BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00014231
         (3030 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   959   0.0  
gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]               943   0.0  
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   935   0.0  
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ...   897   0.0  
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   896   0.0  

>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  959 bits (2478), Expect = 0.0
 Identities = 494/884 (55%), Positives = 621/884 (70%), Gaps = 40/884 (4%)
 Frame = -3

Query: 2779 MAMLSLSSATQPPYLTCRTTNLSKLIVADSCKYGRRVARSLGRMDMERSLVFGEVAKNMI 2600
            M  L  S   +P  + C   + SKL+ A     G +VA  L +MD+ER  + G VA+N  
Sbjct: 1    MTTLIPSLVIRPCCVNCGARDSSKLVAATHYICGNKVAHGLEKMDLERKEILGVVAQNAT 60

Query: 2599 KGSCHDLGVGVLAASTLPVIQTVDKLST-----------YLFRTEKGGHVKVITRLKDDK 2453
            +    ++   V A S + + +T ++ ST           YLF TE GG VKVI   K+ K
Sbjct: 61   RNCFRNINWKVSATSHIAIEETENRYSTTESEELESRLNYLFLTEIGGQVKVIVGKKNKK 120

Query: 2452 YGVDVEVSSLQLSGNDDDVIMSWGIFRXXXXXXXXXXXXXS------GIFETPFVNKSLN 2291
            Y V +EVSSLQL  +D+ +I+SWG+FR                       E PF+ +S  
Sbjct: 121  YIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERSSG 180

Query: 2290 KLSVELEFDASLVPFYVSFLLKAPFSGE--KLEIRSHRKTNFCFPVGIRSGYPAPLGLSY 2117
              +++L+F+A+  PFY+SFLLK+    +    +IRSHRKTNFC PVG   GYPAPLGLS+
Sbjct: 181  SFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLSF 240

Query: 2116 TADGSINFALFSRNAKSVILCLFDDSSLEEPTLEIDLDPYINRSGDIWHASMDSAMNFVS 1937
            ++DGS NFA FSRNA  V+LCL+D +S ++P LEIDLDPY+NR+GDIWHASM+S  +FVS
Sbjct: 241  SSDGSPNFAFFSRNAGGVVLCLYDGTS-DKPALEIDLDPYVNRTGDIWHASMESVGSFVS 299

Query: 1936 YGYRCRNG-AQDNSQA---SCILLDPYAKVIGENF-----------LGEICSELAFDWSG 1802
            YGYRC+    QD+ +      + LDPYAK+I  +F           LGE+  E AF+W+ 
Sbjct: 300  YGYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGELQKEPAFNWND 359

Query: 1801 DVHPRLSMEKLMVYRLNVMDFTKDASSNLPNEIRGTFSGVTEKLHHFKDLGVNAILLEPI 1622
            DVHP + MEKL+VYRLNVM FTKD SS + +++ GTFSG+ EKLHHFKDLGVNA+LLEPI
Sbjct: 360  DVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPI 419

Query: 1621 VPFDKQVGPYFPCHFFSPQNSFGPHGGSTSTIKSMKEMVKRLHANGIEILLEVVLTHTCD 1442
              FD+Q GPYFP HFFSP N +GP  G  STI S+KEMVKRLHANGIE+LLEVV THT +
Sbjct: 420  FSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTHTAE 479

Query: 1441 DAPLTKIDRPSYYLGKEGDNLATKNSLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINAS 1262
               L  ID   YY      +L  +N+LNC + +VQQ+I+DSLR+WV E+H+DGFCFINAS
Sbjct: 480  SGALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVDGFCFINAS 539

Query: 1261 SMLRGFNGENLSRPPLIEAIAFDPILSKTKIIADSFDPFHKSAKEIQFPHWKRWAEMNTK 1082
            S+LRGF+GE LSRPPL+E IAFDP+LSKTKIIAD +DP +   KEI+FPHWKRWAE+NT+
Sbjct: 540  SLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWKRWAEVNTR 599

