BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus24_contig00014217
         (1385 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003605649.2| cytochrome P450 family 81 protein [Medicago ...   524   0.0  
ref|XP_013457401.1| cytochrome P450 family 81 protein [Medicago ...   524   0.0  
ref|XP_014506448.1| isoflavone 3'-hydroxylase [Vigna radiata var...   496   e-170
ref|XP_007158858.1| hypothetical protein PHAVU_002G187800g [Phas...   491   e-168
ref|XP_003532671.1| PREDICTED: isoflavone 3'-hydroxylase-like [G...   490   e-167
ref|XP_017405622.1| PREDICTED: isoflavone 3'-hydroxylase-like [V...   488   e-166
ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [C...   448   e-151
sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase;...   447   e-150
ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago ...   447   e-150
dbj|BAT21113.1| cytochrome P450 [synthetic construct]                 437   e-147
ref|XP_003611658.1| cytochrome P450 family 81 protein [Medicago ...   438   e-147
sp|Q6WNQ8.1|C81E8_MEDTR RecName: Full=Cytochrome P450 81E8 >gi|3...   438   e-147
sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase;...   437   e-147
ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [C...   435   e-146
ref|XP_003548043.3| PREDICTED: cytochrome P450 81E8 [Glycine max...   434   e-145
gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceu...   433   e-145
dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]                432   e-144
ref|XP_016197998.1| isoflavone 3'-hydroxylase [Arachis ipaensis]      430   e-143
ref|XP_016193629.1| isoflavone 3'-hydroxylase [Arachis ipaensis]      429   e-143
ref|XP_016201681.2| cytochrome P450 81E8 [Arachis ipaensis]           428   e-143

>ref|XP_003605649.2| cytochrome P450 family 81 protein [Medicago truncatula]
 gb|AES87846.2| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 491

 Score =  524 bits (1350), Expect = 0.0
 Identities = 261/376 (69%), Positives = 309/376 (82%), Gaps = 2/376 (0%)
 Frame = +2

Query: 263  LITFKLLLERKRSFKNLPPSPP-TLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSR 439
            + T KLLL+R R FKNLPPSP  TLP+IG+LH I LPLHRSL+NLS KYGNIF LW GSR
Sbjct: 15   IFTLKLLLQR-RKFKNLPPSPSITLPLIGHLHYINLPLHRSLHNLSQKYGNIFSLWFGSR 73

Query: 440  RVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLD 619
            RVVVISS  L+++C  K+D+VLSNRPHFLTGKYLFY+YTSLDSAPYGDH+RNLR+IITLD
Sbjct: 74   RVVVISSQALLQQCLAKHDVVLSNRPHFLTGKYLFYNYTSLDSAPYGDHWRNLRKIITLD 133

Query: 620  VLSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAGDV-E 796
            +LST RLNSF + RR GT++L+T L  D+C+GFTRVELR RLTDM F+ M  MV GD  E
Sbjct: 134  ILSTQRLNSFRETRRKGTLKLITNLVRDSCDGFTRVELRTRLTDMMFDNMTAMVVGDAAE 193

Query: 797  GKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADAFLQELVEELRDG 976
             K+FKE++SEM+PLF A+NK               VKK+K +GKR D FLQ +VEE++ G
Sbjct: 194  FKKFKEMVSEMMPLFGANNKGDFVPLLRLFDFDGMVKKMKDIGKRGDLFLQGIVEEIKSG 253

Query: 977  KSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEWAISALLNHGEIL 1156
            K + N M+ HLLTLQKSQPEYYSD+IIKGLIQG FLGGTDT   T+EWA+SALLN+ EIL
Sbjct: 254  KHNENTMIQHLLTLQKSQPEYYSDEIIKGLIQGTFLGGTDTIVTTLEWAMSALLNNPEIL 313

Query: 1157 KKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPRYSSEEFNIGGFT 1336
            KKAKDEL T +G+DR+VDESDI NLPY+QNII+ET+RLYSPAPLLLPR+SS+E NI G+T
Sbjct: 314  KKAKDELYTKIGNDRLVDESDISNLPYIQNIIYETLRLYSPAPLLLPRFSSDECNIEGYT 373

Query: 1337 VPRETIVLINAWAIHR 1384
            +PR+TIVLINAWAI R
Sbjct: 374  IPRDTIVLINAWAIQR 389


>ref|XP_013457401.1| cytochrome P450 family 81 protein [Medicago truncatula]
 gb|KEH31432.1| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 494

 Score =  524 bits (1349), Expect = 0.0
 Identities = 264/376 (70%), Positives = 308/376 (81%), Gaps = 2/376 (0%)
 Frame = +2

Query: 263  LITFKLLLERKRSFKNLPPSPP-TLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSR 439
            ++T KLLL+R R FKNLPP P  +LPIIGNLHNI LPLHRSL+NLS KYG IF LW GS 
Sbjct: 18   IVTLKLLLQR-RKFKNLPPGPSISLPIIGNLHNINLPLHRSLHNLSQKYGKIFTLWFGSS 76

Query: 440  RVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLD 619
            RVVVISS TL+++C TK+D+ LSNRP FLTGKYLFY+YTSLDSAPYGDH+RNLRRIITLD
Sbjct: 77   RVVVISSQTLLQQCLTKHDVDLSNRPRFLTGKYLFYNYTSLDSAPYGDHWRNLRRIITLD 136

Query: 620  VLSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAGDVE- 796
            +LST RLNSF   RR+ T++L+ KLA DT E FTRVELR RLTDMTF  M +MVAGD E 
Sbjct: 137  ILSTQRLNSFVGSRRSETLKLIIKLAKDTSERFTRVELRTRLTDMTFEIMTKMVAGDAEE 196

Query: 797  GKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADAFLQELVEELRDG 976
            GK+FKE+I+EM+PLF ASN                VK++K +GKR D+FLQ +VEE+R G
Sbjct: 197  GKKFKEMINEMMPLFGASNMGDFVPLLRLFDFDGLVKRMKDIGKRGDSFLQGIVEEIRSG 256

Query: 977  KSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEWAISALLNHGEIL 1156
            K   N ML HLLTLQKSQP+YYSD+IIKGL+QGMFL GTDT+AVT+EWAI+ LLNH  IL
Sbjct: 257  KHGDNNMLQHLLTLQKSQPDYYSDEIIKGLVQGMFLAGTDTTAVTLEWAIATLLNHPNIL 316

