BLASTX nr result
ID: Atractylodes21_contig00004769
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes21_contig00004769
(2516 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN71863.1| hypothetical protein VITISV_023530 [Vitis vinifera] 1129 0.0
ref|XP_002263291.2| PREDICTED: leucine-rich repeat receptor-like... 1126 0.0
ref|XP_002526451.1| Receptor protein kinase CLAVATA1 precursor, ... 1119 0.0
ref|XP_002328778.1| predicted protein [Populus trichocarpa] gi|2... 1105 0.0
ref|XP_003592924.1| Receptor-like protein kinase [Medicago trunc... 1092 0.0
>emb|CAN71863.1| hypothetical protein VITISV_023530 [Vitis vinifera]
Length = 954
Score = 1129 bits (2920), Expect = 0.0
Identities = 564/752 (75%), Positives = 638/752 (84%)
Frame = -3
Query: 2514 GIPKEFGKLVNLVHMDLSSCGLDGPIPPELGNLHSVDTIFLHINLLSGPIPRQLGNLTSL 2335
GIP EFGKL+NLVHMDLSSC +DG IP ELGNL S++T+FLHIN LSG IP +LGNLTSL
Sbjct: 203 GIPSEFGKLINLVHMDLSSCEJDGHIPEELGNLKSLNTLFLHINQLSGSIPNRLGNLTSL 262
Query: 2334 VYLDLSANALSGEIPXXXXXXXXXXXXXXXXNRLHGSIPDFVADYPDLQTLGLWSNNFTG 2155
V LDLS NAL+GEIP NRLHGSIPDFVA+ P+LQTLGLW NNFTG
Sbjct: 263 VNLDLSNNALTGEIPLELSNLLQLSLLNLFLNRLHGSIPDFVAELPNLQTLGLWMNNFTG 322
Query: 2154 VIPENLGRNQNLRELDLSTNKLTGTIPRDLCASNQLRVLILLKNFLFGSIPEDLGTCSSL 1975
+IPE LG+N L+ELDLS+NKLTG IP +LC+SNQLR+LILLKNFLFG IPE LG CSSL
Sbjct: 323 IIPERLGQNGRLQELDLSSNKLTGAIPGNLCSSNQLRILILLKNFLFGPIPEGLGRCSSL 382
Query: 1974 VRVRLGENYLNGSIPDGXXXXXXXXXXXLHNNYLSGNLSDNRDPSSRPVKXXXXXXXXXX 1795
RVRLG+NYLNGSIP G L NNY+SG L +N + SS P K
Sbjct: 383 TRVRLGQNYLNGSIPGGFIYLPLLNLMELQNNYISGTLPENHNSSSIPEKLGELNLSNNL 442
Query: 1794 XSGYLPFSLSNFSSLQILLLAGNRFSGPIPTTIGQLHQVSKLDLSGNSLSGEIPPDIGNC 1615
SG LP SLSNF+SLQILLL GN+FSGPIP +IG+L QV KLDLS NSLSGEIP +IG C
Sbjct: 443 LSGRLPSSLSNFTSLQILLLGGNQFSGPIPPSIGELKQVLKLDLSRNSLSGEIPLEIGAC 502
Query: 1614 VHLTYLDLSQNNLSGSIPLEVSNIRILNYLNVSRNHLNDSIPKSIETMKSLTIADFSFND 1435
HLTYLD+SQNNLSG IP EVSNI+I+NYLN+SRNHL+++IPKSI +MKSLTIADFSFN+
Sbjct: 503 FHLTYLDISQNNLSGPIPSEVSNIKIMNYLNLSRNHLSEAIPKSIGSMKSLTIADFSFNE 562
Query: 1434 LSGKLPESGQFTFFNASSFAGNPQLCGQLLNNPCNVSGISDSVNKKPGRFKLIFALGLLI 1255
LSGKLPESGQF FFNASS+AGNP LCG LLNNPCN + I+ + K P FKLIFALGLLI
Sbjct: 563 LSGKLPESGQFAFFNASSYAGNPHLCGSLLNNPCNFTAINGTPGKPPADFKLIFALGLLI 622
Query: 1254 CSLVFAAAAMVKAKSYRRSGSDVWKMTSFQKLEFTVADVLECIKDGNVIGRGGAGIVYHG 1075
CSLVFAAAA++KAKS++++ SD W+MT+FQK+EFTVADVLEC+KDGNVIGRGGAGIVYHG
Sbjct: 623 CSLVFAAAAIIKAKSFKKTASDSWRMTAFQKVEFTVADVLECVKDGNVIGRGGAGIVYHG 682
Query: 1074 KMPNGTEIAVKKLVGFGNTSHDHGFRAEIRTLGNIRHRNIVRLLAFCSNKDTNLLVYEYM 895
KMP G E+AVKKL+GFG SHDHGFRAEI+TLGNIRHRNIVRL+AFCSNK+TNLLVYEYM
Sbjct: 683 KMPTGAEVAVKKLLGFGPNSHDHGFRAEIQTLGNIRHRNIVRLIAFCSNKETNLLVYEYM 742
Query: 894 RNGSLGEALHAKIKGGILGWHLRYKISIEAARGLCYLHHDCSPLIVHRDVKSNNILLNSS 715
+NGSLGEALH K KGG LGW+LRYKI+++AA+GLCYLHHDCSPLIVHRDVKSNNILLNSS
Sbjct: 743 KNGSLGEALHGK-KGGFLGWNLRYKIAVDAAKGLCYLHHDCSPLIVHRDVKSNNILLNSS 801
Query: 714 YEAHVADFGLAKYLVDGGASECMSAIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELI 535
+EAHVADFGLAK+L+DGGASECMSAIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELI
Sbjct: 802 FEAHVADFGLAKFLIDGGASECMSAIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELI 861
