BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00015218
         (1527 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFO64617.1| cytochrome P450 mono-oxygenase [Artemisia annua]       399   e-143
emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]                406   e-142
emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]                406   e-142
ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   372   e-124
ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis ...   372   e-122

>gb|AFO64617.1| cytochrome P450 mono-oxygenase [Artemisia annua]
          Length = 502

 Score =  399 bits (1026), Expect(3) = e-143
 Identities = 194/276 (70%), Positives = 224/276 (81%)
 Frame = +1

Query: 493  LSWLGVKGSEKKLIALQKKRTSFFQGLIDQLRXXXXXXXXXXXXXXXTMIEVLLQLQETD 672
            L W GV   EKKL+AL++KR  FFQGLI+QLR               TMIEVLL LQE+D
Sbjct: 226  LRWFGVTKLEKKLVALREKREVFFQGLIEQLRKPKGAEVGNNKK---TMIEVLLSLQESD 282

Query: 673  PEYYTDELIKTFVLNILAAGIDTSTATMEWAFSLLLNHPDVLKKAQKEIDSHVGENRLVD 852
            P+YYTDE+I++FVL +L+AG DTS  TMEWA SLLLNHP VLKKAQ EID  VG +RLVD
Sbjct: 283  PKYYTDEMIRSFVLVLLSAGTDTSAGTMEWAMSLLLNHPQVLKKAQNEIDRVVGNDRLVD 342

Query: 853  ESDMASLPYLRCILNETLRMYPAGPLLVPHESSEDCVVGGYHIPRGTMLLVNQWAIHRDP 1032
            ESD+ +LPYLRCI+NETLR+ P GPLLVPHESSEDCV+GGY+IPRGTMLLVNQWAIH DP
Sbjct: 343  ESDVVNLPYLRCIINETLRICPPGPLLVPHESSEDCVIGGYNIPRGTMLLVNQWAIHHDP 402

Query: 1033 DLWSDPESFIPERFQGVEGPRDGFRFMPFGYGRRSCPGEGLAMRMVGLTLGLLIQCFDWE 1212
             LW+DPE F PERF+G+EG RDGF+ MPFG GRRSCPGEGLA+R++G TLGLLIQCFDWE
Sbjct: 403  KLWTDPEMFKPERFEGLEGTRDGFKLMPFGSGRRSCPGEGLAVRVIGSTLGLLIQCFDWE 462

Query: 1213 RISEDMVDMTEGAGLTMPKAQPCVAKCTPRPMMQNV 1320
            R+SE MVDM+E  GLTMPKA+P VAKC PR  +Q +
Sbjct: 463  RLSEKMVDMSEAPGLTMPKAEPLVAKCKPRLEIQTL 498



 Score =  102 bits (254), Expect(3) = e-143
 Identities = 48/65 (73%), Positives = 57/65 (87%)
 Frame = +2

Query: 2   DHWRNLRRISTVEIFSSHRLNEFHDVRADEGRLLIRKLISECSSPVNLKSVLNELTLNVM 181
           D+WRNLRRI+++EI S HRLNEFHD+R DEG+LL+RKL+S  SS VN+KSV  ELTLNVM
Sbjct: 120 DNWRNLRRIASIEILSIHRLNEFHDIRVDEGKLLVRKLVSNSSSSVNVKSVFYELTLNVM 179

Query: 182 MRMIS 196
           MRMIS
Sbjct: 180 MRMIS 184



 Score = 57.8 bits (138), Expect(3) = e-143
 Identities = 27/46 (58%), Positives = 36/46 (78%), Gaps = 3/46 (6%)
 Frame = +3

Query: 279 ISGKRYFSGEMVE---EGKRFQEIVEETFQVGGASNLVDHLPILSW 407
           ISGKRYF G++ E   EGKRF+EI++ETF + GA+N+ D+LP L W
Sbjct: 183 ISGKRYFGGDIPEVEAEGKRFREILDETFLLAGAANVGDYLPFLRW 228


>emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]
          Length = 520

 Score =  406 bits (1044), Expect(3) = e-142
 Identities = 195/276 (70%), Positives = 227/276 (82%)
 Frame = +1

Query: 493  LSWLGVKGSEKKLIALQKKRTSFFQGLIDQLRXXXXXXXXXXXXXXXTMIEVLLQLQETD 672
            LSWLGVKG EKKLI LQ+KR  FFQGLIDQLR               TMIE+LL LQET+
Sbjct: 244  LSWLGVKGLEKKLIKLQEKRDVFFQGLIDQLRKSKGTEDVNKKK---TMIELLLSLQETE 300

