BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus24_contig00026352
         (355 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PNX90562.1| heat shock cognate 70 kDa protein 2-like [Trifoli...    95   2e-22
gb|PNX86652.1| heat shock cognate 70 kDa [Trifolium pratense]          95   9e-22
gb|AFK47618.1| unknown [Medicago truncatula]                           91   8e-21
gb|KHN13880.1| Heat shock cognate 70 kDa protein [Glycine soja]        94   5e-20
ref|XP_006603029.1| PREDICTED: heat shock cognate 70 kDa protein...    94   5e-20
ref|XP_003551819.1| PREDICTED: heat shock cognate 70 kDa protein...    94   5e-20
ref|XP_013451705.1| heat shock cognate 70 kDa-like protein [Medi...    93   2e-19
gb|PNX62590.1| heat shock cognate 70 kDa, partial [Trifolium pra...    86   4e-19
ref|XP_013451697.1| heat shock cognate 70 kDa-like protein [Medi...    91   8e-19
dbj|GAU18500.1| hypothetical protein TSUD_90410 [Trifolium subte...    91   1e-18
gb|KHN13882.1| Heat shock cognate 70 kDa protein [Glycine soja]        91   1e-18
gb|KHN13879.1| Heat shock cognate 70 kDa protein [Glycine soja]        90   1e-18
ref|XP_013451703.1| heat shock cognate 70 kDa-like protein [Medi...    88   7e-18
ref|XP_003552685.1| PREDICTED: heat shock cognate 70 kDa protein...    87   2e-17
dbj|GAU42343.1| hypothetical protein TSUD_287770 [Trifolium subt...    86   6e-17
ref|XP_013451693.1| heat shock cognate 70 kDa-like protein [Medi...    84   2e-16
gb|PNX83143.1| heat shock cognate 70 kDa [Trifolium pratense]          79   1e-14
gb|PNX70870.1| heat shock cognate 70 kDa protein 2-like, partial...    72   6e-14
gb|PNX97117.1| heat shock cognate 70 kDa [Trifolium pratense]          71   6e-12
ref|XP_013451717.1| heat shock cognate 70 kDa protein [Medicago ...    69   3e-11

>gb|PNX90562.1| heat shock cognate 70 kDa protein 2-like [Trifolium pratense]
          Length = 153

 Score = 95.1 bits (235), Expect = 2e-22
 Identities = 53/113 (46%), Positives = 69/113 (61%), Gaps = 3/113 (2%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+ KLS+ DKK INE I  VL WLDVNV  EQ D+EYYR +L+ VF+P I+K++ DE + 
Sbjct: 41  ISSKLSDEDKKAINEVIDSVLMWLDVNVIAEQHDFEYYRSLLSNVFDPIIVKMVSDEGDS 100

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALISVP---ITEAFK 26
           +   T+V    A   KK  W  ILAKY  ++  S  T +L+S     I E FK
Sbjct: 101 LQLGTVV--GYAVDNKKKRWPSILAKYAFEIGYSAATSSLVSTASSVIVEIFK 151


>gb|PNX86652.1| heat shock cognate 70 kDa [Trifolium pratense]
          Length = 217

 Score = 95.1 bits (235), Expect = 9e-22
 Identities = 50/104 (48%), Positives = 71/104 (68%), Gaps = 1/104 (0%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDE-DN 179
           I+LKLS  +KK I+EKI LVL WLDVNV  EQ D+E +RR+L++VF+P I+K+I D+  +
Sbjct: 109 ISLKLSSKEKKEIDEKIDLVLMWLDVNVVAEQHDFEKFRRLLSSVFDPIIMKMIKDDVGS 168

Query: 178 GVPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALISV 47
            V   T+V      +KKK HW  +LAKY L++V +  TG +  +
Sbjct: 169 SVQQGTVV-----KNKKKRHWFLLLAKYTLQIVYTAATGDITGI 207


>gb|AFK47618.1| unknown [Medicago truncatula]
          Length = 152

 Score = 90.9 bits (224), Expect = 8e-21
 Identities = 45/103 (43%), Positives = 66/103 (64%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+ K+S  +K NIN    LV +WLDVNV  EQ+D+E+Y  IL++VF+P ++K+I DE + 
Sbjct: 41  ISSKISSKEKANINNATDLVFKWLDVNVVAEQQDFEHYSSILSSVFDPIVIKMIKDEGHC 100

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALISV 47
           V   T+V      +KKKN WL +LAKY  + V +  TG +  +
Sbjct: 101 VQEGTMV--GHPVNKKKNRWLPLLAKYCFETVYAATTGDITGI 141