Query: 1157 KKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPRYSSEEFNIGGFT 1336
            KKAKDEL T +G+DR+V+ESDIPNL Y+QNII+ET+RLYSPAPLLLPR+SS E NI GFT
Sbjct: 317  KKAKDELNTQIGYDRLVEESDIPNLSYIQNIIYETLRLYSPAPLLLPRFSSNECNIEGFT 376

Query: 1337 VPRETIVLINAWAIHR 1384
            +PR+TIVLINAWAI R
Sbjct: 377  IPRDTIVLINAWAIQR 392


>ref|XP_014506448.1| isoflavone 3'-hydroxylase [Vigna radiata var. radiata]
          Length = 500

 Score =  496 bits (1276), Expect = e-170
 Identities = 250/386 (64%), Positives = 302/386 (78%), Gaps = 12/386 (3%)
 Frame = +2

Query: 263  LITFKLLLERKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSRR 442
            + TFK LL R+R  +NLPP PPTLPIIGNLH I  PLHRSL +LS +YG IF LW GSR 
Sbjct: 15   IFTFKFLLRRRR-LENLPPHPPTLPIIGNLHLIKPPLHRSLLSLSQQYGPIFSLWFGSRF 73

Query: 443  VVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLDV 622
            VVVI+SPTLV+ECFTKYDIVL+NRP FLTGKYLFY+YTS+ S+ YGDH+RNLRRIIT+DV
Sbjct: 74   VVVITSPTLVQECFTKYDIVLANRPRFLTGKYLFYNYTSMGSSSYGDHWRNLRRIITIDV 133

Query: 623  LSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAG----- 787
            LST RLNSF ++RR  TM ++ KL  +TC+GF  V++R RLT+MTFN MMRM++G     
Sbjct: 134  LSTQRLNSFFEVRREETMRVMQKLVRETCKGFALVQIRPRLTEMTFNNMMRMISGKRYYG 193

Query: 788  ---DV----EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADAFL 946
               DV    E KQF+ II+E++ L  A+NK                K+LK +  RADAFL
Sbjct: 194  DDGDVTDAEEAKQFRAIITEVMSLLGANNKGDFLPLLRWFDFDGLEKRLKSISTRADAFL 253

Query: 947  QELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEWAI 1126
            Q L+EE R GK   N M+ HLLT+Q++QPEYYSD IIKGLIQGM L GTDT+AVT+EWA+
Sbjct: 254  QGLLEEHRSGKHKTNTMIEHLLTMQETQPEYYSDHIIKGLIQGMLLAGTDTTAVTMEWAM 313

Query: 1127 SALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPRYS 1306
            ++LLNH EILKKA+DE++  VG DR+VDESDIPNLPYLQNII+ET+RL+SPAP+LLP YS
Sbjct: 314  ASLLNHPEILKKAQDEIDNCVGEDRLVDESDIPNLPYLQNIIYETLRLFSPAPMLLPHYS 373

Query: 1307 SEEFNIGGFTVPRETIVLINAWAIHR 1384
            SE+F +GGFTVPR+TIVLINAWAI R
Sbjct: 374  SEQFTLGGFTVPRDTIVLINAWAIQR 399


>ref|XP_007158858.1| hypothetical protein PHAVU_002G187800g [Phaseolus vulgaris]
 gb|ESW30852.1| hypothetical protein PHAVU_002G187800g [Phaseolus vulgaris]
          Length = 500

 Score =  491 bits (1263), Expect = e-168
 Identities = 248/386 (64%), Positives = 298/386 (77%), Gaps = 12/386 (3%)
 Frame = +2

Query: 263  LITFKLLLERKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSRR 442
            + T K LL+R+R  KNLPP PPTLPIIGNLH I  PLHRS  +LS KYG IF LW GSR 
Sbjct: 15   IFTLKFLLQRRR-LKNLPPHPPTLPIIGNLHLIKPPLHRSFLSLSQKYGPIFSLWFGSRY 73

Query: 443  VVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLDV 622
            VVVI+SPTLV+ECFTKYD VL+NRP  LTGKYLFY+YTS+ S+PYG+H+RNLRRIIT+DV
Sbjct: 74   VVVITSPTLVQECFTKYDTVLANRPRLLTGKYLFYNYTSMGSSPYGEHWRNLRRIITIDV 133

Query: 623  LSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVA------ 784
            LST RLNSF +IRR  TM ++ KL  +TC+GFT V++R RLT+MTFN MMRM++      
Sbjct: 134  LSTQRLNSFFEIRREETMRVIQKLVRETCKGFTHVQIRPRLTEMTFNNMMRMISGKRYYG 193

Query: 785  --GDV----EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADAFL 946
              GDV    E KQF++IISE++ L  A+NK                K+LK + +RADAFL
Sbjct: 194  DDGDVTDAEEAKQFRDIISEVMSLLGANNKGDFLPLLRWFDFDGLEKRLKSISRRADAFL 253

Query: 947  QELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEWAI 1126
            Q L+EE R GK   N M+ HLLT+Q+SQPEYYSD IIKGLIQGM L GTDT+AVT+EWA+
Sbjct: 254  QGLLEEHRSGKHKANTMIEHLLTMQESQPEYYSDHIIKGLIQGMLLAGTDTTAVTMEWAV 313

Query: 1127 SALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPRYS 1306
            ++LLN  EILKKA+DE++  VG DR+V+ESDIPNLPYLQNII+ET+RL+SPAPLLLP YS
Sbjct: 314  ASLLNEPEILKKAQDEIKKCVGEDRLVEESDIPNLPYLQNIIYETLRLFSPAPLLLPHYS 373

Query: 1307 SEEFNIGGFTVPRETIVLINAWAIHR 1384
            S+   IGGFT+P  TIVLINAWAI R
Sbjct: 374  SDHCTIGGFTIPPHTIVLINAWAIQR 399


>ref|XP_003532671.1| PREDICTED: isoflavone 3'-hydroxylase-like [Glycine max]
 gb|KHN28918.1| Isoflavone 2'-hydroxylase [Glycine soja]
 gb|KRH42430.1| hypothetical protein GLYMA_08G089400 [Glycine max]
          Length = 500

