BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00022918
         (350 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PNY14125.1| nuclear-interacting partner of ALK, partial [Trif...   137   2e-35
dbj|GAU19032.1| hypothetical protein TSUD_193700 [Trifolium subt...   134   3e-35
dbj|GAU39112.1| hypothetical protein TSUD_320870, partial [Trifo...   131   2e-33
ref|XP_004508585.1| PREDICTED: uncharacterized protein LOC101510...   125   3e-31
ref|XP_004508584.1| PREDICTED: uncharacterized protein LOC101510...   125   3e-31
ref|XP_020226540.1| uncharacterized protein LOC109808107 [Cajanu...   122   8e-30
ref|XP_003609078.2| C3HC zinc finger-like protein [Medicago trun...   119   7e-29
ref|XP_003550849.1| PREDICTED: uncharacterized protein LOC100804...   117   4e-28
ref|XP_006600786.1| PREDICTED: uncharacterized protein LOC100804...   112   2e-26
ref|XP_006579725.1| PREDICTED: uncharacterized protein LOC100799...   112   2e-26
gb|KHN15160.1| Nuclear-interacting partner of ALK [Glycine soja]      112   3e-26
ref|XP_003525642.1| PREDICTED: uncharacterized protein LOC100799...   112   3e-26
ref|XP_019439275.1| PREDICTED: uncharacterized protein LOC109344...   111   5e-26
ref|XP_019439284.1| PREDICTED: uncharacterized protein LOC109344...    87   1e-17
ref|XP_007155162.1| hypothetical protein PHAVU_003G178600g [Phas...    82   9e-16
ref|XP_014509169.1| uncharacterized protein LOC106768499 [Vigna ...    82   1e-15
dbj|BAT76464.1| hypothetical protein VIGAN_01447200 [Vigna angul...    78   3e-14
ref|XP_017420306.1| PREDICTED: uncharacterized protein LOC108330...    78   3e-14
ref|XP_016188990.1| uncharacterized protein LOC107630396 [Arachi...    59   2e-07

>gb|PNY14125.1| nuclear-interacting partner of ALK, partial [Trifolium pratense]
          Length = 652

 Score =  137 bits (346), Expect = 2e-35
 Identities = 78/117 (66%), Positives = 90/117 (76%), Gaps = 1/117 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVPVSSE+RE SN ET  QA I DS V NV+EGTHSA QPS LKDKMPV AE+D  KSSS
Sbjct: 442 LVPVSSEIREISNCETDSQASIRDSVVDNVLEGTHSAQQPSSLKDKMPVQAETDRSKSSS 501

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSD-VHSSLGKFR 348
           VG PS S+K+LA D ALSVSH+   K+ SNV + GVKER  NP +S+ VH+SLGKF+
Sbjct: 502 VGDPSSSKKNLAEDEALSVSHK--TKEASNVETCGVKERVENPTSSEGVHNSLGKFK 556


>dbj|GAU19032.1| hypothetical protein TSUD_193700 [Trifolium subterraneum]
          Length = 448

 Score =  134 bits (338), Expect = 3e-35
 Identities = 74/117 (63%), Positives = 89/117 (76%), Gaps = 1/117 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVP+SSE+R+ SN ET  QA IHDS V NV+EGTHSA QPS  KDKMPV AE+D +KSSS
Sbjct: 238 LVPLSSEIRDISNCETDSQASIHDSVVDNVLEGTHSAQQPSSFKDKMPVQAETDRLKSSS 297

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSD-VHSSLGKFR 348
           VG PS SQK+LA   ALS SH+   K+ SNV + G+KER  NP +S+ VH+SLGKF+
Sbjct: 298 VGDPSSSQKNLAEGEALSASHK--TKEASNVETCGLKERVENPTSSEVVHNSLGKFK 352


>dbj|GAU39112.1| hypothetical protein TSUD_320870, partial [Trifolium subterraneum]
          Length = 629

