BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus24_contig00020452
         (812 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004488980.1| PREDICTED: alpha-amylase/subtilisin inhibito...   283   2e-93
ref|XP_013464494.1| Kunitz type trypsin inhibitor [Medicago trun...   270   2e-88
dbj|GAU38686.1| hypothetical protein TSUD_54340 [Trifolium subte...   265   3e-86
gb|PNX75086.1| kunitz type trypsin inhibitor [Trifolium pratense]     263   3e-85
ref|NP_001236241.2| kunitz family trypsin and protease inhibitor...   260   2e-84
gb|ACU13608.1| unknown [Glycine max]                                  259   5e-84
gb|KHN19473.1| Alpha-amylase/subtilisin inhibitor [Glycine soja]      258   8e-84
ref|XP_003540516.1| PREDICTED: miraculin-like [Glycine max] >gi|...   254   2e-82
ref|XP_020227907.1| alpha-amylase/subtilisin inhibitor-like [Caj...   253   1e-81
ref|XP_019453538.1| PREDICTED: endogenous alpha-amylase/subtilis...   244   6e-78
ref|XP_019449239.1| PREDICTED: miraculin-like [Lupinus angustifo...   241   7e-77
gb|OIW08195.1| hypothetical protein TanjilG_26484 [Lupinus angus...   235   9e-75
gb|ACS36783.1| unknown [Glycine max]                                  231   6e-73
ref|NP_001237857.2| trypsin inhibitor (Kunitz) family of proteas...   227   2e-71
gb|OIW06052.1| hypothetical protein TanjilG_29808 [Lupinus angus...   226   2e-71
gb|AFK44389.1| unknown [Lotus japonicus]                              225   1e-70
gb|AFK34901.1| unknown [Lotus japonicus]                              225   1e-70
ref|XP_018848596.1| PREDICTED: alpha-amylase/subtilisin inhibito...   224   3e-70
ref|XP_023892637.1| alpha-amylase/subtilisin inhibitor-like [Que...   220   1e-68
ref|XP_003546634.1| PREDICTED: alpha-amylase/subtilisin inhibito...   220   1e-68

>ref|XP_004488980.1| PREDICTED: alpha-amylase/subtilisin inhibitor-like [Cicer
           arietinum]
          Length = 198

 Score =  283 bits (723), Expect = 2e-93
 Identities = 137/200 (68%), Positives = 165/200 (82%), Gaps = 1/200 (0%)
 Frame = +3

Query: 69  MSTKRIE-TSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFT 245
           MST+  + TSLIL+ WLF+  + LAQS+N  VLD KG P+E GKEYYIK A+ D GGRFT
Sbjct: 1   MSTRVAKITSLILVSWLFMAITILAQSENVKVLDTKGHPIERGKEYYIKPAITDSGGRFT 60

Query: 246 LINRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWK 425
           LINRN SCPLYVGQENTDL KGLPV FTPFAK+DKV++V+RDFKV F+A + C QST+WK
Sbjct: 61  LINRNGSCPLYVGQENTDLGKGLPVIFTPFAKEDKVVRVDRDFKVKFSAFSTCVQSTEWK 120

Query: 426 LGQRDLRSRRRLIITGTGNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLR 605
           LG RD +S RR+I+TG+   + +YFRIV TQFEGIYNI+FCPTDVC  C++DCGT+GGLR
Sbjct: 121 LGDRDTKSGRRVIVTGS---SGSYFRIVKTQFEGIYNIRFCPTDVCSFCKYDCGTIGGLR 177

Query: 606 EFFKILLALDGSELPIMFER 665
           E  KILLALDG+ LP++FER
Sbjct: 178 ENGKILLALDGNVLPLVFER 197


>ref|XP_013464494.1| Kunitz type trypsin inhibitor [Medicago truncatula]
 gb|KEH38529.1| Kunitz type trypsin inhibitor [Medicago truncatula]
          Length = 198

 Score =  270 bits (690), Expect = 2e-88
 Identities = 135/201 (67%), Positives = 160/201 (79%), Gaps = 1/201 (0%)
 Frame = +3

