BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus24_contig00002818
         (1401 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AHG97656.1| isoflavone-2'-hydroxylase [Astragalus mongholicus]     802   0.0  
ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   667   0.0  
ref|XP_017405614.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   666   0.0  
ref|XP_014506446.2| isoflavone 2'-hydroxylase [Vigna radiata var...   666   0.0  
gb|KOM25514.1| hypothetical protein LR48_Vigan107s003300 [Vigna ...   666   0.0  
ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phas...   661   0.0  
dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]                647   0.0  
sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase;...   645   0.0  
ref|NP_001296643.1| isoflavone 2'-hydroxylase-like [Cicer arieti...   645   0.0  
ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago ...   644   0.0  
dbj|BAT21113.1| cytochrome P450 [synthetic construct]                 641   0.0  
ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum] ...   642   0.0  
ref|XP_004504638.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   637   0.0  
ref|XP_004504637.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   635   0.0  
dbj|BAT21114.1| cytochrome P450 [synthetic construct]                 623   0.0  
dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]                     624   0.0  
ref|XP_016191270.1| isoflavone 2'-hydroxylase [Arachis ipaensis]      619   0.0  
ref|XP_015957959.1| isoflavone 2'-hydroxylase [Arachis duranensis]    619   0.0  
ref|XP_016201681.2| cytochrome P450 81E8 [Arachis ipaensis]           565   0.0  
gb|OIW14266.1| hypothetical protein TanjilG_21406 [Lupinus angus...   555   0.0  

>gb|AHG97656.1| isoflavone-2'-hydroxylase [Astragalus mongholicus]
          Length = 498

 Score =  802 bits (2072), Expect = 0.0
 Identities = 402/417 (96%), Positives = 402/417 (96%)
 Frame = -2

Query: 1253 NLIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVS 1074
            NLIFQSRKFK             NLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVS
Sbjct: 21   NLIFQSRKFKNLPPGPPSLPIIGNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVS 80

Query: 1073 SQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNR 894
            SQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITA DVLSTNR
Sbjct: 81   SQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNR 140

Query: 893  INSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDM 714
            INSFAGIRNDEIHRLVQKLA DSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDM
Sbjct: 141  INSFAGIRNDEIHRLVQKLAEDSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDM 200

Query: 713  TDVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIE 534
            TDVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIE
Sbjct: 201  TDVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIE 260

Query: 533  EHRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLN 354
            EHRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLN
Sbjct: 261  EHRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLN 320

Query: 353  NPEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECI 174
            NPEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECI
Sbjct: 321  NPEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECI 380

Query: 173  IAGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            IAGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP
Sbjct: 381  IAGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 437


>ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
 gb|KRH42431.1| hypothetical protein GLYMA_08G089500 [Glycine max]
          Length = 502

 Score =  667 bits (1720), Expect = 0.0
 Identities = 326/420 (77%), Positives = 367/420 (87%), Gaps = 4/420 (0%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+ Q+RKF+             NLHHLKRPL  TF+ LS+KYG ++SLWFGSRLVV+VSS
Sbjct: 22   LLLQARKFQNLPPGPPSLPIIGNLHHLKRPLHRTFRALSDKYGHVISLWFGSRLVVVVSS 81

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
            Q +FQECFTK+DVVLANRP+FLSGK+IFYNYTTLGSS YGEHWRNLRRITA DVLST+R+
Sbjct: 82   QTLFQECFTKNDVVLANRPRFLSGKHIFYNYTTLGSSPYGEHWRNLRRITALDVLSTHRL 141

Query: 890  NSFAGIRNDEIHRLVQKLA----ADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDD 723
            +SFA IR DE HRLV+KLA    ++SS  F EVELTSKFYDMTFNNIMRMISGKRYYGDD
Sbjct: 142  HSFAAIRRDETHRLVRKLAEAQGSESSLSFAEVELTSKFYDMTFNNIMRMISGKRYYGDD 201

Query: 722  CDMTDVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRG 543
            CDM DVEEA QFRAMVS+LLKL+GANNKNDF+P+LRL D+ENLEKRL KI +K D FLRG
Sbjct: 202  CDMADVEEAKQFRAMVSELLKLAGANNKNDFMPVLRLFDFENLEKRLKKISNKTDTFLRG 261

Query: 542  LIEEHRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSC 363
            L+EE R+KK+  NTM+DHLLSLQESQPEYYTDQIIKGL  G+L+A TDS AVTLEWALSC
Sbjct: 262  LLEEIRAKKQRANTMLDHLLSLQESQPEYYTDQIIKGLALGMLIAATDSQAVTLEWALSC 321

Query: 362  VLNNPEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSE 183
            VLN+PEV K+ RDEL+TH+GQD LLEESDL K+PYLKNI+YETLRLYTPAPL LPHSSSE
Sbjct: 322  VLNHPEVFKRARDELETHVGQDHLLEESDLSKLPYLKNIIYETLRLYTPAPLLLPHSSSE 381

Query: 182  ECIIAGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            ECII G+KVP DTI+L NAW+IHRDPKVWSEATSFKPERF+KEGEL+KLIAFGLGRRACP
Sbjct: 382  ECIIGGFKVPGDTIVLINAWSIHRDPKVWSEATSFKPERFEKEGELDKLIAFGLGRRACP 441


