLRRHSPV 1199
            TMVTTEWAMYELAKNPK QDRLY +I++VCGS+KITEE L Q+PY++AIFHETLRRHSPV
Sbjct: 328  TMVTTEWAMYELAKNPKLQDRLYRDIKSVCGSEKITEEHLSQLPYITAIFHETLRRHSPV 387

Query: 1200 PIIPLRYVHENTVLGGYHVPAGTELAINIYGCNMXXXXXXXXXXXXXXRFLAENEPIDLQ 1379
            PIIPLR+VHE+TVLGGYHVPAGTELA+NIYGCNM              RF+ ENE ID Q
Sbjct: 388  PIIPLRHVHEDTVLGGYHVPAGTELAVNIYGCNMDKNVWENPEEWNPERFMKENETIDFQ 447

Query: 1380 RTMAFGGGKRVCAGAMQAMLIACVCMGRMVQEFEWRLKDDTGEDVSTIGLTTQMLNPMLA 1559
            +TMAFGGGKRVCAG++QA+L A + +GRMVQEFEW+LKD T E+V+TIGLTTQML P+ A
Sbjct: 448  KTMAFGGGKRVCAGSLQALLTASIGIGRMVQEFEWKLKDMTQEEVNTIGLTTQMLRPLRA 507

Query: 1560 VIKPR 1574
            +IKPR
Sbjct: 508  IIKPR 512


>gb|AAY42951.1| kaurene oxidase [Stevia rebaudiana]
          Length = 513

 Score =  725 bits (1871), Expect = 0.0
 Identities = 357/485 (73%), Positives = 411/485 (84%), Gaps = 1/485 (0%)
 Frame = +3

Query: 123  LSFLILKSFLSHHRRNPNHLPTVPAVPGVPLLGNLLQLKEKKPYKTFTKWAETYGPIYSI 302
            L F  LKS+ S  R   NHLP VP VPGVPLLGNLLQLKEKKPY TFT+WA TYGPIYSI
Sbjct: 28   LIFWYLKSYTSARRSQSNHLPRVPEVPGVPLLGNLLQLKEKKPYMTFTRWAATYGPIYSI 87

Query: 303  KAGATSMVVVSSNHLAKEAMVTRFDSISTRKLSKALRILTADKTMVAMSDYDDYHKTVKR 482
            K GATSMVVVSSN +AKEA+VTRF SISTR LSKAL++LTADKTMVAMSDYDDYHKTVKR
Sbjct: 88   KTGATSMVVVSSNEIAKEALVTRFQSISTRNLSKALKVLTADKTMVAMSDYDDYHKTVKR 147

Query: 483  NILTSVLGPAARKKHRAHRDTMADNVSNQLHALALTS-HREAINLREILQSELFTLAFKQ 659
            +ILT+VLGP A+KKHR HRD M DN+S QLH     +  +E ++LR+I QSELF LA +Q
Sbjct: 148  HILTAVLGPNAQKKHRIHRDIMMDNISTQLHEFVKNNPEQEEVDLRKIFQSELFGLAMRQ 207

Query: 660  VMGKDFESIYVGDLGITMTKEEVFQVLVIDPMMGAIDVDWRDFFPYLRWIPNTSFEKKIE 839
             +GKD ES+YV DL ITM ++E+FQVLV+DPMMGAIDVDWRDFFPYL+W+PN  FE  I+
Sbjct: 208  ALGKDVESLYVEDLKITMNRDEIFQVLVVDPMMGAIDVDWRDFFPYLKWVPNKKFENTIQ 267

Query: 840  QMDIRREAVMKTLIQEHRKRIDSGETLNSYIDYLLSEAQPLTEKQLLMSLWEPIIETSDT 1019
            QM IRREAVMK+LI+E++KRI SGE LNSYIDYLLSEAQ LT++QLLMSLWEPIIE+SDT
Sbjct: 268  QMYIRREAVMKSLIKENKKRIASGEKLNSYIDYLLSEAQTLTDQQLLMSLWEPIIESSDT 327

Query: 1020 TMVTTEWAMYELAKNPKKQDRLYDEIRNVCGSQKITEEKLCQMPYLSAIFHETLRRHSPV 1199
            TMVTTEWAMYELAKNPK QDRLY +I++VCGS+KITEE L Q+PY++AIFHETLRRHSPV
Sbjct: 328  TMVTTEWAMYELAKNPKLQDRLYRDIKSVCGSEKITEEHLSQLPYITAIFHETLRRHSPV 387

