BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00001362
         (1598 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P46489.1|MDHP_FLABI RecName: Full=Malate dehydrogenase [NADP]...   732   0.0  
gb|AAA87008.1| NADP-malate dehydrogenase [Flaveria trinervia]         726   0.0  
ref|XP_002265080.1| PREDICTED: malate dehydrogenase [NADP], chlo...   726   0.0  
ref|NP_001234056.1| malate dehydrogenase [Solanum lycopersicum] ...   724   0.0  
pdb|1CIV|A Chain A, Chloroplast Nadp-Dependent Malate Dehydrogen...   716   0.0  

>sp|P46489.1|MDHP_FLABI RecName: Full=Malate dehydrogenase [NADP], chloroplastic; AltName:
            Full=NADP-MDH; Flags: Precursor gi|725262|gb|AAA63907.1|
            NADP-malate dehydrogenase precursor [Flaveria bidentis]
          Length = 453

 Score =  732 bits (1889), Expect = 0.0
 Identities = 377/457 (82%), Positives = 405/457 (88%), Gaps = 21/457 (4%)
 Frame = -2

Query: 1549 MAVAELSP----AYTRTEFRNSHLSY------------VASSTRISYH----RRLSFRHL 1430
            MAV +LSP    + +++E ++S  S             V+SS R+S++    RRL  + L
Sbjct: 1    MAVVKLSPWANYSSSKSEIKSSSSSSSSKSSLSAYVINVSSSPRLSFYNPYPRRLHHQRL 60

Query: 1429 PPTQTTAIRCSVTS-DKIQAPVSIDLKQKPDCFGVFCLTYDLKADDETKSWKKMINVAVS 1253
              +   +IRCSVTS D+IQAP  +  KQKP+CFGVFCLTYDLKA++ETKSWKK+INVAVS
Sbjct: 61   --SSPASIRCSVTSSDQIQAP--LPAKQKPECFGVFCLTYDLKAEEETKSWKKIINVAVS 116

Query: 1252 GAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSFGALEGVAMELEDSLYPLLREVSI 1073
            GAAGMISNHLLFKLASGEVFGPDQPI+LKLLGSERSF ALEGVAMELEDSLYPLLR+VSI
Sbjct: 117  GAAGMISNHLLFKLASGEVFGPDQPISLKLLGSERSFAALEGVAMELEDSLYPLLRQVSI 176

Query: 1072 GIDPYEVFEDAEWALLIGAKPRGPGMERAALLDINGQIFAEQGKALNAVASRNVKVMVVG 893
            GIDPYE+F+DAEWALLIGAKPRGPGMERA LLDINGQIFAEQGKALNAVAS NVKVMVVG
Sbjct: 177  GIDPYEIFQDAEWALLIGAKPRGPGMERADLLDINGQIFAEQGKALNAVASPNVKVMVVG 236

Query: 892  NPCNTNALICMKNAPNIPTKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTT 713
            NPCNTNALIC+KNAPNIP KNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTT
Sbjct: 237  NPCNTNALICLKNAPNIPPKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTT 296

Query: 712  QVPDFLNAKIHGIPVPEVIRDRKWLEEEFTEKVQKRGGDLIKKWGRSSAASTAVSIVDAI 533
            QVPDFLNAKIHGIPV EVIRDRKWLE+EFT  VQ RGG LIKKWGRSSAASTAVSIVDAI
Sbjct: 297  QVPDFLNAKIHGIPVTEVIRDRKWLEDEFTNMVQTRGGVLIKKWGRSSAASTAVSIVDAI 356

Query: 532  RSLVTPTPKGDWFSTGVYTNGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRSRI 353
            RSLVTPTP+GDWFSTGVYTNGNPYGIAEDIVFSMPCRSKGDGDYE VKDVIFDDYL  +I
Sbjct: 357  RSLVTPTPEGDWFSTGVYTNGNPYGIAEDIVFSMPCRSKGDGDYEFVKDVIFDDYLSKKI 416

Query: 352  KKSEDELLAEKKCVAHLTGEGIAVCDLPEDTMLPGEM 242
            KKSEDELLAEKKCVAHLTGEGIAVCDLPEDTMLPGEM
Sbjct: 417  KKSEDELLAEKKCVAHLTGEGIAVCDLPEDTMLPGEM 453


>gb|AAA87008.1| NADP-malate dehydrogenase [Flaveria trinervia]
          Length = 416