>ref|XP_017405614.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vigna angularis]
          Length = 500

 Score =  666 bits (1718), Expect = 0.0
 Identities = 320/416 (76%), Positives = 365/416 (87%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+ Q+RKF+             NLHHLKRPL  TFK LS+ YG ++SLWFGSRLVV+VSS
Sbjct: 24   LLLQARKFQNLPPGPPSLPIFGNLHHLKRPLHRTFKALSDNYGHVISLWFGSRLVVVVSS 83

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
             ++FQ CFTK+DV LANRP+FLSGKYIFYNYTTLGSS YG HWRNLRRITA ++LST+RI
Sbjct: 84   HSLFQHCFTKNDVALANRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTHRI 143

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            N+FA IR DE HRL++KLA D+S DF EVELTSKFYDMTFNNIMRMISGKRYYGDDCDM 
Sbjct: 144  NTFADIRRDETHRLIRKLAEDASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMA 203

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            DVEEA QFRAMVS+LLKLSGANNKNDF+P+LRL D+ENLEKRL KI  K D FL GL+EE
Sbjct: 204  DVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDFENLEKRLKKISSKTDTFLNGLLEE 263

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
             RS+K+  NTMVDHLLSLQESQP+YYTDQIIKGL  G+L+AGTDSSAVTLEWALSC+LN+
Sbjct: 264  QRSRKQGANTMVDHLLSLQESQPDYYTDQIIKGLALGMLVAGTDSSAVTLEWALSCMLNH 323

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PEVLK+TRDEL+  +GQ+RLLEESDLPK+PYLKNI++ETLRLYTPAPL LPHSSSEEC+I
Sbjct: 324  PEVLKRTRDELEKQVGQERLLEESDLPKLPYLKNIIFETLRLYTPAPLLLPHSSSEECMI 383

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             G+KVPRD+I+L NAW+IHRDP+VWSEATSFKPERF+ EGELEKLIAFG+GRRACP
Sbjct: 384  GGFKVPRDSIVLINAWSIHRDPEVWSEATSFKPERFESEGELEKLIAFGMGRRACP 439


>ref|XP_014506446.2| isoflavone 2'-hydroxylase [Vigna radiata var. radiata]
          Length = 504

 Score =  666 bits (1718), Expect = 0.0
 Identities = 320/416 (76%), Positives = 364/416 (87%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+ Q+RKF+             NLHHLKRPL  TFK LS+ YG ++SLWFGSRLVV+VSS
Sbjct: 28   LLLQARKFQNLPPGPPSLPVIGNLHHLKRPLHRTFKALSDNYGHVISLWFGSRLVVVVSS 87

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
             ++FQ CFTK+D+ LANRP+FLSGKYIFYNYTTLGSS YG HWRNLRRITA ++LST+RI
Sbjct: 88   HSLFQHCFTKNDIALANRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTHRI 147

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            N+FA IR DE HRL++KLA D+S DF EVELTSKFYDMTFNNIMRMISGKRYYGDDCDM 
Sbjct: 148  NTFADIRRDETHRLIRKLAEDASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMA 207

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            DVEEA QFRAMVS+LLKLSGANNKNDF+P+LRL DYENLEKRL KI  K D FL GL+EE
Sbjct: 208  DVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDYENLEKRLKKISSKTDTFLNGLLEE 267

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
             RSKK+  NTMVDHLLSLQESQP+YYTD+IIKGL  G+L+AGTDSSAVTLEWALSCVL +
Sbjct: 268  QRSKKQRANTMVDHLLSLQESQPDYYTDEIIKGLALGMLVAGTDSSAVTLEWALSCVLKH 327

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PEVLK+TRDEL+T +GQ+RLLEESD+ K+PYLKNI++ETLRLYTPAPL LPHSSSEECII
Sbjct: 328  PEVLKRTRDELETQVGQERLLEESDISKLPYLKNIIFETLRLYTPAPLLLPHSSSEECII 387

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             G+KVPRD+I+L NAW+IHRDP+VWSEATSFKPER++ EGELEKLIAFGLGRRACP
Sbjct: 388  GGFKVPRDSIVLINAWSIHRDPQVWSEATSFKPERYESEGELEKLIAFGLGRRACP 443


>gb|KOM25514.1| hypothetical protein LR48_Vigan107s003300 [Vigna angularis]
 dbj|BAT74198.1| hypothetical protein VIGAN_01181700 [Vigna angularis var. angularis]
          Length = 504

 Score =  666 bits (1718), Expect = 0.0
 Identities = 320/416 (76%), Positives = 365/416 (87%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+ Q+RKF+             NLHHLKRPL  TFK LS+ YG ++SLWFGSRLVV+VSS
Sbjct: 28   LLLQARKFQNLPPGPPSLPIFGNLHHLKRPLHRTFKALSDNYGHVISLWFGSRLVVVVSS 87

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
             ++FQ CFTK+DV LANRP+FLSGKYIFYNYTTLGSS YG HWRNLRRITA ++LST+RI
Sbjct: 88   HSLFQHCFTKNDVALANRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTHRI 147

