BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus24_contig00015504
         (302 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arieti...    81   1e-15
dbj|GAU12625.1| hypothetical protein TSUD_121160 [Trifolium subt...    75   2e-13
dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subt...    75   2e-13
sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName:...    73   7e-13
dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subt...    70   6e-12
gb|PNY06816.1| DNA topoisomerase 2-like protein [Trifolium prate...    68   5e-11
ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago tru...    66   2e-10
dbj|GAU30192.1| hypothetical protein TSUD_311430 [Trifolium subt...    64   1e-09
ref|XP_003601827.1| DNA topoisomerase IV subunit A [Medicago tru...    64   2e-09
gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium prate...    62   6e-09
ref|XP_014501355.1| DNA topoisomerase 2 [Vigna radiata var. radi...    59   9e-08
gb|KRH32967.1| hypothetical protein GLYMA_10G090000 [Glycine max]      57   5e-07
ref|XP_014618555.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisom...    57   5e-07
gb|KYP71391.1| DNA topoisomerase 2 [Cajanus cajan]                     55   2e-06
ref|XP_020212372.1| DNA topoisomerase 2 [Cajanus cajan]                55   2e-06
ref|XP_015936924.1| DNA topoisomerase 2 isoform X2 [Arachis dura...    54   5e-06
ref|XP_015936922.1| DNA topoisomerase 2 isoform X1 [Arachis dura...    54   5e-06
ref|XP_017425524.1| PREDICTED: DNA topoisomerase 2 [Vigna angula...    53   7e-06
ref|XP_016170361.1| DNA topoisomerase 2 isoform X2 [Arachis ipae...    53   7e-06
ref|XP_016170360.1| DNA topoisomerase 2 isoform X1 [Arachis ipae...    53   7e-06

>ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
 ref|XP_004517303.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
          Length = 1472

 Score = 81.3 bits (199), Expect = 1e-15
 Identities = 56/122 (45%), Positives = 74/122 (60%), Gaps = 28/122 (22%)
 Frame = +2

Query: 2    GEKTGALETEEPPLPDGKKQNNRKGGA-KKTTTVVLD-TDSENEVSDA-DDYDFEVVVQ- 169
            GEK+ A+ETEEP +P  KK  NR+GG  KK++T+VLD +DS+N+V++  DD DFEV  + 
Sbjct: 1258 GEKSAAMETEEPAVPAAKKPINRRGGVKKKSSTIVLDESDSDNDVNNIDDDDDFEVQQKA 1317

Query: 170  QPAPEAGKMKVG--------------------RNLGTKRKLGQM----MLQPAEGTHITR 277
             PAPEAGK K G                    RN+G K+ +GQ     MLQPAEGT  + 
Sbjct: 1318 APAPEAGKKKGGGRKPAAQNTKKAAAPAAPKKRNVGGKQTMGQKLLTDMLQPAEGTGTSP 1377

Query: 278  EE 283
            E+
Sbjct: 1378 EK 1379


>dbj|GAU12625.1| hypothetical protein TSUD_121160 [Trifolium subterraneum]
          Length = 1315

 Score = 74.7 bits (182), Expect = 2e-13
 Identities = 51/117 (43%), Positives = 69/117 (58%), Gaps = 23/117 (19%)
 Frame = +2

Query: 2    GEKTGALETEEPPLPDGKKQNNRKGGAKK-TTTVVLDTDSENEVSDADDYDFEVVVQQPA 178
            GEK+ A+E+EEP +PD KKQ NR+GGAKK + T+VL++DS+NEV+D DD D   V ++ A
Sbjct: 1104 GEKSQAMESEEP-VPDMKKQINRRGGAKKKSNTIVLESDSDNEVNDVDDDDDFEVQEKAA 1162

Query: 179  P------------------EAGKMKVGRNLGTKRKLGQM----MLQPAEGTHITREE 283
            P                  +A      RN+GTK+  GQ     MLQPAE T  + E+
Sbjct: 1163 PVKKGGRKPAAQNAKKAPAKAPAAPKKRNVGTKQLSGQKLLTDMLQPAESTGTSPEK 1219


>dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subterraneum]
          Length = 1359

 Score = 74.7 bits (182), Expect = 2e-13
 Identities = 51/117 (43%), Positives = 69/117 (58%), Gaps = 23/117 (19%)
 Frame = +2

