BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00003209
         (1596 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudi...   684   0.0  
gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]      682   0.0  
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P...   598   e-168
ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   542   e-151
ref|XP_003629928.1| Cytochrome P450 [Medicago truncatula] gi|355...   501   e-139

>gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudiana]
            gi|189418962|gb|ACD93722.1| ent-kaurenoic acid
            13-hydroxylase [Stevia rebaudiana]
          Length = 476

 Score =  684 bits (1765), Expect = 0.0
 Identities = 329/452 (72%), Positives = 377/452 (83%), Gaps = 1/452 (0%)
 Frame = -3

Query: 1555 KINLPPGSFGWPFIGESFSLFRAGWAGVPERFISERIEKHGSPMVFKTSLFGDRMAVLCG 1376
            KINLPPGSFGWPF+GE+ +L RAGW   PERF+ ERI+KHGSP+VFKTSLFGDR AVLCG
Sbjct: 27   KINLPPGSFGWPFLGETLALLRAGWDSEPERFVRERIKKHGSPLVFKTSLFGDRFAVLCG 86

Query: 1375 PAGNKFLSGNENKLVAVWWPVTFRKLFGSGLITTRGDEAKWMRKMMLSHLGVGPD-LATH 1199
            PAGNKFL  NENKLVA WWPV  RKLFG  L+T RGDEAKWMRKM+LS+LG  PD  ATH
Sbjct: 87   PAGNKFLFCNENKLVASWWPVPVRKLFGKSLLTIRGDEAKWMRKMLLSYLG--PDAFATH 144

Query: 1198 YSTTMDSVTRRHIQIHWQGKEELNVYKSVKLYTFELACRLFMSLEEPNQIAKLSSHFNIF 1019
            Y+ TMD VTRRHI +HW+GKEE+NV+++VKLY FELACRLFM+L++PN IAKL S FNIF
Sbjct: 145  YAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFELACRLFMNLDDPNHIAKLGSLFNIF 204

Query: 1018 LKGIIQLPLDFPGTRFYSAKKAAAAIRIQLMPIIKEKRMALEEGKVXXXXXXXXXXXXXS 839
            LKGII+LP+D PGTRFYS+KKAAAAIRI+L  +IK +++ L+EGK               
Sbjct: 205  LKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKARKLELKEGKASSSQDLLSHLLTSP 264

Query: 838  DENGKYLTEMEIANTILLLLFAGHDTSTAVITLLMKSLSEHPHVYDKVLREHLEICKTKE 659
            DENG +LTE EI + ILLLLFAGHDTS   ITLLMK+L EH  VYDKVL+E LEI KTKE
Sbjct: 265  DENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKTLGEHSDVYDKVLKEQLEISKTKE 324

Query: 658  AGELLKWEDIQKMKYSWSVISEVMRLYPTIIGPFREALVDIEYAGYTIPKGWKLYCPVPS 479
            A E LKWEDIQKMKYSWSVI EVMRL P +IG +REALVDI+YAGYTIPKGWKL+    S
Sbjct: 325  AWESLKWEDIQKMKYSWSVICEVMRLNPPVIGTYREALVDIDYAGYTIPKGWKLHWSAVS 384

Query: 478  THKDEAHYKDVTRFDPSRFEGAGPSPYTYVPFGGGPRMCLGKEYARLEILVFLHNIVTKF 299
            T +DEA+++DVTRFDPSRFEGAGP+P+T+VPFGGGPRMCLGKE+ARLE+L FLHNIVT F
Sbjct: 385  TQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGPRMCLGKEFARLEVLAFLHNIVTNF 444

Query: 298  KWEMLIPNEKIVYDPMVTPEKGLPIHLHPHQV 203
            KW++LIP+EKI YDPM TP KGLPI LHPHQV
Sbjct: 445  KWDLLIPDEKIEYDPMATPAKGLPIRLHPHQV 476


>gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]
          Length = 476

 Score =  682 bits (1759), Expect = 0.0
 Identities = 327/452 (72%), Positives = 377/452 (83%), Gaps = 1/452 (0%)
 Frame = -3

Query: 1555 KINLPPGSFGWPFIGESFSLFRAGWAGVPERFISERIEKHGSPMVFKTSLFGDRMAVLCG 1376
            KINLPPGSFGWPF+GE+ +L RAGW   PERF+ ERI+KHGSP+VFKTSLFGDR AVLCG
Sbjct: 27   KINLPPGSFGWPFLGETLALLRAGWDSEPERFVRERIKKHGSPLVFKTSLFGDRFAVLCG 86

