BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00000823
         (832 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAD27390.1| gamma-glutamylcysteine synthetase [Zinnia elegans]    278   4e-89
ref|XP_002273626.1| PREDICTED: glutamate--cysteine ligase, chlor...   220   8e-72
sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase,...   218   5e-71
ref|XP_002298035.1| predicted protein [Populus trichocarpa] gi|2...   216   4e-70
ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast prec...   216   6e-70

>dbj|BAD27390.1| gamma-glutamylcysteine synthetase [Zinnia elegans]
          Length = 523

 Score =  278 bits (711), Expect(2) = 4e-89
 Identities = 140/171 (81%), Positives = 151/171 (88%)
 Frame = -2

Query: 714 MVLMSQASPSHGIRTEILQCKSGYTALYSGANNTKALRHGTSTVAFPWDSSKTTQNMHVD 535
           MVLMSQASPSHGI  EILQ KSGY++L +GA+NT A RH TS VAF  +  K TQ MHVD
Sbjct: 1   MVLMSQASPSHGIHAEILQSKSGYSSLLNGASNTNAFRHQTSKVAFSRNYLKYTQAMHVD 60

Query: 534 AIGVKSKRRSQMIVAASPPTEDAVVASEPLTKEDLVGYLASGCKPKENWRIGTEHEKFGF 355
           A+G   KR +++IVAASPPTEDAVVA+EPLTKEDLVGYLASGCKPKENWRIGTEHEKFGF
Sbjct: 61  AVGGNFKRGNKVIVAASPPTEDAVVATEPLTKEDLVGYLASGCKPKENWRIGTEHEKFGF 120

Query: 354 ELKTLRPMKYEQIADLLNAISERFDWEKVMEGDNIIGLKQGKQSISLEPGG 202
           +LKTLRPM YEQIA LLNAISERFDWEKVMEGDNIIGLKQGKQSISLEPGG
Sbjct: 121 DLKTLRPMTYEQIAHLLNAISERFDWEKVMEGDNIIGLKQGKQSISLEPGG 171



 Score = 77.0 bits (188), Expect(2) = 4e-89
 Identities = 38/46 (82%), Positives = 38/46 (82%)
 Frame = -3

Query: 140 QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMXXXXXXXXFQPKW 3
           QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEM        FQPKW
Sbjct: 172 QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFIGIGFQPKW 217


>ref|XP_002273626.1| PREDICTED: glutamate--cysteine ligase, chloroplastic [Vitis
           vinifera] gi|297742523|emb|CBI34672.3| unnamed protein
           product [Vitis vinifera]
          Length = 526

 Score =  220 bits (561), Expect(2) = 8e-72
 Identities = 107/174 (61%), Positives = 136/174 (78%), Gaps = 3/174 (1%)
 Frame = -2

Query: 714 MVLMSQASPSHGIRTEILQCKSGYTALYSGANNTKALRHGTSTVAFPWDSSKTTQNMHV- 538
           M L+ Q  PS+ +R+E++ CK+G +A+ +  NN +A +   +       S   T+  H+ 
Sbjct: 1   MALVPQTGPSYCMRSEVVHCKTGQSAVVNMTNNMEASKIKETRTWLSSLSRSRTKTKHIL 60

Query: 537 --DAIGVKSKRRSQMIVAASPPTEDAVVASEPLTKEDLVGYLASGCKPKENWRIGTEHEK 364
             D++GV  K+R Q+IVAASPPTEDAVVA+EPLT+EDL+GYLASGCKPKE WRIGTEHEK
Sbjct: 61  SPDSVGVGCKQRHQVIVAASPPTEDAVVATEPLTREDLIGYLASGCKPKEKWRIGTEHEK 120

Query: 363 FGFELKTLRPMKYEQIADLLNAISERFDWEKVMEGDNIIGLKQGKQSISLEPGG 202
           FGFE +TLRPM+YEQI++LL  +SERF W+K+MEGDNIIGLKQGKQSISLEPGG
Sbjct: 121 FGFEFRTLRPMRYEQISELLYGVSERFGWDKIMEGDNIIGLKQGKQSISLEPGG 174



 Score = 77.0 bits (188), Expect(2) = 8e-72
 Identities = 38/46 (82%), Positives = 38/46 (82%)
 Frame = -3

Query: 140 QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMXXXXXXXXFQPKW 3
           QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEM        FQPKW
Sbjct: 175 QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKW 220


>sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase, chloroplastic; AltName:
           Full=Gamma-ECS; Short=GCS; AltName:
           Full=Gamma-glutamylcysteine synthetase; Flags: Precursor
           gi|111380512|gb|ABD98695.2| chloroplast
           gamma-glutamylcysteine synthetase [Nicotiana tabacum]
          Length = 522

 Score =  218 bits (554), Expect(2) = 5e-71
 Identities = 113/171 (66%), Positives = 132/171 (77%)
 Frame = -2

Query: 714 MVLMSQASPSHGIRTEILQCKSGYTALYSGANNTKALRHGTSTVAFPWDSSKTTQNMHVD 535
           M LMSQA  SH I +E ++C SG++++ S     K        ++   +SSK  Q +++D
Sbjct: 1   MALMSQAGSSHCIYSEKMKCISGHSSITSNMEMLKMKDICFGNISSR-NSSKPMQGIYLD 59