Query: 69  MSTKRIE-TSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFT 245
           MST  I+ TSL L++ LF++  TLAQS+N  +LD KG P+E GKEYYIK A+ D GGRFT
Sbjct: 1   MSTTLIKITSLSLMLCLFMSIKTLAQSENEKILDTKGHPLERGKEYYIKPAITDSGGRFT 60

Query: 246 LINRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWK 425
           LI+RN SCPLYVGQENTDL KGLPV FTPFAK+DKVIK +RDFKV F+A++IC QST+WK
Sbjct: 61  LIDRNGSCPLYVGQENTDLGKGLPVIFTPFAKEDKVIKDSRDFKVKFSASSICVQSTEWK 120

Query: 426 LGQRDLRSRRRLIITGTGNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLR 605
           LG RD +S RR+II G+     +YFRIV  +FEG+YNI+FCPTD C  CRFDCG VGGLR
Sbjct: 121 LGDRDTKSGRRVIIAGS---DGSYFRIVKAEFEGVYNIRFCPTDTCSFCRFDCGFVGGLR 177

Query: 606 EFFKILLALDGSELPIMFERA 668
           E  KILLALDG  LP++F RA
Sbjct: 178 ENGKILLALDGGVLPVVFVRA 198


>dbj|GAU38686.1| hypothetical protein TSUD_54340 [Trifolium subterraneum]
          Length = 211

 Score =  265 bits (677), Expect = 3e-86
 Identities = 126/201 (62%), Positives = 161/201 (80%), Gaps = 1/201 (0%)
 Frame = +3

Query: 69  MSTKRIETS-LILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFT 245
           MSTK I+ S L L++WLF+  +TLAQS++  VLD KG P++   +YYIK A+ D GG FT
Sbjct: 1   MSTKFIKISTLSLVLWLFMAITTLAQSEDEQVLDTKGHPLDSRNKYYIKPAITDSGGVFT 60

Query: 246 LINRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWK 425
           LINRN SCPLYVGQENTDL  GLPV FTPFA+ D+V+KVNRDFKV F+A++IC QST+W+
Sbjct: 61  LINRNGSCPLYVGQENTDLDDGLPVIFTPFARQDRVVKVNRDFKVRFSASSICVQSTEWQ 120

Query: 426 LGQRDLRSRRRLIITGTGNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLR 605
           +G RD +S RR++ITG      +YFRIVNTQF+ +YNI+FCPT+VCPLC+FDCGT+G   
Sbjct: 121 VGDRDTKSGRRVVITG---GAGSYFRIVNTQFKSVYNIEFCPTEVCPLCKFDCGTIGRFS 177

Query: 606 EFFKILLALDGSELPIMFERA 668
           E  KILLALDG+ LP++F+R+
Sbjct: 178 ENGKILLALDGNALPVVFQRS 198


>gb|PNX75086.1| kunitz type trypsin inhibitor [Trifolium pratense]
          Length = 215

 Score =  263 bits (671), Expect = 3e-85
 Identities = 120/189 (63%), Positives = 152/189 (80%)
 Frame = +3

Query: 102 LIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTLINRNNSCPLYV 281
           L++WLF+  +TLAQS++  VLD KG P++   +YYIK A+ D GG FTLINRN SCPLYV
Sbjct: 14  LVLWLFMAITTLAQSEDEIVLDTKGHPIDSRNKYYIKPAITDSGGVFTLINRNGSCPLYV 73

Query: 282 GQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKLGQRDLRSRRRL 461
           GQENTDL  GLPV FTPFAK D+ +KVN DFKV F+A++IC QST+WK+G RD +S RR+
Sbjct: 74  GQENTDLDDGLPVIFTPFAKQDRGVKVNSDFKVRFSASSICVQSTEWKVGDRDSKSGRRV 133

Query: 462 IITGTGNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLREFFKILLALDGS 641
           +ITG+ +   +YFRIVNTQF G+YNI+FCPT+ CPLCRFDCGT+G   E  KILLALDG+
Sbjct: 134 VITGSDDGYGSYFRIVNTQFNGVYNIEFCPTEACPLCRFDCGTIGRFVENGKILLALDGT 193

Query: 642 ELPIMFERA 668
            LP++F+R+
Sbjct: 194 ALPVVFQRS 202


>ref|NP_001236241.2| kunitz family trypsin and protease inhibitor protein precursor
           [Glycine max]
 gb|KRH27434.1| hypothetical protein GLYMA_12G234800 [Glycine max]
          Length = 198