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            N+FA IR DE HRL++KLA D+S DF EVELTSKFYDMTFNNIMRMISGKRYYGDDCDM 
Sbjct: 148  NTFADIRRDETHRLIRKLAEDASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMA 207

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            DVEEA QFRAMVS+LLKLSGANNKNDF+P+LRL D+ENLEKRL KI  K D FL GL+EE
Sbjct: 208  DVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDFENLEKRLKKISSKTDTFLNGLLEE 267

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
             RS+K+  NTMVDHLLSLQESQP+YYTDQIIKGL  G+L+AGTDSSAVTLEWALSC+LN+
Sbjct: 268  QRSRKQGANTMVDHLLSLQESQPDYYTDQIIKGLALGMLVAGTDSSAVTLEWALSCMLNH 327

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PEVLK+TRDEL+  +GQ+RLLEESDLPK+PYLKNI++ETLRLYTPAPL LPHSSSEEC+I
Sbjct: 328  PEVLKRTRDELEKQVGQERLLEESDLPKLPYLKNIIFETLRLYTPAPLLLPHSSSEECMI 387

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             G+KVPRD+I+L NAW+IHRDP+VWSEATSFKPERF+ EGELEKLIAFG+GRRACP
Sbjct: 388  GGFKVPRDSIVLINAWSIHRDPEVWSEATSFKPERFESEGELEKLIAFGMGRRACP 443


>ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
 gb|ESW30851.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
          Length = 500

 Score =  661 bits (1706), Expect = 0.0
 Identities = 317/416 (76%), Positives = 361/416 (86%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+ Q+R F+             NLHHLKRPL   FK LS+ YG ++SLWFGSRLVV+VSS
Sbjct: 24   LLLQARNFQNLPPGPPSLPIIGNLHHLKRPLHRHFKALSDNYGHVISLWFGSRLVVVVSS 83

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
             ++FQ CFTK+D+ LANRP+FLSGKYIFYNYTTLGSS YG HWRNLRRITA ++LST RI
Sbjct: 84   HSLFQHCFTKNDIALANRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTQRI 143

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            N+FA IR DE HRL++KLA  +S DF EVELTSKFYDMTFNNIMRMISGKRYYGDDCDM 
Sbjct: 144  NTFAAIRRDETHRLIRKLAEGASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMA 203

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            DVEEA QFRAMVS+LLKLSGANNKNDF+P+LRL D+ENLEKRL KI  K D FL GL+EE
Sbjct: 204  DVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDFENLEKRLKKISSKTDTFLNGLLEE 263

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
            HRSKK+  NTMVDHLLSLQESQPEYYTDQIIKGL  G+L+AGTDSSAVTLEWALSCV+N+
Sbjct: 264  HRSKKQRANTMVDHLLSLQESQPEYYTDQIIKGLALGMLIAGTDSSAVTLEWALSCVVNH 323

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PEV+K+ RDEL+TH+G++RLL+ESDL K+PYLKNI++ETLRLYTPAPL LPHSSSEEC I
Sbjct: 324  PEVMKRARDELETHVGEERLLDESDLSKLPYLKNIIFETLRLYTPAPLLLPHSSSEECNI 383

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             G+KVPRD+I+L NAW+IHRDP+VWSEATSFKPERF+ EGELEKLIAFGLGRRACP
Sbjct: 384  GGFKVPRDSIVLINAWSIHRDPEVWSEATSFKPERFENEGELEKLIAFGLGRRACP 439


>dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  647 bits (1670), Expect = 0.0
 Identities = 307/417 (73%), Positives = 367/417 (88%)
 Frame = -2

Query: 1253 NLIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVS 1074
            N++ ++RKFK             NLHHLKRPL  TFKGLSEKYG ++SLWFGSRLVV+VS
Sbjct: 21   NIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVISLWFGSRLVVVVS 80

Query: 1073 SQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNR 894
            S + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRITA DVLS +R
Sbjct: 81   SASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHR 140

Query: 893  INSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDM 714
            INSF+GIR DE  RL+ +LA DSS +F E+EL+ +FYDMTFNNIMRMISGKRYYG+DCDM
Sbjct: 141  INSFSGIRRDETQRLITRLADDSSTNFAEIELSYRFYDMTFNNIMRMISGKRYYGEDCDM 200

Query: 713  TDVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIE 534
            +D++EA+QFR MVS+LL+LSGANNK DF+P+LR +D+ENLEKRL  I  K DAFLRGLI+
Sbjct: 201  SDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIQ 260

Query: 533  EHRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLN 354
            EHR+KK+  NTM+DHLL+LQ+SQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++S +LN
Sbjct: 261  EHRAKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLN 320

Query: 353  NPEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECI 174
            +PEVLKK +DELDTH+GQDRL++ESDLPK+ YLKN++ ETLRLYTPAPL LPHS+S+EC 
Sbjct: 321  HPEVLKKVKDELDTHVGQDRLVDESDLPKLSYLKNVINETLRLYTPAPLLLPHSTSDECN 380

Query: 173  IAGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            I GYKVP+DTI+L NAWAIHRDP++W+EAT+FKPERF+K+GELEKLIAFG+GRRACP
Sbjct: 381  IGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELEKLIAFGMGRRACP 437


>sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase; AltName: Full=CYP GE-3;
            AltName: Full=Cytochrome P450 81E1; AltName:
            Full=Cytochrome P450 91A4
 dbj|BAA22422.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  645 bits (1664), Expect = 0.0
 Identities = 307/417 (73%), Positives = 365/417 (87%)
 Frame = -2

Query: 1253 NLIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVS 1074
            N++ ++RKFK             NLHHLKRPL  TFKGLSEKYG + SLWFGSRLVV+VS
Sbjct: 21   NIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVS 80

Query: 1073 SQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNR 894
            S + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRITA DVLS +R
Sbjct: 81   SASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHR 140

Query: 893  INSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDM 714
            INSF+GIR DE  RL+ +LA DSS +F E+EL+S+ YDMTFNNIMRMISGKRYYG+DCD 
Sbjct: 141  INSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDT 200

Query: 713  TDVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIE 534
            +D++EA+QFR MVS+LL+LSGANNK DF+P+LR +D+ENLEKRL  I  K DAFLRGLIE
Sbjct: 201  SDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIE 260

Query: 533  EHRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLN 354
            EHR+KK+  NTM+DHLL+LQ+SQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++S +LN
Sbjct: 261  EHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLN 320

Query: 353  NPEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECI 174
            +PEVLKK +DELDTH+GQDRL++ESDLPK+ YLKN++ ETLRLYTPAPL LPHS+S+EC 
Sbjct: 321  HPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECN 380

Query: 173  IAGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            I GYKVP+DTI+L NAWAIHRDP++W+EAT+FKPERF+K+GELEKLIAFG+GRRACP
Sbjct: 381  IGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELEKLIAFGMGRRACP 437


>ref|NP_001296643.1| isoflavone 2'-hydroxylase-like [Cicer arietinum]
 emb|CAB41490.1| cytochrome P450 monooxygenase [Cicer arietinum]
          Length = 498

 Score =  645 bits (1663), Expect = 0.0
 Identities = 306/416 (73%), Positives = 362/416 (87%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+FQSRKFK             N HHLKRPL  TFKGLS+ YGDI+SLWFGSRLVV+VSS
Sbjct: 22   LLFQSRKFKNLPPGPPSLPIIGNFHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSS 81

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
             + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS+SY EHWRNLRRIT+ DVLS +RI
Sbjct: 82   LSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRI 141

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            N+F+G+R DE  RL+ KLA DSS  F EVEL+ +FYDMTFNNIMRMISGKRYYG+DCDM+
Sbjct: 142  NNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMTFNNIMRMISGKRYYGEDCDMS 201

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            D++EA+QFR MV++LL+LSGANNK DF+P+L+  D+ENLEKR+  I DK DAFLRGL++E
Sbjct: 202  DLQEASQFRDMVTELLQLSGANNKTDFMPLLKFFDFENLEKRVKNIADKTDAFLRGLLQE 261

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
             R+KK+  NTM+DHLL+LQESQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++S +LN+
Sbjct: 262  QRNKKERTNTMIDHLLNLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNH 321

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PEVLKK RDELDTH+GQDRL++ESDLPK+ YLKN++YETLRL TPAPL LPHS+S++CII
Sbjct: 322  PEVLKKVRDELDTHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCII 381

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             GYKVPRDTI+L NAWAIHRDPK WSEAT+FKPERFDK+GE+EK+IAFG+GRR CP
Sbjct: 382  GGYKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCP 437


>ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago truncatula]
 sp|Q6WNR0.1|C81E7_MEDTR RecName: Full=Isoflavone 2'-hydroxylase; AltName:
            Full=4'-methoxyisoflavone 2'-hydroxylase; AltName:
            Full=Cytochrome P450 81E7
 gb|AAQ20040.1| isoflavone 2'-hydroxylase [Medicago truncatula]
 gb|KEH31433.1| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 498

 Score =  644 bits (1662), Expect = 0.0
 Identities = 305/416 (73%), Positives = 364/416 (87%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+FQSRKFK             NLHHLKRPL  TFK L+EKYG+++SLWFGSRLVV+VSS
Sbjct: 22   LLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWFGSRLVVVVSS 81

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
             + FQECFTK+DVVLANRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRIT+ DVLS +RI
Sbjct: 82   LSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRI 141

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            N+FA IR DE  RL++KLA DSS  F EVELT +F+DMTFNNIMRMISGKRYYGDDCD++
Sbjct: 142  NNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMISGKRYYGDDCDIS 201

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            +V+EA+QFR MVS+LL+LSGANNK DF+P+L+ +D+ENLEKR+ +I +KND FL GL++E
Sbjct: 202  EVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRIGEKNDVFLSGLLQE 261

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
             RSKK+  NTM+DHLL++QESQPEYYTD IIKGL   +LLAGTDSSAVTLEW +S +LN 
Sbjct: 262  QRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAGTDSSAVTLEWTMSNILNY 321

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PEVLKK RDE+DTH+GQDRL++ESDLPK+ YL+N++YETLRLYTPAPL LPHS+++ECI+
Sbjct: 322  PEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLYTPAPLLLPHSTADECIM 381

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             GYKVPRDTI+L NAWAIHRDP+ WSEAT+FKPERFDK+GELEK+IAFG+GRRACP
Sbjct: 382  GGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKGELEKMIAFGMGRRACP 437


>dbj|BAT21113.1| cytochrome P450 [synthetic construct]
          Length = 477

 Score =  641 bits (1654), Expect = 0.0
 Identities = 306/415 (73%), Positives = 363/415 (87%)
 Frame = -2

Query: 1247 IFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSSQ 1068
            + ++RKFK             NLHHLKRPL  TFKGLSEKYG + SLWFGSRLVV+VSS 
Sbjct: 1    MIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSA 60

Query: 1067 AIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRIN 888
            + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRITA DVLS +RIN
Sbjct: 61   SEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRIN 120

Query: 887  SFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTD 708
            SF+GIR DE  RL+ +LA DSS +F E+EL+S+ YDMTFNNIMRMISGKRYYG+DCD +D
Sbjct: 121  SFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSD 180

Query: 707  VEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEH 528
            ++EA+QFR MVS+LL+LSGANNK DF+P+LR +D+ENLEKRL  I  K DAFLRGLIEEH
Sbjct: 181  LQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEH 240

Query: 527  RSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNP 348
            R+KK+  NTM+DHLL+LQ+SQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++S +LN+P
Sbjct: 241  RTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHP 300

Query: 347  EVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIA 168
            EVLKK +DELDTH+GQDRL++ESDLPK+ YLKN++ ETLRLYTPAPL LPHS+S+EC I 
Sbjct: 301  EVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIG 360

Query: 167  GYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            GYKVP+DTI+L NAWAIHRDP++W+EAT+FKPERF+K+GELEKLIAFG+GRRACP
Sbjct: 361  GYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELEKLIAFGMGRRACP 415


>ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum]
 emb|CAA10067.1| cytochrome P450 [Cicer arietinum]
          Length = 498

 Score =  642 bits (1655), Expect = 0.0
 Identities = 304/416 (73%), Positives = 361/416 (86%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+FQSRKFK             NLHHLKRPL  TFKGLS+ YGDI+SLWFGSRLVV+VSS
Sbjct: 22   LLFQSRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSS 81

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
             + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS+SY EHWRNLRRIT+ DVLS +RI
Sbjct: 82   LSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRI 141

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            N+F+G+R DE  RL+ KLA DSS  F EVEL+ +FYDMTFNNIMRMI GKRYYG+DCDM+
Sbjct: 142  NNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMTFNNIMRMIPGKRYYGEDCDMS 201

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            D++EA+QFR MV++LL+LSGANNK DF+P+L+  D+ENLEKR+  I DK DAFLRGL++E
Sbjct: 202  DLQEASQFRDMVTELLQLSGANNKTDFMPLLKFFDFENLEKRVKNIADKTDAFLRGLLQE 261

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
             R+KK+  NTM+DHLL+LQESQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++S +LN+
Sbjct: 262  QRNKKERTNTMIDHLLNLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNH 321

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PE+LKK +DELD H+GQDRL++ESDLPK+ YLKN++YETLRL TPAPL LPHS+S++CII
Sbjct: 322  PEILKKVKDELDIHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCII 381

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             GYKVPRDTI+L NAWAIHRDPK WSEATSFKPERFDK+GE+EK+IAFG+GRR CP
Sbjct: 382  GGYKVPRDTIVLINAWAIHRDPKSWSEATSFKPERFDKKGEIEKVIAFGMGRRVCP 437


>ref|XP_004504638.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
          Length = 498

 Score =  637 bits (1643), Expect = 0.0
 Identities = 301/416 (72%), Positives = 360/416 (86%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+FQSRKFK             NLHHLKRPL  TFKGLS+ YGDI+SLWFGSRLVV+VSS
Sbjct: 22   LLFQSRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSS 81

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
             + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS+SY EHWRNLRRIT+ DVLS +RI
Sbjct: 82   LSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRI 141

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            N+F+G+R DE  RL+ KLA DSS  F EVEL+ +FYDMTFNNIMRMISGKRYYG+DCDM+
Sbjct: 142  NNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMTFNNIMRMISGKRYYGEDCDMS 201

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            D++EA+QFR MV++LL+LSGANNK DFLP+L+  D+ENLEKR+  I +K D FLRGL++E
Sbjct: 202  DLQEASQFRDMVTELLQLSGANNKTDFLPLLKFFDFENLEKRVKNIANKTDTFLRGLLQE 261

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
             R+KK+  NTM+DHLL+LQESQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++  ++N+
Sbjct: 262  QRNKKERTNTMIDHLLNLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNLMNH 321

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PE+LKK +DELD H+GQDRL++ESDLPK+ YLKN++YETLRL TPAPL LPHS+S++CII
Sbjct: 322  PEILKKVKDELDIHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCII 381

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             GYKVPRDTI+L NAWAIHRDPK WSEAT+FKPERFDK+GE+EK+IAFG+GRR CP
Sbjct: 382  GGYKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCP 437


>ref|XP_004504637.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
          Length = 511

 Score =  635 bits (1639), Expect = 0.0
 Identities = 298/416 (71%), Positives = 365/416 (87%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+FQSR+FK             NLHHLKRPL  TFK L++KYGD++SLWFGSRLVV+VSS
Sbjct: 22   LLFQSRRFKNLPPGPPSLPIIGNLHHLKRPLHRTFKELTKKYGDVISLWFGSRLVVVVSS 81

