BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00007543
         (1817 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAJ17664.1| 4-coumarate:CoA ligase [Gynura bicolor]               989   0.0  
gb|AFL93685.1| 4-coumarate: coenzyme A ligase [Cynara cardunculu...   964   0.0  
gb|AEM63673.1| 4-hydroxycinnamoyl CoA ligase [Platycodon grandif...   926   0.0  
sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; ...   910   0.0  
dbj|BAA07828.1| 4-coumarate:coenzyme A ligase [Nicotiana tabacum]     905   0.0  

>dbj|BAJ17664.1| 4-coumarate:CoA ligase [Gynura bicolor]
          Length = 542

 Score =  989 bits (2557), Expect = 0.0
 Identities = 491/538 (91%), Positives = 521/538 (96%)
 Frame = +3

Query: 66   PEKEIIFRSKLPDIYIPKHLTLHSYCFENISSFSDRPCLIDGATGQVYTYADVELTSRKV 245
            PEKEIIFRSKLPDIYIPKHL LHSYCFENIS FSDRPCLIDGA+GQ YTYADVELTSRKV
Sbjct: 5    PEKEIIFRSKLPDIYIPKHLPLHSYCFENISKFSDRPCLIDGASGQEYTYADVELTSRKV 64

Query: 246  ASALHKQGINKGDVIMILLPNSAEFVFSFLGASYLGAISTMANPFFTAAEIIKQAKASSA 425
            ASALH+QGINKGDVIMILLPNS EFV++F+GASYLGAISTMANPFFTAAEIIKQAKAS+A
Sbjct: 65   ASALHQQGINKGDVIMILLPNSPEFVYAFIGASYLGAISTMANPFFTAAEIIKQAKASNA 124

Query: 426  KIIITQSSHVPKVKDFAADNSIKVVCIDSAPEGCLHYSELTSGDETELPEVEISSDDVVA 605
            KIIITQS+HV KVK F+++NSI +VCIDSAPEGCLHYSEL SGDE++LPEVEIS DDVVA
Sbjct: 125  KIIITQSAHVSKVKRFSSENSITIVCIDSAPEGCLHYSELLSGDESKLPEVEISPDDVVA 184

Query: 606  LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVVICVLPLFHIYSLNSIL 785
            LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDV+ICVLPLFHIYSLNSIL
Sbjct: 185  LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLICVLPLFHIYSLNSIL 244

Query: 786  LCGLRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEEVVDKYDLSSMRT 965
            LCGLRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNE++VDKYDLSS+RT
Sbjct: 245  LCGLRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEKIVDKYDLSSIRT 304

Query: 966  VMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVV 1145
            VMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEP+DIKSGACGTVV
Sbjct: 305  VMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYDIKSGACGTVV 364

Query: 1146 RNAEMKIVDPETGASLPRNQRGEICIRGDQIMKGYVDDPEATKRTIDSEGWLHTGDIGYI 1325
            RNAEMKIVDP+TG SLPRNQRGEICIRGDQIMKGY++DPE+TK TID++GWLHTGDIG I
Sbjct: 365  RNAEMKIVDPDTGLSLPRNQRGEICIRGDQIMKGYLNDPESTKNTIDADGWLHTGDIGLI 424

Query: 1326 DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMINEAAGEVPVAFVV 1505
            DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVV M+N+AAGEVPVAFVV
Sbjct: 425  DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVSMVNDAAGEVPVAFVV 484

Query: 1506 KSNGSGVTEDDIKQFISKQVVFYKRLKRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 1679
            K+N S VTED+IKQF+SKQVVFYKR+ RVFFID IPKSPSGKILRKDLRAKLAAGVPN
Sbjct: 485  KTNDSSVTEDEIKQFVSKQVVFYKRINRVFFIDTIPKSPSGKILRKDLRAKLAAGVPN 542


>gb|AFL93685.1| 4-coumarate: coenzyme A ligase [Cynara cardunculus var. scolymus]
          Length = 539

 Score =  964 bits (2492), Expect = 0.0
 Identities = 483/540 (89%), Positives = 514/540 (95%)
 Frame = +3

