BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00027335
         (648 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PNY15602.1| ribonuclease H [Trifolium pratense]                     80   4e-26
gb|PNX66369.1| ribonuclease H, partial [Trifolium pratense]            76   9e-24
dbj|GAU14934.1| hypothetical protein TSUD_47270 [Trifolium subte...    69   1e-23
gb|PNX81608.1| ribonuclease H [Trifolium pratense]                     69   9e-23
dbj|GAU38380.1| hypothetical protein TSUD_147480 [Trifolium subt...    71   3e-22
gb|PNY03121.1| ribonuclease H, partial [Trifolium pratense]            68   4e-22
dbj|GAU38338.1| hypothetical protein TSUD_61990 [Trifolium subte...    72   6e-22
gb|PNY15498.1| ribonuclease H [Trifolium pratense]                     67   6e-22
dbj|GAU44140.1| hypothetical protein TSUD_188010 [Trifolium subt...    69   8e-22
dbj|GAU32702.1| hypothetical protein TSUD_145760 [Trifolium subt...    65   2e-21
gb|PNY12420.1| ribonuclease H [Trifolium pratense]                     68   3e-21
gb|PNX95557.1| ribonuclease H [Trifolium pratense]                     65   8e-21
dbj|GAU30604.1| hypothetical protein TSUD_392950 [Trifolium subt...    67   1e-20
dbj|GAU43502.1| hypothetical protein TSUD_398950 [Trifolium subt...    65   2e-20
gb|PNY10193.1| ribonuclease H [Trifolium pratense]                     66   2e-20
dbj|GAU31911.1| hypothetical protein TSUD_270960 [Trifolium subt...    66   2e-20
gb|PNX91284.1| ribonuclease H [Trifolium pratense]                     74   3e-20
ref|XP_023908176.1| uncharacterized protein LOC112019864 [Quercu...    82   4e-20
gb|PNX97917.1| ribonuclease H [Trifolium pratense]                     64   8e-20
gb|PNX75059.1| ribonuclease H [Trifolium pratense]                     64   1e-19

>gb|PNY15602.1| ribonuclease H [Trifolium pratense]
          Length = 437

 Score = 79.7 bits (195), Expect(2) = 4e-26
 Identities = 36/64 (56%), Positives = 43/64 (67%)
 Frame = +2

Query: 74  GVSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAE 253
           GV  I W+ P +GWV LNTDG+     G   CGG++RDH G WC GFAK +G  SVL AE
Sbjct: 264 GVCWIGWKVPEEGWVKLNTDGASK-GEGLSGCGGIIRDHQGNWCGGFAKFVGTGSVLIAE 322

Query: 254 LWGL 265
           LWG+
Sbjct: 323 LWGV 326



 Score = 66.6 bits (161), Expect(2) = 4e-26
 Identities = 35/94 (37%), Positives = 54/94 (57%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G++ GL+   ++  +++ + +DS      I     ++    SLV  I RLL   W+VK+ 
Sbjct: 325 GVLEGLKLVWRKGYRKVEVNIDSISVVKMILNGMTSSALGFSLVKSIGRLLDEQWEVKIS 384

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPSSV 540
           H  RE N+CAD LAS+G     ++VFFE CPSS+
Sbjct: 385 HSYRETNKCADALASMGCILDCNIVFFETCPSSI 418


>gb|PNX66369.1| ribonuclease H, partial [Trifolium pratense]
          Length = 252

 Score = 75.9 bits (185), Expect(2) = 9e-24
 Identities = 34/63 (53%), Positives = 45/63 (71%)
 Frame = +2

Query: 77  VSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAEL 256
           V +I+W+PP +GWV LNTDG+    S    CGG++RD +G W  GFAK++GICS   AEL
Sbjct: 80  VVMINWKPPSEGWVKLNTDGAYKEGS-VAGCGGVIRDSNGVWRGGFAKNLGICSAYVAEL 138

