BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus24_contig00021732
         (826 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|GAU44241.1| hypothetical protein TSUD_139340 [Trifolium subt...   396   e-135
ref|XP_013451535.1| cationic amino acid transporter 2, vacuolar ...   397   e-133
gb|KOM56078.1| hypothetical protein LR48_Vigan10g197000 [Vigna a...   382   e-131
ref|XP_013451533.1| cationic amino acid transporter 2, vacuolar ...   393   e-131
ref|XP_007151767.1| hypothetical protein PHAVU_004G0731001g, par...   380   e-129
ref|XP_020206315.1| cationic amino acid transporter 2, vacuolar-...   389   e-129
ref|XP_003525716.1| PREDICTED: cationic amino acid transporter 2...   385   e-127
ref|XP_004489656.1| PREDICTED: cationic amino acid transporter 2...   384   e-127
ref|XP_017440134.1| PREDICTED: cationic amino acid transporter 2...   382   e-127
ref|XP_017440132.1| PREDICTED: cationic amino acid transporter 2...   382   e-126
ref|XP_013451530.1| cationic amino acid transporter 2, vacuolar ...   378   e-125
ref|XP_013451529.1| cationic amino acid transporter 2, vacuolar ...   378   e-125
ref|XP_014524243.1| cationic amino acid transporter 2, vacuolar ...   378   e-125
ref|XP_013451532.1| cationic amino acid transporter 2, vacuolar ...   377   e-124
ref|XP_019453057.1| PREDICTED: cationic amino acid transporter 2...   377   e-124
ref|XP_016179780.1| cationic amino acid transporter 2, vacuolar ...   375   e-124
ref|XP_015946953.1| cationic amino acid transporter 2, vacuolar ...   371   e-122
gb|PNY14246.1| cationic amino acid transporter vacuolar-like, pa...   369   e-122
ref|XP_019442863.1| PREDICTED: cationic amino acid transporter 2...   366   e-120
dbj|GAU23995.1| hypothetical protein TSUD_327960 [Trifolium subt...   366   e-120

>dbj|GAU44241.1| hypothetical protein TSUD_139340 [Trifolium subterraneum]
          Length = 427

 Score =  396 bits (1018), Expect = e-135
 Identities = 206/277 (74%), Positives = 227/277 (81%), Gaps = 11/277 (3%)
 Frame = -2

Query: 798 MGFV--DSKE-SGRN--GEQQSSCSWRFLTRRKKXXXXXXXXXXD------AQLAKELTV 652
           MGFV  DS+E SG N  G+  SSCSW+FLTRRKK          +       QLAKELTV
Sbjct: 1   MGFVGVDSQEGSGSNYGGQGSSSCSWKFLTRRKKVDSNVTNNNNNDNSHHGEQLAKELTV 60

Query: 651 PHLLXXXXXXXXXXXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSA 472
           PHL+           VYVLVGTVAREHAGPALP+SF+VAGLAA LS LCYAELASR PSA
Sbjct: 61  PHLMAIGVGATIGAGVYVLVGTVAREHAGPALPLSFLVAGLAATLSALCYAELASRFPSA 120

Query: 471 GSAYHYAYICVGEGVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQH 292
           GSAYHYAYIC+GEGVAWLIGW+L+LEYGIGGAAVARGITPNL ALIGGM+NLP FLSYQ+
Sbjct: 121 GSAYHYAYICLGEGVAWLIGWSLILEYGIGGAAVARGITPNLAALIGGMDNLPGFLSYQN 180

Query: 291 IPGINIIVDPCAAIMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSG 112
           IPGI+I+VDP AAIMVF+VT +LCTGIKEST+VQSIVTSVN+C LMF+IV GGYLGFKSG
Sbjct: 181 IPGIDIVVDPLAAIMVFIVTWILCTGIKESTMVQSIVTSVNICALMFIIVAGGYLGFKSG 240

Query: 111 WVGYELPTRYFPFGVDGMIAGSATAFFAYTGFDAVAS 1
           W GY+L T YFPFGVDGM+AGSAT FFAY GFDAVAS
Sbjct: 241 WAGYKLSTGYFPFGVDGMVAGSATVFFAYIGFDAVAS 277


>ref|XP_013451535.1| cationic amino acid transporter 2, vacuolar protein [Medicago
           truncatula]
 gb|KEH25563.1| cationic amino acid transporter 2, vacuolar protein [Medicago
           truncatula]
          Length = 575

 Score =  397 bits (1020), Expect = e-133
 Identities = 200/270 (74%), Positives = 224/270 (82%), Gaps = 4/270 (1%)
 Frame = -2

Query: 798 MGFVDS---KESGRN-GEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXX 631
           MGF+     + SG N G Q SSCSW+FLTRRKK            QL KELT+PHL+   
Sbjct: 1   MGFIGDGSHEGSGSNHGGQGSSCSWKFLTRRKKVENSNNSNHG-VQLVKELTIPHLMAIG 59

Query: 630 XXXXXXXXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYA 451
                   VYVLVGTVAREHAGPALP+SFMVAG+AAALS LCYAELASR PSAGSAYHYA
Sbjct: 60  VGATIGAGVYVLVGTVAREHAGPALPLSFMVAGIAAALSALCYAELASRFPSAGSAYHYA 119