Query: 60   MEPEKEIIFRSKLPDIYIPKHLTLHSYCFENISSFSDRPCLIDGATGQVYTYADVELTSR 239
            ME + E IFRS+LPDIYIPKHL+LHSYCFENIS+FSDRPCLIDGATG VYTYADVELTSR
Sbjct: 1    MEAKSETIFRSRLPDIYIPKHLSLHSYCFENISAFSDRPCLIDGATGDVYTYADVELTSR 60

Query: 240  KVASALHKQGINKGDVIMILLPNSAEFVFSFLGASYLGAISTMANPFFTAAEIIKQAKAS 419
            KV SALHK GINKGDVIMILLPNS EFV+SFLGAS++GA+STMANPFFT+AEIIKQAKAS
Sbjct: 61   KVGSALHKHGINKGDVIMILLPNSPEFVYSFLGASFIGAVSTMANPFFTSAEIIKQAKAS 120

Query: 420  SAKIIITQSSHVPKVKDFAADNSIKVVCIDSAPEGCLHYSELTSGDETELPEVEISSDDV 599
            +AKIIITQS H  KVKDFA++NSIK+VCIDSAPE CLH+SEL SGDE ELPEVEISSDDV
Sbjct: 121  AAKIIITQSEHAAKVKDFASENSIKLVCIDSAPEECLHFSELISGDEEELPEVEISSDDV 180

Query: 600  VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVVICVLPLFHIYSLNS 779
            VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDV++CVLPLFHIYSLNS
Sbjct: 181  VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLMCVLPLFHIYSLNS 240

Query: 780  ILLCGLRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEEVVDKYDLSSM 959
            ILLCGLRAGAAILIMQKF IVPFL+LI+KYKVTIGPFVPPIVLTIA NEEVVDKYDL+S+
Sbjct: 241  ILLCGLRAGAAILIMQKFHIVPFLELIQKYKVTIGPFVPPIVLTIAKNEEVVDKYDLTSI 300

Query: 960  RTVMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 1139
            RTVM GAAPLGKELE+TVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF+IKSGACGT
Sbjct: 301  RTVMFGAAPLGKELENTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGT 360

Query: 1140 VVRNAEMKIVDPETGASLPRNQRGEICIRGDQIMKGYVDDPEATKRTIDSEGWLHTGDIG 1319
            VVRNAEMKIVDP+TG SLPRNQRGEICIRGDQIMKGY++DPEATK TI  +GWLHTGDIG
Sbjct: 361  VVRNAEMKIVDPDTGVSLPRNQRGEICIRGDQIMKGYLNDPEATKSTI-VDGWLHTGDIG 419

Query: 1320 YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMINEAAGEVPVAF 1499
            YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPM +E AGEVPVAF
Sbjct: 420  YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMKHEDAGEVPVAF 479

Query: 1500 VVKSNGSGVTEDDIKQFISKQVVFYKRLKRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 1679
            VVKSNG+ VTE D+KQFISKQVVFYK+L RVFFID +PKSPSGKILRKDLRAKLAAGVPN
Sbjct: 480  VVKSNGTTVTEVDVKQFISKQVVFYKKLYRVFFIDKVPKSPSGKILRKDLRAKLAAGVPN 539


>gb|AEM63673.1| 4-hydroxycinnamoyl CoA ligase [Platycodon grandiflorus]
          Length = 556

 Score =  926 bits (2394), Expect = 0.0
 Identities = 462/548 (84%), Positives = 507/548 (92%)
 Frame = +3

Query: 36   PENQEYSQMEPEKEIIFRSKLPDIYIPKHLTLHSYCFENISSFSDRPCLIDGATGQVYTY 215
            P   E S    ++EIIFRSKLPDIYIP HL LHSYCFENIS FS+RPCLI+GATG+VYTY
Sbjct: 10   PITTEESLPAVKQEIIFRSKLPDIYIPNHLPLHSYCFENISKFSERPCLIEGATGKVYTY 69

Query: 216  ADVELTSRKVASALHKQGINKGDVIMILLPNSAEFVFSFLGASYLGAISTMANPFFTAAE 395
            ADVE+TSRKVAS+L+K GI +GD IM+LLPNSAEFVF+FLGASYLGAISTMANPFFT AE
Sbjct: 70   ADVEVTSRKVASSLNKLGIGQGDTIMLLLPNSAEFVFAFLGASYLGAISTMANPFFTHAE 129

