BLASTX nr result
ID: Astragalus23_contig00022191
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Astragalus23_contig00022191
(391 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|KOM45817.1| hypothetical protein LR48_Vigan06g112300 [Vigna a... 65 2e-12
ref|XP_003517871.2| PREDICTED: lysosomal Pro-X carboxypeptidase-... 66 1e-11
ref|XP_015577579.1| PREDICTED: LOW QUALITY PROTEIN: lysosomal Pr... 70 1e-11
gb|EEF38691.1| lysosomal pro-X carboxypeptidase, putative [Ricin... 70 1e-11
gb|KHN44803.1| Lysosomal Pro-X carboxypeptidase [Glycine soja] 66 1e-11
ref|XP_006573176.1| PREDICTED: lysosomal Pro-X carboxypeptidase-... 66 1e-11
ref|XP_004487553.1| PREDICTED: lysosomal Pro-X carboxypeptidase ... 69 6e-11
ref|XP_019176061.1| PREDICTED: lysosomal Pro-X carboxypeptidase-... 61 9e-11
ref|XP_019176091.1| PREDICTED: lysosomal Pro-X carboxypeptidase-... 62 1e-10
ref|XP_016170001.1| lysosomal Pro-X carboxypeptidase [Arachis ip... 62 1e-10
ref|XP_015935952.1| lysosomal Pro-X carboxypeptidase [Arachis du... 62 1e-10
dbj|BAT99185.1| hypothetical protein VIGAN_10058100 [Vigna angul... 65 2e-10
ref|XP_017426987.1| PREDICTED: lysosomal Pro-X carboxypeptidase-... 65 2e-10
ref|XP_022756642.1| lysosomal Pro-X carboxypeptidase-like [Durio... 61 2e-10
ref|XP_002523750.1| PREDICTED: lysosomal Pro-X carboxypeptidase ... 66 2e-10
gb|KVI00106.1| Peptidase S28 [Cynara cardunculus var. scolymus] 61 2e-10
ref|XP_020257553.1| lysosomal Pro-X carboxypeptidase-like isofor... 60 3e-10
ref|XP_020257554.1| lysosomal Pro-X carboxypeptidase-like isofor... 60 3e-10
gb|ONK75724.1| uncharacterized protein A4U43_C03F19880 [Asparagu... 60 3e-10
ref|XP_014497335.1| lysosomal Pro-X carboxypeptidase [Vigna radi... 60 3e-10
>gb|KOM45817.1| hypothetical protein LR48_Vigan06g112300 [Vigna angularis]
Length = 420
Score = 65.5 bits (158), Expect(2) = 2e-12
Identities = 34/66 (51%), Positives = 45/66 (68%), Gaps = 5/66 (7%)
Frame = -2
Query: 258 PFQ-KWVLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTDYYA 94
PF K VL +ISDS+VAV+T NG+ C DL QSDP+WL++Q +EE+ RW+ YYA
Sbjct: 347 PFNLKRVLENISDSIVAVHTVNGSHCLDLLRANQSDPEWLVKQRKEEMKIIKRWIAQYYA 406
Query: 93 KLNPPK 76
L+ K
Sbjct: 407 DLHALK 412
Score = 33.9 bits (76), Expect(2) = 2e-12
Identities = 18/36 (50%), Positives = 21/36 (58%)
Frame = -1
Query: 391 CKGIDEAAFGKYTLSKIYGGMVAYQGLRDIWVLGAT 284
C GID A+ G LSKIY G+VA +G V G T
Sbjct: 279 CGGIDGASLGSDILSKIYAGVVALKGNTTCTVNGPT 314
>ref|XP_003517871.2| PREDICTED: lysosomal Pro-X carboxypeptidase-like isoform X1
[Glycine max]
gb|KRH75150.1| hypothetical protein GLYMA_01G065400 [Glycine max]
Length = 503
Score = 65.9 bits (159), Expect(2) = 1e-11
Identities = 32/62 (51%), Positives = 44/62 (70%), Gaps = 4/62 (6%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTDYYAKLNPPK 76
VL++ISDS+VAV+T NG+ C DL QSDP WL++Q ++EV RW+T YYA L+ K