 Score =  260 bits (664), Expect = 2e-84
 Identities = 126/200 (63%), Positives = 154/200 (77%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTL 248
           MS + I TSL L+VWL I TS LA+SDNPPVLD +G P+E GK+YYIK A+ D+GGR TL
Sbjct: 1   MSMRSIGTSLSLMVWLVIATSALAKSDNPPVLDTQGNPLEPGKDYYIKPAITDVGGRVTL 60

Query: 249 INRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKL 428
           ++RNN CPLYVGQEN+D  +GLP+ FTPFA++D V+KVNRDFKV F+A +IC Q T W L
Sbjct: 61  LSRNNPCPLYVGQENSDAAEGLPLFFTPFAEEDDVVKVNRDFKVTFSAASICVQGTNWNL 120

Query: 429 GQRDLRSRRRLIITGTGNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLRE 608
            ++D  S RRL I  +G D  +YFRI  T  +G Y I +CPTDVCP CRFDCG VGGLRE
Sbjct: 121 AEKDSESGRRL-IAASGRD--DYFRITETPIKGSYYIGWCPTDVCPFCRFDCGIVGGLRE 177

Query: 609 FFKILLALDGSELPIMFERA 668
             KILLALDG+ LP++FE+A
Sbjct: 178 NGKILLALDGNVLPVVFEKA 197


>gb|ACU13608.1| unknown [Glycine max]
          Length = 198

 Score =  259 bits (661), Expect = 5e-84
 Identities = 125/200 (62%), Positives = 154/200 (77%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTL 248
           MS + I TSL L+VWL I TS LA+SDNPPVLD +G P+E GK+YYIK A+ D+GGR TL
Sbjct: 1   MSMRSIGTSLSLMVWLVIATSALAKSDNPPVLDTQGNPLEPGKDYYIKPAITDVGGRVTL 60

Query: 249 INRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKL 428
           ++RNN CPLYVGQEN+D  +GLP+ FTPFA++D V+KVNRDFK+ F+A +IC Q T W L
Sbjct: 61  LSRNNPCPLYVGQENSDAAEGLPLFFTPFAEEDDVVKVNRDFKLTFSAASICVQGTNWNL 120

Query: 429 GQRDLRSRRRLIITGTGNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLRE 608
            ++D  S RRL I  +G D  +YFRI  T  +G Y I +CPTDVCP CRFDCG VGGLRE
Sbjct: 121 AEKDSESGRRL-IAASGRD--DYFRITETPIKGSYYIGWCPTDVCPFCRFDCGIVGGLRE 177

Query: 609 FFKILLALDGSELPIMFERA 668
             KILLALDG+ LP++FE+A
Sbjct: 178 NGKILLALDGNVLPVVFEKA 197


>gb|KHN19473.1| Alpha-amylase/subtilisin inhibitor [Glycine soja]
          Length = 198

 Score =  258 bits (660), Expect = 8e-84
 Identities = 125/200 (62%), Positives = 154/200 (77%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTL 248
           MS + I TSL L++WL I TS LA+SDNPPVLD +G P+E GK+YYIK A+ D+GGR TL
Sbjct: 1   MSMRSIGTSLSLMLWLVIATSALAKSDNPPVLDTQGNPLEPGKDYYIKPAITDVGGRATL 60

Query: 249 INRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKL 428
           ++RNN CPLYVGQEN+D  +GLP+ FTPFA++D V+KVNRDFKV F+A +IC Q T W L
Sbjct: 61  LSRNNPCPLYVGQENSDAAEGLPLFFTPFAEEDDVVKVNRDFKVTFSAASICVQGTNWNL 120

Query: 429 GQRDLRSRRRLIITGTGNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLRE 608
            ++D  S RRL I  +G D  +YFRI  T  +G Y I +CPTDVCP CRFDCG VGGLRE
Sbjct: 121 AEKDSESGRRL-IAASGRD--DYFRITETPIKGSYYIGWCPTDVCPFCRFDCGIVGGLRE 177

