BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00011446
         (1347 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADP00412.1| putative methyltransferase [Catharanthus roseus]       297   e-104
gb|ABI54118.1| caffeic acid O-methyltransferase [Malus x domestica]   298   e-103
dbj|BAC78827.1| caffeic acid O-methyltransferase [Rosa chinensis...   298   e-103
sp|Q8GU25.1|COMT1_ROSCH RecName: Full=Caffeic acid 3-O-methyltra...   298   e-103
gb|AFK39235.1| unknown [Medicago truncatula]                          293   e-102

>gb|ADP00412.1| putative methyltransferase [Catharanthus roseus]
          Length = 364

 Score =  297 bits (761), Expect(2) = e-104
 Identities = 145/255 (56%), Positives = 196/255 (76%), Gaps = 1/255 (0%)
 Frame = +1

Query: 109 NKMSNEEEGLLRVMQLNTRIVLPMLIKTAIELDLFEIMAKT-PGAQFSSYDLASNLPRQS 285
           N +SNE+E  LRVMQL++ ++LPM++KT IELDLFE+MAK  P AQ S+ +++S LP ++
Sbjct: 11  NIISNEKEDFLRVMQLSSGLILPMILKTVIELDLFELMAKAGPNAQLSAIEISSLLPTKN 70

Query: 286 PQTPVLIERILRFLASQSILASTLVTDERGNSKTLYGMTPMSNNFVRNQDGTSLASSLLL 465
           P+ PV+++RIL+FLAS S L   ++ DE+G +KTLY   P+   F+ ++DG SLAS L+L
Sbjct: 71  PEAPVMLDRILKFLASFSFLNCNVIADEQGGAKTLYSSAPICKKFLPDEDGISLASLLML 130

Query: 466 IYDKVLVDCWYHLKDAVVEGGIPFDKAHGMNAFEYPAKDNRFNQVFNKSMYDNTKIVMKM 645
             DK   D WYHLKDA++EGGIPF +AHG +AFE P KD RF  VFNK+++++  +VMK 
Sbjct: 131 PVDKAHFDSWYHLKDAILEGGIPFIRAHGADAFEQPIKDTRFFDVFNKALHNHACMVMKR 190

Query: 646 ILEKYKGFEGVKELVDVGGGLGATLEIIVSKYPNIKGINFDLPHVIKDAAPCPGVEHVGG 825
           +LE YKGFE VKELVDVGGG G TL  IV+KYPNI+ IN+D+P V++ AAP  GVE V G
Sbjct: 191 LLEVYKGFEEVKELVDVGGGHGGTLSCIVAKYPNIRAINYDIPQVVECAAPRSGVEFVRG 250

Query: 826 DMFENVPKGDVIFMK 870
           +MFE++PKG+V+ MK
Sbjct: 251 NMFESIPKGEVMMMK 265



 Score =  109 bits (273), Expect(2) = e-104
 Identities = 47/102 (46%), Positives = 71/102 (69%)
 Frame = +2

Query: 941  WILHDWGDNYCIKLLKNCWAALPEYGKVVVVETIIPDPEHQHSATDVSQNAIGSDIIMLI 1120
            W+LHDW D++C+ +LKNC+ ALPE GK++V+E ++P+     +   +S+     DI ML 
Sbjct: 266  WVLHDWDDDHCVTILKNCYEALPENGKLIVIELVLPETP---NGDTISKIGYQFDINMLS 322

Query: 1121 ANPGGKERMAKEYDTLAKEAGFTSLKIVCRVSTFYIMEFYKN 1246
             N GGKER  KE++ LA +AGF S+K++CR    +++EFYKN
Sbjct: 323  VNTGGKERTEKEFEHLATQAGFASIKLICRADCDWVIEFYKN 364


>gb|ABI54118.1| caffeic acid O-methyltransferase [Malus x domestica]
          Length = 365

 Score =  298 bits (763), Expect(2) = e-103
 Identities = 152/251 (60%), Positives = 187/251 (74%), Gaps = 1/251 (0%)
 Frame = +1