Query: 450 YICVGEGVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINII 271
           YIC+GEGVAWLIGW+L+LEYGIGGAAVARGITPNL ALIGG+ NLP FLS QHIPGI+I+
Sbjct: 120 YICLGEGVAWLIGWSLILEYGIGGAAVARGITPNLAALIGGVENLPGFLSNQHIPGIDIV 179

Query: 270 VDPCAAIMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELP 91
           VDP +AIMVF+VT +LCTGIKEST++QSIVTSVN+C LMF+IV GGYLGFK+GW+GY+LP
Sbjct: 180 VDPLSAIMVFIVTWILCTGIKESTMIQSIVTSVNICALMFIIVAGGYLGFKTGWIGYKLP 239

Query: 90  TRYFPFGVDGMIAGSATAFFAYTGFDAVAS 1
           T YFPFGVDGM+AGSATAFFAY GFDAVAS
Sbjct: 240 TGYFPFGVDGMVAGSATAFFAYIGFDAVAS 269


>gb|KOM56078.1| hypothetical protein LR48_Vigan10g197000 [Vigna angularis]
          Length = 269

 Score =  382 bits (981), Expect = e-131
 Identities = 192/264 (72%), Positives = 215/264 (81%)
 Frame = -2

Query: 792 FVDSKESGRNGEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXX 613
           + DSK  G NG +     W F+TRRK+            QLAKELTVPHL+         
Sbjct: 4   WADSKGGGGNGGKNP---WSFVTRRKRVDSAVESNSH-GQLAKELTVPHLMAIGVGATIG 59

Query: 612 XXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGE 433
             VYVLVGTVAREH+GPALP+SF+VAG AAALS LCYAELASRCPSAGSAYHY+YICVGE
Sbjct: 60  AGVYVLVGTVAREHSGPALPLSFLVAGFAAALSALCYAELASRCPSAGSAYHYSYICVGE 119

Query: 432 GVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAA 253
           GVAWLIGWAL+LEY IGGAAVARGITPNL ALIGGM NLPSFLS+  IPG NI+VDPC+A
Sbjct: 120 GVAWLIGWALILEYTIGGAAVARGITPNLAALIGGMENLPSFLSWHSIPGTNIVVDPCSA 179

Query: 252 IMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPF 73
           I+VF+VTGLLC GI+ESTVVQS+VT+VN+  L+FVI+ GGYLGFKSGWVGY+LPT YFPF
Sbjct: 180 IVVFIVTGLLCAGIRESTVVQSVVTTVNIFALIFVIIGGGYLGFKSGWVGYQLPTGYFPF 239

Query: 72  GVDGMIAGSATAFFAYTGFDAVAS 1
           GVDGM+AGSAT FFAY GFDAVAS
Sbjct: 240 GVDGMLAGSATVFFAYIGFDAVAS 263


>ref|XP_013451533.1| cationic amino acid transporter 2, vacuolar protein [Medicago
           truncatula]
 gb|KEH25561.1| cationic amino acid transporter 2, vacuolar protein [Medicago
           truncatula]
          Length = 629

 Score =  393 bits (1010), Expect = e-131
 Identities = 198/268 (73%), Positives = 221/268 (82%), Gaps = 2/268 (0%)
 Frame = -2

Query: 798 MGFVDSKESGRN--GEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXX 625
           MGFVDS+E   +  G Q SSCSWRFLTRRKK            QLAKELT+PHL+     
Sbjct: 1   MGFVDSQEGSGSDYGGQGSSCSWRFLTRRKKVDNSSNSHHG-VQLAKELTIPHLMAIGVG 59

Query: 624 XXXXXXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYI 445
                 VYVLVGTVAREHAGPALP SFMVAG+AAALS LCYAELASR PSAGSAYHYAYI
Sbjct: 60  ATIGAGVYVLVGTVAREHAGPALPFSFMVAGIAAALSALCYAELASRFPSAGSAYHYAYI 119

Query: 444 CVGEGVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVD 265
           C+GEGV+WLIGW+L+LEYG+GGAAVARGITPNL ALIGG++NLP FLSYQ IPGI+I VD
Sbjct: 120 CLGEGVSWLIGWSLILEYGVGGAAVARGITPNLAALIGGVDNLPRFLSYQRIPGIDIDVD 179

Query: 264 PCAAIMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTR 85
           P +A MV +VT +LCTGIKEST +QSIVTSVN+C LMF+IV GGYLGFK+GW+GY+LPT 
Sbjct: 180 PLSAFMVLIVTWILCTGIKESTRIQSIVTSVNICALMFIIVAGGYLGFKTGWIGYKLPTG 239

Query: 84  YFPFGVDGMIAGSATAFFAYTGFDAVAS 1
           YF FGVDGM+AGSATAFFAY GFDAVAS
Sbjct: 240 YFAFGVDGMVAGSATAFFAYIGFDAVAS 267


>ref|XP_007151767.1| hypothetical protein PHAVU_004G0731001g, partial [Phaseolus
           vulgaris]
 gb|ESW23761.1| hypothetical protein PHAVU_004G0731001g, partial [Phaseolus
           vulgaris]
          Length = 347

 Score =  380 bits (977), Expect = e-129
 Identities = 189/264 (71%), Positives = 216/264 (81%)
 Frame = -2

