BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00011082
         (3089 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]              1056   0.0  
dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]      1039   0.0  
dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]               1024   0.0  
dbj|BAK32962.1| topoisomerase I [Ophiorrhiza trichocarpon var. g...  1012   0.0  
dbj|BAK32958.1| topoisomerase I [Ophiorrhiza pedunculata]            1008   0.0  

>dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]
          Length = 894

 Score = 1056 bits (2731), Expect = 0.0
 Identities = 562/907 (61%), Positives = 660/907 (72%), Gaps = 8/907 (0%)
 Frame = -3

Query: 2997 EDDKPLSSRISTVSKANSNHSKIGPNTSASQRPKPHAEDDKPLSSRISTVPKANSNHSKI 2818
            EDD+PL   +   S A S H+++  +T  S +       D     ++S VP  N      
Sbjct: 17   EDDEPL---VFKRSNATSKHNQLNSDTKKSSQ-----RYDGQSGRQVSDVPSTN------ 62

Query: 2817 GPSTSASQRPKPQAEDDKPLSSRISPV--PKANSNHSKIGPNTSASQRPKPQAEDEKPLS 2644
            G S+SAS+    +      LSS  SP+  PKA+++ +K  P   A+ +    ++D    S
Sbjct: 63   GQSSSASKGKTTK------LSSMKSPIASPKASTSFAKASP--VANPKVSSSSDDRSKHS 114

Query: 2643 SRISPVPKANSNNSKIGLSTSASQRPKPQAEDDKSLSSRI-SPVPKANSNHSK---IGPS 2476
            S+ + +         +    + +  P   +ED+K LS+R+ + + K +SN++    I  S
Sbjct: 115  SKQNTINVVKEEKELV----NPAAEPYGDSEDEKPLSARLFTGLTKGSSNNANKGLINSS 170

Query: 2475 MSASQCPKPQVK--NEDSDDEKPLSSKFTSKSSVGASIGKRDDSDDDKPLSLRVKQNGSA 2302
             ++   PKP++   ++DSDDE PLSSKF  K++ G S  K  +SDD+K L    +QNGS 
Sbjct: 171  PASLPVPKPEINRYSDDSDDEIPLSSKFRLKANAGTSTVKSYESDDNKTLVSNFQQNGSI 230

Query: 2301 SRDNQSIKPSIGLNKRPPNEERSSGPSSVKKPKLSDSSTPINRKPVSVKPEAETDDDEND 2122
            +R ++S   SI +NKRP  E +SS  SSVKKPKLSD+STP+N K  S K E + DD ++D
Sbjct: 231  NRGSKS---SIKVNKRPLGEVKSSVQSSVKKPKLSDASTPVNNKQASKKAEPKADDSDDD 287

Query: 2121 HVPISQRMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 1942
            HVPISQRM                                                    
Sbjct: 288  HVPISQRMKKSATSGNKSSTAKQKVTKVVSSSMKKASKKFKKVVKKSKYSKSSKVPPSSG 347

Query: 1941 XGQKWTSLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKPVF 1762
             G+KW +LVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTK  F
Sbjct: 348  EGKKWNTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKSKF 407

Query: 1761 KENFMNDWRKILGRNHTIQNLDDCDFRPIYEWHQREKEKKKQMSTXXXXXXXXXXXXXXX 1582
            KENFM+DWRKILG+NH IQNL+DCDF PIYEWHQREKEKKK+M+                
Sbjct: 408  KENFMDDWRKILGKNHVIQNLEDCDFTPIYEWHQREKEKKKKMTAEEKKVLKEERLKQEE 467

Query: 1581 KYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKTRIRPSDITINIGKGAPIPECP 1402
            KYMWAIV+GVKEKVGNFRVEPP LFRGRGEHPKMGKLK  IRPSDITINIGK APIPECP
Sbjct: 468  KYMWAIVNGVKEKVGNFRVEPPSLFRGRGEHPKMGKLKKCIRPSDITINIGKDAPIPECP 527

Query: 1401 IPGQSWKEVRHDNTVTWLAYWNDPINPKDFKYVFLAASSALKGQSDKEKYEKARLLKGYI 1222
            IPG+SWKE+RHDNTVTWLA+WNDPI P++FKYVFLAASS+LKGQSDKEKYEKARLLK +I
Sbjct: 528  IPGESWKEIRHDNTVTWLAFWNDPIKPREFKYVFLAASSSLKGQSDKEKYEKARLLKDFI 587

