BLASTX nr result
ID: Astragalus23_contig00033872
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Astragalus23_contig00033872
(469 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|PNX74474.1| cytochrome p450 [Trifolium pratense] 161 6e-47
gb|PNX61869.1| hypothetical protein L195_g060877, partial [Trifo... 128 3e-35
ref|XP_022139673.1| uncharacterized protein LOC111010521 [Momord... 128 1e-31
gb|PON95853.1| hypothetical protein TorRG33x02_083760, partial [... 117 2e-30
gb|PON75591.1| hypothetical protein PanWU01x14_042160 [Parasponi... 115 1e-29
gb|POO02710.1| hypothetical protein TorRG33x02_016960, partial [... 112 8e-29
ref|XP_022876702.1| nucleolar protein 14-like [Olea europaea var... 115 3e-28
gb|PON70119.1| hypothetical protein PanWU01x14_082940 [Parasponi... 105 5e-26
gb|PHT69902.1| hypothetical protein T459_25006 [Capsicum annuum] 108 5e-25
ref|XP_016576563.1| PREDICTED: peptide methionine sulfoxide redu... 103 1e-24
gb|PON43110.1| hypothetical protein PanWU01x14_276620 [Parasponi... 101 1e-24
gb|ESR55658.1| hypothetical protein CICLE_v10023471mg, partial [... 100 3e-24
gb|PON56879.1| hypothetical protein TorRG33x02_294980 [Trema ori... 99 3e-24
gb|PON66942.1| Pol-like polyprotein/retrotransposon [Trema orien... 98 2e-23
ref|XP_013443246.1| hypothetical protein MTR_0027s0030 [Medicago... 96 8e-23
gb|PNX62013.1| F-box/kelch-repeat protein, partial [Trifolium pr... 94 2e-22
gb|PON68556.1| hypothetical protein PanWU01x14_095270 [Parasponi... 96 2e-22
ref|XP_018827443.1| PREDICTED: uncharacterized protein LOC108996... 93 8e-21
gb|KJB48829.1| hypothetical protein B456_008G089200 [Gossypium r... 90 8e-20
gb|KGN63177.1| hypothetical protein Csa_2G406750 [Cucumis sativus] 93 1e-19
>gb|PNX74474.1| cytochrome p450 [Trifolium pratense]
Length = 224
Score = 161 bits (407), Expect = 6e-47
Identities = 81/109 (74%), Positives = 89/109 (81%), Gaps = 2/109 (1%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQS--KHGLKGNISNMNKEKWEEVDLRAASAIHLCLAKNVL 221
KFDGRINFDLWK+QVNDVLIQS LKGN S M +KWEE+DLRAASAI LCLAKNVL
Sbjct: 13 KFDGRINFDLWKVQVNDVLIQSGLHKALKGNTSKMXXDKWEELDLRAASAIRLCLAKNVL 72
Query: 220 ANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEKFHNLRMDEDMKVSDH 74
ANV + S K+ WE+LE LYQAK I NRLLLKE+FHNLRMDE K+SDH
Sbjct: 73 ANVYNLSSAKELWERLERLYQAKDISNRLLLKEQFHNLRMDEGTKISDH 121
>gb|PNX61869.1| hypothetical protein L195_g060877, partial [Trifolium pratense]
Length = 118
Score = 128 bits (321), Expect = 3e-35
Identities = 65/90 (72%), Positives = 72/90 (80%), Gaps = 2/90 (2%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQS--KHGLKGNISNMNKEKWEEVDLRAASAIHLCLAKNVL 221
KF+GRINF LWK+QVNDV IQS LKGN S M +KWEE+DLRAASAI LCLAKNVL
Sbjct: 29 KFNGRINFGLWKVQVNDVFIQSGLHKALKGNTSKMEVDKWEELDLRAASAIRLCLAKNVL 88
Query: 220 ANVQEILSTKDFWEKLEGLYQAKGI*NRLL 131
ANVQ + S K+ WE+LEGLYQAK I NRLL
Sbjct: 89 ANVQNLSSAKELWERLEGLYQAKDISNRLL 118
