BLASTX nr result
ID: Atractylodes21_contig00004837
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes21_contig00004837
(975 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003551333.1| PREDICTED: neutral ceramidase-like [Glycine ... 124 4e-41
ref|XP_003530830.1| PREDICTED: neutral ceramidase-like [Glycine ... 120 7e-40
ref|XP_003519651.1| PREDICTED: neutral ceramidase-like [Glycine ... 115 7e-40
ref|XP_002279962.2| PREDICTED: neutral ceramidase-like [Vitis vi... 107 1e-39
ref|XP_003617915.1| Neutral ceramidase [Medicago truncatula] gi|... 114 1e-38
>ref|XP_003551333.1| PREDICTED: neutral ceramidase-like [Glycine max]
Length = 779
Score = 124 bits (312), Expect(4) = 4e-41
Identities = 113/306 (36%), Positives = 135/306 (44%), Gaps = 67/306 (21%)
Frame = -1
Query: 771 GNAFWKLVRNVLKTPSKEQTDRQQPMPMLV*LGE-------------------------- 670
GN FWKLVR++LKTPSKEQTD Q+P P+L+ GE
Sbjct: 479 GNPFWKLVRDMLKTPSKEQTDCQRPKPILLDTGEMKKPYDWAPSILPIQILRIGQLIILS 538
Query: 669 ----FTTMAGRCLRDAVFKEKVCRNSRS-----D*YLLPI-----------CY*R*GVHD 550
FTTMAGR LRDAV K S D +++ Y V
Sbjct: 539 VPGEFTTMAGRRLRDAV---KTVLTSEEYFEFDDIHIVIAGLTNTYSQYITTYEEYQVQR 595
Query: 549 AE----IRGSFNSILSVPTFRSW*QPLASGKPVEAGPQQPDLLNKQISLLPPVVVDSTPL 382
E + G + F+ + L G+PVE GPQ PDLL KQISLLPPVVVD+TPL
Sbjct: 596 YEGASTLYGPHTLSAYIQEFKKLAEALVYGEPVEPGPQPPDLLEKQISLLPPVVVDATPL 655
Query: 381 GVK---VPTSPV*LHLQDQGDAVTVVFWSACPRNDLSLL*RSCFRGKIRGSRHTIFAFVS 211
GV V T GD VT FWSACPRNDL ++ A+
Sbjct: 656 GVNFGDVCTDVPRNSTFKSGDLVTASFWSACPRNDLMTEGTFALVEFLQEKDAWTPAYDD 715
Query: 210 SGRGLQNSALRVMLP*NGKSRNRLCLEFT--------------EGASKSLFRSISHFTGS 73
L+ R P SR++ LE+ GA+K LF SI HFTGS
Sbjct: 716 DDFCLRYKWSR---PSKLSSRSKATLEWRIPQSVAPGVYRLRHFGAAKGLFGSIHHFTGS 772
Query: 72 SNVFVV 55
S FVV
Sbjct: 773 STAFVV 778
Score = 66.2 bits (160), Expect(4) = 4e-41
Identities = 30/51 (58%), Positives = 40/51 (78%)
Frame = -2
Query: 947 ERQFKKAAELFNGASDKLNGKIDFRHTYLDFSQLEVALAKQGGGSETVKSC 795
ERQF+KA +LFN A +++ G +DFRH Y+DFSQLEV ++ Q G SE VK+C
Sbjct: 402 ERQFRKAVDLFNAADEEIEGGVDFRHAYIDFSQLEVTISDQ-GYSEVVKTC 451
Score = 23.9 bits (50), Expect(4) = 4e-41
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -3
Query: 820 EGVRLLKVAMGFAFAAG 770
E V+ AMGFAFAAG
Sbjct: 446 EVVKTCPAAMGFAFAAG 462
Score = 21.2 bits (43), Expect(4) = 4e-41
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = -1
Query: 975 DEFESTCIFGE 943
DEFEST I GE
Sbjct: 392 DEFESTRIIGE 402
