BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00019722
         (1884 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_001347975.1| syringolide-induced protein B13-1-1 [Glycine...   995   0.0  
dbj|BAB86897.1| syringolide-induced protein B13-1-1, partial [Gl...   994   0.0  
ref|XP_020236942.1| L-ascorbate oxidase [Cajanus cajan] >gi|1012...   994   0.0  
ref|XP_003630068.1| L-ascorbate oxidase [Medicago truncatula] >g...   991   0.0  
gb|KRG88449.1| hypothetical protein GLYMA_U040600 [Glycine max]       990   0.0  
ref|XP_004504046.1| PREDICTED: L-ascorbate oxidase [Cicer arieti...   983   0.0  
ref|XP_014507036.1| L-ascorbate oxidase isoform X2 [Vigna radiat...   981   0.0  
gb|PNY10248.1| L-ascorbate oxidase [Trifolium pratense]               979   0.0  
ref|XP_017431795.1| PREDICTED: L-ascorbate oxidase [Vigna angula...   979   0.0  
ref|XP_007159725.1| hypothetical protein PHAVU_002G262200g [Phas...   973   0.0  
ref|XP_022641730.1| L-ascorbate oxidase isoform X1 [Vigna radiat...   967   0.0  
gb|KHN29089.1| L-ascorbate oxidase [Glycine soja]                     963   0.0  
ref|XP_019462684.1| PREDICTED: L-ascorbate oxidase [Lupinus angu...   941   0.0  
ref|XP_015956011.1| L-ascorbate oxidase [Arachis duranensis]          926   0.0  
ref|XP_020975046.1| L-ascorbate oxidase isoform X2 [Arachis ipae...   926   0.0  
ref|XP_016189927.1| L-ascorbate oxidase isoform X1 [Arachis ipae...   926   0.0  
ref|XP_024194634.1| L-ascorbate oxidase-like [Rosa chinensis] >g...   920   0.0  
ref|XP_011097600.1| L-ascorbate oxidase [Sesamum indicum]             918   0.0  
ref|XP_021816393.1| L-ascorbate oxidase-like [Prunus avium]           914   0.0  
ref|XP_008233457.2| PREDICTED: L-ascorbate oxidase isoform X1 [P...   913   0.0  

>ref|NP_001347975.1| syringolide-induced protein B13-1-1 [Glycine max]
 gb|KHN38199.1| L-ascorbate oxidase [Glycine soja]
          Length = 577

 Score =  995 bits (2572), Expect = 0.0
 Identities = 464/577 (80%), Positives = 515/577 (89%), Gaps = 12/577 (2%)
 Frame = +3

Query: 45   MVKLQLS--------LLVLCFFFILST----EAKIRHYKWEVKYEYKSLDCFKKLVITVN 188
            MV+L+LS        LL+LCFF IL      EA+IRH+KWEVKYE++S DCFKKLVIT+N
Sbjct: 1    MVELRLSIRALQLPTLLILCFFLILGNFHKAEARIRHHKWEVKYEFRSPDCFKKLVITIN 60

Query: 189  GRTPGPTIQAQEGDTVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDI 368
            G+TPGPTIQAQEGDT+VV+V N L+TENL+IHWHGIRQIGTPWFDGTEGV+QCPILPGD 
Sbjct: 61   GKTPGPTIQAQEGDTIVVQVNNSLVTENLSIHWHGIRQIGTPWFDGTEGVTQCPILPGDT 120

Query: 369  FVYKFVVDRPGTYLYHAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTY 548
            F+Y+FVVDRPGTYLYHAHYGMQREAGLYG+IRV P +PEPFA+D DRSIILNDWYH+STY
Sbjct: 121  FIYQFVVDRPGTYLYHAHYGMQREAGLYGMIRVAPRDPEPFAYDLDRSIILNDWYHKSTY 180

Query: 549  EQAAGLSSIPFQWVGEPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKT 728
            EQAAGLSSIPFQWVGEPQSLLIHGKGRFNCS +P++   VC++SNP+CSPF+ TVIPGKT
Sbjct: 181  EQAAGLSSIPFQWVGEPQSLLIHGKGRFNCSKSPSVSTDVCDTSNPQCSPFVQTVIPGKT 240