Query: 1375 PAGNKFLSGNENKLVAVWWPVTFRKLFGSGLITTRGDEAKWMRKMMLSHLGVGPD-LATH 1199
            PAGNKFL  NENKLVA WWPV  RKLFG  L+T RGDEAKWMRKM+LS+LG  PD  ATH
Sbjct: 87   PAGNKFLFCNENKLVASWWPVPVRKLFGKSLLTIRGDEAKWMRKMLLSYLG--PDAFATH 144

Query: 1198 YSTTMDSVTRRHIQIHWQGKEELNVYKSVKLYTFELACRLFMSLEEPNQIAKLSSHFNIF 1019
            Y+ TMD VTRRHI +HW+GKEE+NV+++VKLY FELACRLFM+L++PN IAKL S FNIF
Sbjct: 145  YAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFELACRLFMNLDDPNHIAKLGSLFNIF 204

Query: 1018 LKGIIQLPLDFPGTRFYSAKKAAAAIRIQLMPIIKEKRMALEEGKVXXXXXXXXXXXXXS 839
            LKGII+LP+D PGTRFYS+KKAAAAIRI+L  +IK +++ L+EGK               
Sbjct: 205  LKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKARKLELKEGKPSSSQDLLSHLLTSP 264

Query: 838  DENGKYLTEMEIANTILLLLFAGHDTSTAVITLLMKSLSEHPHVYDKVLREHLEICKTKE 659
            DENG +LTE EI + ILLLLFAGHDTS   ITLLMK+L EH  VYDKVL+E LEI K KE
Sbjct: 265  DENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKALGEHSDVYDKVLKEQLEISKGKE 324

Query: 658  AGELLKWEDIQKMKYSWSVISEVMRLYPTIIGPFREALVDIEYAGYTIPKGWKLYCPVPS 479
            AGELLKWEDIQKMKYSWSV+ EVMRL P +IG +REALVDI+YAGYTIPKGWKL+    S
Sbjct: 325  AGELLKWEDIQKMKYSWSVVCEVMRLNPPVIGAYREALVDIDYAGYTIPKGWKLHWSAVS 384

Query: 478  THKDEAHYKDVTRFDPSRFEGAGPSPYTYVPFGGGPRMCLGKEYARLEILVFLHNIVTKF 299
            T +DEA+++DVTRFDPSRFEGAGP+P+T+VPFGGGPRMCLGKE+ARLE+L FLHNIV  F
Sbjct: 385  TQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGPRMCLGKEFARLEVLAFLHNIVINF 444

Query: 298  KWEMLIPNEKIVYDPMVTPEKGLPIHLHPHQV 203
            KW++LIP+EKI YDP+ TP KGLPI LHPHQV
Sbjct: 445  KWDLLIPDEKIEYDPIATPAKGLPIRLHPHQV 476


>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
            annua]
          Length = 480

 Score =  598 bits (1542), Expect = e-168
 Identities = 291/459 (63%), Positives = 355/459 (77%), Gaps = 2/459 (0%)
 Frame = -3

Query: 1573 RTSGSAKINLPPGSFGWPFIGESFSLFRAGWAGVPERFISERIEKHGSPMVFKTSLFGDR 1394
            R + +   NLPPGSFGWPFIGE+ +  R+   G PERF  ERIEK+GS +VFKTS+ G+R
Sbjct: 24   RHNSTTTKNLPPGSFGWPFIGETLAYIRSKRGGDPERFTKERIEKYGSTLVFKTSVAGER 83

Query: 1393 MAVLCGPAGNKFLSGNENKLVAVWWPVTFRKLFGSGLITTRGDEAKWMRKMMLSHLGVGP 1214
            MAV CGP GNKFL GNENKLVA WWP + R LF   LIT RGDEAKW+RKMM ++LG  P
Sbjct: 84   MAVFCGPEGNKFLFGNENKLVASWWPNSVRILFEKCLITIRGDEAKWLRKMMFAYLG--P 141

Query: 1213 D-LATHYSTTMDSVTRRHIQIHWQGKEELNVYKSVKLYTFELACRLFMSLEEPNQIAKLS 1037
            D L+  Y+ TM+ VTR HIQ HWQGK EL V+++V+ Y FELACRLF+SL++P  +A+L 
Sbjct: 142  DALSNRYTGTMEVVTRLHIQNHWQGKSELKVFETVRPYLFELACRLFLSLDDPKHVAELG 201

Query: 1036 SHFNIFLKGIIQLPLDFPGTRFYSAKKAAAAIRIQLMPIIKEKRMAL-EEGKVXXXXXXX 860
            + FN FLKG+ +LP++ PGTRFY AK+AA AI+ QL+ IIK++R AL +E +        
Sbjct: 202  TLFNTFLKGLTELPINIPGTRFYRAKRAANAIKKQLIVIIKQRRQALKQEDQSSSFEDLL 261

