BLASTX nr result
ID: Astragalus24_contig00006450
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Astragalus24_contig00006450
(1572 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase;... 667 0.0
dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata] 665 0.0
ref|NP_001296643.1| isoflavone 2'-hydroxylase-like [Cicer arieti... 661 0.0
ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum] ... 652 0.0
ref|XP_004504638.1| PREDICTED: isoflavone 2'-hydroxylase-like [C... 651 0.0
ref|XP_004504637.1| PREDICTED: isoflavone 2'-hydroxylase-like [C... 650 0.0
ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago ... 647 0.0
dbj|BAT21113.1| cytochrome P450 [synthetic construct] 638 0.0
dbj|BAA93634.1| cytochrome P450 [Lotus japonicus] 618 0.0
gb|AHG97656.1| isoflavone-2'-hydroxylase [Astragalus mongholicus] 605 0.0
dbj|BAT21114.1| cytochrome P450 [synthetic construct] 595 0.0
ref|XP_017405614.1| PREDICTED: isoflavone 2'-hydroxylase-like [V... 589 0.0
gb|KOM25514.1| hypothetical protein LR48_Vigan107s003300 [Vigna ... 589 0.0
ref|XP_015957959.1| isoflavone 2'-hydroxylase [Arachis duranensis] 588 0.0
ref|XP_016191270.1| isoflavone 2'-hydroxylase [Arachis ipaensis] 586 0.0
ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [G... 583 0.0
ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phas... 580 0.0
ref|XP_014506446.2| isoflavone 2'-hydroxylase [Vigna radiata var... 579 0.0
ref|XP_014498975.1| isoflavone 2'-hydroxylase [Vigna radiata var... 529 0.0
dbj|BAT80225.1| hypothetical protein VIGAN_02322300 [Vigna angul... 522 e-179
>sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase; AltName: Full=CYP GE-3;
AltName: Full=Cytochrome P450 81E1; AltName:
Full=Cytochrome P450 91A4
dbj|BAA22422.1| cytochrome P450 [Glycyrrhiza echinata]
Length = 499
Score = 667 bits (1720), Expect = 0.0
Identities = 333/421 (79%), Positives = 365/421 (86%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
M ILS LSYS F LALFFIF ++ ++RK K LHHLKRPLHRTF LS
Sbjct: 1 MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLS 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
EKYGHVFSLWFGSRLVVVVSS SEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGS SY
Sbjct: 61 EKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
GE WRNLRRITALDVLSN RINSFSGIR+DETQRLIT+LA+DSST+F E+ELSSR YDMT
Sbjct: 121 GEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMISGKRYYGED D +LQEAS+FRDM++E+LQL+G NNKTDF+PLL FLDFENL
Sbjct: 181 FNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAML 1208
E +LK IS K DAFLRGL++E R KKER NTMIDHLL+LQ+SQPEYYTDQIIKGLALAML
Sbjct: 241 EKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAML 300
Query: 1209 LAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINET 1388
LAGTDSSAVTL+WS+S+LLN+PEVLKKV+DELDTHVG DRLV+ESDLPKLTYLKNVINET
Sbjct: 301 LAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINET 360
Query: 1389 XXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKE 1568
HSTSDEC IGGYKVP+DTIV++NAWAI RDPELW+EATTFKPERFEK+
Sbjct: 361 LRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKK 420
Query: 1569 G 1571
G
Sbjct: 421 G 421
>dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]
Length = 499
Score = 665 bits (1716), Expect = 0.0
Identities = 332/421 (78%), Positives = 365/421 (86%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
M ILS LSYS F LALFFIF ++ ++RK K LHHLKRPLHRTF LS
Sbjct: 1 MDILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLS 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
EKYGHV SLWFGSRLVVVVSS SEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGS SY
Sbjct: 61 EKYGHVISLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
GE WRNLRRITALDVLSN RINSFSGIR+DETQRLIT+LA+DSST+F E+ELS RFYDMT
Sbjct: 121 GEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEIELSYRFYDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMISGKRYYGED D+ +LQEAS+FRDM++E+LQL+G NNKTDF+PLL FLDFENL
Sbjct: 181 FNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAML 1208
E +LK IS K DAFLRGL+QE R KKER NTMIDHLL+LQ+SQPEYYTDQIIKGLALAML
Sbjct: 241 EKRLKDISGKTDAFLRGLIQEHRAKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAML 300
Query: 1209 LAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINET 1388
LAGTDSSAVTL+WS+S+LLN+PEVLKKV+DELDTHVG DRLV+ESDLPKL+YLKNVINET