Query: 1081 FCSDARNYLRGKTLLSNLATRLCGSGDIFLNGRGPAFSFNFVARNHGLSLVDLVSFSS-- 908
            FC+D RN+LRG+  LS+ ATRLCGSGDIF++GRGPAFSFNF  +N GL LVDLVSFSS  
Sbjct: 600  FCNDVRNFLRGEG-LSDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVDLVSFSSSE 658

Query: 907  -ATELSWNCGEEGATKKKGVLETRLKQIRNFLFILYVSLGVPVLNMGDECGRSSGGSLEH 731
             A+ELSWNCG+EG T K  VLE RLKQIRNFLFILYVSLGVP+LNMGDECG+SSGGS  +
Sbjct: 659  LASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQSSGGSPAY 718

Query: 730  SDRKPFNWKALETGFAIQTTQFISFLSALKIRRSDLLQSREFLKVENIDWHGTNLSPPDW 551
             DRKPFNW +++TGF IQT QFISFLS+L+ RRSDLLQ R FLK E+IDWHG++ SPP W
Sbjct: 719  GDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHGSDQSPPRW 778

Query: 550  EDPTSKFLAVSLXXXXXXXXDENQ---LNGDMFVAFNGGDDPMIATLPPPASNMVWLRLV 380
            +DP+SKFLA++L          ++   + GD+F+AFN  D  +   LPPP + MVW RLV
Sbjct: 779  DDPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGMVWHRLV 838

Query: 379  DTALRYPGFFSTAGDPTPEKIPGSPVYEMAPHSCVLLEARSVDG 248
            DTAL +PGFF+  G+   +K  G   Y+M  HSC L EA ++DG
Sbjct: 839  DTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEANTLDG 882


>gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  943 bits (2437), Expect = 0.0
 Identities = 487/876 (55%), Positives = 614/876 (70%), Gaps = 24/876 (2%)
 Frame = -3

Query: 2779 MAMLSLSSATQPPYLTCRTTNLSKLIVADSCKYGRRVARSLGRMDMERSLVFGEVAKNMI 2600
            MA   +  A     L+  +T  +KL+ + S   G+ V  SL ++++E     G    N  
Sbjct: 1    MATSPIQLAVHSRLLSYGSTESTKLVPSSSGNRGKIVC-SLRKLELEDMNFSGIGRNNDQ 59

Query: 2599 KGSCHDLGVGVLAASTLPVIQTVDKLSTYLFRTEKGGHVKVITRLKDDKYGVDVEVSSLQ 2420
            +          L+AS + ++ +  ++ TYLFRT+ GG VKV+    + KY V VEV  L+
Sbjct: 60   EAPRRAHRRKALSASRISLVPSAKRVPTYLFRTDIGGQVKVLVERTNGKYKVLVEVLPLE 119

Query: 2419 LSGNDDDVIMSWGIFRXXXXXXXXXXXXXSGI------FETPFVNKSLNKLSVELEFDAS 2258
            LS    +++M WG+FR              G        ETPFV     K++VEL+F+AS
Sbjct: 120  LSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEAS 179

Query: 2257 LVPFYVSFLLKAPFSG--EKLEIRSHRKTNFCFPVGIRSGYPAPLGLSYTADGSINFALF 2084
            L PFY+SF +K+      E  EIRSHR TNF  PVG+ SG+PAPLG+S+  DGS+NFALF
Sbjct: 180  LAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGSVNFALF 239

Query: 2083 SRNAKSVILCLFDDSSLEEPTLEIDLDPYINRSGDIWHASMDSAMNFVSYGYRCRNGAQD 1904
            SR+A+SV+LCL+DD S+E+P+LEIDLDPYINRSGDIWHA++D ++ F +YGYRC+  A  
Sbjct: 240  SRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRCK--ATT 297

Query: 1903 NSQASCILLDPYAKVIGE------------NFLGEICSELAFDWSGDVHPRLSMEKLMVY 1760
            + +   +LLDPYAKVI               +LGE+C E  +DWSGDV P L MEKL++Y
Sbjct: 298  SGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLIIY 357