Query: 534 AIGVKSKRRSQMIVAASPPTEDAVVASEPLTKEDLVGYLASGCKPKENWRIGTEHEKFGF 355
            +GV+ +R    IVAASPPTEDAVVA+EPLTKEDLV YLASGCK KE WRIGTEHEKFGF
Sbjct: 60  RVGVERRRGRLAIVAASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKFGF 119

Query: 354 ELKTLRPMKYEQIADLLNAISERFDWEKVMEGDNIIGLKQGKQSISLEPGG 202
           E  TLRPMKYEQIA+LLN I+ERFDWEKVMEGDNIIGLKQGKQSISLEPGG
Sbjct: 120 EFGTLRPMKYEQIAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGG 170



 Score = 77.0 bits (188), Expect(2) = 5e-71
 Identities = 38/46 (82%), Positives = 38/46 (82%)
 Frame = -3

Query: 140 QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMXXXXXXXXFQPKW 3
           QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEM        FQPKW
Sbjct: 171 QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKW 216


>ref|XP_002298035.1| predicted protein [Populus trichocarpa] gi|222845293|gb|EEE82840.1|
           predicted protein [Populus trichocarpa]
          Length = 526

 Score =  216 bits (549), Expect(2) = 4e-70
 Identities = 108/174 (62%), Positives = 135/174 (77%), Gaps = 3/174 (1%)
 Frame = -2

Query: 714 MVLMSQASPSHGIRTEILQCKSGYTALYSGANNTKALRHGTSTVAFP---WDSSKTTQNM 544
           M L++QA PS  I  EI +CK+    + + A +T A +   +   F     +SSKT Q +
Sbjct: 1   MTLVAQAGPSSSILKEISRCKAAQNVVIAMARSTDASKLKETPPRFSSLSCNSSKTPQIL 60

Query: 543 HVDAIGVKSKRRSQMIVAASPPTEDAVVASEPLTKEDLVGYLASGCKPKENWRIGTEHEK 364
             +++ V  KR  +++VAASPPTEDAV+A+EPLTKEDL+GYLASGCKPKENWRIGTEHEK
Sbjct: 61  SSESLSVGMKRGHRVVVAASPPTEDAVIATEPLTKEDLIGYLASGCKPKENWRIGTEHEK 120

Query: 363 FGFELKTLRPMKYEQIADLLNAISERFDWEKVMEGDNIIGLKQGKQSISLEPGG 202
           FGFE+ TLRPMKYEQIA LL+ I+ERFDW+++MEGDNIIGL+QGKQSISLEPGG
Sbjct: 121 FGFEIGTLRPMKYEQIAGLLHGIAERFDWDRIMEGDNIIGLQQGKQSISLEPGG 174



 Score = 75.9 bits (185), Expect(2) = 4e-70
 Identities = 37/46 (80%), Positives = 38/46 (82%)
 Frame = -3

Query: 140 QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMXXXXXXXXFQPKW 3
           QFELSGAP+ETLHQTCAEVNSHLYQVKAVAEEM        FQPKW
Sbjct: 175 QFELSGAPVETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKW 220


>ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast precursor, putative
           [Ricinus communis] gi|223549699|gb|EEF51187.1|
           Glutamate--cysteine ligase, chloroplast precursor,
           putative [Ricinus communis]
          Length = 526

 Score =  216 bits (549), Expect(2) = 6e-70
 Identities = 113/174 (64%), Positives = 134/174 (77%), Gaps = 3/174 (1%)
 Frame = -2

Query: 714 MVLMSQASPSHGIRTEILQCKSGYTALYSGANNTKALRHGTSTVAFP---WDSSKTTQNM 544
           M ++SQA PS  + T+I +CK+G  A+    N+ +  +   + V F     +S+K  Q  
Sbjct: 1   MPVISQAGPSCYVPTDITRCKTGQNAVLGVFNSIETSKLKETCVRFSSLSCNSTKIPQLP 60

Query: 543 HVDAIGVKSKRRSQMIVAASPPTEDAVVASEPLTKEDLVGYLASGCKPKENWRIGTEHEK 364
             +   V+S+R +QMIVAASPP EDAV+A+EPLTKEDLVGYLASGCKPKE WRIGTEHEK
Sbjct: 61  SSENSWVRSRRGNQMIVAASPPIEDAVIATEPLTKEDLVGYLASGCKPKEKWRIGTEHEK 120

Query: 363 FGFELKTLRPMKYEQIADLLNAISERFDWEKVMEGDNIIGLKQGKQSISLEPGG 202
           FGFEL TLRPMKYEQIA+LLN I+ERFDWEKVMEGD IIGLKQGKQSISLEPGG
Sbjct: 121 FGFELGTLRPMKYEQIAELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGG 174



 Score = 75.5 bits (184), Expect(2) = 6e-70
 Identities = 37/46 (80%), Positives = 37/46 (80%)
 Frame = -3

Query: 140 QFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMXXXXXXXXFQPKW 3
           QFELSGAPLETLHQTCAEVNSHLYQVKAV EEM        FQPKW
Sbjct: 175 QFELSGAPLETLHQTCAEVNSHLYQVKAVTEEMGIGFLGIGFQPKW 220