BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus24_contig00012362
         (774 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arieti...   112   3e-24
gb|PNY06816.1| DNA topoisomerase 2-like protein [Trifolium prate...   105   6e-22
ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago tru...   104   1e-21
gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium prate...   103   4e-21
dbj|GAU12625.1| hypothetical protein TSUD_121160 [Trifolium subt...   102   9e-21
dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subt...   102   9e-21
dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subt...   100   2e-20
ref|XP_003545986.1| PREDICTED: DNA topoisomerase 2-like [Glycine...   100   2e-20
gb|KYP71391.1| DNA topoisomerase 2 [Cajanus cajan]                    100   4e-20
ref|XP_020212372.1| DNA topoisomerase 2 [Cajanus cajan]               100   4e-20
ref|XP_003601827.1| DNA topoisomerase IV subunit A [Medicago tru...    99   1e-19
gb|KHN45085.1| DNA topoisomerase 2, partial [Glycine soja]             99   1e-19
sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName:...    99   1e-19
dbj|GAU30192.1| hypothetical protein TSUD_311430 [Trifolium subt...    98   2e-19
gb|KRH32967.1| hypothetical protein GLYMA_10G090000 [Glycine max]      98   2e-19
ref|XP_014618555.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisom...    98   2e-19
ref|XP_014501355.1| DNA topoisomerase 2 [Vigna radiata var. radi...    95   2e-18
ref|XP_017425524.1| PREDICTED: DNA topoisomerase 2 [Vigna angula...    95   3e-18
ref|XP_007148908.1| hypothetical protein PHAVU_005G024200g [Phas...    84   1e-14
gb|EXB68160.1| DNA topoisomerase 2 [Morus notabilis]                   82   8e-14

>ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
 ref|XP_004517303.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
          Length = 1472

 Score =  112 bits (279), Expect = 3e-24
 Identities = 58/90 (64%), Positives = 71/90 (78%), Gaps = 1/90 (1%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSADDDILSLEEHLAAYNFESSSENTGGM 625
            E E++N+SME+EN VEV KP GRA  K TQK++ DDDILSL+E LAAYNFESS E +  M
Sbjct: 1206 EPENDNSSMEIENPVEVAKPKGRAVSKNTQKET-DDDILSLQERLAAYNFESSGEKSAAM 1264

Query: 626  ETEEPSLPTGKKQNNRKGGAKKK-TTVVLD 712
            ETEEP++P  KK  NR+GG KKK +T+VLD
Sbjct: 1265 ETEEPAVPAAKKPINRRGGVKKKSSTIVLD 1294


>gb|PNY06816.1| DNA topoisomerase 2-like protein [Trifolium pratense]
          Length = 1254

 Score =  105 bits (262), Expect = 6e-22
 Identities = 57/94 (60%), Positives = 71/94 (75%), Gaps = 2/94 (2%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSADD-DILSLEEHLAAYNFESSSENTGG 622
            E E++N+SMEVEN VEV KP GR G K TQK + D+ DI SL+E LAAYNFESS E +G 
Sbjct: 1020 EPENDNSSMEVENAVEVVKPKGRVGSKNTQKKAGDESDIPSLQERLAAYNFESSGEKSGA 1079

Query: 623  METEEPSLPTGKKQNNRKGGAKK-KTTVVLDTDS 721
            ME++EP +P GKKQ N +G AKK  +T+VL +DS
Sbjct: 1080 MESDEP-VPAGKKQINERGDAKKMSSTIVLGSDS 1112


>ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago truncatula]
 gb|AES69522.2| DNA topoisomerase IV subunit A [Medicago truncatula]
          Length = 1504

 Score =  104 bits (260), Expect = 1e-21
 Identities = 56/99 (56%), Positives = 70/99 (70%), Gaps = 7/99 (7%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKP------NGRAGPKKTQKDSADDDILSLEEHLAAYNFESSS 607
            E E++N+SME+EN VEV KP       GRAG KK  +  ADD+ILSL+E LA YN ESS 
Sbjct: 1204 EPENDNSSMEIENAVEVVKPVEVAKPKGRAGSKKNAQTEADDEILSLQERLAVYNLESSG 1263

