BLASTX nr result
ID: Astragalus24_contig00009366
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Astragalus24_contig00009366
(325 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004511540.1| PREDICTED: calcium-dependent protein kinase ... 136 1e-35
ref|XP_013453394.1| calmodulin-domain kinase CDPK protein [Medic... 128 4e-33
ref|XP_003611046.1| calmodulin-domain kinase CDPK protein [Medic... 128 1e-32
ref|XP_019424127.1| PREDICTED: calcium-dependent protein kinase ... 127 2e-32
ref|XP_007160124.1| hypothetical protein PHAVU_002G294500g [Phas... 124 4e-31
ref|XP_014507354.1| calcium-dependent protein kinase 1 [Vigna ra... 124 4e-31
gb|OWM85269.1| hypothetical protein CDL15_Pgr028056 [Punica gran... 124 6e-31
gb|KRH59993.1| hypothetical protein GLYMA_05G213200 [Glycine max] 122 1e-30
ref|XP_017442255.1| PREDICTED: calcium-dependent protein kinase ... 122 2e-30
ref|XP_016202154.1| calcium-dependent protein kinase 1 [Arachis ... 122 2e-30
gb|PNX89517.1| calcium-dependent protein kinase 3-like protein [... 117 2e-30
ref|XP_020223591.1| calcium-dependent protein kinase 1 [Cajanus ... 122 2e-30
ref|XP_003525360.1| PREDICTED: calcium-dependent protein kinase ... 122 3e-30
ref|XP_013447139.1| calmodulin-domain kinase CDPK protein [Medic... 120 8e-30
ref|XP_024173288.1| calcium-dependent protein kinase 1 [Rosa chi... 120 1e-29
ref|XP_021838451.1| calcium-dependent protein kinase 1 [Spinacia... 120 1e-29
ref|XP_015388666.1| PREDICTED: calcium-dependent protein kinase ... 114 1e-29
ref|XP_024028142.1| calcium-dependent protein kinase 1 [Morus no... 120 2e-29
gb|OAY59691.1| hypothetical protein MANES_01G050900, partial [Ma... 119 2e-29
gb|KNA21138.1| hypothetical protein SOVF_046030 [Spinacia oleracea] 120 2e-29
>ref|XP_004511540.1| PREDICTED: calcium-dependent protein kinase 3-like [Cicer
arietinum]
Length = 509
Score = 136 bits (342), Expect = 1e-35
Identities = 74/112 (66%), Positives = 80/112 (71%), Gaps = 5/112 (4%)
Frame = +1
Query: 4 SEP--SSHPKPENTTPPTHISVLPPDRNPKQPTST---TSTMGRVLGRPMEDVRSTYIFG 168
SEP +S +P ++ P IS PPD NP QPT T TSTMGRVLGRPMED+RSTY FG
Sbjct: 6 SEPISNSESQPPHSNP---ISTPPPDLNPNQPTPTATPTSTMGRVLGRPMEDIRSTYTFG 62
Query: 169 RELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
RELGRGQFGVTYLVTHK+TKEQFACKSIAT EVQIMHHL
Sbjct: 63 RELGRGQFGVTYLVTHKITKEQFACKSIATRKIINRGDLDDIRREVQIMHHL 114
>ref|XP_013453394.1| calmodulin-domain kinase CDPK protein [Medicago truncatula]
gb|KEH27423.1| calmodulin-domain kinase CDPK protein [Medicago truncatula]
Length = 433
Score = 128 bits (322), Expect = 4e-33
Identities = 68/113 (60%), Positives = 79/113 (69%), Gaps = 7/113 (6%)
Frame = +1
Query: 7 EPSSHPKPENTTPPTH---ISVLPPDRNPKQ----PTSTTSTMGRVLGRPMEDVRSTYIF 165
+PSS + + + P+H S PP+R PKQ PT ++S +GRVLG+PMEDVRSTY F
Sbjct: 10 KPSSDSQTRSKSEPSHSTPTSFHPPNRTPKQSTHTPTPSSSPLGRVLGKPMEDVRSTYTF 69