 Score =  490 bits (1261), Expect = e-167
 Identities = 245/389 (62%), Positives = 299/389 (76%), Gaps = 12/389 (3%)
 Frame = +2

Query: 254  AFILITFKLLLERKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLG 433
            + +++   L   ++R  KNLPP PP+LP+IGNLH I  PLHRSL +LS KYG IF LW G
Sbjct: 8    SLLVLVLILRFVQRRRVKNLPPCPPSLPLIGNLHYIKSPLHRSLLSLSEKYGPIFSLWFG 67

Query: 434  SRRVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIIT 613
            SR VVVISSPTL++ECFTK+DIVL+NRP FLTGKYLFY+Y+S+ S+PYGDH+RNLRRIIT
Sbjct: 68   SRFVVVISSPTLLQECFTKHDIVLANRPRFLTGKYLFYNYSSMGSSPYGDHWRNLRRIIT 127

Query: 614  LDVLSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAG-- 787
            +DVLST RLNSF +IRR  TM ++ KLA +TC GF  V LR RLT+MTFN MMRM++G  
Sbjct: 128  IDVLSTSRLNSFFEIRREETMRVIQKLARETCNGFALVHLRPRLTEMTFNNMMRMISGKR 187

Query: 788  ----DV------EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRAD 937
                D+      E KQF++I++E++ L  A+NK                K+LK +  RAD
Sbjct: 188  YYGDDIEAADAEEAKQFRDIMTEVMSLLGANNKGDFLPFLRWFDFDGLEKRLKVISTRAD 247

Query: 938  AFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIE 1117
            +FLQ L+EE R GK   N M+ HLLT+Q+SQP YYSD IIKGLIQGM L GTDT+AV IE
Sbjct: 248  SFLQGLLEEHRSGKHKANTMIEHLLTMQESQPHYYSDHIIKGLIQGMLLAGTDTTAVAIE 307

Query: 1118 WAISALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLP 1297
            WA+S+LLNH EILKKAKDE++ +VG DR+VDESDIP LPYLQNII+ET+RL++PAPLLLP
Sbjct: 308  WAVSSLLNHPEILKKAKDEIDNMVGQDRLVDESDIPKLPYLQNIIYETLRLFAPAPLLLP 367

Query: 1298 RYSSEEFNIGGFTVPRETIVLINAWAIHR 1384
             YSSEE  IGGFT+PR+TIVLINAWAI R
Sbjct: 368  HYSSEECTIGGFTIPRDTIVLINAWAIQR 396


>ref|XP_017405622.1| PREDICTED: isoflavone 3'-hydroxylase-like [Vigna angularis]
 gb|KOM25512.1| hypothetical protein LR48_Vigan107s003100 [Vigna angularis]
 dbj|BAT74197.1| hypothetical protein VIGAN_01181600 [Vigna angularis var. angularis]
          Length = 500

 Score =  488 bits (1255), Expect = e-166
 Identities = 247/386 (63%), Positives = 299/386 (77%), Gaps = 12/386 (3%)
 Frame = +2

Query: 263  LITFKLLLERKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSRR 442
            + T K LL R+R  +NLPP PPTLPIIGNLH I  PLHRSL +LS +YG IF LW GSR 
Sbjct: 15   IFTLKFLLRRRR-LENLPPHPPTLPIIGNLHLIKPPLHRSLLSLSQQYGPIFSLWFGSRF 73

Query: 443  VVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLDV 622
            VVVI+SPTLV+ECFTKYD VL+NRP FLTGKYLFY+YTS+ S+PYGDH+RNLRRIIT+DV
Sbjct: 74   VVVITSPTLVQECFTKYDTVLANRPRFLTGKYLFYNYTSMGSSPYGDHWRNLRRIITIDV 133

Query: 623  LSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVA------ 784
            LST RLNSF ++RR  TM +V KL  +T +GF  V++R RLT+MTFN MMRM++      
Sbjct: 134  LSTQRLNSFFEVRREETMRVVQKLVRETSKGFALVQIRPRLTEMTFNNMMRMISGKRYYG 193

Query: 785  --GDV----EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADAFL 946
              GDV    E KQF+ II+E++ L  A+NK                K+LK +  RADAFL
Sbjct: 194  DDGDVTDAEEAKQFRAIITEVMSLLGANNKGDFLPLLRWFDFDGLEKRLKSISTRADAFL 253

Query: 947  QELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEWAI 1126
            Q L+EE R GK   N M+ HLLT+Q++QPEYYSD IIKGLIQGM L GTDT+AVT+EWA+
Sbjct: 254  QGLLEEHRSGKHKTNTMIEHLLTMQETQPEYYSDHIIKGLIQGMLLAGTDTTAVTMEWAM 313

Query: 1127 SALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPRYS 1306
            ++LLNH EILKKA+DE++  VG DR+VDESDIPNLPY+QNII+ET+RL+SPAP+LLP YS
Sbjct: 314  ASLLNHPEILKKAQDEIDKCVGQDRLVDESDIPNLPYIQNIIYETLRLFSPAPMLLPHYS 373

Query: 1307 SEEFNIGGFTVPRETIVLINAWAIHR 1384
            SE+  +GGFTVPR+TIVLINAWAI R
Sbjct: 374  SEQCTLGGFTVPRDTIVLINAWAIQR 399


>ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
          Length = 500

 Score =  448 bits (1152), Expect = e-151
 Identities = 225/389 (57%), Positives = 289/389 (74%), Gaps = 15/389 (3%)
 Frame = +2

Query: 263  LITFKLLLE-RKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSR 439
            +IT K++L+ + R  KNLPP PPT+PIIGNLH++  P+HR+   LS KYG++  LW GSR
Sbjct: 15   IITIKIILQIQSRKLKNLPPGPPTIPIIGNLHHLKHPIHRTFTTLSQKYGDVLSLWFGSR 74

Query: 440  RVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLD 619
             VVV+SSP+LV ECFTK DI+L+NRP FLTGKY+F +YT+L SA YGDH+RNLRRI T+D
Sbjct: 75   LVVVVSSPSLVNECFTKNDIILANRPQFLTGKYIFNNYTTLGSASYGDHWRNLRRITTID 134

