BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00010146
         (1038 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFO64617.1| cytochrome P450 mono-oxygenase [Artemisia annua]       516   e-144
emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]                500   e-139
emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]                500   e-139
gb|ABC69377.1| CYP81B2v2 [Nicotiana tabacum]                          415   e-113
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   412   e-113

>gb|AFO64617.1| cytochrome P450 mono-oxygenase [Artemisia annua]
          Length = 502

 Score =  516 bits (1330), Expect = e-144
 Identities = 248/306 (81%), Positives = 271/306 (88%)
 Frame = -1

Query: 1038 EVEEEGKRFRELLDETFLLAGAANVGDYLPILSWLGVKRLEKKLIVLRGRKDVFLQGMIE 859
            EVE EGKRFRE+LDETFLLAGAANVGDYLP L W GV +LEKKL+ LR +++VF QG+IE
Sbjct: 195  EVEAEGKRFREILDETFLLAGAANVGDYLPFLRWFGVTKLEKKLVALREKREVFFQGLIE 254

Query: 858  QLRKAKRGEAENKKKTMIDVLLSLQESDPEYYNDEMIRSLVLVLLSAGTDTTSGTMEWGL 679
            QLRK K  E  N KKTMI+VLLSLQESDP+YY DEMIRS VLVLLSAGTDT++GTMEW +
Sbjct: 255  QLRKPKGAEVGNNKKTMIEVLLSLQESDPKYYTDEMIRSFVLVLLSAGTDTSAGTMEWAM 314

Query: 678  SLLLNNPHVLHKAQNEIDRHVGKDHLIDESDIANLPYLRCIINETLRLYPAAPFLVPHES 499
            SLLLN+P VL KAQNEIDR VG D L+DESD+ NLPYLRCIINETLR+ P  P LVPHES
Sbjct: 315  SLLLNHPQVLKKAQNEIDRVVGNDRLVDESDVVNLPYLRCIINETLRICPPGPLLVPHES 374

Query: 498  SEDCVVGGYNIPRGTMLLVNLWAIHHDPKIWTDPERFKPERFEGLEGTRDGFKLMSFGSG 319
            SEDCV+GGYNIPRGTMLLVN WAIHHDPK+WTDPE FKPERFEGLEGTRDGFKLM FGSG
Sbjct: 375  SEDCVIGGYNIPRGTMLLVNQWAIHHDPKLWTDPEMFKPERFEGLEGTRDGFKLMPFGSG 434

Query: 318  RRSCPGEGLAMRVLGSTLGSLIQCFDWERVSEKMVDMTEGPGLTMPMVEPLVAKCKPRLE 139
            RRSCPGEGLA+RV+GSTLG LIQCFDWER+SEKMVDM+E PGLTMP  EPLVAKCKPRLE
Sbjct: 435  RRSCPGEGLAVRVIGSTLGLLIQCFDWERLSEKMVDMSEAPGLTMPKAEPLVAKCKPRLE 494

Query: 138  MQNLLS 121
            +Q LLS
Sbjct: 495  IQTLLS 500


>emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]
          Length = 505

 Score =  500 bits (1288), Expect = e-139
 Identities = 237/306 (77%), Positives = 275/306 (89%)
 Frame = -1

Query: 1038 EVEEEGKRFRELLDETFLLAGAANVGDYLPILSWLGVKRLEKKLIVLRGRKDVFLQGMIE 859
            E+EEEGKRFR++LDETF+LAGA+NVGDYLP+LSWLGVK LEKKLI L+ ++DVF QG+I+
Sbjct: 198  ELEEEGKRFRDMLDETFVLAGASNVGDYLPVLSWLGVKGLEKKLIKLQEKRDVFFQGLID 257

Query: 858  QLRKAKRGEAENKKKTMIDVLLSLQESDPEYYNDEMIRSLVLVLLSAGTDTTSGTMEWGL 679
            QLRK+K  E  NKKKTMI++LLSLQE++PEYY D MIRS VLVLL+AG+DT++GTMEW +
Sbjct: 258  QLRKSKGTEDVNKKKTMIELLLSLQETEPEYYTDAMIRSFVLVLLAAGSDTSAGTMEWVM 317

Query: 678  SLLLNNPHVLHKAQNEIDRHVGKDHLIDESDIANLPYLRCIINETLRLYPAAPFLVPHES 499
            SLLLN+P VL KAQNEID  +GK+ L+DESDI NLPYLRCIINETLRLYPA P LVPHE+
Sbjct: 318  SLLLNHPQVLKKAQNEIDSVIGKNCLVDESDIPNLPYLRCIINETLRLYPAGPLLVPHEA 377