 Score =  726 bits (1873), Expect = 0.0
 Identities = 364/415 (87%), Positives = 383/415 (92%), Gaps = 1/415 (0%)
 Frame = -2

Query: 1483 VASSTRISYHRRLSFRHLPPTQTTAIRCSVTS-DKIQAPVSIDLKQKPDCFGVFCLTYDL 1307
            +  + R  +H+RLS          +IRCSVTS D+IQAP  +  KQKP+CFGVFCLTYDL
Sbjct: 11   ITLTPRRLHHQRLS-------SPASIRCSVTSSDQIQAP--LPAKQKPECFGVFCLTYDL 61

Query: 1306 KADDETKSWKKMINVAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSFGALEG 1127
            KA++ETKSWKK+INVAVSGAAGMISNHLLFKLASGEVFGPDQPI+LKLLGSERSF ALEG
Sbjct: 62   KAEEETKSWKKIINVAVSGAAGMISNHLLFKLASGEVFGPDQPISLKLLGSERSFAALEG 121

Query: 1126 VAMELEDSLYPLLREVSIGIDPYEVFEDAEWALLIGAKPRGPGMERAALLDINGQIFAEQ 947
            VAMELEDSLYPLLR+VSIGIDPYE+F+DAEWALLIGAKPRGPGMERA LLDINGQIFAEQ
Sbjct: 122  VAMELEDSLYPLLRQVSIGIDPYEIFQDAEWALLIGAKPRGPGMERADLLDINGQIFAEQ 181

Query: 946  GKALNAVASRNVKVMVVGNPCNTNALICMKNAPNIPTKNFHALTRLDENRAKCQLALKAG 767
            GKALNAVAS NVKVMVVGNPCNTNALIC+KNAPNIP KNFHALTRLDENRAKCQLALKAG
Sbjct: 182  GKALNAVASPNVKVMVVGNPCNTNALICLKNAPNIPPKNFHALTRLDENRAKCQLALKAG 241

Query: 766  VFYDKVSNVTIWGNHSTTQVPDFLNAKIHGIPVPEVIRDRKWLEEEFTEKVQKRGGDLIK 587
            VFYDKVSNVTIWGNHSTTQVPDFLNAKIHGIPV EVIRDRKWLE+EFT  VQ RGG LIK
Sbjct: 242  VFYDKVSNVTIWGNHSTTQVPDFLNAKIHGIPVTEVIRDRKWLEDEFTNMVQTRGGVLIK 301

Query: 586  KWGRSSAASTAVSIVDAIRSLVTPTPKGDWFSTGVYTNGNPYGIAEDIVFSMPCRSKGDG 407
            KWGRSSAASTAVSIVDAIRSLVTPTP+GDWFSTGVYTNGNPYGIAEDIVFSMPCRSKGDG
Sbjct: 302  KWGRSSAASTAVSIVDAIRSLVTPTPEGDWFSTGVYTNGNPYGIAEDIVFSMPCRSKGDG 361

Query: 406  DYELVKDVIFDDYLRSRIKKSEDELLAEKKCVAHLTGEGIAVCDLPEDTMLPGEM 242
            DYE VKDVIFDDYL  +IKKSEDELLAEKKCVAHLTGEGIAVCDLPEDTMLPGEM
Sbjct: 362  DYEFVKDVIFDDYLSKKIKKSEDELLAEKKCVAHLTGEGIAVCDLPEDTMLPGEM 416


>ref|XP_002265080.1| PREDICTED: malate dehydrogenase [NADP], chloroplastic [Vitis
            vinifera] gi|296085946|emb|CBI31387.3| unnamed protein
            product [Vitis vinifera]
          Length = 437

 Score =  726 bits (1873), Expect = 0.0
 Identities = 364/441 (82%), Positives = 398/441 (90%), Gaps = 5/441 (1%)
 Frame = -2

Query: 1549 MAVAELSPAYTRTEFR--NSHLSYVASSTRISYHRRLSFRHLPPTQTTAIRCSVTSDKIQ 1376
            MA+A LSP+Y  T+    +S LS++  ST +S+HRR S R LP T+   I CSV S+  +
Sbjct: 1    MALAYLSPSYANTQLHLHSSQLSFL--STHLSFHRRHSLRPLPRTRNARISCSVASN--E 56

