BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00012694
         (1277 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAN15317.1| isoamylase isoform 1 [Solanum tuberosum]               608   e-172
ref|XP_004163012.1| PREDICTED: isoamylase 1, chloroplastic-like ...   580   e-163
ref|XP_004149676.1| PREDICTED: isoamylase 1, chloroplastic-like ...   580   e-163
ref|XP_002308090.1| predicted protein [Populus trichocarpa] gi|2...   580   e-163
gb|AAY84833.1| isoamylase [Ipomoea batatas]                           580   e-163

>gb|AAN15317.1| isoamylase isoform 1 [Solanum tuberosum]
          Length = 793

 Score =  608 bits (1569), Expect = e-172
 Identities = 297/409 (72%), Positives = 336/409 (82%), Gaps = 5/409 (1%)
 Frame = +3

Query: 66   QLRYASYSSIHTPKLT-STHNFDFLNPN-VQIKWRTRKNNNALMVNASDGGRGGEVDTAV 239
            +L +    S + PKL+   H F   + N V  +   RK+ ++++  A D GRGG V TA 
Sbjct: 2    ELLHCPSISTYKPKLSFHNHLFSRRSSNGVDFESIWRKSRSSVVNAAVDSGRGGVVKTAA 61

Query: 240  --VEKEKPKVSPRRFQVLQGSPTPFGATATKDGVNFAIYSRNATSATLCLMSPSDLPEKR 413
              V  EKP     RF+VL G P PFGATAT  GVNFA++SRNAT+ATLCL++ SDLPEKR
Sbjct: 62   TAVVVEKPTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSDLPEKR 121

Query: 414  VAEQIPLDQLTNKTGDVWHVFLKGDFTDMLYGYKFSGEFCPEEGHYYDSSQILLDPYAKA 593
            V EQI LD L NKTGDVWHVFLKGDF +MLYGYKF G+FCPEEGHY+DSSQI+LDPYAKA
Sbjct: 122  VTEQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLDPYAKA 181

Query: 594  VISRGEFGVLGPDDDCWSQMACQIPTIHE-FDWEGDLPLAFAQRDLVIYEMHVRGFTRHE 770
            ++SRGE+GVLGP+DDCW  MA  +P+  + FDWEGDLPL F QRDLVIYEMHVRGFT HE
Sbjct: 182  IVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFPQRDLVIYEMHVRGFTNHE 241

Query: 771  SSQTGSRGTYLGVVDKLDHLKELGVNCIELMPCHEFNELEYFSYNPVLGDYRLNYWGYST 950
            SS+T   GTYLGVV+KLDHLKELGVNCIELMPCHEFNELEY+SYN VLGDY+ N+WGYST
Sbjct: 242  SSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYST 301

Query: 951  INYFSPMLRYASAGARNCGLDAINEFKQLVKAAHKRGIEVLMDVVFNHTAEGNENGPILS 1130
            +N+FSPM RY+SAG  NCGL AINEFK LVK AHKRGIEV+MDVVFNHTAEGNENGPILS
Sbjct: 302  VNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPILS 361

Query: 1131 FRGIDNSVFYMLAPKGEFYNYSGCGNTFNCNHPVVRQFIVDSLRYWVTE 1277
            FRGIDNSVFY LAPKGEFYNYSGCGNTFNCN+P+VRQFIVD LRYWVTE
Sbjct: 362  FRGIDNSVFYTLAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCLRYWVTE 410


>ref|XP_004163012.1| PREDICTED: isoamylase 1, chloroplastic-like [Cucumis sativus]
          Length = 810

 Score =  580 bits (1495), Expect = e-163
 Identities = 271/360 (75%), Positives = 311/360 (86%), Gaps = 5/360 (1%)
 Frame = +3

Query: 213  RGGEVDTAVVEKEKPKVSP-RRFQVLQGSPTPFGATATKDGVNFAIYSRNATSATLCLMS 389
            RG EV T+V   E+ +VS   RF+V  G+PTPFGATA +DG+NFAIYS NA SATLCL+ 
Sbjct: 65   RGAEVRTSVAVAEEVEVSNLNRFEVFDGTPTPFGATAREDGINFAIYSANAVSATLCLIH 124

Query: 390  PSDLPEKRVAEQIPLDQLTNKTGDVWHVFLKGDFTDMLYGYKFSGEFCPEEGHYYDSSQI 569
             SDL E RV ++I LD LTNKTGDVWHVFLKGDFT+MLYGYKF G+F P+EG Y+DSS+I
Sbjct: 125  LSDLQENRVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDSSRI 184

Query: 570  LLDPYAKAVISRGEFGVLGPDDDCWSQMACQIPTIHE----FDWEGDLPLAFAQRDLVIY 737
            LLDPYAKAVISRGEFG LGP+D+CW QMA  +PT +E    FDWEGDLP+  AQ+DL+IY
Sbjct: 185  LLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLPVQHAQKDLIIY 244

Query: 738  EMHVRGFTRHESSQTGSRGTYLGVVDKLDHLKELGVNCIELMPCHEFNELEYFSYNPVLG 917
            EMHVRGFTRHESSQ+   GTYLG+V+KLD+LKELG+NCIELMPCHEFNELEYFSYN VLG
Sbjct: 245  EMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNELEYFSYNSVLG 304