>gb|KHN13880.1| Heat shock cognate 70 kDa protein [Glycine soja]
          Length = 673

 Score = 94.4 bits (233), Expect = 5e-20
 Identities = 47/101 (46%), Positives = 69/101 (68%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+LKLS  DK+ IN+ +   LEWL+V+V+ E+ED + +R  L++VF+  ++K+I  EDNG
Sbjct: 563 ISLKLSPEDKEKINDAVDRALEWLEVSVDAEKEDVDNFRGNLSSVFDTIMVKMIKGEDNG 622

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALI 53
            PP    V++  +   KN WL ILAK+GL+ V S VTG +I
Sbjct: 623 APPG--AVASSGSKSGKNRWLSILAKFGLQAVYSAVTGDII 661


>ref|XP_006603029.1| PREDICTED: heat shock cognate 70 kDa protein 2-like isoform X2
           [Glycine max]
          Length = 673

 Score = 94.4 bits (233), Expect = 5e-20
 Identities = 47/101 (46%), Positives = 69/101 (68%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+LKLS  DK+ IN+ +   LEWL+V+V+ E+ED + +R  L++VF+  ++K+I  EDNG
Sbjct: 563 ISLKLSPEDKEKINDAVDRALEWLEVSVDAEKEDVDNFRGNLSSVFDTIMVKMIKGEDNG 622

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALI 53
            PP    V++  +   KN WL ILAK+GL+ V S VTG +I
Sbjct: 623 APPG--AVASSGSKSGKNRWLSILAKFGLQAVYSAVTGDII 661


>ref|XP_003551819.1| PREDICTED: heat shock cognate 70 kDa protein 2-like isoform X1
           [Glycine max]
 gb|KRH01609.1| hypothetical protein GLYMA_18G287800 [Glycine max]
          Length = 673

 Score = 94.4 bits (233), Expect = 5e-20
 Identities = 47/101 (46%), Positives = 69/101 (68%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+LKLS  DK+ IN+ +   LEWL+V+V+ E+ED + +R  L++VF+  ++K+I  EDNG
Sbjct: 563 ISLKLSPEDKEKINDAVDRALEWLEVSVDAEKEDVDNFRGNLSSVFDTIMVKMIKGEDNG 622

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALI 53
            PP    V++  +   KN WL ILAK+GL+ V S VTG +I
Sbjct: 623 APPG--AVASSGSKSGKNRWLSILAKFGLQAVYSAVTGDII 661


>ref|XP_013451705.1| heat shock cognate 70 kDa-like protein [Medicago truncatula]
 gb|KEH25733.1| heat shock cognate 70 kDa-like protein [Medicago truncatula]
          Length = 681

 Score = 92.8 bits (229), Expect = 2e-19
 Identities = 47/103 (45%), Positives = 65/103 (63%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           ++ KLS  +K+ IN  + LV  WLD N  VEQ+D+E YR IL++VF+P ILK+I DE +G
Sbjct: 570 VSSKLSSKEKEKINNAVDLVFMWLDANKVVEQQDFECYRSILSSVFDPIILKMIKDEGHG 629

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALISV 47
           V   T+V       KKKN WL +LAKY  + V +  TG +  +
Sbjct: 630 VEEGTMV--GHPVKKKKNRWLPLLAKYCFQTVYAAATGDITGI 670


>gb|PNX62590.1| heat shock cognate 70 kDa, partial [Trifolium pratense]
          Length = 126

 Score = 85.9 bits (211), Expect = 4e-19
 Identities = 45/87 (51%), Positives = 57/87 (65%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+ KLS+ DKK INE I  VL WLDVNV  EQ D+EYYR +L+ VF+P I+K++ DE + 
Sbjct: 41  ISSKLSDEDKKAINEVIDSVLMWLDVNVIAEQHDFEYYRSLLSNVFDPIIVKMVSDEGDS 100

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKY 95
           +   T+V    A   KK  W  ILAKY
Sbjct: 101 LQLGTVV--GYAVDNKKKRWPSILAKY 125


>ref|XP_013451697.1| heat shock cognate 70 kDa-like protein [Medicago truncatula]
 gb|KEH25725.1| heat shock cognate 70 kDa-like protein [Medicago truncatula]
          Length = 679

 Score = 90.9 bits (224), Expect = 8e-19
 Identities = 45/103 (43%), Positives = 66/103 (64%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+ K+S  +K NIN    LV +WLDVNV  EQ+D+E+Y  IL++VF+P ++K+I DE + 
Sbjct: 568 ISSKISSKEKANINNATDLVFKWLDVNVVAEQQDFEHYSSILSSVFDPIVIKMIKDEGHC 627