>ref|XP_022139673.1| uncharacterized protein LOC111010521 [Momordica charantia]
Length = 564
Score = 128 bits (322), Expect = 1e-31
Identities = 72/136 (52%), Positives = 85/136 (62%), Gaps = 29/136 (21%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQSK--HGLKG---------------------------NIS 302
KFDG INF LW++QV DVLIQS+ LKG S
Sbjct: 308 KFDGMINFGLWQVQVKDVLIQSELHKALKGRPSEGASEKLSDDGGPMESSGGSSRGSKKS 367
Query: 301 NMNKEKWEEVDLRAASAIHLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKE 122
+M+ E WEE+DLRAASAI LAKN+LANV I + K+ WEKLE LYQAKGI NRL LKE
Sbjct: 368 SMSYEDWEEMDLRAASAIRTSLAKNILANVHRISTAKELWEKLEALYQAKGISNRLYLKE 427
Query: 121 KFHNLRMDEDMKVSDH 74
+FH L+M+E MK+SDH
Sbjct: 428 QFHTLQMEEGMKISDH 443
>gb|PON95853.1| hypothetical protein TorRG33x02_083760, partial [Trema orientalis]
Length = 156
Score = 117 bits (292), Expect = 2e-30
Identities = 64/113 (56%), Positives = 80/113 (70%), Gaps = 6/113 (5%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQS--KHGLKGNI----SNMNKEKWEEVDLRAASAIHLCLA 233
KFDG INFDL ++QV D+LIQS LKG S ++ E WE++D RAASAI +CLA
Sbjct: 16 KFDGNINFDLCQVQVKDLLIQSGLHKVLKGRDNSGKSKISDEDWEDLDERAASAIWMCLA 75
Query: 232 KNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEKFHNLRMDEDMKVSDH 74
KNVLANV I + KD W+KL LY+AK + NR+ LKE+FH LRM E + +SDH
Sbjct: 76 KNVLANVLGISTAKDLWKKLGELYRAKDVSNRVYLKEQFHTLRMSEGIIISDH 128
>gb|PON75591.1| hypothetical protein PanWU01x14_042160 [Parasponia andersonii]
Length = 161
Score = 115 bits (287), Expect = 1e-29
Identities = 64/118 (54%), Positives = 81/118 (68%), Gaps = 11/118 (9%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQS--KHGLKGNI---------SNMNKEKWEEVDLRAASAI 248
KF G INF LW+IQV ++LIQS LKG S+M+ E +++D RAAS I
Sbjct: 16 KFGGNINFGLWQIQVKNLLIQSGLHKILKGREAYKRRDSQKSSMSDEDLDDLDERAASMI 75
Query: 247 HLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEKFHNLRMDEDMKVSDH 74
++CLAK VLANV ++ KDFW KLE LYQAKG+ NR+ LKE+FH LRM+E K+SDH
Sbjct: 76 YMCLAKIVLANVLGKMTAKDFWRKLEELYQAKGVSNRVYLKEQFHTLRMNEGTKISDH 133
>gb|POO02710.1| hypothetical protein TorRG33x02_016960, partial [Trema orientalis]
Length = 152
Score = 112 bits (281), Expect = 8e-29
Identities = 58/113 (51%), Positives = 78/113 (69%), Gaps = 6/113 (5%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQS--KHGLKGNI----SNMNKEKWEEVDLRAASAIHLCLA 233
KFDG INF LW +QV D+LIQS + LKG S ++ E W+++D RAASAI +CLA
Sbjct: 14 KFDGNINFGLWHVQVRDLLIQSGLHNILKGRDKFGKSKISDEDWKDLDERAASAIRMCLA 73
Query: 232 KNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEKFHNLRMDEDMKVSDH 74
KN+L N+ I + KD WEKL LY+AKG+ NR+ LK++F+ L M + +SDH
Sbjct: 74 KNILVNMLGISTAKDLWEKLRKLYRAKGVSNRVYLKKQFYTLHMSKGTTISDH 126
>ref|XP_022876702.1| nucleolar protein 14-like [Olea europaea var. sylvestris]
Length = 1147
Score = 115 bits (289), Expect(2) = 3e-28
Identities = 59/118 (50%), Positives = 78/118 (66%), Gaps = 11/118 (9%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQS-----------KHGLKGNISNMNKEKWEEVDLRAASAI 248