Query: 1200 PIIPLRYVHENTVLGGYHVPAGTELAINIYGCNMXXXXXXXXXXXXXXRFLAENEPIDLQ 1379
            PIIPLR+VHE+TVLGGYHVPAGTELA+NIYGCNM              RF+ ENE ID Q
Sbjct: 388  PIIPLRHVHEDTVLGGYHVPAGTELAVNIYGCNMDKNVWENPEEWNPERFMKENETIDFQ 447

Query: 1380 RTMAFGGGKRVCAGAMQAMLIACVCMGRMVQEFEWRLKDDTGEDVSTIGLTTQMLNPMLA 1559
            +TMAFGGGKRVCAG++QA+L A + +GRMVQEFEW+LKD T E+V+TIGLTTQML P+ A
Sbjct: 448  KTMAFGGGKRVCAGSLQALLTASIGIGRMVQEFEWKLKDMTQEEVNTIGLTTQMLRPLRA 507

Query: 1560 VIKPR 1574
            +IKPR
Sbjct: 508  IIKPR 512


>dbj|BAG71198.1| ent-kaurene oxidase 2 [Lactuca sativa]
          Length = 512

 Score =  709 bits (1830), Expect = 0.0
 Identities = 350/486 (72%), Positives = 407/486 (83%), Gaps = 2/486 (0%)
 Frame = +3

Query: 123  LSFLILKSFLS--HHRRNPNHLPTVPAVPGVPLLGNLLQLKEKKPYKTFTKWAETYGPIY 296
            L F  L+S+ S  HH    NHLP VP VPGVP+LGNLLQLKEKKPY TFTKWAE YGPIY
Sbjct: 29   LYFWFLRSYASPSHHS---NHLPPVPEVPGVPVLGNLLQLKEKKPYMTFTKWAEMYGPIY 85

Query: 297  SIKAGATSMVVVSSNHLAKEAMVTRFDSISTRKLSKALRILTADKTMVAMSDYDDYHKTV 476
            SI+ GATSMVVVSSN +AKE +VTRF SISTRKLS AL++LT DK+MVAMSDY DYHKTV
Sbjct: 86   SIRTGATSMVVVSSNEIAKEVVVTRFPSISTRKLSYALKVLTEDKSMVAMSDYHDYHKTV 145

Query: 477  KRNILTSVLGPAARKKHRAHRDTMADNVSNQLHALALTSHREAINLREILQSELFTLAFK 656
            KR+ILT+VLGP A+KK RAHRDTM +NVSN+LHA    +  + +NLR+I QS+LF LA K
Sbjct: 146  KRHILTAVLGPNAQKKFRAHRDTMMENVSNELHAFFEKNPNQEVNLRKIFQSQLFGLAMK 205

Query: 657  QVMGKDFESIYVGDLGITMTKEEVFQVLVIDPMMGAIDVDWRDFFPYLRWIPNTSFEKKI 836
            Q +GKD ESIYV DL  TM +EE+F+VLV+DPMMGAI+VDWRDFFPYL+W+PN SFE  I
Sbjct: 206  QALGKDVESIYVKDLETTMKREEIFEVLVVDPMMGAIEVDWRDFFPYLKWVPNKSFENII 265

Query: 837  EQMDIRREAVMKTLIQEHRKRIDSGETLNSYIDYLLSEAQPLTEKQLLMSLWEPIIETSD 1016
             +M  RREAVMK LIQEH+KRI SGE LNSYIDYLLSEAQ LT+KQLLMSLWEPIIE+SD
Sbjct: 266  HRMYTRREAVMKALIQEHKKRIASGENLNSYIDYLLSEAQTLTDKQLLMSLWEPIIESSD 325

Query: 1017 TTMVTTEWAMYELAKNPKKQDRLYDEIRNVCGSQKITEEKLCQMPYLSAIFHETLRRHSP 1196
            TTMVTTEWAMYELAKNP  QDRLY+EI++VCGS+KITEE L Q+PYL A+F ETLR+H P
Sbjct: 326  TTMVTTEWAMYELAKNPNMQDRLYEEIQSVCGSEKITEENLSQLPYLYAVFQETLRKHCP 385

Query: 1197 VPIIPLRYVHENTVLGGYHVPAGTELAINIYGCNMXXXXXXXXXXXXXXRFLAENEPIDL 1376
            VPI+PLRYVHENTVLGGYHVPAGTE+AINIYGCNM              RFL+E E +DL
Sbjct: 386  VPIMPLRYVHENTVLGGYHVPAGTEVAINIYGCNMDKKVWENPEEWNPERFLSEKESMDL 445