Query: 729  YRLRVASLTSLSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPS 908
            YRLR+ASLT+LSALSF+IEGHNMTVVEADGHYVEPF V+NLFIYSGETYSVLVK +Q+PS
Sbjct: 241  YRLRIASLTALSALSFEIEGHNMTVVEADGHYVEPFEVKNLFIYSGETYSVLVKTDQDPS 300

Query: 909  KNYWITSNVVSRNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAH 1088
            +NYWITSNVVSRNRTTP GL +FNYY                AWD VEPR+AQSL+IKA 
Sbjct: 301  RNYWITSNVVSRNRTTPPGLGMFNYYPNHPKRSPPTVPPSPPAWDDVEPRLAQSLSIKAR 360

Query: 1089 QGYIKKPPTTSDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTP 1268
            QGYI KPPTTSDRVIVLLNTQN I+ +RHWSVNNVSF+LPHTPYLI+LK NI GAFD TP
Sbjct: 361  QGYILKPPTTSDRVIVLLNTQNNISEYRHWSVNNVSFTLPHTPYLISLKENITGAFDPTP 420

Query: 1269 PPDGYDFVNYDIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHG 1448
            PPDGYDF NYDIF VA+N+NAT+S+GIYRLKFNTTVD+ILQNANTMNKNNSETHPWHLHG
Sbjct: 421  PPDGYDFANYDIFSVASNANATSSSGIYRLKFNTTVDIILQNANTMNKNNSETHPWHLHG 480

Query: 1449 HDFWVLGYGKGKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIE 1628
            HDFWVLGYGKGKFD  NDTK YNL NPIMKNTVPVHPFGWTALRFR+DNPGVWAFHCHIE
Sbjct: 481  HDFWVLGYGKGKFDVNNDTKKYNLENPIMKNTVPVHPFGWTALRFRTDNPGVWAFHCHIE 540

Query: 1629 SHFYMGMGVVFEEGIDRVGKLPSSIMGCGKSKGFLRP 1739
            SHFYMGMGVVFEEGI+RVGKLPSSIMGCG+++GF RP
Sbjct: 541  SHFYMGMGVVFEEGIERVGKLPSSIMGCGQTRGFHRP 577


>dbj|BAB86897.1| syringolide-induced protein B13-1-1, partial [Glycine max]
          Length = 567

 Score =  994 bits (2570), Expect = 0.0
 Identities = 462/567 (81%), Positives = 512/567 (90%), Gaps = 5/567 (0%)
 Frame = +3

Query: 54   LQL-SLLVLCFFFILST----EAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQA 218
            LQL +LL+LCFF IL      EA+IRH+KWEVKYE++S DCFKKLVIT+NG+TPGPTIQA
Sbjct: 1    LQLPTLLILCFFLILGNFHKAEARIRHHKWEVKYEFRSPDCFKKLVITINGKTPGPTIQA 60

Query: 219  QEGDTVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRP 398
            QEGDT+VV+V N L+TENL+IHWHGIRQIGTPWFDGTEGV+QCPILPGD F+Y+FVVDRP
Sbjct: 61   QEGDTIVVQVNNSLVTENLSIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFIYQFVVDRP 120

Query: 399  GTYLYHAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIP 578
            GTYLYHAHYGMQREAGLYG+IRV P +PEPFA+D DRSIILNDWYH+STYEQAAGLSSIP
Sbjct: 121  GTYLYHAHYGMQREAGLYGMIRVAPRDPEPFAYDLDRSIILNDWYHKSTYEQAAGLSSIP 180

Query: 579  FQWVGEPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVASLTS 758
            FQWVGEPQSLLIHGKGRFNCS +P++   VC++SNP+CSPF+ TVIPGKTYRLR+ASLT+
Sbjct: 181  FQWVGEPQSLLIHGKGRFNCSKSPSVSTDVCDTSNPQCSPFVQTVIPGKTYRLRIASLTA 240

Query: 759  LSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVV 938
            LSALSF+IEGHNMTVVEADGHYVEPF V+NLFIYSGETYSVLVK +Q+PS+NYWITSNVV
Sbjct: 241  LSALSFEIEGHNMTVVEADGHYVEPFEVKNLFIYSGETYSVLVKTDQDPSRNYWITSNVV 300

Query: 939  SRNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKPPTT 1118
            SRNRTTP GL +FNYY                AWD VEPR+AQSL+IKA QGYI KPPTT
Sbjct: 301  SRNRTTPPGLGMFNYYPNHPKRSPPTVPPSPPAWDDVEPRLAQSLSIKARQGYILKPPTT 360

