BLASTX nr result
ID: Atractylodes21_contig00011431
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes21_contig00011431
(1250 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBL42917.1| kaurene synthase [Helianthus annuus] gi|29835728... 357 e-162
dbj|BAB12441.1| ent-kaurene synthase No1 [Lactuca sativa] 355 e-160
gb|AAD34295.1|AF097311_1 kaurene synthase [Stevia rebaudiana] 338 e-157
gb|AAD34294.1|AF097310_1 kaurene synthase [Stevia rebaudiana] 329 e-154
emb|CBL42916.1| kaurene synthase [Helianthus annuus] gi|29835728... 333 e-144
>emb|CBL42917.1| kaurene synthase [Helianthus annuus] gi|298357288|emb|CBM82409.1|
kaurene synthase [Helianthus annuus]
Length = 772
Score = 357 bits (916), Expect(2) = e-162
Identities = 188/278 (67%), Positives = 215/278 (77%)
Frame = +2
Query: 2 MVDTLERLGIARHFRVEIQNVLDETYRCWMQRDDQIFMDVVTCALAFRVLRTNGYEVSSD 181
MVDTLERLGIARHF VE++NVLDETYRCW++RD+QIFMDVVTCALAFR+LR NGYEVSSD
Sbjct: 281 MVDTLERLGIARHFSVEVKNVLDETYRCWVERDEQIFMDVVTCALAFRLLRINGYEVSSD 340
Query: 182 PLAEITKEGAYMNALEESFKGVYAALEVXXXXXXXXXXXXXXXXXXXXXXAALEVYRASQ 361
PLAEIT EGA FK YAA+EV Y ASQ
Sbjct: 341 PLAEITNEGA--------FKDEYAAVEV---------------------------YNASQ 365
Query: 362 IIHHKESAFGEHNLRLSNFLKRKISTASNRHSKFIHKEVENALKFPFNASLERIATKRNI 541
+++ +E +FGE LR + L+R IST SNR SKFIHKEVENALKFPFN LERI T+RNI
Sbjct: 366 VLYPEELSFGEQILRSGDILRRMISTDSNRLSKFIHKEVENALKFPFNTGLERINTRRNI 425
Query: 542 EHYNVNETRILKTTYRSSNISNEDYLRLAVEDFNACQSIYRGELRVLERWVVEQNLDKLK 721
+ YNV++TRILKTTYRSSNISN+DYLR AVED+N+CQSIYR EL+ LERWVV+ LD+LK
Sbjct: 426 DRYNVDDTRILKTTYRSSNISNKDYLRFAVEDYNSCQSIYREELKGLERWVVDNKLDQLK 485
Query: 722 FARQKTAYCYFSAASTLSSPELSDARISWAKSSILTTM 835
FARQKTAYCYFS A+TLSSPELSDARIS AK+ ILTT+
Sbjct: 486 FARQKTAYCYFSVAATLSSPELSDARISRAKNGILTTV 523
Score = 244 bits (622), Expect(2) = e-162
Identities = 112/141 (79%), Positives = 127/141 (90%)
Frame = +3
Query: 828 QRWNVNVDNDCCSEEVRIIFLALKDAVCWIGDKAFKWQARDITSHVIEIWLDLMKSMLRE 1007
++WNV+VDNDCCSE+VRI+FLALKDA+CWIGD AFKWQ RD+TSHVI+ WLDLM SMLRE
Sbjct: 545 EKWNVDVDNDCCSEQVRIVFLALKDAICWIGDTAFKWQERDVTSHVIQTWLDLMNSMLRE 604
Query: 1008 AIWLRDECVPTIDEYMENGYISFALGPIVLPALYFVGPRLSEEIVQSSEYHNLFKLLSTR 1187
AIW RD VPTI+EYMEN Y+SFALGPIVLPA+YFVGPRLSEEIV+SSEYH L++L+ST
Sbjct: 605 AIWTRDAYVPTINEYMENAYVSFALGPIVLPAIYFVGPRLSEEIVESSEYHYLYELMSTL 664
Query: 1188 GRLLNDIQGFKREMKAGKLNA 1250
GRLLNDI FKRE K GKLNA
Sbjct: 665 GRLLNDIHSFKREYKEGKLNA 685