Sbjct: 436 VLDNISDSIVAVHTVNGSHCLDLLRANQSDPGWLVEQRKKEVKIIKRWITQYYADLDALK 495
Query: 75 SQ 70
+
Sbjct: 496 DK 497
Score = 31.2 bits (69), Expect(2) = 1e-11
Identities = 14/18 (77%), Positives = 15/18 (83%)
Frame = -3
Query: 296 LGSNIIFSNCLRDPFRSG 243
LGSNIIFSN LRDP+ G
Sbjct: 417 LGSNIIFSNGLRDPYSIG 434
>ref|XP_015577579.1| PREDICTED: LOW QUALITY PROTEIN: lysosomal Pro-X carboxypeptidase
[Ricinus communis]
Length = 492
Score = 70.1 bits (170), Expect(2) = 1e-11
Identities = 32/56 (57%), Positives = 40/56 (71%), Gaps = 4/56 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEVR----WLTDYYAKL 88
VL ISDS+VAVYT +GA C DLYP T DP WL++Q +E++ WL +YYAKL
Sbjct: 434 VLEDISDSIVAVYTEHGAHCLDLYPSTPDDPNWLVEQREKEIKIIAAWLAEYYAKL 489
Score = 26.9 bits (58), Expect(2) = 1e-11
Identities = 14/25 (56%), Positives = 18/25 (72%), Gaps = 1/25 (4%)
Frame = -3
Query: 314 LKRHLG-LGSNIIFSNCLRDPFRSG 243
+K LG SNIIF+N LRDP+ +G
Sbjct: 408 IKTVLGNFASNIIFANGLRDPWSAG 432
>gb|EEF38691.1| lysosomal pro-X carboxypeptidase, putative [Ricinus communis]
Length = 489
Score = 70.1 bits (170), Expect(2) = 1e-11
Identities = 32/56 (57%), Positives = 40/56 (71%), Gaps = 4/56 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEVR----WLTDYYAKL 88
VL ISDS+VAVYT +GA C DLYP T DP WL++Q +E++ WL +YYAKL
Sbjct: 431 VLEDISDSIVAVYTEHGAHCLDLYPSTPDDPNWLVEQREKEIKIIAAWLAEYYAKL 486
Score = 26.9 bits (58), Expect(2) = 1e-11
Identities = 14/25 (56%), Positives = 18/25 (72%), Gaps = 1/25 (4%)
Frame = -3
Query: 314 LKRHLG-LGSNIIFSNCLRDPFRSG 243
+K LG SNIIF+N LRDP+ +G
Sbjct: 405 IKTVLGNFASNIIFANGLRDPWSAG 429
>gb|KHN44803.1| Lysosomal Pro-X carboxypeptidase [Glycine soja]
Length = 471
Score = 65.9 bits (159), Expect(2) = 1e-11
Identities = 32/62 (51%), Positives = 44/62 (70%), Gaps = 4/62 (6%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTDYYAKLNPPK 76
VL++ISDS+VAV+T NG+ C DL QSDP WL++Q ++EV RW+T YYA L+ K
Sbjct: 404 VLDNISDSIVAVHTVNGSHCLDLLRANQSDPGWLVEQRKKEVKIIKRWITQYYADLDALK 463
Query: 75 SQ 70
+
Sbjct: 464 DK 465
Score = 31.2 bits (69), Expect(2) = 1e-11
Identities = 14/18 (77%), Positives = 15/18 (83%)
Frame = -3
Query: 296 LGSNIIFSNCLRDPFRSG 243
LGSNIIFSN LRDP+ G
Sbjct: 385 LGSNIIFSNGLRDPYSIG 402
>ref|XP_006573176.1| PREDICTED: lysosomal Pro-X carboxypeptidase-like isoform X2
[Glycine max]
Length = 444
Score = 65.9 bits (159), Expect(2) = 1e-11
Identities = 32/62 (51%), Positives = 44/62 (70%), Gaps = 4/62 (6%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTDYYAKLNPPK 76
VL++ISDS+VAV+T NG+ C DL QSDP WL++Q ++EV RW+T YYA L+ K
Sbjct: 377 VLDNISDSIVAVHTVNGSHCLDLLRANQSDPGWLVEQRKKEVKIIKRWITQYYADLDALK 436
Query: 75 SQ 70
+
Sbjct: 437 DK 438
Score = 31.2 bits (69), Expect(2) = 1e-11
Identities = 14/18 (77%), Positives = 15/18 (83%)
Frame = -3
Query: 296 LGSNIIFSNCLRDPFRSG 243
LGSNIIFSN LRDP+ G
