BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00014233
         (1359 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAE16979.1| steroid 22-alpha-hydroxylase [Zinnia elegans]         721   0.0  
ref|XP_002267187.1| PREDICTED: cytochrome P450 90B1-like [Vitis ...   680   0.0  
emb|CBI17531.3| unnamed protein product [Vitis vinifera]              678   0.0  
ref|XP_002310224.1| cytochrome P450 probable campestanol to 6-de...   672   0.0  
ref|XP_002327809.1| predicted protein [Populus trichocarpa] gi|2...   670   0.0  

>dbj|BAE16979.1| steroid 22-alpha-hydroxylase [Zinnia elegans]
          Length = 496

 Score =  721 bits (1861), Expect = 0.0
 Identities = 357/437 (81%), Positives = 384/437 (87%)
 Frame = -3

Query: 1312 TNKMSDYEFFIFXXXXXXXXXXXXXLAKRRTQNTPTLNLPPGNMGWPFIGETIGYLRPYS 1133
            T  M D EFFI              L KRRT N  T NLPPGNMGWPFIGETIGYL+PYS
Sbjct: 5    TLNMCDLEFFILASCLVLALFLILKLVKRRTNNGSTRNLPPGNMGWPFIGETIGYLQPYS 64

Query: 1132 ATTIGTFMEQHISRFGKIFKSNLFGEPTIVSVDPGLNRYILQNEGRLFECSYPRSIGGIL 953
            ATTIG FMEQHIS++GKI+KS+LFGEPTIVS DPGLN+YILQNEGRLFECSYPRSIGGIL
Sbjct: 65   ATTIGKFMEQHISKYGKIYKSSLFGEPTIVSADPGLNKYILQNEGRLFECSYPRSIGGIL 124

Query: 952  GKWSMLVLVGDMHRDMRLISLNFLTNARLKTHLLREVEKNTLWVLDSWQENSPFSAQDEA 773
            GKWSMLVLVGDMHRDMR ISLNFL+NARLKT L+ EVEKNTLWVLDSW+ENSPF AQ+EA
Sbjct: 125  GKWSMLVLVGDMHRDMRQISLNFLSNARLKTQLVNEVEKNTLWVLDSWKENSPFCAQEEA 184

Query: 772  KKFTFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALKSRSTILK 593
            KKFTFNLMA HIMSLDPG+PETE+LKKEYVTFMKGVVS PLNFPGTAY KALKSR+TILK
Sbjct: 185  KKFTFNLMATHIMSLDPGEPETERLKKEYVTFMKGVVSPPLNFPGTAYWKALKSRATILK 244

Query: 592  FIEMRMEERIRKFGEGNDVEKLEDDLLGWVLKNSNLSKEQILDLVLSLLFAGHETSSVSI 413
            FIE +MEERIR   EGN + KL++DLLGW +KNSNL+KEQILDLVLSLLFAGHETSSVSI
Sbjct: 245  FIETKMEERIR-MDEGNGLGKLDNDLLGWSMKNSNLTKEQILDLVLSLLFAGHETSSVSI 303

Query: 412  ALAIYFLEACPTAVHQLREEHEEIANAKKQSGVKDLSWDDYKKMEFTQCVINETLRFGNV 233
            +LA+YFLEACPTAV QLREEHEEI   KK  G K L+WDDYKKMEFTQCVINETLRFGNV
Sbjct: 304  SLAVYFLEACPTAVRQLREEHEEIVMKKKLLGEKYLTWDDYKKMEFTQCVINETLRFGNV 363

Query: 232  VRFLHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDPTIFDQPHLFNPWRWQNARVTSSSG 53
            VRFLHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDPT FDQP+LF+PWRWQNA VTSS+ 
Sbjct: 364  VRFLHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDPTHFDQPYLFDPWRWQNASVTSSTC 423

Query: 52   ATTTSPNNFMPFGGGPR 2
            +T  S +NFMPFGGGPR
Sbjct: 424  STPPSASNFMPFGGGPR 440


>ref|XP_002267187.1| PREDICTED: cytochrome P450 90B1-like [Vitis vinifera]
          Length = 487

 Score =  680 bits (1754), Expect = 0.0
 Identities = 342/436 (78%), Positives = 373/436 (85%), Gaps = 2/436 (0%)
 Frame = -3