Query: 1119 SDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDFVNY 1298
            SDRVIVLLNTQN I+ +RHWSVNNVSF+LPHTPYLI+LK NI GAFD TPPPDGYDF NY
Sbjct: 361  SDRVIVLLNTQNNISEYRHWSVNNVSFTLPHTPYLISLKENITGAFDPTPPPDGYDFANY 420

Query: 1299 DIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGK 1478
            DIF VA+N+NAT+S+GIYRLKFNTTVD+ILQNANTMNKNNSETHPWHLHGHDFWVLGYGK
Sbjct: 421  DIFSVASNANATSSSGIYRLKFNTTVDIILQNANTMNKNNSETHPWHLHGHDFWVLGYGK 480

Query: 1479 GKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVV 1658
            GKFD  NDTK YNL NPIMKNTVPVHPFGWTALRFR+DNPGVWAFHCHIESHFYMGMGVV
Sbjct: 481  GKFDVNNDTKKYNLENPIMKNTVPVHPFGWTALRFRTDNPGVWAFHCHIESHFYMGMGVV 540

Query: 1659 FEEGIDRVGKLPSSIMGCGKSKGFLRP 1739
            FEEGI+RVGKLPSSIMGCG+++GF RP
Sbjct: 541  FEEGIERVGKLPSSIMGCGQTRGFHRP 567


>ref|XP_020236942.1| L-ascorbate oxidase [Cajanus cajan]
 gb|KYP45224.1| L-ascorbate oxidase [Cajanus cajan]
          Length = 576

 Score =  994 bits (2569), Expect = 0.0
 Identities = 466/576 (80%), Positives = 510/576 (88%), Gaps = 11/576 (1%)
 Frame = +3

Query: 45   MVKLQL------SLLVLCFFFILST-----EAKIRHYKWEVKYEYKSLDCFKKLVITVNG 191
            MVKL L      +LL+LCFF +L       EA+IRHYKWEVKYE+++ DCFKKLVIT+NG
Sbjct: 1    MVKLHLCSRAMPTLLLLCFFLVLGNFNKLAEARIRHYKWEVKYEFRAPDCFKKLVITING 60

Query: 192  RTPGPTIQAQEGDTVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIF 371
             TPGPTIQAQEGDT+VV+V N L+TENLAIHWHGIRQIGTPWFDGTEGV+QCPI PGD F
Sbjct: 61   ETPGPTIQAQEGDTIVVQVNNSLVTENLAIHWHGIRQIGTPWFDGTEGVTQCPIFPGDTF 120

Query: 372  VYKFVVDRPGTYLYHAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTYE 551
            +Y+FVVDRPGTYLYHAHYGMQREAGLYGLIRVTP +PEPFA+D DR+IILNDWYHQSTYE
Sbjct: 121  IYRFVVDRPGTYLYHAHYGMQREAGLYGLIRVTPRDPEPFAYDLDRTIILNDWYHQSTYE 180

Query: 552  QAAGLSSIPFQWVGEPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKTY 731
            QAAGLSSIPFQWVGEPQSLLI+GKGRFNCS +P+    VC++SNP+CSPF+ TVIPGKTY
Sbjct: 181  QAAGLSSIPFQWVGEPQSLLINGKGRFNCSKSPSSGTDVCDTSNPQCSPFVQTVIPGKTY 240

Query: 732  RLRVASLTSLSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSK 911
            RLR+ASLT+LSALSFQIE HNMTVVEADGHYVEPFVV+NLFIYSGETYSVLVK+N+ PS+
Sbjct: 241  RLRIASLTALSALSFQIEDHNMTVVEADGHYVEPFVVKNLFIYSGETYSVLVKSNREPSR 300

Query: 912  NYWITSNVVSRNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQ 1091
            NYWITSNVVSRNR+TPAGL IFNYY                AWD  +PR+AQSL+IKA Q
Sbjct: 301  NYWITSNVVSRNRSTPAGLGIFNYYPNHPKRSPPTGPPSPPAWDDAKPRLAQSLSIKARQ 360

Query: 1092 GYIKKPPTTSDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPP 1271
            GYI KPP TSDR+IVLLNTQN I+G+RHWSVNNVSF+LPHTPYLIALK NINGAFD TPP
Sbjct: 361  GYIHKPPATSDRLIVLLNTQNNISGYRHWSVNNVSFTLPHTPYLIALKENINGAFDTTPP 420