Query: 498  SEDCVVGGYNIPRGTMLLVNLWAIHHDPKIWTDPERFKPERFEGLEGTRDGFKLMSFGSG 319
            S DCVVGGYN+PRGT+L+VN WAIHHDPK+W +PE FKPERFEGLEGTRDGFKL+ FGSG
Sbjct: 378  SSDCVVGGYNVPRGTILIVNQWAIHHDPKVWDEPETFKPERFEGLEGTRDGFKLLPFGSG 437

Query: 318  RRSCPGEGLAMRVLGSTLGSLIQCFDWERVSEKMVDMTEGPGLTMPMVEPLVAKCKPRLE 139
            RRSCPGEGLA+R+LG TLGS+IQCFDWER SE++VDMTEGPGLTMP   PLVAKCKPR+E
Sbjct: 438  RRSCPGEGLAVRMLGMTLGSIIQCFDWERTSEELVDMTEGPGLTMPKAIPLVAKCKPRVE 497

Query: 138  MQNLLS 121
            M NLLS
Sbjct: 498  MTNLLS 503


>emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]
          Length = 520

 Score =  500 bits (1288), Expect = e-139
 Identities = 237/306 (77%), Positives = 275/306 (89%)
 Frame = -1

Query: 1038 EVEEEGKRFRELLDETFLLAGAANVGDYLPILSWLGVKRLEKKLIVLRGRKDVFLQGMIE 859
            E+EEEGKRFR++LDETF+LAGA+NVGDYLP+LSWLGVK LEKKLI L+ ++DVF QG+I+
Sbjct: 213  ELEEEGKRFRDMLDETFVLAGASNVGDYLPVLSWLGVKGLEKKLIKLQEKRDVFFQGLID 272

Query: 858  QLRKAKRGEAENKKKTMIDVLLSLQESDPEYYNDEMIRSLVLVLLSAGTDTTSGTMEWGL 679
            QLRK+K  E  NKKKTMI++LLSLQE++PEYY D MIRS VLVLL+AG+DT++GTMEW +
Sbjct: 273  QLRKSKGTEDVNKKKTMIELLLSLQETEPEYYTDAMIRSFVLVLLAAGSDTSAGTMEWVM 332

Query: 678  SLLLNNPHVLHKAQNEIDRHVGKDHLIDESDIANLPYLRCIINETLRLYPAAPFLVPHES 499
            SLLLN+P VL KAQNEID  +GK+ L+DESDI NLPYLRCIINETLRLYPA P LVPHE+
Sbjct: 333  SLLLNHPQVLKKAQNEIDSVIGKNCLVDESDIPNLPYLRCIINETLRLYPAGPLLVPHEA 392

Query: 498  SEDCVVGGYNIPRGTMLLVNLWAIHHDPKIWTDPERFKPERFEGLEGTRDGFKLMSFGSG 319
            S DCVVGGYN+PRGT+L+VN WAIHHDPK+W +PE FKPERFEGLEGTRDGFKL+ FGSG
Sbjct: 393  SSDCVVGGYNVPRGTILIVNQWAIHHDPKVWDEPETFKPERFEGLEGTRDGFKLLPFGSG 452

Query: 318  RRSCPGEGLAMRVLGSTLGSLIQCFDWERVSEKMVDMTEGPGLTMPMVEPLVAKCKPRLE 139
            RRSCPGEGLA+R+LG TLGS+IQCFDWER SE++VDMTEGPGLTMP   PLVAKCKPR+E
Sbjct: 453  RRSCPGEGLAVRMLGMTLGSIIQCFDWERTSEELVDMTEGPGLTMPKAIPLVAKCKPRVE 512

Query: 138  MQNLLS 121
            M NLLS
Sbjct: 513  MTNLLS 518


>gb|ABC69377.1| CYP81B2v2 [Nicotiana tabacum]
          Length = 511

 Score =  415 bits (1066), Expect = e-113
 Identities = 199/310 (64%), Positives = 247/310 (79%), Gaps = 7/310 (2%)
 Frame = -1

Query: 1029 EEGKRFRELLDETFLLAGAANVGDYLPILSWLGVKRLEKKLIVLRGRKDVFLQGMIEQLR 850
            EE  RF+ L+ ETF + GA N+GD+LP L  L V++LEK LIVL+  +D F+Q +I+  R
Sbjct: 201  EEATRFKGLVQETFRIGGATNIGDFLPALKLL-VRKLEKSLIVLQENRDEFMQELIKDCR 259