Query: 609 FFKILLALDGSELPIMFERA 668
             KILLALDG+ LP++FE+A
Sbjct: 178 NGKILLALDGNVLPVVFEKA 197


>ref|XP_003540516.1| PREDICTED: miraculin-like [Glycine max]
 gb|KHN19474.1| Miraculin [Glycine soja]
 gb|KRH27433.1| hypothetical protein GLYMA_12G234700 [Glycine max]
          Length = 196

 Score =  254 bits (650), Expect = 2e-82
 Identities = 123/200 (61%), Positives = 153/200 (76%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTL 248
           MSTK IETSL L+VWL I TS +AQSDNPPVLD +G P+E G++YYI  A+ D+GGR T+
Sbjct: 1   MSTKLIETSLSLMVWLVIATSAIAQSDNPPVLDTEGHPLEPGRDYYITPAVTDIGGRATI 60

Query: 249 INRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKL 428
           ++ N +CPL+VGQENT + +   V FTPFAK+D V+KVNRDF+VAF+A T+C Q T W L
Sbjct: 61  VD-NGTCPLFVGQENTFVEESFAVFFTPFAKEDDVVKVNRDFQVAFSAATLCLQGTGWTL 119

Query: 429 GQRDLRSRRRLIITGTGNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLRE 608
           G+RD  S RRLI+ G      +YFRI  TQ +G+YNI +CP DVCP C+FDCG VGGLRE
Sbjct: 120 GERDTESGRRLIVVG---GVGSYFRISETQVKGVYNIGWCPIDVCPFCKFDCGIVGGLRE 176

Query: 609 FFKILLALDGSELPIMFERA 668
             KI LALDG+ LP++FERA
Sbjct: 177 NKKIFLALDGNVLPVVFERA 196


>ref|XP_020227907.1| alpha-amylase/subtilisin inhibitor-like [Cajanus cajan]
 gb|KYP57150.1| Alpha-amylase/subtilisin inhibitor [Cajanus cajan]
          Length = 198

 Score =  253 bits (646), Expect = 1e-81
 Identities = 126/201 (62%), Positives = 157/201 (78%), Gaps = 1/201 (0%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWL-FITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFT 245
           MST+ IET+L L++ L  I T+ LAQS N PVLD KG P+E GK+YYIK A+ D GGR T
Sbjct: 1   MSTRSIETTLTLMLCLAIIATTALAQSTNTPVLDTKGHPLERGKDYYIKPAITDNGGRAT 60

Query: 246 LINRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWK 425
           L++RN+ CPLYVGQEN+D  +GLPV F PF + + +++VNRDFKVAF A+T C Q T W+
Sbjct: 61  LLSRNSPCPLYVGQENSDAEEGLPVFFNPFDEAENIVRVNRDFKVAFNASTTCIQGTGWR 120

Query: 426 LGQRDLRSRRRLIITGTGNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLR 605
           L +RD +S RRLI+  +GND  +YFRIV  Q EGIYNI++CPTDVCP C+FDCGTVG LR
Sbjct: 121 LSERDAKSGRRLIV-ASGND--SYFRIVEAQSEGIYNIRWCPTDVCPYCKFDCGTVGNLR 177

Query: 606 EFFKILLALDGSELPIMFERA 668
           E  KILLALDG+ LP++FERA
Sbjct: 178 ENGKILLALDGNTLPVVFERA 198


>ref|XP_019453538.1| PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like
           [Lupinus angustifolius]
 ref|XP_019453539.1| PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like
           [Lupinus angustifolius]
 ref|XP_019453540.1| PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like
           [Lupinus angustifolius]
 ref|XP_019453541.1| PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like
           [Lupinus angustifolius]
 ref|XP_019453542.1| PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like
           [Lupinus angustifolius]
          Length = 206

 Score =  244 bits (622), Expect = 6e-78
 Identities = 126/207 (60%), Positives = 147/207 (71%), Gaps = 10/207 (4%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTL 248
           MS K I  SL L +WLF+ TSTL+Q+    V+D  G P++ G EYYIK A+ D GGRFTL
Sbjct: 1   MSMKSIYISLTLTLWLFMVTSTLSQT----VIDTNGNPLKRGIEYYIKPAITDNGGRFTL 56

Query: 249 INRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKL 428
           INRNNSCPLYVG ENTD  KGLPV FTPFAK+D V+K+NRDFK++F+A+T C QST WKL
Sbjct: 57  INRNNSCPLYVGLENTDTPKGLPVIFTPFAKEDNVVKLNRDFKISFSASTTCVQSTYWKL 116

