BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00004754
         (1584 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAY22200.1| squalene monooxygenase [Datura inoxia]                 784   0.0  
gb|ADD17678.1| squalene epoxidase [Withania somnifera]                783   0.0  
ref|XP_002285493.1| PREDICTED: squalene monooxygenase isoform 1 ...   778   0.0  
gb|AGC94919.1| putative squaline epoxidase [Withania somnifera]       773   0.0  
ref|XP_002510043.1| Squalene monooxygenase, putative [Ricinus co...   769   0.0  

>gb|AAY22200.1| squalene monooxygenase [Datura inoxia]
          Length = 529

 Score =  784 bits (2024), Expect = 0.0
 Identities = 390/504 (77%), Positives = 435/504 (86%), Gaps = 3/504 (0%)
 Frame = +3

Query: 81   MVDQFVIGSAFASVVGFILIYAFFVVKNVRKALPADTGNNDGKSSSVTTKTICISGECRS 260
            MVD  + GS  A V+GF+ ++ FF+  N            D  +++ TT T    GE RS
Sbjct: 1    MVDLGLSGSVLAVVLGFVAVFWFFIQGNGG----GSKDKTDDPATTTTTTTTVYDGESRS 56

Query: 261  XXXXXXXXXXXXXXXXX---LAHTLGKDGRRVRVIERDLKEPDRIVGELLQPGGYLKLME 431
                                LAHTLGK+GRRV+VIERDL EPDRIVGELLQPGGYLKL E
Sbjct: 57   KDGNDDVDIIIVGAGVAGAALAHTLGKEGRRVKVIERDLTEPDRIVGELLQPGGYLKLQE 116

Query: 432  LGLEDCVEDIDAQRVFGYALFKDGKNTRLSYPLERFHSDVAGRSFHNGRFIQKMREKASL 611
            LGLEDCVE IDAQRVFGYALFKDGK+TRLSYPLE+FHSDVAGRSFHNGRFIQ+MREKA+ 
Sbjct: 117  LGLEDCVEKIDAQRVFGYALFKDGKSTRLSYPLEKFHSDVAGRSFHNGRFIQRMREKAAS 176

Query: 612  LPNVQLEQGTVSSLLEDNGTITGVMYKIKTGEVMKAYAPLTIVCDGCFSNLRGSLCNPQV 791
            LPNV+LEQGTV+SLLE+NGTI GV YK K+GE +KAYAPLT+VCDGCFSNLR +LC+P+V
Sbjct: 177  LPNVKLEQGTVTSLLEENGTIKGVQYKTKSGEELKAYAPLTVVCDGCFSNLRRTLCDPKV 236

Query: 792  DVPSCFVGLILENCRLPYENHGHVILADPSPILFYRISSTEIRCLVDVPGQKVPSISNGD 971
            +VPSCFVGL+LENC+LP+ENHGHVILADPSPILFY ISSTE+RCLVDVPGQKVPSISNG+
Sbjct: 237  EVPSCFVGLVLENCQLPHENHGHVILADPSPILFYPISSTEVRCLVDVPGQKVPSISNGE 296

Query: 972  LSKYLKTVVAPQIPPEMQDAFVATIDKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHP 1151
            ++KYLK VVAPQ+P E++DAF+A I+KGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHP
Sbjct: 297  MAKYLKNVVAPQVPAEIKDAFIAAINKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHP 356

Query: 1152 LTGGGMTVALSDIVVLRNLLMPLHDLHDSYTLCKYLESFYTLRKPVASTINTLAGALYKV 1331
            LTGGGMTVALSDIVVLR+LL PLHDL+D+ TLC+YLESFYTLRKPVASTINTLAGALYKV
Sbjct: 357  LTGGGMTVALSDIVVLRDLLKPLHDLNDAPTLCRYLESFYTLRKPVASTINTLAGALYKV 416

Query: 1332 FCASPDEARQEMREACFDYLSLGGVFSNGPVSLLSGLNPRPLSLVAHFFAVAIYGVGRLL 1511
            FCASPD+AR+EMREACFDYLSLGGVFS GPVSLLSGLNPRPLSLV HFFAVAIYGVGRLL
Sbjct: 417  FCASPDQARKEMREACFDYLSLGGVFSEGPVSLLSGLNPRPLSLVCHFFAVAIYGVGRLL 476