Sbjct: 301 LAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLSYLKNVINET 360
Query: 1389 XXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKE 1568
HSTSDEC IGGYKVP+DTIV++NAWAI RDPELW+EATTFKPERFEK+
Sbjct: 361 LRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKK 420
Query: 1569 G 1571
G
Sbjct: 421 G 421
>ref|NP_001296643.1| isoflavone 2'-hydroxylase-like [Cicer arietinum]
emb|CAB41490.1| cytochrome P450 monooxygenase [Cicer arietinum]
Length = 498
Score = 661 bits (1706), Expect = 0.0
Identities = 328/421 (77%), Positives = 363/421 (86%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
MGI SF +YS F L++FFIF+L+FQSRK K HHLKRPLHRTF LS
Sbjct: 1 MGIFSFFAYSLFYLSIFFIFRLLFQSRKFKNLPPGPPSLPIIGNFHHLKRPLHRTFKGLS 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
+ YG + SLWFGSRLVVVVSS SEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGS SY
Sbjct: 61 KTYGDIISLWFGSRLVVVVSSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
E WRNLRRIT+LDVLSN RIN+FSG+R+DETQRLITKLAEDSST F EVELS RFYDMT
Sbjct: 121 SEHWRNLRRITSLDVLSNHRINNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMISGKRYYGED D+ +LQEAS+FRDM+ E+LQL+G NNKTDF+PLL F DFENL
Sbjct: 181 FNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVTELLQLSGANNKTDFMPLLKFFDFENL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAML 1208
E ++K I++K DAFLRGLLQEQR+KKERTNTMIDHLL+LQESQPEYYTDQIIKGLALAML
Sbjct: 241 EKRVKNIADKTDAFLRGLLQEQRNKKERTNTMIDHLLNLQESQPEYYTDQIIKGLALAML 300
Query: 1209 LAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINET 1388
LAGTDSSAVTL+WS+S+LLN+PEVLKKVRDELDTHVG DRLV+ESDLPKLTYLKNVI ET
Sbjct: 301 LAGTDSSAVTLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPKLTYLKNVIYET 360
Query: 1389 XXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKE 1568
HSTSD+CIIGGYKVPRDTIV++NAWAI RDP+ WSEATTFKPERF+K+
Sbjct: 361 LRLCTPAPLLLPHSTSDDCIIGGYKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKK 420
Query: 1569 G 1571
G
Sbjct: 421 G 421
>ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum]
emb|CAA10067.1| cytochrome P450 [Cicer arietinum]
Length = 498
Score = 652 bits (1683), Expect = 0.0
Identities = 324/421 (76%), Positives = 360/421 (85%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
MGILS L YS F L+ FFI +L+FQSRK K LHHLKRPLHRTF LS
Sbjct: 1 MGILSLLCYSLFYLSFFFIIRLLFQSRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLS 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
+ YG + SLWFGSRLVVVVSS SEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGS SY
Sbjct: 61 KTYGDIISLWFGSRLVVVVSSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
E WRNLRRIT+LDVLSN RIN+FSG+R+DETQRLITKLAEDSST F EVELS RFYDMT
Sbjct: 121 SEHWRNLRRITSLDVLSNHRINNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMI GKRYYGED D+ +LQEAS+FRDM+ E+LQL+G NNKTDF+PLL F DFENL
Sbjct: 181 FNNIMRMIPGKRYYGEDCDMSDLQEASQFRDMVTELLQLSGANNKTDFMPLLKFFDFENL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAML 1208
E ++K I++K DAFLRGLLQEQR+KKERTNTMIDHLL+LQESQPEYYTDQIIKGLALAML
Sbjct: 241 EKRVKNIADKTDAFLRGLLQEQRNKKERTNTMIDHLLNLQESQPEYYTDQIIKGLALAML 300
Query: 1209 LAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINET 1388
LAGTDSSAVTL+WS+S+LLN+PE+LKKV+DELD HVG DRLV+ESDLPKLTYLKNVI ET
Sbjct: 301 LAGTDSSAVTLEWSMSNLLNHPEILKKVKDELDIHVGQDRLVDESDLPKLTYLKNVIYET 360
Query: 1389 XXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKE 1568
HSTSD+CIIGGYKVPRDTIV++NAWAI RDP+ WSEAT+FKPERF+K+
Sbjct: 361 LRLCTPAPLLLPHSTSDDCIIGGYKVPRDTIVLINAWAIHRDPKSWSEATSFKPERFDKK 420
Query: 1569 G 1571
G
Sbjct: 421 G 421
>ref|XP_004504638.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
Length = 498
Score = 651 bits (1679), Expect = 0.0
Identities = 322/421 (76%), Positives = 358/421 (85%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
MGI S L YS F L+LFF+ +L+FQSRK K LHHLKRPLHRTF LS
Sbjct: 1 MGIFSLLCYSLFYLSLFFVIRLLFQSRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLS 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
+ YG + SLWFGSRLVVVVSS SEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGS SY
Sbjct: 61 KTYGDIISLWFGSRLVVVVSSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
E WRNLRRIT+LDVLSN RIN+FSG+R+DETQRLITKLAEDSST F EVELS RFYDMT
Sbjct: 121 SEHWRNLRRITSLDVLSNHRINNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMISGKRYYGED D+ +LQEAS+FRDM+ E+LQL+G NNKTDF+PLL F DFENL
