BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00008119
         (1176 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   382   e-103
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   369   e-100
ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222...   364   2e-98
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              350   4e-94
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   350   4e-94

>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
            communis] gi|223549945|gb|EEF51432.1|
            (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
            [Ricinus communis]
          Length = 496

 Score =  382 bits (980), Expect = e-103
 Identities = 190/381 (49%), Positives = 264/381 (69%), Gaps = 4/381 (1%)
 Frame = +3

Query: 45   SVIWSADCNDYWRSLRTLFRTDIFSPKALESQSGLRTRKLAQMIDFLSSKKGQVVNTEEV 224
            +++W+  CND+W+SLR L RT++FSPKA+ESQ+ LR +KLA+M++FL +K+GQ VN  EV
Sbjct: 120  AIVWNPACNDHWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEV 179

Query: 225  VFTTMFNTLSNIFFGKDFLDLKDHENGTAGGLKGKLFKTLENAVAPNISDFFPMLQRLDL 404
            VF T+FNT+S++ F  D +  ++      GG+K  L+  +E A +PNI++F+P+L  LD 
Sbjct: 180  VFGTIFNTISHLLFSTDLIGFENQ----IGGVKSLLWSMMEMATSPNIAEFYPILAPLDP 235

Query: 405  QGLRKETLKHVKEVHSSWEDIINDRRSLM----AAHKEQCFLDRLLENGFSNDQINTLAL 572
            QGL+++  K +KE+   WE  I +RR       AA K   FLD  L NGF +DQIN L +
Sbjct: 236  QGLKRKMTKCLKEMFGVWEIYIKERRRTHDHDHAAPKTD-FLDIFLSNGFDDDQINWLVM 294

Query: 573  DLFTAGTDTTTSTIVWAMAELIKNKKVMVKVQEELRNKIHSSTIVEPELLSNSKLSYLDA 752
            +L +AGTDTT++++ WAMAEL+KNK+ M KV+EEL  +I+ + I E  +   S+L YL+A
Sbjct: 295  ELLSAGTDTTSTSVEWAMAELLKNKEAMKKVREELDREINKNPIKESHV---SQLPYLNA 351

Query: 753  CIKETXXXXXXXXXXXXXXTIQTCEVMNYTIPDGARIFVNIWAIGRDPKLWEDPLSFKPE 932
            C+KET                + CEVMNYTIP  +++ VN+WAIGRDP +WEDP SFKPE
Sbjct: 352  CVKETLRLHPPAPFLIPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPE 411

Query: 933  RFLSSNLDFRGQDFEFLPFGAGRRMCPGLSSGIKSMQSILSTLILGFDWILPNDEDPMKL 1112
            RFL S+LD +G DFE +PFG+GRR+CPGL    + +  +L++LI  FDW LPN  DP K+
Sbjct: 412  RFLGSSLDVKGHDFELIPFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKV 471

Query: 1113 DMTEKFGVTLHKEKPLHLIFK 1175
            DMTEKFG+TL  E PL +I K
Sbjct: 472  DMTEKFGITLQMEHPLLIIPK 492


>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
            communis] gi|223547255|gb|EEF48750.1|
            (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
            [Ricinus communis]
          Length = 496

 Score =  369 bits (947), Expect = e-100
 Identities = 183/378 (48%), Positives = 254/378 (67%), Gaps = 1/378 (0%)
 Frame = +3

Query: 45   SVIWSADCNDYWRSLRTLFRTDIFSPKALESQSGLRTRKLAQMIDFLSSKKGQVVNTEEV 224
            +++W+  C+D W+ LR + R+++FS KA+ESQ+ LR +KL +M+DFL+SK+GQ+VN  EV
Sbjct: 121  ALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEV 180