Query: 121 NEEEGLLRVMQLNTRIVLPMLIKTAIELDLFEIMAKT-PGAQFSSYDLASNLPRQSPQTP 297
           ++EE  L  MQL T  +L M++K AIELDL EIMAK  PGA  S  DL+S LP ++P +P
Sbjct: 15  SDEEANLFAMQLATGSILHMVLKAAIELDLLEIMAKAGPGAFVSPADLSSQLPTKNPDSP 74

Query: 298 VLIERILRFLASQSILASTLVTDERGNSKTLYGMTPMSNNFVRNQDGTSLASSLLLIYDK 477
           V+++R+LR LAS SIL  +L T   G  + LYG+ P+     +N+DG S+ S  LLI DK
Sbjct: 75  VMLDRMLRLLASYSILTYSLRTLPDGKVERLYGLGPVCKFLTKNEDGASIGSLCLLIQDK 134

Query: 478 VLVDCWYHLKDAVVEGGIPFDKAHGMNAFEYPAKDNRFNQVFNKSMYDNTKIVMKMILEK 657
           VL++ WYHLKDAV+EGGIPF+KA+GM AFEY   + RFN+VFNK M DN+ I MK +LE 
Sbjct: 135 VLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTEPRFNKVFNKGMADNSTITMKKLLEN 194

Query: 658 YKGFEGVKELVDVGGGLGATLEIIVSKYPNIKGINFDLPHVIKDAAPCPGVEHVGGDMFE 837
           Y GFEG+  +VDVGGG GA L +IVSKYP+IKGINFDLPHVI+DA   PGVEHVGGDMF 
Sbjct: 195 YNGFEGLTSIVDVGGGTGAVLNMIVSKYPSIKGINFDLPHVIEDAPQYPGVEHVGGDMFV 254

Query: 838 NVPKGDVIFMK 870
           +VPKGD IFMK
Sbjct: 255 SVPKGDAIFMK 265



 Score =  104 bits (260), Expect(2) = e-103
 Identities = 50/101 (49%), Positives = 64/101 (63%)
 Frame = +2

Query: 941  WILHDWGDNYCIKLLKNCWAALPEYGKVVVVETIIPDPEHQHSATDVSQNAIGSDIIMLI 1120
            WI HDW D +C+K LKNC+AALP+ GKV+V E I+P       AT   +  + SD IML 
Sbjct: 266  WICHDWTDEHCLKFLKNCYAALPDNGKVIVAEYILPVAPDGSLAT---KEVVHSDAIMLA 322

Query: 1121 ANPGGKERMAKEYDTLAKEAGFTSLKIVCRVSTFYIMEFYK 1243
             NPGGKER  KE++ LAK +GF   ++VC     Y +EF K
Sbjct: 323  HNPGGKERTEKEFEALAKGSGFQGFRVVCSAFNTYAIEFLK 363


>dbj|BAC78827.1| caffeic acid O-methyltransferase [Rosa chinensis var. spontanea]
          Length = 365

 Score =  298 bits (763), Expect(2) = e-103
 Identities = 151/251 (60%), Positives = 189/251 (75%), Gaps = 1/251 (0%)
 Frame = +1

Query: 121 NEEEGLLRVMQLNTRIVLPMLIKTAIELDLFEIMAKT-PGAQFSSYDLASNLPRQSPQTP 297
           ++EE  L  MQL +  VLPM++K AIELDL EIMAK  PGA  S  DLAS LP ++P+ P
Sbjct: 15  SDEEANLFAMQLASASVLPMVLKAAIELDLLEIMAKAGPGAFLSPNDLASQLPTKNPEAP 74

Query: 298 VLIERILRFLASQSILASTLVTDERGNSKTLYGMTPMSNNFVRNQDGTSLASSLLLIYDK 477
           V+++R+LR LAS SIL  +L T   G  + LYG+ P+     +N+DG S+A+  L+  DK
Sbjct: 75  VMLDRMLRLLASYSILTYSLRTLPDGKVERLYGLGPVCKFLTKNEDGVSIAALCLMNQDK 134