Query: 1377 QRTMAFGGGKRVCAGAMQAMLIACVCMGRMVQEFEWRLKDDTGEDVSTIGLTTQMLNPML 1556
             +TMAFGGGKRVCAG++QAM+I+C+ +GR+VQ+FEW+LKDD  EDV+T+GLTTQ L+P+L
Sbjct: 446  YKTMAFGGGKRVCAGSLQAMVISCIGIGRLVQDFEWKLKDDAEEDVNTLGLTTQKLHPLL 505

Query: 1557 AVIKPR 1574
            A+I PR
Sbjct: 506  ALINPR 511


>gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera]
          Length = 508

 Score =  616 bits (1589), Expect = e-174
 Identities = 297/482 (61%), Positives = 379/482 (78%), Gaps = 1/482 (0%)
 Frame = +3

Query: 132  LILKSFLSHHRRNPNHLPTVPAVPGVPLLGNLLQLKEKKPYKTFTKWAETYGPIYSIKAG 311
            + ++ F+S+ +R+   LP VP +PG+PL+GNLLQLKEKKP+KTF +WAETYGPI+SI+ G
Sbjct: 26   IFIRRFVSNRKRSVEGLPPVPDIPGLPLIGNLLQLKEKKPHKTFARWAETYGPIFSIRTG 85

Query: 312  ATSMVVVSSNHLAKEAMVTRFDSISTRKLSKALRILTADKTMVAMSDYDDYHKTVKRNIL 491
            A++M+V++S+ +AKEAMVTRF SISTRKLS AL+ILT DK MVA SDY+D+HK VK  IL
Sbjct: 86   ASTMIVLNSSEVAKEAMVTRFSSISTRKLSNALKILTFDKCMVATSDYNDFHKMVKGFIL 145

Query: 492  TSVLGPAARKKHRAHRDTMADNVSNQLHALALTSHREAINLREILQSELFTLAFKQVMGK 671
             +VLG  A+K+HR HRDT+ +N+S  LHA   TS  E + L++I +SE+F LA KQ +GK
Sbjct: 146  RNVLGAPAQKRHRCHRDTLIENISKYLHAHVKTSPLEPVVLKKIFESEIFGLALKQALGK 205

Query: 672  DFESIYVGDLGITMTKEEVFQVLVIDPMMGAIDVDWRDFFPYLRWIPNTSFEKKIEQMDI 851
            D ESIYV +LG T+++EE+F VLV+DPM GAI+VDWRDFFPYL WIPN S E KI++MD 
Sbjct: 206  DIESIYVEELGTTLSREEIFAVLVVDPMAGAIEVDWRDFFPYLSWIPNKSMEMKIQRMDF 265

Query: 852  RREAVMKTLIQEHRKRIDSGETLNSYIDYLLSEAQPLTEKQLLMSLWEPIIETSDTTMVT 1031
            RR A+MK LI E +KRI SGE  NSYID+LLSEA  LTEKQ+ M +WE IIE SDTT+VT
Sbjct: 266  RRGALMKALIGEQKKRIGSGEEKNSYIDFLLSEATTLTEKQIAMLIWETIIEISDTTLVT 325

Query: 1032 TEWAMYELAKNPKKQDRLYDEIRNVCGSQKITEEKLCQMPYLSAIFHETLRRHSPVPIIP 1211
            +EWAMYELAK+P +Q+ LY EI  VCGS K+TEE L ++PYL+++FHETLR++SP P++P
Sbjct: 326  SEWAMYELAKDPNRQEILYREIHKVCGSNKLTEENLSKLPYLNSVFHETLRKYSPAPMVP 385

Query: 1212 LRYVHENTVLGGYHVPAGTELAINIYGCNMXXXXXXXXXXXXXXRFLAEN-EPIDLQRTM 1388
            +RY HE+T LGGYH+PAG+++AINIYGCNM              RFL E  + +DL +TM
Sbjct: 386  VRYAHEDTQLGGYHIPAGSQIAINIYGCNMNKKQWENPEEWKPERFLDEKYDLMDLHKTM 445

Query: 1389 AFGGGKRVCAGAMQAMLIACVCMGRMVQEFEWRLKDDTGEDVSTIGLTTQMLNPMLAVIK 1568
            AFGGGKRVCAGA+QAMLIAC  +GR VQEFEW+L     E+V T+ LT+Q L+PM A+IK
Sbjct: 446  AFGGGKRVCAGALQAMLIACTSIGRFVQEFEWKLMGGEEENVDTVALTSQKLHPMQAIIK 505

Query: 1569 PR 1574
             R
Sbjct: 506  AR 507