Query: 1221 EGIRKAYTRDFTNKDVTKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPK 1042
            +GIR AYT+DF +KD+TKRQ+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPK
Sbjct: 588  QGIRAAYTKDFASKDITKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPK 647

Query: 1041 PPNILKFDFLGKDSIRYQNEVEVELPVFKAIQQFRTXXXXXXXXXXXXDTSTLNAHLKEL 862
            PP+ILKFDFLGKDSIRYQNEVEVELPV+KAIQQFRT            DTS LNAHLK L
Sbjct: 648  PPSILKFDFLGKDSIRYQNEVEVELPVYKAIQQFRTGKRGGDDLFDKLDTSKLNAHLKGL 707

Query: 861  MPGLTAKVFRTYNASITLDDMLNRDMKGGNLAENVVVYNQANKEVAIICNHQRTVSKSHS 682
            MPGLTAKVFRTYNASITLD+ML+R+ KGGN+AE +VVY  ANKEVAIICNHQRTVSKSH 
Sbjct: 708  MPGLTAKVFRTYNASITLDEMLSRETKGGNVAEKIVVYQHANKEVAIICNHQRTVSKSHP 767

Query: 681  AQMMRLNDKIEELKGVVEELETDLARAKKGKPPLKGSDGKSKRNMNPEALQKKIDQTNAK 502
            AQM RLN KI+ELKG+++ L+TDLARAKKGKPPL+ +DGK KRN+ PEAL++KI QTNAK
Sbjct: 768  AQMTRLNGKIDELKGILDGLQTDLARAKKGKPPLEDADGKPKRNLTPEALERKIGQTNAK 827

Query: 501  IEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRRDVPIEKIFNKSLLAKFAWSMD 322
            IEKMERDKETKE LKT+ALGTSKI+YLDPRITVAWCKR +VPIEK+FNKSLLAKFAWSMD
Sbjct: 828  IEKMERDKETKEGLKTIALGTSKISYLDPRITVAWCKRHEVPIEKVFNKSLLAKFAWSMD 887

Query: 321  VDPSFRF 301
            VDPSFRF
Sbjct: 888  VDPSFRF 894


>dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]
          Length = 925

 Score = 1039 bits (2686), Expect = 0.0
 Identities = 573/948 (60%), Positives = 643/948 (67%), Gaps = 25/948 (2%)
 Frame = -3

Query: 3069 SKANSNHSKIGPNTSASQRPKPHAEDDKPLSSRISTVSKANSNHSKIGPNTSASQRPKPH 2890
            ++ANS   K+       Q    H  D +P +   S+  K     S   P   +       
Sbjct: 33   NQANSEKKKLSLQKQDGQSAV-HTSDVRPANGESSSAQKGRIQPSAKTPPVKS------- 84

Query: 2889 AEDDKPLSSRISTVPKANSNHSKIGPSTSASQRPKPQAEDDKPLSSRISPVPKANSNHSK 2710
                 PLSS     PKA+++ +K  P TS     KP A        +         NH K
Sbjct: 85   -----PLSS-----PKASNSSAKASPITSPGANSKPSASATSTAKQQ---------NHHK 125

Query: 2709 IGPNTSASQRPKPQAEDEKPLSSRISPVPKANSNNSKIGLSTSASQRPKPQAEDDKSLSS 2530
               +++A   PKP             P  KANSN+                ++D+K LS+
Sbjct: 126  ---SSAAFIEPKPSV-----------PAAKANSNSES--------------SDDEKPLSA 157

Query: 2529 R-ISPVPKANSNHSKIGPSMSASQCPKPQVKNEDSDDEKPLSSKFTSKSSVGASIGKRDD 2353
            R      K  SNH+      S S       ++EDS+DEKPLS +FTSKSS+G S  K  D
Sbjct: 158  RTFGCSSKGKSNHANKEGGDSCSIRRPVIEESEDSEDEKPLSCRFTSKSSLGESTSKSYD 217

Query: 2352 SDDDKPLSLRVKQNGSASRDNQSIKPSIGLNKRPPNEERSSGPSSVKKPKLSDSSTPINR 2173
            SD+ KPL+ +V+QNGSASRD    K +   NKRPP E +S   SSVKKPKLS   T I+ 
Sbjct: 218  SDEKKPLAAKVQQNGSASRDGPMNKSASLSNKRPPGEAKSLNQSSVKKPKLSSPITSISN 277