 Score =  131 bits (330), Expect = 2e-33
 Identities = 76/117 (64%), Positives = 89/117 (76%), Gaps = 1/117 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVP+SSE+RE SN ET  QA IHDS V NV+EGTHSA QPS LKDKMPV A +D +KSSS
Sbjct: 453 LVPLSSEIREISNCETDSQASIHDSVVDNVLEGTHSAQQPSSLKDKMPVPA-TDRLKSSS 511

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSD-VHSSLGKFR 348
           VG PS SQK+LA   ALS SH+   K+ SNV + GVKER  NP +S+ VH+SLGKF+
Sbjct: 512 VGDPSSSQKNLAEGEALSASHK--TKEASNVETCGVKERVENPTSSEGVHNSLGKFK 566


>ref|XP_004508585.1| PREDICTED: uncharacterized protein LOC101510093 isoform X2 [Cicer
           arietinum]
          Length = 685

 Score =  125 bits (315), Expect = 3e-31
 Identities = 75/116 (64%), Positives = 83/116 (71%), Gaps = 1/116 (0%)
 Frame = +1

Query: 4   VPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSSV 183
           VPVS   RE SNYETG QA I D  V NV+EGTHSA QPSG+K KMPV AE+DG+     
Sbjct: 486 VPVS---RETSNYETGSQASIRDMVVDNVLEGTHSAVQPSGVKGKMPVQAETDGL----- 537

Query: 184 GYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINS-DVHSSLGKFR 348
             PS SQKD+AGD ALSVSH+   K+ SNV S G KER  NPINS DVH SLGKF+
Sbjct: 538 --PSSSQKDVAGDEALSVSHK--TKEGSNVESCGAKERVENPINSEDVHKSLGKFK 589


>ref|XP_004508584.1| PREDICTED: uncharacterized protein LOC101510093 isoform X1 [Cicer
           arietinum]
          Length = 686

 Score =  125 bits (315), Expect = 3e-31
 Identities = 75/116 (64%), Positives = 83/116 (71%), Gaps = 1/116 (0%)
 Frame = +1

Query: 4   VPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSSV 183
           VPVS   RE SNYETG QA I D  V NV+EGTHSA QPSG+K KMPV AE+DG+     
Sbjct: 487 VPVS---RETSNYETGSQASIRDMVVDNVLEGTHSAVQPSGVKGKMPVQAETDGL----- 538

Query: 184 GYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINS-DVHSSLGKFR 348
             PS SQKD+AGD ALSVSH+   K+ SNV S G KER  NPINS DVH SLGKF+
Sbjct: 539 --PSSSQKDVAGDEALSVSHK--TKEGSNVESCGAKERVENPINSEDVHKSLGKFK 590


>ref|XP_020226540.1| uncharacterized protein LOC109808107 [Cajanus cajan]
 gb|KYP55638.1| NIPA-like protein [Cajanus cajan]
          Length = 677

 Score =  122 bits (305), Expect = 8e-30
 Identities = 67/117 (57%), Positives = 83/117 (70%), Gaps = 1/117 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVPVSSE+RE SNYE G QA IHDS   + I GT +AGQ S L+DKMP   ++D +  S+
Sbjct: 451 LVPVSSEIREISNYENGSQASIHDS---DFIVGTSNAGQSSSLEDKMPAYTDADKLSGST 507

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINS-DVHSSLGKFR 348
            GYPS SQKD     ALSVSH+ +D  V ++ +  VK+RE NPI+S DVHSSLGKF+
Sbjct: 508 AGYPSSSQKDSTEGEALSVSHKTLDGDVGSLENSVVKDREENPISSEDVHSSLGKFK 564


>ref|XP_003609078.2| C3HC zinc finger-like protein [Medicago truncatula]
 gb|AES91275.2| C3HC zinc finger-like protein [Medicago truncatula]
          Length = 674