Query: 608  ENTGGMETEEPSLPTGKKQNNRKGGAKKKT-TVVLDTDS 721
            E +  ME E P  P GKKQ N++GGAKKK+ T+VL++DS
Sbjct: 1264 EKSQAMEEEPPVPPAGKKQINKRGGAKKKSNTIVLESDS 1302


>gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium pratense]
          Length = 1492

 Score =  103 bits (256), Expect = 4e-21
 Identities = 53/93 (56%), Positives = 70/93 (75%), Gaps = 1/93 (1%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSADDDILSLEEHLAAYNFESSSENTGGM 625
            E E++N+SME+EN V++ KP GRAG KK      D+++LSL+E LAAYN ESS E +  M
Sbjct: 1234 EPENDNSSMEIENAVDIAKPKGRAGSKKNTPKEPDEEMLSLQERLAAYNIESSGEKSQAM 1293

Query: 626  ETEEPSLPTGKKQNNRKGGAKKK-TTVVLDTDS 721
            E+EEP +P  KKQ NR GGAKKK +T+VLD+D+
Sbjct: 1294 ESEEP-VPAMKKQINRGGGAKKKSSTIVLDSDN 1325


>dbj|GAU12625.1| hypothetical protein TSUD_121160 [Trifolium subterraneum]
          Length = 1315

 Score =  102 bits (253), Expect = 9e-21
 Identities = 53/93 (56%), Positives = 70/93 (75%), Gaps = 1/93 (1%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSADDDILSLEEHLAAYNFESSSENTGGM 625
            E E+ N+SME+EN V++ KP GRAG KK      +++ILSL+E LAAYN ESS E +  M
Sbjct: 1051 EPENYNSSMEIENAVDIAKPKGRAGSKKNTPKEPEEEILSLQERLAAYNIESSGEKSQAM 1110

Query: 626  ETEEPSLPTGKKQNNRKGGAKKKT-TVVLDTDS 721
            E+EEP +P  KKQ NR+GGAKKK+ T+VL++DS
Sbjct: 1111 ESEEP-VPDMKKQINRRGGAKKKSNTIVLESDS 1142


>dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subterraneum]
          Length = 1359

 Score =  102 bits (253), Expect = 9e-21
 Identities = 53/93 (56%), Positives = 70/93 (75%), Gaps = 1/93 (1%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSADDDILSLEEHLAAYNFESSSENTGGM 625
            E E+ N+SME+EN V++ KP GRAG KK      +++ILSL+E LAAYN ESS E +  M
Sbjct: 1095 EPENYNSSMEIENAVDIAKPKGRAGSKKNTPKEPEEEILSLQERLAAYNIESSGEKSQAM 1154

Query: 626  ETEEPSLPTGKKQNNRKGGAKKKT-TVVLDTDS 721
            E+EEP +P  KKQ NR+GGAKKK+ T+VL++DS
Sbjct: 1155 ESEEP-VPDMKKQINRRGGAKKKSNTIVLESDS 1186


>dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subterraneum]
          Length = 1336

 Score =  100 bits (250), Expect = 2e-20
 Identities = 54/83 (65%), Positives = 66/83 (79%), Gaps = 2/83 (2%)
 Frame = +2

Query: 479  ENVVEVTKPNGRAGPKKTQKDSADD-DILSLEEHLAAYNFESSSENTGGMETEEPSLPTG 655
            ENVVEV KP GRAG K TQK + D+ DILSL+E LAAYNFESS E +G ME+EEP +P G
Sbjct: 1113 ENVVEVVKPKGRAGSKNTQKKAGDESDILSLQERLAAYNFESSGEKSGAMESEEP-VPAG 1171

Query: 656  KKQNNRKGGAKKK-TTVVLDTDS 721
            KKQ N++ GAKK  +T+VL++DS
Sbjct: 1172 KKQINKRAGAKKMLSTIVLESDS 1194


>ref|XP_003545986.1| PREDICTED: DNA topoisomerase 2-like [Glycine max]
 gb|KRH13973.1| hypothetical protein GLYMA_15G276000 [Glycine max]
          Length = 1484

 Score =  100 bits (250), Expect = 2e-20
 Identities = 53/95 (55%), Positives = 70/95 (73%), Gaps = 3/95 (3%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDS--ADDDILSLEEHLAAYNFESSSENTG 619
            E E++N+SME+ENV EV KP GRAG K TQ     A+D+I+SL+E LAAYNF +SS+ + 
Sbjct: 1214 EPENDNSSMEIENVAEVAKPKGRAGSKNTQNTQPVAEDEIVSLQERLAAYNFGASSDISE 1273