Query: 620  VLSTHRLNSFSDIRRAGTMELVTKLASDTC-EGFTRVELRKRLTDMTFNTMMRMVAG--- 787
            VLS +RLNSF  +R   T  LV KL  +T  EGFT+VELR RLT+MTFN MMRM++G   
Sbjct: 135  VLSNNRLNSFLGVRTDETNRLVQKLLKNTISEGFTKVELRHRLTEMTFNGMMRMISGKRY 194

Query: 788  --------DVE-GKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADA 940
                    DVE  KQF++IISE++ L  A+NK                K+ K + KRADA
Sbjct: 195  YGDDGDVTDVEEAKQFRDIISEIMSLLGANNKGDFLPLLRLFDLDDLEKRCKRIAKRADA 254

Query: 941  FLQELVEELRDG-KSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIE 1117
            FLQ L+EE++ G ++ G+ M+ HLL L++SQPEYYSD +IKGLIQ M L GTDTSAV IE
Sbjct: 255  FLQGLIEEIQSGNQNDGDTMIDHLLKLRESQPEYYSDHMIKGLIQAMLLAGTDTSAVAIE 314

Query: 1118 WAISALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLP 1297
            W +S LLNH ++L KAK+E+ET +G D++++E D+  LPYLQNII+ET+RL+ PAPLLLP
Sbjct: 315  WVMSELLNHPDVLNKAKEEIETQIGRDKLIEEHDLSKLPYLQNIINETLRLHPPAPLLLP 374

Query: 1298 RYSSEEFNIGGFTVPRETIVLINAWAIHR 1384
             YSSE+F +GGF VP++TI+L N WAIHR
Sbjct: 375  HYSSEDFTLGGFNVPKDTIILSNVWAIHR 403


>sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase; AltName: Full=Cytochrome
            P450 81E9
 gb|AAQ20041.1| isoflavone 3'-hydroxylase, partial [Medicago truncatula]
          Length = 501

 Score =  447 bits (1149), Expect = e-150
 Identities = 231/392 (58%), Positives = 282/392 (71%), Gaps = 18/392 (4%)
 Frame = +2

Query: 263  LITFKLLLE-RKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSR 439
            +IT K+LL+   R  KNLPP PPT+PIIGNLH++  PLHR+   LS  YG+IF LW GSR
Sbjct: 13   IITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSR 72

Query: 440  RVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLD 619
             VVV+SSP+L  ECFTK DI+L+NRP FLTGKY+FY+YT+L SA YGDH+RNLRRI T+D
Sbjct: 73   LVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTID 132

Query: 620  VLSTHRLNSFSDIRRAGTMELVTKLASDTCE---GFTRVELRKRLTDMTFNTMMRMVA-- 784
            VLS +RLNSF  +RR  T  L+ KL  D      GFT+VELR RLT+MTFN MMRM++  
Sbjct: 133  VLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMISGK 192

Query: 785  ------GDV----EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRA 934
                  GDV    E KQF+EIISEM+ L  A+NK                K+ K + KR+
Sbjct: 193  RYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIAKRS 252

Query: 935  DAFLQELVEELRDGK--SSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAV 1108
            +AFL+ L+EE R G   S G  M+ HLL L +SQPEYYSD +IKGLIQGM L GTDTSAV
Sbjct: 253  NAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAV 312

Query: 1109 TIEWAISALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPL 1288
            TIEW +S LLNH E+LKKAK+EL+T +G +++VDE D+  LPYLQNII ET+RL+ PAPL
Sbjct: 313  TIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPL 372

Query: 1289 LLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 1384
            LLP YSSE+  IG F VP++TI+L N W IHR
Sbjct: 373  LLPHYSSEDCTIGEFNVPKDTIILTNVWGIHR 404


>ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago truncatula]
 gb|KEH37344.1| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 503

 Score =  447 bits (1149), Expect = e-150
 Identities = 231/392 (58%), Positives = 282/392 (71%), Gaps = 18/392 (4%)
 Frame = +2

Query: 263  LITFKLLLE-RKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSR 439
            +IT K+LL+   R  KNLPP PPT+PIIGNLH++  PLHR+   LS  YG+IF LW GSR
Sbjct: 15   IITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSR 74

Query: 440  RVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLD 619
             VVV+SSP+L  ECFTK DI+L+NRP FLTGKY+FY+YT+L SA YGDH+RNLRRI T+D
Sbjct: 75   LVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTID 134

Query: 620  VLSTHRLNSFSDIRRAGTMELVTKLASDTCE---GFTRVELRKRLTDMTFNTMMRMVA-- 784
            VLS +RLNSF  +RR  T  L+ KL  D      GFT+VELR RLT+MTFN MMRM++  
Sbjct: 135  VLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMISGK 194

Query: 785  ------GDV----EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRA 934
                  GDV    E KQF+EIISEM+ L  A+NK                K+ K + KR+
Sbjct: 195  RYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRLFDLDNLEKRCKRIAKRS 254

Query: 935  DAFLQELVEELRDGK--SSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAV 1108
            +AFL+ L+EE R G   S G  M+ HLL L +SQPEYYSD +IKGLIQGM L GTDTSAV
Sbjct: 255  NAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAV 314

Query: 1109 TIEWAISALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPL 1288
            TIEW +S LLNH E+LKKAK+EL+T +G +++VDE D+  LPYLQNII ET+RL+ PAPL
Sbjct: 315  TIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPL 374

Query: 1289 LLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 1384
            LLP YSSE+  IG F VP++TI+L N W IHR
Sbjct: 375  LLPHYSSEDCTIGEFNVPKDTIILTNVWGIHR 406


>dbj|BAT21113.1| cytochrome P450 [synthetic construct]
          Length = 477

 Score =  437 bits (1124), Expect = e-147
 Identities = 215/377 (57%), Positives = 273/377 (72%), Gaps = 12/377 (3%)
 Frame = +2

Query: 290  RKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSRRVVVISSPTL 469
            R R FKNLPP PP+LPIIGNLH++  PLHR+   LS KYG++F LW GSR VVV+SS + 
Sbjct: 3    RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASE 62

Query: 470  VKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLDVLSTHRLNSF 649
             ++CFTK D+VL+NRP FL+GKY+FY+YT+L S  YG+H+RNLRRI  LDVLS HR+NSF
Sbjct: 63   FQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSF 122