 Score =  119 bits (298), Expect = 7e-29
 Identities = 71/118 (60%), Positives = 86/118 (72%), Gaps = 2/118 (1%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQ-AGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSS 177
           LVPVSSE+RE SN ETG Q A I DS + N +EGT SAGQPSG K+KMPV AE+ G+K+S
Sbjct: 465 LVPVSSEIREISNCETGSQQASICDSVLDNDLEGTSSAGQPSGFKEKMPVQAETGGLKNS 524

Query: 178 SVGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPIN-SDVHSSLGKFR 348
           S   PS SQ D+A D AL  SH+   K+ S+V + GVKER  NPIN  DVH+S+GKF+
Sbjct: 525 SAEDPSSSQTDMAEDEAL--SHK--TKEGSHVETSGVKERAENPINREDVHNSIGKFK 578


>ref|XP_003550849.1| PREDICTED: uncharacterized protein LOC100804992 isoform X1 [Glycine
           max]
 gb|KHN10429.1| NIPA-like protein [Glycine soja]
 gb|KRH03832.1| hypothetical protein GLYMA_17G122700 [Glycine max]
          Length = 653

 Score =  117 bits (292), Expect = 4e-28
 Identities = 65/117 (55%), Positives = 81/117 (69%), Gaps = 1/117 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVPVSSE+RE SN+ETG QA +HDS V         AGQ SGLKDKM V  ++D + SS+
Sbjct: 452 LVPVSSEIREISNFETGSQASLHDSDV--------IAGQSSGLKDKMHVHTDADKLNSST 503

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSD-VHSSLGKFR 348
            GYPS SQKD     A  VSH+ +D +V ++ ++GVK+RE NPIN D VHSSLGK +
Sbjct: 504 AGYPSTSQKDSTEGEAPLVSHKTLDGEVGSLENYGVKDREENPINRDNVHSSLGKLK 560


>ref|XP_006600786.1| PREDICTED: uncharacterized protein LOC100804992 isoform X2 [Glycine
           max]
          Length = 569

 Score =  112 bits (279), Expect = 2e-26
 Identities = 63/113 (55%), Positives = 78/113 (69%), Gaps = 1/113 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVPVSSE+RE SN+ETG QA +HDS V         AGQ SGLKDKM V  ++D + SS+
Sbjct: 452 LVPVSSEIREISNFETGSQASLHDSDV--------IAGQSSGLKDKMHVHTDADKLNSST 503

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSD-VHSSL 336
            GYPS SQKD     A  VSH+ +D +V ++ ++GVK+RE NPIN D VHSSL
Sbjct: 504 AGYPSTSQKDSTEGEAPLVSHKTLDGEVGSLENYGVKDREENPINRDNVHSSL 556


>ref|XP_006579725.1| PREDICTED: uncharacterized protein LOC100799942 isoform X2 [Glycine
           max]
 gb|KRH56707.1| hypothetical protein GLYMA_05G014400 [Glycine max]
          Length = 606

 Score =  112 bits (279), Expect = 2e-26
 Identities = 63/117 (53%), Positives = 80/117 (68%), Gaps = 1/117 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVPVSSE+RE SN+ETG Q+ +HDS V         AGQ S LKDKMPV  ++D + SS+
Sbjct: 405 LVPVSSEIREISNFETGSQSSLHDSDV--------LAGQSSALKDKMPVHTDADKLNSSA 456

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSD-VHSSLGKFR 348
            GYPS SQKD     A SVSH+ +D QV ++ ++GVK+RE NPI+ D V  SLGK +
Sbjct: 457 AGYPSSSQKDSTEGEAPSVSHKTLDGQVGSLENYGVKDREENPISRDNVLYSLGKLK 513


>gb|KHN15160.1| Nuclear-interacting partner of ALK [Glycine soja]
          Length = 652

 Score =  112 bits (279), Expect = 3e-26
 Identities = 63/117 (53%), Positives = 80/117 (68%), Gaps = 1/117 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVPVSSE+RE SN+ETG Q+ +HDS V         AGQ S LKDKMPV  ++D + SS+
Sbjct: 451 LVPVSSEIREISNFETGSQSSLHDSDV--------LAGQSSALKDKMPVHTDADKLNSSA 502