Query: 1759 RLNVMDFTKDASSNLPNEIRGTFSGVTEKLHHFKDLGVNAILLEPIVPFDKQVGPYFPCH 1580
            RLNV  FTKD SS LP+++ GTFSG++EK HHFKDLGVNA+LLEPI PFD+Q GPYFP H
Sbjct: 358  RLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFPWH 417

Query: 1579 FFSPQNSFGPHGGSTSTIKSMKEMVKRLHANGIEILLEVVLTHTCDDAPLTKIDRPSYYL 1400
            FFSP N +GP G   S IKSMK+MVK+LHANGIE+ LEVV THT +DAPL  +D  SY +
Sbjct: 418  FFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFSYCI 477

Query: 1399 GKEGDNLATKNSLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMLRGFNGENLSRP 1220
             K G  L  +N+LNC +P+VQQ+ILD LRHWVIE+HIDGF F+NASS+LRGFNGE LSRP
Sbjct: 478  -KGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEILSRP 536

Query: 1219 PLIEAIAFDPILSKTKIIADSFDPFHKSAKEIQFPHWKRWAEMNTKFCSDARNYLRGKTL 1040
            PL+EAIAFDPILSK K+IAD+++P    +KE  FPHW+RWAE+N +FC D R++LRG+ L
Sbjct: 537  PLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHWRRWAEINMRFCDDIRDFLRGEGL 596

Query: 1039 LSNLATRLCGSGDIFLNGRGPAFSFNFVARNHGLSLVDLVSFSS---ATELSWNCGEEGA 869
            LSNLATRLCGSGDIF  GRGPAFSFN++ARN GL+LVDLVSFSS   A+ELSWNCG+EGA
Sbjct: 597  LSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSSNEVASELSWNCGQEGA 656

Query: 868  TKKKGVLETRLKQIRNFLFILYVSLGVPVLNMGDECGRSSGGSLEHSDRKPFNWKALETG 689
            T    VLE RLKQ+RNFLFIL++SLGVPVLNMGDECG+SSGG   +  RK   W  L+TG
Sbjct: 657  TTNNIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGPPAYDARKSLGWNTLKTG 716

Query: 688  FAIQTTQFISFLSALKIRRSDLLQSREFLKVENIDWHGTNLSPPDWEDPTSKFLAVSL-X 512
            F  Q  QFISFLS L++RRSDLLQ R FLK ENI WHG++ SPP W+ P+SKFLA++L  
Sbjct: 717  FGTQIAQFISFLSNLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDGPSSKFLAMTLKA 776

Query: 511  XXXXXXXDENQLNGDMFVAFNGGDDPMIATLPPPASNMVWLRLVDTALRYPGFFSTAGDP 332
                     + + GD+FVAFNG  D  I  LPPP ++MVW RLVDTAL +PGFF   G P
Sbjct: 777  DAEVSQTLVSDIVGDLFVAFNGAGDSEIVILPPPPTDMVWHRLVDTALPFPGFFDEKGTP 836

Query: 331  TPEKIPGSPVYEMAPHSCVLLEARSVDG*HSTYRKE 224
              +++     YEM  HSC+L EA+ +    S+ RK+
Sbjct: 837  VEDEL---VAYEMKSHSCLLFEAQRLAEIDSSKRKK 869


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  935 bits (2417), Expect = 0.0
 Identities = 480/877 (54%), Positives = 602/877 (68%), Gaps = 33/877 (3%)
 Frame = -3

Query: 2779 MAMLSLSSATQPPYLTCRTTNLSKLIVADSCKYGRRVARSLGRMDMERSLVFGEVAKNMI 2600
            MA +  S A +P Y  C  T  SKL V  +  +GR V   L +    + L F + + +  
Sbjct: 1    MATIIPSFAIRPCYCVCGATESSKLAVTGNKDFGRLV---LDQRLFNQRLHFWKQSPHWS 57

