BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00009737
         (1377 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABN79672.1| flavonol synthase [Rudbeckia hirta]                    549   e-154
dbj|BAK09226.1| flavonol synthase [Gentiana triflora]                 492   e-136
sp|Q9M547.1|FLS_EUSGR RecName: Full=Flavonol synthase/flavanone ...   488   e-135
dbj|BAD34463.1| flavonol synthase [Eustoma grandiflorum]              486   e-135
sp|Q07512.1|FLS_PETHY RecName: Full=Flavonol synthase/flavanone ...   479   e-133

>gb|ABN79672.1| flavonol synthase [Rudbeckia hirta]
          Length = 335

 Score =  549 bits (1415), Expect = e-154
 Identities = 266/335 (79%), Positives = 281/335 (83%)
 Frame = +2

Query: 20   MEVERVQEIASLSNLNGIIPSEFIRSENEQPATTTIHGVVLEVPVIDLSHPDDEKLVGSI 199
            MEVERVQEIASLSNL G IP EFIR ENEQPATTTI GVVLEVPVIDLS+ D + LV SI
Sbjct: 1    MEVERVQEIASLSNLKGTIPREFIRLENEQPATTTIQGVVLEVPVIDLSNLDHQSLVASI 60

Query: 200  SEASREWGIFQVVNHGIPSEVITKLQKVGKEFFELPHEEKEAIAKPPGYAGVEGYGTKLQ 379
            SEAS+ WGIFQVVNHGIPSE+I++LQKVGKEFFELP EEKE IAKP GY GVEGYGTKL+
Sbjct: 61   SEASKSWGIFQVVNHGIPSELISELQKVGKEFFELPQEEKEVIAKPIGYQGVEGYGTKLR 120

Query: 380  KEIEGKKGWVDHLFHRVWPPSAINYQFWPKNPPSYRDTNEQYTQMMIEVXXXXXXXXXXX 559
            KE+EGKKGWVDHLFHRVWPPS INY FWPKNPPSYR+ NEQY Q MI V           
Sbjct: 121  KEVEGKKGWVDHLFHRVWPPSTINYHFWPKNPPSYREINEQYAQSMIPVADKLLGLLSQG 180

Query: 560  XXXEEDEVKQGLGGEDLTYMLKINYYPPCPCPELALGVVPHTDMSSITILVPNEVQGLQV 739
               E  ++KQGLGGEDLTYMLKINYYPPCPCPELALGV PHTDMSSITILVPNEVQGLQV
Sbjct: 181  LGLEHGDMKQGLGGEDLTYMLKINYYPPCPCPELALGVAPHTDMSSITILVPNEVQGLQV 240

Query: 740  FRDDHWYDVAYIPNALIVHIGDQLEILSNGMYKSVYHRTTVNKEKTRMSWPMFLEPPPEF 919
            F+DDHWYDVAYIPNALI+HIGDQ+EILSNG YKSVYHRTTVNKEKTRMSWPMFLEPPPEF
Sbjct: 241  FKDDHWYDVAYIPNALIIHIGDQIEILSNGKYKSVYHRTTVNKEKTRMSWPMFLEPPPEF 300

Query: 920  EVGPIPKLINQDNPPXXXXXXXXXXXXXXLNKLPQ 1024
            EVGPIPKLI Q+NP               LNKLPQ
Sbjct: 301  EVGPIPKLITQENPAKYKTKKFKDYVYCKLNKLPQ 335


>dbj|BAK09226.1| flavonol synthase [Gentiana triflora]
          Length = 333

 Score =  492 bits (1266), Expect = e-136
 Identities = 234/335 (69%), Positives = 270/335 (80%)
 Frame = +2

Query: 20   MEVERVQEIASLSNLNGIIPSEFIRSENEQPATTTIHGVVLEVPVIDLSHPDDEKLVGSI 199
            ME +RVQEI+S  N+   IP+E+IRSE EQPA TTIHGVVLEVPVIDLS  D+EK+VG I
Sbjct: 1    MEEKRVQEISS--NVLDTIPAEYIRSEKEQPAITTIHGVVLEVPVIDLSDSDEEKIVGLI 58

Query: 200  SEASREWGIFQVVNHGIPSEVITKLQKVGKEFFELPHEEKEAIAKPPGYAGVEGYGTKLQ 379
            S+AS+EWGIFQVVNHGIP+E I KLQ+VGKEFFELP EEKE IAKP G   +EGYGT+LQ
Sbjct: 59   SQASKEWGIFQVVNHGIPNEAIAKLQEVGKEFFELPQEEKELIAKPEGSQSIEGYGTRLQ 118