Query: 620  GMETEEPSLPTGKKQN-NRKGGAKKKTTVVLDTDS 721
            GMETEEPS P  KK+   R+G AKKK++ ++  DS
Sbjct: 1274 GMETEEPSAPAEKKEGPKRRGAAKKKSSAIVLDDS 1308


>gb|KYP71391.1| DNA topoisomerase 2 [Cajanus cajan]
          Length = 1446

 Score =  100 bits (248), Expect = 4e-20
 Identities = 55/97 (56%), Positives = 69/97 (71%), Gaps = 5/97 (5%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKK----TQKDSADDDILSLEEHLAAYNFESSSEN 613
            E E++N+ MEVENV EV KP GRAG K     TQ   ADD+ILSL+E LAAYNF +SS+ 
Sbjct: 1174 EPENDNSPMEVENVAEVPKPKGRAGSKNSLSSTQTTVADDEILSLQERLAAYNFGASSDI 1233

Query: 614  TGGMETEEPSLPTGKKQ-NNRKGGAKKKTTVVLDTDS 721
            + GMETEEP  P GKK+   R+G AKKK++ ++  DS
Sbjct: 1234 SAGMETEEPPAPAGKKEAPKRRGPAKKKSSAIVLDDS 1270


>ref|XP_020212372.1| DNA topoisomerase 2 [Cajanus cajan]
          Length = 1480

 Score =  100 bits (248), Expect = 4e-20
 Identities = 55/97 (56%), Positives = 69/97 (71%), Gaps = 5/97 (5%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKK----TQKDSADDDILSLEEHLAAYNFESSSEN 613
            E E++N+ MEVENV EV KP GRAG K     TQ   ADD+ILSL+E LAAYNF +SS+ 
Sbjct: 1208 EPENDNSPMEVENVAEVPKPKGRAGSKNSLSSTQTTVADDEILSLQERLAAYNFGASSDI 1267

Query: 614  TGGMETEEPSLPTGKKQ-NNRKGGAKKKTTVVLDTDS 721
            + GMETEEP  P GKK+   R+G AKKK++ ++  DS
Sbjct: 1268 SAGMETEEPPAPAGKKEAPKRRGPAKKKSSAIVLDDS 1304


>ref|XP_003601827.1| DNA topoisomerase IV subunit A [Medicago truncatula]
 gb|AES72078.1| DNA topoisomerase IV subunit A [Medicago truncatula]
          Length = 1345

 Score = 99.0 bits (245), Expect = 1e-19
 Identities = 52/93 (55%), Positives = 70/93 (75%), Gaps = 1/93 (1%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSADDDILSLEEHLAAYNFESSSENTGGM 625
            E E++N+SME+EN VEV KP G +  K T++   D  ILS++E LAAYNFES  E +G M
Sbjct: 1137 EPENDNSSMEIENAVEVAKPKGGSVFKNTKEADDDSYILSIQERLAAYNFESPGEKSGAM 1196

Query: 626  ETEEPSLPTGKKQNNRKGGAKKK-TTVVLDTDS 721
            E+EEP +P GKKQ N++GGAKKK +T+ L++DS
Sbjct: 1197 ESEEP-VPAGKKQINKRGGAKKKSSTIGLESDS 1228


>gb|KHN45085.1| DNA topoisomerase 2, partial [Glycine soja]
          Length = 1379

 Score = 99.0 bits (245), Expect = 1e-19
 Identities = 52/95 (54%), Positives = 69/95 (72%), Gaps = 3/95 (3%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDS--ADDDILSLEEHLAAYNFESSSENTG 619
            E E++N+SME+ENV EV KP GRAG K TQ     A+D+I+SL+E LAAYNF +SS+ + 
Sbjct: 1109 EPENDNSSMEIENVAEVAKPKGRAGSKNTQNTQPVAEDEIVSLQERLAAYNFGASSDISE 1168