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
             + FQ+CFTK+DVVLANRPKF+SGKYIFYNYTTLGS+SYGEHWRNLRRIT+ DVLS +RI
Sbjct: 82   LSEFQQCFTKNDVVLANRPKFISGKYIFYNYTTLGSASYGEHWRNLRRITSLDVLSNHRI 141

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            N+F+GIR DE  RL++KLA DSS +F EVEL+ +FYDMTFNNIMRMISGKRYYG+DCDM+
Sbjct: 142  NNFSGIRKDETQRLIKKLAEDSSSNFAEVELSFRFYDMTFNNIMRMISGKRYYGEDCDMS 201

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            D++EA+QFR MV++LL+LSGANNK DFLP+LR +D+ENLEK++  I +K DAFL GL++E
Sbjct: 202  DLQEASQFRDMVTELLQLSGANNKTDFLPLLRFLDFENLEKKVKNIGEKTDAFLSGLLQE 261

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
             R+KK+  NTM+DHLLS+QESQPEYYTDQIIKGLV  +LLAGTD++A+TLEW++S +LN+
Sbjct: 262  QRNKKERTNTMIDHLLSMQESQPEYYTDQIIKGLVLAMLLAGTDTTALTLEWSMSNLLNH 321

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PEVLKK RDELDTH+GQDRL++ESDLP + Y+KN++YETLRLY PAPL +PHS+S++CII
Sbjct: 322  PEVLKKVRDELDTHVGQDRLVDESDLPNLTYIKNVIYETLRLYGPAPLLIPHSTSDDCII 381

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             GYK+PRDTI+L NAW I RDP+ WSEAT+FKPERF+K+GELEKLIAFG+GRRACP
Sbjct: 382  GGYKIPRDTIVLINAWGIQRDPESWSEATTFKPERFNKKGELEKLIAFGMGRRACP 437


>dbj|BAT21114.1| cytochrome P450 [synthetic construct]
          Length = 477

 Score =  623 bits (1607), Expect = 0.0
 Identities = 301/413 (72%), Positives = 357/413 (86%), Gaps = 1/413 (0%)
 Frame = -2

Query: 1238 SRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSSQAIF 1059
            SRKFK             NLHHLKRPL  TF+ LSEKYGD+ SLWFG+RLVV+VSS A  
Sbjct: 4    SRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSSFADV 63

Query: 1058 QECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRINSFA 879
            QECFTK+DVVLANRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRIT+ DVLS +RINSF+
Sbjct: 64   QECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRINSFS 123

Query: 878  GIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEE 699
             IR DE  RL++KLA DS+++F EVELTS+F+DMTFNNIMRMISGKRYYG+DCDMT+++E
Sbjct: 124  PIRRDETTRLIRKLAEDSAKNFSEVELTSRFFDMTFNNIMRMISGKRYYGEDCDMTELQE 183

Query: 698  ATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSK 519
            A++FR MV++LL+LSGANNK DF+PILRLVD+E LEKR+  I  K D FLRGL++EHR K
Sbjct: 184  ASEFRDMVTELLQLSGANNKADFMPILRLVDFEGLEKRVKGISSKTDRFLRGLLQEHRDK 243

Query: 518  KKHD-NTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEV 342
            K+   NTM+DHLL+LQESQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++  VLN PEV
Sbjct: 244  KQRTANTMIDHLLTLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNVLNYPEV 303

Query: 341  LKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGY 162
            L+K + ELDTH+GQDRL++ESD+PK+ YLKN++ ETLRLYTPAPL LPHS+S++C I GY
Sbjct: 304  LEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCTIGGY 363

Query: 161  KVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            KVPRDTI+L NAWA+HRDP++W+EAT+FKPERFDK+GELEKLI FGLGRRACP
Sbjct: 364  KVPRDTIVLINAWALHRDPQLWTEATTFKPERFDKKGELEKLIPFGLGRRACP 416


>dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]
          Length = 499

 Score =  624 bits (1609), Expect = 0.0
 Identities = 302/417 (72%), Positives = 358/417 (85%), Gaps = 1/417 (0%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L   SRKFK             NLHHLKRPL  TF+ LSEKYGD+ SLWFG+RLVV+VSS
Sbjct: 22   LFLGSRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSS 81

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
             A  QECFTK+DVVLANRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRIT+ DVLS +RI
Sbjct: 82   FADVQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRI 141

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            NSF+ IR DE  RL++KLA DS+++F EVELTS+F+DMTFNNIMRMISGKRYYG+DCDMT
Sbjct: 142  NSFSPIRRDETTRLIRKLAEDSAKNFSEVELTSRFFDMTFNNIMRMISGKRYYGEDCDMT 201

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            +++EA++FR MV++LL+LSGANNK DF+PILRLVD+E LEKR+  I  K D FLRGL++E
Sbjct: 202  ELQEASEFRDMVTELLQLSGANNKADFMPILRLVDFEGLEKRVKGISSKTDRFLRGLLQE 261

Query: 530  HRSKKKHD-NTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLN 354
            HR KK+   NTM+DHLL+LQESQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++  VLN
Sbjct: 262  HRDKKQRTANTMIDHLLTLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNVLN 321