Query: 2172 KPVSVKPE--AETDDD---------------------ENDHVPISQRMXXXXXXXXXXXX 2062
            K  +VKPE  AE DDD                     ++D+VPISQR+            
Sbjct: 278  KQAAVKPEPKAEDDDDNIPLSQRGKKSQSATSKPKDDDDDNVPISQRIKKSPASVSKSSV 337

Query: 2061 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGQKWTSLVHNGVIFPPPYK 1882
                                                     GQKWT+LVHNGVIFPPPYK
Sbjct: 338  PVKKASTVVSSSLKKVNKNSKKVIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYK 397

Query: 1881 PHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKPVFKENFMNDWRKILGRNHTIQN 1702
            PHGVKMLYK +P+ LTPEQEEVATMFAVMLDTDYM KP FKENF +DW+KILG+NH IQN
Sbjct: 398  PHGVKMLYKRQPITLTPEQEEVATMFAVMLDTDYMNKPCFKENFFSDWKKILGKNHMIQN 457

Query: 1701 LDDCDFRPIYEWHQREKEKKKQMSTXXXXXXXXXXXXXXXKYMWAIVDGVKEKVGNFRVE 1522
            L+DCDF PIYEWHQ+EKEKKKQM+T               KYMWAIVDGVKEKVGNFRVE
Sbjct: 458  LEDCDFGPIYEWHQQEKEKKKQMTTEEKKALKEEKLQQEEKYMWAIVDGVKEKVGNFRVE 517

Query: 1521 PPGLFRGRGEHPKMGKLKTRIRPSDITINIGKGAPIPECPIPGQSWKEVRHDNTVTWLAY 1342
            PPGLFRGRGEHPKMGKLK RIRP DITINIGK APIPECPIPG+ WKEVR+ NTVTWLAY
Sbjct: 518  PPGLFRGRGEHPKMGKLKKRIRPRDITINIGKDAPIPECPIPGERWKEVRNGNTVTWLAY 577

Query: 1341 WNDPINPKDFKYVFLAASSALKGQSDKEKYEKARLLKGYIEGIRKAYTRDFTN-KDVTKR 1165
            WNDPIN K+FKYVFLAASS LKGQSDKEKYEKARLLK YI GIR AYT+DFTN KD  K+
Sbjct: 578  WNDPINQKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKK 637

Query: 1164 QVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPKPPNILKFDFLGKDSIRYQN 985
            Q+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEP PPNILKFDFLGKDSIRYQN
Sbjct: 638  QIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQN 697

Query: 984  EVEVELPVFKAIQQFRTXXXXXXXXXXXXDTSTLNAHLKELMPGLTAKVFRTYNASITLD 805
            EV+VE  VFKAIQQFR+            DTS LNAHLKELMPGLTAKVFRTYNASITLD
Sbjct: 698  EVQVEPAVFKAIQQFRSGKEGSEDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLD 757

Query: 804  DMLNRDMKGGNLAENVVVYNQANKEVAIICNHQRTVSKSHSAQMMRLNDKIEELKGVVEE 625
            DML+++ KGG +AENVVVY  ANKEVAIICNHQRTVSKSHSAQM RLN+KI+ELK  +EE
Sbjct: 758  DMLSKETKGGEVAENVVVYQHANKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTALEE 817

Query: 624  LETDLARAKKGKPPLKGSDGKSKRNMNPEALQKKIDQTNAKIEKMERDKETKEDLKTVAL 445
            L+TDL+RAKKGKPP KG+DGK+KRN+NPEAL++KI QTNAKIEKMERDKETKEDLKTVAL
Sbjct: 818  LKTDLSRAKKGKPPTKGADGKAKRNLNPEALERKITQTNAKIEKMERDKETKEDLKTVAL 877

Query: 444  GTSKINYLDPRITVAWCKRRDVPIEKIFNKSLLAKFAWSMDVDPSFRF 301
            GTSKINYLDPRITVAWCKR +VPIEKIFNKSLLAKFAWSMDVDPSFRF
Sbjct: 878  GTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWSMDVDPSFRF 925


>dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]
          Length = 927

 Score = 1024 bits (2648), Expect = 0.0
 Identities = 566/953 (59%), Positives = 639/953 (67%), Gaps = 27/953 (2%)
 Frame = -3

Query: 3078 STVSKANSNHSKIGPNTSASQRPKPHAEDDKPLSSRISTVSKANSNHSKIGPNTSASQRP 2899
            S  ++ANS   K+       Q    H  D +P +   S+  K     S   P   +    
Sbjct: 30   SKQNQANSEKKKLSLQKHVGQSAV-HTSDVRPANGESSSAQKGRIQPSAKTPPVKS---- 84