Sbjct: 181 FNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVTELLQLSGANNKTDFLPLLKFFDFENL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAML 1208
E ++K I+ K D FLRGLLQEQR+KKERTNTMIDHLL+LQESQPEYYTDQIIKGLALAML
Sbjct: 241 EKRVKNIANKTDTFLRGLLQEQRNKKERTNTMIDHLLNLQESQPEYYTDQIIKGLALAML 300
Query: 1209 LAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINET 1388
LAGTDSSAVTL+WS+ +L+N+PE+LKKV+DELD HVG DRLV+ESDLPKLTYLKNVI ET
Sbjct: 301 LAGTDSSAVTLEWSMCNLMNHPEILKKVKDELDIHVGQDRLVDESDLPKLTYLKNVIYET 360
Query: 1389 XXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKE 1568
HSTSD+CIIGGYKVPRDTIV++NAWAI RDP+ WSEATTFKPERF+K+
Sbjct: 361 LRLCTPAPLLLPHSTSDDCIIGGYKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKK 420
Query: 1569 G 1571
G
Sbjct: 421 G 421
>ref|XP_004504637.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
Length = 511
Score = 650 bits (1678), Expect = 0.0
Identities = 322/421 (76%), Positives = 359/421 (85%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
M I S L YS F L++FFI +L+FQSR+ K LHHLKRPLHRTF L+
Sbjct: 1 MEIFSLLCYSLFYLSIFFIIRLLFQSRRFKNLPPGPPSLPIIGNLHHLKRPLHRTFKELT 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
+KYG V SLWFGSRLVVVVSS SEFQQCFTKNDVVLANRP+F+SGKYIFYNYTTLGS SY
Sbjct: 61 KKYGDVISLWFGSRLVVVVSSLSEFQQCFTKNDVVLANRPKFISGKYIFYNYTTLGSASY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
GE WRNLRRIT+LDVLSN RIN+FSGIRKDETQRLI KLAEDSS++F EVELS RFYDMT
Sbjct: 121 GEHWRNLRRITSLDVLSNHRINNFSGIRKDETQRLIKKLAEDSSSNFAEVELSFRFYDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMISGKRYYGED D+ +LQEAS+FRDM+ E+LQL+G NNKTDF+PLL FLDFENL
Sbjct: 181 FNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVTELLQLSGANNKTDFLPLLRFLDFENL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAML 1208
E K+K I EK DAFL GLLQEQR+KKERTNTMIDHLLS+QESQPEYYTDQIIKGL LAML
Sbjct: 241 EKKVKNIGEKTDAFLSGLLQEQRNKKERTNTMIDHLLSMQESQPEYYTDQIIKGLVLAML 300
Query: 1209 LAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINET 1388
LAGTD++A+TL+WS+S+LLN+PEVLKKVRDELDTHVG DRLV+ESDLP LTY+KNVI ET
Sbjct: 301 LAGTDTTALTLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIKNVIYET 360
Query: 1389 XXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKE 1568
HSTSD+CIIGGYK+PRDTIV++NAW IQRDPE WSEATTFKPERF K+
Sbjct: 361 LRLYGPAPLLIPHSTSDDCIIGGYKIPRDTIVLINAWGIQRDPESWSEATTFKPERFNKK 420
Query: 1569 G 1571
G
Sbjct: 421 G 421
>ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago truncatula]
sp|Q6WNR0.1|C81E7_MEDTR RecName: Full=Isoflavone 2'-hydroxylase; AltName:
Full=4'-methoxyisoflavone 2'-hydroxylase; AltName:
Full=Cytochrome P450 81E7
gb|AAQ20040.1| isoflavone 2'-hydroxylase [Medicago truncatula]
gb|KEH31433.1| cytochrome P450 family 81 protein [Medicago truncatula]
Length = 498
Score = 647 bits (1669), Expect = 0.0
Identities = 319/421 (75%), Positives = 362/421 (85%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
MGILS+L YS F L++FFI +L+FQSRK K LHHLKRPL+RTF L+
Sbjct: 1 MGILSYLCYSLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALT 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
EKYG+V SLWFGSRLVVVVSS SEFQ+CFTKNDVVLANRPRFLSGKYIFYNYTTLGS SY
Sbjct: 61 EKYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
GE WRNLRRIT+LDVLSN RIN+F+ IR+DETQRLI KLAEDSST F EVEL+ RF+DMT
Sbjct: 121 GEHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMISGKRYYG+D D+ E+QEAS+FRDM++E+LQL+G NNKTDF+PLL FLDFENL
Sbjct: 181 FNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAML 1208
E ++K+I EKND FL GLLQEQR KKERTNTMIDHLL++QESQPEYYTD IIKGL LAML
Sbjct: 241 EKRVKRIGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAML 300
Query: 1209 LAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINET 1388
LAGTDSSAVTL+W++S++LN PEVLKKVRDE+DTHVG DRLV+ESDLPKLTYL+NVI ET
Sbjct: 301 LAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYET 360
Query: 1389 XXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKE 1568
HST+DECI+GGYKVPRDTIV++NAWAI RDPE WSEATTFKPERF+K+
Sbjct: 361 LRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKK 420
Query: 1569 G 1571
G
Sbjct: 421 G 421
>dbj|BAT21113.1| cytochrome P450 [synthetic construct]
Length = 477
Score = 638 bits (1645), Expect = 0.0
Identities = 317/399 (79%), Positives = 348/399 (87%)
Frame = +3
Query: 375 IFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLSEKYGHVFSLWFGSRLVVVVSSP 554