Query: 396  IIKQAKASSAKIIITQSSHVPKVKDFAADNSIKVVCIDSAPEGCLHYSELTSGDETELPE 575
            IIKQ KAS+AKIIIT + HV KVK+FA++N +KV+CID APEGCLHYSELT GDE +LPE
Sbjct: 130  IIKQVKASNAKIIITLACHVDKVKEFASENDVKVICIDKAPEGCLHYSELTLGDENDLPE 189

Query: 576  VEISSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVVICVLPL 755
            V+IS DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NL++HSEDV+ICVLPL
Sbjct: 190  VKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLICVLPL 249

Query: 756  FHIYSLNSILLCGLRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEEVV 935
            FHIYSLNSILLCGLR GAAILIMQKFDIVPFL+LIEKYKVTIGPFVPPIVL +A +   V
Sbjct: 250  FHIYSLNSILLCGLRVGAAILIMQKFDIVPFLELIEKYKVTIGPFVPPIVLAMAKSSH-V 308

Query: 936  DKYDLSSMRTVMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 1115
            DKYDL+S+RTVMSGAAPLGKELEDTVR K PNAKLGQGYGMTEAGPVLAMCLAFAKEP++
Sbjct: 309  DKYDLTSIRTVMSGAAPLGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPYE 368

Query: 1116 IKSGACGTVVRNAEMKIVDPETGASLPRNQRGEICIRGDQIMKGYVDDPEATKRTIDSEG 1295
            IKSGACGTVVRNAEMKIVDP+TG SLPRNQRGEICIRGDQIMKGY++DPEAT+RTID EG
Sbjct: 369  IKSGACGTVVRNAEMKIVDPDTGDSLPRNQRGEICIRGDQIMKGYLNDPEATERTIDKEG 428

Query: 1296 WLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMINEA 1475
            WLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLL HP+ISDAAVV MI+EA
Sbjct: 429  WLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVSMIDEA 488

Query: 1476 AGEVPVAFVVKSNGSGVTEDDIKQFISKQVVFYKRLKRVFFIDAIPKSPSGKILRKDLRA 1655
            AGEVPVAFVV++NGS +TED+IKQFISKQVVFYKR+ RVFF D IPK+PSGKILRK+LRA
Sbjct: 489  AGEVPVAFVVRANGSIITEDEIKQFISKQVVFYKRINRVFFTDTIPKAPSGKILRKELRA 548

Query: 1656 KLAAGVPN 1679
            +LAAG+PN
Sbjct: 549  RLAAGIPN 556


>sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName:
            Full=4-coumaroyl-CoA synthase 2 gi|1663724|gb|AAB18638.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 542

 Score =  910 bits (2351), Expect = 0.0
 Identities = 449/535 (83%), Positives = 493/535 (92%)
 Frame = +3

Query: 75   EIIFRSKLPDIYIPKHLTLHSYCFENISSFSDRPCLIDGATGQVYTYADVELTSRKVASA 254
            +IIFRSKLPDIYIP HL LHSYCFENIS FS RPCLI+GA  Q+YTYADVEL SRKVA+ 
Sbjct: 9    DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 255  LHKQGINKGDVIMILLPNSAEFVFSFLGASYLGAISTMANPFFTAAEIIKQAKASSAKII 434
            LHKQGI   D IMILLPNS EFVF+F+GASYLGAISTMANP FT AE++KQAKASSAKII
Sbjct: 69   LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 435  ITQSSHVPKVKDFAADNSIKVVCIDSAPEGCLHYSELTSGDETELPEVEISSDDVVALPY 614
            +TQ+ HV KVKD+A +N +K++CIDSAPEGCLH+S LT  +E ++PEVEI  DDVVALPY
Sbjct: 129  VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 615  SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVVICVLPLFHIYSLNSILLCG 794
            SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNL+IHSEDV++CVLPLFHIYSLNS+LLCG
Sbjct: 189  SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 795  LRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEEVVDKYDLSSMRTVMS 974
            LR GAAILIMQKFDIV FL+LI++YKVTIGPFVPPIVL IA +  +VD YDLSS+RTVMS
Sbjct: 249  LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKS-PMVDDYDLSSVRTVMS 307

Query: 975  GAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNA 1154
            GAAPLGKELEDTVR KFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF+IKSGACGTVVRNA
Sbjct: 308  GAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNA 367