Query: 620  GMETEEPSLPTGKKQN-NRKGGAKKKTTVVLDTDS 721
            GMETEEP  P  KK+   R+G AKKK++ ++  DS
Sbjct: 1169 GMETEEPPAPAEKKEGPKRRGAAKKKSSAIVLDDS 1203


>sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase
            II; AltName: Full=PsTopII
 emb|CAA74891.1| topoisomerase II [Pisum sativum]
          Length = 1462

 Score = 99.0 bits (245), Expect = 1e-19
 Identities = 55/100 (55%), Positives = 70/100 (70%), Gaps = 8/100 (8%)
 Frame = +2

Query: 446  EYEDENASMEVENVVE------VTKPNGRAGPKKTQKDSADDDILSLEEHLAAYNFESSS 607
            E E++N+SME+EN VE      V KP GRA PKK  +   +DDI SL+E LAAYN ESS 
Sbjct: 1185 EPENDNSSMEIENAVEAAKPAEVAKPKGRAAPKKNIQKEPEDDIQSLQERLAAYNIESSG 1244

Query: 608  ENTGGMETEE-PSLPTGKKQNNRKGGAKKK-TTVVLDTDS 721
            E +  ME+EE      GKKQNN++GGAKKK +T+VL++DS
Sbjct: 1245 EKSQAMESEEVQQKAAGKKQNNKRGGAKKKSSTIVLESDS 1284


>dbj|GAU30192.1| hypothetical protein TSUD_311430 [Trifolium subterraneum]
          Length = 1252

 Score = 98.2 bits (243), Expect = 2e-19
 Identities = 54/95 (56%), Positives = 69/95 (72%), Gaps = 3/95 (3%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSADDDIL--SLEEHLAAYNFESSSENTG 619
            E E++ +SMEVENVVEV KP GRAG K TQK + D++ +  SL E LA YNFESS E + 
Sbjct: 1021 EPENDKSSMEVENVVEVVKPKGRAGSKNTQKKAGDENDIPSSLHERLAVYNFESSGEKSE 1080

Query: 620  GMETEEPSLPTGKKQNNRKGGAKK-KTTVVLDTDS 721
             ME+EEP +P GKK  N++ GAKK  +T+VL+ DS
Sbjct: 1081 AMESEEP-VPAGKKPINKRAGAKKMSSTIVLEPDS 1114


>gb|KRH32967.1| hypothetical protein GLYMA_10G090000 [Glycine max]
          Length = 1459

 Score = 98.2 bits (243), Expect = 2e-19
 Identities = 53/97 (54%), Positives = 69/97 (71%), Gaps = 5/97 (5%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSA----DDDILSLEEHLAAYNFESSSEN 613
            E E++N+SMEVENV EV KP GRAG   TQK++     DD+ILSL+E LAAYNF +SS+ 
Sbjct: 1187 EPENDNSSMEVENVAEVVKPKGRAGSNNTQKNTLPVAEDDEILSLQERLAAYNFGASSDI 1246

Query: 614  TGGMETEEPSLPTGKKQ-NNRKGGAKKKTTVVLDTDS 721
            + GMETEEP  P  KK+   R+G AKK ++ ++  DS
Sbjct: 1247 SAGMETEEPPAPAEKKEAPKRRGAAKKMSSAIVLDDS 1283


>ref|XP_014618555.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisomerase 2-like [Glycine
            max]
          Length = 1481

 Score = 98.2 bits (243), Expect = 2e-19
 Identities = 53/97 (54%), Positives = 69/97 (71%), Gaps = 5/97 (5%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSA----DDDILSLEEHLAAYNFESSSEN 613
            E E++N+SMEVENV EV KP GRAG   TQK++     DD+ILSL+E LAAYNF +SS+ 
Sbjct: 1209 EPENDNSSMEVENVAEVVKPKGRAGSNNTQKNTLPVAEDDEILSLQERLAAYNFGASSDI 1268

Query: 614  TGGMETEEPSLPTGKKQ-NNRKGGAKKKTTVVLDTDS 721
            + GMETEEP  P  KK+   R+G AKK ++ ++  DS
Sbjct: 1269 SAGMETEEPPAPAEKKEAPKRRGAAKKMSSAIVLDDS 1305


>ref|XP_014501355.1| DNA topoisomerase 2 [Vigna radiata var. radiata]
          Length = 1492