Query: 429 GQRDLRSRRRLIITGTG---NDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGG 599
           GQ D +S RRLIITG         NYFR+V  +  GIYNIQ+CPTD+C  CRF CGT  G
Sbjct: 117 GQNDTKSGRRLIITGIDEGRGSYGNYFRVVEGKNAGIYNIQWCPTDLCATCRFICGTASG 176

Query: 600 LRE-------FFKILLALDGSELPIMF 659
           LRE         KILLALDG  LP+ F
Sbjct: 177 LREGGGVKDPNSKILLALDGGVLPVQF 203


>ref|XP_019449239.1| PREDICTED: miraculin-like [Lupinus angustifolius]
          Length = 195

 Score =  241 bits (614), Expect = 7e-77
 Identities = 119/191 (62%), Positives = 141/191 (73%), Gaps = 2/191 (1%)
 Frame = +3

Query: 99  ILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTLINRNNSCPLY 278
           +++V  F+ T  LAQ DN  VLD  G P++ G+EYYIK A+ D GGRFTLINRNNSCPLY
Sbjct: 5   VILVCFFMATLALAQ-DNASVLDTNGNPLKRGEEYYIKPAITDNGGRFTLINRNNSCPLY 63

Query: 279 VGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKLGQRDLRSRRR 458
           VG ENTDL  G  VTFTPFA +D V+K+NRDFKV+F+A T C QSTQW++GQ D    RR
Sbjct: 64  VGLENTDLPDGYAVTFTPFAMEDDVVKINRDFKVSFSAFTTCVQSTQWRVGQNDTSIGRR 123

Query: 459 LIITGT--GNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLREFFKILLAL 632
           LIITG     +  NYF+IV TQ EGIYNI++CPT+VCP CRF CGT G   E  KILLAL
Sbjct: 124 LIITGQDGSREWGNYFKIVETQLEGIYNIKWCPTEVCPTCRFICGTAGAFSENGKILLAL 183

Query: 633 DGSELPIMFER 665
           DGS LP+ F +
Sbjct: 184 DGSVLPVEFHK 194


>gb|OIW08195.1| hypothetical protein TanjilG_26484 [Lupinus angustifolius]
          Length = 184

 Score =  235 bits (599), Expect = 9e-75
 Identities = 117/184 (63%), Positives = 136/184 (73%), Gaps = 2/184 (1%)
 Frame = +3

Query: 120 ITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTLINRNNSCPLYVGQENTD 299
           + T  LAQ DN  VLD  G P++ G+EYYIK A+ D GGRFTLINRNNSCPLYVG ENTD
Sbjct: 1   MATLALAQ-DNASVLDTNGNPLKRGEEYYIKPAITDNGGRFTLINRNNSCPLYVGLENTD 59

Query: 300 LTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKLGQRDLRSRRRLIITGT- 476
           L  G  VTFTPFA +D V+K+NRDFKV+F+A T C QSTQW++GQ D    RRLIITG  
Sbjct: 60  LPDGYAVTFTPFAMEDDVVKINRDFKVSFSAFTTCVQSTQWRVGQNDTSIGRRLIITGQD 119

Query: 477 -GNDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLREFFKILLALDGSELPI 653
              +  NYF+IV TQ EGIYNI++CPT+VCP CRF CGT G   E  KILLALDGS LP+
Sbjct: 120 GSREWGNYFKIVETQLEGIYNIKWCPTEVCPTCRFICGTAGAFSENGKILLALDGSVLPV 179

Query: 654 MFER 665
            F +
Sbjct: 180 EFHK 183


>gb|ACS36783.1| unknown [Glycine max]
          Length = 208

 Score =  231 bits (589), Expect = 6e-73
 Identities = 117/205 (57%), Positives = 144/205 (70%), Gaps = 6/205 (2%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTL 248
           MS + +  SL + VWLF+ T +LAQS+N  V D  G P+E G EYYI+TA+ D GGRFTL
Sbjct: 1   MSMRLLLASLSISVWLFMATLSLAQSNNRYVFDTHGDPLETGDEYYIRTAITDNGGRFTL 60