Query: 534 TGKRPVGGFGDGVDIVQWARTSTRCRKEEVNCIIDSRLVNVPQEEAMHLFFIAMLCIQEN 355
TG+RPVG FG+GVDIVQWA+ +T C KE V I+D RL +P+ EA HLFFIA+LCI+EN
Sbjct: 862 TGRRPVGDFGEGVDIVQWAKRTTNCCKENVIXIVDPRLATIPRNEATHLFFIALLCIEEN 921
Query: 354 SVERPTMREVVQMLSEFPRDSPENQTSSSSIV 259
SVERPTMREVVQMLSE R+SP+N+TSSSSIV
Sbjct: 922 SVERPTMREVVQMLSESHRNSPDNKTSSSSIV 953
Score = 137 bits (344), Expect = 2e-29
Identities = 111/366 (30%), Positives = 163/366 (44%)
Frame = -3
Query: 2514 GIPKEFGKLVNLVHMDLSSCGLDGPIPPELGNLHSVDTIFLHINLLSGPIPRQLGNLTSL 2335
GI G++V L DL+ L G + P++ L + I + N +GPI ++ NL+SL
Sbjct: 39 GIQCAHGRVVGL---DLTDMNLCGSVSPDISRLDQLSNISISGNNFTGPI--EIQNLSSL 93
Query: 2334 VYLDLSANALSGEIPXXXXXXXXXXXXXXXXNRLHGSIPDFVADYPDLQTLGLWSNNFTG 2155
+L++S N SG + + DL+ L ++NNFT
Sbjct: 94 RWLNISNNQFSGSLNWSF------------------------STMEDLEVLDAYNNNFTA 129
Query: 2154 VIPENLGRNQNLRELDLSTNKLTGTIPRDLCASNQLRVLILLKNFLFGSIPEDLGTCSSL 1975
++P+ + + LR LDL N G IP+ L L L N L G IP +LG +SL
Sbjct: 130 LLPQGVLSLKKLRYLDLGGNFFYGKIPKIYGGLAALEYLSLAGNDLRGKIPIELGNLTSL 189
Query: 1974 VRVRLGENYLNGSIPDGXXXXXXXXXXXLHNNYLSGNLSDNRDPSSRPVKXXXXXXXXXX 1795
+ LG Y N S DG +H + S +
Sbjct: 190 KEIYLG--YYN-SFTDGIPSEFGKLINLVHMDLSSCEJD--------------------- 225
Query: 1794 XSGYLPFSLSNFSSLQILLLAGNRFSGPIPTTIGQLHQVSKLDLSGNSLSGEIPPDIGNC 1615
G++P L N SL L L N+ SG IP +G L + LDLS N+L+GEIP ++ N
Sbjct: 226 --GHIPEELGNLKSLNTLFLHINQLSGSIPNRLGNLTSLVNLDLSNNALTGEIPLELSNL 283
Query: 1614 VHLTYLDLSQNNLSGSIPLEVSNIRILNYLNVSRNHLNDSIPKSIETMKSLTIADFSFND 1435
+ L+ L+L N L GSIP V+ + L L + N+ IP+ + L D S N
Sbjct: 284 LQLSLLNLFLNRLHGSIPDFVAELPNLQTLGLWMNNFTGIIPERLGQNGRLQELDLSSNK 343
Query: 1434 LSGKLP 1417
L+G +P
Sbjct: 344 LTGAIP 349
Score = 97.8 bits (242), Expect = 1e-17
Identities = 77/275 (28%), Positives = 120/275 (43%), Gaps = 1/275 (0%)
Frame = -3
Query: 2235 LHGSIPDFVADYPDLQTLGLWSNNFTGVIPENLGRNQNLRELDLSTNKLTGTIPRDLCAS 2056
L GS+ ++ L + + NNFTG P + +LR L++S N+ +G++
Sbjct: 57 LCGSVSPDISRLDQLSNISISGNNFTG--PIEIQNLSSLRWLNISNNQFSGSLNWSFSTM 114
Query: 2055 NQLRVLILLKNFLFGSIPEDLGTCSSLVRVRLGENYLNGSIPDGXXXXXXXXXXXLHNNY 1876
L VL N +P+ + + L + LG N+ G IP Y
Sbjct: 115 EDLEVLDAYNNNFTALLPQGVLSLKKLRYLDLGGNFFYGKIPKIYGGLAAL-------EY 167
Query: 1875 LSGNLSDNRDPSSRPVKXXXXXXXXXXXSGYLPFSLSNFSSLQILLLAG-NRFSGPIPTT 1699
LS +D R G +P L N +SL+ + L N F+ IP+
Sbjct: 168 LSLAGNDLR--------------------GKIPIELGNLTSLKEIYLGYYNSFTDGIPSE 207
Query: 1698 IGQLHQVSKLDLSGNSLSGEIPPDIGNCVHLTYLDLSQNNLSGSIPLEVSNIRILNYLNV 1519
G+L + +DLS + G IP ++GN L L L N LSGSIP + N+ L L++
Sbjct: 208 FGKLINLVHMDLSSCEJDGHIPEELGNLKSLNTLFLHINQLSGSIPNRLGNLTSLVNLDL 267
Query: 1518 SRNHLNDSIPKSIETMKSLTIADFSFNDLSGKLPE 1414
S N L IP + + L++ + N L G +P+
Sbjct: 268 SNNALTGEIPLELSNLLQLSLLNLFLNRLHGSIPD 302
>ref|XP_002263291.2| PREDICTED: leucine-rich repeat receptor-like serine/threonine-protein
kinase BAM1-like [Vitis vinifera]
Length = 976
Score = 1126 bits (2913), Expect = 0.0
Identities = 564/752 (75%), Positives = 637/752 (84%)
Frame = -3
Query: 2514 GIPKEFGKLVNLVHMDLSSCGLDGPIPPELGNLHSVDTIFLHINLLSGPIPRQLGNLTSL 2335
GIP EFGKL+NLVHMDLSSC LDG IP ELGNL S++T+FLHIN LSG IP +LGNLTSL
