BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00010650
         (1093 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002263124.1| PREDICTED: gibberellin 3-beta-dioxygenase 4-...   219   8e-77
emb|CAN69444.1| hypothetical protein VITISV_016474 [Vitis vinifera]   219   8e-77
ref|XP_002298993.1| 2-oxoglutarate-dependent dioxygenase [Populu...   205   4e-75
dbj|BAE48659.1| 2-oxoacid-dependent dioxygenase [Prunus mume]         213   1e-73
gb|AFK38139.1| unknown [Lotus japonicus]                              210   4e-73

>ref|XP_002263124.1| PREDICTED: gibberellin 3-beta-dioxygenase 4-like [Vitis vinifera]
          Length = 298

 Score =  219 bits (558), Expect(2) = 8e-77
 Identities = 108/184 (58%), Positives = 134/184 (72%)
 Frame = +1

Query: 394 REVIYKYIKAIRDLAGILGRKLMEGSGLDGDLFDRWCCQLRMNKYHYSPESVGLTGVGIH 573
           RE I  Y KA+++LA  +G KL E  GL GDLF  W CQ R+NKY+++PE+VG  GV IH
Sbjct: 111 RETIKIYAKAVQELAMDIGNKLAETMGLGGDLFKEWPCQFRINKYNFTPETVGSPGVQIH 170

Query: 574 CDPSFLTILQDDENVNGLQVVDEYSGEFVPLDPVPGTLAVNVGDIAKAWSNGRYCNVKHR 753
            D SFLTILQDDENV GL+V+D+ SG FV +DP+PGTL VN+GDIA  WSNGR CNVKHR
Sbjct: 171 TDSSFLTILQDDENVGGLEVMDK-SGAFVAVDPMPGTLLVNLGDIATVWSNGRLCNVKHR 229

Query: 754 VLCFEPKTRCSIAFFVSGPNDKKIEAPSKFVDSGHPRLYAPIDVEEYKQVRISTGLRTGG 933
           V C E   R SIA F+ GP D  +EAP + VDS HPRL+ P+  E+Y+++R+ST L+ G 
Sbjct: 230 VQCKEAAVRLSIASFLLGPKDAAVEAPPELVDSEHPRLFVPVTYEDYRKLRLSTKLQAGE 289

Query: 934 TLDL 945
            L L
Sbjct: 290 ALAL 293



 Score = 95.5 bits (236), Expect(2) = 8e-77
 Identities = 46/105 (43%), Positives = 65/105 (61%), Gaps = 3/105 (2%)
 Frame = +2

Query: 50  VPVIDMTRVEGLGEEIVKACEEWGCFRMVNHGVPVELMAEMKAVASSLFDLPEDVKRQTV 229
           +P IDM         + +ACEEWGCFR+VNH +P  L++EMK+V  SL DLP +VKR+  
Sbjct: 6   IPSIDMQDFPRQFRRLREACEEWGCFRVVNHSIPPSLLSEMKSVVGSLLDLPLEVKRRNT 65

Query: 230 HAEDGKGYVERNQVSPFYEGFSIDEISSS---DEFCDRLHASHHQ 355
               G GY+  ++V+P YE   + +++S    D FC +L AS HQ
Sbjct: 66  DVIAGSGYMAPSKVNPLYEALGLYDMASHQAVDAFCSQLDASPHQ 110


>emb|CAN69444.1| hypothetical protein VITISV_016474 [Vitis vinifera]
          Length = 296

 Score =  219 bits (558), Expect(2) = 8e-77
 Identities = 108/184 (58%), Positives = 134/184 (72%)
 Frame = +1

Query: 394 REVIYKYIKAIRDLAGILGRKLMEGSGLDGDLFDRWCCQLRMNKYHYSPESVGLTGVGIH 573
           RE I  Y KA+++LA  +G KL E  GL GDLF  W CQ R+NKY+++PE+VG  GV IH
Sbjct: 111 RETIKIYAKAVQELAMDIGNKLAETMGLGGDLFKEWPCQFRINKYNFTPETVGSPGVQIH 170

Query: 574 CDPSFLTILQDDENVNGLQVVDEYSGEFVPLDPVPGTLAVNVGDIAKAWSNGRYCNVKHR 753
            D SFLTILQDDENV GL+V+D+ SG FV +DP+PGTL VN+GDIA  WSNGR CNVKHR
Sbjct: 171 TDSSFLTILQDDENVGGLEVMDK-SGAFVAVDPMPGTLLVNLGDIATVWSNGRLCNVKHR 229

Query: 754 VLCFEPKTRCSIAFFVSGPNDKKIEAPSKFVDSGHPRLYAPIDVEEYKQVRISTGLRTGG 933
           V C E   R SIA F+ GP D  +EAP + VDS HPRL+ P+  E+Y+++R+ST L+ G 
Sbjct: 230 VQCKEAAVRLSIASFLLGPKDAAVEAPPELVDSEHPRLFVPVTYEDYRKLRLSTKLQAGE 289