Query: 918  DYRLNYWGYSTINYFSPMLRYASAGARNCGLDAINEFKQLVKAAHKRGIEVLMDVVFNHT 1097
            DY++N+WGYST+NYFSPM+RY+SAG  NCG DAINEFK L++ AHKRGIEV+MDVVFNHT
Sbjct: 305  DYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHT 364

Query: 1098 AEGNENGPILSFRGIDNSVFYMLAPKGEFYNYSGCGNTFNCNHPVVRQFIVDSLRYWVTE 1277
            AEGNENGP +SFRG+DN+V+YMLAP GEFYNYSGCGNTFNCNHP VR FIVD LRYWVTE
Sbjct: 365  AEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDCLRYWVTE 424


>ref|XP_004149676.1| PREDICTED: isoamylase 1, chloroplastic-like [Cucumis sativus]
          Length = 810

 Score =  580 bits (1495), Expect = e-163
 Identities = 271/360 (75%), Positives = 311/360 (86%), Gaps = 5/360 (1%)
 Frame = +3

Query: 213  RGGEVDTAVVEKEKPKVSP-RRFQVLQGSPTPFGATATKDGVNFAIYSRNATSATLCLMS 389
            RG EV T+V   E+ +VS   RF+V  G+PTPFGATA +DG+NFAIYS NA SATLCL+ 
Sbjct: 65   RGAEVRTSVAVAEEVEVSNLNRFEVFDGTPTPFGATAREDGINFAIYSANAVSATLCLIH 124

Query: 390  PSDLPEKRVAEQIPLDQLTNKTGDVWHVFLKGDFTDMLYGYKFSGEFCPEEGHYYDSSQI 569
             SDL E RV ++I LD LTNKTGDVWHVFLKGDFT+MLYGYKF G+F P+EG Y+DSS+I
Sbjct: 125  LSDLQENRVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDSSRI 184

Query: 570  LLDPYAKAVISRGEFGVLGPDDDCWSQMACQIPTIHE----FDWEGDLPLAFAQRDLVIY 737
            LLDPYAKAVISRGEFG LGP+D+CW QMA  +PT +E    FDWEGDLP+  AQ+DL+IY
Sbjct: 185  LLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLPVQHAQKDLIIY 244

Query: 738  EMHVRGFTRHESSQTGSRGTYLGVVDKLDHLKELGVNCIELMPCHEFNELEYFSYNPVLG 917
            EMHVRGFTRHESSQ+   GTYLG+V+KLD+LKELG+NCIELMPCHEFNELEYFSYN VLG
Sbjct: 245  EMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNELEYFSYNSVLG 304

Query: 918  DYRLNYWGYSTINYFSPMLRYASAGARNCGLDAINEFKQLVKAAHKRGIEVLMDVVFNHT 1097
            DY++N+WGYST+NYFSPM+RY+SAG  NCG DAINEFK L++ AHKRGIEV+MDVVFNHT
Sbjct: 305  DYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHT 364

Query: 1098 AEGNENGPILSFRGIDNSVFYMLAPKGEFYNYSGCGNTFNCNHPVVRQFIVDSLRYWVTE 1277
            AEGNENGP +SFRG+DN+V+YMLAP GEFYNYSGCGNTFNCNHP VR FIVD LRYWVTE
Sbjct: 365  AEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDCLRYWVTE 424


>ref|XP_002308090.1| predicted protein [Populus trichocarpa] gi|222854066|gb|EEE91613.1|
            predicted protein [Populus trichocarpa]
          Length = 826

 Score =  580 bits (1494), Expect = e-163
 Identities = 277/395 (70%), Positives = 318/395 (80%), Gaps = 1/395 (0%)
 Frame = +3

Query: 96   HTPKLTSTHNFDFLNPNVQIKWRTRKNNNALMVNASDGGRGGEVDTAVVEKEKPKVSPRR 275
            + PK  +T N + +  N+        +N+  ++       G +    VVE+E+PK+  ++
Sbjct: 27   YPPKRVTTSNLETIGSNIF-------SNSTALIPIKAASEGVDTAVVVVEEEEPKL--KK 77

Query: 276  FQVLQGSPTPFGATATKDGVNFAIYSRNATSATLCLMSPSDLPEKRVAEQIPLDQLTNKT 455
             QV +G P PFGAT    GVNFAI+S +A SATLCL+S SDLPE RV EQI LD LTNKT
Sbjct: 78   IQVFEGHPAPFGATVRDGGVNFAIFSADAVSATLCLISLSDLPENRVTEQIFLDPLTNKT 137

Query: 456  GDVWHVFLKGDFTDMLYGYKFSGEFCPEEGHYYDSSQILLDPYAKAVISRGEFGVLGPDD 635
            GDVWHV LKGDF DMLYGYKF G F PE G YYD S+I+LDPYAK+VISRGEFGVLG DD
Sbjct: 138  GDVWHVLLKGDFKDMLYGYKFDGNFSPEVGLYYDPSKIVLDPYAKSVISRGEFGVLGHDD 197