Sbjct: 358 LGSNIIFSNGLRDPYSIG 375
>ref|XP_004487553.1| PREDICTED: lysosomal Pro-X carboxypeptidase [Cicer arietinum]
Length = 506
Score = 68.9 bits (167), Expect = 6e-11
Identities = 35/65 (53%), Positives = 46/65 (70%), Gaps = 5/65 (7%)
Frame = -2
Query: 267 LKRPFQKW-VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTD 103
LK P+ VL+++SDSL+AVYTTNG+ C D+ QSDPKWLIQQ ++EV RW+
Sbjct: 433 LKDPYSTGGVLSNLSDSLIAVYTTNGSHCLDILQTRQSDPKWLIQQRKKEVEIIKRWIEQ 492
Query: 102 YYAKL 88
YY+ L
Sbjct: 493 YYSDL 497
>ref|XP_019176061.1| PREDICTED: lysosomal Pro-X carboxypeptidase-like [Ipomoea nil]
Length = 495
Score = 60.8 bits (146), Expect(2) = 9e-11
Identities = 31/69 (44%), Positives = 45/69 (65%), Gaps = 5/69 (7%)
Frame = -2
Query: 267 LKRPFQKW-VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTD 103
L+ PF VL +ISD+++AVYT NG+ C D+ ++++DP WL +Q + EV RW+T
Sbjct: 426 LRDPFSSGGVLENISDTILAVYTRNGSHCLDILGESKTDPDWLTEQRKREVEIIRRWITK 485
Query: 102 YYAKLNPPK 76
YYA L K
Sbjct: 486 YYADLQALK 494
Score = 33.1 bits (74), Expect(2) = 9e-11
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN LRDPF SG
Sbjct: 417 GSNIIFSNGLRDPFSSG 433
>ref|XP_019176091.1| PREDICTED: lysosomal Pro-X carboxypeptidase-like [Ipomoea nil]
Length = 499
Score = 61.6 bits (148), Expect(2) = 1e-10
Identities = 30/60 (50%), Positives = 41/60 (68%), Gaps = 4/60 (6%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTDYYAKLNPPK 76
VL SISD+++AVYT NG+ C DL + ++DP WL +Q + EV RW+T+YYA L K
Sbjct: 439 VLKSISDTILAVYTRNGSHCLDLQGERETDPDWLTEQRKTEVEIIQRWITNYYADLQALK 498
Score = 32.0 bits (71), Expect(2) = 1e-10
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN LRDP+ SG
Sbjct: 421 GSNIIFSNGLRDPYSSG 437
>ref|XP_016170001.1| lysosomal Pro-X carboxypeptidase [Arachis ipaensis]
Length = 494
Score = 61.6 bits (148), Expect(2) = 1e-10
Identities = 27/56 (48%), Positives = 40/56 (71%), Gaps = 4/56 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEVR----WLTDYYAKL 88
VLN+ISDSL+AV+T NG+ C D+ ++DP WL++Q + E++ W+T YYA L
Sbjct: 438 VLNNISDSLIAVHTVNGSHCLDMLGAKKTDPNWLVEQRKTEIKIMKGWITQYYADL 493
Score = 32.0 bits (71), Expect(2) = 1e-10
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN LRDP+ SG
Sbjct: 420 GSNIIFSNGLRDPYSSG 436
>ref|XP_015935952.1| lysosomal Pro-X carboxypeptidase [Arachis duranensis]
Length = 491
Score = 61.6 bits (148), Expect(2) = 1e-10
Identities = 27/56 (48%), Positives = 40/56 (71%), Gaps = 4/56 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEVR----WLTDYYAKL 88
VLN+ISDSL+AV+T NG+ C D+ ++DP WL++Q + E++ W+T YYA L
Sbjct: 435 VLNNISDSLIAVHTANGSHCLDMLGAKKTDPNWLVEQRKTEIKIMKGWITQYYADL 490
Score = 32.0 bits (71), Expect(2) = 1e-10
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN LRDP+ SG
Sbjct: 417 GSNIIFSNGLRDPYSSG 433
>dbj|BAT99185.1| hypothetical protein VIGAN_10058100 [Vigna angularis var.