+ ++RK K LHHLKRPLHRTF LSEKYGHVFSLWFGSRLVVVVSS
Sbjct: 1 MIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSA 60
Query: 555 SEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRIN 734
SEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGS SYGE WRNLRRITALDVLSN RIN
Sbjct: 61 SEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRIN 120
Query: 735 SFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVLE 914
SFSGIR+DETQRLIT+LA+DSST+F E+ELSSR YDMTFNNIMRMISGKRYYGED D +
Sbjct: 121 SFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSD 180
Query: 915 LQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQEQ 1094
LQEAS+FRDM++E+LQL+G NNKTDF+PLL FLDFENLE +LK IS K DAFLRGL++E
Sbjct: 181 LQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEH 240
Query: 1095 RDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNNP 1274
R KKER NTMIDHLL+LQ+SQPEYYTDQIIKGLALAMLLAGTDSSAVTL+WS+S+LLN+P
Sbjct: 241 RTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHP 300
Query: 1275 EVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECIIG 1454
EVLKKV+DELDTHVG DRLV+ESDLPKLTYLKNVINET HSTSDEC IG
Sbjct: 301 EVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIG 360
Query: 1455 GYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1571
GYKVP+DTIV++NAWAI RDPELW+EATTFKPERFEK+G
Sbjct: 361 GYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKG 399
>dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]
Length = 499
Score = 618 bits (1594), Expect = 0.0
Identities = 310/422 (73%), Positives = 350/422 (82%), Gaps = 1/422 (0%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
M I+SFL YS F +ALF I KL SRK K LHHLKRPLHRTF LS
Sbjct: 1 MDIISFLYYSLFYVALFAIIKLFLGSRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALS 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
EKYG VFSLWFG+RLVVVVSS ++ Q+CFTKNDVVLANRPRFLSGKYIFYNYTTLGS SY
Sbjct: 61 EKYGDVFSLWFGNRLVVVVSSFADVQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
GE WRNLRRIT+LDVLSN RINSFS IR+DET RLI KLAEDS+ +F EVEL+SRF+DMT
Sbjct: 121 GEHWRNLRRITSLDVLSNHRINSFSPIRRDETTRLIRKLAEDSAKNFSEVELTSRFFDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMISGKRYYGED D+ ELQEAS FRDM+ E+LQL+G NNK DF+P+L +DFE L
Sbjct: 181 FNNIMRMISGKRYYGEDCDMTELQEASEFRDMVTELLQLSGANNKADFMPILRLVDFEGL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERT-NTMIDHLLSLQESQPEYYTDQIIKGLALAM 1205
E ++K IS K D FLRGLLQE RDKK+RT NTMIDHLL+LQESQPEYYTDQIIKGLALAM
Sbjct: 241 EKRVKGISSKTDRFLRGLLQEHRDKKQRTANTMIDHLLTLQESQPEYYTDQIIKGLALAM 300
Query: 1206 LLAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINE 1385
LLAGTDSSAVTL+WS+ ++LN PEVL+K++ ELDTHVG DRLV+ESD+PKLTYLKNVINE
Sbjct: 301 LLAGTDSSAVTLEWSMCNVLNYPEVLEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINE 360
Query: 1386 TXXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEK 1565
T HS SD+C IGGYKVPRDTIV++NAWA+ RDP+LW+EATTFKPERF+K
Sbjct: 361 TLRLYTPAPLLLPHSASDDCTIGGYKVPRDTIVLINAWALHRDPQLWTEATTFKPERFDK 420
Query: 1566 EG 1571
+G
Sbjct: 421 KG 422
>gb|AHG97656.1| isoflavone-2'-hydroxylase [Astragalus mongholicus]
Length = 498
Score = 605 bits (1561), Expect = 0.0
Identities = 295/421 (70%), Positives = 345/421 (81%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
M ILSFL YS F L LFFIF LIFQSRK K LHHLKRPL TF LS
Sbjct: 1 MEILSFLYYSLFYLTLFFIFNLIFQSRKFKNLPPGPPSLPIIGNLHHLKRPLQHTFKGLS 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
EKYG + SLWFGSRLVV+VSS + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS SY
Sbjct: 61 EKYGDIMSLWFGSRLVVIVSSQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
GE WRNLRRITA DVLS RINSF+GIR DE RL+ KLAEDSS DFVEVEL+S+FYDMT
Sbjct: 121 GEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQKLAEDSSEDFVEVELTSKFYDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMISGKRYYG+D D+ +++EA++FR M++++L+L+G NNK DF+P+L +D+ENL
Sbjct: 181 FNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAML 1208
E +L KI +KNDAFLRGL++E R KK+ NTM+DHLLSLQESQPEYYTDQIIKGL +L
Sbjct: 241 EKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGIL 300
Query: 1209 LAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINET 1388
LAGTDSSAVTL+W+LS +LNNPEVLKK RDELDTH+G DRL+EESDLPK+ YLKN++ ET
Sbjct: 301 LAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYET 360