KFD +I F LW++QV D+LIQ+ K S+M+ E WEE+DL+AAS I
Sbjct: 10 KFDRKIKFGLWQVQVRDLLIQTGLHKALKRKSTKSYCNEAKSSMSNEDWEELDLKAASII 69
Query: 247 HLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEKFHNLRMDEDMKVSDH 74
LCLAKNVL N+ I +TK+ WEKLE LYQ K + NRL LKE+FH+L ++ K+S+H
Sbjct: 70 RLCLAKNVLTNIHRISATKELWEKLEELYQTKSLSNRLYLKEQFHSLLLEAGTKISNH 127
Score = 37.0 bits (84), Expect(2) = 3e-28
Identities = 18/22 (81%), Positives = 18/22 (81%)
Frame = -2
Query: 66 SALNGIVSELEAIGVKTDDEDK 1
S L GIV ELEAIGVK DDEDK
Sbjct: 129 SVLKGIVLELEAIGVKIDDEDK 150
>gb|PON70119.1| hypothetical protein PanWU01x14_082940 [Parasponia andersonii]
Length = 158
Score = 105 bits (263), Expect = 5e-26
Identities = 56/118 (47%), Positives = 76/118 (64%), Gaps = 11/118 (9%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQSK-----------HGLKGNISNMNKEKWEEVDLRAASAI 248
KFD INF LW+IQ+ D+LIQS+ G SN++ E +++D RA + I
Sbjct: 16 KFDRNINFGLWQIQIKDLLIQSRLHKILKGKEAYKGKNSEKSNISDEDKDDLDERAVNTI 75
Query: 247 HLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEKFHNLRMDEDMKVSDH 74
+ LAKN+L NV I +TKD W KL LYQAK I NR+ LKE+F+ LRM+ED K+S++
Sbjct: 76 RMFLAKNILINVLRITTTKDLWRKLGELYQAKRISNRVYLKEQFYTLRMNEDTKISEY 133
>gb|PHT69902.1| hypothetical protein T459_25006 [Capsicum annuum]
Length = 443
Score = 108 bits (271), Expect(2) = 5e-25
Identities = 60/129 (46%), Positives = 75/129 (58%), Gaps = 22/129 (17%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQS----------------------KHGLKGNISNMNKEKW 281
KFDGRINF LW++QV DVLIQS + S ++ E+W
Sbjct: 255 KFDGRINFGLWQVQVKDVLIQSGLHKALKERPTSGKGDKDSKKFESSEDSEKSRISDEEW 314
Query: 280 EEVDLRAASAIHLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEKFHNLRM 101
EE+D++ S I LCLAK VL NV + +TK WEKLE LYQ K I NRL LKE+FH L+M
Sbjct: 315 EELDMKVESQIRLCLAKYVLTNVIGLSTTKKLWEKLEELYQTKSISNRLYLKEQFHKLQM 374
Query: 100 DEDMKVSDH 74
E +SD+
Sbjct: 375 AEGTTISDY 383
Score = 33.1 bits (74), Expect(2) = 5e-25
Identities = 15/20 (75%), Positives = 18/20 (90%)
Frame = -2
Query: 66 SALNGIVSELEAIGVKTDDE 7
S LNGIVS+L++IGVK DDE
Sbjct: 385 SVLNGIVSKLKSIGVKIDDE 404
>ref|XP_016576563.1| PREDICTED: peptide methionine sulfoxide reductase-like [Capsicum
annuum]
Length = 413
Score = 103 bits (257), Expect(2) = 1e-24
Identities = 57/124 (45%), Positives = 75/124 (60%), Gaps = 17/124 (13%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQS-----------KHGLKGNISN------MNKEKWEEVDL 266
KFDGRINF LW++ + L ++ K K S ++ E+WEE+++
Sbjct: 9 KFDGRINFGLWQVLIQSGLHKALKERLTSEKDDKDSEKSESSKDLEKSRISDEEWEELNM 68
Query: 265 RAASAIHLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEKFHNLRMDEDMK 86
+A S I LCLAKNVLAN+ E+ +TK WEKLE LYQ K I NRL LKE+FH L+M E
Sbjct: 69 KAVSQIRLCLAKNVLANMIELSTTKKLWEKLEELYQTKSISNRLYLKEQFHKLQMAEGTT 128
Query: 85 VSDH 74
+ DH
Sbjct: 129 IYDH 132
Score = 37.4 bits (85), Expect(2) = 1e-24
Identities = 18/22 (81%), Positives = 19/22 (86%)
Frame = -2
Query: 66 SALNGIVSELEAIGVKTDDEDK 1
S LN IVSELE+IGVK DDEDK