Query: 249 INRNNSCPLYVGQENTDLTKGLPVTFTPFAK--DDKVIKVNRDFKVAFA-ATTICAQSTQ 419
           INRN SCPLYVG ENTD  +G P+ FTPFA   DD  ++VN D KV     +T C QST+
Sbjct: 61  INRNRSCPLYVGLENTDTPQGYPMKFTPFANKDDDDNLRVNTDLKVTLVQVSTTCVQSTE 120

Query: 420 WKLGQRDLRSRRRLIITGTGN---DTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGT 590
           WKLG+ D RS RRLI+TG  N      NYFRIV T+  GIYNI++CPT+ CP CRF CGT
Sbjct: 121 WKLGENDTRSGRRLIVTGRDNGIQSAGNYFRIVETESVGIYNIRWCPTEACPTCRFICGT 180

Query: 591 VGGLREFFKILLALDGSELPIMFER 665
            G LRE  +IL ALDG+ LP++F++
Sbjct: 181 GGILRENGRILFALDGTTLPVVFQK 205


>ref|NP_001237857.2| trypsin inhibitor (Kunitz) family of protease inhibitors precursor
           [Glycine max]
 gb|KRH44855.1| hypothetical protein GLYMA_08G235300 [Glycine max]
          Length = 208

 Score =  227 bits (579), Expect = 2e-71
 Identities = 116/205 (56%), Positives = 142/205 (69%), Gaps = 6/205 (2%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTL 248
           MS K +  SL + VWLF+ T +LAQS+N  V D  G P+E   EYYI+ A+ D GGRFTL
Sbjct: 1   MSMKLLLASLSISVWLFMATLSLAQSNNRYVFDTHGDPLETDDEYYIRPAITDNGGRFTL 60

Query: 249 INRNNSCPLYVGQENTDLTKGLPVTFTPFAK--DDKVIKVNRDFKVAFA-ATTICAQSTQ 419
           INRN SCPLYVG ENTD  +G P+ FTPFA   DD  ++VN D KV     +T C QST+
Sbjct: 61  INRNRSCPLYVGLENTDTPQGYPMKFTPFANKDDDDNLRVNTDLKVTLVQVSTTCVQSTE 120

Query: 420 WKLGQRDLRSRRRLIITGTGN---DTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGT 590
           WKLG+ D RS RRLI+TG  N      NYFRIV T+  GIYNI++CPT+ CP CRF CGT
Sbjct: 121 WKLGENDTRSGRRLIVTGRDNGIQSAGNYFRIVETESVGIYNIRWCPTEACPTCRFICGT 180

Query: 591 VGGLREFFKILLALDGSELPIMFER 665
            G LRE  +IL ALDG+ LP++F++
Sbjct: 181 GGILRENGRILFALDGTTLPVVFQK 205


>gb|OIW06052.1| hypothetical protein TanjilG_29808 [Lupinus angustifolius]
          Length = 189

 Score =  226 bits (577), Expect = 2e-71
 Identities = 116/190 (61%), Positives = 136/190 (71%), Gaps = 10/190 (5%)
 Frame = +3

Query: 120 ITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTLINRNNSCPLYVGQENTD 299
           + TSTL+Q+    V+D  G P++ G EYYIK A+ D GGRFTLINRNNSCPLYVG ENTD
Sbjct: 1   MVTSTLSQT----VIDTNGNPLKRGIEYYIKPAITDNGGRFTLINRNNSCPLYVGLENTD 56

Query: 300 LTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKLGQRDLRSRRRLIITGTG 479
             KGLPV FTPFAK+D V+K+NRDFK++F+A+T C QST WKLGQ D +S RRLIITG  
Sbjct: 57  TPKGLPVIFTPFAKEDNVVKLNRDFKISFSASTTCVQSTYWKLGQNDTKSGRRLIITGID 116

Query: 480 ---NDTANYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTVGGLRE-------FFKILLA 629
                  NYFR+V  +  GIYNIQ+CPTD+C  CRF CGT  GLRE         KILLA
Sbjct: 117 EGRGSYGNYFRVVEGKNAGIYNIQWCPTDLCATCRFICGTASGLREGGGVKDPNSKILLA 176