angularis]
Length = 508
Score = 64.7 bits (156), Expect(2) = 2e-10
Identities = 34/69 (49%), Positives = 46/69 (66%), Gaps = 5/69 (7%)
Frame = -2
Query: 267 LKRPFQ-KWVLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTD 103
LK P+ VL +ISDS+VAV+T NG+ C DL QSDP+WL++Q +EE+ RW+
Sbjct: 432 LKDPYSIGGVLENISDSIVAVHTVNGSHCLDLLRANQSDPEWLVKQRKEEMKIIKRWIAQ 491
Query: 102 YYAKLNPPK 76
YYA L+ K
Sbjct: 492 YYADLHALK 500
Score = 28.5 bits (62), Expect(2) = 2e-10
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN L+DP+ G
Sbjct: 423 GSNIIFSNGLKDPYSIG 439
>ref|XP_017426987.1| PREDICTED: lysosomal Pro-X carboxypeptidase-like isoform X1 [Vigna
angularis]
Length = 503
Score = 64.7 bits (156), Expect(2) = 2e-10
Identities = 34/69 (49%), Positives = 46/69 (66%), Gaps = 5/69 (7%)
Frame = -2
Query: 267 LKRPFQ-KWVLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTD 103
LK P+ VL +ISDS+VAV+T NG+ C DL QSDP+WL++Q +EE+ RW+
Sbjct: 427 LKDPYSIGGVLENISDSIVAVHTVNGSHCLDLLRANQSDPEWLVKQRKEEMKIIKRWIAQ 486
Query: 102 YYAKLNPPK 76
YYA L+ K
Sbjct: 487 YYADLHALK 495
Score = 28.5 bits (62), Expect(2) = 2e-10
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN L+DP+ G
Sbjct: 418 GSNIIFSNGLKDPYSIG 434
>ref|XP_022756642.1| lysosomal Pro-X carboxypeptidase-like [Durio zibethinus]
Length = 522
Score = 60.8 bits (146), Expect(2) = 2e-10
Identities = 27/56 (48%), Positives = 40/56 (71%), Gaps = 4/56 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEVR----WLTDYYAKL 88
VL +ISDS+VAVY+ NG+ C D+ P+ SDP+WL++Q + EV+ W++ YY L
Sbjct: 458 VLENISDSVVAVYSANGSHCLDILPEKNSDPQWLVEQRKTEVQIIQCWISKYYTDL 513
Score = 32.0 bits (71), Expect(2) = 2e-10
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -3
Query: 299 GLGSNIIFSNCLRDPFRSG 243
G SNIIFSN LRDP+ SG
Sbjct: 438 GFASNIIFSNGLRDPYSSG 456
>ref|XP_002523750.1| PREDICTED: lysosomal Pro-X carboxypeptidase [Ricinus communis]
gb|EEF38690.1| Lysosomal Pro-X carboxypeptidase, putative [Ricinus communis]
Length = 491
Score = 65.9 bits (159), Expect(2) = 2e-10
Identities = 30/57 (52%), Positives = 41/57 (71%), Gaps = 4/57 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEVR----WLTDYYAKLN 85
VL ISD++VAVYT GA C DLYP T +DP WL++Q +E++ W+ +YYAKL+
Sbjct: 433 VLEDISDTVVAVYTEYGAHCLDLYPPTPNDPDWLVEQRDKEIKIIAAWIAEYYAKLS 489
Score = 26.9 bits (58), Expect(2) = 2e-10
Identities = 14/25 (56%), Positives = 18/25 (72%), Gaps = 1/25 (4%)
Frame = -3
Query: 314 LKRHLG-LGSNIIFSNCLRDPFRSG 243
+K LG SNIIF+N LRDP+ +G
Sbjct: 407 IKTVLGNFASNIIFANGLRDPWSAG 431
>gb|KVI00106.1| Peptidase S28 [Cynara cardunculus var. scolymus]
Length = 456
Score = 60.8 bits (146), Expect(2) = 2e-10
