BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00011660
         (1722 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281338.1| PREDICTED: cytochrome P450 85A [Vitis vinife...   784   0.0  
gb|ABB60086.1| brassinosteroid-6-oxidase [Vitis vinifera]             782   0.0  
emb|CAN66537.1| hypothetical protein VITISV_029635 [Vitis vinifera]   764   0.0  
gb|ABG36709.1| cytochrome P450 CYP85A1 [Nicotiana tabacum]            762   0.0  
gb|ACR20476.1| steroid C-6 oxidase [Gossypium hirsutum]               759   0.0  

>ref|XP_002281338.1| PREDICTED: cytochrome P450 85A [Vitis vinifera]
            gi|298204461|emb|CBI16941.3| unnamed protein product
            [Vitis vinifera]
          Length = 464

 Score =  784 bits (2024), Expect = 0.0
 Identities = 378/462 (81%), Positives = 412/462 (89%)
 Frame = -2

Query: 1646 MAVFTXXXXXXXXXXXLSTALLRWNEMKYKKKGLPPGTMGWPLFGETTDFLKQGPSFMKN 1467
            MAVF            + TALLRWNE++Y+KKGLPPGTMGWP+FGETT+FLKQGPSFMKN
Sbjct: 1    MAVFMAVFGVVLIGLCICTALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKN 60

Query: 1466 QRARYGSVFKSHILGCPTIVSMDPELNRYILMNEGKGILPGYPQSMLDILGKSNIAAVHG 1287
            QRARYG  FKSH+LGCPT VSMDPELNRYILMNE KG++PGYPQSMLDILGK NIAAVHG
Sbjct: 61   QRARYGKFFKSHLLGCPTTVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHG 120

Query: 1286 PAHKNMRGALLSLVSPTIIRHQILPKIDDFIRSHLCNWTHQVVDIQEKTKEMALLSSLKQ 1107
              HK MRGALL+L+SPT+IR Q+LPKID+F+RSHL  W  ++++IQEKTKEMALLSSLKQ
Sbjct: 121  STHKYMRGALLALISPTMIRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEMALLSSLKQ 180

Query: 1106 ISGIESCSFSKQFMPEFFKLVLGTLSLPINLPNTNYHRGLQARKNIVKMLEQLIEERRNS 927
            I+GIES + SK+FMPEFFKLVLGT+SLPI+LP TNY RG QARKNIV ML QLIEER+ S
Sbjct: 181  IAGIESGTISKEFMPEFFKLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKAS 240

Query: 926  QEIHQDMLGLLMSGVENRYKLSDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPRVLE 747
            QE H DMLG LM   ENRYKLSDEEIID IITILYSGYETVSTTSMMAVKYLHDHPRVL+
Sbjct: 241  QETHNDMLGCLMRTNENRYKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLD 300

Query: 746  ELRKEHLGIRERKMAEDPLDWNDYKSMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGF 567
            ELRKEHL IRERK  EDP+DWNDYK M+FTRAVIFETSRLATIVNGVLRKTTKDMELNGF
Sbjct: 301  ELRKEHLAIRERKRPEDPIDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGF 360

Query: 566  IIPEGWRIYVYTREINYDPCLYPDPYTFNPWRWLDRSLETQNYFFIFGGGTRQCPGKELG 387
            +IP+GWRIYVYTREINYDP LYPDP  FNPWRWLD+SLE+QNYF +FGGGTRQCPGKELG
Sbjct: 361  VIPKGWRIYVYTREINYDPLLYPDPLAFNPWRWLDKSLESQNYFLLFGGGTRQCPGKELG 420

Query: 386  IVEISTFLHYFVTKYRWEEVGGDKLMKFPRVEAPNGLHIRVS 261
            I EISTFLHYFVT+YRWEEVGGDKLMKFPRVEAPNGLHIRVS
Sbjct: 421  IAEISTFLHYFVTRYRWEEVGGDKLMKFPRVEAPNGLHIRVS 462


>gb|ABB60086.1| brassinosteroid-6-oxidase [Vitis vinifera]
          Length = 460

 Score =  782 bits (2019), Expect = 0.0
 Identities = 374/444 (84%), Positives = 407/444 (91%)
 Frame = -2