Query: 859  XXXXXXSDENGKYLTEMEIANTILLLLFAGHDTSTAVITLLMKSLSEHPHVYDKVLREHL 680
                  SDENG++L+E EIAN +LLLLFAGHDTS   ITLLMKSL+EHP VYD VL+E L
Sbjct: 262  SHLLVSSDENGRFLSEAEIANNVLLLLFAGHDTSAVSITLLMKSLAEHPQVYDNVLKEQL 321

Query: 679  EICKTKEAGELLKWEDIQKMKYSWSVISEVMRLYPTIIGPFREALVDIEYAGYTIPKGWK 500
             I + K  GE+L WEDIQKM+YSW V+ EVMRL P ++G FREALVD EYAGYTIPKGWK
Sbjct: 322  GILEAKAPGEMLNWEDIQKMRYSWYVVCEVMRLIPPVVGSFREALVDFEYAGYTIPKGWK 381

Query: 499  LYCPVPSTHKDEAHYKDVTRFDPSRFEGAGPSPYTYVPFGGGPRMCLGKEYARLEILVFL 320
            +      THK+E ++ + T+FDPSRFEGAGP+P+TYVPFGGGPRMCLGKE AR+ ILVFL
Sbjct: 382  IIWSAVMTHKEENNFPNATKFDPSRFEGAGPTPFTYVPFGGGPRMCLGKELARVRILVFL 441

Query: 319  HNIVTKFKWEMLIPNEKIVYDPMVTPEKGLPIHLHPHQV 203
            HNI+TKFKW++LIP+EKI YDP+ TP KGLP+ LHPHQV
Sbjct: 442  HNIMTKFKWDLLIPDEKIGYDPLATPVKGLPVRLHPHQV 480


>ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 716B2-like [Vitis
            vinifera]
          Length = 473

 Score =  542 bits (1396), Expect = e-151
 Identities = 266/447 (59%), Positives = 331/447 (74%)
 Frame = -3

Query: 1549 NLPPGSFGWPFIGESFSLFRAGWAGVPERFISERIEKHGSPMVFKTSLFGDRMAVLCGPA 1370
            NLPPGS GWP IGE+    R+G  G P+RFI ER++K+ S  VFKTSL G+ M V CGPA
Sbjct: 30   NLPPGSLGWPRIGETLVFLRSGEDGEPQRFIRERMDKYDSG-VFKTSLLGEPMMVFCGPA 88

Query: 1369 GNKFLSGNENKLVAVWWPVTFRKLFGSGLITTRGDEAKWMRKMMLSHLGVGPDLATHYST 1190
            GNKFL GNENKLV+VWWP + RKLF S L+T  GDEAK MR+M+L+ L   PD    Y  
Sbjct: 89   GNKFLFGNENKLVSVWWPSSVRKLFRSCLVTATGDEAKRMRRMLLTFLN--PDALKRYVE 146

Query: 1189 TMDSVTRRHIQIHWQGKEELNVYKSVKLYTFELACRLFMSLEEPNQIAKLSSHFNIFLKG 1010
            TMD VT+ HI+  W+G EE+ VY  +KLYTFELACRLF S+++P  I+KL++HFN+FLKG
Sbjct: 147  TMDLVTQHHIRTQWEGNEEMQVYPIIKLYTFELACRLFASMDDPGHISKLAAHFNVFLKG 206

Query: 1009 IIQLPLDFPGTRFYSAKKAAAAIRIQLMPIIKEKRMALEEGKVXXXXXXXXXXXXXSDEN 830
            II  P++FPGT+F  A KAA AIR +L  +  E+R+ALEE                +D +
Sbjct: 207  IIDFPINFPGTKFXYATKAANAIREELRVMPMERRVALEEKTASATQDLLSHLLVTADAS 266

Query: 829  GKYLTEMEIANTILLLLFAGHDTSTAVITLLMKSLSEHPHVYDKVLREHLEICKTKEAGE 650
            G++LTEMEI + ILLLLFAGHDT+ + ITLL+K L E P VY KVLRE ++I ++KE GE
Sbjct: 267  GRFLTEMEIIDNILLLLFAGHDTTASAITLLIKYLGELPEVYAKVLREKIDITESKEPGE 326

Query: 649  LLKWEDIQKMKYSWSVISEVMRLYPTIIGPFREALVDIEYAGYTIPKGWKLYCPVPSTHK 470
            LL+W+D QKM+YSW V SEVMRL P   G FREALVD  YAGY IPKGWKLY    ST +
Sbjct: 327  LLQWKDXQKMRYSWHVASEVMRLSPPASGSFREALVDFSYAGYNIPKGWKLYWGTGSTQR 386

