BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00003224
         (1654 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABC94481.1| putative steroid 23-alpha-hydroxylase cytochrome ...   795   0.0  
dbj|BAE16977.1| steroid 23-alpha-hydroxylase [Zinnia elegans]         756   0.0  
dbj|BAE16978.1| steroid 23-alpha-hydroxylase [Zinnia elegans]         734   0.0  
gb|AAZ39038.1| cytochrome P450 90A2 [Camellia japonica]               699   0.0  
emb|CAD27417.1| cytochrome P450 [Nicotiana tabacum]                   679   0.0  

>gb|ABC94481.1| putative steroid 23-alpha-hydroxylase cytochrome P450 [Artemisia
            annua]
          Length = 473

 Score =  795 bits (2053), Expect = 0.0
 Identities = 395/457 (86%), Positives = 422/457 (92%)
 Frame = -2

Query: 1464 IIFLVFRITTASRRKISMPPGSIGLPLIGETFQLISAYKTENPEPFIDTRVSKYGTVFTT 1285
            IIFL+FR  +A  R I+ PPGS GLPLIGETFQ+ISAYKTENPEPFID RV KYG+VFTT
Sbjct: 17   IIFLLFRRNSAFWRSINTPPGSTGLPLIGETFQIISAYKTENPEPFIDARVXKYGSVFTT 76

Query: 1284 HVFGERTVFSADAETNRFILQNEGRLFESSYPGSISNLVGKHSLLLMRGSLHRRMHSLTM 1105
            HVFGERTVFSAD E N+FILQNEGRLFESSYPGSI+NLVGKHSLLLMRG LHRRMHSLTM
Sbjct: 77   HVFGERTVFSADVEINKFILQNEGRLFESSYPGSIANLVGKHSLLLMRGGLHRRMHSLTM 136

Query: 1104 SFANSTIIKDHLLVDIDRLVRLNLDSWTGRILLMDEAKKITFELTLKQLLSIDPCEWTEN 925
            SFANSTIIKDHLL DIDRLVRLNLDSWTGRILLMDEAKKITFELTLKQLLSI+PCEW+EN
Sbjct: 137  SFANSTIIKDHLLADIDRLVRLNLDSWTGRILLMDEAKKITFELTLKQLLSIEPCEWSEN 196

Query: 924  LRKEYMLVIEGFFCIPLPFFSLTYLRAIQARKRVTEALNLVVXXXXXXXXRGVKKNDMLA 745
            LRKEYMLVIEGFFCIPLPFFSLTY RAIQARKRVTEAL+LVV        +GVKKNDMLA
Sbjct: 197  LRKEYMLVIEGFFCIPLPFFSLTYRRAIQARKRVTEALHLVVRERRIEREKGVKKNDMLA 256

Query: 744  ALFDSDGEGVIFRDDEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPLALAELKEEHDEI 565
            ALFDSDG+   F DDEIVDFLVSLLVAGYDTTSTTMTLAVK+LTDTP ALA+LKEEHD I
Sbjct: 257  ALFDSDGDEENFYDDEIVDFLVSLLVAGYDTTSTTMTLAVKYLTDTPSALAQLKEEHDAI 316

Query: 564  RARKGTSVALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVQIKGYTIPKGSKVF 385
            RA+KG SVALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRA+TDV+IK YTIPKG+KVF
Sbjct: 317  RAKKGASVALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAITDVKIKDYTIPKGAKVF 376

Query: 384  TSLRAVHLGQDNFEDARIFDPWRWQKTSDPANFMPFGGGPRRCPGYELARVALSVFLHHL 205
            TSLRAVHL QDNFEDAR+F+PWRWQKTSD A+FMPFGGGPRRCPG+ELARVALSVFLHHL
Sbjct: 377  TSLRAVHLDQDNFEDARVFNPWRWQKTSDSASFMPFGGGPRRCPGHELARVALSVFLHHL 436

Query: 204  VTRFCWKPAEEDKLVFFPTTRTKKRYPIIVQHRNMVG 94
            +T F WKPAEEDK+VFFPTTRT+KRYPIIV+ RN+VG
Sbjct: 437  ITHFSWKPAEEDKVVFFPTTRTQKRYPIIVEQRNVVG 473


>dbj|BAE16977.1| steroid 23-alpha-hydroxylase [Zinnia elegans]
          Length = 471

 Score =  756 bits (1952), Expect = 0.0
 Identities = 380/455 (83%), Positives = 407/455 (89%)
 Frame = -2