Query: 1592 TALLRWNEMKYKKKGLPPGTMGWPLFGETTDFLKQGPSFMKNQRARYGSVFKSHILGCPT 1413
            TALLRWNE++Y+KKGLPPGTMGWP+FGETT+FLKQGPSFMKNQRARYG  FKSH+LGCPT
Sbjct: 15   TALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKNQRARYGKFFKSHLLGCPT 74

Query: 1412 IVSMDPELNRYILMNEGKGILPGYPQSMLDILGKSNIAAVHGPAHKNMRGALLSLVSPTI 1233
             VSMDPELNRYILMNE KG++PGYPQSMLDILGK NIAAVHG  HK MRGALL+L+SPT+
Sbjct: 75   TVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLALISPTM 134

Query: 1232 IRHQILPKIDDFIRSHLCNWTHQVVDIQEKTKEMALLSSLKQISGIESCSFSKQFMPEFF 1053
            IR Q+LPKID+F+RSHL  W  ++++IQEKTKEMALLSSLKQI+GIES + SK+FMPEFF
Sbjct: 135  IRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEMALLSSLKQIAGIESGTISKEFMPEFF 194

Query: 1052 KLVLGTLSLPINLPNTNYHRGLQARKNIVKMLEQLIEERRNSQEIHQDMLGLLMSGVENR 873
            KLVLGT+SLPI+LP TNY RG QARKNIV ML QLIEER+ SQE H DMLG LM   ENR
Sbjct: 195  KLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKASQETHNDMLGCLMRTNENR 254

Query: 872  YKLSDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPRVLEELRKEHLGIRERKMAEDP 693
            YKLSDEEIID IITILYSGYETVSTTSMMAVKYLHDHPRVL+ELRKEHL IRERK  EDP
Sbjct: 255  YKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLDELRKEHLAIRERKRPEDP 314

Query: 692  LDWNDYKSMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFIIPEGWRIYVYTREINYD 513
            +DWNDYK M+FTRAVIFETSRLATIVNGVLRKTTKDMELNGF+IP+GWRIYVYTREINYD
Sbjct: 315  IDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGFVIPKGWRIYVYTREINYD 374

Query: 512  PCLYPDPYTFNPWRWLDRSLETQNYFFIFGGGTRQCPGKELGIVEISTFLHYFVTKYRWE 333
            P LYPDP  FNPWRWLD+SLE+QNYF +FGGGTRQCPGKELGI EISTFLHYFVT+YRWE
Sbjct: 375  PLLYPDPLAFNPWRWLDKSLESQNYFLLFGGGTRQCPGKELGIAEISTFLHYFVTRYRWE 434

Query: 332  EVGGDKLMKFPRVEAPNGLHIRVS 261
            EVGGDKLMKFPRVEAPNGLHIRVS
Sbjct: 435  EVGGDKLMKFPRVEAPNGLHIRVS 458


>emb|CAN66537.1| hypothetical protein VITISV_029635 [Vitis vinifera]
          Length = 463

 Score =  764 bits (1972), Expect = 0.0
 Identities = 366/447 (81%), Positives = 402/447 (89%), Gaps = 3/447 (0%)
 Frame = -2

Query: 1592 TALLRWNEMKYKKKGLPPGTMGWPLFGETTDFLKQGPSFMKNQRARYGSVFKSHILGCPT 1413
            TALLRWNE++Y+KKGLPPGTMGWP+FGETT+FLKQGPSFMKNQRARYG  FKSH+LGCPT
Sbjct: 15   TALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKNQRARYGKFFKSHLLGCPT 74

Query: 1412 IVSMDPELNRYILMNEGKGILPGYPQSMLDILGKSNIAAVHGPAHKNMRGALLSLVSPTI 1233
             VSMDPELNRYILMNE KG++PGYPQSMLDILGK NIAAVHG  HK MRGALL+L+SPT+
Sbjct: 75   TVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLALISPTM 134

Query: 1232 IRHQILPKIDDFIRSHLCNWTHQVVDIQEKTKEM---ALLSSLKQISGIESCSFSKQFMP 1062
            IR Q+LPKID+F+RSHL  W  ++++IQEKTKE+   +L S    ++GIES + SK+FMP
Sbjct: 135  IRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEVYGTSLFSQADAVAGIESGTISKEFMP 194

Query: 1061 EFFKLVLGTLSLPINLPNTNYHRGLQARKNIVKMLEQLIEERRNSQEIHQDMLGLLMSGV 882
            EFFKLVLGT+SLPI+LP TNY RG QARKNIV ML QLIEER+ SQE H DMLG LM   
Sbjct: 195  EFFKLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKASQETHNDMLGCLMRTN 254

Query: 881  ENRYKLSDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPRVLEELRKEHLGIRERKMA 702
            ENRYKLSDEEIID IITILYSGYETVSTTSMMAVKYLHDHPRVL+ELRKEHL IRERK  
Sbjct: 255  ENRYKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLDELRKEHLAIRERKRP 314

Query: 701  EDPLDWNDYKSMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFIIPEGWRIYVYTREI 522
            EDP+DWNDYK M+FTRAVIFETSRLATIVNGVLRKTTKDMELNGF+IP+GWRIYVYTREI
Sbjct: 315  EDPIDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGFVIPKGWRIYVYTREI 374

Query: 521  NYDPCLYPDPYTFNPWRWLDRSLETQNYFFIFGGGTRQCPGKELGIVEISTFLHYFVTKY 342
            NYDP LYPDP  FNPWRWLD+SLE+QNYF +FGGGTRQCPGKELGI EISTFLHYFVT+Y
Sbjct: 375  NYDPLLYPDPLAFNPWRWLDKSLESQNYFLLFGGGTRQCPGKELGIAEISTFLHYFVTRY 434

Query: 341  RWEEVGGDKLMKFPRVEAPNGLHIRVS 261
            RWEEVGGDKLMKFPRVEAPNGLHIRVS
Sbjct: 435  RWEEVGGDKLMKFPRVEAPNGLHIRVS 461


>gb|ABG36709.1| cytochrome P450 CYP85A1 [Nicotiana tabacum]
          Length = 464

 Score =  762 bits (1967), Expect = 0.0
 Identities = 365/445 (82%), Positives = 405/445 (91%)
 Frame = -2

Query: 1595 STALLRWNEMKYKKKGLPPGTMGWPLFGETTDFLKQGPSFMKNQRARYGSVFKSHILGCP 1416
            STALLRWN++KY KK LPPGTMGWPLFGETT+FLKQGPSFMKNQRAR+GS FKSHILGCP
Sbjct: 18   STALLRWNQVKYNKKSLPPGTMGWPLFGETTEFLKQGPSFMKNQRARFGSFFKSHILGCP 77

Query: 1415 TIVSMDPELNRYILMNEGKGILPGYPQSMLDILGKSNIAAVHGPAHKNMRGALLSLVSPT 1236
            TIVSMD ELNRYIL+NE KG++PGYPQSMLDILGK NIAAVHG AHK MRGALLSL+SPT
Sbjct: 78   TIVSMDSELNRYILVNEAKGLVPGYPQSMLDILGKCNIAAVHGSAHKYMRGALLSLISPT 137

Query: 1235 IIRHQILPKIDDFIRSHLCNWTHQVVDIQEKTKEMALLSSLKQISGIESCSFSKQFMPEF 1056
            +IR Q+LPKID+F+RSHL NW  +V+DIQEKT +MA LSSLKQI+GIES S +++FMPEF
Sbjct: 138  MIRDQLLPKIDEFMRSHLTNWDSKVIDIQEKTNKMAFLSSLKQIAGIESTSLAQEFMPEF 197

Query: 1055 FKLVLGTLSLPINLPNTNYHRGLQARKNIVKMLEQLIEERRNSQEIHQDMLGLLMSGVEN 876
            FKLVLGTLSLPINLPNTNY RGLQARK IV +L  LIEERR S+EI  DMLG LM+   N
Sbjct: 198  FKLVLGTLSLPINLPNTNYGRGLQARKIIVSLLRTLIEERRASKEIQHDMLGYLMNEEAN 257

Query: 875  RYKLSDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPRVLEELRKEHLGIRERKMAED 696
            RYKL+D+E+ID IITILYSGYETVSTTSMMAVKYLHDHP+VLEELRKEHL IRE+K  ED
Sbjct: 258  RYKLTDDEMIDLIITILYSGYETVSTTSMMAVKYLHDHPKVLEELRKEHLAIREKKKPED 317

Query: 695  PLDWNDYKSMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFIIPEGWRIYVYTREINY 516
            P+D+NDYK+M+FTRAVI ETSRLATIVNGVLRKTT+DME+NG+IIP+GWRIYVYTRE+NY
Sbjct: 318  PIDYNDYKAMRFTRAVILETSRLATIVNGVLRKTTQDMEINGYIIPKGWRIYVYTRELNY 377