Query: 1389 XXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKE 1568
HS+S+ECII GYKVPRDTI++ NAWAI RDP++WSEAT+FKPERF+KE
Sbjct: 361 LRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKE 420
Query: 1569 G 1571
G
Sbjct: 421 G 421
>dbj|BAT21114.1| cytochrome P450 [synthetic construct]
Length = 477
Score = 595 bits (1533), Expect = 0.0
Identities = 296/397 (74%), Positives = 334/397 (84%), Gaps = 1/397 (0%)
Frame = +3
Query: 384 SRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLSEKYGHVFSLWFGSRLVVVVSSPSEF 563
SRK K LHHLKRPLHRTF LSEKYG VFSLWFG+RLVVVVSS ++
Sbjct: 4 SRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSSFADV 63
Query: 564 QQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRINSFS 743
Q+CFTKNDVVLANRPRFLSGKYIFYNYTTLGS SYGE WRNLRRIT+LDVLSN RINSFS
Sbjct: 64 QECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRINSFS 123
Query: 744 GIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVLELQE 923
IR+DET RLI KLAEDS+ +F EVEL+SRF+DMTFNNIMRMISGKRYYGED D+ ELQE
Sbjct: 124 PIRRDETTRLIRKLAEDSAKNFSEVELTSRFFDMTFNNIMRMISGKRYYGEDCDMTELQE 183
Query: 924 ASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQEQRDK 1103
AS FRDM+ E+LQL+G NNK DF+P+L +DFE LE ++K IS K D FLRGLLQE RDK
Sbjct: 184 ASEFRDMVTELLQLSGANNKADFMPILRLVDFEGLEKRVKGISSKTDRFLRGLLQEHRDK 243
Query: 1104 KERT-NTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNNPEV 1280
K+RT NTMIDHLL+LQESQPEYYTDQIIKGLALAMLLAGTDSSAVTL+WS+ ++LN PEV
Sbjct: 244 KQRTANTMIDHLLTLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNVLNYPEV 303
Query: 1281 LKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECIIGGY 1460
L+K++ ELDTHVG DRLV+ESD+PKLTYLKNVINET HS SD+C IGGY
Sbjct: 304 LEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCTIGGY 363
Query: 1461 KVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1571
KVPRDTIV++NAWA+ RDP+LW+EATTFKPERF+K+G
Sbjct: 364 KVPRDTIVLINAWALHRDPQLWTEATTFKPERFDKKG 400
>ref|XP_017405614.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vigna angularis]
Length = 500
Score = 589 bits (1518), Expect = 0.0
Identities = 287/418 (68%), Positives = 340/418 (81%)
Frame = +3
Query: 318 LSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLSEKY 497
LS LSYSF L LF +F+L+ Q+RK + LHHLKRPLHRTF LS+ Y
Sbjct: 6 LSILSYSFLYLLLFLVFQLLLQARKFQNLPPGPPSLPIFGNLHHLKRPLHRTFKALSDNY 65
Query: 498 GHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSYGEL 677
GHV SLWFGSRLVVVVSS S FQ CFTKNDV LANRPRFLSGKYIFYNYTTLGS YG
Sbjct: 66 GHVISLWFGSRLVVVVSSHSLFQHCFTKNDVALANRPRFLSGKYIFYNYTTLGSSPYGHH 125
Query: 678 WRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNN 857
WRNLRRITAL++LS RIN+F+ IR+DET RLI KLAED+S+DF EVEL+S+FYDMTFNN
Sbjct: 126 WRNLRRITALELLSTHRINTFADIRRDETHRLIRKLAEDASSDFAEVELTSKFYDMTFNN 185
Query: 858 IMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETK 1037
IMRMISGKRYYG+D D+ +++EA +FR M++E+L+L+G NNK DF+PLL DFENLE +
Sbjct: 186 IMRMISGKRYYGDDCDMADVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDFENLEKR 245
Query: 1038 LKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAG 1217
LKKIS K D FL GLL+EQR +K+ NTM+DHLLSLQESQP+YYTDQIIKGLAL ML+AG
Sbjct: 246 LKKISSKTDTFLNGLLEEQRSRKQGANTMVDHLLSLQESQPDYYTDQIIKGLALGMLVAG 305
Query: 1218 TDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXX 1397
TDSSAVTL+W+LS +LN+PEVLK+ RDEL+ VG +RL+EESDLPKL YLKN+I ET
Sbjct: 306 TDSSAVTLEWALSCMLNHPEVLKRTRDELEKQVGQERLLEESDLPKLPYLKNIIFETLRL 365
Query: 1398 XXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1571
HS+S+EC+IGG+KVPRD+IV++NAW+I RDPE+WSEAT+FKPERFE EG
Sbjct: 366 YTPAPLLLPHSSSEECMIGGFKVPRDSIVLINAWSIHRDPEVWSEATSFKPERFESEG 423
>gb|KOM25514.1| hypothetical protein LR48_Vigan107s003300 [Vigna angularis]
dbj|BAT74198.1| hypothetical protein VIGAN_01181700 [Vigna angularis var. angularis]
Length = 504
Score = 589 bits (1518), Expect = 0.0
Identities = 287/418 (68%), Positives = 340/418 (81%)
Frame = +3
Query: 318 LSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLSEKY 497
LS LSYSF L LF +F+L+ Q+RK + LHHLKRPLHRTF LS+ Y
Sbjct: 10 LSILSYSFLYLLLFLVFQLLLQARKFQNLPPGPPSLPIFGNLHHLKRPLHRTFKALSDNY 69
Query: 498 GHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSYGEL 677
GHV SLWFGSRLVVVVSS S FQ CFTKNDV LANRPRFLSGKYIFYNYTTLGS YG
Sbjct: 70 GHVISLWFGSRLVVVVSSHSLFQHCFTKNDVALANRPRFLSGKYIFYNYTTLGSSPYGHH 129