Query: 849  KA--KRG-----EAENKKKTMIDVLLSLQESDPEYYNDEMIRSLVLVLLSAGTDTTSGTM 691
            K   K G     E E  KK +I+VLL+LQE++PEYY DE+IRSL+LVLLSAGTDT+ GTM
Sbjct: 260  KRMEKEGTVTDSEIEGNKKCLIEVLLTLQENEPEYYKDEIIRSLMLVLLSAGTDTSVGTM 319

Query: 690  EWGLSLLLNNPHVLHKAQNEIDRHVGKDHLIDESDIANLPYLRCIINETLRLYPAAPFLV 511
            EW LSL+LN+P  L KAQ EID H+G + L+DESDI NLPYLRCIINET R+YPA P LV
Sbjct: 320  EWALSLMLNHPETLKKAQAEIDEHIGHERLVDESDINNLPYLRCIINETFRMYPAGPLLV 379

Query: 510  PHESSEDCVVGGYNIPRGTMLLVNLWAIHHDPKIWTDPERFKPERFEGLEGTRDGFKLMS 331
            PHESSE+  VGGY +P GTMLLVNLWAIH+DPK+W +P +FKPERF+GL+G RDG+K+M 
Sbjct: 380  PHESSEETTVGGYRVPGGTMLLVNLWAIHNDPKLWDEPRKFKPERFQGLDGVRDGYKMMP 439

Query: 330  FGSGRRSCPGEGLAMRVLGSTLGSLIQCFDWERVSEKMVDMTEGPGLTMPMVEPLVAKCK 151
            FGSGRRSCPGEGLA+R++  +LG +IQCFDW+R+ E++VDMTEG GLT+P  +PLVAKC 
Sbjct: 440  FGSGRRSCPGEGLAVRMVALSLGCIIQCFDWQRIGEELVDMTEGTGLTLPKAQPLVAKCS 499

Query: 150  PRLEMQNLLS 121
            PR +M NLLS
Sbjct: 500  PRPKMANLLS 509


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  412 bits (1058), Expect = e-113
 Identities = 194/308 (62%), Positives = 246/308 (79%), Gaps = 5/308 (1%)
 Frame = -1

Query: 1029 EEGKRFRELLDETFLLAGAANVGDYLPILSWLGVKRLEKKLIVLRGRKDVFLQGMIEQLR 850
            E+ ++F+E+++ETF L+GA+NVGD+LP+L W+G K +EK+LI L+ ++D FLQ +I++ R
Sbjct: 198  EQARKFKEIVEETFELSGASNVGDFLPLLRWIGYKGIEKRLIRLKNKRDEFLQELIQERR 257

Query: 849  K----AKRGEAENKKKTMIDVLLSLQESDPEYYNDEMIRSLVLVLLSAGTDTTSGTMEWG 682
            K    +K   +E KKKT+IDVLLSLQE++PEYY DEMIR L+ +L +AGTDT++GTMEW 
Sbjct: 258  KLRVNSKSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAGTMEWA 317

Query: 681  LSLLLNNPHVLHKAQNEIDRHVGKDHLIDESDIANLPYLRCIINETLRLYPAAPFLVPHE 502
            +SLLLNNP ++ KAQ+EID  +     IDESD+  LPYL CIINETLR+YPA P L+PHE
Sbjct: 318  MSLLLNNPDLIKKAQSEIDLQIEPGRPIDESDLNKLPYLHCIINETLRMYPAGPLLIPHE 377

Query: 501  SSEDCVVGGYNIPRGTMLLVNLWAIHHDPKIWTDPERFKPERFEGLE-GTRDGFKLMSFG 325
            SSEDC +GGYNIP GTMLLVNLWAI +DP +W +P +FKPERFEG + G RDGFKLM FG
Sbjct: 378  SSEDCEIGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKFKPERFEGYQGGVRDGFKLMPFG 437

Query: 324  SGRRSCPGEGLAMRVLGSTLGSLIQCFDWERVSEKMVDMTEGPGLTMPMVEPLVAKCKPR 145
            SGRR CPGEGLA RV+G TL SL+QCFDW RV E+MV+M+EG GLT+P + PL A C+PR
Sbjct: 438  SGRRGCPGEGLAXRVVGLTLXSLLQCFDWHRVGEEMVEMSEGTGLTLPKLHPLEAHCRPR 497

Query: 144  LEMQNLLS 121
              M N LS
Sbjct: 498  STMLNFLS 505