Query: 650  SDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAGD------------V 793
            S IRR  T  L+T+LA D+   F  +EL  RL DMTFN +MRM++G              
Sbjct: 123  SGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQ 182

Query: 794  EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADAFLQELVEELRD 973
            E  QF++++SE++ L  A+NK                K+LK +  + DAFL+ L+EE R 
Sbjct: 183  EASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRT 242

Query: 974  GKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEWAISALLNHGEI 1153
             K   N M+ HLL LQ SQPEYY+DQIIKGL   M L GTD+SAVT+EW++S LLNH E+
Sbjct: 243  KKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEV 302

Query: 1154 LKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPRYSSEEFNIGGF 1333
            LKK KDEL+T VG DR+VDESD+P L YL+N+I+ET+RLY+PAPLLLP  +S+E NIGG+
Sbjct: 303  LKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGY 362

Query: 1334 TVPRETIVLINAWAIHR 1384
             VP++TIVLINAWAIHR
Sbjct: 363  KVPQDTIVLINAWAIHR 379


>ref|XP_003611658.1| cytochrome P450 family 81 protein [Medicago truncatula]
 gb|AES94616.1| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 499

 Score =  438 bits (1126), Expect = e-147
 Identities = 217/388 (55%), Positives = 282/388 (72%), Gaps = 12/388 (3%)
 Frame = +2

Query: 257  FILITFKLLLERKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGS 436
            F++IT K+     R FKNLPP P  LPIIGNLH +  PLH + + LS KYG IF LW GS
Sbjct: 14   FLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGS 73

Query: 437  RRVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITL 616
            R VVV+SS T+ +ECFTK DIVL+NRPHFLTGKY+ Y+ T++  +PYGDH+RNLRRI+++
Sbjct: 74   RLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSI 133

Query: 617  DVLSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAG--- 787
            ++LS+HRLNSF +IRR   M L+ KLA  +  GFT VELR   ++MTFNT+MRMV+G   
Sbjct: 134  EILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRY 193

Query: 788  -----DV----EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADA 940
                 DV    E + F+ II E++ L  A+N                 K+LK + KR DA
Sbjct: 194  YGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDA 253

Query: 941  FLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEW 1120
            FLQ L++E R GK + N M+ HLLT Q+SQPEYY+DQIIKGL+  M L GTDTS+VTIEW
Sbjct: 254  FLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSSVTIEW 313

Query: 1121 AISALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPR 1300
            A+S LLNH EI+KKAK+EL+T +GHDR VDE DI  LPYLQ+I++ET+RL++ APLL+P 
Sbjct: 314  AMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPH 373

Query: 1301 YSSEEFNIGGFTVPRETIVLINAWAIHR 1384
             SSE+F++GG+ +P+ TI+++NAW IHR
Sbjct: 374  LSSEDFSLGGYNIPQNTILMVNAWVIHR 401


>sp|Q6WNQ8.1|C81E8_MEDTR RecName: Full=Cytochrome P450 81E8
 gb|AAQ20042.1| CYP81E8 [Medicago truncatula]
          Length = 499

 Score =  438 bits (1126), Expect = e-147
 Identities = 217/388 (55%), Positives = 282/388 (72%), Gaps = 12/388 (3%)
 Frame = +2

Query: 257  FILITFKLLLERKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGS 436
            F++IT K+     R FKNLPP P  LPIIGNLH +  PLH + + LS KYG IF LW GS
Sbjct: 14   FLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGS 73

Query: 437  RRVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITL 616
            R VVV+SS T+ +ECFTK DIVL+NRPHFLTGKY+ Y+ T++  +PYGDH+RNLRRI+++
Sbjct: 74   RLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSI 133

Query: 617  DVLSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAG--- 787
            ++LS+HRLNSF +IRR   M L+ KLA  +  GFT VELR   ++MTFNT+MRMV+G   
Sbjct: 134  EILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRY 193

Query: 788  -----DV----EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADA 940
                 DV    E + F+ II E++ L  A+N                 K+LK + KR DA
Sbjct: 194  YGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDA 253

Query: 941  FLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEW 1120
            FLQ L++E R GK + N M+ HLLT Q+SQPEYY+DQIIKGL+  M L GTDTS+VTIEW
Sbjct: 254  FLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSSVTIEW 313

Query: 1121 AISALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPR 1300
            A+S LLNH EI+KKAK+EL+T +GHDR VDE DI  LPYLQ+I++ET+RL++ APLL+P 
Sbjct: 314  AMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPH 373

Query: 1301 YSSEEFNIGGFTVPRETIVLINAWAIHR 1384
             SSE+F++GG+ +P+ TI+++NAW IHR
Sbjct: 374  LSSEDFSLGGYNIPQNTILMVNAWVIHR 401


>sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase; AltName: Full=CYP GE-3;
            AltName: Full=Cytochrome P450 81E1; AltName:
            Full=Cytochrome P450 91A4
 dbj|BAA22422.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  437 bits (1124), Expect = e-147
 Identities = 215/377 (57%), Positives = 273/377 (72%), Gaps = 12/377 (3%)
 Frame = +2

Query: 290  RKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSRRVVVISSPTL 469
            R R FKNLPP PP+LPIIGNLH++  PLHR+   LS KYG++F LW GSR VVV+SS + 
Sbjct: 25   RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASE 84

Query: 470  VKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLDVLSTHRLNSF 649
             ++CFTK D+VL+NRP FL+GKY+FY+YT+L S  YG+H+RNLRRI  LDVLS HR+NSF
Sbjct: 85   FQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSF 144

Query: 650  SDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAGD------------V 793
            S IRR  T  L+T+LA D+   F  +EL  RL DMTFN +MRM++G              
Sbjct: 145  SGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQ 204

Query: 794  EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADAFLQELVEELRD 973
            E  QF++++SE++ L  A+NK                K+LK +  + DAFL+ L+EE R 
Sbjct: 205  EASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRT 264

Query: 974  GKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEWAISALLNHGEI 1153
             K   N M+ HLL LQ SQPEYY+DQIIKGL   M L GTD+SAVT+EW++S LLNH E+
Sbjct: 265  KKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEV 324