Query: 2    GEKTGALETEEPPLPDGKKQNNRKGGAKK-TTTVVLDTDSENEVSDADDYDFEVVVQQPA 178
            GEK+ A+E+EEP +PD KKQ NR+GGAKK + T+VL++DS+NEV+D DD D   V ++ A
Sbjct: 1148 GEKSQAMESEEP-VPDMKKQINRRGGAKKKSNTIVLESDSDNEVNDVDDDDDFEVQEKAA 1206

Query: 179  P------------------EAGKMKVGRNLGTKRKLGQM----MLQPAEGTHITREE 283
            P                  +A      RN+GTK+  GQ     MLQPAE T  + E+
Sbjct: 1207 PVKKGGRKPAAQNAKKAPAKAPAAPKKRNVGTKQLSGQKLLTDMLQPAESTGTSPEK 1263


>sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase
            II; AltName: Full=PsTopII
 emb|CAA74891.1| topoisomerase II [Pisum sativum]
          Length = 1462

 Score = 73.2 bits (178), Expect = 7e-13
 Identities = 52/124 (41%), Positives = 72/124 (58%), Gaps = 30/124 (24%)
 Frame = +2

Query: 2    GEKTGALETEE-PPLPDGKKQNNRKGGAKK-TTTVVLDTDSENEVSDA--DDYDFEVVVQ 169
            GEK+ A+E+EE      GKKQNN++GGAKK ++T+VL++DS+NEV+D   DD DFE V Q
Sbjct: 1244 GEKSQAMESEEVQQKAAGKKQNNKRGGAKKKSSTIVLESDSDNEVNDVDDDDDDFEEVQQ 1303

Query: 170  QPAP--EAGKMKVGRN--------------------LGTKRKLGQM----MLQPAEGTHI 271
            + AP  + G+    +N                    +GTK+  GQ     MLQPAEGT  
Sbjct: 1304 KAAPVKKGGRKPAAQNAKKAPAKAPAKAPAAPKKRSVGTKQSAGQKLLTDMLQPAEGTGT 1363

Query: 272  TREE 283
            + E+
Sbjct: 1364 SPEK 1367


>dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subterraneum]
          Length = 1336

 Score = 70.5 bits (171), Expect = 6e-12
 Identities = 50/112 (44%), Positives = 70/112 (62%), Gaps = 18/112 (16%)
 Frame = +2

Query: 2    GEKTGALETEEPPLPDGKKQNNRKGGAKK-TTTVVLDTDSENEVSDADDYDFEVVVQQPA 178
            GEK+GA+E+EE P+P GKKQ N++ GAKK  +T+VL++DS+NE    DD +FE V Q+ A
Sbjct: 1156 GEKSGAMESEE-PVPAGKKQINKRAGAKKMLSTIVLESDSDNE----DDGNFE-VQQKAA 1209

Query: 179  P-------------EAGKMKVGRNLGTKRKLGQM----MLQPAEGTHITREE 283
            P             +A  +   RN+GTK+ LG+     MLQPAE T  + E+
Sbjct: 1210 PTKKGGRKPAAQNTKAPALPKKRNVGTKQTLGKKLLTGMLQPAESTATSPEK 1261


>gb|PNY06816.1| DNA topoisomerase 2-like protein [Trifolium pratense]
          Length = 1254

 Score = 67.8 bits (164), Expect = 5e-11
 Identities = 47/105 (44%), Positives = 65/105 (61%), Gaps = 17/105 (16%)
 Frame = +2

Query: 2    GEKTGALETEEPPLPDGKKQNNRKGGAKK-TTTVVLDTDSENEVSDADDYDFEVVVQ-QP 175
            GEK+GA+E++E P+P GKKQ N +G AKK ++T+VL +DS+NE    DD +FEV  +  P
Sbjct: 1074 GEKSGAMESDE-PVPAGKKQINERGDAKKMSSTIVLGSDSDNE----DDDNFEVQQKAAP 1128

Query: 176  APEAGKMKVG-----------RNLGTKRKLGQM----MLQPAEGT 265
            A + G+               RN+GTK+ LG+     MLQPAE T
Sbjct: 1129 AKKGGRKPAAQNAKAPALPKKRNVGTKQTLGKKLLTGMLQPAEST 1173


>ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago truncatula]
 gb|AES69522.2| DNA topoisomerase IV subunit A [Medicago truncatula]
          Length = 1504

 Score = 66.2 bits (160), Expect = 2e-10
 Identities = 35/74 (47%), Positives = 51/74 (68%), Gaps = 4/74 (5%)
 Frame = +2