Query: 1461 IFLVFRITTASRRKISMPPGSIGLPLIGETFQLISAYKTENPEPFIDTRVSKYGTVFTTH 1282
            I ++   TT+SRR    PPG+ G+P IGET QLISAYKTENPEPFIDTRV KYG VFTTH
Sbjct: 21   ILIILHTTTSSRRNHHSPPGATGIPFIGETLQLISAYKTENPEPFIDTRVEKYGVVFTTH 80

Query: 1281 VFGERTVFSADAETNRFILQNEGRLFESSYPGSISNLVGKHSLLLMRGSLHRRMHSLTMS 1102
            VFGERTVFSAD ETNRFIL+NEGRLFESSYPGSI+NLVGK+SLLLMRG LHRRMHSLTMS
Sbjct: 81   VFGERTVFSADGETNRFILKNEGRLFESSYPGSIANLVGKNSLLLMRGGLHRRMHSLTMS 140

Query: 1101 FANSTIIKDHLLVDIDRLVRLNLDSWTGRILLMDEAKKITFELTLKQLLSIDPCEWTENL 922
            FANSTIIKDHLLVDIDRLVRLNLD+WTGRILLMDEAKKITFELTLKQLLSI+P EW+ENL
Sbjct: 141  FANSTIIKDHLLVDIDRLVRLNLDAWTGRILLMDEAKKITFELTLKQLLSIEPFEWSENL 200

Query: 921  RKEYMLVIEGFFCIPLPFFSLTYLRAIQARKRVTEALNLVVXXXXXXXXRGVKKNDMLAA 742
            RKEYMLVIEGFFCIPLPFF LTY RAIQARKRVTEAL+LVV        RGVKKNDMLAA
Sbjct: 201  RKEYMLVIEGFFCIPLPFFPLTYRRAIQARKRVTEALHLVVRERRVERERGVKKNDMLAA 260

Query: 741  LFDSDGEGVIFRDDEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPLALAELKEEHDEIR 562
            LFD+D +     DDEIVD LVSLLVAGYDTT TTMTLAVK+LTDTP ALA+LKEEH++I 
Sbjct: 261  LFDNDCD-----DDEIVDLLVSLLVAGYDTTPTTMTLAVKYLTDTPSALAQLKEEHEQIT 315

Query: 561  ARKGTSVALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVQIKGYTIPKGSKVFT 382
             +KG  VALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDV+IK +TIPKGSKVF 
Sbjct: 316  RKKGALVALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVKIKDFTIPKGSKVFA 375

Query: 381  SLRAVHLGQDNFEDARIFDPWRWQKTSDPANFMPFGGGPRRCPGYELARVALSVFLHHLV 202
            SLRAVHL  +NFEDAR+F PWRWQK SDPANFMPFGGGPRRCPG+ELARVALSVFLHHL+
Sbjct: 376  SLRAVHLDLENFEDARVFYPWRWQKISDPANFMPFGGGPRRCPGHELARVALSVFLHHLI 435

Query: 201  TRFCWKPAEEDKLVFFPTTRTKKRYPIIVQHRNMV 97
            TRF W PAEEDKLVFFPTTRT+KRYPIIVQ RN+V
Sbjct: 436  TRFSWIPAEEDKLVFFPTTRTQKRYPIIVQKRNVV 470


>dbj|BAE16978.1| steroid 23-alpha-hydroxylase [Zinnia elegans]
          Length = 491

 Score =  734 bits (1895), Expect = 0.0
 Identities = 363/450 (80%), Positives = 401/450 (89%), Gaps = 2/450 (0%)
 Frame = -2

Query: 1455 LVFRITTASRRKISMPPGSIGLPLIGETFQLISAYKTENPEPFIDTRVSKYGTVFTTHVF 1276
            L+   TT+SRRK  +PPG++GLP IGE  QLI AYKT+NPEPFID+RV+KYGT+FTTHVF
Sbjct: 17   LLHLATTSSRRKTRLPPGTVGLPFIGEXLQLIXAYKTQNPEPFIDSRVAKYGTLFTTHVF 76

Query: 1275 GERTVFSADAETNRFILQNEGRLFESSYPGSISNLVGKHSLLLMRGSLHRRMHSLTMSFA 1096
            GERTVFSAD ETNRFILQNEGRLFESSYPGSI NLVGKHSL  MRGSLH+RMHSLTMSFA
Sbjct: 77   GERTVFSADPETNRFILQNEGRLFESSYPGSILNLVGKHSLSFMRGSLHKRMHSLTMSFA 136

Query: 1095 NSTIIKDHLLVDIDRLVRLNLDSWTGRILLMDEAKKITFELTLKQLLSIDPCEWTENLRK 916
            NSTIIKDHLLVDIDRLVRLNLDSWTGR+LL++EAKKITFELTLKQLLSI+PCEWTENLRK
Sbjct: 137  NSTIIKDHLLVDIDRLVRLNLDSWTGRVLLLEEAKKITFELTLKQLLSIEPCEWTENLRK 196