>ref|XP_003530830.1| PREDICTED: neutral ceramidase-like [Glycine max]
Length = 778
Score = 120 bits (300), Expect(4) = 7e-40
Identities = 109/310 (35%), Positives = 134/310 (43%), Gaps = 71/310 (22%)
Frame = -1
Query: 771 GNAFWKLVRNVLKTPSKEQTDRQQPMPMLV*LGE-------------------------- 670
GN FWKLVR++LKTPS+EQ D Q P P+L+ GE
Sbjct: 478 GNPFWKLVRDMLKTPSREQIDCQHPKPILLDTGEMKKPYDWAPSILPIQILRIGQLIILS 537
Query: 669 ----FTTMAGRCLRDAVFKEKVCRNSRSD*YLLPICY*R*GVHD---------------- 550
FTTMAGR LRDAV K+ S D I G+ +
Sbjct: 538 VPGEFTTMAGRRLRDAV---KMVLTSEEDFEFDDIHIVIAGLTNTYSQYITTYEEYQVQR 594
Query: 549 ----AEIRGSFNSILSVPTFRSW*QPLASGKPVEAGPQQPDLLNKQISLLPPVVVDSTPL 382
+ + G + F+ + L G+PVE GP PDLL KQISLLPPVV+D+TPL
Sbjct: 595 YEGASTLYGPHTLSAYIQEFKKLAEALIYGEPVEPGPLPPDLLEKQISLLPPVVLDATPL 654
Query: 381 GVK-------VPTSPV*LHLQDQGDAVTVVFWSACPRNDLSLL*RSCFRGKIRGSRHTIF 223
GV VP + GD VT FWSACPRNDL ++ I
Sbjct: 655 GVNFGDVCADVPRNST----FKSGDMVTASFWSACPRNDLMTEGTFALVEFLQEKDAWIP 710
Query: 222 AFVSSGRGLQNSALRVMLP*NGKSRNRLCLEFT--------------EGASKSLFRSISH 85
A+ L+ R P SR++ LE+ GA+K LF SI H
Sbjct: 711 AYDDDDFCLRYKWSR---PSKLSSRSKGTLEWMIPQGVTPGVYRLSHFGAAKGLFGSIHH 767
Query: 84 FTGSSNVFVV 55
FTGSS FVV
Sbjct: 768 FTGSSTAFVV 777
Score = 66.6 bits (161), Expect(4) = 7e-40
Identities = 30/51 (58%), Positives = 40/51 (78%)
Frame = -2
Query: 947 ERQFKKAAELFNGASDKLNGKIDFRHTYLDFSQLEVALAKQGGGSETVKSC 795
ERQF+KA +LFN A +++ G +DFRH Y+DFSQLEV ++ Q G SE VK+C
Sbjct: 401 ERQFRKAVDLFNAADEEIEGDVDFRHAYIDFSQLEVTISDQ-GYSEVVKTC 450
Score = 23.9 bits (50), Expect(4) = 7e-40
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -3
Query: 820 EGVRLLKVAMGFAFAAG 770
E V+ AMGFAFAAG
Sbjct: 445 EVVKTCPAAMGFAFAAG 461
Score = 21.2 bits (43), Expect(4) = 7e-40
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = -1
Query: 975 DEFESTCIFGE 943
DEFEST I GE
Sbjct: 391 DEFESTRIIGE 401
>ref|XP_003519651.1| PREDICTED: neutral ceramidase-like [Glycine max]
Length = 768
Score = 115 bits (289), Expect(4) = 7e-40
Identities = 106/307 (34%), Positives = 133/307 (43%), Gaps = 68/307 (22%)
Frame = -1
Query: 771 GNAFWKLVRNVLKTPSKEQTDRQQPMPMLV*LGE-------------------------- 670
GN FWKLVRN+LKTP KEQ D P P+L+ GE
Sbjct: 469 GNPFWKLVRNLLKTPGKEQIDCHHPKPILLDTGEMKLPYDWAPSILPIQVLRVGQLVILS 528
Query: 669 ----FTTMAGRCLRDAVFKEKVCRNSRSD*YLLPIC-------------Y*R*GVHDAE- 544
FTTMAGR LRDAV K + N + + Y V E
Sbjct: 529 VPGEFTTMAGRRLRDAV-KTVLSGNKGFGSNIHVVIAGLTNTYSQYVTTYEEYQVQRYEG 587