Query: 792 FVDSKESGRNGEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXX 613
           + DSK  G NG + S   W FLTRRK+            QLAKELTVP L+         
Sbjct: 4   WADSKGGGGNGGKNS---WSFLTRRKRVDSAVESNSH-GQLAKELTVPDLMAIGVGATIG 59

Query: 612 XXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGE 433
             VYVLVGTVAREH+GPALP+SF+VAG AAALS LCYAELASRCPSAGSAYHY+YICVGE
Sbjct: 60  AGVYVLVGTVAREHSGPALPLSFLVAGFAAALSALCYAELASRCPSAGSAYHYSYICVGE 119

Query: 432 GVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAA 253
           GVAWLIGWAL+LEY IGGAAVARGITPNL A+IGG  NLPSFLS+  IPG +I++DPC+A
Sbjct: 120 GVAWLIGWALILEYTIGGAAVARGITPNLAAIIGGAENLPSFLSWYTIPGTDIVLDPCSA 179

Query: 252 IMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPF 73
           I++F++TGLLCTGIKESTVVQS+VT+VN+C L+FVI+ GGYLGFKSGWVGY+LPT YFPF
Sbjct: 180 IIIFIITGLLCTGIKESTVVQSVVTTVNICALIFVIIAGGYLGFKSGWVGYKLPTGYFPF 239

Query: 72  GVDGMIAGSATAFFAYTGFDAVAS 1
           GVDGM+AGSAT FFA+ GFDAVAS
Sbjct: 240 GVDGMLAGSATVFFAFIGFDAVAS 263


>ref|XP_020206315.1| cationic amino acid transporter 2, vacuolar-like [Cajanus cajan]
 ref|XP_020206316.1| cationic amino acid transporter 2, vacuolar-like [Cajanus cajan]
 gb|KYP35411.1| High affinity cationic amino acid transporter 1 [Cajanus cajan]
          Length = 642

 Score =  389 bits (998), Expect = e-129
 Identities = 197/264 (74%), Positives = 216/264 (81%)
 Frame = -2

Query: 792 FVDSKESGRNGEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXX 613
           +VDS+E  + G      SWRFLTRRK+            QLAKELTVPHL+         
Sbjct: 4   WVDSEEGRKTG---GLFSWRFLTRRKRVDSAVKSHSQ-GQLAKELTVPHLMAIGVGATIG 59

Query: 612 XXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGE 433
             VYVLVGTVAREH GPALP+SF+VAG AAALS LCYAELASRCPSAGSAYHY+YICVGE
Sbjct: 60  AGVYVLVGTVAREHTGPALPLSFVVAGFAAALSALCYAELASRCPSAGSAYHYSYICVGE 119

Query: 432 GVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAA 253
           GVAWLIGWAL+LEY IGGAAVARGITPNL ALIGG  NLPSFLS   IPGINI+VDPC+A
Sbjct: 120 GVAWLIGWALILEYTIGGAAVARGITPNLAALIGGEENLPSFLSRHLIPGINIVVDPCSA 179

Query: 252 IMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPF 73
           I+VF++TGLLC GIKESTVVQSI+TSVN+C L+FVIV GGYLGFKSGWVGY+LPT YFPF
Sbjct: 180 IIVFIITGLLCVGIKESTVVQSIITSVNICALIFVIVAGGYLGFKSGWVGYKLPTGYFPF 239

Query: 72  GVDGMIAGSATAFFAYTGFDAVAS 1
           GVDGM+AGSAT FFAY GFDAVAS
Sbjct: 240 GVDGMLAGSATVFFAYIGFDAVAS 263


>ref|XP_003525716.1| PREDICTED: cationic amino acid transporter 2, vacuolar-like
           [Glycine max]
 gb|KHN18136.1| Cationic amino acid transporter 2, vacuolar [Glycine soja]
 gb|KRH57867.1| hypothetical protein GLYMA_05G089200 [Glycine max]
          Length = 640

 Score =  385 bits (988), Expect = e-127
 Identities = 191/262 (72%), Positives = 214/262 (81%)
 Frame = -2

Query: 786 DSKESGRNGEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXXXX 607
           DS+E G  G Q   CSW FLTRRK+            QLAKELTVPHL+           
Sbjct: 6   DSEEGGEYGGQ---CSWSFLTRRKRVDSSDKSNSQ-GQLAKELTVPHLMAIGVGATIGAG 61

Query: 606 VYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGEGV 427
           VYVLVGTVAREH+G ALP+SF+VAG AAALS LCYAELASRCPSAGSAYHY+YICVGEGV
Sbjct: 62  VYVLVGTVAREHSGAALPLSFLVAGFAAALSALCYAELASRCPSAGSAYHYSYICVGEGV 121

Query: 426 AWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAAIM 247
           AWLIGWAL+LEY IGGAAVARG+TPNL ALIGG  NLPSFLS   IPGI+++VDPC+AIM
Sbjct: 122 AWLIGWALILEYTIGGAAVARGMTPNLAALIGGGENLPSFLSRHSIPGIDVVVDPCSAIM 181

Query: 246 VFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPFGV 67
           +F++TGLLC GIKEST+VQSI+TS+N+C L+FVI+ GGYLGFKSGWVGYELP  YFPFGV
Sbjct: 182 IFIITGLLCVGIKESTMVQSIITSINICALIFVILAGGYLGFKSGWVGYELPAGYFPFGV 241