Query: 1272 PDGYDFVNYDIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGH 1451
            PDGYDF +YDIF V NN NAT+SNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGH
Sbjct: 421  PDGYDFASYDIFSVPNNVNATSSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGH 480

Query: 1452 DFWVLGYGKGKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIES 1631
            DFWVLGYG+GKFD KNDTK YNL NPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIES
Sbjct: 481  DFWVLGYGEGKFDVKNDTKKYNLENPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIES 540

Query: 1632 HFYMGMGVVFEEGIDRVGKLPSSIMGCGKSKGFLRP 1739
            HFYMGMGVVFEEG++RVGKLPSSIMGCG +KGF  P
Sbjct: 541  HFYMGMGVVFEEGVERVGKLPSSIMGCGATKGFHTP 576


>ref|XP_003630068.1| L-ascorbate oxidase [Medicago truncatula]
 gb|AET04544.1| L-ascorbate oxidase [Medicago truncatula]
          Length = 577

 Score =  991 bits (2561), Expect = 0.0
 Identities = 472/574 (82%), Positives = 508/574 (88%), Gaps = 11/574 (1%)
 Frame = +3

Query: 45   MVKLQLSL------LVLCFFFIL----STEAKIRHYKWEVKYEYKSLDCFKKLVITVNGR 194
            MV+LQLSL      L LCFF IL      EA +RHYKWEVKY+Y+S DC+KKLVIT+NG+
Sbjct: 1    MVQLQLSLRAAPKLLFLCFFLILVNFHKAEAGVRHYKWEVKYDYRSPDCYKKLVITINGK 60

Query: 195  TPGPTIQAQEGDTVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFV 374
            TPGPTIQAQEGDTVVVEV NKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGD FV
Sbjct: 61   TPGPTIQAQEGDTVVVEVNNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDTFV 120

Query: 375  YKFVVDRPGTYLYHAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTYEQ 554
            Y+FVVDRPGTYLYHAHYGMQREAG+YG+IRV P +PEPF++DFDRSIILNDWYH+STYEQ
Sbjct: 121  YRFVVDRPGTYLYHAHYGMQREAGVYGMIRVAPNDPEPFSYDFDRSIILNDWYHRSTYEQ 180

Query: 555  AAGLSSIPFQWVGEPQSLLIHGKGRFNCSTTPTIQA-GVCNSSNPECSPFLLTVIPGKTY 731
            +A LS+IPFQWVGEP SLLIHGKGR+NCS  P++ A GVCNSSNPECSPF  TV+ GKTY
Sbjct: 181  SARLSAIPFQWVGEPDSLLIHGKGRYNCSLLPSLPAAGVCNSSNPECSPFSQTVVSGKTY 240

Query: 732  RLRVASLTSLSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSK 911
            R+RVASLT+LSALSFQIEGHNMTVVEADGHYV+PFVV+NL+IYSGETYSVLVK +Q+PS+
Sbjct: 241  RIRVASLTALSALSFQIEGHNMTVVEADGHYVDPFVVKNLYIYSGETYSVLVKTDQDPSR 300

Query: 912  NYWITSNVVSRNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQ 1091
            NYWITSNVVSRNRTTP GL IFNYY                AWD+VE R  QSLAIKAHQ
Sbjct: 301  NYWITSNVVSRNRTTPPGLGIFNYYPNHPMRSPPTSPPPPPAWDNVESRHTQSLAIKAHQ 360

Query: 1092 GYIKKPPTTSDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPP 1271
             Y  KPPTTSDRVIVLLNTQNTI+  RHWSVNNVSF LPHTPYL+ALK NI G F+QTPP
Sbjct: 361  NYTIKPPTTSDRVIVLLNTQNTIDNVRHWSVNNVSFFLPHTPYLVALKENITGEFNQTPP 420

Query: 1272 PDGYDFVNYDIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGH 1451
            PDGYDF NYDIF VANN+NAT+SNGIYRLKFNTTVD+ILQNANTMNKNNSETHPWHLHGH
Sbjct: 421  PDGYDFNNYDIFSVANNTNATSSNGIYRLKFNTTVDIILQNANTMNKNNSETHPWHLHGH 480