Query: 1512 LPFPSPKRMWMGARLISGASGIIF 1583
            LPFPSPKR+W+GARLISGAS IIF
Sbjct: 477  LPFPSPKRLWIGARLISGASAIIF 500


>gb|ADD17678.1| squalene epoxidase [Withania somnifera]
          Length = 531

 Score =  783 bits (2023), Expect = 0.0
 Identities = 390/504 (77%), Positives = 438/504 (86%), Gaps = 3/504 (0%)
 Frame = +3

Query: 81   MVDQFVIGSAFASVVGFILIYAFFVVKNVRKALPADTGNNDGKSSSVTTKTICISGECR- 257
            MV   + GS  A VVGFI ++ FF+ K+  ++   D  +    +++ TT T    GE R 
Sbjct: 1    MVVLSLSGSVLALVVGFIAVFCFFIQKSSGQS--NDKIDESATTTTTTTTTTVYDGESRP 58

Query: 258  --SXXXXXXXXXXXXXXXXXLAHTLGKDGRRVRVIERDLKEPDRIVGELLQPGGYLKLME 431
                                LAHTLGK+GRRV+VIERDL EPDRIVGELLQPGGYLKL E
Sbjct: 59   KDGNDDVDIIVVGAGVAGAALAHTLGKEGRRVKVIERDLTEPDRIVGELLQPGGYLKLQE 118

Query: 432  LGLEDCVEDIDAQRVFGYALFKDGKNTRLSYPLERFHSDVAGRSFHNGRFIQKMREKASL 611
            LGLEDCVE IDAQRVFGYALFKDGK+TRLSYPLE+FHSDV+GRSFHNGRFIQ+MREKA+ 
Sbjct: 119  LGLEDCVEKIDAQRVFGYALFKDGKSTRLSYPLEKFHSDVSGRSFHNGRFIQRMREKAAS 178

Query: 612  LPNVQLEQGTVSSLLEDNGTITGVMYKIKTGEVMKAYAPLTIVCDGCFSNLRGSLCNPQV 791
            LPNV+LEQGTV+SLLE+NGTI GV YK K+GE +KAYAPLTIVCDGCFSNLR +LC+P+V
Sbjct: 179  LPNVKLEQGTVTSLLEENGTIRGVQYKTKSGEELKAYAPLTIVCDGCFSNLRRTLCDPKV 238

Query: 792  DVPSCFVGLILENCRLPYENHGHVILADPSPILFYRISSTEIRCLVDVPGQKVPSISNGD 971
            +VPSCFVGL+LENC+LP+ENHGHVILADPSPILFY ISSTE+RCLVDVPGQKVPSISNG+
Sbjct: 239  EVPSCFVGLVLENCQLPHENHGHVILADPSPILFYPISSTEVRCLVDVPGQKVPSISNGE 298

Query: 972  LSKYLKTVVAPQIPPEMQDAFVATIDKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHP 1151
            ++KYLKTVVAPQ+P E++DAF+A +DKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHP
Sbjct: 299  MAKYLKTVVAPQVPSEIKDAFIAAVDKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHP 358

Query: 1152 LTGGGMTVALSDIVVLRNLLMPLHDLHDSYTLCKYLESFYTLRKPVASTINTLAGALYKV 1331
            LTGGGMTVALSDIVVLR+LL PL DL+D+ TLC+YLESFYTLRKPVASTINTLAGALYKV
Sbjct: 359  LTGGGMTVALSDIVVLRDLLKPLRDLNDAPTLCRYLESFYTLRKPVASTINTLAGALYKV 418

Query: 1332 FCASPDEARQEMREACFDYLSLGGVFSNGPVSLLSGLNPRPLSLVAHFFAVAIYGVGRLL 1511
            FCASPD+AR+EMREACFDYLSLGGVFS+GPVSLLSGLNPRPLSLV HFFAVAIYGVGRLL
Sbjct: 419  FCASPDQARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLVCHFFAVAIYGVGRLL 478

Query: 1512 LPFPSPKRMWMGARLISGASGIIF 1583
            LPFPSPKR+W+GARLISGAS IIF
Sbjct: 479  LPFPSPKRLWIGARLISGASAIIF 502


>ref|XP_002285493.1| PREDICTED: squalene monooxygenase isoform 1 [Vitis vinifera]
            gi|302142152|emb|CBI19355.3| unnamed protein product
            [Vitis vinifera]
          Length = 523

 Score =  778 bits (2008), Expect = 0.0
 Identities = 390/508 (76%), Positives = 438/508 (86%), Gaps = 7/508 (1%)
 Frame = +3

Query: 81   MVDQFVIGSAFASVVGFILIYAFFVVKNVRKALPADTGNNDGKSSSVTTKTICI----SG 248
            MVDQ ++G  FAS++GF+ ++   ++KN +K             +SV  +  C+    +G
Sbjct: 1    MVDQGILGWIFASLLGFV-VFRSVLLKNKKKG-----------GASVEIRDECVKSTANG 48

Query: 249  ECRSXXXXXXXXXXXXXXXXX---LAHTLGKDGRRVRVIERDLKEPDRIVGELLQPGGYL 419
            ECRS                    LA+TLGKDGRRVRVIERDL EPDRIVGELLQPGGYL
Sbjct: 49   ECRSKTACEEADVIIVGAGVAGAALANTLGKDGRRVRVIERDLTEPDRIVGELLQPGGYL 108

Query: 420  KLMELGLEDCVEDIDAQRVFGYALFKDGKNTRLSYPLERFHSDVAGRSFHNGRFIQKMRE 599
            KL+ELGL+DCVE+IDAQRVFGYALFKDGKNT+LSYPLE+F SDVAGRSFHNGRFIQ+MRE
Sbjct: 109  KLIELGLQDCVEEIDAQRVFGYALFKDGKNTKLSYPLEKFDSDVAGRSFHNGRFIQRMRE 168

Query: 600  KASLLPNVQLEQGTVSSLLEDNGTITGVMYKIKTGEVMKAYAPLTIVCDGCFSNLRGSLC 779
            +A+ LPNVQLEQGTV+SLLE+ GTI GV YK K GE M AYAPLTIVCDGCFSNLR SLC
Sbjct: 169  RAATLPNVQLEQGTVTSLLEEKGTIRGVNYKTKDGETMTAYAPLTIVCDGCFSNLRRSLC 228

Query: 780  NPQVDVPSCFVGLILENCRLPYENHGHVILADPSPILFYRISSTEIRCLVDVPGQKVPSI 959
             P+VDVPSCFVGL+LE+C LP+ NHGHV+LADPSPILFYRISSTEIRCLVDVPGQKVPSI
Sbjct: 229  TPKVDVPSCFVGLLLEDCELPFANHGHVVLADPSPILFYRISSTEIRCLVDVPGQKVPSI 288

Query: 960  SNGDLSKYLKTVVAPQIPPEMQDAFVATIDKGNIRTMPNRSMPAAPHPTPGALLMGDAFN 1139
            SNG+++KYLKTVVAPQIPPE+ D F+A I+KGNIRTMPNRSMPAAPHPTPGALLMGDAFN
Sbjct: 289  SNGEMAKYLKTVVAPQIPPELYDGFIAAINKGNIRTMPNRSMPAAPHPTPGALLMGDAFN 348

Query: 1140 MRHPLTGGGMTVALSDIVVLRNLLMPLHDLHDSYTLCKYLESFYTLRKPVASTINTLAGA 1319
            MRHPLTGGGMTVALSDIVVLR+LL PLHDL+D+ TLCKYLESFYTLRKPVASTINTLAGA
Sbjct: 349  MRHPLTGGGMTVALSDIVVLRDLLRPLHDLNDAATLCKYLESFYTLRKPVASTINTLAGA 408

Query: 1320 LYKVFCASPDEARQEMREACFDYLSLGGVFSNGPVSLLSGLNPRPLSLVAHFFAVAIYGV 1499
            LY+VFCASPD+AR+EMR+ACFDYLSLGGV S+GPVSLLSGLNPRPLSLV HFFAVAI+GV
Sbjct: 409  LYRVFCASPDQARKEMRDACFDYLSLGGVCSSGPVSLLSGLNPRPLSLVCHFFAVAIFGV 468