Query: 353  NPEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECI 174
             PEVL+K + ELDTH+GQDRL++ESD+PK+ YLKN++ ETLRLYTPAPL LPHS+S++C 
Sbjct: 322  YPEVLEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCT 381

Query: 173  IAGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            I GYKVPRDTI+L NAWA+HRDP++W+EAT+FKPERFDK+GELEKLI FGLGRRACP
Sbjct: 382  IGGYKVPRDTIVLINAWALHRDPQLWTEATTFKPERFDKKGELEKLIPFGLGRRACP 438


>ref|XP_016191270.1| isoflavone 2'-hydroxylase [Arachis ipaensis]
          Length = 499

 Score =  619 bits (1597), Expect = 0.0
 Identities = 290/416 (69%), Positives = 352/416 (84%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+ QSR FK             NLHHLK+PL   F+GLSEKYGD+ SLWFGSRLVV+VSS
Sbjct: 22   LLIQSRSFKNLPPGPPSLPIIGNLHHLKKPLHRHFRGLSEKYGDVFSLWFGSRLVVVVSS 81

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
              + QECFTK+DVVLANRP+FLSGKYIFYNYTT+GSS YGEHWRNLRRIT+ DVLS +R+
Sbjct: 82   PTVVQECFTKNDVVLANRPRFLSGKYIFYNYTTVGSSEYGEHWRNLRRITSLDVLSNHRL 141

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            NSF+ IR DE  RL++KL+ DS+E+F +VEL+SKFYDMTFNNIMRMISGKRYYG+DCDM 
Sbjct: 142  NSFSPIRRDETTRLIRKLSEDSTENFAKVELSSKFYDMTFNNIMRMISGKRYYGEDCDMA 201

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            DVEEA QFR M+S++L+LSGANNK DF+P+L ++D +NL+KRLV I  + D FL GL++E
Sbjct: 202  DVEEAKQFRQMISEVLQLSGANNKTDFIPLLGMLDVDNLKKRLVTISGRTDKFLNGLLQE 261

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
            HR+K +H NTM+DHLLSLQ+SQP+YYTDQIIKGL   +LLAGTDSSAVTLEW++  +LN+
Sbjct: 262  HRTKNQHQNTMIDHLLSLQDSQPDYYTDQIIKGLGLAMLLAGTDSSAVTLEWSMCNLLNH 321

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PE L + R+ELDTH+GQDR+++ESD+PK  YL+N++ ETLRLYTPAPL LPHSS+E+C I
Sbjct: 322  PEALNRVREELDTHVGQDRIVDESDIPKFTYLRNVINETLRLYTPAPLLLPHSSAEKCTI 381

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             GY +P+DTI+L N WAIHRDPK W EATSFKPERF+++GELEKLIAFGLGRRACP
Sbjct: 382  GGYNIPKDTIVLINGWAIHRDPKNWDEATSFKPERFERKGELEKLIAFGLGRRACP 437


>ref|XP_015957959.1| isoflavone 2'-hydroxylase [Arachis duranensis]
          Length = 499

 Score =  619 bits (1597), Expect = 0.0
 Identities = 290/416 (69%), Positives = 352/416 (84%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+ QSR FK             NLHHLK+PL   F+GLSEKYGD+ SLWFGSRLVV+VSS
Sbjct: 22   LLIQSRSFKNLPPGPPSLPIIGNLHHLKKPLHRHFRGLSEKYGDVFSLWFGSRLVVVVSS 81

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
              + QECFTK+DVVLANRP+FLSGKYIFYNYTT+GSS YGEHWRNLRRIT+ DVLS +R+
Sbjct: 82   PTVVQECFTKNDVVLANRPRFLSGKYIFYNYTTVGSSEYGEHWRNLRRITSLDVLSNHRL 141

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            NSF+ IR DE  RL++KL+ DS+E+F +VEL+SKFYDMTFNNIMRMISGKRYYG+DCDM 
Sbjct: 142  NSFSPIRRDETTRLIRKLSEDSTENFAKVELSSKFYDMTFNNIMRMISGKRYYGEDCDMA 201

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            DVEEA  FR M+S++L+LSGANNK DF+P+L ++D +NL+KRLV I  + D FL GL++E
Sbjct: 202  DVEEAKHFRQMISEVLQLSGANNKTDFIPLLGMLDVDNLKKRLVTISGRTDKFLNGLLQE 261

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
            HR+K +H NTM+DHLLSLQ+SQP+YYTDQIIKGL   +LLAGTDSSAVTLEW++  +LN+
Sbjct: 262  HRTKNQHQNTMIDHLLSLQDSQPDYYTDQIIKGLGLAMLLAGTDSSAVTLEWSMCNLLNH 321

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PE L + R+ELDTH+GQDR+++ESD+PK  YL+N++ ETLRLYTPAPL LPHSS+E+C I
Sbjct: 322  PEALNRVREELDTHVGQDRIVDESDIPKFTYLRNVINETLRLYTPAPLLLPHSSAEKCTI 381

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             GYK+P+DTI+L N WAIHRDPK W EATSFKPERF+++GELEKLIAFGLGRRACP
Sbjct: 382  GGYKIPKDTIVLINGWAIHRDPKNWDEATSFKPERFERKGELEKLIAFGLGRRACP 437