Query: 1303 MSDYEFFIFXXXXXXXXXXXXXLAKRRTQNTPTLNLPPGNMGWPFIGETIGYLRPYSATT 1124
            MSD EFF+              L KR+      LNLPPGN GWPF+GETIGYL+PYSAT+
Sbjct: 1    MSDLEFFLLLLASISVPLVILNLIKRKQSG---LNLPPGNTGWPFLGETIGYLKPYSATS 57

Query: 1123 IGTFMEQHISRFGKIFKSNLFGEPTIVSVDPGLNRYILQNEGRLFECSYPRSIGGILGKW 944
            IG FMEQHISRFG+I+KSNLFGEPTIVS D GLNR+ILQNEG+LFECSYPRSIGGILGKW
Sbjct: 58   IGDFMEQHISRFGEIYKSNLFGEPTIVSADSGLNRFILQNEGKLFECSYPRSIGGILGKW 117

Query: 943  SMLVLVGDMHRDMRLISLNFLTNARLKTHLLREVEKNTLWVLDSWQENSPFSAQDEAKKF 764
            SMLVLVGDMHRDMR ISLNFL++ RL+THLL EV K+TL VL SW+EN  FSAQDEAKKF
Sbjct: 118  SMLVLVGDMHRDMRTISLNFLSHGRLRTHLLPEVVKHTLLVLSSWKENCTFSAQDEAKKF 177

Query: 763  TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALKSRSTILKFIE 584
            TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKAL+SRSTILKFIE
Sbjct: 178  TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALQSRSTILKFIE 237

Query: 583  MRMEERIRKFGEGNDVEKLEDDLLGWVLKNSNLSKEQILDLVLSLLFAGHETSSVSIALA 404
            ++MEERI+K   G      +DDLLGWVLK+SNLS EQILDLVLSLLFAGHETSSV+IALA
Sbjct: 238  LKMEERIQKLRGGGFENMEDDDLLGWVLKHSNLSTEQILDLVLSLLFAGHETSSVAIALA 297

Query: 403  IYFLEACPTAVHQLREEHEEIANAKKQSGVKDLSWDDYKKMEFTQCVINETLRFGNVVRF 224
            IYFLE CP AV QLREEH EI+ AKKQSG  +LSW+DYKKMEFTQCVI+ETLR GNVVRF
Sbjct: 298  IYFLEGCPNAVQQLREEHLEISRAKKQSGELELSWEDYKKMEFTQCVISETLRLGNVVRF 357

Query: 223  LHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDPTIFDQPHLFNPWRWQN--ARVTSSSGA 50
            LHRKA+KDVRYKGYDIPCGWKVLPVIAAVHLDP++FDQP  FNPWRWQN   R  S+S +
Sbjct: 358  LHRKALKDVRYKGYDIPCGWKVLPVIAAVHLDPSLFDQPQHFNPWRWQNNGNRGNSTSWS 417

Query: 49   TTTSPNNFMPFGGGPR 2
            T T+  NFMPFGGGPR
Sbjct: 418  TATN-QNFMPFGGGPR 432


>emb|CBI17531.3| unnamed protein product [Vitis vinifera]
          Length = 488

 Score =  678 bits (1750), Expect = 0.0
 Identities = 339/436 (77%), Positives = 371/436 (85%), Gaps = 2/436 (0%)
 Frame = -3

Query: 1303 MSDYEFFIFXXXXXXXXXXXXXLAKRRTQNTPTLNLPPGNMGWPFIGETIGYLRPYSATT 1124
            MSD EFF+              L KR+      LNLPPGN GWPF+GETIGYL+PYSAT+
Sbjct: 1    MSDLEFFLLLLASISVPLVILNLIKRKQSG---LNLPPGNTGWPFLGETIGYLKPYSATS 57

Query: 1123 IGTFMEQHISRFGKIFKSNLFGEPTIVSVDPGLNRYILQNEGRLFECSYPRSIGGILGKW 944
            IG FMEQHISRFG+I+KSNLFGEPTIVS D GLNR+ILQNEG+LFECSYPRSIGGILGKW
Sbjct: 58   IGDFMEQHISRFGEIYKSNLFGEPTIVSADSGLNRFILQNEGKLFECSYPRSIGGILGKW 117