Query: 543 ---IRGSFNSILSVPTFRSW*QPLASGKPVEAGPQQPDLLNKQISLLPPVVVDSTPLGVK 373
+ G + F L SG+PVE GPQ PDLL+KQISLL PVV+D+TP+GVK
Sbjct: 588 ASTLYGPHTLSAYIQEFTKLAHALISGQPVEPGPQPPDLLDKQISLLTPVVMDATPIGVK 647
Query: 372 -------VPTSPV*LHLQDQGDAVTVVFWSACPRNDLSLL*RSCFRGKIRGSRHTIFAFV 214
VP + + D V+V FWSACPRNDL ++G + A+
Sbjct: 648 FGDCSSDVPKNST----FKRADMVSVTFWSACPRNDLMTEGTFSLVEFLQGKDMWVPAYD 703
Query: 213 SSGRGLQNSALRVMLP*NGKSRNRLCLEFT--------------EGASKSLFRSISHFTG 76
L+ R P S ++ +E+ GA+K L SI HFTG
Sbjct: 704 DDDFCLRFKWSR---PFKLSSHSKATIEWRIPKDVTPGVYRIKHFGAAKGLLGSIHHFTG 760
Query: 75 SSNVFVV 55
SS+ FVV
Sbjct: 761 SSSAFVV 767
Score = 71.2 bits (173), Expect(4) = 7e-40
Identities = 33/51 (64%), Positives = 42/51 (82%)
Frame = -2
Query: 947 ERQFKKAAELFNGASDKLNGKIDFRHTYLDFSQLEVALAKQGGGSETVKSC 795
ERQFKKA ELFNGAS+++ GK+DFRH ++DFSQL V L+K G SE +K+C
Sbjct: 392 ERQFKKAVELFNGASEQIKGKVDFRHAFIDFSQLGVNLSKV-GASEVIKTC 441
Score = 23.5 bits (49), Expect(4) = 7e-40
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 820 EGVRLLKVAMGFAFAAG 770
E ++ AMGFAFAAG
Sbjct: 436 EVIKTCPAAMGFAFAAG 452
Score = 21.2 bits (43), Expect(4) = 7e-40
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = -1
Query: 975 DEFESTCIFGE 943
DEFEST I GE
Sbjct: 382 DEFESTRIIGE 392
>ref|XP_002279962.2| PREDICTED: neutral ceramidase-like [Vitis vinifera]
Length = 810
Score = 107 bits (268), Expect(3) = 1e-39
Identities = 96/303 (31%), Positives = 126/303 (41%), Gaps = 64/303 (21%)
Frame = -1
Query: 771 GNAFWKLVRNVLKTPSKEQTDRQQPMPMLV*LGE-------------------------- 670
GN FW+LVRNVLKTP K Q D P P+L+ GE
Sbjct: 510 GNPFWRLVRNVLKTPDKVQMDCHHPKPILLDTGEMTKPYDWAPSILPIQILRIGQLVILS 569
Query: 669 ----FTTMAGRCLRDAVFKEKVCRNSRSD*YLLPICY*R*GVHD---------------- 550
FTTMAGR LRDA+ + S+ + + +
Sbjct: 570 VPGEFTTMAGRRLRDALKTALISGGSKEFKNVHVVIAGLTNTYSQYVTTFEEYQVQRYEG 629
Query: 549 -AEIRGSFNSILSVPTFRSW*QPLASGKPVEAGPQQPDLLNKQISLLPPVVVDSTPLGVK 373
+ + G + F+ L + +E G Q PDLL++QISLLPPVV+D TP GVK
Sbjct: 630 ASTLYGPHTLSAYIQEFKKLATALVTSSTIEPGLQPPDLLDQQISLLPPVVLDGTPPGVK 689
Query: 372 VPTSPV*LHLQD---QGDAVTVVFWSACPRNDLSLL*RSCFRGKIRGSRHTIFAFVSSGR 202
+ + +G V V FWSACPRNDL + G + A+
Sbjct: 690 FGDLQFDVPMNSTFKRGGMVNVTFWSACPRNDLMTEGTFALVEILHGKDSWVPAYDDDDF 749
Query: 201 GLQNSALRVMLP*NGKSRNRLCLEFT--------------EGASKSLFRSISHFTGSSNV 64
L+ R P R+ +E+ GASKSLF SISHFTG+S+
Sbjct: 750 CLRFKWSR---PAKLSPRSYATIEWRIPESAAAGVYRIRHFGASKSLFGSISHFTGTSSA 806