Query: 2599 KGS--CHDLGVGV----LAASTLPVIQTVDKLSTYLFRTEKGGHVKVITRLKDDKYGVDV 2438
              S  C    V V    L  ST   +  ++K+STYLFRT+ GGHVKV+ R K+ KY V +
Sbjct: 58   LDSRVCSAARVPVQQTELRFSTSAPLDELNKVSTYLFRTQFGGHVKVLVRKKNAKYAVYI 117

Query: 2437 EVSSLQLSGNDDDVIMSWGIFRXXXXXXXXXXXXXSGI----FETPFVNKSLNKLSVELE 2270
            EVSSL+L   D  +++ WGI+R                     +T  V  S    ++ELE
Sbjct: 118  EVSSLELGTTDYRLMLIWGIYRSDSSCFMPLDSQNFAPNARKMDTALVQNSFGTFALELE 177

Query: 2269 FDASLVPFYVSFLLKAPFSGEK--LEIRSHRKTNFCFPVGIRSGYPAPLGLSYTADGSIN 2096
            F+    PFY+SFLLK+  + +   LEI++H+  NFC P+G  SG P+PLGLS++ DGS+N
Sbjct: 178  FEPKQTPFYLSFLLKSKLNTDASGLEIKNHKNANFCVPIGFNSGDPSPLGLSFSTDGSMN 237

Query: 2095 FALFSRNAKSVILCLFDDSSLEEPTLEIDLDPYINRSGDIWHASMDSAMNFVSYGYRCRN 1916
            FA FSRN + ++LCL+DDS+ ++P LE+DLDPY+NR+GD+WHAS++ A  F SYGYRC+ 
Sbjct: 238  FAFFSRNVEGLVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASLEGAWTFTSYGYRCKG 297

Query: 1915 ----GAQDNSQASCILLDPYAKVIGEN-----------FLGEICSELAFDWSGDVHPRLS 1781
                G        C+LLDPYA+VI  +           +LG +C E AF+W  D+ P L+
Sbjct: 298  AILQGNTSKVDMECVLLDPYARVIASSMTDHGSRLSAKYLGRLCEEPAFEWGSDIRPNLA 357

Query: 1780 MEKLMVYRLNVMDFTKDASSNLPNEIRGTFSGVTEKLHHFKDLGVNAILLEPIVPFDKQV 1601
            MEKL+VYRLNV  FT+  S  L ++I GTF+G+ EK+ HF++LGVNA+LLEPI PFD+Q 
Sbjct: 358  MEKLIVYRLNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVLLEPIFPFDEQK 417

Query: 1600 GPYFPCHFFSPQNSFGPHGGSTSTIKSMKEMVKRLHANGIEILLEVVLTHTCDDAPLTKI 1421
            GPYFP HFFSP N +GP GGS S I SMKEMVK LHAN IE+LLEVV THT +   L  I
Sbjct: 418  GPYFPYHFFSPSNIYGPSGGSISAITSMKEMVKELHANRIEVLLEVVFTHTAEGGALQGI 477

Query: 1420 DRPSYYLGKEGDNLATKNSLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMLRGFN 1241
            D  SYY  K   ++ ++N+LNC +P+VQ++ILDSL+HWV E+HIDGFCFINAS++L GF+
Sbjct: 478  DDFSYYYTKS--SMDSRNALNCNYPIVQRMILDSLQHWVTEFHIDGFCFINASALLTGFH 535

Query: 1240 GENLSRPPLIEAIAFDPILSKTKIIADSFDPFHKSAKEIQFPHWKRWAEMNTKFCSDARN 1061
            GE+LSRPPL+EAIAFDPILSKTKIIAD + P H+  KE  FPHWKRWAE+N KFC D RN
Sbjct: 536  GEHLSRPPLVEAIAFDPILSKTKIIADPWHPEHRIPKETCFPHWKRWAEINPKFCIDVRN 595