Query: 1452 DFWVLGYGKGKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIES 1631
            DFWVLGYGKGKFDA  D KNYNLVNPIMKNTVPVH FGWTALRFRSDNPGVWAFHCHIES
Sbjct: 481  DFWVLGYGKGKFDANKDPKNYNLVNPIMKNTVPVHSFGWTALRFRSDNPGVWAFHCHIES 540

Query: 1632 HFYMGMGVVFEEGIDRVGKLPSSIMGCGKSKGFL 1733
            HFYMGMGVVFEEGI+RVGKLPSSIMGCGKSKGFL
Sbjct: 541  HFYMGMGVVFEEGIERVGKLPSSIMGCGKSKGFL 574


>gb|KRG88449.1| hypothetical protein GLYMA_U040600 [Glycine max]
          Length = 577

 Score =  990 bits (2559), Expect = 0.0
 Identities = 462/577 (80%), Positives = 514/577 (89%), Gaps = 12/577 (2%)
 Frame = +3

Query: 45   MVKLQLS--------LLVLCFFFILST----EAKIRHYKWEVKYEYKSLDCFKKLVITVN 188
            MV+L+LS        LL+LCFF IL      EA+IRH+KWEVKYE++S DCFKKLVIT+N
Sbjct: 1    MVELRLSIRALQLPTLLILCFFLILGNFHKAEARIRHHKWEVKYEFRSPDCFKKLVITIN 60

Query: 189  GRTPGPTIQAQEGDTVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDI 368
            G+TPGPTIQAQEGDT+VV+V N L+TENL+IHWHGIRQIGTPWFDGTEGV+QCPILPGD 
Sbjct: 61   GKTPGPTIQAQEGDTIVVQVNNSLVTENLSIHWHGIRQIGTPWFDGTEGVTQCPILPGDT 120

Query: 369  FVYKFVVDRPGTYLYHAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTY 548
            F+Y+FVVDRPGTYLYHAHYGMQREAGLYG+IRV P +PEPFA+D DRSIILNDWYH+STY
Sbjct: 121  FIYQFVVDRPGTYLYHAHYGMQREAGLYGMIRVAPRDPEPFAYDLDRSIILNDWYHKSTY 180

Query: 549  EQAAGLSSIPFQWVGEPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKT 728
            EQAAGLSSIPFQWVGEPQSLLIHGKGRFNCS +P++   VC++SNP+CSPF+ TVIPGKT
Sbjct: 181  EQAAGLSSIPFQWVGEPQSLLIHGKGRFNCSKSPSVSTDVCDTSNPQCSPFVQTVIPGKT 240

Query: 729  YRLRVASLTSLSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPS 908
            YRLR+ASLT+LSALSF+IE +NMTVVEADGHYVEPF V+NLFIYSGETYSVLVK +Q+PS
Sbjct: 241  YRLRIASLTALSALSFEIEANNMTVVEADGHYVEPFEVKNLFIYSGETYSVLVKTDQDPS 300

Query: 909  KNYWITSNVVSRNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAH 1088
            +NYWITSNVVSRNRTTP GL +FNYY                AWD VEPR+AQSL+IKA 
Sbjct: 301  RNYWITSNVVSRNRTTPPGLGMFNYYPNHPKRSPPTVPPSPPAWDDVEPRLAQSLSIKAR 360

Query: 1089 QGYIKKPPTTSDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTP 1268
            QGYI KPPTTSDRVIVLLNTQN I+ +RHWSVNNVSF+LPHTPYLI+LK NI GAFD TP
Sbjct: 361  QGYILKPPTTSDRVIVLLNTQNNISEYRHWSVNNVSFTLPHTPYLISLKENITGAFDPTP 420

Query: 1269 PPDGYDFVNYDIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHG 1448
            PPDGYDF NYDIF VA+N+NAT+S+GIYRLKFNTTVD+ILQNANTMNKNNSETHPWHLHG
Sbjct: 421  PPDGYDFANYDIFSVASNANATSSSGIYRLKFNTTVDIILQNANTMNKNNSETHPWHLHG 480

Query: 1449 HDFWVLGYGKGKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIE 1628
            HDFWVLGYGKGKFD  NDTK YNL NPIMKNTVPVHPFGWTALRFR+DNPGVWAFHCHIE
Sbjct: 481  HDFWVLGYGKGKFDVNNDTKKYNLENPIMKNTVPVHPFGWTALRFRTDNPGVWAFHCHIE 540