Query: 915  EYMLVIEGFFCIPLPFFSLTYLRAIQARKRVTEALNLVVXXXXXXXXRGVKKNDMLAALF 736
             YMLVIEGFFCIP+P FS+TY RAI+AR+RV E L+LVV        +G+KKNDMLAALF
Sbjct: 197  IYMLVIEGFFCIPIPMFSITYRRAIKARERVAEELSLVVRERRRESEKGMKKNDMLAALF 256

Query: 735  DSDGE--GVIFRDDEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPLALAELKEEHDEIR 562
            D  G+  GV   D EIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTP ALA+LKEE DEIR
Sbjct: 257  DGGGDVGGVGLTDAEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPRALAQLKEEQDEIR 316

Query: 561  ARKGTSVALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVQIKGYTIPKGSKVFT 382
            ARK  +  +EW+DYKSMPFTQCV+NETLR+SNIISGVFRRAMTDV IKGY IP+GSKVFT
Sbjct: 317  ARKTDAGTIEWEDYKSMPFTQCVINETLRVSNIISGVFRRAMTDVDIKGYRIPRGSKVFT 376

Query: 381  SLRAVHLGQDNFEDARIFDPWRWQKTSDPANFMPFGGGPRRCPGYELARVALSVFLHHLV 202
            SLRAVHLG +NF DAR+FD WRWQK+S+P NFMPFGGGPR+CPG ELARVALSVFLHHLV
Sbjct: 377  SLRAVHLGHENFNDARVFDLWRWQKSSNPTNFMPFGGGPRKCPGNELARVALSVFLHHLV 436

Query: 201  TRFCWKPAEEDKLVFFPTTRTKKRYPIIVQ 112
            TRF W+PAEEDKL+FFPTTRT+KRYPII +
Sbjct: 437  TRFSWEPAEEDKLMFFPTTRTQKRYPIIXE 466


>gb|AAZ39038.1| cytochrome P450 90A2 [Camellia japonica]
          Length = 484

 Score =  699 bits (1805), Expect = 0.0
 Identities = 347/464 (74%), Positives = 398/464 (85%), Gaps = 5/464 (1%)
 Frame = -2

Query: 1464 IIFLVFRITTASRRKISMPPGSIGLPLIGETFQLISAYKTENPEPFIDTRVSKYGTVFTT 1285
            +IF++ R +  +RR + +PPG++GLP +GET  LISAYK+ENP+PFID RVS++G +FTT
Sbjct: 19   LIFVLLRSSIITRRHLRLPPGTLGLPFLGETLHLISAYKSENPDPFIDDRVSRFGPIFTT 78

Query: 1284 HVFGERTVFSADAETNRFILQNEGRLFESSYPGSISNLVGKHSLLLMRGSLHRRMHSLTM 1105
            HVFGE TVFSAD ETNRFILQNEG+LFESSYPGSISNL+G+HSLLLM+GSLH+RMHSLTM
Sbjct: 79   HVFGEPTVFSADPETNRFILQNEGKLFESSYPGSISNLLGRHSLLLMKGSLHKRMHSLTM 138

Query: 1104 SFANSTIIKDHLLVDIDRLVRLNLDSWTGRILLMDEAKKITFELTLKQLLSIDPCEWTEN 925
            SFANS+II+DHL++DIDRLVRLNL+SWTGR+LLM+EAKKITFELT+KQL+S DPCEWTE+
Sbjct: 139  SFANSSIIRDHLMLDIDRLVRLNLESWTGRVLLMEEAKKITFELTVKQLMSFDPCEWTES 198

Query: 924  LRKEYMLVIEGFFCIPLPFFSLTYLRAIQARKRVTEALNLVV-XXXXXXXXRGVKKNDML 748
            LRKEYMLVIEGFF IPLP FS TY RAIQAR +V EAL+LVV         RG +KNDML
Sbjct: 199  LRKEYMLVIEGFFTIPLPLFSSTYRRAIQARSKVAEALSLVVRERRRESSERGERKNDML 258

Query: 747  AALFDSDGEGVIFRDDEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPLALAELKEEHDE 568
             AL D +G G  F D+EIVDFLV+LLVAGY+TTST MTLAVKFLT+TPLALA+LKEEHDE
Sbjct: 259  GALMDEEGGGGGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHDE 318

Query: 567  IRARKGTSVALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVQIKGYTIPKGSKV 388
            IRARK    ALEW+DYKSMPFTQCVVNETLR++NIISGVFRRA+TDV +KGYTIPKG KV
Sbjct: 319  IRARKDNLEALEWNDYKSMPFTQCVVNETLRVANIISGVFRRAITDVDVKGYTIPKGWKV 378