Sbjct: 134 SVLNEIVSELESIGVKIDDEDK 155
>gb|PON43110.1| hypothetical protein PanWU01x14_276620 [Parasponia andersonii]
Length = 164
Score = 101 bits (251), Expect(2) = 1e-24
Identities = 47/75 (62%), Positives = 60/75 (80%)
Frame = -1
Query: 298 MNKEKWEEVDLRAASAIHLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEK 119
M+ E+W+++D RAASAI +CLAKNVLA V I++ K+ W KLE LYQAKG+ NR+ LKE
Sbjct: 1 MSDEEWDDLDERAASAIRMCLAKNVLARVVGIMTAKELWRKLEELYQAKGVSNRVYLKEL 60
Query: 118 FHNLRMDEDMKVSDH 74
FH LRM+E MK+SDH
Sbjct: 61 FHTLRMNEGMKISDH 75
Score = 39.3 bits (90), Expect(2) = 1e-24
Identities = 18/22 (81%), Positives = 20/22 (90%)
Frame = -2
Query: 66 SALNGIVSELEAIGVKTDDEDK 1
+ LNGIVSELEAIGVK +DEDK
Sbjct: 77 NVLNGIVSELEAIGVKVEDEDK 98
>gb|ESR55658.1| hypothetical protein CICLE_v10023471mg, partial [Citrus clementina]
Length = 185
Score = 100 bits (250), Expect(2) = 3e-24
Identities = 50/77 (64%), Positives = 59/77 (76%)
Frame = -1
Query: 304 SNMNKEKWEEVDLRAASAIHLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLK 125
++++ E WEE+D RAASAI LCLAKNVLANV I + K+ WEKLE LYQ K I NRL LK
Sbjct: 26 ASISDEDWEELDDRAASAIRLCLAKNVLANVGNIPTAKELWEKLEKLYQTKSISNRLYLK 85
Query: 124 EKFHNLRMDEDMKVSDH 74
E+FH LRM E K+SDH
Sbjct: 86 EQFHTLRMAEGTKISDH 102
Score = 38.9 bits (89), Expect(2) = 3e-24
Identities = 18/22 (81%), Positives = 20/22 (90%)
Frame = -2
Query: 66 SALNGIVSELEAIGVKTDDEDK 1
S LNGIVSELEAIGVK +D+DK
Sbjct: 104 SVLNGIVSELEAIGVKIEDDDK 125
>gb|PON56879.1| hypothetical protein TorRG33x02_294980 [Trema orientalis]
Length = 186
Score = 99.4 bits (246), Expect(2) = 3e-24
Identities = 47/75 (62%), Positives = 58/75 (77%)
Frame = -1
Query: 298 MNKEKWEEVDLRAASAIHLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEK 119
M+ W+++D RAASAI +CLAKNVLANV I + KD W KLE LYQAKG+ NR+ LKE+
Sbjct: 1 MSDVDWDDLDERAASAIRMCLAKNVLANVLGITTAKDLWRKLEELYQAKGVSNRVYLKEQ 60
Query: 118 FHNLRMDEDMKVSDH 74
FH LRM+E K+SDH
Sbjct: 61 FHTLRMNEGTKISDH 75
Score = 40.0 bits (92), Expect(2) = 3e-24
Identities = 19/22 (86%), Positives = 20/22 (90%)
Frame = -2
Query: 66 SALNGIVSELEAIGVKTDDEDK 1
S LNGIVSELEAIGVK +DEDK
Sbjct: 77 SVLNGIVSELEAIGVKIEDEDK 98
>gb|PON66942.1| Pol-like polyprotein/retrotransposon [Trema orientalis]
Length = 120
Score = 98.2 bits (243), Expect = 2e-23
Identities = 54/96 (56%), Positives = 64/96 (66%), Gaps = 11/96 (11%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQS--------KHGLKGNIS---NMNKEKWEEVDLRAASAI 248
KFDG INF LW+IQV D+LIQS + LKG S +M+ E WE++D RAASAI
Sbjct: 16 KFDGNINFGLWQIQVKDLLIQSGLHKALKGREALKGRDSEKSSMSDEDWEDLDERAASAI 75
Query: 247 HLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*N 140
+CLAKNVLANV I + KD W KL LYQA G+ N
Sbjct: 76 RICLAKNVLANVLRITTAKDLWGKLGELYQANGVSN 111
>ref|XP_013443246.1| hypothetical protein MTR_0027s0030 [Medicago truncatula]
gb|KEH17271.1| hypothetical protein MTR_0027s0030 [Medicago truncatula]
Length = 99
Score = 95.9 bits (237), Expect = 8e-23