Query: 66  DGMIAGSATAFFAYTGFDAVAS 1
           DGM+AGSAT FFAY GFDAVAS
Sbjct: 242 DGMLAGSATVFFAYIGFDAVAS 263


>ref|XP_004489656.1| PREDICTED: cationic amino acid transporter 2, vacuolar-like [Cicer
           arietinum]
          Length = 628

 Score =  384 bits (986), Expect = e-127
 Identities = 186/253 (73%), Positives = 210/253 (83%)
 Frame = -2

Query: 762 GEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXXXXVYVLVGTV 583
           GE  SSCSWRFLTR+KK          + QLAKEL++P L+           VYVLVGTV
Sbjct: 8   GEGSSSCSWRFLTRKKKFDSDVNNNSHNGQLAKELSIPDLMAIGVGATIGAGVYVLVGTV 67

Query: 582 AREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGEGVAWLIGWAL 403
           AREHAGPALP+SFMVAG+AA LS LCYAELAS+CPSAGSAYHYAY+CVGEGVAWLIGW+L
Sbjct: 68  AREHAGPALPLSFMVAGIAATLSALCYAELASKCPSAGSAYHYAYVCVGEGVAWLIGWSL 127

Query: 402 LLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAAIMVFLVTGLL 223
           +LEYG+ GA VARGITPNL ALIGG+NN+P FLSYQHIP I++ VDP AAI+V LVT +L
Sbjct: 128 MLEYGVSGAVVARGITPNLAALIGGVNNIPGFLSYQHIPWIDVDVDPLAAIVVLLVTWVL 187

Query: 222 CTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPFGVDGMIAGSA 43
           CTGIKEST +QSIVTSVN+C LMFVIV GGYLGFK+GW+GY+LPT YFPFGVDG +AGSA
Sbjct: 188 CTGIKESTRIQSIVTSVNICALMFVIVAGGYLGFKTGWIGYQLPTGYFPFGVDGTVAGSA 247

Query: 42  TAFFAYTGFDAVA 4
           TAF AY GFDAVA
Sbjct: 248 TAFLAYIGFDAVA 260


>ref|XP_017440134.1| PREDICTED: cationic amino acid transporter 2, vacuolar isoform X2
           [Vigna angularis]
          Length = 593

 Score =  382 bits (981), Expect = e-127
 Identities = 192/264 (72%), Positives = 215/264 (81%)
 Frame = -2

Query: 792 FVDSKESGRNGEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXX 613
           + DSK  G NG +     W F+TRRK+            QLAKELTVPHL+         
Sbjct: 4   WADSKGGGGNGGKNP---WSFVTRRKRVDSAVESNSH-GQLAKELTVPHLMAIGVGATIG 59

Query: 612 XXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGE 433
             VYVLVGTVAREH+GPALP+SF+VAG AAALS LCYAELASRCPSAGSAYHY+YICVGE
Sbjct: 60  AGVYVLVGTVAREHSGPALPLSFLVAGFAAALSALCYAELASRCPSAGSAYHYSYICVGE 119

Query: 432 GVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAA 253
           GVAWLIGWAL+LEY IGGAAVARGITPNL ALIGGM NLPSFLS+  IPG NI+VDPC+A
Sbjct: 120 GVAWLIGWALILEYTIGGAAVARGITPNLAALIGGMENLPSFLSWHSIPGTNIVVDPCSA 179

Query: 252 IMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPF 73
           I+VF+VTGLLC GI+ESTVVQS+VT+VN+  L+FVI+ GGYLGFKSGWVGY+LPT YFPF
Sbjct: 180 IVVFIVTGLLCAGIRESTVVQSVVTTVNIFALIFVIIGGGYLGFKSGWVGYQLPTGYFPF 239

Query: 72  GVDGMIAGSATAFFAYTGFDAVAS 1
           GVDGM+AGSAT FFAY GFDAVAS
Sbjct: 240 GVDGMLAGSATVFFAYIGFDAVAS 263


>ref|XP_017440132.1| PREDICTED: cationic amino acid transporter 2, vacuolar isoform X1
           [Vigna angularis]
 ref|XP_017440133.1| PREDICTED: cationic amino acid transporter 2, vacuolar isoform X1
           [Vigna angularis]
 dbj|BAU01683.1| hypothetical protein VIGAN_11096600 [Vigna angularis var.
           angularis]
          Length = 629

 Score =  382 bits (981), Expect = e-126
 Identities = 192/264 (72%), Positives = 215/264 (81%)
 Frame = -2

Query: 792 FVDSKESGRNGEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXX 613
           + DSK  G NG +     W F+TRRK+            QLAKELTVPHL+         
Sbjct: 4   WADSKGGGGNGGKNP---WSFVTRRKRVDSAVESNSH-GQLAKELTVPHLMAIGVGATIG 59

Query: 612 XXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGE 433
             VYVLVGTVAREH+GPALP+SF+VAG AAALS LCYAELASRCPSAGSAYHY+YICVGE
Sbjct: 60  AGVYVLVGTVAREHSGPALPLSFLVAGFAAALSALCYAELASRCPSAGSAYHYSYICVGE 119

Query: 432 GVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAA 253
           GVAWLIGWAL+LEY IGGAAVARGITPNL ALIGGM NLPSFLS+  IPG NI+VDPC+A
Sbjct: 120 GVAWLIGWALILEYTIGGAAVARGITPNLAALIGGMENLPSFLSWHSIPGTNIVVDPCSA 179