Query: 2898 KPHAEDDKPLSSRISTVPKANSNHSKIGPSTSASQRPKPQAEDDKPLSSRISPVPKANSN 2719
                    PLSS     PKA+++ +K  P TS     KP A        +         N
Sbjct: 85   --------PLSS-----PKASNSSAKASPITSPGANSKPSASATSTAKQQ---------N 122

Query: 2718 HSKIGPNTSASQRPKPQAEDEKPLSSRISPVPKANSNNSKIGLSTSASQRPKPQAEDDKS 2539
            H K   +++A   PKP             P  KANSN+                ++D+K 
Sbjct: 123  HHK---SSAAFIEPKPSV-----------PAAKANSNSES--------------SDDEKP 154

Query: 2538 LSSR-ISPVPKANSNHSKIGPSMSASQCPKPQVKNEDSDDEKPLSSKFTSKSSVGASIGK 2362
            LS+R      K  SNH+      S S   +   ++EDS+DEKPLS +FTSKSS+G S  K
Sbjct: 155  LSARTFGCSSKGKSNHANKEGGDSCSIRRRVIEESEDSEDEKPLSRRFTSKSSLGESTSK 214

Query: 2361 RDDSDDDKPLSLRVKQNGSASRDNQSIKPSIGLNKRPPNEERSSGPSSVKKPKLSDSSTP 2182
              DSD+ KPL+ +V+QNGS SRD    K +   NKRPP E +S   SSVKKPKLS   T 
Sbjct: 215  SYDSDEKKPLAAKVQQNGSVSRDGPMNKSASLSNKRPPGEVKSLNQSSVKKPKLSSPITS 274

Query: 2181 INRKPVSVKPEAET---DDDEN----------------------DHVPISQRMXXXXXXX 2077
            I+ K  +VKPE E    DDD+N                      D+VPI+QR+       
Sbjct: 275  ISNKQAAVKPEPEPKAEDDDDNIPLSQRGKKSQAATSKPKDEDDDNVPIAQRIKKSPASV 334

Query: 2076 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGQKWTSLVHNGVIF 1897
                                                          GQKWT+LVHNGVIF
Sbjct: 335  SKSSAPVKKASTVVSPSLKKVNKNSKKVIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIF 394

Query: 1896 PPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKPVFKENFMNDWRKILGRN 1717
            PPPY PHGVKMLYK +P+ LTPEQEEVATMFAVMLDTDYM KP FKENF +DW+K+LG+N
Sbjct: 395  PPPYNPHGVKMLYKRQPITLTPEQEEVATMFAVMLDTDYMNKPRFKENFFSDWKKMLGKN 454

Query: 1716 HTIQNLDDCDFRPIYEWHQREKEKKKQMSTXXXXXXXXXXXXXXXKYMWAIVDGVKEKVG 1537
            HTIQNL+DCDF PIYEWHQ+EKEKKKQM+T               KYMWAIVDGVKEKVG
Sbjct: 455  HTIQNLEDCDFGPIYEWHQQEKEKKKQMTTEEKKALKEDKLQQEEKYMWAIVDGVKEKVG 514

Query: 1536 NFRVEPPGLFRGRGEHPKMGKLKTRIRPSDITINIGKGAPIPECPIPGQSWKEVRHDNTV 1357
            NFRVEPPGLFRGRGEHPKMGKLK RIRP DITINIGK APIPECPIPG+ WKEVR+DNTV
Sbjct: 515  NFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKDAPIPECPIPGERWKEVRNDNTV 574

Query: 1356 TWLAYWNDPINPKDFKYVFLAASSALKGQSDKEKYEKARLLKGYIEGIRKAYTRDFTN-K 1180
            TWLA+W DPIN K+FKYVFLAASS LKGQSDKEKYEKARLLK YI GIR AYT+DFTN K
Sbjct: 575  TWLAFWIDPINQKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNK 634

Query: 1179 DVTKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPKPPNILKFDFLGKDS 1000
            D  K+Q+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEP PPNILKFDFLGKDS
Sbjct: 635  DPMKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDFLGKDS 694

Query: 999  IRYQNEVEVELPVFKAIQQFRTXXXXXXXXXXXXDTSTLNAHLKELMPGLTAKVFRTYNA 820
            IRYQNEV+VE  VFKAIQQFR+            DTS LNAHLKELMPGLTAKVFRTYNA
Sbjct: 695  IRYQNEVQVEPAVFKAIQQFRSGKEGSEDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNA 754