>dbj|BAB12441.1| ent-kaurene synthase No1 [Lactuca sativa]
Length = 788
Score = 355 bits (910), Expect(2) = e-160
Identities = 190/278 (68%), Positives = 217/278 (78%)
Frame = +2
Query: 2 MVDTLERLGIARHFRVEIQNVLDETYRCWMQRDDQIFMDVVTCALAFRVLRTNGYEVSSD 181
MVDTLERLGI RHF VEIQNVLDETYRCW+Q D QIFMDVVTCALAFRVLR+NGYEVSSD
Sbjct: 292 MVDTLERLGIKRHFMVEIQNVLDETYRCWVQGDVQIFMDVVTCALAFRVLRSNGYEVSSD 351
Query: 182 PLAEITKEGAYMNALEESFKGVYAALEVXXXXXXXXXXXXXXXXXXXXXXAALEVYRASQ 361
PLA+ITKEG YMN+ E+ FK VY +LEV Y+ASQ
Sbjct: 352 PLAKITKEGDYMNSPEKPFKDVYTSLEV---------------------------YKASQ 384
Query: 362 IIHHKESAFGEHNLRLSNFLKRKISTASNRHSKFIHKEVENALKFPFNASLERIATKRNI 541
II+ +E AF E NL +++L +SN+ S +I KEV++ALKFPFN SLER++T+RNI
Sbjct: 385 IIYQEELAFREQNL--TSYLP-----SSNKLSNYILKEVDDALKFPFNGSLERMSTRRNI 437
Query: 542 EHYNVNETRILKTTYRSSNISNEDYLRLAVEDFNACQSIYRGELRVLERWVVEQNLDKLK 721
EHYN+N TRILKTTY SSNISN+DYL+LAV+DFN CQSIY EL+ LERWVVE LDKLK
Sbjct: 438 EHYNLNHTRILKTTYSSSNISNKDYLKLAVQDFNECQSIYCEELKDLERWVVENRLDKLK 497
Query: 722 FARQKTAYCYFSAASTLSSPELSDARISWAKSSILTTM 835
FARQKTAYCYFSAAS LSSP+LSDARISWAKSSILTT+
Sbjct: 498 FARQKTAYCYFSAASFLSSPDLSDARISWAKSSILTTV 535
Score = 238 bits (607), Expect(2) = e-160
Identities = 109/141 (77%), Positives = 129/141 (91%)
Frame = +3
Query: 828 QRWNVNVDNDCCSEEVRIIFLALKDAVCWIGDKAFKWQARDITSHVIEIWLDLMKSMLRE 1007
++WNVNV+NDCCSEEV ++FLALKDAVCWIGDKAFK Q R+ITSHVIEIWLDL+KSMLRE
Sbjct: 557 EKWNVNVENDCCSEEVGVLFLALKDAVCWIGDKAFKIQERNITSHVIEIWLDLVKSMLRE 616
Query: 1008 AIWLRDECVPTIDEYMENGYISFALGPIVLPALYFVGPRLSEEIVQSSEYHNLFKLLSTR 1187
AIW +D +PTI+EYMENGY+SFALGPIVLP LYF+G +LSEE+VQSSEYH L++++ST+
Sbjct: 617 AIWAKDGSIPTINEYMENGYVSFALGPIVLPTLYFLGVKLSEEVVQSSEYHKLYEVMSTQ 676
Query: 1188 GRLLNDIQGFKREMKAGKLNA 1250
GRL+NDI FKRE KAGKLNA
Sbjct: 677 GRLMNDIHSFKREKKAGKLNA 697
>gb|AAD34295.1|AF097311_1 kaurene synthase [Stevia rebaudiana]
Length = 784
Score = 338 bits (867), Expect(2) = e-157
Identities = 180/278 (64%), Positives = 207/278 (74%)
Frame = +2
Query: 2 MVDTLERLGIARHFRVEIQNVLDETYRCWMQRDDQIFMDVVTCALAFRVLRTNGYEVSSD 181
MVDT+ERLGI+ HFRVEI+NVLDETYRCW++RD+QIFMDVVTCALAFR+LR NGYEVS D
Sbjct: 284 MVDTIERLGISHHFRVEIKNVLDETYRCWVERDEQIFMDVVTCALAFRLLRINGYEVSPD 343
Query: 182 PLAEITKEGAYMNALEESFKGVYAALEVXXXXXXXXXXXXXXXXXXXXXXAALEVYRASQ 361
PLAEIT E AL++ + AALE Y AS
Sbjct: 344 PLAEITNE----LALKDEY-------------------------------AALETYHASH 368