Query: 630 LDGSELPIMF 659
           LDG  LP+ F
Sbjct: 177 LDGGVLPVQF 186


>gb|AFK44389.1| unknown [Lotus japonicus]
          Length = 202

 Score =  225 bits (573), Expect = 1e-70
 Identities = 115/203 (56%), Positives = 143/203 (70%), Gaps = 4/203 (1%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTL 248
           M + R+  SLIL VWLF  T +L+Q+ N  VLD  G+P+   KEY+IK A+ D GGRFTL
Sbjct: 1   MLSMRLFMSLILTVWLFTATKSLSQNTN--VLDTNGKPLSRNKEYHIKPAITDNGGRFTL 58

Query: 249 INRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKL 428
           I+RN SCPLYVG ENTDL +G PV FTPFAK DK +K+ +DF V F+A T C QST W +
Sbjct: 59  IDRNQSCPLYVGLENTDLPEGFPVKFTPFAKGDKYVKLGKDFTVVFSAATTCVQSTAWSV 118

Query: 429 GQRDLRSRRRLIITGTG---NDTANYFRIVNTQFE-GIYNIQFCPTDVCPLCRFDCGTVG 596
           G  + +S RRL++TG         +YFRIV  +   GIYNIQ+CPTDVC +CRF CGT G
Sbjct: 119 GSTEAKSGRRLVVTGGDVGERAYGSYFRIVAVERRAGIYNIQWCPTDVCNICRFRCGTAG 178

Query: 597 GLREFFKILLALDGSELPIMFER 665
            LRE  KILLALDG  LP++F++
Sbjct: 179 ILRENGKILLALDGGMLPVVFQK 201


>gb|AFK34901.1| unknown [Lotus japonicus]
          Length = 202

 Score =  225 bits (573), Expect = 1e-70
 Identities = 115/203 (56%), Positives = 143/203 (70%), Gaps = 4/203 (1%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTL 248
           M + R+  SLIL VWLF  T +L+Q+ N  VLD  G+P+   KEY+IK A+ D GGRFTL
Sbjct: 1   MLSMRLFMSLILTVWLFTATKSLSQNTN--VLDTNGKPLSRNKEYHIKPAITDNGGRFTL 58

Query: 249 INRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKL 428
           I+RN SCPLYVG ENTDL +G PV FTPFAK DK +K+ +DF V F+A T C QST W +
Sbjct: 59  IDRNQSCPLYVGLENTDLPEGFPVKFTPFAKGDKYVKLGKDFTVVFSAATTCVQSTAWSV 118

Query: 429 GQRDLRSRRRLIITGTG---NDTANYFRIVNTQFE-GIYNIQFCPTDVCPLCRFDCGTVG 596
           G  + +S RRL++TG         +YFRIV  +   GIYNIQ+CPTDVC +CRF CGT G
Sbjct: 119 GSTEAKSGRRLVVTGGDVGERAYGSYFRIVAVEGRAGIYNIQWCPTDVCNICRFRCGTAG 178

Query: 597 GLREFFKILLALDGSELPIMFER 665
            LRE  KILLALDG  LP++F++
Sbjct: 179 ILRENGKILLALDGGMLPVVFQK 201


>ref|XP_018848596.1| PREDICTED: alpha-amylase/subtilisin inhibitor-like [Juglans regia]
          Length = 207

 Score =  224 bits (571), Expect = 3e-70
 Identities = 112/193 (58%), Positives = 136/193 (70%), Gaps = 7/193 (3%)
 Frame = +3

Query: 108 VWLFITTSTLA---QSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTLINRNNSCPLY 278
           +WL +  S +     S   PVLD +G+P+E G EYYI  A+ D GGRFTLINRN+SCP Y
Sbjct: 13  IWLVLAISAVVAQPSSSPSPVLDSEGRPLERGVEYYINPAITDNGGRFTLINRNDSCPFY 72

Query: 279 VGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWKLGQRDLRSRRR 458
           VGQEN    +G PV FTPFA++D VI+ NRD ++ F+A+TIC QST WKLG+RD  S+RR
Sbjct: 73  VGQENVSGPEGFPVIFTPFAEEDTVIRENRDLRIVFSASTICVQSTAWKLGERDPESQRR 132