Query: 252 IMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPF 73
           I+VF+VTGLLC GI+ESTVVQS+VT+VN+  L+FVI+ GGYLGFKSGWVGY+LPT YFPF
Sbjct: 180 IVVFIVTGLLCAGIRESTVVQSVVTTVNIFALIFVIIGGGYLGFKSGWVGYQLPTGYFPF 239

Query: 72  GVDGMIAGSATAFFAYTGFDAVAS 1
           GVDGM+AGSAT FFAY GFDAVAS
Sbjct: 240 GVDGMLAGSATVFFAYIGFDAVAS 263


>ref|XP_013451530.1| cationic amino acid transporter 2, vacuolar protein [Medicago
           truncatula]
 gb|KEH25558.1| cationic amino acid transporter 2, vacuolar protein [Medicago
           truncatula]
          Length = 582

 Score =  378 bits (971), Expect = e-125
 Identities = 189/258 (73%), Positives = 211/258 (81%), Gaps = 4/258 (1%)
 Frame = -2

Query: 762 GEQQSSCSWRFLTRRKKXXXXXXXXXXD----AQLAKELTVPHLLXXXXXXXXXXXVYVL 595
           G Q SSCSWRFLTRRKK                QLAKELTVP L+           VYVL
Sbjct: 9   GGQGSSCSWRFLTRRKKVDNSHVENINKNSHGVQLAKELTVPDLMAIGVGATIGAGVYVL 68

Query: 594 VGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGEGVAWLI 415
           VGTVAREHAGPALP+SFMVAG+AAALS LCYAELASR PSAGSAYHYAYIC+GEGVAWLI
Sbjct: 69  VGTVAREHAGPALPLSFMVAGIAAALSALCYAELASRFPSAGSAYHYAYICLGEGVAWLI 128

Query: 414 GWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAAIMVFLV 235
           GW+L LE  IGGA +ARGITPNL ALIGGM+NLP FLSYQHIPGI+I+VDP AAI+VF+V
Sbjct: 129 GWSLTLEVVIGGAVIARGITPNLAALIGGMDNLPGFLSYQHIPGIDIVVDPLAAIVVFIV 188

Query: 234 TGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPFGVDGMI 55
           T +LCTGIKES ++QSIVTS+N+C LMFVIV GGYLGFK+GW+GYELP  YFPFGV+GM+
Sbjct: 189 TWILCTGIKESAMIQSIVTSMNICALMFVIVAGGYLGFKTGWIGYELPKGYFPFGVNGMV 248

Query: 54  AGSATAFFAYTGFDAVAS 1
           A SATAFFAY GF+A+AS
Sbjct: 249 ASSATAFFAYIGFEAIAS 266


>ref|XP_013451529.1| cationic amino acid transporter 2, vacuolar protein [Medicago
           truncatula]
 gb|KEH25557.1| cationic amino acid transporter 2, vacuolar protein [Medicago
           truncatula]
          Length = 628

 Score =  378 bits (971), Expect = e-125
 Identities = 189/258 (73%), Positives = 211/258 (81%), Gaps = 4/258 (1%)
 Frame = -2

Query: 762 GEQQSSCSWRFLTRRKKXXXXXXXXXXD----AQLAKELTVPHLLXXXXXXXXXXXVYVL 595
           G Q SSCSWRFLTRRKK                QLAKELTVP L+           VYVL
Sbjct: 9   GGQGSSCSWRFLTRRKKVDNSHVENINKNSHGVQLAKELTVPDLMAIGVGATIGAGVYVL 68

Query: 594 VGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGEGVAWLI 415
           VGTVAREHAGPALP+SFMVAG+AAALS LCYAELASR PSAGSAYHYAYIC+GEGVAWLI
Sbjct: 69  VGTVAREHAGPALPLSFMVAGIAAALSALCYAELASRFPSAGSAYHYAYICLGEGVAWLI 128

Query: 414 GWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAAIMVFLV 235
           GW+L LE  IGGA +ARGITPNL ALIGGM+NLP FLSYQHIPGI+I+VDP AAI+VF+V
Sbjct: 129 GWSLTLEVVIGGAVIARGITPNLAALIGGMDNLPGFLSYQHIPGIDIVVDPLAAIVVFIV 188

Query: 234 TGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPFGVDGMI 55
           T +LCTGIKES ++QSIVTS+N+C LMFVIV GGYLGFK+GW+GYELP  YFPFGV+GM+
Sbjct: 189 TWILCTGIKESAMIQSIVTSMNICALMFVIVAGGYLGFKTGWIGYELPKGYFPFGVNGMV 248

Query: 54  AGSATAFFAYTGFDAVAS 1
           A SATAFFAY GF+A+AS
Sbjct: 249 ASSATAFFAYIGFEAIAS 266


>ref|XP_014524243.1| cationic amino acid transporter 2, vacuolar [Vigna radiata var.
           radiata]
          Length = 629

 Score =  378 bits (970), Expect = e-125
 Identities = 191/264 (72%), Positives = 214/264 (81%)
 Frame = -2