Sbjct: 225 GIPSEFGKLINLVHMDLSSCELDGHIPEELGNLKSLNTLFLHINQLSGSIPNRLGNLTSL 284
Query: 2334 VYLDLSANALSGEIPXXXXXXXXXXXXXXXXNRLHGSIPDFVADYPDLQTLGLWSNNFTG 2155
V LDLS NAL+GEIP NRLHGSIPDFVA+ P+LQTLGLW NNFTG
Sbjct: 285 VNLDLSNNALTGEIPLELSNLLQLSLLNLFLNRLHGSIPDFVAELPNLQTLGLWMNNFTG 344
Query: 2154 VIPENLGRNQNLRELDLSTNKLTGTIPRDLCASNQLRVLILLKNFLFGSIPEDLGTCSSL 1975
+IPE LG+N L+ELDLS+NKLTG IP +LC+SNQLR+LILLKNFLFG IPE LG CSSL
Sbjct: 345 IIPERLGQNGRLQELDLSSNKLTGAIPGNLCSSNQLRILILLKNFLFGPIPEGLGRCSSL 404
Query: 1974 VRVRLGENYLNGSIPDGXXXXXXXXXXXLHNNYLSGNLSDNRDPSSRPVKXXXXXXXXXX 1795
RVRLG+NYLNGSIP G L NNY+SG L +N + S P K
Sbjct: 405 TRVRLGQNYLNGSIPGGFIYLPLLNLMELQNNYISGTLPENHNSSFIPEKLGELNLSNNL 464
Query: 1794 XSGYLPFSLSNFSSLQILLLAGNRFSGPIPTTIGQLHQVSKLDLSGNSLSGEIPPDIGNC 1615
SG LP SLSNF+SLQILLL GN+FSGPIP +IG+L QV KLDLS NSLSGEIP +IG C
Sbjct: 465 LSGRLPSSLSNFTSLQILLLGGNQFSGPIPPSIGELKQVLKLDLSRNSLSGEIPLEIGAC 524
Query: 1614 VHLTYLDLSQNNLSGSIPLEVSNIRILNYLNVSRNHLNDSIPKSIETMKSLTIADFSFND 1435
HLTYLD+SQNNLSG IP EVSNI+I+NYLN+SRNHL+++IPKSI +MKSLTIADFSFN+
Sbjct: 525 FHLTYLDISQNNLSGPIPSEVSNIKIMNYLNLSRNHLSEAIPKSIGSMKSLTIADFSFNE 584
Query: 1434 LSGKLPESGQFTFFNASSFAGNPQLCGQLLNNPCNVSGISDSVNKKPGRFKLIFALGLLI 1255
LSGKLPESGQF FFNASS+AGNP LCG LLNNPCN + I+ + K P FKLIFALGLLI
Sbjct: 585 LSGKLPESGQFAFFNASSYAGNPHLCGSLLNNPCNFTAINGTPGKPPADFKLIFALGLLI 644
Query: 1254 CSLVFAAAAMVKAKSYRRSGSDVWKMTSFQKLEFTVADVLECIKDGNVIGRGGAGIVYHG 1075
CSLVFAAAA++KAKS++++ SD W+MT+FQK+EFTVADVLEC+KDGNVIGRGGAGIVYHG
Sbjct: 645 CSLVFAAAAIIKAKSFKKTASDSWRMTAFQKVEFTVADVLECVKDGNVIGRGGAGIVYHG 704
Query: 1074 KMPNGTEIAVKKLVGFGNTSHDHGFRAEIRTLGNIRHRNIVRLLAFCSNKDTNLLVYEYM 895
KMP G E+AVKKL+GFG SHDHGFRAEI+TLGNIRHRNIVRL+AFCSNK+TNLLVYEYM
Sbjct: 705 KMPTGAEVAVKKLLGFGPNSHDHGFRAEIQTLGNIRHRNIVRLIAFCSNKETNLLVYEYM 764
Query: 894 RNGSLGEALHAKIKGGILGWHLRYKISIEAARGLCYLHHDCSPLIVHRDVKSNNILLNSS 715
+NGSLGEALH K KGG LGW+LRYKI+++AA+GLCYLHHDCSPLIVHRDVKSNNILLNSS
Sbjct: 765 KNGSLGEALHGK-KGGFLGWNLRYKIAVDAAKGLCYLHHDCSPLIVHRDVKSNNILLNSS 823
Query: 714 YEAHVADFGLAKYLVDGGASECMSAIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELI 535
+EAHVADFGLAK+L+DGGASECMSAIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELI
Sbjct: 824 FEAHVADFGLAKFLIDGGASECMSAIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELI 883
Query: 534 TGKRPVGGFGDGVDIVQWARTSTRCRKEEVNCIIDSRLVNVPQEEAMHLFFIAMLCIQEN 355
TG+RPVG FG+GVDIVQWA+ +T C KE V I+D RL +P+ EA HLFFIA+LCI+EN
Sbjct: 884 TGRRPVGDFGEGVDIVQWAKRTTNCCKENVIRIVDPRLATIPRNEATHLFFIALLCIEEN 943
Query: 354 SVERPTMREVVQMLSEFPRDSPENQTSSSSIV 259
SVERPTMREVVQMLSE R+SP+N+TSSSSIV
Sbjct: 944 SVERPTMREVVQMLSESHRNSPDNKTSSSSIV 975
Score = 137 bits (345), Expect = 1e-29
Identities = 112/366 (30%), Positives = 163/366 (44%)
Frame = -3
Query: 2514 GIPKEFGKLVNLVHMDLSSCGLDGPIPPELGNLHSVDTIFLHINLLSGPIPRQLGNLTSL 2335
GI G++V L DL+ L G + P++ L + I + N +GPI ++ NL+SL
Sbjct: 61 GIQCAHGRVVGL---DLTDMNLCGSVSPDISRLDQLSNISISGNNFTGPI--EIQNLSSL 115
Query: 2334 VYLDLSANALSGEIPXXXXXXXXXXXXXXXXNRLHGSIPDFVADYPDLQTLGLWSNNFTG 2155
+L++S N SG + + DL+ L ++NNFT
Sbjct: 116 RWLNISNNQFSGSLNWSF------------------------STMEDLEVLDAYNNNFTA 151