Query: 1155 EMKIVDPETGASLPRNQRGEICIRGDQIMKGYVDDPEATKRTIDSEGWLHTGDIGYIDDD 1334
            EMKIVDP+TG SLPRNQ GEICIRGDQIMKGY++DPEAT RTID EGWL+TGDIGYIDDD
Sbjct: 368  EMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDD 427

Query: 1335 DELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMINEAAGEVPVAFVVKSN 1514
            DELFIVDRLKELIKYKGFQVAPAELEALLL HP+ISDAAVVPM +E AGEVPVAFVV+SN
Sbjct: 428  DELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSN 487

Query: 1515 GSGVTEDDIKQFISKQVVFYKRLKRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 1679
            GS +TED++K FISKQV+FYKR+KRVFF+DAIPKSPSGKILRKDLRAKLAAG+PN
Sbjct: 488  GSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542


>dbj|BAA07828.1| 4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 542

 Score =  905 bits (2339), Expect = 0.0
 Identities = 446/535 (83%), Positives = 491/535 (91%)
 Frame = +3

Query: 75   EIIFRSKLPDIYIPKHLTLHSYCFENISSFSDRPCLIDGATGQVYTYADVELTSRKVASA 254
            +IIFRSKLPDIYIP HL LHSYCFENIS FS RPCLI+GA  Q+YTYADVEL+SRKVA+ 
Sbjct: 9    DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELSSRKVAAG 68

Query: 255  LHKQGINKGDVIMILLPNSAEFVFSFLGASYLGAISTMANPFFTAAEIIKQAKASSAKII 434
            LHKQGI + D IMILLPNS EFVF+F+GASYLGAISTMANP FTAAE++KQ KAS AKII
Sbjct: 69   LHKQGIQQKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTAAEVVKQVKASGAKII 128

Query: 435  ITQSSHVPKVKDFAADNSIKVVCIDSAPEGCLHYSELTSGDETELPEVEISSDDVVALPY 614
            +TQ+ HV KVKD+A +N++K++CIDSAPEGCLH+S LT  DE ++PEVEI  DDVVALPY
Sbjct: 129  VTQACHVNKVKDYALENNVKIICIDSAPEGCLHFSVLTQADEHDIPEVEIQPDDVVALPY 188

Query: 615  SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVVICVLPLFHIYSLNSILLCG 794
            SSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NL+IHSEDV++CVLPLFHIYSLNS+LLCG
Sbjct: 189  SSGTTGLPKGVMLTHKGLVTSVAQQVDGENRNLYIHSEDVLLCVLPLFHIYSLNSVLLCG 248

Query: 795  LRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEEVVDKYDLSSMRTVMS 974
            LR GAAILIMQKFDIVPFL+LI+ YKVTIGPFVPPIVL IA +  +VD YDLSS+RTVMS
Sbjct: 249  LRVGAAILIMQKFDIVPFLELIQNYKVTIGPFVPPIVLAIAKS-PMVDDYDLSSVRTVMS 307

Query: 975  GAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNA 1154
            GAAPLGKELEDTVR KFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF+IKSGACGTVVRNA
Sbjct: 308  GAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNA 367

Query: 1155 EMKIVDPETGASLPRNQRGEICIRGDQIMKGYVDDPEATKRTIDSEGWLHTGDIGYIDDD 1334
            EMKIVDPETG SLPRNQ GEICIRGDQIMKGY++DPEAT RTID EGWL+TGDIGYIDDD
Sbjct: 368  EMKIVDPETGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDD 427

Query: 1335 DELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMINEAAGEVPVAFVVKSN 1514
            DELFIVDRLKELIKYKGFQVAPAELEALLL HP  SDAAVVPM +E A EVPVAFVV+S+
Sbjct: 428  DELFIVDRLKELIKYKGFQVAPAELEALLLNHPTFSDAAVVPMKDEQAEEVPVAFVVRSS 487

Query: 1515 GSGVTEDDIKQFISKQVVFYKRLKRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 1679
            GS +TED++K FISKQV+FYKR+KRVFF+DA+PKSPSGKILRKDLRAKLAAG+PN
Sbjct: 488  GSTITEDEVKDFISKQVIFYKRIKRVFFVDAVPKSPSGKILRKDLRAKLAAGLPN 542