Query: 478 VLVDCWYHLKDAVVEGGIPFDKAHGMNAFEYPAKDNRFNQVFNKSMYDNTKIVMKMILEK 657
           VLV+ WYHLKDAV++GGIPF+KA+GM AF+Y   D RFN+VFNK M D++ I MK ILE 
Sbjct: 135 VLVESWYHLKDAVLDGGIPFNKAYGMTAFDYHGTDPRFNKVFNKGMADHSTITMKKILET 194

Query: 658 YKGFEGVKELVDVGGGLGATLEIIVSKYPNIKGINFDLPHVIKDAAPCPGVEHVGGDMFE 837
           YKGFEG+  +VDVGGG GA + +IVSKYP+IKGINFDLPHVI+DA   PGV+HVGGDMF 
Sbjct: 195 YKGFEGLTSIVDVGGGTGAVVNMIVSKYPSIKGINFDLPHVIEDAPQYPGVQHVGGDMFV 254

Query: 838 NVPKGDVIFMK 870
           +VPKGD IFMK
Sbjct: 255 SVPKGDAIFMK 265



 Score =  104 bits (260), Expect(2) = e-103
 Identities = 47/103 (45%), Positives = 66/103 (64%)
 Frame = +2

Query: 941  WILHDWGDNYCIKLLKNCWAALPEYGKVVVVETIIPDPEHQHSATDVSQNAIGSDIIMLI 1120
            WI HDW D +C+K LKNC+AALP+ GKV++ E I+P       AT   +  +  D+IML 
Sbjct: 266  WICHDWSDEHCLKFLKNCYAALPDNGKVILGECILPVAPDTSLAT---KGVVHIDVIMLA 322

Query: 1121 ANPGGKERMAKEYDTLAKEAGFTSLKIVCRVSTFYIMEFYKNI 1249
             NPGGKER  +E++ LAK +GF  +++ C     Y++EF K I
Sbjct: 323  HNPGGKERTGQEFEALAKGSGFQGIRVACNAFNTYVIEFLKKI 365


>sp|Q8GU25.1|COMT1_ROSCH RecName: Full=Caffeic acid 3-O-methyltransferase; Short=CAOMT;
           Short=COMT; AltName:
           Full=S-adenosysl-L-methionine:caffeic acid
           3-O-methyltransferase gi|27527920|emb|CAD29457.1|
           caffeic acid O-methyltransferase [Rosa chinensis]
          Length = 365

 Score =  298 bits (763), Expect(2) = e-103
 Identities = 151/251 (60%), Positives = 189/251 (75%), Gaps = 1/251 (0%)
 Frame = +1

Query: 121 NEEEGLLRVMQLNTRIVLPMLIKTAIELDLFEIMAKT-PGAQFSSYDLASNLPRQSPQTP 297
           ++EE  L  MQL +  VLPM++K AIELDL EIMAK  PGA  S  DLAS LP ++P+ P
Sbjct: 15  SDEEANLFAMQLASASVLPMVLKAAIELDLLEIMAKAGPGAFLSPNDLASQLPTKNPEAP 74

Query: 298 VLIERILRFLASQSILASTLVTDERGNSKTLYGMTPMSNNFVRNQDGTSLASSLLLIYDK 477
           V+++R+LR LAS SIL  +L T   G  + LYG+ P+     +N+DG S+A+  L+  DK
Sbjct: 75  VMLDRMLRLLASYSILTYSLRTLPDGKVERLYGLGPVCKFLTKNEDGVSIAALCLMNQDK 134

Query: 478 VLVDCWYHLKDAVVEGGIPFDKAHGMNAFEYPAKDNRFNQVFNKSMYDNTKIVMKMILEK 657
           VLV+ WYHLKDAV++GGIPF+KA+GM AF+Y   D RFN+VFNK M D++ I MK ILE 
Sbjct: 135 VLVESWYHLKDAVLDGGIPFNKAYGMTAFDYHGTDPRFNKVFNKGMADHSTITMKKILET 194