Identities = 47/76 (61%), Positives = 58/76 (76%)
Frame = -1
Query: 337 IQSKHGLKGNISNMNKEKWEEVDLRAASAIHLCLAKNVLANVQEILSTKDFWEKLEGLYQ 158
+Q+ H LKGNIS M+ EKWEE+DLRA S I + LAKN+L NV STK WEKL+ LY+
Sbjct: 24 VQASHVLKGNISKMDNEKWEELDLRALSTICMSLAKNILVNVLGTSSTKVLWEKLDELYK 83
Query: 157 AKGI*NRLLLKEKFHN 110
AK + N+LLLKEKFH+
Sbjct: 84 AKHVSNQLLLKEKFHS 99
>gb|PNX62013.1| F-box/kelch-repeat protein, partial [Trifolium pratense]
Length = 75
Score = 94.0 bits (232), Expect = 2e-22
Identities = 48/63 (76%), Positives = 52/63 (82%), Gaps = 2/63 (3%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQS--KHGLKGNISNMNKEKWEEVDLRAASAIHLCLAKNVL 221
KFDGRINF LWK+QV+DVLIQS LKGN S M +KWEE+DLRAASAI LCLAKNVL
Sbjct: 13 KFDGRINFGLWKVQVSDVLIQSGLHKELKGNTSKMEADKWEELDLRAASAIRLCLAKNVL 72
Query: 220 ANV 212
ANV
Sbjct: 73 ANV 75
>gb|PON68556.1| hypothetical protein PanWU01x14_095270 [Parasponia andersonii]
Length = 127
Score = 95.5 bits (236), Expect = 2e-22
Identities = 43/75 (57%), Positives = 57/75 (76%)
Frame = -1
Query: 298 MNKEKWEEVDLRAASAIHLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEK 119
M+ E+W+++D RA + I +CLAKNVLANV I++ KD W K E LYQAKG+ NR+ LKE+
Sbjct: 1 MSDEEWDDMDERAVTTIRMCLAKNVLANVLGIITAKDLWRKFEELYQAKGVSNRVYLKEQ 60
Query: 118 FHNLRMDEDMKVSDH 74
FH L M+E K+SDH
Sbjct: 61 FHTLYMNEGTKISDH 75
>ref|XP_018827443.1| PREDICTED: uncharacterized protein LOC108996131 [Juglans regia]
Length = 166
Score = 92.8 bits (229), Expect = 8e-21
Identities = 44/72 (61%), Positives = 55/72 (76%)
Frame = -1
Query: 304 SNMNKEKWEEVDLRAASAIHLCLAKNVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLK 125
S M+ E WE++DLRAASAI LCLAKNVLAN+ I + K+ WEKLE LYQ KG+ NR+ LK
Sbjct: 31 SVMSDEDWEDLDLRAASAIRLCLAKNVLANIHGISTAKELWEKLEELYQTKGVSNRVYLK 90
Query: 124 EKFHNLRMDEDM 89
E FH L + E++
Sbjct: 91 ELFHTLHISEEV 102
>gb|KJB48829.1| hypothetical protein B456_008G089200 [Gossypium raimondii]
Length = 164
Score = 90.1 bits (222), Expect = 8e-20
Identities = 48/112 (42%), Positives = 69/112 (61%), Gaps = 5/112 (4%)
Frame = -1
Query: 394 KFDGRINFDLWKIQVNDVLIQSKHGLKGNIS-----NMNKEKWEEVDLRAASAIHLCLAK 230
KFDG NF+LW++++ +L+QS GLK ++ N+NK KWEE+D +A S I LCLA
Sbjct: 10 KFDGETNFNLWQVRMMAILVQS--GLKKVVTRKKPENLNKTKWEELDGKALSVIQLCLAN 67
Query: 229 NVLANVQEILSTKDFWEKLEGLYQAKGI*NRLLLKEKFHNLRMDEDMKVSDH 74
VL V ++ W++LE LY K + NRL+LK+ RM+E + DH
Sbjct: 68 TVLQEVLMEKTSSALWKRLETLYATKSLANRLVLKQHLFTFRMNEGEILRDH 119
>gb|KGN63177.1| hypothetical protein Csa_2G406750 [Cucumis sativus]
Length = 317
Score = 93.2 bits (230), Expect = 1e-19
Identities = 50/85 (58%), Positives = 62/85 (72%), Gaps = 4/85 (4%)
Frame = -1
Query: 394 KFDGRINFDLWKIQ-VNDVLIQS--KHGLKGNI-SNMNKEKWEEVDLRAASAIHLCLAKN 227
KFDGRINF LW++Q V DVLIQ G+KG +NM+ E+WEE+DLRA S I L LAKN
Sbjct: 211 KFDGRINFGLWQLQAVKDVLIQVGLHKGVKGRQDTNMSDEEWEEMDLRAISVIRLNLAKN 270
Query: 226 VLANVQEILSTKDFWEKLEGLYQAK 152
++ANV I + K WEKLE +YQA+
Sbjct: 271 IIANVDRISTAKQLWEKLEAMYQAR 295