Query: 1060 YLRGKTLLSNLATRLCGSGDIFLNGRGPAFSFNFVARNHGLSLVDLVSFSS---ATELSW 890
            +LRG++LL +LATRLCGSGDIF NGRGPAFSFN++ARN GL LVDLVSFS     +ELSW
Sbjct: 596  FLRGESLLGDLATRLCGSGDIFSNGRGPAFSFNYIARNSGLPLVDLVSFSGGELGSELSW 655

Query: 889  NCGEEGATKKKGVLETRLKQIRNFLFILYVSLGVPVLNMGDECGRSSGGSLEHSDRKPFN 710
            NCGEEG T K  VLE RLKQIRN+LFILYVSLGVPVLNMGDECG+SS GS+ + DRKPF+
Sbjct: 656  NCGEEGPTNKTAVLERRLKQIRNYLFILYVSLGVPVLNMGDECGQSSRGSISYGDRKPFD 715

Query: 709  WKALETGFAIQTTQFISFLSALKIRRSDLLQSREFLKVENIDWHGTNLSPPDWEDPTSKF 530
            W AL T F  Q TQFISFLS+L++RRSDLLQ R FLK ENIDWHG + SPP WEDPT KF
Sbjct: 716  WNALSTSFGNQMTQFISFLSSLRMRRSDLLQKRNFLKEENIDWHGNDQSPPRWEDPTCKF 775

Query: 529  LAVSLXXXXXXXXDENQ---LNGDMFVAFNGGDDPMIATLPPPASNMVWLRLVDTALRYP 359
            LA++L          ++   + GD+F+AFN         LPP    M+W RLVDTAL +P
Sbjct: 776  LAMTLKIDKAESQLSSEPSNIKGDLFMAFNAAGHAESVILPPVPEGMIWRRLVDTALPFP 835

Query: 358  GFFSTAGDPTPEKIPGSPVYEMAPHSCVLLEARSVDG 248
            GFFS  G+P  E+I G   Y+M  HSC L EA  +DG
Sbjct: 836  GFFSEDGEPVVEQIAGLIAYKMNSHSCTLFEAGIMDG 872


>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
          Length = 885

 Score =  897 bits (2317), Expect = 0.0
 Identities = 468/883 (53%), Positives = 590/883 (66%), Gaps = 45/883 (5%)
 Frame = -3

Query: 2764 LSSATQPPYLT--CRTTNLSKLIVADSCKYGRRVARSLGRMDMERSLVFGEVAKNMIKGS 2591
            LSS    P+    C  T   KL  +D   YG++     G+MD  R L  GE     +K S
Sbjct: 5    LSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSS 64

Query: 2590 CHDLGVGVLAASTLPVIQTVDKL------------STYLFRTEKGGHVKVITRLKDDKYG 2447
              +L     A S + V ++  +L            +TYLFRTE G  V V    K   + 
Sbjct: 65   HRNLSK-TYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGSTFT 123

Query: 2446 VDVEVSSLQLSGNDDDVIMSWGIFRXXXXXXXXXXXXXS-----GIFETPFVNKSLNKLS 2282
            V++EV S+QL   D+ +++SWG++R                   G  ETPFV  S  K S
Sbjct: 124  VNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEGKFS 183

Query: 2281 VELEFDASLVPFYVSFLLKAPFSGEK--LEIRSHRKTNFCFPVGIRSGYPAPLGLSYTAD 2108
            VELEFDA   PFY+SF+LK P   +    EIRSH+KT+F  PVG   GYP+PLGLS + D
Sbjct: 184  VELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSISGD 243

Query: 2107 GSINFALFSRNAKSVILCLFDDSSLEEPTLEIDLDPYINRSGDIWHASMDSAMNFVSYGY 1928
            GS+NF++FS +A+S++LCL++DS+ E+P LE+DLDPYINRSG+IWHAS + A  FVSYGY
Sbjct: 244  GSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGY 303

Query: 1927 RCRNGA----QDNSQASCILLDPYAKVIGEN--------------FLGEICSELAFDWSG 1802
            +C+       QD  + S I++DPYAK++  +              FLG+I     FDW G
Sbjct: 304  QCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDG 363