Query: 819  SITLDDMLNRDMKGGNLAENVVVYNQANKEVAIICNHQRTVSKSHSAQMMRLNDKIEELK 640
            SITLDDML+++ KGG +AE VVVY  ANKEVAIICNHQRTVSKSHSAQM RLN+KI+ELK
Sbjct: 755  SITLDDMLSKETKGGEVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMSRLNEKIDELK 814

Query: 639  GVVEELETDLARAKKGKPPLKGSDGKSKRNMNPEALQKKIDQTNAKIEKMERDKETKEDL 460
              +EEL+TDL+R KKGKPP KG+DGK KRN+NPEAL++K+ Q NAKIEKMERDKETKEDL
Sbjct: 815  TAMEELKTDLSRVKKGKPPSKGADGKPKRNLNPEALERKMAQINAKIEKMERDKETKEDL 874

Query: 459  KTVALGTSKINYLDPRITVAWCKRRDVPIEKIFNKSLLAKFAWSMDVDPSFRF 301
            KTVALGTSKINYLDPRITVAWCKR +VPIEKIFNKSLLAKFAWSMDVDPSFRF
Sbjct: 875  KTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWSMDVDPSFRF 927


>dbj|BAK32962.1| topoisomerase I [Ophiorrhiza trichocarpon var. glabra]
          Length = 920

 Score = 1012 bits (2617), Expect = 0.0
 Identities = 555/895 (62%), Positives = 628/895 (70%), Gaps = 26/895 (2%)
 Frame = -3

Query: 2928 GPNTSASQ-RPKPHAEDDKPLSSRISTVPKANSNHSKIGPSTSASQRPKPQAEDDKPLSS 2752
            G N+SA + R +P A+   P+ S +S+ PKA+++ +K  P TSA    KP A        
Sbjct: 64   GENSSAQKGRIQPSAKTP-PVKSPLSS-PKASNSSAKASPITSAGANSKPSASATSTAKQ 121

Query: 2751 RISPVPKANSNHSKIGPNTSASQRPKPQAEDEKPLSSRISPVPKANSNNSKIGLSTSASQ 2572
            +         NH K   +++A   PKP             P  KANSN+           
Sbjct: 122  Q---------NHHK---SSAAFIEPKPSV-----------PAAKANSNSES--------- 149

Query: 2571 RPKPQAEDDKSLSSR-ISPVPKANSNHSKIGPSMSASQCPKPQVKNEDSDDEKPLSSKFT 2395
                 ++D+K LS+R      K  SNH+      S S       ++EDS+DEKPLS +FT
Sbjct: 150  -----SDDEKPLSARTFGCSSKGKSNHANKEGGDSCSIRRPVIEESEDSEDEKPLSRRFT 204

Query: 2394 SKSSVGASIGKRDDSDDDKPLSLRVKQNGSASRDNQSIKPSIGLNKRPPNEERSSGPSSV 2215
            SKSS+G S  K  DSD+ KPL+ +V+ NGSASRD+   K +   NKRPP E +S   SS+
Sbjct: 205  SKSSLGESTSKSYDSDEKKPLAAKVQPNGSASRDSPMNKSASLSNKRPPGEVKSLNQSSL 264

Query: 2214 KKPKLSDSSTPINRKPVSVKPE--AETDDD---------------------ENDHVPISQ 2104
            KKPKLS   T I+ K  +VKPE  AE DDD                     ++D+VPISQ
Sbjct: 265  KKPKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQRGKKSQTATSKPKDEDDDNVPISQ 324

Query: 2103 RMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGQKWT 1924
            R+                                                     GQKWT
Sbjct: 325  RIKKSPASVSKSSAPVKKASTVVSPSLKKVNKKSKKVIKKTAYTKSSKVPPGSGEGQKWT 384

Query: 1923 SLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKPVFKENFMN 1744
            +LVHNGVIFPPPYKPHGVKMLYK +P+ LTPEQEEVATMFAVMLDTDYM KP FKENF +
Sbjct: 385  TLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEVATMFAVMLDTDYMNKPRFKENFFS 444

Query: 1743 DWRKILGRNHTIQNLDDCDFRPIYEWHQREKEKKKQMSTXXXXXXXXXXXXXXXKYMWAI 1564
            DW+K+LG+NHTIQNL+DCDF PIYEWHQ+EKEKKKQM+T               KYMWAI
Sbjct: 445  DWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKKKQMTTEEKKALKEEKLQQEEKYMWAI 504