Query: 943  SMLVLVGDMHRDMRLISLNFLTNARLKTHLLREVEKNTLWVLDSWQENSPFSAQDEAKKF 764
            SMLVLVGDMHRDMR ISLNFL++ RL+THLL EV K+TL VL SW+EN  FSAQDEAKKF
Sbjct: 118  SMLVLVGDMHRDMRTISLNFLSHGRLRTHLLPEVVKHTLLVLSSWKENCTFSAQDEAKKF 177

Query: 763  TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALKSRSTILKFIE 584
            TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKAL+SRSTILKFIE
Sbjct: 178  TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALQSRSTILKFIE 237

Query: 583  MRMEERIRKFGEGNDVEKLEDDLLGWVLKNSNLSKEQILDLVLSLLFAGHETSSVSIALA 404
            ++MEERI+K   G      +DDLLGWVLK+SNLS EQILDLVLSLLFAGHETSSV+IALA
Sbjct: 238  LKMEERIQKLRGGGFENMEDDDLLGWVLKHSNLSTEQILDLVLSLLFAGHETSSVAIALA 297

Query: 403  IYFLEACPTAVHQLREEHEEIANAKKQSGVKDLSWDDYKKMEFTQCVINETLRFGNVVRF 224
            IYFLE CP AV QLREEH EI+ AKKQSG  +LSW+DYKKMEFTQCVI+ETLR GNVVRF
Sbjct: 298  IYFLEGCPNAVQQLREEHLEISRAKKQSGELELSWEDYKKMEFTQCVISETLRLGNVVRF 357

Query: 223  LHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDPTIFDQPHLFNPWRWQ--NARVTSSSGA 50
            LHRKA+KDVRYKGYDIPCGWKVLPVIAAVHLDP++FDQP  FNPWRWQ  N    +S+  
Sbjct: 358  LHRKALKDVRYKGYDIPCGWKVLPVIAAVHLDPSLFDQPQHFNPWRWQQNNGNRGNSTSW 417

Query: 49   TTTSPNNFMPFGGGPR 2
            +T +  NFMPFGGGPR
Sbjct: 418  STATNQNFMPFGGGPR 433


>ref|XP_002310224.1| cytochrome P450 probable campestanol to 6-deoxocathasterone or
            6-oxocampestanol to cathasterone [Populus trichocarpa]
            gi|222853127|gb|EEE90674.1| cytochrome P450 probable
            campestanol to 6-deoxocathasterone or 6-oxocampestanol to
            cathasterone [Populus trichocarpa]
          Length = 486

 Score =  672 bits (1735), Expect = 0.0
 Identities = 332/404 (82%), Positives = 364/404 (90%), Gaps = 4/404 (0%)
 Frame = -3

Query: 1201 NLPPGNMGWPFIGETIGYLRPYSATTIGTFMEQHISRFGKIFKSNLFGEPTIVSVDPGLN 1022
            NLPPGNMGWPF+GETIGYL+PYSAT+IG FMEQHISR+GKI+KSNLFGEPTIVS D GLN
Sbjct: 32   NLPPGNMGWPFLGETIGYLKPYSATSIGEFMEQHISRYGKIYKSNLFGEPTIVSADAGLN 91

Query: 1021 RYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRLISLNFLTNARLKTHLLREV 842
            R+ILQNEGRLFECSYP+SIGGILGKWSMLVLVGDMHRDMR+ISLNFL++ARL+THLL+EV
Sbjct: 92   RFILQNEGRLFECSYPKSIGGILGKWSMLVLVGDMHRDMRIISLNFLSHARLRTHLLKEV 151

Query: 841  EKNTLWVLDSWQENSPFSAQDEAKKFTFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVV 662
            EK TL VL SW+EN  FSAQDEAKKFTFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVV
Sbjct: 152  EKQTLLVLSSWKENCTFSAQDEAKKFTFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVV 211

Query: 661  SAPLNFPGTAYRKALKSRSTILKFIEMRMEERIRKFGEGNDVEKLE-DDLLGWVLKNSNL 485
            SAP+N PGT YRKALKSRS ILKFIE +MEERI +   G  VE LE DDLLGWVLK+SNL
Sbjct: 212  SAPINLPGTPYRKALKSRSIILKFIERKMEERIAETKGG--VENLEDDDLLGWVLKHSNL 269

Query: 484  SKEQILDLVLSLLFAGHETSSVSIALAIYFLEACPTAVHQLREEHEEIANAKKQSGVKDL 305
            S EQILDL+LSLLFAGHETSSVSIALAIYFL+ACP A+ QL+EEH +I+ AKK+SG  +L
Sbjct: 270  STEQILDLILSLLFAGHETSSVSIALAIYFLQACPGAIQQLKEEHIQISRAKKRSGETEL 329