Query: 166 GRELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
GRELGRGQFGVTYLVTHK+TKEQFACKSIAT EVQIMHHL
Sbjct: 70 GRELGRGQFGVTYLVTHKLTKEQFACKSIATRKLIRRDDLDDIRREVQIMHHL 122
>ref|XP_003611046.1| calmodulin-domain kinase CDPK protein [Medicago truncatula]
gb|AES94004.1| calmodulin-domain kinase CDPK protein [Medicago truncatula]
Length = 517
Score = 128 bits (322), Expect = 1e-32
Identities = 68/113 (60%), Positives = 79/113 (69%), Gaps = 7/113 (6%)
Frame = +1
Query: 7 EPSSHPKPENTTPPTH---ISVLPPDRNPKQ----PTSTTSTMGRVLGRPMEDVRSTYIF 165
+PSS + + + P+H S PP+R PKQ PT ++S +GRVLG+PMEDVRSTY F
Sbjct: 10 KPSSDSQTRSKSEPSHSTPTSFHPPNRTPKQSTHTPTPSSSPLGRVLGKPMEDVRSTYTF 69
Query: 166 GRELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
GRELGRGQFGVTYLVTHK+TKEQFACKSIAT EVQIMHHL
Sbjct: 70 GRELGRGQFGVTYLVTHKLTKEQFACKSIATRKLIRRDDLDDIRREVQIMHHL 122
>ref|XP_019424127.1| PREDICTED: calcium-dependent protein kinase 1 [Lupinus
angustifolius]
gb|OIV92646.1| hypothetical protein TanjilG_17997 [Lupinus angustifolius]
Length = 517
Score = 127 bits (320), Expect = 2e-32
Identities = 71/113 (62%), Positives = 79/113 (69%), Gaps = 5/113 (4%)
Frame = +1
Query: 1 NSEPSSHPKPENTTPPTHISVLPPDRNPKQP-----TSTTSTMGRVLGRPMEDVRSTYIF 165
NSEPS+ P + ISVLPP P P +STTS+MGR+LGRPMEDVRSTY+F
Sbjct: 16 NSEPSN---PNSNG----ISVLPPHSKPSPPKSPLPSSTTSSMGRILGRPMEDVRSTYVF 68
Query: 166 GRELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
GRELGRGQFGVTYLVT+K+TKEQFACKSIAT EVQIMHHL
Sbjct: 69 GRELGRGQFGVTYLVTNKLTKEQFACKSIATRKLIHRDDIDDVCREVQIMHHL 121
>ref|XP_007160124.1| hypothetical protein PHAVU_002G294500g [Phaseolus vulgaris]
gb|ESW32118.1| hypothetical protein PHAVU_002G294500g [Phaseolus vulgaris]
Length = 519
Score = 124 bits (311), Expect = 4e-31
Identities = 64/93 (68%), Positives = 69/93 (74%), Gaps = 3/93 (3%)
Frame = +1
Query: 55 ISVLPPDRNPKQPT---STTSTMGRVLGRPMEDVRSTYIFGRELGRGQFGVTYLVTHKVT 225
I+VLPP+ NP P +S++GRVLGRPMEDVRS YIFGRELGRGQFGVTYLVTHKVT
Sbjct: 29 ITVLPPNTNPSPPLPKPQASSSLGRVLGRPMEDVRSIYIFGRELGRGQFGVTYLVTHKVT 88
Query: 226 KEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
KEQFACKSIAT EVQIMHHL
Sbjct: 89 KEQFACKSIATRKLVNRDDIDDVRREVQIMHHL 121
>ref|XP_014507354.1| calcium-dependent protein kinase 1 [Vigna radiata var. radiata]
Length = 520
Score = 124 bits (311), Expect = 4e-31
Identities = 64/93 (68%), Positives = 69/93 (74%), Gaps = 3/93 (3%)
Frame = +1
Query: 55 ISVLPPDRNPKQPT---STTSTMGRVLGRPMEDVRSTYIFGRELGRGQFGVTYLVTHKVT 225
I+VLPP+ NP P +S++GRVLGRPMEDVRS YIFGRELGRGQFGVTYLVTHKVT
Sbjct: 30 ITVLPPNTNPSPPPPKPQASSSLGRVLGRPMEDVRSIYIFGRELGRGQFGVTYLVTHKVT 89
Query: 226 KEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
KEQFACKSIAT EVQIMHHL
Sbjct: 90 KEQFACKSIATRKLVNRDDIDDIRREVQIMHHL 122
>gb|OWM85269.1| hypothetical protein CDL15_Pgr028056 [Punica granatum]
Length = 531