Query: 2154 VIPENLGRNQNLRELDLSTNKLTGTIPRDLCASNQLRVLILLKNFLFGSIPEDLGTCSSL 1975
++P+ + + LR LDL N G IP+ L L L N L G IP +LG +SL
Sbjct: 152 LLPQGVLSLKKLRYLDLGGNFFYGKIPKIYGGLAALEYLSLAGNDLRGKIPIELGNLTSL 211
Query: 1974 VRVRLGENYLNGSIPDGXXXXXXXXXXXLHNNYLSGNLSDNRDPSSRPVKXXXXXXXXXX 1795
+ LG Y N S DG +H + S L
Sbjct: 212 KEIYLG--YYN-SFTDGIPSEFGKLINLVHMDLSSCELD--------------------- 247
Query: 1794 XSGYLPFSLSNFSSLQILLLAGNRFSGPIPTTIGQLHQVSKLDLSGNSLSGEIPPDIGNC 1615
G++P L N SL L L N+ SG IP +G L + LDLS N+L+GEIP ++ N
Sbjct: 248 --GHIPEELGNLKSLNTLFLHINQLSGSIPNRLGNLTSLVNLDLSNNALTGEIPLELSNL 305
Query: 1614 VHLTYLDLSQNNLSGSIPLEVSNIRILNYLNVSRNHLNDSIPKSIETMKSLTIADFSFND 1435
+ L+ L+L N L GSIP V+ + L L + N+ IP+ + L D S N
Sbjct: 306 LQLSLLNLFLNRLHGSIPDFVAELPNLQTLGLWMNNFTGIIPERLGQNGRLQELDLSSNK 365
Query: 1434 LSGKLP 1417
L+G +P
Sbjct: 366 LTGAIP 371
Score = 98.2 bits (243), Expect = 9e-18
Identities = 78/275 (28%), Positives = 120/275 (43%), Gaps = 1/275 (0%)
Frame = -3
Query: 2235 LHGSIPDFVADYPDLQTLGLWSNNFTGVIPENLGRNQNLRELDLSTNKLTGTIPRDLCAS 2056
L GS+ ++ L + + NNFTG P + +LR L++S N+ +G++
Sbjct: 79 LCGSVSPDISRLDQLSNISISGNNFTG--PIEIQNLSSLRWLNISNNQFSGSLNWSFSTM 136
Query: 2055 NQLRVLILLKNFLFGSIPEDLGTCSSLVRVRLGENYLNGSIPDGXXXXXXXXXXXLHNNY 1876
L VL N +P+ + + L + LG N+ G IP Y
Sbjct: 137 EDLEVLDAYNNNFTALLPQGVLSLKKLRYLDLGGNFFYGKIPKIYGGLAAL-------EY 189
Query: 1875 LSGNLSDNRDPSSRPVKXXXXXXXXXXXSGYLPFSLSNFSSLQILLLAG-NRFSGPIPTT 1699
LS +D R G +P L N +SL+ + L N F+ IP+
Sbjct: 190 LSLAGNDLR--------------------GKIPIELGNLTSLKEIYLGYYNSFTDGIPSE 229
Query: 1698 IGQLHQVSKLDLSGNSLSGEIPPDIGNCVHLTYLDLSQNNLSGSIPLEVSNIRILNYLNV 1519
G+L + +DLS L G IP ++GN L L L N LSGSIP + N+ L L++
Sbjct: 230 FGKLINLVHMDLSSCELDGHIPEELGNLKSLNTLFLHINQLSGSIPNRLGNLTSLVNLDL 289
Query: 1518 SRNHLNDSIPKSIETMKSLTIADFSFNDLSGKLPE 1414
S N L IP + + L++ + N L G +P+
Sbjct: 290 SNNALTGEIPLELSNLLQLSLLNLFLNRLHGSIPD 324
>ref|XP_002526451.1| Receptor protein kinase CLAVATA1 precursor, putative [Ricinus
communis] gi|223534231|gb|EEF35946.1| Receptor protein
kinase CLAVATA1 precursor, putative [Ricinus communis]
Length = 996
Score = 1119 bits (2895), Expect = 0.0
Identities = 561/774 (72%), Positives = 643/774 (83%)
Frame = -3
Query: 2514 GIPKEFGKLVNLVHMDLSSCGLDGPIPPELGNLHSVDTIFLHINLLSGPIPRQLGNLTSL 2335
GIP EFG L+NLV MDLSSCGLDGPIP ELGNL +DT+ L+IN LSG IP++LGNLT+L
Sbjct: 224 GIPAEFGSLMNLVQMDLSSCGLDGPIPRELGNLKMLDTLHLYINHLSGSIPKELGNLTNL 283
Query: 2334 VYLDLSANALSGEIPXXXXXXXXXXXXXXXXNRLHGSIPDFVADYPDLQTLGLWSNNFTG 2155
LDLS NAL+GEIP NRLHGSIPD+VAD P+L+TL LW NNFTG
Sbjct: 284 ANLDLSYNALTGEIPFEFISLKQLKLFNLFMNRLHGSIPDYVADLPNLETLELWMNNFTG 343
Query: 2154 VIPENLGRNQNLRELDLSTNKLTGTIPRDLCASNQLRVLILLKNFLFGSIPEDLGTCSSL 1975
IP LG+N L+ LDLS+NKLTGTIP+ LC+SNQL++LIL+KNFLFG IP+ LG C SL
Sbjct: 344 EIPRKLGQNGKLQALDLSSNKLTGTIPQGLCSSNQLKILILMKNFLFGPIPDGLGRCYSL 403
Query: 1974 VRVRLGENYLNGSIPDGXXXXXXXXXXXLHNNYLSGNLSDNRDPSSRPVKXXXXXXXXXX 1795
R+RLG+NYLNGSIPDG L NN LSG LS+N + SSRPV+
Sbjct: 404 TRLRLGQNYLNGSIPDGLIYLPELNLAELQNNVLSGTLSENCNSSSRPVRLGQLNLSNNL 463
Query: 1794 XSGYLPFSLSNFSSLQILLLAGNRFSGPIPTTIGQLHQVSKLDLSGNSLSGEIPPDIGNC 1615