Query: 304  SWDDYKKMEFTQCVINETLRFGNVVRFLHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDP 125
            +WDDYKKMEFTQCVI+ETLR GNVVRFLHRKA+KDVRYKGYDIPCGWKVLPVI+AVHLD 
Sbjct: 330  NWDDYKKMEFTQCVISETLRLGNVVRFLHRKALKDVRYKGYDIPCGWKVLPVISAVHLDS 389

Query: 124  TIFDQPHLFNPWRWQ---NARVTSSSGATTTSPNNFMPFGGGPR 2
            T+FDQP  FNPWRWQ   NAR  S+S + TTS N FMPFGGGPR
Sbjct: 390  TLFDQPQHFNPWRWQQHNNARGASTSSSGTTSSNYFMPFGGGPR 433


>ref|XP_002327809.1| predicted protein [Populus trichocarpa] gi|222836894|gb|EEE75287.1|
            predicted protein [Populus trichocarpa]
          Length = 488

 Score =  670 bits (1728), Expect = 0.0
 Identities = 330/404 (81%), Positives = 365/404 (90%), Gaps = 4/404 (0%)
 Frame = -3

Query: 1201 NLPPGNMGWPFIGETIGYLRPYSATTIGTFMEQHISRFGKIFKSNLFGEPTIVSVDPGLN 1022
            NLPPGNMGWPF+GETIGYL+PYSAT+IG FMEQHISR+GKI+KSNLFGEPTIVS D GLN
Sbjct: 32   NLPPGNMGWPFLGETIGYLKPYSATSIGEFMEQHISRYGKIYKSNLFGEPTIVSADAGLN 91

Query: 1021 RYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRLISLNFLTNARLKTHLLREV 842
            R+ILQ+EGRLFECSYPRSIGGILGKWSMLVLVGDMHR+MR ISLNFL++ARL++HLL+EV
Sbjct: 92   RFILQSEGRLFECSYPRSIGGILGKWSMLVLVGDMHRNMRSISLNFLSHARLRSHLLKEV 151

Query: 841  EKNTLWVLDSWQENSPFSAQDEAKKFTFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVV 662
            EK TL VL SW+EN  FSAQDEAKKFTFNLMAKH+MSLDPGKPETEQLKKEYVTFMKGVV
Sbjct: 152  EKQTLLVLSSWKENCTFSAQDEAKKFTFNLMAKHMMSLDPGKPETEQLKKEYVTFMKGVV 211

Query: 661  SAPLNFPGTAYRKALKSRSTILKFIEMRMEERIRKFGEG-NDVEKLEDDLLGWVLKNSNL 485
            SAPLNFPGT YRKALKSRS ILKFIE +MEERI +  EG  D+E  EDDLLGWVLK+SNL
Sbjct: 212  SAPLNFPGTPYRKALKSRSIILKFIEQKMEERIARLKEGVEDLE--EDDLLGWVLKHSNL 269

Query: 484  SKEQILDLVLSLLFAGHETSSVSIALAIYFLEACPTAVHQLREEHEEIANAKKQSGVKDL 305
            S EQILDL+LSLLFAGHETSSVSIALAIYFL+ACP A+ QL+EEH EI+ AKKQSG  ++
Sbjct: 270  STEQILDLILSLLFAGHETSSVSIALAIYFLQACPGAIQQLKEEHIEISRAKKQSGEMEI 329

Query: 304  SWDDYKKMEFTQCVINETLRFGNVVRFLHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDP 125
            +WDDYKKMEFTQCVI+ETLR GNVVRFLHRKA++DVRYKGYDIPCGWKVLPVI+AVHLD 
Sbjct: 330  NWDDYKKMEFTQCVISETLRLGNVVRFLHRKAVRDVRYKGYDIPCGWKVLPVISAVHLDS 389

Query: 124  TIFDQPHLFNPWRWQ--NARVTSS-SGATTTSPNNFMPFGGGPR 2
            T+FDQP  FNPWRWQ  NAR +S+ S A   S N+FMPFGGGPR
Sbjct: 390  TVFDQPQQFNPWRWQHNNARGSSTCSSAAAASSNHFMPFGGGPR 433