Score = 124 bits (310), Expect = 6e-31
Identities = 64/108 (59%), Positives = 70/108 (64%)
Frame = +1
Query: 1 NSEPSSHPKPENTTPPTHISVLPPDRNPKQPTSTTSTMGRVLGRPMEDVRSTYIFGRELG 180
N P++ P+P PP PP QP T+GRVLGRPMEDVR+TYIFGRELG
Sbjct: 33 NIHPTTPPRPHPAAPPR-----PPPPRQHQPQPAGPTVGRVLGRPMEDVRATYIFGRELG 87
Query: 181 RGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
RGQFGVTYLVTHK TK+QFACKSIAT EVQIMHHL
Sbjct: 88 RGQFGVTYLVTHKETKQQFACKSIATRKLINKDDVEDVRREVQIMHHL 135
>gb|KRH59993.1| hypothetical protein GLYMA_05G213200 [Glycine max]
Length = 426
Score = 122 bits (305), Expect = 1e-30
Identities = 69/116 (59%), Positives = 78/116 (67%), Gaps = 9/116 (7%)
Frame = +1
Query: 4 SEPSSHPKPENTTPPTH--ISVLPPDRNP-----KQP--TSTTSTMGRVLGRPMEDVRST 156
S + P + P H I+VLPP+ NP K P +S++S++GRVLGRPMEDVRS
Sbjct: 5 SSGAGAPTTYSDDHPHHNGITVLPPNSNPSPQLPKPPPTSSSSSSLGRVLGRPMEDVRSI 64
Query: 157 YIFGRELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
YIFGRELGRGQFGVTYLVTHK TKEQFACKSIAT EVQIMHHL
Sbjct: 65 YIFGRELGRGQFGVTYLVTHKATKEQFACKSIATRKLVNRDDIDDIRREVQIMHHL 120
>ref|XP_017442255.1| PREDICTED: calcium-dependent protein kinase 3-like [Vigna
angularis]
gb|KOM57160.1| hypothetical protein LR48_Vigan11g019200 [Vigna angularis]
dbj|BAT73021.1| hypothetical protein VIGAN_01047500 [Vigna angularis var.
angularis]
Length = 520
Score = 122 bits (307), Expect = 2e-30
Identities = 63/93 (67%), Positives = 68/93 (73%), Gaps = 3/93 (3%)
Frame = +1
Query: 55 ISVLPPDRNPKQPT---STTSTMGRVLGRPMEDVRSTYIFGRELGRGQFGVTYLVTHKVT 225
I+VLPP+ NP P +S++GRVLGRPMEDVRS YIFGRELGRGQFGVTYLVTHK T
Sbjct: 30 ITVLPPNTNPSPPPPKPQASSSLGRVLGRPMEDVRSIYIFGRELGRGQFGVTYLVTHKTT 89
Query: 226 KEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
KEQFACKSIAT EVQIMHHL
Sbjct: 90 KEQFACKSIATRKLVNRDDIDDIRREVQIMHHL 122
>ref|XP_016202154.1| calcium-dependent protein kinase 1 [Arachis ipaensis]
Length = 521
Score = 122 bits (307), Expect = 2e-30
Identities = 67/116 (57%), Positives = 76/116 (65%), Gaps = 8/116 (6%)
Frame = +1
Query: 1 NSEPSSHPK---PENTTPPTHISVLPPDRNPKQ-----PTSTTSTMGRVLGRPMEDVRST 156
N PSS E P I+VL P NP P+S+++T+GRVLGRPMEDVRS+
Sbjct: 11 NGAPSSQAHNNTAEANRPSNGINVLHPHSNPTPHKPPPPSSSSATIGRVLGRPMEDVRSS 70
Query: 157 YIFGRELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
Y+FGRELGRGQFGVTYLVTHK T+EQFACKSIAT EVQIMHHL
Sbjct: 71 YVFGRELGRGQFGVTYLVTHKTTREQFACKSIATRKLVNRDDIEDIRREVQIMHHL 126
>gb|PNX89517.1| calcium-dependent protein kinase 3-like protein [Trifolium
pratense]
Length = 232
Score = 117 bits (293), Expect = 2e-30
Identities = 65/107 (60%), Positives = 73/107 (68%), Gaps = 2/107 (1%)
Frame = +1
Query: 10 PSSHPK--PENTTPPTHISVLPPDRNPKQPTSTTSTMGRVLGRPMEDVRSTYIFGRELGR 183
P + PK P+N+TP +P QP +T T+GR+LGRPMEDVRS +IFGRELGR
Sbjct: 23 PQNLPKILPQNSTP-----------SPPQPPLST-TIGRILGRPMEDVRSIFIFGRELGR 70
Query: 184 GQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