SG LPFS+SNFSSLQILLL+GN+FSGPIP +IG L QV KLD+S NSLSG IPP+IG+C
Sbjct: 464 LSGPLPFSISNFSSLQILLLSGNQFSGPIPPSIGVLRQVLKLDVSRNSLSGSIPPEIGSC 523
Query: 1614 VHLTYLDLSQNNLSGSIPLEVSNIRILNYLNVSRNHLNDSIPKSIETMKSLTIADFSFND 1435
HLT+LD+SQNNLSG IP E+S+I ILNYLN+SRNHLN +IPKSI +MKSLTIADFSFND
Sbjct: 524 FHLTFLDMSQNNLSGLIPPEISDIHILNYLNLSRNHLNQTIPKSIGSMKSLTIADFSFND 583
Query: 1434 LSGKLPESGQFTFFNASSFAGNPQLCGQLLNNPCNVSGISDSVNKKPGRFKLIFALGLLI 1255
SGKLPESGQF+FFNASSFAGNPQLCG LLNNPCN + I+++ K P FKLIFALGLLI
Sbjct: 584 FSGKLPESGQFSFFNASSFAGNPQLCGPLLNNPCNFTAITNTPGKAPNDFKLIFALGLLI 643
Query: 1254 CSLVFAAAAMVKAKSYRRSGSDVWKMTSFQKLEFTVADVLECIKDGNVIGRGGAGIVYHG 1075
CSL+FA AA++KAKS +++ SD WK+T+FQK+EFTV D+LEC+KDGNVIGRGGAGIVYHG
Sbjct: 644 CSLIFAIAAIIKAKSSKKNSSDSWKLTAFQKIEFTVTDILECVKDGNVIGRGGAGIVYHG 703
Query: 1074 KMPNGTEIAVKKLVGFGNTSHDHGFRAEIRTLGNIRHRNIVRLLAFCSNKDTNLLVYEYM 895
KMPNG E+AVKKL+GFG SHDHGFRAEI+TLGNIRHRNIVRLLAFCSNK+TNLLVYEYM
Sbjct: 704 KMPNGVEVAVKKLLGFGTHSHDHGFRAEIQTLGNIRHRNIVRLLAFCSNKETNLLVYEYM 763
Query: 894 RNGSLGEALHAKIKGGILGWHLRYKISIEAARGLCYLHHDCSPLIVHRDVKSNNILLNSS 715
RNGSLGEALH K KG L W+LRYKI+IEAA+GLCYLHHDCSPLIVHRDVKSNNILLNSS
Sbjct: 764 RNGSLGEALHGK-KGAFLSWNLRYKIAIEAAKGLCYLHHDCSPLIVHRDVKSNNILLNSS 822
Query: 714 YEAHVADFGLAKYLVDGGASECMSAIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELI 535
+EAHVADFGLAK+L+DGGASECMSAIAGSYGYIAPEYAYTL+VDEKSDVYSFGVVLLEL+
Sbjct: 823 FEAHVADFGLAKFLIDGGASECMSAIAGSYGYIAPEYAYTLKVDEKSDVYSFGVVLLELL 882
Query: 534 TGKRPVGGFGDGVDIVQWARTSTRCRKEEVNCIIDSRLVNVPQEEAMHLFFIAMLCIQEN 355
TG+RPVG FGDGVDIVQW++ T RKE+V IIDSRL VP++E MHLFFIA+LC QEN
Sbjct: 883 TGRRPVGDFGDGVDIVQWSKRVTNNRKEDVLNIIDSRLTMVPKDEVMHLFFIALLCSQEN 942
Query: 354 SVERPTMREVVQMLSEFPRDSPENQTSSSSIVFLQPKKLDKDRTYPKLGQDLLV 193
S+ERPTMREVVQMLSEF R S + +SSSS++F Q K + ++ Y K DLLV
Sbjct: 943 SIERPTMREVVQMLSEFHRHSLDYHSSSSSVIFQQLKNSETEKKYAKFKSDLLV 996
Score = 110 bits (276), Expect = 1e-21
Identities = 93/351 (26%), Positives = 141/351 (40%), Gaps = 1/351 (0%)
Frame = -3
Query: 2379 LSGPIPRQLGNLTSLVYLDLSANALSGEIPXXXXXXXXXXXXXXXXNRLHGSIPDFVADY 2200
L G + QL L LV L L+ N +G + +
Sbjct: 78 LYGSVSPQLSRLDRLVNLSLAGNNFTGTVE--------------------------IIRL 111
Query: 2199 PDLQTLGLWSNNFTGVIPENLGRNQNLRELDLSTNKLTGTIPRDLCASNQLRVLILLKNF 2020
L+ L + +N F+G + N NL D N T +P + + +LR L L NF
Sbjct: 112 SSLRFLNISNNQFSGGLDWNYSEMANLEVFDAYNNNFTAFLPLGILSLKKLRYLDLGGNF 171
Query: 2019 LFGSIPEDLGTCSSLVRVRLGENYLNGSIPDGXXXXXXXXXXXLHNNYLSGNLSDNRDPS 1840
+G+IP G L + L N L G IP GNLS+ ++
Sbjct: 172 FYGNIPPSYGRLVGLEYLSLAGNDLRGRIPGEL-----------------GNLSNLKE-- 212
Query: 1839 SRPVKXXXXXXXXXXXSGYLPFSLSNFSSLQILLLAGNRFSGPIPTTIGQLHQVSKLDLS 1660
G +P + +L + L+ GPIP +G L + L L
Sbjct: 213 -------IFLGHYNVFEGGIPAEFGSLMNLVQMDLSSCGLDGPIPRELGNLKMLDTLHLY 265
Query: 1659 GNSLSGEIPPDIGNCVHLTYLDLSQNNLSGSIPLEVSNIRILNYLNVSRNHLNDSIPKSI 1480
N LSG IP ++GN +L LDLS N L+G IP E +++ L N+ N L+ SIP +
Sbjct: 266 INHLSGSIPKELGNLTNLANLDLSYNALTGEIPFEFISLKQLKLFNLFMNRLHGSIPDYV 325
Query: 1479 ETMKSLTIADFSFNDLSGKLPES-GQFTFFNASSFAGNPQLCGQLLNNPCN 1330
+ +L + N+ +G++P GQ A + N +L G + C+
Sbjct: 326 ADLPNLETLELWMNNFTGEIPRKLGQNGKLQALDLSSN-KLTGTIPQGLCS 375