Query: 1375 APVSI---DLKQKPDCFGVFCLTYDLKADDETKSWKKMINVAVSGAAGMISNHLLFKLAS 1205
            APV++   D K KP+CFGVFC TYDLKA++ETKSWKK+IN+AVSGAAGMISNHLLFKLA+
Sbjct: 57   APVAVQAQDQKIKPECFGVFCTTYDLKAEEETKSWKKLINIAVSGAAGMISNHLLFKLAA 116

Query: 1204 GEVFGPDQPIALKLLGSERSFGALEGVAMELEDSLYPLLREVSIGIDPYEVFEDAEWALL 1025
            GEVFGPDQPIA KLLGSERSF ALEGVAMELEDSL+PLLREVSIGIDPYEVF+D EWALL
Sbjct: 117  GEVFGPDQPIAFKLLGSERSFQALEGVAMELEDSLFPLLREVSIGIDPYEVFQDVEWALL 176

Query: 1024 IGAKPRGPGMERAALLDINGQIFAEQGKALNAVASRNVKVMVVGNPCNTNALICMKNAPN 845
            IGAKPRGPGMERA LLDINGQIFAEQGKALNAVASRNVKVMVVGNPCNTNALICMKNAPN
Sbjct: 177  IGAKPRGPGMERADLLDINGQIFAEQGKALNAVASRNVKVMVVGNPCNTNALICMKNAPN 236

Query: 844  IPTKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNAKIHGIPVP 665
            IP KNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNA+I+G+PV 
Sbjct: 237  IPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNARINGLPVK 296

Query: 664  EVIRDRKWLEEEFTEKVQKRGGDLIKKWGRSSAASTAVSIVDAIRSLVTPTPKGDWFSTG 485
            EVI+D KWLEEEFTEKVQKRGG LI+KWGRSSAASTAVSIVDA++SLVTPTP+GDWFS+G
Sbjct: 297  EVIKDIKWLEEEFTEKVQKRGGVLIQKWGRSSAASTAVSIVDAMKSLVTPTPEGDWFSSG 356

Query: 484  VYTNGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRSRIKKSEDELLAEKKCVAH 305
            VYT GNPYG+AE IVFSMPCRSKGDGDYELVKDVIFD+YL  RI K+E ELLAEK+CVAH
Sbjct: 357  VYTTGNPYGVAEGIVFSMPCRSKGDGDYELVKDVIFDEYLLKRITKTEAELLAEKRCVAH 416

Query: 304  LTGEGIAVCDLPEDTMLPGEM 242
            LTGEGIA CDLPEDTMLPGEM
Sbjct: 417  LTGEGIAFCDLPEDTMLPGEM 437


>ref|NP_001234056.1| malate dehydrogenase [Solanum lycopersicum]
            gi|56562183|emb|CAH60894.1| malate dehydrogenase [Solanum
            lycopersicum]
          Length = 442

 Score =  724 bits (1868), Expect = 0.0
 Identities = 369/444 (83%), Positives = 400/444 (90%), Gaps = 8/444 (1%)
 Frame = -2

Query: 1549 MAVAELSPAYT----RTEFRNSHLSYVASSTRISYHRRLSFRHLPPTQT-TAIRCSVTSD 1385
            MAVAE  P+ +    +  F +S LS V  ST+IS+ RRLSF+ L  T   + I CSVTS 
Sbjct: 1    MAVAEFIPSSSSLTKKASFYSSQLSNV--STKISHQRRLSFKPLLRTHNHSQICCSVTSK 58

Query: 1384 KIQAPVSI---DLKQKPDCFGVFCLTYDLKADDETKSWKKMINVAVSGAAGMISNHLLFK 1214
            + QA   +   D K+K +C+GVFCLTYDLKA++ET SWKKMI V+VSGAAGMI+NHLLFK
Sbjct: 59   EAQATTVVQTDDPKKKSECYGVFCLTYDLKAEEETSSWKKMITVSVSGAAGMIANHLLFK 118

Query: 1213 LASGEVFGPDQPIALKLLGSERSFGALEGVAMELEDSLYPLLREVSIGIDPYEVFEDAEW 1034
            LASGEVFGPDQPIAL+LLGSERS  ALEGVAMELEDSLYPLLREV+IGIDPYEVF+DAEW
Sbjct: 119  LASGEVFGPDQPIALRLLGSERSIQALEGVAMELEDSLYPLLREVNIGIDPYEVFQDAEW 178

Query: 1033 ALLIGAKPRGPGMERAALLDINGQIFAEQGKALNAVASRNVKVMVVGNPCNTNALICMKN 854
            ALLIGAKPRGPGMERA LLDINGQIFAEQGKALNAVASRNVKV+VVGNPCNTNALIC+KN
Sbjct: 179  ALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASRNVKVIVVGNPCNTNALICLKN 238