Query: 469  DEAHYKDVTRFDPSRFEGAGPSPYTYVPFGGGPRMCLGKEYARLEILVFLHNIVTKFKWE 290
            D A +++   FD SRFEGAGP+P++YVPFGGGPRMCLG+E+ARL+ILVF+HNIV +F W+
Sbjct: 387  DPAFFRNPDNFDASRFEGAGPAPFSYVPFGGGPRMCLGQEFARLQILVFMHNIVKRFTWD 446

Query: 289  MLIPNEKIVYDPMVTPEKGLPIHLHPH 209
            +L P+EKI + PM+ P +GLPI LHPH
Sbjct: 447  LLNPDEKIEHSPMLAPLEGLPIRLHPH 473


>ref|XP_003629928.1| Cytochrome P450 [Medicago truncatula] gi|355523950|gb|AET04404.1|
            Cytochrome P450 [Medicago truncatula]
          Length = 948

 Score =  501 bits (1289), Expect = e-139
 Identities = 245/457 (53%), Positives = 323/457 (70%)
 Frame = -3

Query: 1576 KRTSGSAKINLPPGSFGWPFIGESFSLFRAGWAGVPERFISERIEKHGSPMVFKTSLFGD 1397
            KR     K+NLP G+ G+PF+GESF   +A   G   RFI ER++K+ S  VFKTSLFG+
Sbjct: 24   KRIIKLRKLNLPKGTLGFPFVGESFEFLKANLEGKQIRFIQERMKKYDSK-VFKTSLFGE 82

Query: 1396 RMAVLCGPAGNKFLSGNENKLVAVWWPVTFRKLFGSGLITTRGDEAKWMRKMMLSHLGVG 1217
             +AV CGPAGNKFL  NENK V VWWP + +KL    L+   GDEAK  R++++S L   
Sbjct: 83   NIAVFCGPAGNKFLFSNENKNVQVWWPSSVKKLLRLSLVNKVGDEAKVTRRLLMSFLN-- 140

Query: 1216 PDLATHYSTTMDSVTRRHIQIHWQGKEELNVYKSVKLYTFELACRLFMSLEEPNQIAKLS 1037
            P+   +Y   MD + + HI  HW+GKE++ VY  ++LYTFELAC LF+S+E+P  ++ LS
Sbjct: 141  PETLRNYLPNMDRIAQHHINTHWKGKEQVVVYPIIQLYTFELACCLFLSMEDPIDVSNLS 200

Query: 1036 SHFNIFLKGIIQLPLDFPGTRFYSAKKAAAAIRIQLMPIIKEKRMALEEGKVXXXXXXXX 857
            S+F  FLKGII   ++FPGTRF+ A KAA  IR ++  I+K++++ L+E K         
Sbjct: 201  SYFEEFLKGIIGFSINFPGTRFHKAMKAADEIRKEIKMIMKKRKVDLDEKKASPTQDLLS 260

Query: 856  XXXXXSDENGKYLTEMEIANTILLLLFAGHDTSTAVITLLMKSLSEHPHVYDKVLREHLE 677
                  D +G++L E+EI + ILLLLFAGHDTS +V++L+MK L   P VY++VL+E LE
Sbjct: 261  HLLATPDTSGRFLNEVEIIDNILLLLFAGHDTSRSVLSLVMKYLGNLPQVYEQVLKEQLE 320

Query: 676  ICKTKEAGELLKWEDIQKMKYSWSVISEVMRLYPTIIGPFREALVDIEYAGYTIPKGWKL 497
            I + KEAGELL+WEDIQKMKYSW+V SEV+RL P + G FR+A+ D  YA Y IP GWKL
Sbjct: 321  ISQGKEAGELLQWEDIQKMKYSWNVASEVLRLSPPVGGAFRDAIKDFTYADYNIPSGWKL 380

Query: 496  YCPVPSTHKDEAHYKDVTRFDPSRFEGAGPSPYTYVPFGGGPRMCLGKEYARLEILVFLH 317
            +    +TH D   + +  +FD SRFEG GP+PY+YVPFGGGPRMCLG+E+ARLEILVF+H
Sbjct: 381  HWNTHTTHMDPTLFSNPEKFDASRFEGEGPTPYSYVPFGGGPRMCLGQEFARLEILVFMH 440

Query: 316  NIVTKFKWEMLIPNEKIVYDPMVTPEKGLPIHLHPHQ 206
            NIV +FKW+++ P+EK  YDPM+ PE GLPI L P Q
Sbjct: 441  NIVKRFKWDLVNPDEKFKYDPMLEPENGLPIQLQPSQ 477