Query: 257 WGL 265
           WG+
Sbjct: 139 WGV 141



 Score = 62.8 bits (151), Expect(2) = 9e-24
 Identities = 31/92 (33%), Positives = 51/92 (55%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G++ GL++       ++ L VDS V  + +           +LV+ IQR+L L W+V + 
Sbjct: 140 GVLEGLRYANSLGFNRVELNVDSSVVIHVLRRPGYGRPLGGALVMRIQRMLDLDWEVVIN 199

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPS 534
           H  RE N+CAD LA++G      +V++E CP+
Sbjct: 200 HSYREANKCADVLANIGCAIDTHMVYYETCPT 231


>dbj|GAU14934.1| hypothetical protein TSUD_47270 [Trifolium subterraneum]
          Length = 650

 Score = 69.3 bits (168), Expect(2) = 1e-23
 Identities = 36/94 (38%), Positives = 54/94 (57%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G++ GL+    R  ++I + VDS      I     ++    SL+  I+RLL   W+VK+ 
Sbjct: 538 GVLEGLKLAKGRRFRKIEVNVDSSSVVKMIMNGESSSSMGFSLIKSIRRLLDGEWEVKIS 597

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPSSV 540
           H  RE N+CAD LAS+G     ++VFFE CPS++
Sbjct: 598 HTYREANKCADALASMGCTLDCNIVFFETCPSNI 631



 Score = 68.9 bits (167), Expect(2) = 1e-23
 Identities = 29/60 (48%), Positives = 39/60 (65%)
 Frame = +2

Query: 86  ISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAELWGL 265
           I W+ P +GWV +NTDG+     G   CGG++ DH G WC GFAK +G  + + AELWG+
Sbjct: 481 IGWKVPEEGWVKVNTDGASK-GEGLAGCGGIIIDHRGSWCGGFAKYVGTGNAIIAELWGV 539


>gb|PNX81608.1| ribonuclease H [Trifolium pratense]
          Length = 630

 Score = 68.6 bits (166), Expect(2) = 9e-23
 Identities = 30/62 (48%), Positives = 44/62 (70%), Gaps = 2/62 (3%)
 Frame = +2

Query: 86  ISWEPPLDGWVCLNTDGSV--HLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAELW 259
           ++W+ P DGW+ LNTDG+   H ++G   CGGLLR+  GQW  GF++++G C+   AELW
Sbjct: 461 VTWQCPEDGWLSLNTDGASRGHTSAG---CGGLLRNSEGQWLGGFSRNLGRCNAYIAELW 517

Query: 260 GL 265
           G+
Sbjct: 518 GV 519



 Score = 66.6 bits (161), Expect(2) = 9e-23
 Identities = 34/92 (36%), Positives = 51/92 (55%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G+  GL     +  K++ + VDS V  + +      +V    L+ EI+RLLAL W++K+ 
Sbjct: 518 GVHDGLCLARDKGAKKLKVYVDSSVVVHTLNSTTGGSVVGWRLIQEIRRLLALDWEIKVC 577

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPS 534
           H  RE N CAD LA++G      +  +EQCPS
Sbjct: 578 HSYREANACADALANMGCDHGPGIRVYEQCPS 609


>dbj|GAU38380.1| hypothetical protein TSUD_147480 [Trifolium subterraneum]
          Length = 194

 Score = 70.9 bits (172), Expect(2) = 3e-22
 Identities = 33/63 (52%), Positives = 43/63 (68%)
 Frame = +2

Query: 77  VSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAEL 256
           VS I W+P  DGWV LNT G+    S   +CGG+LR++HG+W  GF+K +G CS   AEL
Sbjct: 34  VSYIGWKPHDDGWVKLNTYGACRERS-VAACGGVLRNNHGEWIRGFSKFLGTCSAYVAEL 92

Query: 257 WGL 265
           WG+
Sbjct: 93  WGV 95



 Score = 62.4 bits (150), Expect(2) = 3e-22
 Identities = 33/88 (37%), Positives = 50/88 (56%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G++ GL++      +++ L VDS      I     A+    SLV EI RL++  W+VK+ 
Sbjct: 94  GVLEGLKYNWSLGFRKVELNVDSVAVAKVIKEGGTASNMGYSLVKEIHRLVSFEWEVKIF 153