Query: 2    GEKTGALETEEPPLPDGKKQNNRKGGAKK-TTTVVLDTDSENEVSDADDYDFEVVVQQ-- 172
            GEK+ A+E E P  P GKKQ N++GGAKK + T+VL++DS+NEV+D +D D +  VQ+  
Sbjct: 1263 GEKSQAMEEEPPVPPAGKKQINKRGGAKKKSNTIVLESDSDNEVNDVEDDDDDFEVQENA 1322

Query: 173  -PAPEAGKMKVGRN 211
             PA + G+    +N
Sbjct: 1323 APAKKGGRKPAAQN 1336


>dbj|GAU30192.1| hypothetical protein TSUD_311430 [Trifolium subterraneum]
          Length = 1252

 Score = 63.9 bits (154), Expect = 1e-09
 Identities = 45/107 (42%), Positives = 65/107 (60%), Gaps = 13/107 (12%)
 Frame = +2

Query: 2    GEKTGALETEEPPLPDGKKQNNRKGGAKK-TTTVVLDTDSENEVSDADDYDFEVVVQ-QP 175
            GEK+ A+E+EEP +P GKK  N++ GAKK ++T+VL+ DS+NE    DD +FEV  +  P
Sbjct: 1076 GEKSEAMESEEP-VPAGKKPINKRAGAKKMSSTIVLEPDSDNE----DDDNFEVQQKATP 1130

Query: 176  APEAGKMKVGRN-----------LGTKRKLGQMMLQPAEGTHITREE 283
            A + G+    +N           +GTK+ L   MLQPAE T  + E+
Sbjct: 1131 AKKGGRKPAAQNNKAPALPKKRKVGTKQILLTDMLQPAESTATSPEK 1177


>ref|XP_003601827.1| DNA topoisomerase IV subunit A [Medicago truncatula]
 gb|AES72078.1| DNA topoisomerase IV subunit A [Medicago truncatula]
          Length = 1345

 Score = 63.5 bits (153), Expect = 2e-09
 Identities = 40/96 (41%), Positives = 57/96 (59%), Gaps = 1/96 (1%)
 Frame = +2

Query: 2    GEKTGALETEEPPLPDGKKQNNRKGGA-KKTTTVVLDTDSENEVSDADDYDFEVVVQQPA 178
            GEK+GA+E+EE P+P GKKQ N++GGA KK++T+ L++DS+NEV D DD DFE       
Sbjct: 1190 GEKSGAMESEE-PVPAGKKQINKRGGAKKKSSTIGLESDSDNEVIDDDD-DFESAESTAT 1247

Query: 179  PEAGKMKVGRNLGTKRKLGQMMLQPAEGTHITREED 286
                K++  R     +K    +L  A      + ED
Sbjct: 1248 SPEKKVRKMRESPFNKKSSGSILDRAANKDTNQIED 1283


>gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium pratense]
          Length = 1492

 Score = 62.0 bits (149), Expect = 6e-09
 Identities = 46/107 (42%), Positives = 63/107 (58%), Gaps = 21/107 (19%)
 Frame = +2

Query: 2    GEKTGALETEEPPLPDGKKQNNRKGGAKK-TTTVVLDTDSE-NEVSDADDYDFEVVV--- 166
            GEK+ A+E+EEP +P  KKQ NR GGAKK ++T+VLD+D+E N+V D DD++        
Sbjct: 1287 GEKSQAMESEEP-VPAMKKQINRGGGAKKKSSTIVLDSDNEVNDVDDDDDFEVHEKAAPV 1345

Query: 167  ----QQPAPEAGKMKVG--------RNLGTKRKLGQM----MLQPAE 259
                ++PA +  K            RN+GTK+  GQ     MLQPAE
Sbjct: 1346 KKGGRKPAAQNAKKAPAKAPAAPKKRNVGTKQLSGQKILTDMLQPAE 1392


>ref|XP_014501355.1| DNA topoisomerase 2 [Vigna radiata var. radiata]
          Length = 1492

 Score = 58.5 bits (140), Expect = 9e-08
 Identities = 33/70 (47%), Positives = 46/70 (65%), Gaps = 2/70 (2%)
 Frame = +2

Query: 5    EKTGALETEEPPLPDGKKQNNRKG-GAKKTTTVVLD-TDSENEVSDADDYDFEVVVQQPA 178
            +++  +ETEE P P GK    R+G  AKK+T +VLD TDS+N+  D +D DFE+   QP+
Sbjct: 1267 DQSTGMETEERPAPAGKTAPKRRGPAAKKSTAIVLDETDSDNDNDDTNDNDFEIT--QPS 1324