>ref|XP_002328778.1| predicted protein [Populus trichocarpa] gi|222839076|gb|EEE77427.1|
predicted protein [Populus trichocarpa]
Length = 1001
Score = 1105 bits (2859), Expect = 0.0
Identities = 557/781 (71%), Positives = 637/781 (81%), Gaps = 8/781 (1%)
Frame = -3
Query: 2511 IPKEFGKLVNLVHMDLSSCGLDGPIPPELGNLHSVDTIFLHINLLSGPIPRQLGNLTSLV 2332
IP E LVNLVHMDLSSCGLDGPIP ELGNL + T++LHIN LSG IP++LGNLT+LV
Sbjct: 223 IPVELSNLVNLVHMDLSSCGLDGPIPNELGNLKLLHTLYLHINFLSGSIPKELGNLTNLV 282
Query: 2331 YLDLSANALSGEIPXXXXXXXXXXXXXXXXNRLHGSIPDFVADYPDLQTLGLWSNNFTGV 2152
LDLS NAL+GEIP NRLHGSIPD+VAD P+L+TL LW NNFTG
Sbjct: 283 NLDLSYNALTGEIPFEFINLKQLNLLNLFLNRLHGSIPDYVADLPNLETLQLWKNNFTGE 342
Query: 2151 IPENLGRNQNLRELDLSTNKLTGTIPRDLCASNQLRVLILLKNFLFGSIPEDLGTCSSLV 1972
IP NLGRN L+ LDLS+NKLTGT+P+DLC+SNQLR+LIL KNFLFG IPE LG C SL
Sbjct: 343 IPPNLGRNGKLQLLDLSSNKLTGTVPQDLCSSNQLRILILFKNFLFGPIPEGLGACYSLT 402
Query: 1971 RVRLGENYLNGSIPDGXXXXXXXXXXXLHNNYLSGNLSDNRDPSSRPVKXXXXXXXXXXX 1792
+VRLG+NYLNGSIP G +NYLSG LS+N + S +PVK
Sbjct: 403 KVRLGQNYLNGSIPIGFIYLPELILAEFQSNYLSGTLSENGNSSLKPVKLGQLDLSNNLF 462
Query: 1791 SGYLPFSLSNFSSLQILLLAGNRFSGPIPTTIGQLHQVSKLDLSGNSLSGEIPPDIGNCV 1612
SG LP SLSNFSSLQ LLL+GN+FSGPIP IG+L QV KLDLS NS SG +PP+IGNC
Sbjct: 463 SGPLPSSLSNFSSLQTLLLSGNKFSGPIPPMIGELLQVLKLDLSRNSFSGPVPPEIGNCF 522
Query: 1611 HLTYLDLSQNNLSGSIPLEVSNIRILNYLNVSRNHLNDSIPKSIETMKSLTIADFSFNDL 1432
HLT+LD+SQNNLSG IP ++SNIR LNYLN+SRNHLN +IPKS+ ++KSLT+ADFSFND
Sbjct: 523 HLTFLDMSQNNLSGPIPSDMSNIRNLNYLNLSRNHLNQTIPKSLGSLKSLTVADFSFNDF 582
Query: 1431 SGKLPESGQFTFFNASSFAGNPQLCGQLLNNPCNVSGISDSVNKKPGRFKLIFALGLLIC 1252
+GKLPESGQF+ FNASSFAGNP LCG LLNNPCN + ++++ K P FKLIFALGLLIC
Sbjct: 583 AGKLPESGQFSLFNASSFAGNPLLCGPLLNNPCNFTTVTNTPGKAPSNFKLIFALGLLIC 642
Query: 1251 SLVFAAAAMVKAKSYRRSGSDVWKMTSFQKLEFTVADVLECIKDGNVIGRGGAGIVYHGK 1072
SL+FA AA++KAK++++S SD WK+T+FQKLEFTV D++EC+KDGNVIGRGGAGIVYHGK
Sbjct: 643 SLIFATAALIKAKTFKKSSSDSWKLTTFQKLEFTVTDIIECVKDGNVIGRGGAGIVYHGK 702
Query: 1071 MPNGTEIAVKKLVGFGNTSHDHGFRAEIRTLGNIRHRNIVRLLAFCSNKDTNLLVYEYMR 892
MPNG EIAVKKL+GFGN SHDHGFRAEI+TLGNIRHRNIVRLLAFCSNKDTNLLVYEYMR
Sbjct: 703 MPNGVEIAVKKLLGFGNNSHDHGFRAEIQTLGNIRHRNIVRLLAFCSNKDTNLLVYEYMR 762
Query: 891 NGSLGEALHAKIKGGILGWHLRYKISIEAARGLCYLHHDCSPLIVHRDVKSNNILLNSSY 712
NGSLGEALH K LGW+LRYKI+IEAA+GLCYLHHDCSPLIVHRDVKSNNILLNSS+
Sbjct: 763 NGSLGEALHGKKGALFLGWNLRYKIAIEAAKGLCYLHHDCSPLIVHRDVKSNNILLNSSF 822
Query: 711 EAHVADFGLAKYLVDGGASECMSAIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELIT 532
EAHVADFGLAK+LVDGGAS+CMSAIAGSYGYIAPEYAYTL+VDEKSDVYSFGVVLLEL+T
Sbjct: 823 EAHVADFGLAKFLVDGGASQCMSAIAGSYGYIAPEYAYTLKVDEKSDVYSFGVVLLELLT 882
Query: 531 GKRPVGGFGDGVDIVQWARTSTRCRKEEVNCIIDSRLVNVPQEEAMHLFFIAMLCIQENS 352
G+RPVG FGDGVDIVQW++ +T RKE+ I+D RL VP++EAMHLFFIAMLC QENS
Sbjct: 883 GRRPVGDFGDGVDIVQWSKRATNSRKEDAMHIVDPRLTMVPKDEAMHLFFIAMLCSQENS 942
Query: 351 VERPTMREVVQMLSEFPR-DSPENQTSSSSIVFLQPKKLDKDRTYP-------KLGQDLL 196
+ERPTMREVVQMLSEFPR SP+ SSSS + P+++ K T K QDLL
Sbjct: 943 IERPTMREVVQMLSEFPRHTSPDYYLSSSSSIL--PQQMKKSGTEKKEIVNGLKYKQDLL 1000
Query: 195 V 193
V
Sbjct: 1001 V 1001
Score = 115 bits (288), Expect = 6e-23