Query: 673  PEYYTDELIKTFVLNILAAGIDTSTATMEWAFSLLLNHPDVLKKAQKEIDSHVGENRLVD 852
            PEYYTD +I++FVL +LAAG DTS  TMEW  SLLLNHP VLKKAQ EIDS +G+N LVD
Sbjct: 301  PEYYTDAMIRSFVLVLLAAGSDTSAGTMEWVMSLLLNHPQVLKKAQNEIDSVIGKNCLVD 360

Query: 853  ESDMASLPYLRCILNETLRMYPAGPLLVPHESSEDCVVGGYHIPRGTMLLVNQWAIHRDP 1032
            ESD+ +LPYLRCI+NETLR+YPAGPLLVPHE+S DCVVGGY++PRGT+L+VNQWAIH DP
Sbjct: 361  ESDIPNLPYLRCIINETLRLYPAGPLLVPHEASSDCVVGGYNVPRGTILIVNQWAIHHDP 420

Query: 1033 DLWSDPESFIPERFQGVEGPRDGFRFMPFGYGRRSCPGEGLAMRMVGLTLGLLIQCFDWE 1212
             +W +PE+F PERF+G+EG RDGF+ +PFG GRRSCPGEGLA+RM+G+TLG +IQCFDWE
Sbjct: 421  KVWDEPETFKPERFEGLEGTRDGFKLLPFGSGRRSCPGEGLAVRMLGMTLGSIIQCFDWE 480

Query: 1213 RISEDMVDMTEGAGLTMPKAQPCVAKCTPRPMMQNV 1320
            R SE++VDMTEG GLTMPKA P VAKC PR  M N+
Sbjct: 481  RTSEELVDMTEGPGLTMPKAIPLVAKCKPRVEMTNL 516



 Score = 89.0 bits (219), Expect(3) = e-142
 Identities = 45/68 (66%), Positives = 54/68 (79%), Gaps = 3/68 (4%)
 Frame = +2

Query: 2   DHWRNLRRISTVEIFSSHRLNEFHDVRADEGRLLIRKLISEC---SSPVNLKSVLNELTL 172
           D+WRNLRRI+++EI S HRLNEFHD+R +E RLLI+KL+S C   SS V +K    ELTL
Sbjct: 135 DNWRNLRRIASIEILSIHRLNEFHDIRVEETRLLIQKLLSACNSGSSQVTMKFSFYELTL 194

Query: 173 NVMMRMIS 196
           NVMMRMIS
Sbjct: 195 NVMMRMIS 202



 Score = 61.2 bits (147), Expect(3) = e-142
 Identities = 28/46 (60%), Positives = 38/46 (82%), Gaps = 3/46 (6%)
 Frame = +3

Query: 279 ISGKRYFSG---EMVEEGKRFQEIVEETFQVGGASNLVDHLPILSW 407
           ISGKRYF G   E+ EEGKRF+++++ETF + GASN+ D+LP+LSW
Sbjct: 201 ISGKRYFGGDNPELEEEGKRFRDMLDETFVLAGASNVGDYLPVLSW 246


>emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]
          Length = 505

 Score =  406 bits (1044), Expect(3) = e-142
 Identities = 195/276 (70%), Positives = 227/276 (82%)
 Frame = +1

Query: 493  LSWLGVKGSEKKLIALQKKRTSFFQGLIDQLRXXXXXXXXXXXXXXXTMIEVLLQLQETD 672
            LSWLGVKG EKKLI LQ+KR  FFQGLIDQLR               TMIE+LL LQET+
Sbjct: 229  LSWLGVKGLEKKLIKLQEKRDVFFQGLIDQLRKSKGTEDVNKKK---TMIELLLSLQETE 285

Query: 673  PEYYTDELIKTFVLNILAAGIDTSTATMEWAFSLLLNHPDVLKKAQKEIDSHVGENRLVD 852
            PEYYTD +I++FVL +LAAG DTS  TMEW  SLLLNHP VLKKAQ EIDS +G+N LVD
Sbjct: 286  PEYYTDAMIRSFVLVLLAAGSDTSAGTMEWVMSLLLNHPQVLKKAQNEIDSVIGKNCLVD 345

Query: 853  ESDMASLPYLRCILNETLRMYPAGPLLVPHESSEDCVVGGYHIPRGTMLLVNQWAIHRDP 1032
            ESD+ +LPYLRCI+NETLR+YPAGPLLVPHE+S DCVVGGY++PRGT+L+VNQWAIH DP
Sbjct: 346  ESDIPNLPYLRCIINETLRLYPAGPLLVPHEASSDCVVGGYNVPRGTILIVNQWAIHHDP 405