Query: 380  KEIEGKKGWVDHLFHRVWPPSAINYQFWPKNPPSYRDTNEQYTQMMIEVXXXXXXXXXXX 559
            KEIEGKKGWVDHLFH++WPPSAINYQFWPKNPP+YR+ NE+Y + +  V           
Sbjct: 119  KEIEGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLHPVVDKMFMYLSLG 178

Query: 560  XXXEEDEVKQGLGGEDLTYMLKINYYPPCPCPELALGVVPHTDMSSITILVPNEVQGLQV 739
               E +++K G GG+DL Y+LKINYYPPCP P+LALGVV HTDMS+ITILVPN+VQGLQV
Sbjct: 179  LDLEPNDLKNGAGGDDLVYLLKINYYPPCPRPDLALGVVAHTDMSAITILVPNDVQGLQV 238

Query: 740  FRDDHWYDVAYIPNALIVHIGDQLEILSNGMYKSVYHRTTVNKEKTRMSWPMFLEPPPEF 919
            F+DDHWYDV YIPNALIVHIGDQ+EI+SNG YK+V+HRTTV KEKTRMSWP+FLEPPPE 
Sbjct: 239  FKDDHWYDVKYIPNALIVHIGDQVEIMSNGKYKAVFHRTTVTKEKTRMSWPVFLEPPPEH 298

Query: 920  EVGPIPKLINQDNPPXXXXXXXXXXXXXXLNKLPQ 1024
            EVGPIPKL+  +NP               LNKLPQ
Sbjct: 299  EVGPIPKLVTDENPAKFKTKKYKDYAYCKLNKLPQ 333


>sp|Q9M547.1|FLS_EUSGR RecName: Full=Flavonol synthase/flavanone 3-hydroxylase; Short=FLS
            gi|7578883|gb|AAF64168.1|AF240764_1 flavonol synthase
            [Eustoma exaltatum subsp. russellianum]
          Length = 334

 Score =  488 bits (1256), Expect = e-135
 Identities = 229/335 (68%), Positives = 269/335 (80%)
 Frame = +2

Query: 20   MEVERVQEIASLSNLNGIIPSEFIRSENEQPATTTIHGVVLEVPVIDLSHPDDEKLVGSI 199
            MEV+RVQEIASLS +   IP+E+IRSENEQP  +T+HGVVLEVPVIDLS  D++K+VG +
Sbjct: 1    MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLV 60

Query: 200  SEASREWGIFQVVNHGIPSEVITKLQKVGKEFFELPHEEKEAIAKPPGYAGVEGYGTKLQ 379
            SEAS+EWGIFQVVNHGIP+EVI KLQ+VGK FFELP EEKE IAKP G   +EGYGT+LQ
Sbjct: 61   SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQ 120

Query: 380  KEIEGKKGWVDHLFHRVWPPSAINYQFWPKNPPSYRDTNEQYTQMMIEVXXXXXXXXXXX 559
            KE++GKKGWVDHLFH++WPPSAINYQFWPKNPP+YR+ NE+Y + +  V           
Sbjct: 121  KEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLG 180

Query: 560  XXXEEDEVKQGLGGEDLTYMLKINYYPPCPCPELALGVVPHTDMSSITILVPNEVQGLQV 739
               E +  K G GG+DL Y++KINYYPPCP P+LALGV  HTDMS+IT+LVPNEV GLQV
Sbjct: 181  LDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGVA-HTDMSAITVLVPNEVPGLQV 239

Query: 740  FRDDHWYDVAYIPNALIVHIGDQLEILSNGMYKSVYHRTTVNKEKTRMSWPMFLEPPPEF 919
            ++D HWYD  YIPNALIVHIGDQ+EI+SNG YKSVYHRTTVNKEKTRMSWP+FLEPPP+ 
Sbjct: 240  YKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDH 299

Query: 920  EVGPIPKLINQDNPPXXXXXXXXXXXXXXLNKLPQ 1024
            EVGPIPKL+N++NP               LNKLPQ
Sbjct: 300  EVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334


>dbj|BAD34463.1| flavonol synthase [Eustoma grandiflorum]
          Length = 335

 Score =  486 bits (1252), Expect = e-135
 Identities = 226/335 (67%), Positives = 269/335 (80%)
 Frame = +2