GQFGVTYLVTHKVTKEQFACKSIAT EVQIMHHL
Sbjct: 71 GQFGVTYLVTHKVTKEQFACKSIATRKLINRDDIDDVRREVQIMHHL 117
>ref|XP_020223591.1| calcium-dependent protein kinase 1 [Cajanus cajan]
Length = 509
Score = 122 bits (306), Expect = 2e-30
Identities = 64/106 (60%), Positives = 71/106 (66%), Gaps = 2/106 (1%)
Frame = +1
Query: 13 SSHPKPENTTPPTH--ISVLPPDRNPKQPTSTTSTMGRVLGRPMEDVRSTYIFGRELGRG 186
SS+ + P H I+VLPP+ P P S++GRVLGRPMEDVRS Y+FG ELGRG
Sbjct: 6 SSNSTYSHDPPHRHNGIAVLPPNSTPSPPPPAPSSLGRVLGRPMEDVRSMYVFGPELGRG 65
Query: 187 QFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
QFGVTYLVTHK TKEQFACKSIAT EVQIMHHL
Sbjct: 66 QFGVTYLVTHKATKEQFACKSIATRKLLNRDDIEDIRREVQIMHHL 111
>ref|XP_003525360.1| PREDICTED: calcium-dependent protein kinase 3-like [Glycine max]
gb|KRH59992.1| hypothetical protein GLYMA_05G213200 [Glycine max]
Length = 518
Score = 122 bits (305), Expect = 3e-30
Identities = 69/116 (59%), Positives = 78/116 (67%), Gaps = 9/116 (7%)
Frame = +1
Query: 4 SEPSSHPKPENTTPPTH--ISVLPPDRNP-----KQP--TSTTSTMGRVLGRPMEDVRST 156
S + P + P H I+VLPP+ NP K P +S++S++GRVLGRPMEDVRS
Sbjct: 5 SSGAGAPTTYSDDHPHHNGITVLPPNSNPSPQLPKPPPTSSSSSSLGRVLGRPMEDVRSI 64
Query: 157 YIFGRELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
YIFGRELGRGQFGVTYLVTHK TKEQFACKSIAT EVQIMHHL
Sbjct: 65 YIFGRELGRGQFGVTYLVTHKATKEQFACKSIATRKLVNRDDIDDIRREVQIMHHL 120
>ref|XP_013447139.1| calmodulin-domain kinase CDPK protein [Medicago truncatula]
gb|KEH21166.1| calmodulin-domain kinase CDPK protein [Medicago truncatula]
Length = 516
Score = 120 bits (302), Expect = 8e-30
Identities = 66/107 (61%), Positives = 74/107 (69%), Gaps = 2/107 (1%)
Frame = +1
Query: 10 PSSHPKPENTTPPTHISVLPPD--RNPKQPTSTTSTMGRVLGRPMEDVRSTYIFGRELGR 183
P S P P+N PT +LP + +P QP T+T+GR+L RPMEDVRS +IFGRELGR
Sbjct: 20 PHSDPPPKNA--PT---ILPQNFTPSPPQPPLPTTTLGRILNRPMEDVRSIFIFGRELGR 74
Query: 184 GQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
GQFGVTYLVTHKVTKEQFACKSIAT EVQIMHHL
Sbjct: 75 GQFGVTYLVTHKVTKEQFACKSIATRKLVNRDDIDDVRREVQIMHHL 121
>ref|XP_024173288.1| calcium-dependent protein kinase 1 [Rosa chinensis]
gb|PRQ17539.1| putative protein kinase CAMK-CDPK family [Rosa chinensis]
Length = 516
Score = 120 bits (301), Expect = 1e-29
Identities = 65/110 (59%), Positives = 71/110 (64%), Gaps = 9/110 (8%)
Frame = +1
Query: 22 PKPENTTPPTHISVLPPDRNPKQPTS---------TTSTMGRVLGRPMEDVRSTYIFGRE 174
P+P N H+ V PP+ P +P TTST GRVLG+PMEDVRSTY FGRE
Sbjct: 15 PRPSNG----HVRVRPPNAAPTRPAPPPKPHTTAPTTSTTGRVLGKPMEDVRSTYTFGRE 70
Query: 175 LGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
LGRGQFGVTYLVTHK TK+QFACKSIAT EVQIMHHL
Sbjct: 71 LGRGQFGVTYLVTHKETKQQFACKSIATRKLINHDDVEDVRREVQIMHHL 120
>ref|XP_021838451.1| calcium-dependent protein kinase 1 [Spinacia oleracea]
Length = 532
Score = 120 bits (301), Expect = 1e-29
Identities = 65/115 (56%), Positives = 72/115 (62%), Gaps = 7/115 (6%)