Query: 225  VFTTMFNTLSNIFFGKDFLDLKDHENGTAGGLKGKLFKTLENAVAPNISDFFPMLQRLDL 404
            VFTT FNT+SN+ F KD L  +D   G AG LK  +   +E A  PNI+DF+P+L +LD 
Sbjct: 181  VFTTAFNTISNLLFSKDLLSFEDQ--GNAGELKTLISTLMELATCPNIADFYPVLTKLDP 238

Query: 405  QGLRKETLKHVKEVHSSWEDIINDRRSLMAAHKEQC-FLDRLLENGFSNDQINTLALDLF 581
            QG++++    ++ +   W+  I +RR        +  FLD  L NGF + QIN L L+LF
Sbjct: 239  QGIKRKMKNCLERMFGVWDIYIKERRERHVKDARKTDFLDVFLSNGFDDHQINWLLLELF 298

Query: 582  TAGTDTTTSTIVWAMAELIKNKKVMVKVQEELRNKIHSSTIVEPELLSNSKLSYLDACIK 761
            +AG DTTT+T+ WAMAEL+K   V+ KV+EEL  +I    I E  +    +L YL+AC+K
Sbjct: 299  SAGADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIRESHI---PQLKYLNACVK 355

Query: 762  ETXXXXXXXXXXXXXXTIQTCEVMNYTIPDGARIFVNIWAIGRDPKLWEDPLSFKPERFL 941
            ET                + CEVMNYTIP  ++I VN+WAIGRDP  WEDPLS++PERFL
Sbjct: 356  ETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERFL 415

Query: 942  SSNLDFRGQDFEFLPFGAGRRMCPGLSSGIKSMQSILSTLILGFDWILPNDEDPMKLDMT 1121
             SNLDF+G +FEFLPFG+GRR+CPGL  G + +  IL++L+  FDW L N +DP  LDM 
Sbjct: 416  DSNLDFKGHNFEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQNGDDPAMLDMN 475

Query: 1122 EKFGVTLHKEKPLHLIFK 1175
            +KF +TL KE+ L ++ K
Sbjct: 476  DKFSITLEKEQHLLVVPK 493


>ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222859506|gb|EEE97053.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 503

 Score =  364 bits (935), Expect = 2e-98
 Identities = 189/378 (50%), Positives = 257/378 (67%), Gaps = 1/378 (0%)
 Frame = +3

Query: 45   SVIWSADCNDYWRSLRTLFRTDIFSPKALESQSGLRTRKLAQMIDFLSSKKGQVVNTEEV 224
            +++W  +  D W+ LR L RT++FS KA+ES + LR +K+ +++DFL +++G+VV+  EV
Sbjct: 128  ALVWCPESIDTWKLLRGLCRTELFSAKAIESSATLREKKVGELMDFLVAREGKVVSIGEV 187

Query: 225  VFTTMFNTLSNIFFGKDFLDLKDHENGTAGGLKGKLFKTLENAVAPNISDFFPMLQRLDL 404
            VF+T+FNT+SN+ F  D   L+  E G + GLK  + K +     PNI+DF+P+   LD 
Sbjct: 188  VFSTVFNTISNLLFSNDLAGLE--EKGMSSGLKSHVRKLMLLVATPNIADFYPIFAGLDP 245

Query: 405  QGLRKETLKHVKEVHSSWEDIINDRR-SLMAAHKEQCFLDRLLENGFSNDQINTLALDLF 581
            QGLR++  K V+E  + W   I +RR S +    ++ FLD  L NGF +DQIN LA +LF
Sbjct: 246  QGLRRKLSKLVEETFAIWAINIKERRNSYVHDSPKRDFLDVFLANGFDDDQINWLAAELF 305

Query: 582  TAGTDTTTSTIVWAMAELIKNKKVMVKVQEELRNKIHSSTIVEPELLSNSKLSYLDACIK 761
            +AGTDTT +TI WA+AE++KNK+VM KV EEL  +I  +TI E ++   S L YL+ACIK
Sbjct: 306  SAGTDTTATTIEWAVAEILKNKEVMKKVDEELEREITKNTISESDV---SGLPYLNACIK 362