Query: 678 WRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNN 857
WRNLRRITAL++LS RIN+F+ IR+DET RLI KLAED+S+DF EVEL+S+FYDMTFNN
Sbjct: 130 WRNLRRITALELLSTHRINTFADIRRDETHRLIRKLAEDASSDFAEVELTSKFYDMTFNN 189
Query: 858 IMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETK 1037
IMRMISGKRYYG+D D+ +++EA +FR M++E+L+L+G NNK DF+PLL DFENLE +
Sbjct: 190 IMRMISGKRYYGDDCDMADVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDFENLEKR 249
Query: 1038 LKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAG 1217
LKKIS K D FL GLL+EQR +K+ NTM+DHLLSLQESQP+YYTDQIIKGLAL ML+AG
Sbjct: 250 LKKISSKTDTFLNGLLEEQRSRKQGANTMVDHLLSLQESQPDYYTDQIIKGLALGMLVAG 309
Query: 1218 TDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXX 1397
TDSSAVTL+W+LS +LN+PEVLK+ RDEL+ VG +RL+EESDLPKL YLKN+I ET
Sbjct: 310 TDSSAVTLEWALSCMLNHPEVLKRTRDELEKQVGQERLLEESDLPKLPYLKNIIFETLRL 369
Query: 1398 XXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1571
HS+S+EC+IGG+KVPRD+IV++NAW+I RDPE+WSEAT+FKPERFE EG
Sbjct: 370 YTPAPLLLPHSSSEECMIGGFKVPRDSIVLINAWSIHRDPEVWSEATSFKPERFESEG 427
>ref|XP_015957959.1| isoflavone 2'-hydroxylase [Arachis duranensis]
Length = 499
Score = 588 bits (1516), Expect = 0.0
Identities = 287/421 (68%), Positives = 339/421 (80%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
M I++ L YS +A+FFIF L+ QSR K LHHLK+PLHR F LS
Sbjct: 1 MEIIAMLGYSVLSVAIFFIFTLLIQSRSFKNLPPGPPSLPIIGNLHHLKKPLHRHFRGLS 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
EKYG VFSLWFGSRLVVVVSSP+ Q+CFTKNDVVLANRPRFLSGKYIFYNYTT+GS Y
Sbjct: 61 EKYGDVFSLWFGSRLVVVVSSPTVVQECFTKNDVVLANRPRFLSGKYIFYNYTTVGSSEY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
GE WRNLRRIT+LDVLSN R+NSFS IR+DET RLI KL+EDS+ +F +VELSS+FYDMT
Sbjct: 121 GEHWRNLRRITSLDVLSNHRLNSFSPIRRDETTRLIRKLSEDSTENFAKVELSSKFYDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMISGKRYYGED D+ +++EA FR MI+EVLQL+G NNKTDFIPLLG LD +NL
Sbjct: 181 FNNIMRMISGKRYYGEDCDMADVEEAKHFRQMISEVLQLSGANNKTDFIPLLGMLDVDNL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAML 1208
+ +L IS + D FL GLLQE R K + NTMIDHLLSLQ+SQP+YYTDQIIKGL LAML
Sbjct: 241 KKRLVTISGRTDKFLNGLLQEHRTKNQHQNTMIDHLLSLQDSQPDYYTDQIIKGLGLAML 300
Query: 1209 LAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINET 1388
LAGTDSSAVTL+WS+ +LLN+PE L +VR+ELDTHVG DR+V+ESD+PK TYL+NVINET
Sbjct: 301 LAGTDSSAVTLEWSMCNLLNHPEALNRVREELDTHVGQDRIVDESDIPKFTYLRNVINET 360
Query: 1389 XXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKE 1568
HS++++C IGGYK+P+DTIV++N WAI RDP+ W EAT+FKPERFE++
Sbjct: 361 LRLYTPAPLLLPHSSAEKCTIGGYKIPKDTIVLINGWAIHRDPKNWDEATSFKPERFERK 420
Query: 1569 G 1571
G
Sbjct: 421 G 421
>ref|XP_016191270.1| isoflavone 2'-hydroxylase [Arachis ipaensis]
Length = 499
Score = 586 bits (1511), Expect = 0.0
Identities = 286/421 (67%), Positives = 339/421 (80%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
M I++ L YS +A+FFIF L+ QSR K LHHLK+PLHR F LS
Sbjct: 1 MEIIAMLGYSVLYVAIFFIFTLLIQSRSFKNLPPGPPSLPIIGNLHHLKKPLHRHFRGLS 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
EKYG VFSLWFGSRLVVVVSSP+ Q+CFTKNDVVLANRPRFLSGKYIFYNYTT+GS Y
Sbjct: 61 EKYGDVFSLWFGSRLVVVVSSPTVVQECFTKNDVVLANRPRFLSGKYIFYNYTTVGSSEY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMT 848
GE WRNLRRIT+LDVLSN R+NSFS IR+DET RLI KL+EDS+ +F +VELSS+FYDMT
Sbjct: 121 GEHWRNLRRITSLDVLSNHRLNSFSPIRRDETTRLIRKLSEDSTENFAKVELSSKFYDMT 180
Query: 849 FNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENL 1028
FNNIMRMISGKRYYGED D+ +++EA +FR MI+EVLQL+G NNKTDFIPLLG LD +NL
Sbjct: 181 FNNIMRMISGKRYYGEDCDMADVEEAKQFRQMISEVLQLSGANNKTDFIPLLGMLDVDNL 240
Query: 1029 ETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAML 1208
+ +L IS + D FL GLLQE R K + NTMIDHLLSLQ+SQP+YYTDQIIKGL LAML
Sbjct: 241 KKRLVTISGRTDKFLNGLLQEHRTKNQHQNTMIDHLLSLQDSQPDYYTDQIIKGLGLAML 300
Query: 1209 LAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINET 1388
LAGTDSSAVTL+WS+ +LLN+PE L +VR+ELDTHVG DR+V+ESD+PK TYL+NVINET
Sbjct: 301 LAGTDSSAVTLEWSMCNLLNHPEALNRVREELDTHVGQDRIVDESDIPKFTYLRNVINET 360
Query: 1389 XXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKE 1568
HS++++C IGGY +P+DTIV++N WAI RDP+ W EAT+FKPERFE++
Sbjct: 361 LRLYTPAPLLLPHSSAEKCTIGGYNIPKDTIVLINGWAIHRDPKNWDEATSFKPERFERK 420
Query: 1569 G 1571
G
Sbjct: 421 G 421
>ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
gb|KRH42431.1| hypothetical protein GLYMA_08G089500 [Glycine max]