Frame = +1
Query: 1 NSEPSSHPKPENTTPPTHISVLPPDRNPKQ-------PTSTTSTMGRVLGRPMEDVRSTY 159
N P P+P + TPP PP + +Q P S +S GRVLGRPMEDVR+TY
Sbjct: 27 NPPPPQPPQPHHDTPPP-----PPQQQQQQQHQQKQPPQSLSSNFGRVLGRPMEDVRATY 81
Query: 160 IFGRELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
IFGRELGRGQFG+TYLVTHK TK QFACKSIAT EVQIMHHL
Sbjct: 82 IFGRELGRGQFGITYLVTHKKTKLQFACKSIATRKLLNRDDVEDVRREVQIMHHL 136
>ref|XP_015388666.1| PREDICTED: calcium-dependent protein kinase 3-like [Citrus
sinensis]
Length = 201
Score = 114 bits (285), Expect = 1e-29
Identities = 58/105 (55%), Positives = 70/105 (66%)
Frame = +1
Query: 10 PSSHPKPENTTPPTHISVLPPDRNPKQPTSTTSTMGRVLGRPMEDVRSTYIFGRELGRGQ 189
P HP PP PP + ++T++++GRVLG+PMEDVR+TYIFGRELGRGQ
Sbjct: 25 PGHHPNGLKIDPPG-----PPGPSSSATSATSASVGRVLGKPMEDVRNTYIFGRELGRGQ 79
Query: 190 FGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
FGVTYLVTHK TK+QFACKSI++ EVQIMHHL
Sbjct: 80 FGVTYLVTHKDTKQQFACKSISSRKLINRDDVEDVRREVQIMHHL 124
>ref|XP_024028142.1| calcium-dependent protein kinase 1 [Morus notabilis]
ref|XP_024028143.1| calcium-dependent protein kinase 1 [Morus notabilis]
Length = 526
Score = 120 bits (300), Expect = 2e-29
Identities = 64/118 (54%), Positives = 73/118 (61%), Gaps = 13/118 (11%)
Frame = +1
Query: 10 PSSHPKPENTTPPTHISVLPPDRNPKQPT-------------STTSTMGRVLGRPMEDVR 150
P H +P N ++VLPPD P P ++ + +GRVLGRPMEDVR
Sbjct: 17 PPHHHRPSNDA----VNVLPPDSRPSPPPPRPPHFPAAAATPTSAAAVGRVLGRPMEDVR 72
Query: 151 STYIFGRELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
STYIFGRELGRGQFGVTYLVTHK TK+QFACKSIAT EVQIMHH+
Sbjct: 73 STYIFGRELGRGQFGVTYLVTHKETKQQFACKSIATRKLVNRDDVEDVRREVQIMHHM 130
>gb|OAY59691.1| hypothetical protein MANES_01G050900, partial [Manihot esculenta]
Length = 426
Score = 119 bits (297), Expect = 2e-29
Identities = 67/116 (57%), Positives = 76/116 (65%), Gaps = 8/116 (6%)
Frame = +1
Query: 1 NSEPSSHPKPENTT---PPTHISVL-PPDRNPKQPTS----TTSTMGRVLGRPMEDVRST 156
+S SSHP N T PP+ S PP + QP++ TT +GRVLGRPMEDVR+T
Sbjct: 18 SSAASSHPPHSNVTTLHPPSASSTTRPPPPHSLQPSNRPSPTTPPVGRVLGRPMEDVRNT 77
Query: 157 YIFGRELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
Y FGRELGRGQFGVTYLVTHK TK+QFACKSI+T EVQIMHHL
Sbjct: 78 YTFGRELGRGQFGVTYLVTHKETKQQFACKSISTRRLIKSDDIEDVRREVQIMHHL 133
>gb|KNA21138.1| hypothetical protein SOVF_046030 [Spinacia oleracea]
Length = 608
Score = 120 bits (301), Expect = 2e-29
Identities = 65/115 (56%), Positives = 72/115 (62%), Gaps = 7/115 (6%)
Frame = +1
Query: 1 NSEPSSHPKPENTTPPTHISVLPPDRNPKQ-------PTSTTSTMGRVLGRPMEDVRSTY 159
N P P+P + TPP PP + +Q P S +S GRVLGRPMEDVR+TY
Sbjct: 103 NPPPPQPPQPHHDTPPP-----PPQQQQQQQHQQKQPPQSLSSNFGRVLGRPMEDVRATY 157
Query: 160 IFGRELGRGQFGVTYLVTHKVTKEQFACKSIATXXXXXXXXXXXXXXEVQIMHHL 324
IFGRELGRGQFG+TYLVTHK TK QFACKSIAT EVQIMHHL
Sbjct: 158 IFGRELGRGQFGITYLVTHKKTKLQFACKSIATRKLLNRDDVEDVRREVQIMHHL 212