Query: 515  DPCLYPDPYTFNPWRWLDRSLETQNYFFIFGGGTRQCPGKELGIVEISTFLHYFVTKYRW 336
            DP LYPDPY FNPWRWLD+SLE QN F +FGGGTRQCPGKELG+ EISTFLHYFVTKY+W
Sbjct: 378  DPRLYPDPYAFNPWRWLDKSLENQNSFLVFGGGTRQCPGKELGVAEISTFLHYFVTKYKW 437

Query: 335  EEVGGDKLMKFPRVEAPNGLHIRVS 261
            EEVGGDKLMKFPRVEAPNGL IRVS
Sbjct: 438  EEVGGDKLMKFPRVEAPNGLRIRVS 462


>gb|ACR20476.1| steroid C-6 oxidase [Gossypium hirsutum]
          Length = 465

 Score =  759 bits (1960), Expect = 0.0
 Identities = 360/446 (80%), Positives = 410/446 (91%), Gaps = 1/446 (0%)
 Frame = -2

Query: 1592 TALLRWNEMKYKKKGLPPGTMGWPLFGETTDFLKQGPSFMKNQRARYGSVFKSHILGCPT 1413
            TA+LRWNEM+Y+KKGLPPGTMGWP+FGETT+FLKQGP+FMKNQRARYGS FKSHILGCPT
Sbjct: 19   TAVLRWNEMRYRKKGLPPGTMGWPVFGETTEFLKQGPNFMKNQRARYGSFFKSHILGCPT 78

Query: 1412 IVSMDPELNRYILMNEGKGILPGYPQSMLDILGKSNIAAVHGPAHKNMRGALLSLVSPTI 1233
            +VSMDPELNRYILMNE KG++PGYPQSMLDILGK NIAAVHG  HK MRGALL+L+SPT+
Sbjct: 79   VVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKQMRGALLALISPTM 138

Query: 1232 IRHQILPKIDDFIRSHLCNWTHQVVDIQEKTKEMALLSSLKQISGIESCSFSKQFMPEFF 1053
            IR Q+LPKID+F+R+ L NW +QV+DIQ++TKEMA LSSLKQI+  ES S ++QFMPEFF
Sbjct: 139  IRQQLLPKIDEFMRAFLSNWDNQVIDIQDRTKEMAFLSSLKQIASAESSSVAQQFMPEFF 198

Query: 1052 KLVLGTLSLPINLPNTNYHRGLQARKNIVKMLEQLIEERRNSQEIHQDMLGLLMSGVEN- 876
            KLVLGTLSL I+LP TNY RG QAR+NI+K+L QLIE+RR+S+E H+DMLG LM   +N 
Sbjct: 199  KLVLGTLSLHIDLPGTNYRRGFQARQNILKILGQLIEKRRDSEESHKDMLGYLMRNDDNN 258

Query: 875  RYKLSDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPRVLEELRKEHLGIRERKMAED 696
            R+KLSD+EIIDQIITILYSGYETVSTTSMMAVKYLHDHP VLEELRKEH+GIRERK A++
Sbjct: 259  RHKLSDDEIIDQIITILYSGYETVSTTSMMAVKYLHDHPTVLEELRKEHMGIRERKRADE 318

Query: 695  PLDWNDYKSMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFIIPEGWRIYVYTREINY 516
            P++WND KSM+FTRAVIFETSRLATIVNGVLRKTT+DMELNG++IP+GWRIYVYTREINY
Sbjct: 319  PIEWNDLKSMRFTRAVIFETSRLATIVNGVLRKTTQDMELNGYVIPKGWRIYVYTREINY 378

Query: 515  DPCLYPDPYTFNPWRWLDRSLETQNYFFIFGGGTRQCPGKELGIVEISTFLHYFVTKYRW 336
            DP LYPDP  FNPWRW+ + LE+QNYF IFGGGTRQCPGKELGI EISTFLHYFVT+YRW
Sbjct: 379  DPFLYPDPLAFNPWRWMVKGLESQNYFLIFGGGTRQCPGKELGIAEISTFLHYFVTRYRW 438

Query: 335  EEVGGDKLMKFPRVEAPNGLHIRVSN 258
            EEVGG+KLMKFPRVEAPNGLHIRVS+
Sbjct: 439  EEVGGEKLMKFPRVEAPNGLHIRVSS 464