Query: 179  PEAGKMKVGR 208
              +GK K GR
Sbjct: 1325 AGSGKKKGGR 1334


>gb|KRH32967.1| hypothetical protein GLYMA_10G090000 [Glycine max]
          Length = 1459

 Score = 56.6 bits (135), Expect = 5e-07
 Identities = 32/70 (45%), Positives = 45/70 (64%), Gaps = 4/70 (5%)
 Frame = +2

Query: 11   TGALETEEPPLPDGKKQ-NNRKGGAKKTTTVVL--DTDSENEVSDADDYDFEVVVQQP-A 178
            +  +ETEEPP P  KK+   R+G AKK ++ ++  D+DS+NE+ D +D D    + QP A
Sbjct: 1247 SAGMETEEPPAPAEKKEAPKRRGAAKKMSSAIVLDDSDSDNEIHDVEDDDDNFEIMQPVA 1306

Query: 179  PEAGKMKVGR 208
             EAGK K GR
Sbjct: 1307 LEAGKKKGGR 1316


>ref|XP_014618555.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisomerase 2-like [Glycine
            max]
          Length = 1481

 Score = 56.6 bits (135), Expect = 5e-07
 Identities = 32/70 (45%), Positives = 45/70 (64%), Gaps = 4/70 (5%)
 Frame = +2

Query: 11   TGALETEEPPLPDGKKQ-NNRKGGAKKTTTVVL--DTDSENEVSDADDYDFEVVVQQP-A 178
            +  +ETEEPP P  KK+   R+G AKK ++ ++  D+DS+NE+ D +D D    + QP A
Sbjct: 1269 SAGMETEEPPAPAEKKEAPKRRGAAKKMSSAIVLDDSDSDNEIHDVEDDDDNFEIMQPVA 1328

Query: 179  PEAGKMKVGR 208
             EAGK K GR
Sbjct: 1329 LEAGKKKGGR 1338


>gb|KYP71391.1| DNA topoisomerase 2 [Cajanus cajan]
          Length = 1446

 Score = 54.7 bits (130), Expect = 2e-06
 Identities = 30/77 (38%), Positives = 46/77 (59%), Gaps = 4/77 (5%)
 Frame = +2

Query: 11   TGALETEEPPLPDGKKQNNRKGGA--KKTTTVVLDTDSENEV--SDADDYDFEVVVQQPA 178
            +  +ETEEPP P GKK+  ++ G   KK++ +VLD    ++   +D++D DFE++  QP 
Sbjct: 1234 SAGMETEEPPAPAGKKEAPKRRGPAKKKSSAIVLDDSDSDDAAKNDSEDDDFEII--QPV 1291

Query: 179  PEAGKMKVGRNLGTKRK 229
              AGK K GR     +K
Sbjct: 1292 AAAGKKKGGRKPAAAKK 1308


>ref|XP_020212372.1| DNA topoisomerase 2 [Cajanus cajan]
          Length = 1480

 Score = 54.7 bits (130), Expect = 2e-06
 Identities = 30/77 (38%), Positives = 46/77 (59%), Gaps = 4/77 (5%)
 Frame = +2

Query: 11   TGALETEEPPLPDGKKQNNRKGGA--KKTTTVVLDTDSENEV--SDADDYDFEVVVQQPA 178
            +  +ETEEPP P GKK+  ++ G   KK++ +VLD    ++   +D++D DFE++  QP 
Sbjct: 1268 SAGMETEEPPAPAGKKEAPKRRGPAKKKSSAIVLDDSDSDDAAKNDSEDDDFEII--QPV 1325

Query: 179  PEAGKMKVGRNLGTKRK 229
              AGK K GR     +K
Sbjct: 1326 AAAGKKKGGRKPAAAKK 1342


>ref|XP_015936924.1| DNA topoisomerase 2 isoform X2 [Arachis duranensis]
          Length = 1490

 Score = 53.5 bits (127), Expect = 5e-06
 Identities = 38/106 (35%), Positives = 54/106 (50%), Gaps = 21/106 (19%)
 Frame = +2

Query: 5    EKTGALETEEPPLPDGKKQNNRKGGAKK--TTTVVLDTDSENEVSDADDYDFEV-VVQQP 175
            + + A+E E PP     ++    G AKK  ++T++LD+   + V D DD D +  ++ Q 
Sbjct: 1274 QSSAAMEAEVPPEAPAARKGKTTGRAKKKSSSTILLDSSDSDNVEDEDDEDDDFEILDQA 1333