Identities = 86/280 (30%), Positives = 125/280 (44%), Gaps = 7/280 (2%)
Frame = -3
Query: 2235 LHGSIPDFVADYPDLQTLGLWSNNFTGVIPENLGRNQNLRELDLSTNKLTGTIPRDLCAS 2056
L+GS+ ++ L +L L NNF+G I L NLR L++S N+ G + + +
Sbjct: 76 LYGSVSPQISKLDQLTSLSLAGNNFSGAI--ELAGMSNLRFLNISNNQFNGGLDWNYTSI 133
Query: 2055 NQLRVLILLKNFLFGSIPEDLGTCSSLVRVRLGENYLNGSIPDGXXXXXXXXXXXLHNNY 1876
L V N +P + L + LG NY G IP L N
Sbjct: 134 ADLEVFDAFDNNFTAFLPLGILNLKKLRHLELGGNYFYGKIPTSYGELAGLEYLSLMGNN 193
Query: 1875 LSG-------NLSDNRDPSSRPVKXXXXXXXXXXXSGYLPFSLSNFSSLQILLLAGNRFS 1717
L G NL++ R+ G +P LSN +L + L+
Sbjct: 194 LQGKIPGELGNLTNLRE---------IYLANYNVFEGEIPVELSNLVNLVHMDLSSCGLD 244
Query: 1716 GPIPTTIGQLHQVSKLDLSGNSLSGEIPPDIGNCVHLTYLDLSQNNLSGSIPLEVSNIRI 1537
GPIP +G L + L L N LSG IP ++GN +L LDLS N L+G IP E N++
Sbjct: 245 GPIPNELGNLKLLHTLYLHINFLSGSIPKELGNLTNLVNLDLSYNALTGEIPFEFINLKQ 304
Query: 1536 LNYLNVSRNHLNDSIPKSIETMKSLTIADFSFNDLSGKLP 1417
LN LN+ N L+ SIP + + +L N+ +G++P
Sbjct: 305 LNLLNLFLNRLHGSIPDYVADLPNLETLQLWKNNFTGEIP 344
>ref|XP_003592924.1| Receptor-like protein kinase [Medicago truncatula]
gi|355481972|gb|AES63175.1| Receptor-like protein kinase
[Medicago truncatula]
Length = 1007
Score = 1092 bits (2824), Expect = 0.0
Identities = 541/750 (72%), Positives = 630/750 (84%)
Frame = -3
Query: 2514 GIPKEFGKLVNLVHMDLSSCGLDGPIPPELGNLHSVDTIFLHINLLSGPIPRQLGNLTSL 2335
GIP EFG+L LVHMD+SSC LDG IP ELGNL ++T++LHIN LSG IP+QLGNLT+L
Sbjct: 226 GIPMEFGRLTKLVHMDISSCDLDGSIPRELGNLKELNTLYLHINQLSGSIPKQLGNLTNL 285
Query: 2334 VYLDLSANALSGEIPXXXXXXXXXXXXXXXXNRLHGSIPDFVADYPDLQTLGLWSNNFTG 2155
+YLDLS+NAL+GEIP NRLHGSIPD++AD+PDL TLGLW NNFTG
Sbjct: 286 LYLDLSSNALTGEIPIEFINLNRLTLLNLFLNRLHGSIPDYIADFPDLDTLGLWMNNFTG 345
Query: 2154 VIPENLGRNQNLRELDLSTNKLTGTIPRDLCASNQLRVLILLKNFLFGSIPEDLGTCSSL 1975
IP LG N L+ LDLS+NKLTG IP LC+S+QL++LILL NFLFG IP+ LGTC SL
Sbjct: 346 EIPYKLGLNGKLQILDLSSNKLTGIIPPHLCSSSQLKILILLNNFLFGPIPQGLGTCYSL 405
Query: 1974 VRVRLGENYLNGSIPDGXXXXXXXXXXXLHNNYLSGNLSDNRDPSSRPVKXXXXXXXXXX 1795
RVRLGENYLNGSIP+G L NNYLSG LS+N + SS+PV
Sbjct: 406 TRVRLGENYLNGSIPNGFLYLPKLNLAELKNNYLSGTLSENGNSSSKPVSLEQLDLSNNA 465
Query: 1794 XSGYLPFSLSNFSSLQILLLAGNRFSGPIPTTIGQLHQVSKLDLSGNSLSGEIPPDIGNC 1615
SG LP+SLSNF+SLQILLL+GN+FSGPIP +IG L+QV KLDL+ NSLSG+IPP+IG C
Sbjct: 466 LSGPLPYSLSNFTSLQILLLSGNQFSGPIPPSIGGLNQVLKLDLTRNSLSGDIPPEIGYC 525
Query: 1614 VHLTYLDLSQNNLSGSIPLEVSNIRILNYLNVSRNHLNDSIPKSIETMKSLTIADFSFND 1435
VHLTYLD+SQNNLSGSIP +SNIRILNYLN+SRNHLN SIP+SI TMKSLT+ADFSFN+
Sbjct: 526 VHLTYLDMSQNNLSGSIPPLISNIRILNYLNLSRNHLNQSIPRSIGTMKSLTVADFSFNE 585
Query: 1434 LSGKLPESGQFTFFNASSFAGNPQLCGQLLNNPCNVSGISDSVNKKPGRFKLIFALGLLI 1255
SGKLPESGQF+FFNA+SFAGNP+LCG LLNNPC ++ + + K FKLIFALGLL+
Sbjct: 586 FSGKLPESGQFSFFNATSFAGNPKLCGSLLNNPCKLTRMKSTPGKNNSDFKLIFALGLLM 645
Query: 1254 CSLVFAAAAMVKAKSYRRSGSDVWKMTSFQKLEFTVADVLECIKDGNVIGRGGAGIVYHG 1075
CSLVFA AA++KAKS+++ G WKMT+F+KLEFTV+D+LEC+KDGNVIGRGGAGIVYHG
Sbjct: 646 CSLVFAVAAIIKAKSFKKKGPGSWKMTAFKKLEFTVSDILECVKDGNVIGRGGAGIVYHG 705
Query: 1074 KMPNGTEIAVKKLVGFGNTSHDHGFRAEIRTLGNIRHRNIVRLLAFCSNKDTNLLVYEYM 895
KMPNG EIAVKKL+GFG +HDHGFRAEI+TLGNIRHRNIVRLLAFCSNK+TNLLVYEYM
Sbjct: 706 KMPNGMEIAVKKLLGFGANNHDHGFRAEIQTLGNIRHRNIVRLLAFCSNKETNLLVYEYM 765