Query: 20   MEVERVQEIASLSNLNGIIPSEFIRSENEQPATTTIHGVVLEVPVIDLSHPDDEKLVGSI 199
            M+V+RVQEIASLS +   IP+E+IRSENEQ   +T+HGVVLEVPV+DLS  D++K+VG +
Sbjct: 1    MKVQRVQEIASLSKVIDTIPAEYIRSENEQSVISTVHGVVLEVPVVDLSDSDEKKIVGLV 60

Query: 200  SEASREWGIFQVVNHGIPSEVITKLQKVGKEFFELPHEEKEAIAKPPGYAGVEGYGTKLQ 379
            SEAS+EWGIFQVVNHGIP+EVI KLQ+VGK FFELP EEKE IAKP G   +EGYGT+LQ
Sbjct: 61   SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQ 120

Query: 380  KEIEGKKGWVDHLFHRVWPPSAINYQFWPKNPPSYRDTNEQYTQMMIEVXXXXXXXXXXX 559
            KE++GKKGWVDHLFH++WPPS+INYQFWPKNPP+YR+ NE+Y + +  V           
Sbjct: 121  KEVDGKKGWVDHLFHKIWPPSSINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLG 180

Query: 560  XXXEEDEVKQGLGGEDLTYMLKINYYPPCPCPELALGVVPHTDMSSITILVPNEVQGLQV 739
               E +  K G GG+DL Y++KINYYPPCP P+LALGVV HTDMS+IT+LVPNEVQGLQV
Sbjct: 181  LDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGVVAHTDMSAITVLVPNEVQGLQV 240

Query: 740  FRDDHWYDVAYIPNALIVHIGDQLEILSNGMYKSVYHRTTVNKEKTRMSWPMFLEPPPEF 919
            ++D HWYD  YIPNALIVHIGDQ+EI+SNG YKSVYHRTTVNKEKTRMSWP+FLEPPP+ 
Sbjct: 241  YKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDH 300

Query: 920  EVGPIPKLINQDNPPXXXXXXXXXXXXXXLNKLPQ 1024
            EVGPIPKL+N++NP               L KLPQ
Sbjct: 301  EVGPIPKLVNEENPAKFKTKKYKDYAYCKLFKLPQ 335


>sp|Q07512.1|FLS_PETHY RecName: Full=Flavonol synthase/flavanone 3-hydroxylase; Short=FLS
            gi|311658|emb|CAA80264.1| flavonol synthase [Petunia x
            hybrida]
          Length = 348

 Score =  479 bits (1233), Expect = e-133
 Identities = 229/336 (68%), Positives = 264/336 (78%), Gaps = 1/336 (0%)
 Frame = +2

Query: 20   MEVERVQEIASLSNLNGIIPSEFIRSENEQPATTTIHGVVLEVPVIDLSHPDDEKLVGSI 199
            MEV RVQ IASLS     IPSE+IRSENEQPA TT+HGVVL+VPVIDL  PD+ K+V  I
Sbjct: 13   MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 200  SEASREWGIFQVVNHGIPSEVITKLQKVGKEFFE-LPHEEKEAIAKPPGYAGVEGYGTKL 376
            ++AS+EWGIFQ++NHGIP E I  LQKVGKEFFE +P EEKE IAK PG   +EGYGT L
Sbjct: 73   ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSL 132

Query: 377  QKEIEGKKGWVDHLFHRVWPPSAINYQFWPKNPPSYRDTNEQYTQMMIEVXXXXXXXXXX 556
            QKE+EGKKGWVDHLFH++WPPSA+NY++WPKNPPSYR+ NE+Y + M EV          
Sbjct: 133  QKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSL 192

Query: 557  XXXXEEDEVKQGLGGEDLTYMLKINYYPPCPCPELALGVVPHTDMSSITILVPNEVQGLQ 736
                E  E+ +  GG+++ Y+LKINYYPPCP P+LALGVV HTDMS ITILVPNEVQGLQ
Sbjct: 193  GLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQ 252

Query: 737  VFRDDHWYDVAYIPNALIVHIGDQLEILSNGMYKSVYHRTTVNKEKTRMSWPMFLEPPPE 916
            VF+D HWYDV YIPNALIVHIGDQ+EILSNG YKSVYHRTTVNK+KTRMSWP+FLEPP E
Sbjct: 253  VFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSE 312

Query: 917  FEVGPIPKLINQDNPPXXXXXXXXXXXXXXLNKLPQ 1024
             EVGPIPKL+++ NPP              LNKLPQ
Sbjct: 313  HEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348