Query: 792 FVDSKESGRNGEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXX 613
           + DSK    NG +     W FLTRRK+            QLAKELTVPHL+         
Sbjct: 4   WADSKGVDGNGGKNP---WSFLTRRKRVDSAVESNSH-GQLAKELTVPHLMAIGVGATIG 59

Query: 612 XXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGE 433
             VYVLVGTVAREH+GPALP+SF+VAG AAALS LCYAELASRCPSAGSAYHY+YICVGE
Sbjct: 60  AGVYVLVGTVAREHSGPALPLSFLVAGFAAALSALCYAELASRCPSAGSAYHYSYICVGE 119

Query: 432 GVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAA 253
           GVAWLIGWAL+LEY IGGAAVARGITPNL ALIGGM NLPSFLS+  IPG NI+VDPC+A
Sbjct: 120 GVAWLIGWALILEYTIGGAAVARGITPNLAALIGGMENLPSFLSWHTIPGTNIVVDPCSA 179

Query: 252 IMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPF 73
           I+VF+VTGLLC GI+ESTVVQS+VT+VN+  L+FVI+ GGYLGFKSGWVGY+LPT YFPF
Sbjct: 180 IVVFIVTGLLCAGIRESTVVQSVVTTVNIFALIFVIIGGGYLGFKSGWVGYQLPTGYFPF 239

Query: 72  GVDGMIAGSATAFFAYTGFDAVAS 1
           GVDGM+AGSAT FFA+ GFDAVAS
Sbjct: 240 GVDGMLAGSATVFFAFIGFDAVAS 263


>ref|XP_013451532.1| cationic amino acid transporter 2, vacuolar protein [Medicago
           truncatula]
 gb|KEH25560.1| cationic amino acid transporter 2, vacuolar protein [Medicago
           truncatula]
          Length = 621

 Score =  377 bits (969), Expect = e-124
 Identities = 186/254 (73%), Positives = 209/254 (82%)
 Frame = -2

Query: 762 GEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXXXXVYVLVGTV 583
           GEQ SSCSW FL R+K             QLAKELTVPHL+           +YVLVGTV
Sbjct: 9   GEQGSSCSWSFLRRKK---VNNNSNSHGVQLAKELTVPHLMAIGVGATIGAGLYVLVGTV 65

Query: 582 AREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGEGVAWLIGWAL 403
           AREHAGPALP+SFMVAG+AAA+S LCYAELASR PSAGSAYHYAYIC+GEGVAWLIGW+L
Sbjct: 66  AREHAGPALPLSFMVAGIAAAISALCYAELASRFPSAGSAYHYAYICLGEGVAWLIGWSL 125

Query: 402 LLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAAIMVFLVTGLL 223
            LE  +GGA VARGITPNL ALIGGM+NLP FLSYQHIPGI+I VDP AAIMVF+VT +L
Sbjct: 126 TLETVVGGAVVARGITPNLAALIGGMDNLPGFLSYQHIPGIDIDVDPLAAIMVFIVTWIL 185

Query: 222 CTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPFGVDGMIAGSA 43
           CTGIKES ++QSIVTS+N+C LMFVIV GGYLGFK+GW+GYELP  YFPFG+DGM+A SA
Sbjct: 186 CTGIKESAMIQSIVTSMNICALMFVIVAGGYLGFKTGWIGYELPKGYFPFGIDGMVASSA 245

Query: 42  TAFFAYTGFDAVAS 1
           TAFFAY GF+A+AS
Sbjct: 246 TAFFAYIGFEAIAS 259


>ref|XP_019453057.1| PREDICTED: cationic amino acid transporter 2, vacuolar-like
           [Lupinus angustifolius]
 gb|OIW06464.1| hypothetical protein TanjilG_05235 [Lupinus angustifolius]
          Length = 646

 Score =  377 bits (967), Expect = e-124
 Identities = 193/268 (72%), Positives = 217/268 (80%), Gaps = 5/268 (1%)
 Frame = -2

Query: 789 VDSKESG----RNGEQQSSCSWRFLTRRKKXXXXXXXXXXD-AQLAKELTVPHLLXXXXX 625
           V+SK+ G    RNG Q S   WRF TRRK+            A LAKELTVP+L+     
Sbjct: 5   VESKDGGGGGGRNGGQGS---WRFFTRRKRVDSPTISNNSSQALLAKELTVPNLMAIGVG 61

Query: 624 XXXXXXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYI 445
                 VYVLVGTVAREH+GPAL +SF+VAGLAAALS  CYAELASRCPSAGSAYHY+YI
Sbjct: 62  ATIGAGVYVLVGTVAREHSGPALAISFLVAGLAAALSAFCYAELASRCPSAGSAYHYSYI 121

Query: 444 CVGEGVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVD 265
           CVGEGVAWLIGWAL+LEY IGGAAVARGITPNL AL+GG +NLP FLS Q IPGI+I+VD
Sbjct: 122 CVGEGVAWLIGWALILEYTIGGAAVARGITPNLAALVGGADNLPFFLSRQLIPGIDIVVD 181

Query: 264 PCAAIMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTR 85
           P A I++F++TGLLC GIKEST+VQS+VTSVN+CVL+FVI+ GGYLGFKSGWVGYELPT 
Sbjct: 182 PLAVIIIFIITGLLCVGIKESTMVQSVVTSVNICVLVFVILAGGYLGFKSGWVGYELPTG 241