Query: 934 TLDL 945
            L L
Sbjct: 290 ALAL 293



 Score = 95.5 bits (236), Expect(2) = 8e-77
 Identities = 46/105 (43%), Positives = 65/105 (61%), Gaps = 3/105 (2%)
 Frame = +2

Query: 50  VPVIDMTRVEGLGEEIVKACEEWGCFRMVNHGVPVELMAEMKAVASSLFDLPEDVKRQTV 229
           +P IDM         + +ACEEWGCFR+VNH +P  L++EMK+V  SL DLP +VKR+  
Sbjct: 6   IPSIDMQDFPRQFRRLREACEEWGCFRVVNHSIPPSLLSEMKSVVGSLLDLPLEVKRRNT 65

Query: 230 HAEDGKGYVERNQVSPFYEGFSIDEISSS---DEFCDRLHASHHQ 355
               G GY+  ++V+P YE   + +++S    D FC +L AS HQ
Sbjct: 66  DVIAGSGYMAPSKVNPLYEALGLYDMASHQAVDAFCSQLDASPHQ 110


>ref|XP_002298993.1| 2-oxoglutarate-dependent dioxygenase [Populus trichocarpa]
           gi|222846251|gb|EEE83798.1| 2-oxoglutarate-dependent
           dioxygenase [Populus trichocarpa]
          Length = 297

 Score =  205 bits (522), Expect(2) = 4e-75
 Identities = 101/184 (54%), Positives = 135/184 (73%)
 Frame = +1

Query: 394 REVIYKYIKAIRDLAGILGRKLMEGSGLDGDLFDRWCCQLRMNKYHYSPESVGLTGVGIH 573
           REVI  Y KAI  +A  + RKL E  GL GD F+ W CQ R+NKY+++PE++G +GV IH
Sbjct: 111 REVIEMYAKAIHGVAKDVARKLAESLGLSGDFFESWVCQFRINKYNFTPETIGSSGVQIH 170

Query: 574 CDPSFLTILQDDENVNGLQVVDEYSGEFVPLDPVPGTLAVNVGDIAKAWSNGRYCNVKHR 753
            D  FLTILQDD+NV GL+V++  SG +V +DPVPGTL +N+GDIAKAWSNGR+ NV+HR
Sbjct: 171 TDSGFLTILQDDKNVGGLEVMNP-SGVYVAVDPVPGTLLINLGDIAKAWSNGRFRNVQHR 229

Query: 754 VLCFEPKTRCSIAFFVSGPNDKKIEAPSKFVDSGHPRLYAPIDVEEYKQVRISTGLRTGG 933
           V C E   R SIA F+   +D+++EAP + VDS HPRLY P   E+Y+++R++T L+ G 
Sbjct: 230 VQCKEATIRISIASFLLA-SDEEVEAPPELVDSEHPRLYVPFTYEDYRKLRLTTKLQAGE 288

Query: 934 TLDL 945
            L+L
Sbjct: 289 ALEL 292



 Score =  103 bits (257), Expect(2) = 4e-75
 Identities = 49/106 (46%), Positives = 69/106 (65%), Gaps = 3/106 (2%)
 Frame = +2

Query: 47  GVPVIDMTRVEGLGEEIVKACEEWGCFRMVNHGVPVELMAEMKAVASSLFDLPEDVKRQT 226
           GVP ID+    G  E++ +ACEEWGCFR+VNH + + LMA+MK V  SL DLP DVK++ 
Sbjct: 5   GVPTIDLQEFPGQYEKLRRACEEWGCFRLVNHNISLALMADMKRVVRSLLDLPFDVKKRN 64

Query: 227 VHAEDGKGYVERNQVSPFYEGFSIDEISSS---DEFCDRLHASHHQ 355
           +    G GY+  +QV+P YE   + ++ SS   + FC +L AS +Q
Sbjct: 65  LDVISGSGYMAPSQVNPLYEALGLYDMGSSQAVETFCSQLDASSYQ 110


>dbj|BAE48659.1| 2-oxoacid-dependent dioxygenase [Prunus mume]
          Length = 307

 Score =  213 bits (543), Expect(2) = 1e-73
 Identities = 107/190 (56%), Positives = 138/190 (72%), Gaps = 2/190 (1%)
 Frame = +1

Query: 394 REVIYKYIKAIRDLAGILGRKLMEGSGLDG-DLFDRWCCQLRMNKYHYSPESVGLTGVGI 570
           RE+I KY +A+ D    +G KL E  GL+  D  + W CQ R+NKY++ PESVG +GV I
Sbjct: 117 REIISKYAQAVYDQIVEIGHKLAESLGLENVDFLNGWPCQFRINKYNFKPESVGSSGVQI 176