Query: 1154 LKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPRYSSEEFNIGGF 1333
            LKK KDEL+T VG DR+VDESD+P L YL+N+I+ET+RLY+PAPLLLP  +S+E NIGG+
Sbjct: 325  LKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGY 384

Query: 1334 TVPRETIVLINAWAIHR 1384
             VP++TIVLINAWAIHR
Sbjct: 385  KVPQDTIVLINAWAIHR 401


>ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
          Length = 500

 Score =  435 bits (1119), Expect = e-146
 Identities = 220/390 (56%), Positives = 285/390 (73%), Gaps = 16/390 (4%)
 Frame = +2

Query: 263  LITFKLLLE-RKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSR 439
            +IT K++++ + R  KNLPP PPT+PIIGNLH +  P+H +   LS KYG++  LW GSR
Sbjct: 15   IITIKIIIQIQSRKLKNLPPGPPTIPIIGNLHQLKHPMHHTFTTLSQKYGDVLSLWFGSR 74

Query: 440  RVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLD 619
             VVV+SSP+LV+ECF K DIVL+NRP  L GKY+FY+YT+L S+ YGDH+RNLRRI  +D
Sbjct: 75   LVVVVSSPSLVQECFNKNDIVLANRPQLLAGKYIFYNYTTLGSSSYGDHWRNLRRITAID 134

Query: 620  VLSTHRLNSFSDIRRAGTMELVTKLASDTC--EGFTRVELRKRLTDMTFNTMMRMVAG-- 787
            VLS +RLNSF  +R   T  L+ KL +DT   +GFT+VELR RLT+MTFN MM M++G  
Sbjct: 135  VLSNNRLNSFLGVRTDETNRLIQKLLNDTVSDQGFTKVELRHRLTEMTFNGMMMMISGKR 194

Query: 788  ---------DVE-GKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRAD 937
                     DVE  KQF++IISE++ L  A NK+               K+ K + KRAD
Sbjct: 195  YYGDDRDVTDVEEAKQFRDIISEIMSLLGA-NKSEFLPFLRLFDLDDLTKRYKKIAKRAD 253

Query: 938  AFLQELVEELRDG-KSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTI 1114
            AFLQ L+EE + G +S+G+ M+ HLL L+KSQPEYYSD +IKGLIQ M L GTDTSAVTI
Sbjct: 254  AFLQGLIEEHQSGNQSNGDTMIDHLLNLRKSQPEYYSDHMIKGLIQAMLLAGTDTSAVTI 313

Query: 1115 EWAISALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLL 1294
            EW +S LLNH ++L KAK+E+ET +G D++++E D+  LPYLQNII+ET+RL+ PAPL L
Sbjct: 314  EWVMSELLNHPDVLNKAKEEIETQIGKDKLIEEHDLSKLPYLQNIINETLRLHPPAPLSL 373

Query: 1295 PRYSSEEFNIGGFTVPRETIVLINAWAIHR 1384
            P YSSE+F +GGF VP++TI+L N WAIHR
Sbjct: 374  PHYSSEDFTLGGFNVPKDTIILTNVWAIHR 403


>ref|XP_003548043.3| PREDICTED: cytochrome P450 81E8 [Glycine max]
 gb|KHN13402.1| Isoflavone 2'-hydroxylase [Glycine soja]
 gb|KRH08433.1| hypothetical protein GLYMA_16G149300 [Glycine max]
          Length = 498

 Score =  434 bits (1117), Expect = e-145
 Identities = 223/389 (57%), Positives = 281/389 (72%), Gaps = 12/389 (3%)
 Frame = +2

Query: 254  AFILITFKLLLERKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLG 433
            AFIL      L + R FKNLPP P + PIIGNLH +  PLHR+ + LS KYG IF LW G
Sbjct: 10   AFILFLSINFLIQTRRFKNLPPGPFSFPIIGNLHQLKQPLHRTFHALSQKYGPIFSLWFG 69

Query: 434  SRRVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIIT 613
            SR VVV+SSP  V+ECFTK DIVL+NRPHFLTGKY+ Y+ T++  +PYGDH+RNLRRI+ 
Sbjct: 70   SRFVVVVSSPLAVQECFTKNDIVLANRPHFLTGKYIGYNNTTVAVSPYGDHWRNLRRIMA 129

Query: 614  LDVLSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAG-- 787
            L+VLSTHR+NSF + RR   M LV KLA D+  GFT+VEL+ R ++MTFNT+MRMV+G  
Sbjct: 130  LEVLSTHRINSFLENRRDEIMRLVQKLARDSRNGFTKVELKSRFSEMTFNTIMRMVSGKR 189

Query: 788  ------DV----EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRAD 937
                  DV    E +QF+EII E++ L  A+N                 K+LK + KR D
Sbjct: 190  YYGEDCDVSDVQEARQFREIIKELVTLGGANNPGDFLALLRWFDFDGLEKRLKRISKRTD 249

Query: 938  AFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIE 1117
            AFLQ L+++ R+GK   N M+ HLL  Q+SQPEYY+DQIIKGL   M L GTDTSAVT+E
Sbjct: 250  AFLQGLIDQHRNGKHRANTMIDHLLAQQQSQPEYYTDQIIKGLALVMLLAGTDTSAVTLE 309

Query: 1118 WAISALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLP 1297
            WA+S LLNH EILKKAK+EL+T +G DR+VDE DIP LPYLQ+I++ET+RL+  AP+L+P
Sbjct: 310  WAMSNLLNHPEILKKAKNELDTHIGQDRLVDEPDIPKLPYLQSIVYETLRLHPAAPMLVP 369

Query: 1298 RYSSEEFNIGGFTVPRETIVLINAWAIHR 1384
              SSE+  IG + +P+ TI+L+NAWAIHR
Sbjct: 370  HLSSEDCTIGEYNIPQNTILLVNAWAIHR 398


>gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]
 gb|ATY39977.1| Isoflavone 3'-hydroxylase [Astragalus membranaceus]
          Length = 505

 Score =  433 bits (1114), Expect = e-145
 Identities = 223/397 (56%), Positives = 287/397 (72%), Gaps = 20/397 (5%)
 Frame = +2