Query: 439 HILREGNRCADELASLGNGPPGSLVFFE 522
           H  RE NRCAD LA++G     ++VFF+
Sbjct: 154 HSYRETNRCADTLANMGYSLEENIVFFD 181


>gb|PNY03121.1| ribonuclease H, partial [Trifolium pratense]
          Length = 952

 Score = 68.2 bits (165), Expect(2) = 4e-22
 Identities = 32/92 (34%), Positives = 54/92 (58%)
 Frame = +1

Query: 259  GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
            G++ GL++  +    ++ L +DSE     +  R  ++    +LV +I RLL + W+V++ 
Sbjct: 861  GVLEGLRYVHRMGLVKVELNIDSEAVVQVVKNRXMSSSLGVALVKQIWRLLDMNWEVEVS 920

Query: 439  HILREGNRCADELASLGNGPPGSLVFFEQCPS 534
            H  RE N+CAD LA+LG      +VF++ CPS
Sbjct: 921  HTYREANKCADALANLGCSLANEIVFYDSCPS 952



 Score = 64.7 bits (156), Expect(2) = 4e-22
 Identities = 29/63 (46%), Positives = 39/63 (61%)
 Frame = +2

Query: 77  VSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAEL 256
           V  I W PP   +V LNTDG+ +  +    CGG++R   G+W  GFAK +G+CS   AEL
Sbjct: 801 VRWIGWRPPKPNFVKLNTDGA-YKENKAAGCGGVIRGSEGEWMGGFAKGVGLCSAFVAEL 859

Query: 257 WGL 265
           WG+
Sbjct: 860 WGV 862


>dbj|GAU38338.1| hypothetical protein TSUD_61990 [Trifolium subterraneum]
          Length = 813

 Score = 71.6 bits (174), Expect(2) = 6e-22
 Identities = 32/63 (50%), Positives = 43/63 (68%)
 Frame = +2

Query: 77  VSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAEL 256
           +S+I W PP D +V LNTDG+ +  +    CGG+ R +HG+W  GFAK +GICSV  AE 
Sbjct: 641 ISMIRWNPPSDLFVKLNTDGA-YKENAIVGCGGVTRGNHGEWLGGFAKCVGICSVFVAES 699

Query: 257 WGL 265
           WG+
Sbjct: 700 WGV 702



 Score = 60.8 bits (146), Expect(2) = 6e-22
 Identities = 32/94 (34%), Positives = 49/94 (52%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G+  GL +  +   ++++L +DSEV    I   +  + A  SL+ +I RLL + W V++ 
Sbjct: 701 GVFEGLSYVHRLGFRKVVLHIDSEVVVRVIKNGSSDSSAGSSLLTQIWRLLEMDWIVEVS 760

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPSSV 540
           H  RE N CAD LA+LG       V F   P  +
Sbjct: 761 HTYREANNCADALANLGCSLDYDTVIFNDFPPQI 794


>gb|PNY15498.1| ribonuclease H [Trifolium pratense]
          Length = 378

 Score = 67.0 bits (162), Expect(2) = 6e-22
 Identities = 37/94 (39%), Positives = 52/94 (55%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G+  GL+    R  ++I L VDS    + I      NV +  +V  IQ++L+L W+VKL 
Sbjct: 266 GVSEGLKLARDRGFQRIELCVDSVSVISIIQNGGGGNVMAHRIVQSIQQMLSLNWEVKLK 325

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPSSV 540
           HI RE N CAD LA+L    P  +V F+ CP  +
Sbjct: 326 HIYREENHCADGLANLAFILPKGIVLFDVCPDGI 359



 Score = 65.5 bits (158), Expect(2) = 6e-22
 Identities = 29/60 (48%), Positives = 40/60 (66%)
 Frame = +2

Query: 86  ISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAELWGL 265
           + W+PP  GWV +NTDG+   + G  SCGGL+R    +W  GF+K IG C+ + AELWG+
Sbjct: 209 VKWQPPSIGWVKVNTDGASK-SDGSASCGGLIRGSDCEWLGGFSKHIGRCTSVVAELWGV 267


