BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00004732
         (1095 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADG02944.1| putative C-8,7 sterol isomerase [Gossypium hirsutum]   337   4e-90
ref|XP_002285453.1| PREDICTED: probable 3-beta-hydroxysteroid-De...   336   5e-90
gb|ABU95101.1| putative C-8,7 sterol isomerase [Solanum tuberosum]    336   7e-90
gb|AFK46718.1| unknown [Lotus japonicus]                              315   1e-83
ref|XP_002512695.1| sterol isomerase, putative [Ricinus communis...   315   1e-83

>gb|ADG02944.1| putative C-8,7 sterol isomerase [Gossypium hirsutum]
          Length = 217

 Score =  337 bits (863), Expect = 4e-90
 Identities = 158/215 (73%), Positives = 181/215 (84%)
 Frame = +2

Query: 83  HPFIPRDLVLQDYVPITLSQSTILTVYGCASFLVVSFVWIIAGRFPKISKLDKVLMCWWV 262
           HP++PRDL L  YVP++LSQSTILTVYG +S LVVS VW ++GR   ISKLD++LMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 263 FTGLTHMILEGYFVFTPEFFKQKTPTYLAEVWKEYSKGDSRYVGRDSAIVAVEGITAVLE 442
           FTGLTH+ILEGYF F+PEF+K KT  YLAEVWKEYSKGDSRY GRDS IVAVEGIT+VLE
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSTIVAVEGITSVLE 122

Query: 443 GPACLLAAYAIATRKSYSHLLQIAISLGQLYGTAVYFITSYLQGDNFSVSPFYYYAYYII 622
           GP CLLA YAIA  K YS++LQ AISLGQLYGT VYFIT+YL+GDNFS SPFYYYAYY++
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 623 ANSFWIWIPSLVMIRSWKTICAAVHIQEQKKSKTR 727
           ANSFW+ IPSL+ IR WK I +AV  Q QKK+K R
Sbjct: 183 ANSFWLLIPSLIAIRCWKKISSAVQSQSQKKNKIR 217


>ref|XP_002285453.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           isoform 2 [Vitis vinifera]
           gi|225435456|ref|XP_002285450.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           isoform 1 [Vitis vinifera] gi|297746323|emb|CBI16379.3|
           unnamed protein product [Vitis vinifera]
          Length = 222

 Score =  336 bits (862), Expect = 5e-90
 Identities = 152/222 (68%), Positives = 186/222 (83%)
 Frame = +2

Query: 62  VQASESIHPFIPRDLVLQDYVPITLSQSTILTVYGCASFLVVSFVWIIAGRFPKISKLDK 241
           ++ +   HP++P+DL L  YVP+ LSQSTI+ VYG +S LVVS +WII+GRFPK SK+D+
Sbjct: 1   MEGAPQSHPYVPKDLKLPGYVPVVLSQSTIVGVYGLSSLLVVSVIWIISGRFPKTSKIDR 60

Query: 242 VLMCWWVFTGLTHMILEGYFVFTPEFFKQKTPTYLAEVWKEYSKGDSRYVGRDSAIVAVE 421
           +LMCWW FTGLTH ILEGYFVF+PEF+K KT  YLAEVWKEYSKGDSRY  RD+ ++AVE
Sbjct: 61  LLMCWWAFTGLTHAILEGYFVFSPEFYKDKTACYLAEVWKEYSKGDSRYAARDAGVIAVE 120

Query: 422 GITAVLEGPACLLAAYAIATRKSYSHLLQIAISLGQLYGTAVYFITSYLQGDNFSVSPFY 601
           GITAVLEGPA LLA Y IAT++SYS++LQ+ ISLGQLYGTAVYFITSYL GDNF+ S +Y
Sbjct: 121 GITAVLEGPASLLALYTIATKRSYSYILQLTISLGQLYGTAVYFITSYLDGDNFAASAYY 180

Query: 602 YYAYYIIANSFWIWIPSLVMIRSWKTICAAVHIQEQKKSKTR 727
           YYAYY+ AN+FW+ IPSL++IR WK ICAA  +Q Q+K+K R
Sbjct: 181 YYAYYVFANAFWVVIPSLIVIRCWKKICAAFQVQGQQKTKVR 222


>gb|ABU95101.1| putative C-8,7 sterol isomerase [Solanum tuberosum]
          Length = 221

 Score =  336 bits (861), Expect = 7e-90
 Identities = 148/215 (68%), Positives = 187/215 (86%)
 Frame = +2

Query: 83  HPFIPRDLVLQDYVPITLSQSTILTVYGCASFLVVSFVWIIAGRFPKISKLDKVLMCWWV 262
           HP++P DL L  +VP+ LSQS I+ VYG +S LVV F+W+ +G  PK+SK D+VLMCWW+
Sbjct: 8   HPYVPHDLKLPGFVPLFLSQSEIVGVYGISSLLVVVFMWVFSGFVPKLSKTDRVLMCWWI 67