Length = 502
Score = 583 bits (1503), Expect = 0.0
Identities = 289/425 (68%), Positives = 340/425 (80%), Gaps = 4/425 (0%)
Frame = +3
Query: 309 MGILSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLS 488
M ILSFLSYS + LF + KL+ Q+RK + LHHLKRPLHRTF LS
Sbjct: 1 MEILSFLSYSLLYVFLFLLLKLLLQARKFQNLPPGPPSLPIIGNLHHLKRPLHRTFRALS 60
Query: 489 EKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSY 668
+KYGHV SLWFGSRLVVVVSS + FQ+CFTKNDVVLANRPRFLSGK+IFYNYTTLGS Y
Sbjct: 61 DKYGHVISLWFGSRLVVVVSSQTLFQECFTKNDVVLANRPRFLSGKHIFYNYTTLGSSPY 120
Query: 669 GELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAE----DSSTDFVEVELSSRF 836
GE WRNLRRITALDVLS R++SF+ IR+DET RL+ KLAE +SS F EVEL+S+F
Sbjct: 121 GEHWRNLRRITALDVLSTHRLHSFAAIRRDETHRLVRKLAEAQGSESSLSFAEVELTSKF 180
Query: 837 YDMTFNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLD 1016
YDMTFNNIMRMISGKRYYG+D D+ +++EA +FR M++E+L+L G NNK DF+P+L D
Sbjct: 181 YDMTFNNIMRMISGKRYYGDDCDMADVEEAKQFRAMVSELLKLAGANNKNDFMPVLRLFD 240
Query: 1017 FENLETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLA 1196
FENLE +LKKIS K D FLRGLL+E R KK+R NTM+DHLLSLQESQPEYYTDQIIKGLA
Sbjct: 241 FENLEKRLKKISNKTDTFLRGLLEEIRAKKQRANTMLDHLLSLQESQPEYYTDQIIKGLA 300
Query: 1197 LAMLLAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNV 1376
L ML+A TDS AVTL+W+LS +LN+PEV K+ RDEL+THVG D L+EESDL KL YLKN+
Sbjct: 301 LGMLIAATDSQAVTLEWALSCVLNHPEVFKRARDELETHVGQDHLLEESDLSKLPYLKNI 360
Query: 1377 INETXXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPER 1556
I ET HS+S+ECIIGG+KVP DTIV++NAW+I RDP++WSEAT+FKPER
Sbjct: 361 IYETLRLYTPAPLLLPHSSSEECIIGGFKVPGDTIVLINAWSIHRDPKVWSEATSFKPER 420
Query: 1557 FEKEG 1571
FEKEG
Sbjct: 421 FEKEG 425
>ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
gb|ESW30851.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
Length = 500
Score = 580 bits (1496), Expect = 0.0
Identities = 282/418 (67%), Positives = 337/418 (80%)
Frame = +3
Query: 318 LSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLSEKY 497
LS LSYS + LF +F+L+ Q+R + LHHLKRPLHR F LS+ Y
Sbjct: 6 LSILSYSLLYVILFLLFQLLLQARNFQNLPPGPPSLPIIGNLHHLKRPLHRHFKALSDNY 65
Query: 498 GHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSYGEL 677
GHV SLWFGSRLVVVVSS S FQ CFTKND+ LANRPRFLSGKYIFYNYTTLGS YG
Sbjct: 66 GHVISLWFGSRLVVVVSSHSLFQHCFTKNDIALANRPRFLSGKYIFYNYTTLGSSPYGHH 125
Query: 678 WRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNN 857
WRNLRRITAL++LS QRIN+F+ IR+DET RLI KLAE +S+DF EVEL+S+FYDMTFNN
Sbjct: 126 WRNLRRITALELLSTQRINTFAAIRRDETHRLIRKLAEGASSDFAEVELTSKFYDMTFNN 185
Query: 858 IMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETK 1037
IMRMISGKRYYG+D D+ +++EA +FR M++E+L+L+G NNK DF+PLL DFENLE +
Sbjct: 186 IMRMISGKRYYGDDCDMADVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDFENLEKR 245
Query: 1038 LKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAG 1217
LKKIS K D FL GLL+E R KK+R NTM+DHLLSLQESQPEYYTDQIIKGLAL ML+AG
Sbjct: 246 LKKISSKTDTFLNGLLEEHRSKKQRANTMVDHLLSLQESQPEYYTDQIIKGLALGMLIAG 305
Query: 1218 TDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXX 1397
TDSSAVTL+W+LS ++N+PEV+K+ RDEL+THVG +RL++ESDL KL YLKN+I ET
Sbjct: 306 TDSSAVTLEWALSCVVNHPEVMKRARDELETHVGEERLLDESDLSKLPYLKNIIFETLRL 365
Query: 1398 XXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1571
HS+S+EC IGG+KVPRD+IV++NAW+I RDPE+WSEAT+FKPERFE EG
Sbjct: 366 YTPAPLLLPHSSSEECNIGGFKVPRDSIVLINAWSIHRDPEVWSEATSFKPERFENEG 423
>ref|XP_014506446.2| isoflavone 2'-hydroxylase [Vigna radiata var. radiata]
Length = 504
Score = 579 bits (1492), Expect = 0.0
Identities = 281/418 (67%), Positives = 338/418 (80%)
Frame = +3
Query: 318 LSFLSYSFFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLSEKY 497
LS LSYS L LF + +L+ Q+RK + LHHLKRPLHRTF LS+ Y
Sbjct: 10 LSILSYSLLYLLLFLLSQLLLQARKFQNLPPGPPSLPVIGNLHHLKRPLHRTFKALSDNY 69
Query: 498 GHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSYGEL 677
GHV SLWFGSRLVVVVSS S FQ CFTKND+ LANRPRFLSGKYIFYNYTTLGS YG
Sbjct: 70 GHVISLWFGSRLVVVVSSHSLFQHCFTKNDIALANRPRFLSGKYIFYNYTTLGSSPYGHH 129
Query: 678 WRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNN 857
WRNLRRITAL++LS RIN+F+ IR+DET RLI KLAED+S+DF EVEL+S+FYDMTFNN
Sbjct: 130 WRNLRRITALELLSTHRINTFADIRRDETHRLIRKLAEDASSDFAEVELTSKFYDMTFNN 189
Query: 858 IMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETK 1037