>dbj|GAU44140.1| hypothetical protein TSUD_188010 [Trifolium subterraneum]
          Length = 200

 Score = 68.9 bits (167), Expect(2) = 8e-22
 Identities = 31/60 (51%), Positives = 40/60 (66%)
 Frame = +2

Query: 86  ISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAELWGL 265
           I W+PP D +V LNTDG+    +    CGG++R +HG+W  GFAK IG CS   AELWG+
Sbjct: 53  IGWKPPSDNFVRLNTDGA-RKDNNKAECGGIIRGNHGEWLGGFAKVIGECSAFIAELWGV 111



 Score = 63.2 bits (152), Expect(2) = 8e-22
 Identities = 33/91 (36%), Positives = 50/91 (54%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G+  GL    +   +++ + +DS V    I      N    SLVL I++LL L W+V + 
Sbjct: 110 GVFEGLTLARRMGFRKVEVHIDSVVVVQVITTGKLHNKIGWSLVLNIRKLLELDWEVIIA 169

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCP 531
           H  RE N+CAD LA++G     +L+F+E CP
Sbjct: 170 HAYRETNKCADALANVGCQLNRTLIFYEDCP 200


>dbj|GAU32702.1| hypothetical protein TSUD_145760 [Trifolium subterraneum]
          Length = 739

 Score = 65.5 bits (158), Expect(2) = 2e-21
 Identities = 28/62 (45%), Positives = 40/62 (64%), Gaps = 2/62 (3%)
 Frame = +2

Query: 86  ISWEPPLDGWVCLNTDGSV--HLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAELW 259
           I W+PP+ GW+ LNTDG+    + +G   CGG++R+  G W  GFA  +G+CS    ELW
Sbjct: 570 IGWKPPVIGWIKLNTDGACKGRITTG---CGGVIRNSSGVWIRGFATHVGVCSAFTVELW 626

Query: 260 GL 265
           G+
Sbjct: 627 GV 628



 Score = 65.5 bits (158), Expect(2) = 2e-21
 Identities = 34/94 (36%), Positives = 51/94 (54%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G++ GL++   R  + + L VDS      I      +V   SL+  I RLL   W+VK+ 
Sbjct: 627 GVLEGLRYAWYRGFRFVELDVDSVAVVQAIKSGVATSVIGISLLRSICRLLEEEWEVKIA 686

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPSSV 540
           H  RE N CAD LA++G     ++V+F++CP  V
Sbjct: 687 HSYREANSCADALANIGCSLHLNIVYFDECPDQV 720


>gb|PNY12420.1| ribonuclease H [Trifolium pratense]
          Length = 1341

 Score = 68.2 bits (165), Expect(2) = 3e-21
 Identities = 32/94 (34%), Positives = 53/94 (56%)
 Frame = +1

Query: 259  GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
            G++ GL++        + L +DSE     +  R   + +  +LV +I R+L + W V++ 
Sbjct: 1229 GVLEGLRYVHHIGFTMVELNIDSEAVVKVVKARQLGSSSGAALVKQIWRMLDMNWKVEIS 1288

Query: 439  HILREGNRCADELASLGNGPPGSLVFFEQCPSSV 540
            H  RE N+CAD LA+LG+     L+FF+ CPS +
Sbjct: 1289 HTYREANKCADALANLGSTLDKELIFFDDCPSHI 1322



 Score = 62.0 bits (149), Expect(2) = 3e-21
 Identities = 27/69 (39%), Positives = 42/69 (60%)
 Frame = +2

Query: 59   VDGSAGVSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICS 238
            ++ +  +  I W PP   +V LNTDG+ +  +    CGG++R   G+W  G+AK +G+CS
Sbjct: 1163 LERTRSIRWIGWSPPKMNFVKLNTDGA-YKENRAAGCGGVIRGCEGEWLGGYAKGVGLCS 1221