Query: 1033 DLWSDPESFIPERFQGVEGPRDGFRFMPFGYGRRSCPGEGLAMRMVGLTLGLLIQCFDWE 1212
             +W +PE+F PERF+G+EG RDGF+ +PFG GRRSCPGEGLA+RM+G+TLG +IQCFDWE
Sbjct: 406  KVWDEPETFKPERFEGLEGTRDGFKLLPFGSGRRSCPGEGLAVRMLGMTLGSIIQCFDWE 465

Query: 1213 RISEDMVDMTEGAGLTMPKAQPCVAKCTPRPMMQNV 1320
            R SE++VDMTEG GLTMPKA P VAKC PR  M N+
Sbjct: 466  RTSEELVDMTEGPGLTMPKAIPLVAKCKPRVEMTNL 501



 Score = 89.0 bits (219), Expect(3) = e-142
 Identities = 45/68 (66%), Positives = 54/68 (79%), Gaps = 3/68 (4%)
 Frame = +2

Query: 2   DHWRNLRRISTVEIFSSHRLNEFHDVRADEGRLLIRKLISEC---SSPVNLKSVLNELTL 172
           D+WRNLRRI+++EI S HRLNEFHD+R +E RLLI+KL+S C   SS V +K    ELTL
Sbjct: 120 DNWRNLRRIASIEILSIHRLNEFHDIRVEETRLLIQKLLSACNSGSSQVTMKFSFYELTL 179

Query: 173 NVMMRMIS 196
           NVMMRMIS
Sbjct: 180 NVMMRMIS 187



 Score = 61.2 bits (147), Expect(3) = e-142
 Identities = 28/46 (60%), Positives = 38/46 (82%), Gaps = 3/46 (6%)
 Frame = +3

Query: 279 ISGKRYFSG---EMVEEGKRFQEIVEETFQVGGASNLVDHLPILSW 407
           ISGKRYF G   E+ EEGKRF+++++ETF + GASN+ D+LP+LSW
Sbjct: 186 ISGKRYFGGDNPELEEEGKRFRDMLDETFVLAGASNVGDYLPVLSW 231


>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 499

 Score =  372 bits (954), Expect(3) = e-124
 Identities = 178/276 (64%), Positives = 213/276 (77%)
 Frame = +1

Query: 493  LSWLGVKGSEKKLIALQKKRTSFFQGLIDQLRXXXXXXXXXXXXXXXTMIEVLLQLQETD 672
            L WLG+KG+E++LI LQK+R SF Q LI++ R               TMIEVLL LQET+
Sbjct: 224  LGWLGLKGTERRLIKLQKRRESFIQNLIEEHRRKGSNCEGRQK----TMIEVLLSLQETE 279

Query: 673  PEYYTDELIKTFVLNILAAGIDTSTATMEWAFSLLLNHPDVLKKAQKEIDSHVGENRLVD 852
            PEYYTDE+I+  +L++L  G DTS  TMEWA SLLLN+P VLKKA +EID  +G +RL++
Sbjct: 280  PEYYTDEIIRGLMLSMLTGGTDTSAGTMEWALSLLLNNPKVLKKAHQEIDDRLGHDRLIE 339

Query: 853  ESDMASLPYLRCILNETLRMYPAGPLLVPHESSEDCVVGGYHIPRGTMLLVNQWAIHRDP 1032
            E D+A LPYLR I+ ETLRMYPAGPLLVPHESS++C VGG+ IP+GTMLLVN WAI  D 
Sbjct: 340  ELDLAQLPYLRSIIKETLRMYPAGPLLVPHESSKECSVGGFRIPQGTMLLVNLWAIQSDH 399

Query: 1033 DLWSDPESFIPERFQGVEGPRDGFRFMPFGYGRRSCPGEGLAMRMVGLTLGLLIQCFDWE 1212
             +W DP  F PERF+GVEG RDGF+F+PFG GRR CPGE LA+R+VGL LG LIQCFDWE
Sbjct: 400  KIWGDPTEFRPERFEGVEGDRDGFKFVPFGSGRRGCPGEALAIRIVGLALGSLIQCFDWE 459

Query: 1213 RISEDMVDMTEGAGLTMPKAQPCVAKCTPRPMMQNV 1320
            R+ E MVDMTEG GLT+PKAQP +AKC PRP M N+
Sbjct: 460  RVDEQMVDMTEGGGLTLPKAQPLLAKCRPRPTMVNL 495



 Score = 71.2 bits (173), Expect(3) = e-124
 Identities = 36/64 (56%), Positives = 44/64 (68%)
 Frame = +2