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSD-VHSSLGKFR 348
            GYPS SQKD     A SVSH+ +D QV ++ ++GVK+RE NPI+ D V  SLGK +
Sbjct: 503 AGYPSSSQKDSTEGEAPSVSHKTLDGQVGSLENYGVKDREENPISRDNVLYSLGKLK 559


>ref|XP_003525642.1| PREDICTED: uncharacterized protein LOC100799942 isoform X1 [Glycine
           max]
 gb|KRH56706.1| hypothetical protein GLYMA_05G014400 [Glycine max]
          Length = 652

 Score =  112 bits (279), Expect = 3e-26
 Identities = 63/117 (53%), Positives = 80/117 (68%), Gaps = 1/117 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVPVSSE+RE SN+ETG Q+ +HDS V         AGQ S LKDKMPV  ++D + SS+
Sbjct: 451 LVPVSSEIREISNFETGSQSSLHDSDV--------LAGQSSALKDKMPVHTDADKLNSSA 502

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSD-VHSSLGKFR 348
            GYPS SQKD     A SVSH+ +D QV ++ ++GVK+RE NPI+ D V  SLGK +
Sbjct: 503 AGYPSSSQKDSTEGEAPSVSHKTLDGQVGSLENYGVKDREENPISRDNVLYSLGKLK 559


>ref|XP_019439275.1| PREDICTED: uncharacterized protein LOC109344986 isoform X1 [Lupinus
           angustifolius]
 gb|OIW19704.1| hypothetical protein TanjilG_18514 [Lupinus angustifolius]
          Length = 652

 Score =  111 bits (277), Expect = 5e-26
 Identities = 62/115 (53%), Positives = 76/115 (66%), Gaps = 1/115 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVPVSSE+RE S YETG  A IHDS V +VIEGT  AGQPS LKD  PV  E+DG+ S +
Sbjct: 432 LVPVSSEIREISGYETGSIASIHDSVVDDVIEGTSYAGQPSDLKDMAPVHLEADGLNSLA 491

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPIN-SDVHSSLGK 342
            G PS SQK      ALS+S + +D +  +  +HGVK+R  NP N  DV+ S G+
Sbjct: 492 AGDPSSSQKHRTEIEALSISQKTLDGEGISRENHGVKDRVENPANHEDVYCSFGR 546


>ref|XP_019439284.1| PREDICTED: uncharacterized protein LOC109344986 isoform X2 [Lupinus
           angustifolius]
          Length = 631

 Score = 87.4 bits (215), Expect = 1e-17
 Identities = 50/103 (48%), Positives = 63/103 (61%), Gaps = 1/103 (0%)
 Frame = +1

Query: 37  NYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSSVGYPSRSQKDLA 216
           N   G  A IHDS V +VIEGT  AGQPS LKD  PV  E+DG+ S + G PS SQK   
Sbjct: 423 NTVNGSIASIHDSVVDDVIEGTSYAGQPSDLKDMAPVHLEADGLNSLAAGDPSSSQKHRT 482

Query: 217 GDGALSVSHEIVDKQVSNVGSHGVKEREVNPIN-SDVHSSLGK 342
              ALS+S + +D +  +  +HGVK+R  NP N  DV+ S G+
Sbjct: 483 EIEALSISQKTLDGEGISRENHGVKDRVENPANHEDVYCSFGR 525


>ref|XP_007155162.1| hypothetical protein PHAVU_003G178600g [Phaseolus vulgaris]
 gb|ESW27156.1| hypothetical protein PHAVU_003G178600g [Phaseolus vulgaris]
          Length = 655

 Score = 82.0 bits (201), Expect = 9e-16
 Identities = 55/117 (47%), Positives = 68/117 (58%), Gaps = 1/117 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVP SSE RE ++YE G Q  I DS   +VI  TH AGQ   L DKM    ++D + SS+
Sbjct: 456 LVPESSEPRETADYENGSQTRICDS---DVIVDTHHAGQSFCLNDKMTTHTDADKLNSSA 512