Query: 239  VLDAELWGL 265
               AELWG+
Sbjct: 1222 AFVAELWGV 1230


>gb|PNX95557.1| ribonuclease H [Trifolium pratense]
          Length = 321

 Score = 65.5 bits (158), Expect(2) = 8e-21
 Identities = 29/95 (30%), Positives = 52/95 (54%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G++ GL    +     + L +DS+   + +           ++V +I+RLL + W+VK++
Sbjct: 209 GVLEGLLLAKRLGFSTVELSIDSKAVVHVVISGRTQGADGYAIVKKIRRLLKMDWNVKIL 268

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPSSVG 543
           H  RE N+CAD LA++G      ++F+E CP  +G
Sbjct: 269 HEYREANKCADALANIGCNLDHEVIFYEACPMEIG 303



 Score = 63.2 bits (152), Expect(2) = 8e-21
 Identities = 29/63 (46%), Positives = 39/63 (61%)
 Frame = +2

Query: 77  VSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAEL 256
           +S I W+ P+  +V LNTDGS         CGG++R + G+W  GFAK +G CS   AEL
Sbjct: 149 LSHIQWQQPVGNYVKLNTDGSCKEGKR-AGCGGVIRGNQGEWLGGFAKGVGQCSAFVAEL 207

Query: 257 WGL 265
           WG+
Sbjct: 208 WGV 210


>dbj|GAU30604.1| hypothetical protein TSUD_392950 [Trifolium subterraneum]
          Length = 233

 Score = 67.4 bits (163), Expect(2) = 1e-20
 Identities = 28/60 (46%), Positives = 36/60 (60%)
 Frame = +2

Query: 86  ISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAELWGL 265
           + W P   GW+CLNTD +    +  + CGGL RD  G W  GF+KS+G  +   AELWGL
Sbjct: 63  VRWYPSEHGWICLNTDDAFKSHNTKDGCGGLFRDEDGHWIRGFSKSLGSATAYVAELWGL 122



 Score = 60.8 bits (146), Expect(2) = 1e-20
 Identities = 28/77 (36%), Positives = 46/77 (59%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G++ G+         ++ +Q+DSE+  + I      NV+  S++ +I+ LL+L W VK+ 
Sbjct: 121 GLLEGISIARSMGFNKLEVQMDSEIIVSIINKHGHGNVSGWSIIKKIRSLLSLDWSVKIC 180

Query: 439 HILREGNRCADELASLG 489
           H  RE NRCAD LA++G
Sbjct: 181 HFYREANRCADMLANMG 197


>dbj|GAU43502.1| hypothetical protein TSUD_398950 [Trifolium subterraneum]
          Length = 1962

 Score = 65.1 bits (157), Expect(2) = 2e-20
 Identities = 29/63 (46%), Positives = 39/63 (61%)
 Frame = +2

Query: 77   VSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAEL 256
            ++ I W+PP   WV LNTDG+         CGG++R + G+W  GFAK +G CS   AEL
Sbjct: 1790 IAHIRWKPPEGVWVKLNTDGACK-EDNRAGCGGVVRGNQGEWLGGFAKGVGKCSAFVAEL 1848

Query: 257  WGL 265
            WG+
Sbjct: 1849 WGV 1851



 Score = 62.4 bits (150), Expect(2) = 2e-20
 Identities = 27/94 (28%), Positives = 53/94 (56%)
 Frame = +1

Query: 259  GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
            G++ GL    +     + L +DS++  + +      +V   ++V +++RLL L W+V++ 
Sbjct: 1850 GVLEGLLLVQRMGFANVELSIDSKIVVHALTSGTATSVDGYAIVRKVRRLLLLDWNVRVT 1909

Query: 439  HILREGNRCADELASLGNGPPGSLVFFEQCPSSV 540
            H  RE N+CAD LA++G        +F++CP+ +
Sbjct: 1910 HEYREANKCADALANIGCTLDMECTYFQECPAEI 1943


>gb|PNY10193.1| ribonuclease H [Trifolium pratense]
          Length = 221

 Score = 66.2 bits (160), Expect(2) = 2e-20
 Identities = 30/61 (49%), Positives = 39/61 (63%)
 Frame = +2

Query: 83  LISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAELWG 262
           LI W+PP  GWV LN+DG+     G   CG ++R   G+W  GFAK++GICS   AE WG
Sbjct: 54  LIGWKPPPVGWVKLNSDGACK-HDGSAGCGRIIRGSDGEWLGGFAKNLGICSAYVAEQWG 112