Query: 571 HCDPSFLTILQDDENVNGLQVVDEYSGEFVPLDPVPGTLAVNVGDIAKAWSNGRYCNVKH 750
           H D  FLTILQDDENV GL+V+D+ +G FVP+DP PGTL VN+GD+A+AWSNGR CNV+H
Sbjct: 177 HTDSGFLTILQDDENVGGLEVMDK-AGAFVPVDPCPGTLLVNLGDVAQAWSNGRLCNVRH 235

Query: 751 RVLCFEPKTRCSIAFFVSGPNDKK-IEAPSKFVDSGHPRLYAPIDVEEYKQVRISTGLRT 927
           RV C E   R SIA F+ GP D++ +EAP +FVDS HPRLYAP   E+Y+++R+S+ L+ 
Sbjct: 236 RVQCREATIRVSIATFLLGPKDQEAVEAPPEFVDSEHPRLYAPFTYEDYRKLRLSSKLQA 295

Query: 928 GGTLDLFSTT 957
           G  L L  TT
Sbjct: 296 GEALALLRTT 305



 Score = 90.5 bits (223), Expect(2) = 1e-73
 Identities = 45/107 (42%), Positives = 66/107 (61%), Gaps = 5/107 (4%)
 Frame = +2

Query: 50  VPVIDMTRVEGLGE--EIVKACEEWGCFRMVNHGVPVELMAEMKAVASSLFDLPEDVKRQ 223
           +PVIDM +     E  ++ +A E WGCFR++NH +P+ LM EMKAV  SL DLP ++K+Q
Sbjct: 10  IPVIDMQKFPDAEEYKKLREASEIWGCFRLINHKIPLALMKEMKAVVRSLLDLPMEIKQQ 69

Query: 224 TVHAEDGKGYVERNQVSPFYEGFSIDEISSSD---EFCDRLHASHHQ 355
                 G GY+  ++V+P YE   + ++ S +    FC +L AS HQ
Sbjct: 70  NKDVIAGSGYMAPSKVNPLYEALGLYDLGSPEAVHTFCSKLDASSHQ 116


>gb|AFK38139.1| unknown [Lotus japonicus]
          Length = 300

 Score =  210 bits (535), Expect(2) = 4e-73
 Identities = 97/184 (52%), Positives = 131/184 (71%)
 Frame = +1

Query: 394 REVIYKYIKAIRDLAGILGRKLMEGSGLDGDLFDRWCCQLRMNKYHYSPESVGLTGVGIH 573
           R+++ KY KAI DLA  +G+K+ E  G+ G  F+ W CQ R+NKY+++PE++G  GV +H
Sbjct: 113 RQIMEKYGKAIHDLAAKVGQKMAESLGIQGAGFEDWPCQFRINKYNFTPEAIGSLGVQLH 172

Query: 574 CDPSFLTILQDDENVNGLQVVDEYSGEFVPLDPVPGTLAVNVGDIAKAWSNGRYCNVKHR 753
            D  FLTILQDDENV GL+V+D  SG FVP+ P PG+L  N+GD+A AWSNGR+CNVKHR
Sbjct: 173 TDSGFLTILQDDENVGGLEVMDNDSGSFVPVPPFPGSLLANLGDVAHAWSNGRFCNVKHR 232

Query: 754 VLCFEPKTRCSIAFFVSGPNDKKIEAPSKFVDSGHPRLYAPIDVEEYKQVRISTGLRTGG 933
           V C E   R SIA F+  P +  +EAP + VD  HPRLY P + E+Y+++R+S  +R G 
Sbjct: 233 VQCKEATKRLSIATFLLAPKNGNVEAPEEAVDHDHPRLYQPFNYEDYRKLRLSKKMRAGE 292

Query: 934 TLDL 945
            L+L
Sbjct: 293 ALEL 296



 Score = 92.0 bits (227), Expect(2) = 4e-73
 Identities = 41/107 (38%), Positives = 65/107 (60%), Gaps = 5/107 (4%)
 Frame = +2

Query: 50  VPVIDMTRVEGLGE--EIVKACEEWGCFRMVNHGVPVELMAEMKAVASSLFDLPEDVKRQ 223
           +PV+D  ++    E  ++ +ACE+WGCFR++NH +P  LMAEMK V  +L DLP ++K +
Sbjct: 6   IPVVDFEKISDQVECNKLREACEKWGCFRIINHSIPATLMAEMKTVIGTLLDLPMEIKMR 65

Query: 224 TVHAEDGKGYVERNQVSPFYEGFSIDEISSSD---EFCDRLHASHHQ 355
            +    G GY+  +  +P YE   + ++ SS    +FC +L  S HQ
Sbjct: 66  NIDVAAGSGYMAPSAANPLYEALGLYDLGSSQAVHDFCSQLDVSPHQ 112