Query: 762  ETXXXXXXXXXXXXXXTIQTCEVMNYTIPDGARIFVNIWAIGRDPKLWEDPLSFKPERFL 941
            ET                +TCEVM YTIP  +++ VN+WAI RDP  WEDPLSFKP+RFL
Sbjct: 363  ETLRLHPPVPLLVPHRATETCEVMKYTIPKDSQVLVNVWAISRDPSTWEDPLSFKPDRFL 422

Query: 942  SSNLDFRGQDFEFLPFGAGRRMCPGLSSGIKSMQSILSTLILGFDWILPNDEDPMKLDMT 1121
             SNL+F+G ++EFLPFGAGRR+CPGL    K +  IL++LI  FDW LPN ED  KLDM 
Sbjct: 423  GSNLEFKGGNYEFLPFGAGRRICPGLPMANKLVPLILASLIRCFDWSLPNGEDLAKLDMK 482

Query: 1122 EKFGVTLHKEKPLHLIFK 1175
            +KFGV L KE+PL L+ K
Sbjct: 483  DKFGVVLQKEQPLVLVPK 500


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  350 bits (898), Expect = 4e-94
 Identities = 173/378 (45%), Positives = 254/378 (67%), Gaps = 3/378 (0%)
 Frame = +3

Query: 45   SVIWSADCNDYWRSLRTLFRTDIFSPKALESQSGLRTRKLAQMIDFLSSKKGQVVNTEEV 224
            S++++ DCN  W++LR + R ++FS KA+ESQ  LR RK+ ++++FL++K+G+VV   ++
Sbjct: 662  SIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDL 721

Query: 225  VFTTMFNTLSNIFFGKDFLDLKDHENGTAGGLKGKLFKTLENAVAPNISDFFPMLQRLDL 404
            VFTT+ N LSN FF  D  D +D E    G LK  + K  E    PN+SD++P+L  LD+
Sbjct: 722  VFTTICNILSNKFFSMDLCDFED-EGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDI 780

Query: 405  QGLRKETLKHVKEVHSSWEDIINDRR---SLMAAHKEQCFLDRLLENGFSNDQINTLALD 575
            QG+ ++  +  + + ++WEDI+ +RR   S  ++H++  FL+ LLE GF +DQIN + L+
Sbjct: 781  QGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRD--FLEALLEIGFEDDQINQVILE 838

Query: 576  LFTAGTDTTTSTIVWAMAELIKNKKVMVKVQEELRNKIHSSTIVEPELLSNSKLSYLDAC 755
            LF+AG +T++ T+ WAMAELI+N+  M K++ ELR  +  S + E  L    +L YL AC
Sbjct: 839  LFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHL---PRLPYLQAC 895

Query: 756  IKETXXXXXXXXXXXXXXTIQTCEVMNYTIPDGARIFVNIWAIGRDPKLWEDPLSFKPER 935
            +KE                 +TC+VM YTIP  ++IFVNIWA+ RDPK+W+DPLSFKPER
Sbjct: 896  VKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPER 955

Query: 936  FLSSNLDFRGQDFEFLPFGAGRRMCPGLSSGIKSMQSILSTLILGFDWILPNDEDPMKLD 1115
            FL S LDF+G DFE++PFGAGRR+CPGL+ G + +  IL+T +  F W LP + D  +LD
Sbjct: 956  FLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLD 1015

Query: 1116 MTEKFGVTLHKEKPLHLI 1169
            M E   +TL KE+PL L+
Sbjct: 1016 MEEWLVITLRKEQPLRLV 1033



 Score =  330 bits (847), Expect = 3e-88
 Identities = 164/380 (43%), Positives = 250/380 (65%), Gaps = 3/380 (0%)
 Frame = +3