Query: 263 L 265
           +
Sbjct: 113 V 113



 Score = 61.2 bits (147), Expect(2) = 2e-20
 Identities = 33/92 (35%), Positives = 49/92 (53%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G+  G +   +   K + L VDS +  N +        +SR+LV +I+ L+A+ W+V + 
Sbjct: 112 GVYAGFRLARRLGFKAVELNVDSLLVANVLNSN---QCSSRALVAKIRSLIAMDWEVVVK 168

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPS 534
           H  RE NRCAD LAS G        F+E CP+
Sbjct: 169 HSYREANRCADALASYGATLEDGFCFYETCPA 200


>dbj|GAU31911.1| hypothetical protein TSUD_270960 [Trifolium subterraneum]
          Length = 853

 Score = 66.2 bits (160), Expect(2) = 2e-20
 Identities = 29/65 (44%), Positives = 43/65 (66%), Gaps = 2/65 (3%)
 Frame = +2

Query: 77  VSLISWEPPLDGWVCLNTDGSV--HLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDA 250
           +S+I W PP   +V LNTDG+   H+ +G   CGG++R   G+W  GFAK +G+C+   A
Sbjct: 696 ISMIRWSPPKANFVKLNTDGACKEHIIAG---CGGIVRGSEGEWIGGFAKCVGMCNAFIA 752

Query: 251 ELWGL 265
           E+WG+
Sbjct: 753 EMWGV 757



 Score = 60.8 bits (146), Expect(2) = 2e-20
 Identities = 31/95 (32%), Positives = 57/95 (60%), Gaps = 1/95 (1%)
 Frame = +1

Query: 259  GIIVGLQFCLQRNCKQIILQVDSEVACNEI-FGRARANVASRSLVLEIQRLLALPWDVKL 435
            G++ GL++  +   +++ L +DS      I  GR +++  S +L  +I +L+A+ W+V++
Sbjct: 756  GVLEGLRYVRRLGFRKVELNIDSAAVVQVIKTGRLQSSTGS-ALARQIWKLMAMDWEVEV 814

Query: 436  VHILREGNRCADELASLGNGPPGSLVFFEQCPSSV 540
             HI RE N+CAD LA++G+     +  F  CPS +
Sbjct: 815  NHIYREANKCADALANMGSNFDYDIKIFYSCPSQL 849


>gb|PNX91284.1| ribonuclease H [Trifolium pratense]
          Length = 178

 Score = 73.9 bits (180), Expect(2) = 3e-20
 Identities = 30/63 (47%), Positives = 41/63 (65%)
 Frame = +2

Query: 77  VSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAEL 256
           ++ ISW+ P  GW CLNTDG+     G   CGGL+ D +G+W  GF++++GI S   AEL
Sbjct: 9   ITNISWKAPTHGWYCLNTDGAAKGIDGLTGCGGLVHDENGRWIHGFSRNLGIASAYMAEL 68

Query: 257 WGL 265
           W L
Sbjct: 69  WSL 71



 Score = 52.8 bits (125), Expect(2) = 3e-20
 Identities = 27/71 (38%), Positives = 43/71 (60%), Gaps = 2/71 (2%)
 Frame = +1

Query: 283 CLQRN--CKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLVHILREG 456
           CL R    + + +Q+DS+V    +      + A  SL+ +I+ LL   W+VK++H+ RE 
Sbjct: 76  CLAREYXVRHLEVQLDSKVVVCSLQEGKLGSAAGWSLIKKIKELLNYSWNVKIIHVYREA 135

Query: 457 NRCADELASLG 489
           NRCAD LA++G
Sbjct: 136 NRCADILANIG 146


>ref|XP_023908176.1| uncharacterized protein LOC112019864 [Quercus suber]
          Length = 1638

 Score = 82.4 bits (202), Expect(2) = 4e-20
 Identities = 38/72 (52%), Positives = 46/72 (63%)
 Frame = +2