Query: 636  DCWSQMACQIPTI-HEFDWEGDLPLAFAQRDLVIYEMHVRGFTRHESSQTGSRGTYLGVV 812
            + W QMAC IPT  ++FDWEGD PL   QRDL+IYEMHVRGFT+HESS+T   GTYLGVV
Sbjct: 198  NRWPQMACMIPTAENKFDWEGDSPLKHPQRDLIIYEMHVRGFTQHESSRTEFPGTYLGVV 257

Query: 813  DKLDHLKELGVNCIELMPCHEFNELEYFSYNPVLGDYRLNYWGYSTINYFSPMLRYASAG 992
            +KLDHLKELGVNCIELMPCHEFNELEY+SYN VLGDY++N+WGYST+NYFSPM RY+SAG
Sbjct: 258  EKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKVNFWGYSTVNYFSPMTRYSSAG 317

Query: 993  ARNCGLDAINEFKQLVKAAHKRGIEVLMDVVFNHTAEGNENGPILSFRGIDNSVFYMLAP 1172
             RNCG DAINEFK LV+ AHKRGIEV MDVVFNHTAEGNE GPILSFRG+DNS++YMLAP
Sbjct: 318  TRNCGRDAINEFKLLVREAHKRGIEVFMDVVFNHTAEGNEKGPILSFRGVDNSIYYMLAP 377

Query: 1173 KGEFYNYSGCGNTFNCNHPVVRQFIVDSLRYWVTE 1277
            KGEFYNYSGCGNTFNCNHP+VRQFI+D LRYWVTE
Sbjct: 378  KGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTE 412


>gb|AAY84833.1| isoamylase [Ipomoea batatas]
          Length = 785

 Score =  580 bits (1494), Expect = e-163
 Identities = 282/413 (68%), Positives = 328/413 (79%), Gaps = 9/413 (2%)
 Frame = +3

Query: 66   QLRYASYSSIHTPKLTSTHN--------FDFLNPNVQIKWRTRKNNNALMVNASDGGRGG 221
            +L ++   S ++PKL S  +        F F + N   ++  +   +AL VNA+  G GG
Sbjct: 2    ELLHSPSISQYSPKLHSRSSNIRKTGLSFGFPH-NYFAEFEKKARPSALAVNAAIDG-GG 59

Query: 222  EVDTAVV-EKEKPKVSPRRFQVLQGSPTPFGATATKDGVNFAIYSRNATSATLCLMSPSD 398
            E DTAVV EK  P    RRF+VL G P PFGATA   G+NFA++S NATSA LCL+S +D
Sbjct: 60   ESDTAVVVEKPLPYGLLRRFEVLSGHPAPFGATARDGGINFAVFSSNATSAALCLISLAD 119

Query: 399  LPEKRVAEQIPLDQLTNKTGDVWHVFLKGDFTDMLYGYKFSGEFCPEEGHYYDSSQILLD 578
            LPEK+V EQIPLD   NKTGDVWHVFL+GDF +MLYGY F G+F PEEGHY+DS++ILLD
Sbjct: 120  LPEKKVTEQIPLDPSINKTGDVWHVFLQGDFDNMLYGYSFDGKFAPEEGHYFDSARILLD 179

Query: 579  PYAKAVISRGEFGVLGPDDDCWSQMACQIPTIHEFDWEGDLPLAFAQRDLVIYEMHVRGF 758
            PYAKA++SR EFG LGP+ DCW  MAC +PT  +FDWEGDLPL F QRDLVIYEMHVRGF
Sbjct: 180  PYAKAIVSRAEFGALGPEKDCWPPMACMLPTADKFDWEGDLPLKFPQRDLVIYEMHVRGF 239

Query: 759  TRHESSQTGSRGTYLGVVDKLDHLKELGVNCIELMPCHEFNELEYFSYNPVLGDYRLNYW 938
            T HESS T   GTY GVV+KLDHLK LGVNCIELMPCHEFNELEY+SYNPVLGDY++N+W
Sbjct: 240  TNHESSGTEFPGTYRGVVEKLDHLKVLGVNCIELMPCHEFNELEYYSYNPVLGDYKVNFW 299

Query: 939  GYSTINYFSPMLRYASAGARNCGLDAINEFKQLVKAAHKRGIEVLMDVVFNHTAEGNENG 1118
            GYST+N+FSPM RY+SAG    GL AI+EFK LV+ AH+RGIEV+MDVVFNHTAEGNENG
Sbjct: 300  GYSTVNFFSPMGRYSSAGMHKSGLGAIDEFKYLVREAHRRGIEVIMDVVFNHTAEGNENG 359

Query: 1119 PILSFRGIDNSVFYMLAPKGEFYNYSGCGNTFNCNHPVVRQFIVDSLRYWVTE 1277
            P+ SFRG+DNSVFYMLAPKGEFYNYSGCGNTFNCNHPV RQFI++ LRYWV E
Sbjct: 360  PMFSFRGVDNSVFYMLAPKGEFYNYSGCGNTFNCNHPVARQFILEFLRYWVIE 412