Query: 45   SVIWSADCNDYWRSLRTLFRTDIFSPKALESQSGLRTRKLAQMIDFLSSKKGQVVNTEEV 224
            S+ ++ +C + W++LRT+ RT++FS KA+ES   LR RK+ ++++FL++K+G+VV   ++
Sbjct: 1167 SLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDL 1226

Query: 225  VFTTMFNTLSNIFFGKDFLDLKDHENGTAGGLKGKLFKTLENAVAPNISDFFPMLQRLDL 404
            VFTT+ N LSN FF  D  D +        GLK  +++  E    PN+SDF+P+L  L+L
Sbjct: 1227 VFTTICNILSNTFFSMDLCDFERE------GLKDFIYRAAELGATPNLSDFYPILDGLNL 1280

Query: 405  QGLRKETLKHVKEVHSSWEDIINDRRSLM---AAHKEQCFLDRLLENGFSNDQINTLALD 575
             G +K++ + +  + ++WE  + +RR      ++H++   L+  LE  F +DQIN + L+
Sbjct: 1281 HGSKKKSKEALGRILATWEGTLKERRKQKNPGSSHRD--LLEAFLEIRFEDDQINQVILE 1338

Query: 576  LFTAGTDTTTSTIVWAMAELIKNKKVMVKVQEELRNKIHSSTIVEPELLSNSKLSYLDAC 755
            LF+AG DT+T TI WA+ +LI+N  VM K+++EL   I  S + E  L     L YL AC
Sbjct: 1339 LFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHL---PHLPYLQAC 1395

Query: 756  IKETXXXXXXXXXXXXXXTIQTCEVMNYTIPDGARIFVNIWAIGRDPKLWEDPLSFKPER 935
            +KET               ++TC+VM YTIP  +++FVNIWA+GRDPK+W+DPLSF PER
Sbjct: 1396 VKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPER 1455

Query: 936  FLSSNLDFRGQDFEFLPFGAGRRMCPGLSSGIKSMQSILSTLILGFDWILPNDEDPMKLD 1115
            FL S L+F+G DFE++PFGAGRR+CPG++ G + +  +L+TL+  FDW LP++ D  ++D
Sbjct: 1456 FLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQID 1515

Query: 1116 MTEKFGVTLHKEKPLHLIFK 1175
            M E   +TL KE PL L+ K
Sbjct: 1516 MEEWLVITLRKENPLRLVPK 1535



 Score =  327 bits (839), Expect = 3e-87
 Identities = 173/377 (45%), Positives = 243/377 (64%), Gaps = 7/377 (1%)
 Frame = +3

Query: 66   CNDYWRSLRTLFRTDIFSPKALESQSGLRTRKLAQMIDFLSSKKGQVVNTEEVVFTTMFN 245
            CN  WR+L+ + R ++FS KA+ES+  LR RK+ ++++FL  K+G+VV   ++V+TT+ N
Sbjct: 129  CNSDWRNLKAICRMELFSGKAMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCN 188

Query: 246  TLSNIFFGKDFLDLKDHENGTAGGLKGKLFKTL--ENAV--APNISDFFPMLQRLDLQGL 413
             LSN FF  DF D +  +      ++G L K L  ENA   A NI DF+P+L  LD+QG+
Sbjct: 189  ILSNKFFSIDFSDFEGRD------VRGVLLKDLLNENAELGATNILDFYPILGGLDIQGI 242

Query: 414  RKETLKHVKEVHSSWEDIINDRRSLM---AAHKEQCFLDRLLENGFSNDQINTLALDLFT 584
            RK+  +  + + ++WEDI+ +RR      ++H +  FLD LLE GF +DQIN + ++LF 
Sbjct: 243  RKKLKEIFRRIPTTWEDILKERRKQRIHGSSHGD--FLDALLETGFEDDQINHVIMELFF 300