 Score = 95.1 bits (235), Expect = 2e-18
 Identities = 52/94 (55%), Positives = 64/94 (68%), Gaps = 2/94 (2%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSADDDILSLEEHLAAYNFESSSENTGGM 625
            E +++N+SMEVENV EVTKP GRA   +  +   DD+I SL+E LAAYNF  SS+ + GM
Sbjct: 1213 EPDNDNSSMEVENVAEVTKPKGRAAGSENTQTVGDDEIASLQERLAAYNFAPSSDQSTGM 1272

Query: 626  ETEEPSLPTGKKQNNRKG-GAKKKTTVVLD-TDS 721
            ETEE   P GK    R+G  AKK T +VLD TDS
Sbjct: 1273 ETEERPAPAGKTAPKRRGPAAKKSTAIVLDETDS 1306


>ref|XP_017425524.1| PREDICTED: DNA topoisomerase 2 [Vigna angularis]
 gb|KOM42453.1| hypothetical protein LR48_Vigan05g005700 [Vigna angularis]
 dbj|BAT93356.1| hypothetical protein VIGAN_07230300 [Vigna angularis var. angularis]
          Length = 1480

 Score = 94.7 bits (234), Expect = 3e-18
 Identities = 53/94 (56%), Positives = 66/94 (70%), Gaps = 2/94 (2%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTKPNGRAGPKKTQKDSADDDILSLEEHLAAYNFESSSENTGGM 625
            E +++++SMEVENV EVTKP GRA   +  +   DD+I SL+E LAAYNF  SS+ + GM
Sbjct: 1212 EPDNDDSSMEVENVAEVTKPKGRAAGSENTQTVGDDEIASLQERLAAYNFAPSSDLSTGM 1271

Query: 626  ETEEPSLPTGKKQNNRKG-GAKKKTTVVL-DTDS 721
            ETEE   PTGK    R+G  AKK +TVVL DTDS
Sbjct: 1272 ETEERPAPTGKTAPKRRGPAAKKSSTVVLDDTDS 1305


>ref|XP_007148908.1| hypothetical protein PHAVU_005G024200g [Phaseolus vulgaris]
 gb|ESW20902.1| hypothetical protein PHAVU_005G024200g [Phaseolus vulgaris]
          Length = 1474

 Score = 84.3 bits (207), Expect = 1e-14
 Identities = 46/92 (50%), Positives = 61/92 (66%), Gaps = 3/92 (3%)
 Frame = +2

Query: 446  EYEDENASMEVENVVEVTK--PNGRAGPKKTQKDSADDDILSLEEHLAAYNFESSSENTG 619
            E E++++SMEVENV +VT   P GR    +  +   +D+I SL+E LAAYNF  SS+ + 
Sbjct: 1213 EPENDSSSMEVENVAQVTTNPPQGRGAGSEYTQTVGEDEIASLQERLAAYNFAPSSDKST 1272

Query: 620  GMETEEPSLPTGKKQNNRKG-GAKKKTTVVLD 712
            GME EEP  P GK    R+G  AKKK+T+VLD
Sbjct: 1273 GMENEEPPAPAGKTVPKRRGPAAKKKSTIVLD 1304


>gb|EXB68160.1| DNA topoisomerase 2 [Morus notabilis]
          Length = 1451

 Score = 81.6 bits (200), Expect = 8e-14
 Identities = 48/119 (40%), Positives = 69/119 (57%), Gaps = 13/119 (10%)
 Frame = +2

Query: 401  RENFTIAASHISIAPEYEDENASMEVENVVEVTKPNGRAGPKKTQK-------DSADDDI 559
            R+N   A++  +IA   E+ +++ME E   EV KP GRAGPKK          D  DD++
Sbjct: 1164 RKNTKKASNAEAIADITENFDSAMEFEKAPEVAKPKGRAGPKKAASKVVVEDDDDDDDEV 1223

Query: 560  LSLEEHLAAYNFESSSENTGGMETEEPSLPTGKKQNNRKGGAKKKT------TVVLDTD 718
            L+L++ LAAYN +SS + +  M+TE P +P  KK   ++  A+KKT      TV LD D
Sbjct: 1224 LALKDRLAAYNLDSSPDKSSEMDTEAPQVPVPKKAPTKRAAAQKKTLATVSETVNLDDD 1282