Query: 853  APNIPTKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNAKIHGI 674
            APNIP KNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNAKI+G+
Sbjct: 239  APNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNAKINGL 298

Query: 673  PVPEVIRDRKWLEEEFTEKVQKRGGDLIKKWGRSSAASTAVSIVDAIRSLVTPTPKGDWF 494
            PV EVI+D KWLEEEFTEKVQKRGG LI+KWGRSSAASTAVSIVDAIRSLVTPTP+GDWF
Sbjct: 299  PVKEVIKDTKWLEEEFTEKVQKRGGVLIQKWGRSSAASTAVSIVDAIRSLVTPTPEGDWF 358

Query: 493  STGVYTNGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRSRIKKSEDELLAEKKC 314
            STGVYTNGNPYGIA+DIVFSMPCRSKGDGDYELVK+VIFDDYL +RIKKSEDELLAEK+C
Sbjct: 359  STGVYTNGNPYGIAQDIVFSMPCRSKGDGDYELVKEVIFDDYLWNRIKKSEDELLAEKRC 418

Query: 313  VAHLTGEGIAVCDLPEDTMLPGEM 242
            VAHLTGEGIAVCDLP DTMLPGEM
Sbjct: 419  VAHLTGEGIAVCDLPGDTMLPGEM 442


>pdb|1CIV|A Chain A, Chloroplast Nadp-Dependent Malate Dehydrogenase From
            Flaveria Bidentis
          Length = 385

 Score =  716 bits (1848), Expect = 0.0
 Identities = 353/384 (91%), Positives = 368/384 (95%)
 Frame = -2

Query: 1393 TSDKIQAPVSIDLKQKPDCFGVFCLTYDLKADDETKSWKKMINVAVSGAAGMISNHLLFK 1214
            +SD+IQAP  +  KQKP+CFGVFCLTYDLKA++ETKSWKK+INVAVSGAAGMISNHLLFK
Sbjct: 4    SSDQIQAP--LPAKQKPECFGVFCLTYDLKAEEETKSWKKIINVAVSGAAGMISNHLLFK 61

Query: 1213 LASGEVFGPDQPIALKLLGSERSFGALEGVAMELEDSLYPLLREVSIGIDPYEVFEDAEW 1034
            LASGEVFGPDQPI+LKLLGSERSF ALEGVAMELEDSLYPLLR+VSIGIDPYE+F+DAEW
Sbjct: 62   LASGEVFGPDQPISLKLLGSERSFAALEGVAMELEDSLYPLLRQVSIGIDPYEIFQDAEW 121

Query: 1033 ALLIGAKPRGPGMERAALLDINGQIFAEQGKALNAVASRNVKVMVVGNPCNTNALICMKN 854
            ALLIGAKPRGPGMERA LLDINGQIFAEQGKALNAVAS NVKVMVVGNPCNTNALIC+KN
Sbjct: 122  ALLIGAKPRGPGMERADLLDINGQIFAEQGKALNAVASPNVKVMVVGNPCNTNALICLKN 181

Query: 853  APNIPTKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNAKIHGI 674
            APNIP KNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNAKIHGI
Sbjct: 182  APNIPPKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNAKIHGI 241

Query: 673  PVPEVIRDRKWLEEEFTEKVQKRGGDLIKKWGRSSAASTAVSIVDAIRSLVTPTPKGDWF 494
            PV EVIRDRKWLE+EFT  VQ RGG LIKKWGRSSAASTAVSIVDAIRSLVTPTP+GDWF
Sbjct: 242  PVTEVIRDRKWLEDEFTNMVQTRGGVLIKKWGRSSAASTAVSIVDAIRSLVTPTPEGDWF 301

Query: 493  STGVYTNGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRSRIKKSEDELLAEKKC 314
            STGVYTNGNPYGIAEDIVFSMPCRSKGDGDYE VKDVIFDDYL  +IKKSEDELLAEKKC
Sbjct: 302  STGVYTNGNPYGIAEDIVFSMPCRSKGDGDYEFVKDVIFDDYLSKKIKKSEDELLAEKKC 361

Query: 313  VAHLTGEGIAVCDLPEDTMLPGEM 242
            VAHLTGEGIAVCDLPEDTMLPGEM
Sbjct: 362  VAHLTGEGIAVCDLPEDTMLPGEM 385