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALISV 47
           V   T+V      +KKKN WL +LAKY  + V +  TG +  +
Sbjct: 628 VQEGTMV--GHPVNKKKNRWLPLLAKYCFETVYAATTGDITGI 668


>dbj|GAU18500.1| hypothetical protein TSUD_90410 [Trifolium subterraneum]
          Length = 584

 Score = 90.5 bits (223), Expect = 1e-18
 Identities = 48/106 (45%), Positives = 64/106 (60%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I  KLS  DKK IN+ I+LVL W+D+N+  EQ DYE Y RIL+  F+P ++K+I  E   
Sbjct: 474 IRSKLSNEDKKKINDAINLVLMWIDINIVAEQYDYECYARILSNEFDPIMVKMIKHEVID 533

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALISVPIT 38
           V   T     DA +KK  HWL + AKY  ++V S  TG +I +  T
Sbjct: 534 VQQGTY----DANNKKIRHWLSLFAKYAFQIVTSAATGNIIGLTST 575


>gb|KHN13882.1| Heat shock cognate 70 kDa protein [Glycine soja]
          Length = 584

 Score = 90.5 bits (223), Expect = 1e-18
 Identities = 47/101 (46%), Positives = 67/101 (66%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+LKLS  DKKNIN+ I   LEWL+V+++ +Q D++  R  L++VF P I+K+I DEDN 
Sbjct: 474 ISLKLSPEDKKNINDAIDSALEWLEVSMDAKQNDFDNMRSTLSSVFNPVIVKMIKDEDNV 533

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALI 53
            PP T  V++  ++  KN  L ILA + L+ V S  TG +I
Sbjct: 534 APPDT--VASSGSNSVKNGLLSILANFALEAVYSAATGDII 572


>gb|KHN13879.1| Heat shock cognate 70 kDa protein [Glycine soja]
          Length = 676

 Score = 90.1 bits (222), Expect = 1e-18
 Identities = 46/101 (45%), Positives = 67/101 (66%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+LKLS  DK+ IN+ +   LEWL+V V+ E+ED +  R  L++VF+P ++K+I  EDNG
Sbjct: 566 ISLKLSPEDKEKINDAVDRALEWLEVRVDAEKEDVDMIRGTLSSVFDPIMVKMIKGEDNG 625

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALI 53
            PP T  V++  ++  KN  L ILAK+  + V S VTG +I
Sbjct: 626 APPGT--VASSGSNSGKNRLLSILAKFAFQAVCSSVTGDII 664


>ref|XP_013451703.1| heat shock cognate 70 kDa-like protein [Medicago truncatula]
 gb|KEH25731.1| heat shock cognate 70 kDa-like protein [Medicago truncatula]
          Length = 675

 Score = 88.2 bits (217), Expect = 7e-18
 Identities = 46/97 (47%), Positives = 61/97 (62%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+L LS  +K+ IN+ I LV  WLDVNV  EQE +EYY  IL++VF+  I+K I DE +G
Sbjct: 563 ISLMLSSKEKEKINKAIDLVFMWLDVNVVAEQEQFEYYTSILSSVFDAVIVKKINDERHG 622

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVT 65
           V    +V   +A   KKNHWL+ L KY   +V +  T
Sbjct: 623 VQEGIMV--GNAVKNKKNHWLQTLLKYSFIIVYAAAT 657


>ref|XP_003552685.1| PREDICTED: heat shock cognate 70 kDa protein 2-like [Glycine max]
 gb|KRH01610.1| hypothetical protein GLYMA_18G287900 [Glycine max]
          Length = 673

 Score = 87.0 bits (214), Expect = 2e-17
 Identities = 46/101 (45%), Positives = 64/101 (63%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+LKLS  DKKNIN+ I   LEWL+V+++    D++  R  L++VF P I+K+I DEDN 
Sbjct: 563 ISLKLSPEDKKNINDAIDSALEWLEVSMDANPNDFDNMRSTLSSVFNPVIVKMIKDEDNV 622

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALI 53
            PP T  V++  ++  KN  L ILA + L  V S  TG +I
Sbjct: 623 APPDT--VASSGSNSVKNGLLSILANFALDAVYSAATGDII 661


>dbj|GAU42343.1| hypothetical protein TSUD_287770 [Trifolium subterraneum]
          Length = 663