Query: 387  FTSLRAVHLGQDNFEDARIFDPWRWQKTSDPAN----FMPFGGGPRRCPGYELARVALSV 220
            F SLRAVH+  D+F+DAR F+PWRWQ  S   N    F PFGGGPRRCPG ELARV LSV
Sbjct: 379  FASLRAVHMDHDHFKDARSFNPWRWQTNSGTTNSVNLFTPFGGGPRRCPGAELARVELSV 438

Query: 219  FLHHLVTRFCWKPAEEDKLVFFPTTRTKKRYPIIVQHRNMVG*C 88
            FLHHLVTRF W PAEEDKLVFFPTTRT+KRYPII+Q RN+   C
Sbjct: 439  FLHHLVTRFSWVPAEEDKLVFFPTTRTQKRYPIILQRRNVPTSC 482


>emb|CAD27417.1| cytochrome P450 [Nicotiana tabacum]
          Length = 483

 Score =  679 bits (1751), Expect = 0.0
 Identities = 344/468 (73%), Positives = 384/468 (82%), Gaps = 9/468 (1%)
 Frame = -2

Query: 1464 IIFLVFRITTAS---RRKISMPPGSIGLPLIGETFQLISAYKTENPEPFIDTRVSKYGTV 1294
            I FL+ R   A+   RRK  +PPG++GLP IGET QLISAYKTENPEPFID RVSKYG +
Sbjct: 16   ITFLLLRAAAAAHFRRRKTRLPPGTLGLPFIGETLQLISAYKTENPEPFIDDRVSKYGNI 75

Query: 1293 FTTHVFGERTVFSADAETNRFILQNEGRLFESSYPGSISNLVGKHSLLLMRGSLHRRMHS 1114
            FTTH+FGE TVFS DAETNRFILQNEGR FESSYP S+ NL+GKHSLLLMRGSLH+RMHS
Sbjct: 76   FTTHIFGEPTVFSTDAETNRFILQNEGRPFESSYPSSLQNLLGKHSLLLMRGSLHKRMHS 135

Query: 1113 LTMSFANSTIIKDHLLVDIDRLVRLNLDSWTGRILLMDEAKKITFELTLKQLLSIDPCEW 934
            LTMSFANS+I+KDHLL DIDRLVRLNLDSWTGR+ LM+EAKKITF LT+KQL+S+DPCEW
Sbjct: 136  LTMSFANSSILKDHLLADIDRLVRLNLDSWTGRVFLMEEAKKITFNLTVKQLMSLDPCEW 195

Query: 933  TENLRKEYMLVIEGFFCIPLPFFSLTYLRAIQARKRVTEALNLVVXXXXXXXXRGVK-KN 757
            TE L KEYMLVIEGFF IPLPFFS TY +AIQAR++V EAL LVV         G + KN
Sbjct: 196  TEKLMKEYMLVIEGFFTIPLPFFSSTYRKAIQARRKVAEALGLVVKERRKERGGGERLKN 255

Query: 756  DMLAALFDSDG-EGVIFRDDEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPLALAELKE 580
            DML ALF+ DG EG  F D+ IVDF+++LLVAGY+TTST MTLAVKFLT+TP AL+ LKE
Sbjct: 256  DMLEALFEGDGVEG--FSDEVIVDFMLALLVAGYETTSTIMTLAVKFLTETPHALSLLKE 313

Query: 579  EHDEIRARKGTSVALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVQIKGYTIPK 400
            EH+EIR RKG   +L W+DYKSMPFTQCVVNETLR+ NIISGVFRR MTD+ IKGYTIPK
Sbjct: 314  EHEEIRLRKGDVESLLWEDYKSMPFTQCVVNETLRVGNIISGVFRRTMTDINIKGYTIPK 373

Query: 399  GSKVFTSLRAVHLGQDNFEDARIFDPWRWQ----KTSDPANFMPFGGGPRRCPGYELARV 232
            G KVF   RAVHL  ++F+DAR FDPWRWQ     TS P  F PFGGGPRRCPGYELARV
Sbjct: 374  GWKVFACFRAVHLDHEHFKDARTFDPWRWQSNAGSTSSPNVFTPFGGGPRRCPGYELARV 433

Query: 231  ALSVFLHHLVTRFCWKPAEEDKLVFFPTTRTKKRYPIIVQHRNMVG*C 88
             LSVFLHHLVTR  W PAE DKLVFFPTTR +KRYPIIVQ R++   C
Sbjct: 434  ELSVFLHHLVTRHSWVPAEPDKLVFFPTTRMQKRYPIIVQRRSLFDPC 481