Query: 84  YFPFGVDGMIAGSATAFFAYTGFDAVAS 1
           YFPFGVDGM+AGSAT FFAY GFDAVAS
Sbjct: 242 YFPFGVDGMLAGSATVFFAYIGFDAVAS 269


>ref|XP_016179780.1| cationic amino acid transporter 2, vacuolar isoform X1 [Arachis
           ipaensis]
 ref|XP_020969275.1| cationic amino acid transporter 2, vacuolar isoform X1 [Arachis
           ipaensis]
          Length = 618

 Score =  375 bits (963), Expect = e-124
 Identities = 187/263 (71%), Positives = 209/263 (79%)
 Frame = -2

Query: 789 VDSKESGRNGEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXXX 610
           VDS++ G NG Q S   W F TRRKK            QLAKELTVP L+          
Sbjct: 5   VDSQDGGENGTQGS---WMFFTRRKKVDSANESSSK-GQLAKELTVPQLMAIGVGATIGA 60

Query: 609 XVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGEG 430
            VYVLVGTVAREH+GP+L +SF+VAG AAALS  CYAELASRCPSAGSAYHY+YICVGEG
Sbjct: 61  GVYVLVGTVAREHSGPSLAISFLVAGFAAALSAFCYAELASRCPSAGSAYHYSYICVGEG 120

Query: 429 VAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAAI 250
           VAWLIGWAL+LEY IGG+AVARGITPNL AL+GG  NLP+FLS  HIPG++I+VDPCAAI
Sbjct: 121 VAWLIGWALILEYTIGGSAVARGITPNLAALVGGAENLPTFLSRHHIPGLDIVVDPCAAI 180

Query: 249 MVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPFG 70
           + F++T LLC GIKES +VQSIVTSVN+C LMFVI+ G YLGFKSGW GYELPT YFPFG
Sbjct: 181 VTFIITALLCFGIKESAMVQSIVTSVNICALMFVILAGSYLGFKSGWAGYELPTGYFPFG 240

Query: 69  VDGMIAGSATAFFAYTGFDAVAS 1
           VDGM+AGSAT FFAY GFDAVAS
Sbjct: 241 VDGMLAGSATVFFAYIGFDAVAS 263


>ref|XP_015946953.1| cationic amino acid transporter 2, vacuolar isoform X1 [Arachis
           duranensis]
 ref|XP_020988899.1| cationic amino acid transporter 2, vacuolar isoform X1 [Arachis
           duranensis]
 ref|XP_020988900.1| cationic amino acid transporter 2, vacuolar isoform X1 [Arachis
           duranensis]
 ref|XP_020988901.1| cationic amino acid transporter 2, vacuolar isoform X1 [Arachis
           duranensis]
          Length = 618

 Score =  371 bits (953), Expect = e-122
 Identities = 185/263 (70%), Positives = 208/263 (79%)
 Frame = -2

Query: 789 VDSKESGRNGEQQSSCSWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXXX 610
           VDS++ G NG Q S   W F TRRKK            QLAKELTVP L+          
Sbjct: 5   VDSQDGGENGTQGS---WMFFTRRKKVDSANESSSK-GQLAKELTVPQLMAIGVGATIGA 60

Query: 609 XVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGEG 430
            VYVLVGTVAREH+GP+L +SF+VAG AAALS  CYAELASRCPSAGSAYHY+Y+CVGEG
Sbjct: 61  GVYVLVGTVAREHSGPSLVISFLVAGFAAALSAFCYAELASRCPSAGSAYHYSYLCVGEG 120

Query: 429 VAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAAI 250
           VAWLIGWAL+LEY I G+AVARGITPNL AL+GG  NLP FLS  HIPG++I+VDPCAAI
Sbjct: 121 VAWLIGWALILEYTISGSAVARGITPNLAALVGGAENLPVFLSRHHIPGLDIVVDPCAAI 180

Query: 249 MVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPFG 70
           + F++T LLC GIKES +VQS+VTSVNVC LMFVI+ GGYLGFKSGW GY+LPT YFPFG
Sbjct: 181 VTFIITALLCFGIKESAMVQSVVTSVNVCALMFVILAGGYLGFKSGWAGYKLPTGYFPFG 240

Query: 69  VDGMIAGSATAFFAYTGFDAVAS 1
           VDGM+AGSAT FFAY GFDAVAS
Sbjct: 241 VDGMLAGSATVFFAYIGFDAVAS 263


>gb|PNY14246.1| cationic amino acid transporter vacuolar-like, partial [Trifolium
           pratense]
          Length = 606

 Score =  369 bits (948), Expect = e-122
 Identities = 183/247 (74%), Positives = 202/247 (81%)
 Frame = -2

Query: 741 SWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXXXXVYVLVGTVAREHAGP 562
           SW+F TRRK+             LAKELTV HL+           VYVLVGTVAREH+GP
Sbjct: 13  SWKFFTRRKQVDSARKNTNTL--LAKELTVSHLIAIGVGSTIGAGVYVLVGTVAREHSGP 70

Query: 561 ALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGEGVAWLIGWALLLEYGIG 382
           AL +SF++AGLAA LS  CYAELA RCPSAGSAYHY+YIC+GEGVAWLIGWAL+LEY IG
Sbjct: 71  ALAISFLIAGLAAGLSAFCYAELACRCPSAGSAYHYSYICLGEGVAWLIGWALVLEYTIG 130