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINS-DVHSSLGKFR 348
            GY S +QKD A   ALSV  +  D +V         +RE NPI S DVHSS+ KF+
Sbjct: 513 AGYCSGAQKDSAEGEALSVLRKTSDGKVGT-------DREENPIGSEDVHSSIEKFK 562


>ref|XP_014509169.1| uncharacterized protein LOC106768499 [Vigna radiata var. radiata]
          Length = 655

 Score = 81.6 bits (200), Expect = 1e-15
 Identities = 54/114 (47%), Positives = 68/114 (59%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVP++SE RE ++YETG QA I DS   ++I GT  AGQ   LKDKMPV  ++D + SS+
Sbjct: 457 LVPLTSETRETADYETGSQARICDS---DIIVGTDIAGQSFSLKDKMPVHTDTDKLNSSA 513

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSDVHSSLGK 342
            GY S SQKD     ALSVS +  D  V +       +RE N  + DV SS  K
Sbjct: 514 AGYCSSSQKDSTEGEALSVSRKTSDGVVGS-------DREDNIDSEDVPSSSEK 560


>dbj|BAT76464.1| hypothetical protein VIGAN_01447200 [Vigna angularis var.
           angularis]
          Length = 574

 Score = 77.8 bits (190), Expect = 3e-14
 Identities = 52/114 (45%), Positives = 67/114 (58%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVP++SE RE ++YET  QA I  S   ++I GTH+A Q   LKDKMP   ++D + SS+
Sbjct: 376 LVPLTSETRETADYETSSQARICGS---DIIVGTHNAVQSFSLKDKMPEHTDTDKLNSSA 432

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSDVHSSLGK 342
            GY S SQKD     ALSVS +  D  V +       +RE N  + DV SSL K
Sbjct: 433 AGYCSSSQKDSTEGEALSVSRKTSDGVVGS-------DREDNIDSEDVPSSLEK 479


>ref|XP_017420306.1| PREDICTED: uncharacterized protein LOC108330325 [Vigna angularis]
 gb|KOM33120.1| hypothetical protein LR48_Vigan01g267600 [Vigna angularis]
          Length = 655

 Score = 77.8 bits (190), Expect = 3e-14
 Identities = 52/114 (45%), Positives = 67/114 (58%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           LVP++SE RE ++YET  QA I  S   ++I GTH+A Q   LKDKMP   ++D + SS+
Sbjct: 457 LVPLTSETRETADYETSSQARICGS---DIIVGTHNAVQSFSLKDKMPEHTDTDKLNSSA 513

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPINSDVHSSLGK 342
            GY S SQKD     ALSVS +  D  V +       +RE N  + DV SSL K
Sbjct: 514 AGYCSSSQKDSTEGEALSVSRKTSDGVVGS-------DREDNIDSEDVPSSLEK 560


>ref|XP_016188990.1| uncharacterized protein LOC107630396 [Arachis ipaensis]
          Length = 642

 Score = 58.5 bits (140), Expect = 2e-07
 Identities = 41/113 (36%), Positives = 57/113 (50%), Gaps = 1/113 (0%)
 Frame = +1

Query: 1   LVPVSSEMREFSNYETGPQAGIHDSAVGNVIEGTHSAGQPSGLKDKMPVDAESDGIKSSS 180
           +VP+SSE+RE S+ +TG +A   DS V NVIE TH                E DG+ SS+
Sbjct: 459 IVPMSSEIRESSHDQTGSKASARDSVVDNVIESTHH---------------EGDGLNSSA 503

Query: 181 VGYPSRSQKDLAGDGALSVSHEIVDKQVSNVGSHGVKEREVNPIN-SDVHSSL 336
            G PS  QK+L             D  V+ + ++G K++  N +N  D H SL
Sbjct: 504 EGDPSSLQKNL-------------DGVVNRLSNYGAKDKVENFVNREDQHCSL 543