IMRMISGKRYYG+D D+ +++EA +FR M++E+L+L+G NNK DF+PLL D+ENLE +
Sbjct: 190 IMRMISGKRYYGDDCDMADVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDYENLEKR 249
Query: 1038 LKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAG 1217
LKKIS K D FL GLL+EQR KK+R NTM+DHLLSLQESQP+YYTD+IIKGLAL ML+AG
Sbjct: 250 LKKISSKTDTFLNGLLEEQRSKKQRANTMVDHLLSLQESQPDYYTDEIIKGLALGMLVAG 309
Query: 1218 TDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXX 1397
TDSSAVTL+W+LS +L +PEVLK+ RDEL+T VG +RL+EESD+ KL YLKN+I ET
Sbjct: 310 TDSSAVTLEWALSCVLKHPEVLKRTRDELETQVGQERLLEESDISKLPYLKNIIFETLRL 369
Query: 1398 XXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1571
HS+S+ECIIGG+KVPRD+IV++NAW+I RDP++WSEAT+FKPER+E EG
Sbjct: 370 YTPAPLLLPHSSSEECIIGGFKVPRDSIVLINAWSIHRDPQVWSEATSFKPERYESEG 427
>ref|XP_014498975.1| isoflavone 2'-hydroxylase [Vigna radiata var. radiata]
Length = 499
Score = 529 bits (1363), Expect = 0.0
Identities = 265/422 (62%), Positives = 326/422 (77%), Gaps = 1/422 (0%)
Frame = +3
Query: 309 MGILSFLSYS-FFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRL 485
M L L+YS FF L+L IFKL FQ+ K L+ L+RPLHR +R+
Sbjct: 1 METLPLLTYSLFFLLSLLIIFKLFFQTNH-KNLPPGPPPLPIIGNLNLLERPLHRFLHRM 59
Query: 486 SEKYGHVFSLWFGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVS 665
S+ +GHVFSLWFGSRL V+VSSPS FQ+CFTKNDV LANRPR LSGK+IFYNYTT+GS S
Sbjct: 60 SQTHGHVFSLWFGSRLAVIVSSPSAFQECFTKNDVALANRPRSLSGKHIFYNYTTVGSCS 119
Query: 666 YGELWRNLRRITALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDM 845
YGELWRNLRRIT+LDVLS QRI+SF+GIRKDET R+I LA+ S ++ VE+SS F+DM
Sbjct: 120 YGELWRNLRRITSLDVLSTQRIHSFAGIRKDETDRVIHSLAKASRMEYAHVEMSSMFHDM 179
Query: 846 TFNNIMRMISGKRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFEN 1025
T+N++MRM+SGKRYYG+++ +L +A FR+ + E+LQL G +NK D++P L + DF+N
Sbjct: 180 TYNSMMRMLSGKRYYGKEIQAKDLVKAKEFRETVEELLQLAGVSNKADYLPFLRWFDFQN 239
Query: 1026 LETKLKKISEKNDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAM 1205
LE KLK I+++ D FL L+ EQR+KKER NTMIDHLLSLQESQP+YY+D IIKGL LAM
Sbjct: 240 LEKKLKSINKRFDTFLDELIHEQRNKKERENTMIDHLLSLQESQPQYYSDHIIKGLVLAM 299
Query: 1206 LLAGTDSSAVTLDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINE 1385
L AGTDSSAVTL+WSLS+LLN+PE+LKK RDELD HVG DRLV ESDLPKLTYLK +I E
Sbjct: 300 LFAGTDSSAVTLEWSLSNLLNHPEMLKKARDELDFHVGKDRLVNESDLPKLTYLKKIILE 359
Query: 1386 TXXXXXXXXXXXXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEK 1565
T H +S++ II G+ VPRDTIVM N WA+ RDP +W+EAT+FKPERF++
Sbjct: 360 TLRLHPPAPLAIPHVSSEDVIIEGFNVPRDTIVMANIWAMHRDPAMWTEATSFKPERFDE 419
Query: 1566 EG 1571
EG
Sbjct: 420 EG 421
>dbj|BAT80225.1| hypothetical protein VIGAN_02322300 [Vigna angularis var. angularis]
Length = 499
Score = 522 bits (1344), Expect = e-179
Identities = 258/411 (62%), Positives = 320/411 (77%)
Frame = +3
Query: 339 FFCLALFFIFKLIFQSRKIKXXXXXXXXXXXXXXLHHLKRPLHRTFNRLSEKYGHVFSLW 518
FF L+L IFKL FQ+ K K L+ L+RPLHR R+S+ +GHVFSLW
Sbjct: 12 FFLLSLLIIFKLFFQT-KHKNLPPGPPPLPIIGNLNLLERPLHRFLQRMSQTHGHVFSLW 70
Query: 519 FGSRLVVVVSSPSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSVSYGELWRNLRRI 698
FGSRL V+VSSPS FQ+CFTKNDV LANRPR LSGK+IFYNYTT+GS SYGELWRNLRRI
Sbjct: 71 FGSRLAVIVSSPSAFQECFTKNDVALANRPRSLSGKHIFYNYTTVGSCSYGELWRNLRRI 130
Query: 699 TALDVLSNQRINSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISG 878
T+LDVLS QRI+SF+GIRKDET+R+I LA+ S ++ VE+SS F+DMT+N++MRM+SG
Sbjct: 131 TSLDVLSTQRIHSFAGIRKDETERVIHALAKASRMEYAHVEMSSMFHDMTYNSMMRMLSG 190
Query: 879 KRYYGEDLDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEK 1058
KRYYG+++ +L +A FR+ + E+LQL G +NK D++P L + DF+NLE KLK I+++
Sbjct: 191 KRYYGKEIQAQDLVKAKEFRETVEELLQLAGVSNKADYLPFLRWFDFQNLEKKLKSINKR 250
Query: 1059 NDAFLRGLLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVT 1238
D FL L+ EQR+KKER NTMIDHLL+LQESQP+YY+D IIKGL LAML AGTDSSAVT
Sbjct: 251 FDTFLDELIHEQRNKKERENTMIDHLLTLQESQPQYYSDHIIKGLVLAMLFAGTDSSAVT 310
Query: 1239 LDWSLSSLLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXX 1418
L+WSLS+LLN+PE+LKK RDEL+ HVG DRLV ESDLPKLTYLK +I ET
Sbjct: 311 LEWSLSNLLNHPEMLKKARDELNFHVGKDRLVNESDLPKLTYLKKIILETLRLHPPAPLA 370
Query: 1419 XXHSTSDECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1571
H +S++ II G+ VPRDT+VM N WA+ RDP W+EAT+FKPERF++EG
Sbjct: 371 IPHVSSEDVIIEGFNVPRDTLVMANIWAMHRDPATWTEATSFKPERFDEEG 421