>ref|XP_016201681.2| cytochrome P450 81E8 [Arachis ipaensis]
          Length = 499

 Score =  565 bits (1457), Expect = 0.0
 Identities = 269/417 (64%), Positives = 337/417 (80%), Gaps = 1/417 (0%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+F+SR  K             NLHHLK+PL   F+GLSEKYGD+ SLWFGSRLVV+VSS
Sbjct: 21   LLFKSRALKNLPPGPPYLPLIGNLHHLKKPLHRHFRGLSEKYGDVFSLWFGSRLVVVVSS 80

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
              + QECFTK+DVVLANRP+FLSGKYIFYNYTT+GSS YGEHWRNLRRI   +VLST+RI
Sbjct: 81   PTVVQECFTKNDVVLANRPRFLSGKYIFYNYTTVGSSEYGEHWRNLRRIATLEVLSTHRI 140

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
            NSF  IR DEI RLVQKLA + S  F +VEL SK  +MTFN IMRM+SGKRYYG+DCD+T
Sbjct: 141  NSFLEIRRDEIMRLVQKLAQECSNGFNKVELRSKLSEMTFNTIMRMVSGKRYYGEDCDVT 200

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            DVEEA +FRA+  +L+ L GANN  DF+ +LR  D++ LE +L +I ++ D+FL+GLI+E
Sbjct: 201  DVEEARRFRAITKELVALGGANNPGDFISVLRWFDFDGLENKLKRISNRTDSFLQGLIDE 260

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
            HR+ K++ NTM+DHLL+ Q+S PEYYTDQIIKGL+  +LLAGTD+S+VTLEWA+S +LN+
Sbjct: 261  HRNGKRNTNTMIDHLLTQQQSHPEYYTDQIIKGLIMVMLLAGTDTSSVTLEWAMSNLLNH 320

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PE+L++ R E+DT IGQD L++ESD+ K+ YL+NIVYET RL+  APL +PH SSE+C +
Sbjct: 321  PEILERARKEIDTEIGQDHLIDESDISKLNYLQNIVYETFRLHPAAPLLVPHLSSEDCTL 380

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWS-EATSFKPERFDKEGELEKLIAFGLGRRACP 3
             GYKVP++TI+L NAWAIHRDPKVWS ++T FKPERF+KEGE+  L+ FG+GRRACP
Sbjct: 381  EGYKVPQNTIVLVNAWAIHRDPKVWSDDSTQFKPERFEKEGEINNLLTFGIGRRACP 437


>gb|OIW14266.1| hypothetical protein TanjilG_21406 [Lupinus angustifolius]
          Length = 498

 Score =  555 bits (1430), Expect = 0.0
 Identities = 264/416 (63%), Positives = 329/416 (79%)
 Frame = -2

Query: 1250 LIFQSRKFKXXXXXXXXXXXXXNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 1071
            L+F++++FK             NL+ L++PL   F+ +S+K+GD+ SLWFGSRL V++SS
Sbjct: 22   LLFKTKRFKNLPPGPPPLPIIGNLNLLEKPLHRFFQTMSKKHGDVFSLWFGSRLAVVISS 81

Query: 1070 QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITAADVLSTNRI 891
               FQECFTKHDV+LANRPK LSGK+IFYNYTT+GS SYGEHWRNLRRI A DVLS+ RI
Sbjct: 82   PTAFQECFTKHDVILANRPKNLSGKHIFYNYTTVGSCSYGEHWRNLRRIVATDVLSSQRI 141

Query: 890  NSFAGIRNDEIHRLVQKLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMT 711
             SF+GIR DE HRLVQKLA DSS  F +VELTS F DMT+NN+MRMI+GKRYYG+D ++ 
Sbjct: 142  LSFSGIRKDETHRLVQKLAKDSSSKFAQVELTSTFRDMTYNNMMRMIAGKRYYGEDSELK 201

Query: 710  DVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEE 531
            DV+EA +FR  V++LLKL+G +NK D+LP L+  D+E L K+L     + D F+  LI+E
Sbjct: 202  DVDEAKEFRETVTELLKLAGVSNKADYLPFLKWFDFEGLNKKLTTTHKRFDVFVSALIKE 261

Query: 530  HRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNN 351
             RSKK+ +++M+DHLL+LQE+QP+YYTDQ+IKGL   +L AGTDSS  TLEWA   +LNN
Sbjct: 262  QRSKKQREDSMIDHLLTLQETQPDYYTDQVIKGLTLAMLFAGTDSSGATLEWAFCNLLNN 321

Query: 350  PEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECII 171
            PEVLKK +DELDTHIGQDRLL ESDLPK+PYLK IV ETLRLY PAPL +PH SSE+  +
Sbjct: 322  PEVLKKAKDELDTHIGQDRLLTESDLPKLPYLKYIVQETLRLYPPAPLLIPHVSSEDITV 381

Query: 170  AGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             G+ +P DT+++ N W + RDPK WS+ATSFKPERF+KEGE +KLIAFGLGRRACP
Sbjct: 382  EGFNIPSDTLVIVNGWGMQRDPKFWSDATSFKPERFEKEGEEKKLIAFGLGRRACP 437