Query: 1801 DVHPRLSMEKLMVYRLNVMDFTKDASSNLPNEIRGTFSGVTEKLHHFKDLGVNAILLEPI 1622
            +VHP L MEKL VYRLNV  FT D SS LP +I GTFSG+T+KL HFK+LGVNA+LLEPI
Sbjct: 364  EVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPI 423

Query: 1621 VPFDKQVGPYFPCHFFSPQNSFGPHGGSTSTIKSMKEMVKRLHANGIEILLEVVLTHTCD 1442
              FD++ GPYFP HFFSP N++GP G S S I SMKEMVK LHANG+E++LEVV THT  
Sbjct: 424  FQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSG 483

Query: 1441 DAPLTKIDRPSYYLGKEGDNLATKNSLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINAS 1262
            +  L  ID  SYY      NL  K++LNC +P+VQQL+LDSLR+WV E+H+DGFCF+NAS
Sbjct: 484  NGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNAS 543

Query: 1261 SMLRGFNGENLSRPPLIEAIAFDPILSKTKIIADSFDPFHKSAKEIQFPHWKRWAEMNTK 1082
             +LRG +GE LSRPP +EAIAFDP+LSKTK++AD +DP    +KE +FPHWKRWAE+N+K
Sbjct: 544  FLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSK 603

Query: 1081 FCSDARNYLRGKTLLSNLATRLCGSGDIFLNGRGPAFSFNFVARNHGLSLVDLVSFSS-- 908
            FCSD R++ RG+ L+S+LATRLCGSGD+F +GRGPAFSFNF+ARN GL LVDLVSFS+  
Sbjct: 604  FCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSN 663

Query: 907  -ATELSWNCGEEGATKKKGVLETRLKQIRNFLFILYVSLGVPVLNMGDECGRSSGGSLEH 731
             A+ELSWNCGEEG T    VLE RLKQIRNF+F+L+VSLGVPVLNMGDECG+SSGGS+  
Sbjct: 664  LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAF 723

Query: 730  SDRKPFNWKALETGFAIQTTQFISFLSALKIRRSDLLQSREFLKVENIDWHGTNLSPPDW 551
            +D++ FNW  L+T F  QTTQFI+FLS+ + RR DL Q+R FLK ENIDW   N SPP W
Sbjct: 724  NDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQW 783

Query: 550  EDPTSKFLAVSL--XXXXXXXXDEN-QLNGDMFVAFNGGDDPMIATLPPPASNMVWLRLV 380
            ED + KFLAV L           EN +   ++F+ FN  D      LP P     W R+V
Sbjct: 784  EDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFRVV 843

Query: 379  DTALRYPGFFSTAGDPTPEKIPGSPVYEMAPHSCVLLEARSVD 251
            DTAL +PGFFS+ G+  P  + GS  YE+  HSC L EA+S +
Sbjct: 844  DTALPFPGFFSSDGELVP--MTGSVTYEIQAHSCALFEAKSAN 884


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Cucumis sativus]
          Length = 885

 Score =  896 bits (2316), Expect = 0.0
 Identities = 468/883 (53%), Positives = 590/883 (66%), Gaps = 45/883 (5%)
 Frame = -3

Query: 2764 LSSATQPPYLT--CRTTNLSKLIVADSCKYGRRVARSLGRMDMERSLVFGEVAKNMIKGS 2591
            LSS    P+    C  T   KL  +D   YG++     G+MD  R L  GE     +K S
Sbjct: 5    LSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSS 64

Query: 2590 CHDLGVGVLAASTLPVIQTVDKL------------STYLFRTEKGGHVKVITRLKDDKYG 2447
              +L     A S + V ++  +L            +TYLFRTE G  V V    K   + 
Sbjct: 65   HRNLSK-TYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGSTFT 123

Query: 2446 VDVEVSSLQLSGNDDDVIMSWGIFRXXXXXXXXXXXXXS-----GIFETPFVNKSLNKLS 2282
            V++EV S+QL   D+ +++SWG++R                   G  ETPFV  S  K S
Sbjct: 124  VNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEGKFS 183