Query: 1500 GRLLLPFPSPKRMWMGARLISGASGIIF 1583
            GRLLLPFPSPKR+W+GAR+ISGASGIIF
Sbjct: 469  GRLLLPFPSPKRVWIGARIISGASGIIF 496


>gb|AGC94919.1| putative squaline epoxidase [Withania somnifera]
          Length = 524

 Score =  773 bits (1996), Expect = 0.0
 Identities = 385/504 (76%), Positives = 431/504 (85%), Gaps = 3/504 (0%)
 Frame = +3

Query: 81   MVDQFVIGSAFASVVGFILIYAFFVVKNVRKALPADTGNNDGKSSSVTTKTICISGECRS 260
            MVD  + GSA A V+GFI ++ FF+ +N         G+    S++ TT T    G+ RS
Sbjct: 1    MVDLGLNGSALAVVLGFIAVFWFFIQRN---------GDKIDDSTTTTTTTTLYDGQSRS 51

Query: 261  XXXXXXXXXXXXXXXXX---LAHTLGKDGRRVRVIERDLKEPDRIVGELLQPGGYLKLME 431
                                LAHTLGK+GRRV+VIERDL EPDRIVGELLQPGGYLKL E
Sbjct: 52   KNGNDDVDIIVVGAGVAGAALAHTLGKEGRRVKVIERDLTEPDRIVGELLQPGGYLKLQE 111

Query: 432  LGLEDCVEDIDAQRVFGYALFKDGKNTRLSYPLERFHSDVAGRSFHNGRFIQKMREKASL 611
            LGLEDCVE IDAQRVFGYALFKDGK+TRLSYPLE+FHSDV+GRSFHNGRFIQ+MREKA+ 
Sbjct: 112  LGLEDCVEKIDAQRVFGYALFKDGKSTRLSYPLEKFHSDVSGRSFHNGRFIQRMREKAAS 171

Query: 612  LPNVQLEQGTVSSLLEDNGTITGVMYKIKTGEVMKAYAPLTIVCDGCFSNLRGSLCNPQV 791
            LPNV+LEQGTV+SLLE+NGTI GV YK K+GE +K YAPLTIVCDGCFSNLR +LC+P+V
Sbjct: 172  LPNVKLEQGTVTSLLEENGTIRGVQYKTKSGEELKGYAPLTIVCDGCFSNLRRTLCDPKV 231

Query: 792  DVPSCFVGLILENCRLPYENHGHVILADPSPILFYRISSTEIRCLVDVPGQKVPSISNGD 971
            +VPSCFVGL+LENC+LP+ENHGHVILADPSPILFY ISSTE+RCLVDVPGQKVPSI+NG+
Sbjct: 232  EVPSCFVGLVLENCQLPHENHGHVILADPSPILFYPISSTEVRCLVDVPGQKVPSIANGE 291

Query: 972  LSKYLKTVVAPQIPPEMQDAFVATIDKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHP 1151
            ++KYLK VVAPQ+PPE++DAF+A + KGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHP
Sbjct: 292  MAKYLKMVVAPQVPPEIKDAFIAAVGKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHP 351

Query: 1152 LTGGGMTVALSDIVVLRNLLMPLHDLHDSYTLCKYLESFYTLRKPVASTINTLAGALYKV 1331
            LTGGGMTVALSDIVVLR+LL PLHDL+D+ TLC+YLESFYTLRKPVA TINTLAGALYKV
Sbjct: 352  LTGGGMTVALSDIVVLRDLLKPLHDLNDAPTLCRYLESFYTLRKPVAPTINTLAGALYKV 411

Query: 1332 FCASPDEARQEMREACFDYLSLGGVFSNGPVSLLSGLNPRPLSLVAHFFAVAIYGVGRLL 1511
            FCASPD+AR+EMREACFDYLSLGGVFS GPVSLLSGLNP PLSLV HFFA AIYGVGRLL
Sbjct: 412  FCASPDQARKEMREACFDYLSLGGVFSEGPVSLLSGLNPHPLSLVCHFFAEAIYGVGRLL 471

Query: 1512 LPFPSPKRMWMGARLISGASGIIF 1583
            L FPSPKR+WMG RLIS AS IIF
Sbjct: 472  LTFPSPKRIWMGVRLISSASAIIF 495


>ref|XP_002510043.1| Squalene monooxygenase, putative [Ricinus communis]
            gi|223550744|gb|EEF52230.1| Squalene monooxygenase,
            putative [Ricinus communis]
          Length = 534