Query: 254  AFILITFKLLLE-RKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWL 430
            AFIL T K++L+ + R  KNLPP PPT+PIIGNLH++  PLHR+   LS KYG+I  LW 
Sbjct: 13   AFIL-TVKIILQIQSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQKYGDIISLWF 71

Query: 431  GSRRVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRII 610
            GSR VVV++SP++ +ECFTK D+VL+NRP FLTGKY+FY+YT+L SA YGDH+RNLRRI 
Sbjct: 72   GSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRIT 131

Query: 611  TLDVLSTHRLNSFSDIRRAGTMELVTKLASDTCE----GFTRVELRKRLTDMTFNTMMRM 778
            T+DVLS HRLNSF  +R+  T+ LV KL +D  +     FT VELR RLT+MTFN MMRM
Sbjct: 132  TIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRM 191

Query: 779  VA--------GDV----EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCV 922
            ++        GDV    E KQF++IISE++ L  A+NK                K+ K +
Sbjct: 192  ISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGDFLPLLRLFDLDHLEKRCKRI 251

Query: 923  GKRADAFLQELVEELRDGKSS---GNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGT 1093
             KRADAFLQ L+E+ ++   S   G+ M+ HLL L+++QPEYYSD +IKGLIQ M L GT
Sbjct: 252  SKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGT 311

Query: 1094 DTSAVTIEWAISALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLY 1273
            DTSAVTIEW ++ LLN+ E+LKKAK E+ET +G +R+V+E D+  LPYLQN+I E +RL+
Sbjct: 312  DTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRLH 371

Query: 1274 SPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 1384
             PAPLLLP  +SE+  IGGF VP++TI+L N WAIHR
Sbjct: 372  PPAPLLLPHSASEDCTIGGFNVPKDTIILTNIWAIHR 408


>dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  432 bits (1110), Expect = e-144
 Identities = 212/377 (56%), Positives = 271/377 (71%), Gaps = 12/377 (3%)
 Frame = +2

Query: 290  RKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSRRVVVISSPTL 469
            R R FKNLPP PP+LPIIGNLH++  PLHR+   LS KYG++  LW GSR VVV+SS + 
Sbjct: 25   RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVISLWFGSRLVVVVSSASE 84

Query: 470  VKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLDVLSTHRLNSF 649
             ++CFTK D+VL+NRP FL+GKY+FY+YT+L S  YG+H+RNLRRI  LDVLS HR+NSF
Sbjct: 85   FQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSF 144

Query: 650  SDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAGD------------V 793
            S IRR  T  L+T+LA D+   F  +EL  R  DMTFN +MRM++G              
Sbjct: 145  SGIRRDETQRLITRLADDSSTNFAEIELSYRFYDMTFNNIMRMISGKRYYGEDCDMSDLQ 204

Query: 794  EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADAFLQELVEELRD 973
            E  QF++++SE++ L  A+NK                K+LK +  + DAFL+ L++E R 
Sbjct: 205  EASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIQEHRA 264

Query: 974  GKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEWAISALLNHGEI 1153
             K   N M+ HLL LQ SQPEYY+DQIIKGL   M L GTD+SAVT+EW++S LLNH E+
Sbjct: 265  KKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEV 324

Query: 1154 LKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPRYSSEEFNIGGF 1333
            LKK KDEL+T VG DR+VDESD+P L YL+N+I+ET+RLY+PAPLLLP  +S+E NIGG+
Sbjct: 325  LKKVKDELDTHVGQDRLVDESDLPKLSYLKNVINETLRLYTPAPLLLPHSTSDECNIGGY 384

Query: 1334 TVPRETIVLINAWAIHR 1384
             VP++TIVLINAWAIHR
Sbjct: 385  KVPQDTIVLINAWAIHR 401


>ref|XP_016197998.1| isoflavone 3'-hydroxylase [Arachis ipaensis]
          Length = 529

 Score =  430 bits (1105), Expect = e-143
 Identities = 225/422 (53%), Positives = 287/422 (68%), Gaps = 17/422 (4%)
 Frame = +2

Query: 170  TIGSSEAYSKQSLYYIMAXXXXXXXXXXAFILITFKLLLE-RKRSFKNLPPSPPTLPIIG 346
            T   ++  S+ S+   MA             +IT KL    + R FKNLPP PPTLP +G
Sbjct: 10   THSHTQTKSRVSVSLKMATSIYYYLLLTLSFIITLKLFFRFQSRRFKNLPPGPPTLPFLG 69

Query: 347  NLHNITLPLHRSLNNLSHKYGNIFHLWLGSRRVVVISSPTLVKECFTKYDIVLSNRPHFL 526
            NLH +  P+HR    L+  YG+I  LW G+R VVV+SSP+L  ECFTK D+VL+NRP FL
Sbjct: 70   NLHYLKPPVHRIFAGLAASYGDIMSLWFGNRLVVVVSSPSLAHECFTKNDVVLANRPRFL 129

Query: 527  TGKYLFYDYTSLDSAPYGDHFRNLRRIITLDVLSTHRLNSFSDIRRAGTMELVTKLASDT 706
            TGKY+FY+YT+L SA YGDH+RNLRRI T+DVLS+HRLNSF D+R+  T  L+ KL  DT
Sbjct: 130  TGKYIFYNYTTLGSASYGDHWRNLRRITTVDVLSSHRLNSFLDVRKDETKRLIEKLWKDT 189

Query: 707  CEG-FTRVELRKRLTDMTFNTMMRMVAG------DV------EGKQFKEIISEMIPLFSA 847
              G F +VELR RLT+MTFN MMRM++G      DV      E KQF+EII+E++ L  A
Sbjct: 190  RNGEFVKVELRSRLTEMTFNGMMRMISGKRYYGDDVEMNDVEEAKQFREIITEILSLLGA 249

Query: 848  SNKAXXXXXXXXXXXXXXVKKLKCVGKRADAFLQELVEELR---DGKSSGNAMLHHLLTL 1018
            SNK                K+LK + KRAD+FLQ L++E R      +  + M+ HLL L
Sbjct: 250  SNKGDFMPLAKFFDFDHLEKRLKNIAKRADSFLQGLIQEHRVAAGSNADSDTMIFHLLKL 309