Query: 63 FVV 55
FVV
Sbjct: 807 FVV 809
Score = 79.3 bits (194), Expect(3) = 1e-39
Identities = 35/51 (68%), Positives = 44/51 (86%)
Frame = -2
Query: 947 ERQFKKAAELFNGASDKLNGKIDFRHTYLDFSQLEVALAKQGGGSETVKSC 795
+RQF+KA +LFN A+++L GKID+RHTYLDFS+L V L KQGGGSE VK+C
Sbjct: 432 DRQFRKAVDLFNKATEQLKGKIDYRHTYLDFSKLSVTLPKQGGGSEVVKTC 482
Score = 23.9 bits (50), Expect(3) = 1e-39
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -3
Query: 820 EGVRLLKVAMGFAFAAG 770
E V+ AMGFAFAAG
Sbjct: 477 EVVKTCPAAMGFAFAAG 493
>ref|XP_003617915.1| Neutral ceramidase [Medicago truncatula] gi|355519250|gb|AET00874.1|
Neutral ceramidase [Medicago truncatula]
Length = 792
Score = 114 bits (284), Expect(4) = 1e-38
Identities = 103/306 (33%), Positives = 131/306 (42%), Gaps = 67/306 (21%)
Frame = -1
Query: 771 GNAFWKLVRNVLKTPSKEQTDRQQPMPMLV*LGE-------------------------- 670
GN FWKLVRN+LKTP KEQ QQP P+L+ GE
Sbjct: 492 GNPFWKLVRNLLKTPDKEQIACQQPKPILLDTGEMKLPYDWAPTILPIQILRIGQFFILS 551
Query: 669 ----FTTMAGRCLRDAVFKEKVCRNSRSD*YLLPIC------------Y*R*GVHDAE-- 544
FTTMAGR LRDAV S + I Y V E
Sbjct: 552 VPGEFTTMAGRRLRDAVKTVLSGDKSLGSNIHVVIAGLTNTYSQYVTTYEEYEVQRYEGA 611
Query: 543 --IRGSFNSILSVPTFRSW*QPLASGKPVEAGPQQPDLLNKQISLLPPVVVDSTPLGVK- 373
+ G + F+ L +G+PVE+GPQ PDLL+KQI LL PVV+D TPLG
Sbjct: 612 STLYGPHTLDAYIQEFKKLAHALINGQPVESGPQPPDLLDKQIGLLTPVVMDGTPLGTSF 671
Query: 372 ------VPTSPV*LHLQDQGDAVTVVFWSACPRNDLSLL*RSCFRGKIRGSRHTIFAFVS 211
VP + +GD V+V FWSACPRNDL ++G + A+
Sbjct: 672 GDCSSDVPKNST----FKRGDTVSVTFWSACPRNDLMTEGTFSLVEYLQGKDTWVPAYDD 727
Query: 210 SGRGLQNSALRVMLP*NGKSRNRLCLEFT--------------EGASKSLFRSISHFTGS 73
++ R P + ++ +E+ GA+K L SI HFTGS
Sbjct: 728 DDFCVRFKWSR---PFKLSTHSKAAIEWRIPQDVAPGVYRIKHFGAAKGLLGSIRHFTGS 784
Query: 72 SNVFVV 55
S+ FVV
Sbjct: 785 SSAFVV 790
Score = 71.2 bits (173), Expect(4) = 1e-38
Identities = 33/51 (64%), Positives = 42/51 (82%)
Frame = -2
Query: 947 ERQFKKAAELFNGASDKLNGKIDFRHTYLDFSQLEVALAKQGGGSETVKSC 795
ERQFKKA ELFNGAS+++ GK+DFRH YLDFS+LEV ++ G S+ VK+C
Sbjct: 415 ERQFKKAVELFNGASEQIKGKVDFRHAYLDFSKLEVNVS-SNGASKVVKTC 464
Score = 21.6 bits (44), Expect(4) = 1e-38
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -3
Query: 814 VRLLKVAMGFAFAAG 770
V+ AMGF FAAG
Sbjct: 461 VKTCPAAMGFGFAAG 475
Score = 21.2 bits (43), Expect(4) = 1e-38
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = -1
Query: 975 DEFESTCIFGE 943
DEFEST I GE
Sbjct: 405 DEFESTRIIGE 415