Query: 362 IIHHKESAFGEHNLRLSNFLKRKISTASNRHSKFIHKEVENALKFPFNASLERIATKRNI 541
I++ ++ + G+ L+ ++FLK IST SNR SK IHKEVENALKFP N LERI T+RNI
Sbjct: 369 ILYQEDLSSGKQILKSADFLKEIISTDSNRLSKLIHKEVENALKFPINTGLERINTRRNI 428
Query: 542 EHYNVNETRILKTTYRSSNISNEDYLRLAVEDFNACQSIYRGELRVLERWVVEQNLDKLK 721
+ YNV+ TRILKTTY SSNISN DYLRLAVEDF CQSIYR EL+ LERWVVE LD+LK
Sbjct: 429 QLYNVDNTRILKTTYHSSNISNTDYLRLAVEDFYTCQSIYREELKGLERWVVENKLDQLK 488
Query: 722 FARQKTAYCYFSAASTLSSPELSDARISWAKSSILTTM 835
FARQKTAYCYFS A+TLSSPELSDARISWAK+ ILTT+
Sbjct: 489 FARQKTAYCYFSVAATLSSPELSDARISWAKNGILTTV 526
Score = 244 bits (624), Expect(2) = e-157
Identities = 111/141 (78%), Positives = 128/141 (90%)
Frame = +3
Query: 828 QRWNVNVDNDCCSEEVRIIFLALKDAVCWIGDKAFKWQARDITSHVIEIWLDLMKSMLRE 1007
++WNV+VD DCCSE VRI+FLALKDA+CWIGD+AFKWQARD+TSHVI+ WL+LM SMLRE
Sbjct: 548 EKWNVDVDKDCCSEHVRILFLALKDAICWIGDEAFKWQARDVTSHVIQTWLELMNSMLRE 607
Query: 1008 AIWLRDECVPTIDEYMENGYISFALGPIVLPALYFVGPRLSEEIVQSSEYHNLFKLLSTR 1187
AIW RD VPT++EYMEN Y+SFALGPIV PA+YFVGP+LSEEIV+SSEYHNLFKL+ST+
Sbjct: 608 AIWTRDAYVPTLNEYMENAYVSFALGPIVKPAIYFVGPKLSEEIVESSEYHNLFKLMSTQ 667
Query: 1188 GRLLNDIQGFKREMKAGKLNA 1250
GRLLNDI FKRE K GKLNA
Sbjct: 668 GRLLNDIHSFKREFKEGKLNA 688
>gb|AAD34294.1|AF097310_1 kaurene synthase [Stevia rebaudiana]
Length = 784
Score = 329 bits (843), Expect(2) = e-154
Identities = 177/278 (63%), Positives = 205/278 (73%)
Frame = +2
Query: 2 MVDTLERLGIARHFRVEIQNVLDETYRCWMQRDDQIFMDVVTCALAFRVLRTNGYEVSSD 181
MVDT+ERLGI+ HFRVEI+NVLDETYRCW++RD+QIFMDVVTCALAFR+LR +GY+VS D
Sbjct: 284 MVDTIERLGISHHFRVEIKNVLDETYRCWVERDEQIFMDVVTCALAFRLLRIHGYKVSPD 343
Query: 182 PLAEITKEGAYMNALEESFKGVYAALEVXXXXXXXXXXXXXXXXXXXXXXAALEVYRASQ 361
LAEIT E A FK Y AALE Y ASQ
Sbjct: 344 QLAEITNELA--------FKDEY---------------------------AALETYHASQ 368
Query: 362 IIHHKESAFGEHNLRLSNFLKRKISTASNRHSKFIHKEVENALKFPFNASLERIATKRNI 541
I++ ++ + G+ L+ ++FLK +ST SNR SK IHKEVENALKFP N LERI T+RNI
Sbjct: 369 ILYQEDLSSGKQILKSADFLKGILSTDSNRLSKLIHKEVENALKFPINTGLERINTRRNI 428
Query: 542 EHYNVNETRILKTTYRSSNISNEDYLRLAVEDFNACQSIYRGELRVLERWVVEQNLDKLK 721
+ YNV+ TRILKTTY SSNISN YLRLAVEDF CQSIYR EL+ LERWVV+ LD+LK
Sbjct: 429 QLYNVDNTRILKTTYHSSNISNTYYLRLAVEDFYTCQSIYREELKGLERWVVQNKLDQLK 488
Query: 722 FARQKTAYCYFSAASTLSSPELSDARISWAKSSILTTM 835
FARQKTAYCYFS A+TLSSPELSDARISWAK+ ILTT+
Sbjct: 489 FARQKTAYCYFSVAATLSSPELSDARISWAKNGILTTV 526
Score = 244 bits (624), Expect(2) = e-154
Identities = 111/141 (78%), Positives = 128/141 (90%)