 Score =  770 bits (1987), Expect = 0.0
 Identities = 385/510 (75%), Positives = 433/510 (84%), Gaps = 9/510 (1%)
 Frame = +3

Query: 81   MVDQFVIGSAFASVVGFILIY--AFFVVK-----NVRKALPADTGNNDGKSSSVTTKTIC 239
            M D +++G    S++G   +Y   + VVK     N RKAL     +     S+ T   + 
Sbjct: 1    MADNYLLGWILCSIIGLFGLYYMVYLVVKREEEDNNRKALLQARSD-----SAKTMSAVS 55

Query: 240  ISGECRSXXXXXXXXXXXXXXXXX--LAHTLGKDGRRVRVIERDLKEPDRIVGELLQPGG 413
             +GECRS                   LAHTLGKDGRRV VIERDL EPDRIVGELLQPGG
Sbjct: 56   QNGECRSDNPADADIIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGG 115

Query: 414  YLKLMELGLEDCVEDIDAQRVFGYALFKDGKNTRLSYPLERFHSDVAGRSFHNGRFIQKM 593
            YLKL+ELGLEDCVE+IDAQRVFGYALF DGK+T+LSYPLE+FHSDVAGRSFHNGRFIQ+M
Sbjct: 116  YLKLIELGLEDCVEEIDAQRVFGYALFMDGKHTQLSYPLEKFHSDVAGRSFHNGRFIQRM 175

Query: 594  REKASLLPNVQLEQGTVSSLLEDNGTITGVMYKIKTGEVMKAYAPLTIVCDGCFSNLRGS 773
            REKAS +PNV+LEQGTV+SL+E+ G I GV+YK KTGE + A+APLTIVCDGCFSNLR S
Sbjct: 176  REKASSIPNVRLEQGTVTSLIEEKGIIRGVVYKTKTGEELTAFAPLTIVCDGCFSNLRRS 235

Query: 774  LCNPQVDVPSCFVGLILENCRLPYENHGHVILADPSPILFYRISSTEIRCLVDVPGQKVP 953
            LCNP+VDVPSCFVGL+LE+C+LPY+ HGHV+LADPSPILFY+ISSTE+RCLVDVPGQKVP
Sbjct: 236  LCNPKVDVPSCFVGLVLEDCKLPYQYHGHVVLADPSPILFYQISSTEVRCLVDVPGQKVP 295

Query: 954  SISNGDLSKYLKTVVAPQIPPEMQDAFVATIDKGNIRTMPNRSMPAAPHPTPGALLMGDA 1133
            SISNG+++KYLK VVAPQ+PPE+ D+FVA +DKGNIRTMPNRSMPA+P+PTPGALLMGDA
Sbjct: 296  SISNGEMAKYLKNVVAPQVPPEIYDSFVAAVDKGNIRTMPNRSMPASPYPTPGALLMGDA 355

Query: 1134 FNMRHPLTGGGMTVALSDIVVLRNLLMPLHDLHDSYTLCKYLESFYTLRKPVASTINTLA 1313
            FNMRHPLTGGGMTVALSDIVVLR LL PL DLHD+ TLC+YLESFYTLRKPVASTINTLA
Sbjct: 356  FNMRHPLTGGGMTVALSDIVVLRELLKPLRDLHDAPTLCRYLESFYTLRKPVASTINTLA 415

Query: 1314 GALYKVFCASPDEARQEMREACFDYLSLGGVFSNGPVSLLSGLNPRPLSLVAHFFAVAIY 1493
            GALYKVFCAS DEAR EMR+ACFDYLSLGGVFS GP+SLLSGLNPRPLSLV HFFAVAIY
Sbjct: 416  GALYKVFCASSDEARNEMRQACFDYLSLGGVFSTGPISLLSGLNPRPLSLVVHFFAVAIY 475

Query: 1494 GVGRLLLPFPSPKRMWMGARLISGASGIIF 1583
            GVGRLLLPFPSPKR+W+GARLISGASGIIF
Sbjct: 476  GVGRLLLPFPSPKRVWVGARLISGASGIIF 505