Query: 894 RNGSLGEALHAKIKGGILGWHLRYKISIEAARGLCYLHHDCSPLIVHRDVKSNNILLNSS 715
RNGSLGE LH K KG L W+ RYKISI++A+GLCYLHHDCSPLI+HRDVKSNNILL+S+
Sbjct: 766 RNGSLGETLHGK-KGAFLSWNFRYKISIDSAKGLCYLHHDCSPLILHRDVKSNNILLSSN 824
Query: 714 YEAHVADFGLAKYLVDGGASECMSAIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELI 535
+EAHVADFGLAK+LVDG A+ECMS+IAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLEL+
Sbjct: 825 FEAHVADFGLAKFLVDGAAAECMSSIAGSYGYIAPEYAYTLRVDEKSDVYSFGVVLLELL 884
Query: 534 TGKRPVGGFGDGVDIVQWARTSTRCRKEEVNCIIDSRLVNVPQEEAMHLFFIAMLCIQEN 355
TG++PVG FG+GVD+VQW + +T R+EEV IIDSRL+ VP+EEAMH+FFIAMLC++EN
Sbjct: 885 TGRKPVGDFGEGVDLVQWCKKATNGRREEVVNIIDSRLMVVPKEEAMHMFFIAMLCLEEN 944
Query: 354 SVERPTMREVVQMLSEFPRDSPENQTSSSS 265
SV+RPTMREVVQMLSEFPR S + +SSSS
Sbjct: 945 SVQRPTMREVVQMLSEFPRQSTSSSSSSSS 974
Score = 115 bits (289), Expect = 4e-23
Identities = 93/337 (27%), Positives = 144/337 (42%), Gaps = 1/337 (0%)
Frame = -3
Query: 2424 GNLHSVDTIFLHINLLSGPIPRQLGNLTSLVYLDLSANALSGEIPXXXXXXXXXXXXXXX 2245
G + S+D L+ L G + + +L L +L L+ N +G I
Sbjct: 67 GRVVSLDLTDLN---LFGSVSPSISSLDRLSHLSLAGNNFTGTI---------------- 107
Query: 2244 XNRLHGSIPDFVADYPDLQTLGLWSNNFTGVIPENLGRNQNLRELDLSTNKLTGTIPRDL 2065
+ + +LQ L + +N F+G + N +NL+ +D+ N T +P +
Sbjct: 108 ----------HITNLTNLQFLNISNNQFSGHMDWNYSTMENLQVVDVYNNNFTSLLPLGI 157
Query: 2064 CA-SNQLRVLILLKNFLFGSIPEDLGTCSSLVRVRLGENYLNGSIPDGXXXXXXXXXXXL 1888
+ N+L+ L L NF FG IP+ G SL + L N ++G IP
Sbjct: 158 LSLKNKLKHLDLGGNFFFGEIPKSYGKLVSLEYLSLAGNDISGKIPGEL----------- 206
Query: 1887 HNNYLSGNLSDNRDPSSRPVKXXXXXXXXXXXSGYLPFSLSNFSSLQILLLAGNRFSGPI 1708
GNLS+ R+ G +P + L + ++ G I
Sbjct: 207 ------GNLSNLRE---------IYLGYYNTYEGGIPMEFGRLTKLVHMDISSCDLDGSI 251
Query: 1707 PTTIGQLHQVSKLDLSGNSLSGEIPPDIGNCVHLTYLDLSQNNLSGSIPLEVSNIRILNY 1528
P +G L +++ L L N LSG IP +GN +L YLDLS N L+G IP+E N+ L
Sbjct: 252 PRELGNLKELNTLYLHINQLSGSIPKQLGNLTNLLYLDLSSNALTGEIPIEFINLNRLTL 311
Query: 1527 LNVSRNHLNDSIPKSIETMKSLTIADFSFNDLSGKLP 1417
LN+ N L+ SIP I L N+ +G++P
Sbjct: 312 LNLFLNRLHGSIPDYIADFPDLDTLGLWMNNFTGEIP 348
Score = 99.8 bits (247), Expect = 3e-18
Identities = 79/276 (28%), Positives = 121/276 (43%), Gaps = 2/276 (0%)
Frame = -3
Query: 2235 LHGSIPDFVADYPDLQTLGLWSNNFTGVIPENLGRNQNLRELDLSTNKLTGTIPRDLCAS 2056
L GS+ ++ L L L NNFTG I ++ NL+ L++S N+ +G + +
Sbjct: 79 LFGSVSPSISSLDRLSHLSLAGNNFTGTI--HITNLTNLQFLNISNNQFSGHMDWNYSTM 136
Query: 2055 NQLRVLILLKNFLFGSIPED-LGTCSSLVRVRLGENYLNGSIPDGXXXXXXXXXXXLHNN 1879
L+V+ + N +P L + L + LG N+ G IP L N
Sbjct: 137 ENLQVVDVYNNNFTSLLPLGILSLKNKLKHLDLGGNFFFGEIPKSYGKLVSLEYLSLAGN 196
Query: 1878 YLSGNLSDNRDPSSRPVKXXXXXXXXXXXSGYLPFSLSNFSSLQILLLAG-NRFSGPIPT 1702
+SG + P L N S+L+ + L N + G IP
Sbjct: 197 DISGKI---------------------------PGELGNLSNLREIYLGYYNTYEGGIPM 229
Query: 1701 TIGQLHQVSKLDLSGNSLSGEIPPDIGNCVHLTYLDLSQNNLSGSIPLEVSNIRILNYLN 1522
G+L ++ +D+S L G IP ++GN L L L N LSGSIP ++ N+ L YL+
Sbjct: 230 EFGRLTKLVHMDISSCDLDGSIPRELGNLKELNTLYLHINQLSGSIPKQLGNLTNLLYLD 289
Query: 1521 VSRNHLNDSIPKSIETMKSLTIADFSFNDLSGKLPE 1414
+S N L IP + LT+ + N L G +P+
Sbjct: 290 LSSNALTGEIPIEFINLNRLTLLNLFLNRLHGSIPD 325