Frame = +3
Query: 828 QRWNVNVDNDCCSEEVRIIFLALKDAVCWIGDKAFKWQARDITSHVIEIWLDLMKSMLRE 1007
++WNV+VD DCCSE VRI+FLALKDA+CWIGD+AFKWQARD+TSHVI+ WL+LM SMLRE
Sbjct: 548 EKWNVDVDKDCCSEHVRILFLALKDAICWIGDEAFKWQARDVTSHVIQTWLELMNSMLRE 607
Query: 1008 AIWLRDECVPTIDEYMENGYISFALGPIVLPALYFVGPRLSEEIVQSSEYHNLFKLLSTR 1187
AIW RD VPT++EYMEN Y+SFALGPIV PA+YFVGP+LSEEIV+SSEYHNLFKL+ST+
Sbjct: 608 AIWTRDAYVPTLNEYMENAYVSFALGPIVKPAIYFVGPKLSEEIVESSEYHNLFKLMSTQ 667
Query: 1188 GRLLNDIQGFKREMKAGKLNA 1250
GRLLNDI FKRE K GKLNA
Sbjct: 668 GRLLNDIHSFKREFKEGKLNA 688
>emb|CBL42916.1| kaurene synthase [Helianthus annuus] gi|298357286|emb|CBM82408.1|
kaurene synthase [Helianthus annuus]
Length = 774
Score = 333 bits (854), Expect(2) = e-144
Identities = 176/278 (63%), Positives = 204/278 (73%)
Frame = +2
Query: 2 MVDTLERLGIARHFRVEIQNVLDETYRCWMQRDDQIFMDVVTCALAFRVLRTNGYEVSSD 181
MVDTLERLGIARHF VEI+NVLDETYRCW++RD+QIFMDVVTCALAFR+LR NGYEVS D
Sbjct: 284 MVDTLERLGIARHFSVEIKNVLDETYRCWVERDEQIFMDVVTCALAFRLLRINGYEVSPD 343
Query: 182 PLAEITKEGAYMNALEESFKGVYAALEVXXXXXXXXXXXXXXXXXXXXXXAALEVYRASQ 361
PL EIT EGA FK YAALEV Y ASQ
Sbjct: 344 PLEEITNEGA--------FKDEYAALEV---------------------------YNASQ 368
Query: 362 IIHHKESAFGEHNLRLSNFLKRKISTASNRHSKFIHKEVENALKFPFNASLERIATKRNI 541
I++ KE AFGE LR +FL+R ST SNR S FI KE ENALK PFN +ERI KR I
Sbjct: 369 ILYPKELAFGEQILRSGDFLRRITSTDSNRLSTFIQKEAENALKVPFNTGIERINVKRYI 428
Query: 542 EHYNVNETRILKTTYRSSNISNEDYLRLAVEDFNACQSIYRGELRVLERWVVEQNLDKLK 721
+HY+V+ TRILKTTYRSSNISNEDYL+ AVEDFN+CQSIY E++ +RW+ E LD+L
Sbjct: 429 DHYHVDHTRILKTTYRSSNISNEDYLKFAVEDFNSCQSIYLEEMKGFKRWLAETKLDQLT 488
Query: 722 FARQKTAYCYFSAASTLSSPELSDARISWAKSSILTTM 835
FAR+K+AYCYF+AA+TL +PELSDAR+SWAK+SILT +
Sbjct: 489 FAREKSAYCYFTAAATLPAPELSDARLSWAKNSILTAI 526
Score = 207 bits (528), Expect(2) = e-144
Identities = 96/141 (68%), Positives = 115/141 (81%)
Frame = +3
Query: 828 QRWNVNVDNDCCSEEVRIIFLALKDAVCWIGDKAFKWQARDITSHVIEIWLDLMKSMLRE 1007
++WNV+VD DCCSE VRI+F A+KDA+CW+GD FKWQ RD+TSHV +IWLDL+ SML E
Sbjct: 548 EKWNVDVDKDCCSETVRILFSAIKDAICWMGDAGFKWQERDVTSHVTQIWLDLLNSMLTE 607
Query: 1008 AIWLRDECVPTIDEYMENGYISFALGPIVLPALYFVGPRLSEEIVQSSEYHNLFKLLSTR 1187
AIW RD PT+DEYM+N +SFALGPIVL LYFVGP+LSEEIV+SSEYHN+F +S
Sbjct: 608 AIWRRDAYTPTMDEYMKNASVSFALGPIVLNTLYFVGPKLSEEIVESSEYHNIFDQMSVM 667
Query: 1188 GRLLNDIQGFKREMKAGKLNA 1250
GRLLNDI F RE+K +LNA
Sbjct: 668 GRLLNDIYSFMREIKE-ELNA 687