Query: 176  APEAGKMKVG----------------RNLGTKR--KLGQMMLQPAE 259
            A EAGK K G                RNLG+KR  KL   ML+PAE
Sbjct: 1334 ASEAGKKKGGRKPAAQKAAKAPAATKRNLGSKRSQKLSTEMLKPAE 1379


>ref|XP_015936922.1| DNA topoisomerase 2 isoform X1 [Arachis duranensis]
          Length = 1521

 Score = 53.5 bits (127), Expect = 5e-06
 Identities = 38/106 (35%), Positives = 54/106 (50%), Gaps = 21/106 (19%)
 Frame = +2

Query: 5    EKTGALETEEPPLPDGKKQNNRKGGAKK--TTTVVLDTDSENEVSDADDYDFEV-VVQQP 175
            + + A+E E PP     ++    G AKK  ++T++LD+   + V D DD D +  ++ Q 
Sbjct: 1305 QSSAAMEAEVPPEAPAARKGKTTGRAKKKSSSTILLDSSDSDNVEDEDDEDDDFEILDQA 1364

Query: 176  APEAGKMKVG----------------RNLGTKR--KLGQMMLQPAE 259
            A EAGK K G                RNLG+KR  KL   ML+PAE
Sbjct: 1365 ASEAGKKKGGRKPAAQKAAKAPAATKRNLGSKRSQKLSTEMLKPAE 1410


>ref|XP_017425524.1| PREDICTED: DNA topoisomerase 2 [Vigna angularis]
 gb|KOM42453.1| hypothetical protein LR48_Vigan05g005700 [Vigna angularis]
 dbj|BAT93356.1| hypothetical protein VIGAN_07230300 [Vigna angularis var. angularis]
          Length = 1480

 Score = 53.1 bits (126), Expect = 7e-06
 Identities = 32/65 (49%), Positives = 42/65 (64%), Gaps = 2/65 (3%)
 Frame = +2

Query: 20   LETEEPPLPDGKKQNNRKG-GAKKTTTVVL-DTDSENEVSDADDYDFEVVVQQPAPEAGK 193
            +ETEE P P GK    R+G  AKK++TVVL DTDS+N+  D++D DF  + Q     +GK
Sbjct: 1271 METEERPAPTGKTAPKRRGPAAKKSSTVVLDDTDSDNDNDDSNDNDFG-ITQPSTAGSGK 1329

Query: 194  MKVGR 208
             K GR
Sbjct: 1330 KKGGR 1334


>ref|XP_016170361.1| DNA topoisomerase 2 isoform X2 [Arachis ipaensis]
          Length = 1486

 Score = 53.1 bits (126), Expect = 7e-06
 Identities = 37/105 (35%), Positives = 53/105 (50%), Gaps = 20/105 (19%)
 Frame = +2

Query: 5    EKTGALETEEPP-LPDGKKQNNRKGGAKKTTTVVLDTDSENEVSDADDYDFEV-VVQQPA 178
            + + A+E E PP  P  +K    +   K ++T++LD+   + V D DD D +  ++ Q A
Sbjct: 1274 QSSAAMEAEAPPEAPAARKGKTGRAKKKSSSTILLDSSDSDNVEDEDDGDDDFEILDQAA 1333

Query: 179  PEAGKMKVG----------------RNLGTKR--KLGQMMLQPAE 259
             EAGK K G                RNLG+KR  KL   ML+PAE
Sbjct: 1334 SEAGKKKGGRKPAAHKAAKAPAATKRNLGSKRSQKLSTEMLKPAE 1378


>ref|XP_016170360.1| DNA topoisomerase 2 isoform X1 [Arachis ipaensis]
          Length = 1517

 Score = 53.1 bits (126), Expect = 7e-06
 Identities = 37/105 (35%), Positives = 53/105 (50%), Gaps = 20/105 (19%)
 Frame = +2

Query: 5    EKTGALETEEPP-LPDGKKQNNRKGGAKKTTTVVLDTDSENEVSDADDYDFEV-VVQQPA 178
            + + A+E E PP  P  +K    +   K ++T++LD+   + V D DD D +  ++ Q A
Sbjct: 1305 QSSAAMEAEAPPEAPAARKGKTGRAKKKSSSTILLDSSDSDNVEDEDDGDDDFEILDQAA 1364

Query: 179  PEAGKMKVG----------------RNLGTKR--KLGQMMLQPAE 259
             EAGK K G                RNLG+KR  KL   ML+PAE
Sbjct: 1365 SEAGKKKGGRKPAAHKAAKAPAATKRNLGSKRSQKLSTEMLKPAE 1409