 Score = 85.5 bits (210), Expect = 6e-17
 Identities = 45/91 (49%), Positives = 56/91 (61%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+ KLS  DKK INE I  VL WLDVNV  EQ D+EYYR +L+ VF+P I+K+I DE+N 
Sbjct: 561 ISSKLSSKDKKVINEAIDSVLMWLDVNVVAEQRDFEYYRTLLSNVFDPIIVKMINDEENN 620

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKL 83
           V              KKN W  +LAKY  ++
Sbjct: 621 V------------DIKKNRWSSLLAKYAFRI 639


>ref|XP_013451693.1| heat shock cognate 70 kDa-like protein [Medicago truncatula]
 gb|KEH25721.1| heat shock cognate 70 kDa-like protein [Medicago truncatula]
          Length = 584

 Score = 84.3 bits (207), Expect = 2e-16
 Identities = 44/97 (45%), Positives = 59/97 (60%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+L LS  +K+ IN  + LV  WLDVNV  EQE +EYY  IL++VF+  I+K I DE +G
Sbjct: 472 ISLMLSSKEKEKINNALDLVFTWLDVNVIAEQEQFEYYTSILSSVFDAVIVKKINDERHG 531

Query: 175 VPPSTLVVSADATSKKKNHWLKILAKYGLKLVISGVT 65
           V    +V   +A   KKNHW + L KY   +V +  T
Sbjct: 532 VQEGIMV--GNAVKNKKNHWKQTLLKYSFIIVHAAAT 566


>gb|PNX83143.1| heat shock cognate 70 kDa [Trifolium pratense]
          Length = 570

 Score = 79.0 bits (193), Expect = 1e-14
 Identities = 41/99 (41%), Positives = 61/99 (61%)
 Frame = -1

Query: 343 LSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNGVPPS 164
           LS  DKK IN+KI LVL WL+VN   E+ D+E++R IL++VF+P ILK++ +E++     
Sbjct: 464 LSSKDKKEINKKIDLVLLWLEVNDTAEKHDFEHFRSILSSVFDPIILKMMTEENDA---Q 520

Query: 163 TLVVSADATSKKKNHWLKILAKYGLKLVISGVTGALISV 47
           T  V       KK+ W+ +LA     +V S  TG +I +
Sbjct: 521 TGTVVGYPVKNKKSRWISLLANXAFNVVYSTATGNIIGL 559


>gb|PNX70870.1| heat shock cognate 70 kDa protein 2-like, partial [Trifolium
           pratense]
          Length = 103

 Score = 72.0 bits (175), Expect = 6e-14
 Identities = 34/61 (55%), Positives = 45/61 (73%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I+ KLS+ DKK INE I  VL WLDVNV  EQ D+EYYR +L+ VF+P I+K++ DE + 
Sbjct: 41  ISSKLSDEDKKAINEVIDSVLMWLDVNVIAEQHDFEYYRSLLSNVFDPIIVKMVSDEGDS 100

Query: 175 V 173
           +
Sbjct: 101 L 101


>gb|PNX97117.1| heat shock cognate 70 kDa [Trifolium pratense]
          Length = 638

 Score = 71.2 bits (173), Expect = 6e-12
 Identities = 42/72 (58%), Positives = 47/72 (65%), Gaps = 2/72 (2%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDE--D 182
           I+ KLS  DKK INE I LVL WLDVNV  EQ D+EYYR IL+ VF+P I K+I DE  D
Sbjct: 561 ISSKLSLKDKKVINEAIDLVLMWLDVNVVAEQCDFEYYRTILSNVFDPIIEKMINDEELD 620

Query: 181 NGVPPSTLVVSA 146
                ST   SA
Sbjct: 621 ERFRSSTAATSA 632


>ref|XP_013451717.1| heat shock cognate 70 kDa protein [Medicago truncatula]
 gb|KEH25745.1| heat shock cognate 70 kDa protein [Medicago truncatula]
          Length = 651

 Score = 69.3 bits (168), Expect = 3e-11
 Identities = 33/61 (54%), Positives = 45/61 (73%)
 Frame = -1

Query: 355 ITLKLSENDKKNINEKIHLVLEWLDVNVNVEQEDYEYYRRILTTVFEPPILKIIGDEDNG 176
           I  KLS  +K+NIN    LV +WLDVNV  EQ+D+E YR IL++VF+P ++K+I DE +G
Sbjct: 568 IISKLSLKEKENINNATDLVFKWLDVNVVAEQQDFECYRSILSSVFDPIVIKMIKDEGHG 627

Query: 175 V 173
           V
Sbjct: 628 V 628