Query: 1563 VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKTRIRPSDITINIGKGAPIPECPIPGQSW 1384
            VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLK RIRP DITINIGK APIPECPIPG+ W
Sbjct: 505  VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKDAPIPECPIPGERW 564

Query: 1383 KEVRHDNTVTWLAYWNDPINPKDFKYVFLAASSALKGQSDKEKYEKARLLKGYIEGIRKA 1204
            KEVR+DNTVTWLAYWNDPIN K+FKYVFLAASS LKGQSDKEKYEKARLLK YI GIR A
Sbjct: 565  KEVRNDNTVTWLAYWNDPINQKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIHGIRAA 624

Query: 1203 YTRDFTN-KDVTKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPKPPNIL 1027
            YT+DFTN KD  K+Q+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEP PPNIL
Sbjct: 625  YTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNIL 684

Query: 1026 KFDFLGKDSIRYQNEVEVELPVFKAIQQFRTXXXXXXXXXXXXDTSTLNAHLKELMPGLT 847
            KFDFLGKDSIRYQNEV+VE  VFKAIQQFR+            DTS LNAHLKELMPGLT
Sbjct: 685  KFDFLGKDSIRYQNEVQVEPAVFKAIQQFRSGKEGSEDLFDRLDTSKLNAHLKELMPGLT 744

Query: 846  AKVFRTYNASITLDDMLNRDMKGGNLAENVVVYNQANKEVAIICNHQRTVSKSHSAQMMR 667
            AKVFRTYNASITLDDML++   GG +AE VVVY  ANKEVAIICNHQRTVSKSHSAQM R
Sbjct: 745  AKVFRTYNASITLDDMLSKKTNGGEVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMSR 804

Query: 666  LNDKIEELKGVVEELETDLARAKKGKPPLKGSDGKSKRNMNPEALQKKIDQTNAKIEKME 487
            LN+KI+ELK  +EEL+TDL+RAKKGKP  KG+DGK KRN+NPEAL++KI QTNAKIEKME
Sbjct: 805  LNEKIDELKTALEELKTDLSRAKKGKPLSKGADGKPKRNLNPEALERKIAQTNAKIEKME 864

Query: 486  RDKETKEDLKTVALGTSKINYLDPRITVAWCKRRDVPIEKIFNKSLLAKFAWSMD 322
            RDKETKEDLKTVALGTSKINYLDPRITVAWCKR +VPIEKIFNKSLLAKFAW+M+
Sbjct: 865  RDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMN 919



 Score = 94.0 bits (232), Expect = 2e-16
 Identities = 90/312 (28%), Positives = 147/312 (47%), Gaps = 19/312 (6%)
 Frame = -3

Query: 3066 KANSNHSKIGPNTSASQRPKPHAEDDKPLSSRISTVSKANSNHSKIGPNTSASQRPKPH- 2890
            KA+++ +K  P TSA    KP A          +T +    NH K   +++A   PKP  
Sbjct: 91   KASNSSAKASPITSAGANSKPSAS---------ATSTAKQQNHHK---SSAAFIEPKPSV 138

Query: 2889 -----------AEDDKPLSSR-ISTVPKANSNHS-KIGPSTSASQRP----KPQAEDDKP 2761
                       ++D+KPLS+R      K  SNH+ K G  + + +RP       +ED+KP
Sbjct: 139  PAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDSCSIRRPVIEESEDSEDEKP 198

Query: 2760 LSSRISPVPKANSNHSKIGPNTSASQRPKPQAEDEKPLSSRISPVPKANSNNSKIGLSTS 2581
            LS R +       + S +G +TS S      ++++KPL++++ P   A S +S +  S S
Sbjct: 199  LSRRFT-------SKSSLGESTSKSY----DSDEKKPLAAKVQPNGSA-SRDSPMNKSAS 246

Query: 2580 ASQRPKPQAEDDKSLSSRISPVPKANSNHSKIGPSMSASQCPKPQVKNEDSDDEKPLSSK 2401
             S +  P   + KSL+      PK +S  + I    +A    KP+ K ED DD  PLS +
Sbjct: 247  LSNKRPPG--EVKSLNQSSLKKPKLSSPITSISNKQAAV---KPEPKAEDDDDNIPLSQR 301