Query: 1019 QKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEWAISALLNHGEILKKAKDELETVVGHD 1198
            Q++QPEYYSD +IKGLIQ M L GTDTSAV +EWA++ LLNH EI+KK KDEL+T +G D
Sbjct: 310  QETQPEYYSDLMIKGLIQAMLLAGTDTSAVAMEWAMAELLNHPEIMKKVKDELDTQIGKD 369

Query: 1199 RMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAI 1378
            R+V+E D+  LPYLQN+I ET+RL+ PAPLLLP  +SE+  IGG+ +P++TIVL N W I
Sbjct: 370  RLVEEQDLSKLPYLQNVISETLRLHPPAPLLLPHSASEDCTIGGYNIPKDTIVLTNVWLI 429

Query: 1379 HR 1384
            HR
Sbjct: 430  HR 431


>ref|XP_016193629.1| isoflavone 3'-hydroxylase [Arachis ipaensis]
          Length = 508

 Score =  429 bits (1103), Expect = e-143
 Identities = 221/390 (56%), Positives = 276/390 (70%), Gaps = 15/390 (3%)
 Frame = +2

Query: 260  ILITFKLLLERKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSR 439
            I+IT         + KNLPP PPTLPIIGNLH++   LHR+ + LS  YG+IF LW GSR
Sbjct: 18   IIITLTKTNTSHSNKKNLPPCPPTLPIIGNLHHLKPRLHRTFHALSQTYGHIFSLWFGSR 77

Query: 440  RVVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLD 619
             VVV+S PTL ++C TKYD VL+NRP FLTGKYLFY++TSL  +   DH+RNLRRI T+D
Sbjct: 78   LVVVVSCPTLAQQCLTKYDTVLANRPRFLTGKYLFYNHTSLVFSSNNDHWRNLRRIATMD 137

Query: 620  VLSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAG---- 787
            VLST RLNSF +IRR      +  L +DT  GF RV+LR R+T+M+ N MMRMV+G    
Sbjct: 138  VLSTPRLNSFFEIRRDEVTRFIRNLVADTSMGFARVQLRPRITEMSLNNMMRMVSGKRNE 197

Query: 788  ----------DVEGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRAD 937
                      D    +F+EII E++ L  A+NK+              VK+LK VG+ AD
Sbjct: 198  YRKDDCNARDDNGAGRFREIIREILSLVDANNKSDFLPLLRWFDFDGFVKRLKRVGEEAD 257

Query: 938  AFLQELVEELRDGKSSGN-AMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTI 1114
             F Q L+EE R+G+   N  M+ HLL LQK QP YY+D IIKGLIQ M L GT+T+ +T+
Sbjct: 258  MFWQGLLEEHRNGEHGNNDTMIQHLLLLQKLQPNYYTDHIIKGLIQDMLLAGTNTTVLTM 317

Query: 1115 EWAISALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLL 1294
            EW +SALLNH EIL+KAKDE++  +G DR+VDESDIP LPYLQNIIHET+RLYSP+PLLL
Sbjct: 318  EWTLSALLNHPEILRKAKDEIDNHIGKDRLVDESDIPKLPYLQNIIHETLRLYSPSPLLL 377

Query: 1295 PRYSSEEFNIGGFTVPRETIVLINAWAIHR 1384
            P +SS+E NIGGFT+PR+TIVLIN+WA+ R
Sbjct: 378  PHFSSDEVNIGGFTIPRDTIVLINSWAMRR 407


>ref|XP_016201681.2| cytochrome P450 81E8 [Arachis ipaensis]
          Length = 499

 Score =  428 bits (1101), Expect = e-143
 Identities = 214/386 (55%), Positives = 280/386 (72%), Gaps = 12/386 (3%)
 Frame = +2

Query: 263  LITFKLLLERKRSFKNLPPSPPTLPIIGNLHNITLPLHRSLNNLSHKYGNIFHLWLGSRR 442
            L+T KLL  + R+ KNLPP PP LP+IGNLH++  PLHR    LS KYG++F LW GSR 
Sbjct: 16   LLTLKLLF-KSRALKNLPPGPPYLPLIGNLHHLKKPLHRHFRGLSEKYGDVFSLWFGSRL 74

Query: 443  VVVISSPTLVKECFTKYDIVLSNRPHFLTGKYLFYDYTSLDSAPYGDHFRNLRRIITLDV 622
            VVV+SSPT+V+ECFTK D+VL+NRP FL+GKY+FY+YT++ S+ YG+H+RNLRRI TL+V
Sbjct: 75   VVVVSSPTVVQECFTKNDVVLANRPRFLSGKYIFYNYTTVGSSEYGEHWRNLRRIATLEV 134

Query: 623  LSTHRLNSFSDIRRAGTMELVTKLASDTCEGFTRVELRKRLTDMTFNTMMRMVAG----- 787
            LSTHR+NSF +IRR   M LV KLA +   GF +VELR +L++MTFNT+MRMV+G     
Sbjct: 135  LSTHRINSFLEIRRDEIMRLVQKLAQECSNGFNKVELRSKLSEMTFNTIMRMVSGKRYYG 194

Query: 788  ---DV----EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXXVKKLKCVGKRADAFL 946
               DV    E ++F+ I  E++ L  A+N                  KLK +  R D+FL
Sbjct: 195  EDCDVTDVEEARRFRAITKELVALGGANNPGDFISVLRWFDFDGLENKLKRISNRTDSFL 254

Query: 947  QELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTDTSAVTIEWAI 1126
            Q L++E R+GK + N M+ HLLT Q+S PEYY+DQIIKGLI  M L GTDTS+VT+EWA+
Sbjct: 255  QGLIDEHRNGKRNTNTMIDHLLTQQQSHPEYYTDQIIKGLIMVMLLAGTDTSSVTLEWAM 314

Query: 1127 SALLNHGEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYSPAPLLLPRYS 1306
            S LLNH EIL++A+ E++T +G D ++DESDI  L YLQNI++ET RL+  APLL+P  S
Sbjct: 315  SNLLNHPEILERARKEIDTEIGQDHLIDESDISKLNYLQNIVYETFRLHPAAPLLVPHLS 374

Query: 1307 SEEFNIGGFTVPRETIVLINAWAIHR 1384
            SE+  + G+ VP+ TIVL+NAWAIHR
Sbjct: 375  SEDCTLEGYKVPQNTIVLVNAWAIHR 400