Query: 658 YKGFEGVKELVDVGGGLGATLEIIVSKYPNIKGINFDLPHVIKDAAPCPGVEHVGGDMFE 837
           YKGFEG+  +VDVGGG GA + +IVSKYP+IKGINFDLPHVI+DA   PGV+HVGGDMF 
Sbjct: 195 YKGFEGLTSIVDVGGGTGAVVNMIVSKYPSIKGINFDLPHVIEDAPQYPGVQHVGGDMFV 254

Query: 838 NVPKGDVIFMK 870
           +VPKGD IFMK
Sbjct: 255 SVPKGDAIFMK 265



 Score =  103 bits (258), Expect(2) = e-103
 Identities = 46/103 (44%), Positives = 66/103 (64%)
 Frame = +2

Query: 941  WILHDWGDNYCIKLLKNCWAALPEYGKVVVVETIIPDPEHQHSATDVSQNAIGSDIIMLI 1120
            WI HDW D +C+K LKNC+AALP+ GKV++ E I+P       AT   +  +  D++ML 
Sbjct: 266  WICHDWSDEHCLKFLKNCYAALPDNGKVILGECILPVAPDTSLAT---KGVVHIDVVMLA 322

Query: 1121 ANPGGKERMAKEYDTLAKEAGFTSLKIVCRVSTFYIMEFYKNI 1249
             NPGGKER  +E++ LAK +GF  +++ C     Y++EF K I
Sbjct: 323  HNPGGKERTEQEFEALAKGSGFQGIRVACNAFNTYVIEFLKKI 365


>gb|AFK39235.1| unknown [Medicago truncatula]
          Length = 365

 Score =  293 bits (750), Expect(2) = e-102
 Identities = 144/251 (57%), Positives = 188/251 (74%), Gaps = 1/251 (0%)
 Frame = +1

Query: 121 NEEEGLLRVMQLNTRIVLPMLIKTAIELDLFEIMAKT-PGAQFSSYDLASNLPRQSPQTP 297
           ++EE  L  MQL +  VLPM++K+A+ELDL EI+AK  PGAQ S  ++AS LP  +P+ P
Sbjct: 15  SDEEANLFAMQLASASVLPMVLKSALELDLLEIIAKAGPGAQISPIEIASQLPTTNPEAP 74

Query: 298 VLIERILRFLASQSILASTLVTDERGNSKTLYGMTPMSNNFVRNQDGTSLASSLLLIYDK 477
           V+++RILR LA  +IL  ++ T + G  + LYG+  ++   V+N+DG S+++  L+  DK
Sbjct: 75  VMLDRILRLLACYNILTCSVRTQQDGKVQRLYGLATVAKYLVKNEDGVSISALNLMNQDK 134

Query: 478 VLVDCWYHLKDAVVEGGIPFDKAHGMNAFEYPAKDNRFNQVFNKSMYDNTKIVMKMILEK 657
           VL++ WYHLKDAV++GGIPF+KA+GM AFEY   D RFN+VFNK M D++ I MK ILE 
Sbjct: 135 VLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILET 194

Query: 658 YKGFEGVKELVDVGGGLGATLEIIVSKYPNIKGINFDLPHVIKDAAPCPGVEHVGGDMFE 837
           Y GFEG+K LVDVGGG GA +  IVSKYP IKGINFDLPHVI+DA   PGVEHVGGDMF 
Sbjct: 195 YTGFEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFV 254

Query: 838 NVPKGDVIFMK 870
           ++PK D +FMK
Sbjct: 255 SIPKADAVFMK 265



 Score =  108 bits (269), Expect(2) = e-102
 Identities = 51/103 (49%), Positives = 64/103 (62%)
 Frame = +2

Query: 941  WILHDWGDNYCIKLLKNCWAALPEYGKVVVVETIIPDPEHQHSATDVSQNAIGSDIIMLI 1120
            WI HDW D +C+K LKNC+ ALP+ GKV+V E I+P       AT   +  +  D IML 
Sbjct: 266  WICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVAPDSSLAT---KGVVHIDAIMLA 322

Query: 1121 ANPGGKERMAKEYDTLAKEAGFTSLKIVCRVSTFYIMEFYKNI 1249
             NPGGKER  KE++ LAK AGF   K+ C     YIMEF+K +
Sbjct: 323  HNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNTYIMEFFKKV 365