Query: 381 GAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAAIMVFLVTGLLCTGIKES 202
           GAAVARGITPNL  L GG++N+P FL  QHIPGI+I+VDPCAAI+VFLVTGLLC GIKES
Sbjct: 131 GAAVARGITPNLAQLFGGLDNVPFFLLRQHIPGIDIVVDPCAAILVFLVTGLLCLGIKES 190

Query: 201 TVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPFGVDGMIAGSATAFFAYT 22
           TVVQ IVTSVNVC L+FVIV GGY+GFKSGWVGYELPT YFPFGVDGM+AGSAT FFAY 
Sbjct: 191 TVVQGIVTSVNVCALLFVIVAGGYIGFKSGWVGYELPTGYFPFGVDGMLAGSATVFFAYV 250

Query: 21  GFDAVAS 1
           GFDAVAS
Sbjct: 251 GFDAVAS 257


>ref|XP_019442863.1| PREDICTED: cationic amino acid transporter 2, vacuolar-like
           [Lupinus angustifolius]
 gb|OIW12265.1| hypothetical protein TanjilG_06054 [Lupinus angustifolius]
          Length = 618

 Score =  366 bits (940), Expect = e-120
 Identities = 183/264 (69%), Positives = 212/264 (80%), Gaps = 1/264 (0%)
 Frame = -2

Query: 789 VDSKESGRNGEQQSSCSWRFLTRRKKXXXXXXXXXXD-AQLAKELTVPHLLXXXXXXXXX 613
           VDS++ GRNG Q S   W+F TRRK+            AQLAK+LT+ +L+         
Sbjct: 5   VDSEDGGRNGGQGS---WKFFTRRKRVDSTTISNNNSQAQLAKDLTICNLMSIGVGATIG 61

Query: 612 XXVYVLVGTVAREHAGPALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGE 433
             VYVLVGTVARE+ GPAL +SF++AGLAAALS  CYAELASRCPSAGSAYHY+YICVGE
Sbjct: 62  CGVYVLVGTVARENTGPALAISFLIAGLAAALSAFCYAELASRCPSAGSAYHYSYICVGE 121

Query: 432 GVAWLIGWALLLEYGIGGAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAA 253
           GVAWLIGWA++LEY IGG+AVARGI PNL  L+GG N+LP FLS QHIPGI+I+VDP AA
Sbjct: 122 GVAWLIGWAMILEYTIGGSAVARGIAPNLAVLLGGDNSLPFFLSRQHIPGIDIVVDPLAA 181

Query: 252 IMVFLVTGLLCTGIKESTVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPF 73
           IM+F+VTGLLC GIKES + Q+IVTSVN+C L+FVI+ GGYLGFKSGWVGYEL T YFPF
Sbjct: 182 IMIFIVTGLLCVGIKESAMAQNIVTSVNICALVFVIIAGGYLGFKSGWVGYELSTGYFPF 241

Query: 72  GVDGMIAGSATAFFAYTGFDAVAS 1
           GV+GM+AGSAT FFAY GFDAVAS
Sbjct: 242 GVNGMLAGSATVFFAYIGFDAVAS 265


>dbj|GAU23995.1| hypothetical protein TSUD_327960 [Trifolium subterraneum]
          Length = 622

 Score =  366 bits (940), Expect = e-120
 Identities = 182/247 (73%), Positives = 199/247 (80%)
 Frame = -2

Query: 741 SWRFLTRRKKXXXXXXXXXXDAQLAKELTVPHLLXXXXXXXXXXXVYVLVGTVAREHAGP 562
           SW+F TRRK+             LAKELTV HL+           VYVLVGTVAREH+GP
Sbjct: 13  SWKFFTRRKQVDSARKNTKTL--LAKELTVSHLIAIGVGSTIGAGVYVLVGTVAREHSGP 70

Query: 561 ALPVSFMVAGLAAALSGLCYAELASRCPSAGSAYHYAYICVGEGVAWLIGWALLLEYGIG 382
           AL +SF++AGLAA LS  CYAELA RCPSAGSAYHY+YIC+GEGVAWLIGWALLLEY IG
Sbjct: 71  ALAISFLIAGLAAGLSAFCYAELACRCPSAGSAYHYSYICLGEGVAWLIGWALLLEYTIG 130

Query: 381 GAAVARGITPNLGALIGGMNNLPSFLSYQHIPGINIIVDPCAAIMVFLVTGLLCTGIKES 202
            AAVARGITPNL  L GG++NLP FL  QHIPGI+I+VDPCAAI+VFLVTGLLC GIKES
Sbjct: 131 SAAVARGITPNLAQLFGGLDNLPFFLLRQHIPGIDIVVDPCAAILVFLVTGLLCLGIKES 190

Query: 201 TVVQSIVTSVNVCVLMFVIVVGGYLGFKSGWVGYELPTRYFPFGVDGMIAGSATAFFAYT 22
           TVVQ IVTSVNVC L+FVIV GGY+GFKSGWVGYELPT YFPFGVDGM+AGSAT FFAY 
Sbjct: 191 TVVQGIVTSVNVCALLFVIVAGGYIGFKSGWVGYELPTGYFPFGVDGMLAGSATVFFAYV 250

Query: 21  GFDAVAS 1
           GFD V S
Sbjct: 251 GFDGVVS 257