Query: 50   SSDVDGSAGVSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIG 229
            S   + + G+  I W  P DGW  LNTDGS++ A+G   CGGL+RD  GQW  GFAK I 
Sbjct: 1449 SQPEEKNMGLKEIRWTKPADGWYKLNTDGSINSANGLAGCGGLIRDKDGQWVAGFAKKID 1508

Query: 230  ICSVLDAELWGL 265
            + S L AELWGL
Sbjct: 1509 VSSSLAAELWGL 1520



 Score = 43.9 bits (102), Expect(2) = 4e-20
 Identities = 26/77 (33%), Positives = 41/77 (53%)
 Frame = +1

Query: 259  GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
            G+  GL  C+  + + + +++D+  A + I   +R+N     LV + + LL      K+V
Sbjct: 1519 GLREGLALCVDVHAQAVEVELDAAAAISLISCNSRSNGDFSGLVDDCRDLLLQLPQAKVV 1578

Query: 439  HILREGNRCADELASLG 489
            H  RE N CAD LA LG
Sbjct: 1579 HCYREANFCADALAKLG 1595


>gb|PNX97917.1| ribonuclease H [Trifolium pratense]
          Length = 712

 Score = 63.5 bits (153), Expect(2) = 8e-20
 Identities = 30/63 (47%), Positives = 40/63 (63%)
 Frame = +2

Query: 77  VSLISWEPPLDGWVCLNTDGSVHLASGYESCGGLLRDHHGQWCWGFAKSIGICSVLDAEL 256
           V+ I W+PP   +V LNTDG+   A     CGG++R + G+W  GFAK +G CS   AEL
Sbjct: 540 VAHIHWKPPEGVFVKLNTDGASK-AGNRAGCGGVIRGNQGEWLGGFAKGVGNCSAFVAEL 598

Query: 257 WGL 265
           WG+
Sbjct: 599 WGV 601



 Score = 61.6 bits (148), Expect(2) = 8e-20
 Identities = 29/94 (30%), Positives = 53/94 (56%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G++ GL    +   + + L +DS+   + I      +    ++V +I+RLL + W+VK++
Sbjct: 600 GVLEGLLLVQRMGFENVELSIDSKAVVHVITAGKATSADGYAIVRKIRRLLLMDWNVKVL 659

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPSSV 540
           H  RE N+CAD LA+ G      L+F+++CP  +
Sbjct: 660 HEYREANKCADALANTGCILDLELIFYQECPMEI 693


>gb|PNX75059.1| ribonuclease H [Trifolium pratense]
          Length = 362

 Score = 64.3 bits (155), Expect(2) = 1e-19
 Identities = 35/94 (37%), Positives = 49/94 (52%)
 Frame = +1

Query: 259 GIIVGLQFCLQRNCKQIILQVDSEVACNEIFGRARANVASRSLVLEIQRLLALPWDVKLV 438
           G+  GL         QI + VDS V    +      +V    LV EI+RLLAL W++K+ 
Sbjct: 250 GVYDGLCLARSLGATQIKVHVDSSVVVQTLNSTNGGSVVGWRLVQEIRRLLALDWEIKVC 309

Query: 439 HILREGNRCADELASLGNGPPGSLVFFEQCPSSV 540
           H  RE N CAD LA++G      L  ++ CP+S+
Sbjct: 310 HCYREANACADALANMGCEHGPGLRIYDNCPTSL 343



 Score = 60.1 bits (144), Expect(2) = 1e-19
 Identities = 29/63 (46%), Positives = 43/63 (68%), Gaps = 2/63 (3%)
 Frame = +2

Query: 83  LISWEPPLDGWVCLNTDGSVHLASG--YESCGGLLRDHHGQWCWGFAKSIGICSVLDAEL 256
           +I+W+ P +GW+ LNTDG+   +SG     CGGL+R+  GQ   GF++++G CS   AEL
Sbjct: 192 VIAWQRPENGWLSLNTDGA---SSGDVTAGCGGLIRNSVGQSLGGFSRNLGCCSAYLAEL 248

Query: 257 WGL 265
           WG+
Sbjct: 249 WGV 251