Query: 263 FTGLTHMILEGYFVFTPEFFKQKTPTYLAEVWKEYSKGDSRYVGRDSAIVAVEGITAVLE 442
           FTGLTHM+LEGYFVFTP+F+++ +P YLAEVWKEYSKGDSRYVGRD+A+++VEG+TAV+E
Sbjct: 68  FTGLTHMVLEGYFVFTPDFYQKTSPVYLAEVWKEYSKGDSRYVGRDAAVLSVEGVTAVIE 127

Query: 443 GPACLLAAYAIATRKSYSHLLQIAISLGQLYGTAVYFITSYLQGDNFSVSPFYYYAYYII 622
           GPACLLA YAIAT+K+Y H+LQ++I LGQLYGTA+YFIT+ L+GDNFS SPFYYYAYY+ 
Sbjct: 128 GPACLLAVYAIATKKAYRHVLQLSICLGQLYGTAIYFITAVLEGDNFSTSPFYYYAYYVF 187

Query: 623 ANSFWIWIPSLVMIRSWKTICAAVHIQEQKKSKTR 727
           AN FW+WIPSL++I  WK ICAAV++ EQ K+KTR
Sbjct: 188 ANHFWVWIPSLIVIHCWKKICAAVNVHEQ-KTKTR 221


>gb|AFK46718.1| unknown [Lotus japonicus]
          Length = 225

 Score =  315 bits (808), Expect = 1e-83
 Identities = 154/226 (68%), Positives = 181/226 (80%), Gaps = 1/226 (0%)
 Frame = +2

Query: 53  MAGVQASESIHPFIPRDLVLQDYVPITLSQSTILTVYGCASFLVVSFVWIIAGRFPKISK 232
           M G Q +   HP++PRDL L DYVP  LSQS+IL+V+  ++ LVVSF+WI +GR PK SK
Sbjct: 1   MEGSQVAHR-HPYVPRDLHLPDYVPCFLSQSSILSVFAISTLLVVSFIWIFSGRLPKKSK 59

Query: 233 LDKVLMCWWVFTGLTHMILEGYFVFTPEFFKQKTPTYLAEVWKEYSKGDSRYVGRDSAIV 412
           +D+VLMCWW FTGLTH+ILEGYFVF PEFFK KT  YLAEVWKEYSKGDSRYVGRD+ +V
Sbjct: 60  VDRVLMCWWAFTGLTHIILEGYFVFAPEFFKDKTGFYLAEVWKEYSKGDSRYVGRDAGVV 119

Query: 413 AVEGITAVLEGPACLLAAYAIATRKSYSHLLQIAISLGQLYGTAVYFITSYLQGDNFSVS 592
            VEG+TAV+EGPA LLA YAIAT KSYS++LQ AISLGQLYGTAVY+IT+ L+GDNFS +
Sbjct: 120 TVEGLTAVIEGPASLLAVYAIATGKSYSYMLQFAISLGQLYGTAVYYITAILEGDNFSTN 179

Query: 593 PFYYYAYYIIANSFWIWIPSLVMIRSWKTICAAVHIQEQ-KKSKTR 727
            FYYYAYYI AN  WI IPS++ IR W+   AA  +Q Q KK K R
Sbjct: 180 SFYYYAYYIGANFSWIVIPSIIAIRCWRKTSAAFRVQGQTKKPKVR 225


>ref|XP_002512695.1| sterol isomerase, putative [Ricinus communis]
           gi|223548656|gb|EEF50147.1| sterol isomerase, putative
           [Ricinus communis]
          Length = 220

 Score =  315 bits (808), Expect = 1e-83
 Identities = 147/214 (68%), Positives = 178/214 (83%)
 Frame = +2

Query: 83  HPFIPRDLVLQDYVPITLSQSTILTVYGCASFLVVSFVWIIAGRFPKISKLDKVLMCWWV 262
           HP++P DL L+DYVP+ LSQSTIL V+G +S ++VS VW I+G   K +K+D+ LMCWW 
Sbjct: 6   HPYVPTDLKLEDYVPVFLSQSTILAVFGTSSLVLVSLVWFISGSSRKAAKIDRWLMCWWA 65

Query: 263 FTGLTHMILEGYFVFTPEFFKQKTPTYLAEVWKEYSKGDSRYVGRDSAIVAVEGITAVLE 442
           FTGLTH+ILEGYFVF+PEF+K KT  YLAEVWKEYSKGDSRY  RDSA+VAVEG+TAVLE
Sbjct: 66  FTGLTHIILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARDSAVVAVEGLTAVLE 125

Query: 443 GPACLLAAYAIATRKSYSHLLQIAISLGQLYGTAVYFITSYLQGDNFSVSPFYYYAYYII 622
           GPA LLA YAIA  KSYS++LQ AISLGQLYGTAVYF+T YL G N++ SP+Y+YAYYI 
Sbjct: 126 GPASLLAVYAIAAGKSYSYILQFAISLGQLYGTAVYFLTPYLDGYNYASSPYYHYAYYIG 185

Query: 623 ANSFWIWIPSLVMIRSWKTICAAVHIQEQKKSKT 724
           AN+ W+ IPSL+ IRSWK ICAA  +Q Q+K+K+
Sbjct: 186 ANASWVVIPSLICIRSWKKICAAFQVQGQRKTKS 219