Query: 2400 FTSKSSVGASIGKRDDSDDDKPLSLRVKQN-GSASRDNQSIKPSIGLNKRPPNEERSSGP 2224
               K S  A+   +D+ DD+ P+S R+K++  S S+ +  +K +  +      +      
Sbjct: 302  --GKKSQTATSKPKDEDDDNVPISQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKKSK 359

Query: 2223 SSVKKPKLSDSS 2188
              +KK   + SS
Sbjct: 360  KVIKKTAYTKSS 371


>dbj|BAK32958.1| topoisomerase I [Ophiorrhiza pedunculata]
          Length = 920

 Score = 1008 bits (2607), Expect = 0.0
 Identities = 553/895 (61%), Positives = 626/895 (69%), Gaps = 26/895 (2%)
 Frame = -3

Query: 2928 GPNTSASQ-RPKPHAEDDKPLSSRISTVPKANSNHSKIGPSTSASQRPKPQAEDDKPLSS 2752
            G N+SA + R +P A+   P+ S +S+ PKA+++ +K  P TS     KP A        
Sbjct: 64   GENSSAQKGRIQPSAKTP-PVKSPLSS-PKASNSSAKASPITSPGANSKPSASATSTAKQ 121

Query: 2751 RISPVPKANSNHSKIGPNTSASQRPKPQAEDEKPLSSRISPVPKANSNNSKIGLSTSASQ 2572
            +         NH K   +++A   PKP             P  KANSN+           
Sbjct: 122  Q---------NHHK---SSAAFIEPKPSV-----------PAAKANSNSES--------- 149

Query: 2571 RPKPQAEDDKSLSSR-ISPVPKANSNHSKIGPSMSASQCPKPQVKNEDSDDEKPLSSKFT 2395
                 ++D+K LS+R      K  SNH+      S S       ++EDS+DEKPLS +FT
Sbjct: 150  -----SDDEKPLSARTFGCSSKGKSNHANKEGGDSCSIRRPVIEESEDSEDEKPLSRRFT 204

Query: 2394 SKSSVGASIGKRDDSDDDKPLSLRVKQNGSASRDNQSIKPSIGLNKRPPNEERSSGPSSV 2215
            SKSS+G S  K  DSD+ KPL+ +V+ NGSASRD+   K +   NKRPP E +S   SS+
Sbjct: 205  SKSSLGESTSKSYDSDEKKPLAAKVQPNGSASRDSPMNKSASLSNKRPPGEVKSLNQSSL 264

Query: 2214 KKPKLSDSSTPINRKPVSVKPE--AETDDD---------------------ENDHVPISQ 2104
            KKPKLS   T I+ K  +VKPE  AE DDD                     ++D+VPISQ
Sbjct: 265  KKPKLSSPITSISNKQAAVKPEPKAEDDDDNIPLSQRGKKSQTATSKPKDEDDDNVPISQ 324

Query: 2103 RMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGQKWT 1924
            R+                                                     GQKWT
Sbjct: 325  RIKKSPASVSKSSAPVKKASTVVSPSLKKVNKKSKKVIKKTAYTKSSKVPPGSGEGQKWT 384

Query: 1923 SLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKPVFKENFMN 1744
            +LVHNGVIFPPPYKPHGVKMLYK +P+ LTPEQEEVATMFAVMLDTDYM KP FKENF +
Sbjct: 385  TLVHNGVIFPPPYKPHGVKMLYKRQPITLTPEQEEVATMFAVMLDTDYMNKPRFKENFFS 444

Query: 1743 DWRKILGRNHTIQNLDDCDFRPIYEWHQREKEKKKQMSTXXXXXXXXXXXXXXXKYMWAI 1564
            DW+K+LG+NHTIQNL+DCDF PIYEWHQ+EKEKKKQM+T               KYMWAI
Sbjct: 445  DWKKMLGKNHTIQNLEDCDFGPIYEWHQQEKEKKKQMTTEEKKALKEEKLQQEEKYMWAI 504

Query: 1563 VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKTRIRPSDITINIGKGAPIPECPIPGQSW 1384
            VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLK RIRP DITINIGK APIPECPIPG+ W
Sbjct: 505  VDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKDAPIPECPIPGERW 564

Query: 1383 KEVRHDNTVTWLAYWNDPINPKDFKYVFLAASSALKGQSDKEKYEKARLLKGYIEGIRKA 1204
            KEVR+DNTVTWLAYWNDPIN K+FKYVFLAASS LKGQSDKEKYEKARLLK YI GIR A
Sbjct: 565  KEVRNDNTVTWLAYWNDPINQKEFKYVFLAASSTLKGQSDKEKYEKARLLKDYIHGIRAA 624