Query: 2281 VELEFDASLVPFYVSFLLKAPFSGEK--LEIRSHRKTNFCFPVGIRSGYPAPLGLSYTAD 2108
            VELEFDA   PFY+SF+LK P   +    EIRSH+KT+F  PVG   GYP+PLGLS + D
Sbjct: 184  VELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSISGD 243

Query: 2107 GSINFALFSRNAKSVILCLFDDSSLEEPTLEIDLDPYINRSGDIWHASMDSAMNFVSYGY 1928
            GS+NF++FS +A+S++LCL++DS+ E+P LE+DLDPYINRSG+IWHAS + A  FVSYGY
Sbjct: 244  GSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGY 303

Query: 1927 RCRNGA----QDNSQASCILLDPYAKVIGEN--------------FLGEICSELAFDWSG 1802
            +C+       QD  + S I++DPYAK++  +              FLG+I     FDW G
Sbjct: 304  QCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDG 363

Query: 1801 DVHPRLSMEKLMVYRLNVMDFTKDASSNLPNEIRGTFSGVTEKLHHFKDLGVNAILLEPI 1622
            +VHP L MEKL VYRLNV  FT D SS LP +I GTFSG+T+KL HFK+LGVNA+LLEPI
Sbjct: 364  EVHPNLPMEKLXVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPI 423

Query: 1621 VPFDKQVGPYFPCHFFSPQNSFGPHGGSTSTIKSMKEMVKRLHANGIEILLEVVLTHTCD 1442
              FD++ GPYFP HFFSP N++GP G S S I SMKEMVK LHANG+E++LEVV THT  
Sbjct: 424  FQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSG 483

Query: 1441 DAPLTKIDRPSYYLGKEGDNLATKNSLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINAS 1262
            +  L  ID  SYY      NL  K++LNC +P+VQQL+LDSLR+WV E+H+DGFCF+NAS
Sbjct: 484  NGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNAS 543

Query: 1261 SMLRGFNGENLSRPPLIEAIAFDPILSKTKIIADSFDPFHKSAKEIQFPHWKRWAEMNTK 1082
             +LRG +GE LSRPP +EAIAFDP+LSKTK++AD +DP    +KE +FPHWKRWAE+N+K
Sbjct: 544  FLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSK 603

Query: 1081 FCSDARNYLRGKTLLSNLATRLCGSGDIFLNGRGPAFSFNFVARNHGLSLVDLVSFSS-- 908
            FCSD R++ RG+ L+S+LATRLCGSGD+F +GRGPAFSFNF+ARN GL LVDLVSFS+  
Sbjct: 604  FCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSN 663

Query: 907  -ATELSWNCGEEGATKKKGVLETRLKQIRNFLFILYVSLGVPVLNMGDECGRSSGGSLEH 731
             A+ELSWNCGEEG T    VLE RLKQIRNF+F+L+VSLGVPVLNMGDECG+SSGGS+  
Sbjct: 664  LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAF 723

Query: 730  SDRKPFNWKALETGFAIQTTQFISFLSALKIRRSDLLQSREFLKVENIDWHGTNLSPPDW 551
            +D++ FNW  L+T F  QTTQFI+FLS+ + RR DL Q+R FLK ENIDW   N SPP W
Sbjct: 724  NDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQW 783

Query: 550  EDPTSKFLAVSL--XXXXXXXXDEN-QLNGDMFVAFNGGDDPMIATLPPPASNMVWLRLV 380
            ED + KFLAV L           EN +   ++F+ FN  D      LP P     W R+V
Sbjct: 784  EDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFRVV 843

Query: 379  DTALRYPGFFSTAGDPTPEKIPGSPVYEMAPHSCVLLEARSVD 251
            DTAL +PGFFS+ G+  P  + GS  YE+  HSC L EA+S +
Sbjct: 844  DTALPFPGFFSSDGELVP--MTGSVTYEIQAHSCALFEAKSAN 884