Identities = 29/56 (51%), Positives = 39/56 (69%), Gaps = 4/56 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTDYYAKL 88
VL +ISD ++AVYTTNG+ C D+ +++DP WLI+Q +EV RWLT YY L
Sbjct: 396 VLENISDEVLAVYTTNGSHCLDILASSKTDPDWLIKQRNDEVKIIRRWLTKYYHDL 451
Score = 32.0 bits (71), Expect(2) = 2e-10
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN LRDP+ SG
Sbjct: 378 GSNIIFSNGLRDPYSSG 394
>ref|XP_020257553.1| lysosomal Pro-X carboxypeptidase-like isoform X1 [Asparagus
officinalis]
Length = 544
Score = 60.5 bits (145), Expect(2) = 3e-10
Identities = 28/56 (50%), Positives = 39/56 (69%), Gaps = 4/56 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTDYYAKL 88
VL +ISD++VA+YTT G+ C D+ P T +DP+WLI Q EEV RW+ +Y A +
Sbjct: 487 VLCNISDTIVAIYTTQGSHCLDILPATPADPEWLIAQRNEEVKIIERWIAEYKANI 542
Score = 32.0 bits (71), Expect(2) = 3e-10
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN LRDP+ SG
Sbjct: 469 GSNIIFSNGLRDPYSSG 485
>ref|XP_020257554.1| lysosomal Pro-X carboxypeptidase-like isoform X2 [Asparagus
officinalis]
Length = 500
Score = 60.5 bits (145), Expect(2) = 3e-10
Identities = 28/56 (50%), Positives = 39/56 (69%), Gaps = 4/56 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTDYYAKL 88
VL +ISD++VA+YTT G+ C D+ P T +DP+WLI Q EEV RW+ +Y A +
Sbjct: 443 VLCNISDTIVAIYTTQGSHCLDILPATPADPEWLIAQRNEEVKIIERWIAEYKANI 498
Score = 32.0 bits (71), Expect(2) = 3e-10
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN LRDP+ SG
Sbjct: 425 GSNIIFSNGLRDPYSSG 441
>gb|ONK75724.1| uncharacterized protein A4U43_C03F19880 [Asparagus officinalis]
Length = 485
Score = 60.5 bits (145), Expect(2) = 3e-10
Identities = 28/56 (50%), Positives = 39/56 (69%), Gaps = 4/56 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEV----RWLTDYYAKL 88
VL +ISD++VA+YTT G+ C D+ P T +DP+WLI Q EEV RW+ +Y A +
Sbjct: 428 VLCNISDTIVAIYTTQGSHCLDILPATPADPEWLIAQRNEEVKIIERWIAEYKANI 483
Score = 32.0 bits (71), Expect(2) = 3e-10
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN LRDP+ SG
Sbjct: 410 GSNIIFSNGLRDPYSSG 426
>ref|XP_014497335.1| lysosomal Pro-X carboxypeptidase [Vigna radiata var. radiata]
Length = 504
Score = 60.1 bits (144), Expect(2) = 3e-10
Identities = 28/56 (50%), Positives = 41/56 (73%), Gaps = 4/56 (7%)
Frame = -2
Query: 243 VLNSISDSLVAVYTTNGARCHDLYPKTQSDPKWLIQQ*REEVR----WLTDYYAKL 88
VL +IS+S+VAV T NG+ C D+ KT+ DP+WL++Q +EEV+ W+T+Y A L
Sbjct: 434 VLENISNSVVAVTTVNGSHCLDIQSKTEKDPEWLVRQRKEEVKIMKGWITEYEADL 489
Score = 32.0 bits (71), Expect(2) = 3e-10
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -3
Query: 293 GSNIIFSNCLRDPFRSG 243
GSNIIFSN LRDP+ SG
Sbjct: 416 GSNIIFSNGLRDPYSSG 432