Query: 1203 YTRDFTN-KDVTKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPKPPNIL 1027
            YT+DFTN KD  K+Q+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEP PPNIL
Sbjct: 625  YTKDFTNNKDPMKKQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPVPPNIL 684

Query: 1026 KFDFLGKDSIRYQNEVEVELPVFKAIQQFRTXXXXXXXXXXXXDTSTLNAHLKELMPGLT 847
            KFDFLGKDSIRYQNEV+VE  VFKAI QFR+            DTS LNAHLKELMPGLT
Sbjct: 685  KFDFLGKDSIRYQNEVQVEPAVFKAIHQFRSGKEGSEDLFDRLDTSKLNAHLKELMPGLT 744

Query: 846  AKVFRTYNASITLDDMLNRDMKGGNLAENVVVYNQANKEVAIICNHQRTVSKSHSAQMMR 667
            AKVFRTYNASITLDDML++   GG +AE VVVY  ANKEVAIICNHQRTVSKSHSAQM R
Sbjct: 745  AKVFRTYNASITLDDMLSKKTNGGEVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMSR 804

Query: 666  LNDKIEELKGVVEELETDLARAKKGKPPLKGSDGKSKRNMNPEALQKKIDQTNAKIEKME 487
            LN+KI+ELK  +EEL+TDL+RAKKGKP  KG+DGK KRN+NPEAL++KI QTNAKIEKME
Sbjct: 805  LNEKIDELKTALEELKTDLSRAKKGKPLSKGADGKPKRNLNPEALERKIAQTNAKIEKME 864

Query: 486  RDKETKEDLKTVALGTSKINYLDPRITVAWCKRRDVPIEKIFNKSLLAKFAWSMD 322
            RDKETKEDLKTVALGTSKINYLDPRITVAWCKR +VPIEKIFNKSLLAKFAW+M+
Sbjct: 865  RDKETKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMN 919



 Score = 92.0 bits (227), Expect = 9e-16
 Identities = 89/312 (28%), Positives = 146/312 (46%), Gaps = 19/312 (6%)
 Frame = -3

Query: 3066 KANSNHSKIGPNTSASQRPKPHAEDDKPLSSRISTVSKANSNHSKIGPNTSASQRPKPH- 2890
            KA+++ +K  P TS     KP A          +T +    NH K   +++A   PKP  
Sbjct: 91   KASNSSAKASPITSPGANSKPSAS---------ATSTAKQQNHHK---SSAAFIEPKPSV 138

Query: 2889 -----------AEDDKPLSSR-ISTVPKANSNHS-KIGPSTSASQRP----KPQAEDDKP 2761
                       ++D+KPLS+R      K  SNH+ K G  + + +RP       +ED+KP
Sbjct: 139  PAAKANSNSESSDDEKPLSARTFGCSSKGKSNHANKEGGDSCSIRRPVIEESEDSEDEKP 198

Query: 2760 LSSRISPVPKANSNHSKIGPNTSASQRPKPQAEDEKPLSSRISPVPKANSNNSKIGLSTS 2581
            LS R +       + S +G +TS S      ++++KPL++++ P   A S +S +  S S
Sbjct: 199  LSRRFT-------SKSSLGESTSKSY----DSDEKKPLAAKVQPNGSA-SRDSPMNKSAS 246

Query: 2580 ASQRPKPQAEDDKSLSSRISPVPKANSNHSKIGPSMSASQCPKPQVKNEDSDDEKPLSSK 2401
             S +  P   + KSL+      PK +S  + I    +A    KP+ K ED DD  PLS +
Sbjct: 247  LSNKRPPG--EVKSLNQSSLKKPKLSSPITSISNKQAAV---KPEPKAEDDDDNIPLSQR 301

Query: 2400 FTSKSSVGASIGKRDDSDDDKPLSLRVKQN-GSASRDNQSIKPSIGLNKRPPNEERSSGP 2224
               K S  A+   +D+ DD+ P+S R+K++  S S+ +  +K +  +      +      
Sbjct: 302  --GKKSQTATSKPKDEDDDNVPISQRIKKSPASVSKSSAPVKKASTVVSPSLKKVNKKSK 359

Query: 2223 SSVKKPKLSDSS 2188
              +KK   + SS
Sbjct: 360  KVIKKTAYTKSS 371