Query: 459 LIITGTGN---DTANYFRIVNTQF-EGIYNIQFCPTDVCPLCRFDCGTVGGLREFFKILL 626
           LI+TG       T NYFR+V     + IY I +CPTDVCP CRF+CGT GGL E  K LL
Sbjct: 133 LIVTGEDQGRLSTGNYFRLVKAAVGDNIYEISWCPTDVCPTCRFNCGTAGGLVENGKRLL 192

Query: 627 ALDGSELPIMFER 665
           ALDGS LP+MFER
Sbjct: 193 ALDGSVLPVMFER 205


>ref|XP_023892637.1| alpha-amylase/subtilisin inhibitor-like [Quercus suber]
          Length = 206

 Score =  220 bits (560), Expect = 1e-68
 Identities = 114/204 (55%), Positives = 139/204 (68%), Gaps = 5/204 (2%)
 Frame = +3

Query: 69  MSTKRIETSLILIVWLFITTSTLAQSDNP-PVLDIKGQPVEVGKEYYIKTALLDLGGRFT 245
           M + R+  SL  I+ +   ++    S +P PVLD  G+P++ G EYYI  A+ D GGRFT
Sbjct: 1   MKSMRLIGSLSCIMLVMAISAVAQPSSSPAPVLDSAGRPLQRGVEYYINPAITDSGGRFT 60

Query: 246 LINRNNSCPLYVGQENTDLTKGLPVTFTPFAKDDKVIKVNRDFKVAFAATTICAQSTQWK 425
           LINRN SCP YVGQEN    +GLPV FTPF + + VI+ NRDF+VAF+A TIC QST WK
Sbjct: 61  LINRNGSCPFYVGQENVSGLEGLPVIFTPFVEGETVIRENRDFRVAFSAATICVQSTAWK 120

Query: 426 LGQRDLRSRRRLIITG---TGNDTANYFRIVNTQFEG-IYNIQFCPTDVCPLCRFDCGTV 593
           LG++D  S RRLI+TG   +   TANYFRI      G IY I +CPTDVCP CRFDCGT 
Sbjct: 121 LGEKDPESNRRLIVTGEDQSSRRTANYFRIEKASVGGDIYQISWCPTDVCPTCRFDCGTA 180

Query: 594 GGLREFFKILLALDGSELPIMFER 665
           G L E  K LLALDG+ +P+ FER
Sbjct: 181 GNLVENGKRLLALDGNVIPVTFER 204


>ref|XP_003546634.1| PREDICTED: alpha-amylase/subtilisin inhibitor-like [Glycine max]
 gb|KRH13021.1| hypothetical protein GLYMA_15G211500 [Glycine max]
          Length = 206

 Score =  220 bits (560), Expect = 1e-68
 Identities = 116/204 (56%), Positives = 142/204 (69%), Gaps = 9/204 (4%)
 Frame = +3

Query: 81  RIETSLILIVWLFITTSTLAQSDNPPVLDIKGQPVEVGKEYYIKTALLDLGGRFTLINRN 260
           ++  SL  +VWLF+ TS+LAQ     VLD   + V+   EYYI+ A+ D GGRFTLINRN
Sbjct: 4   KLYASLTFVVWLFMATSSLAQY----VLDTNRERVDSDDEYYIRPAITDNGGRFTLINRN 59

Query: 261 NSCPLYVGQENTDLTKGLPVTFTPFAK-----DDKVIKVNRDFKVAF-AATTICAQSTQW 422
            SCPLYVG ENTD   G PV FTPF++     DD  I+VNRD +VAF   +T C QST+W
Sbjct: 60  RSCPLYVGLENTDTPLGYPVKFTPFSRNNNDDDDDDIRVNRDLRVAFDEVSTTCVQSTEW 119

Query: 423 KLGQRDLRSRRRLIITGTGNDTA---NYFRIVNTQFEGIYNIQFCPTDVCPLCRFDCGTV 593
           ++G+ D RS RRLIITG    T    NYFRIV T+  GIYNIQ+CPT+VCP CRF CGT 
Sbjct: 120 RVGENDTRSGRRLIITGRDETTGSYGNYFRIVETENVGIYNIQWCPTEVCPTCRFICGTG 179

Query: 594 GGLREFFKILLALDGSELPIMFER 665
           G LRE  +IL ALDG+ LP+MF++
Sbjct: 180 GILRENGRILFALDGTPLPVMFQK 203