Query: 585  AGTDTTTSTIVWAMAELIKNKKVMVKVQEELRNKIHSSTIVEPELLSNSKLSYLDACIKE 764
            AG +T++ T+ WAMAELIKN+  M K+  EL   I  S + E  L     L YL AC+KE
Sbjct: 301  AGPETSSLTVEWAMAELIKNQDAMHKLCNELTQIIGESPVRESHL---PHLPYLQACVKE 357

Query: 765  TXXXXXXXXXXXXXXTIQTCEVMNYTIPDGARIFVNIWAIGRDPKLWEDPLSFKPERFLS 944
            T                +TC++M YTIP  + IFVN+WA+GRDP  WEDPLSFKPERFL 
Sbjct: 358  TLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWEDPLSFKPERFLD 417

Query: 945  SNLDFRGQDFEFLPFGAGRRMCPGLSSGIKSMQSILSTLILGFDWILPNDEDPMKLDMTE 1124
            S L+F+G DFE++PFGAGRRMCPG+    + +  IL+T +  FDW  P D D  ++DM E
Sbjct: 418  SKLEFKGNDFEYIPFGAGRRMCPGMPLAARLVPMILATFVRLFDWSTPGDMDFAEIDMEE 477

Query: 1125 KFGVTLHKEKPLHLIFK 1175
            +F +TL KE+PL L+ K
Sbjct: 478  RFVITLRKEQPLRLLKK 494


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Vitis vinifera]
          Length = 498

 Score =  350 bits (898), Expect = 4e-94
 Identities = 173/378 (45%), Positives = 254/378 (67%), Gaps = 3/378 (0%)
 Frame = +3

Query: 45   SVIWSADCNDYWRSLRTLFRTDIFSPKALESQSGLRTRKLAQMIDFLSSKKGQVVNTEEV 224
            S++++ DCN  W++LR + R ++FS KA+ESQ  LR RK+ ++++FL++K+G+VV   ++
Sbjct: 122  SIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDL 181

Query: 225  VFTTMFNTLSNIFFGKDFLDLKDHENGTAGGLKGKLFKTLENAVAPNISDFFPMLQRLDL 404
            VFTT+ N LSN FF  D  D +D E    G LK  + K  E    PN+SD++P+L  LD+
Sbjct: 182  VFTTICNILSNKFFSMDLCDFED-EGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDI 240

Query: 405  QGLRKETLKHVKEVHSSWEDIINDRR---SLMAAHKEQCFLDRLLENGFSNDQINTLALD 575
            QG+ ++  +  + + ++WEDI+ +RR   S  ++H++  FL+ LLE GF +DQIN + L+
Sbjct: 241  QGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRD--FLEALLEIGFEDDQINQVILE 298

Query: 576  LFTAGTDTTTSTIVWAMAELIKNKKVMVKVQEELRNKIHSSTIVEPELLSNSKLSYLDAC 755
            LF+AG +T++ T+ WAMAELI+N+  M K++ ELR  +  S + E  L    +L YL AC
Sbjct: 299  LFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHL---PRLPYLQAC 355

Query: 756  IKETXXXXXXXXXXXXXXTIQTCEVMNYTIPDGARIFVNIWAIGRDPKLWEDPLSFKPER 935
            +KE                 +TC+VM YTIP  ++IFVNIWA+ RDPK+W+DPLSFKPER
Sbjct: 356  VKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPER 415

Query: 936  FLSSNLDFRGQDFEFLPFGAGRRMCPGLSSGIKSMQSILSTLILGFDWILPNDEDPMKLD 1115
            FL S LDF+G DFE++PFGAGRR+CPGL+ G + +  IL+T +  F W LP + D  +LD
Sbjct: 416  FLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLD 475

Query: 1116 MTEKFGVTLHKEKPLHLI 1169
            M E   +TL KE+PL L+
Sbjct: 476  MEEWLVITLRKEQPLRLV 493