Query: 2   DHWRNLRRISTVEIFSSHRLNEFHDVRADEGRLLIRKLISECSSPVNLKSVLNELTLNVM 181
           DHWRNLRRIS+ EI SS+RL     +R+DE R L+R L    S  V +KS   E+TLNVM
Sbjct: 119 DHWRNLRRISSFEILSSNRLQTLSGIRSDEVRSLVRWLFKNQSQMVEMKSAFFEMTLNVM 178

Query: 182 MRMI 193
           M+MI
Sbjct: 179 MKMI 182



 Score = 53.9 bits (128), Expect(3) = e-124
 Identities = 25/45 (55%), Positives = 33/45 (73%), Gaps = 2/45 (4%)
 Frame = +3

Query: 279 ISGKRYFSGEM--VEEGKRFQEIVEETFQVGGASNLVDHLPILSW 407
           I GKRY+   +  VEE + F+E+V ETFQ+ GA+N+VD LPIL W
Sbjct: 182 IGGKRYYGENIGEVEEARMFREMVSETFQLAGATNMVDFLPILGW 226


>ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
          Length = 498

 Score =  372 bits (956), Expect(3) = e-122
 Identities = 178/276 (64%), Positives = 214/276 (77%)
 Frame = +1

Query: 493  LSWLGVKGSEKKLIALQKKRTSFFQGLIDQLRXXXXXXXXXXXXXXXTMIEVLLQLQETD 672
            L WLG+KG+EK+L+ LQK R SF Q LI++ R               TMIEVLL LQET+
Sbjct: 223  LGWLGLKGTEKRLMELQKMRDSFIQNLIEEHRIKGSKCERRPK----TMIEVLLSLQETE 278

Query: 673  PEYYTDELIKTFVLNILAAGIDTSTATMEWAFSLLLNHPDVLKKAQKEIDSHVGENRLVD 852
            PE YTDE+I   +L++L AG DTS  TMEWA SLLLN P+VLKKAQ+EID H+G +RL++
Sbjct: 279  PENYTDEIIGGLMLSMLTAGTDTSAGTMEWALSLLLNSPEVLKKAQQEIDVHLGHDRLIE 338

Query: 853  ESDMASLPYLRCILNETLRMYPAGPLLVPHESSEDCVVGGYHIPRGTMLLVNQWAIHRDP 1032
            E D+A LPYLR I+ ETLRMYPAGPLL+PHESS++C VGG+ IP GTMLLVN WAIH DP
Sbjct: 339  EVDLAQLPYLRSIIKETLRMYPAGPLLIPHESSKECFVGGFRIPPGTMLLVNVWAIHNDP 398

Query: 1033 DLWSDPESFIPERFQGVEGPRDGFRFMPFGYGRRSCPGEGLAMRMVGLTLGLLIQCFDWE 1212
             +W++P  F PERF+G EG RDG RF+PFG GRR CPGEGLA+RMVGL +G LIQCFDWE
Sbjct: 399  KIWAEPTKFKPERFEGEEGERDGLRFLPFGSGRRGCPGEGLAIRMVGLAMGSLIQCFDWE 458

Query: 1213 RISEDMVDMTEGAGLTMPKAQPCVAKCTPRPMMQNV 1320
            R+ + MVDMTEG GL++PKAQP +AKC PRP M N+
Sbjct: 459  RVDQQMVDMTEGHGLSIPKAQPLLAKCRPRPTMVNL 494



 Score = 70.9 bits (172), Expect(3) = e-122
 Identities = 36/64 (56%), Positives = 45/64 (70%)
 Frame = +2

Query: 2   DHWRNLRRISTVEIFSSHRLNEFHDVRADEGRLLIRKLISECSSPVNLKSVLNELTLNVM 181
           DHWRNLRRIS+ EI SS+RL     +R+DE R LI +L+   +  V +KS   ELTLNV+
Sbjct: 119 DHWRNLRRISSFEILSSNRLQMLSGIRSDEVRTLICRLVKNQNQVVEMKSAFFELTLNVI 178

Query: 182 MRMI 193
           MRMI
Sbjct: 179 MRMI 182



 Score = 43.9 bits (102), Expect(3) = e-122
 Identities = 21/45 (46%), Positives = 32/45 (71%), Gaps = 2/45 (4%)
 Frame = +3

Query: 279 ISGKRYFSGEM--VEEGKRFQEIVEETFQVGGASNLVDHLPILSW 407
           I GKRY+   +  VEE ++F+E+V + F++ G +N+VD LPIL W
Sbjct: 182 IGGKRYYGENVGEVEEARKFREMVSKAFRLAG-TNMVDFLPILGW 225