Query: 1629 SHFYMGMGVVFEEGIDRVGKLPSSIMGCGKSKGFLRP 1739
            SHFYMGMGVVFEEGI+RVGKLPSSIMGCG+++GF RP
Sbjct: 541  SHFYMGMGVVFEEGIERVGKLPSSIMGCGQTRGFHRP 577


>ref|XP_004504046.1| PREDICTED: L-ascorbate oxidase [Cicer arietinum]
          Length = 572

 Score =  983 bits (2540), Expect = 0.0
 Identities = 468/571 (81%), Positives = 506/571 (88%), Gaps = 10/571 (1%)
 Frame = +3

Query: 45   MVKLQLSL------LVLCFFFIL----STEAKIRHYKWEVKYEYKSLDCFKKLVITVNGR 194
            MV+LQL+L      LVLCFF I+      EA +RHYK EVKY+Y+S DC+KKLVIT+NG+
Sbjct: 1    MVQLQLNLRALPKLLVLCFFLIIWNFYKAEAGVRHYKCEVKYDYRSPDCYKKLVITINGQ 60

Query: 195  TPGPTIQAQEGDTVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFV 374
            TPGPTI+AQEGDTV+VEV N LLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGD FV
Sbjct: 61   TPGPTIEAQEGDTVIVEVNNDLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDTFV 120

Query: 375  YKFVVDRPGTYLYHAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTYEQ 554
            Y+FVVDRPGTYLYHAHYGMQREAGLYG+IRV P EPEPF +DFDRSIILNDWYHQSTYEQ
Sbjct: 121  YQFVVDRPGTYLYHAHYGMQREAGLYGMIRVAPNEPEPFTYDFDRSIILNDWYHQSTYEQ 180

Query: 555  AAGLSSIPFQWVGEPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKTYR 734
            AA LSSIPFQWVGEPQSLLIHGKGRFNCS TP+++A VCNSSNP+CSPF+ TVI GKTYR
Sbjct: 181  AALLSSIPFQWVGEPQSLLIHGKGRFNCSLTPSLKADVCNSSNPQCSPFVQTVITGKTYR 240

Query: 735  LRVASLTSLSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKN 914
            LRVASLT+LSALSFQIEGHNMTVVEADGHYV+PFVVQNL+IYSGETYSV+VK +++PS+N
Sbjct: 241  LRVASLTALSALSFQIEGHNMTVVEADGHYVDPFVVQNLYIYSGETYSVIVKTDKDPSRN 300

Query: 915  YWITSNVVSRNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQG 1094
            YWITSNVVSRNRTTP GLAIFNYY                AWD+V+ R+AQSL IKAHQ 
Sbjct: 301  YWITSNVVSRNRTTPPGLAIFNYYPNHPMRSPPTPPPSPPAWDNVDSRLAQSLKIKAHQS 360

Query: 1095 YIKKPPTTSDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPP 1274
            YI KPPTTSDRVIV+LNTQN+I+G+RHWSVNNVSF LPHTPYLIALK N    F QT PP
Sbjct: 361  YINKPPTTSDRVIVMLNTQNSIDGYRHWSVNNVSFFLPHTPYLIALKENFTDEFSQTSPP 420

Query: 1275 DGYDFVNYDIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHD 1454
            D YD  NYDIF VANN+NAT+SNGIYRL+FN TVDVILQNANTMNKNNSETHPWHLHGHD
Sbjct: 421  DSYD-SNYDIFSVANNTNATSSNGIYRLQFNITVDVILQNANTMNKNNSETHPWHLHGHD 479

Query: 1455 FWVLGYGKGKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESH 1634
            FWVLGYGKGKFD  NDTKNYNLVNPI KNTVPVHPFGWTALRFRSDNPGVWAFHCHIESH
Sbjct: 480  FWVLGYGKGKFDVNNDTKNYNLVNPIRKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESH 539

Query: 1635 FYMGMGVVFEEGIDRVGKLPSSIMGCGKSKG 1727
            FYMGMGV+FEEGIDRVGKLPSSIMGCGKSKG
Sbjct: 540  FYMGMGVIFEEGIDRVGKLPSSIMGCGKSKG 570


>ref|XP_014507036.1| L-ascorbate oxidase isoform X2 [Vigna radiata var. radiata]
          Length = 570

 Score =  981 bits (2536), Expect = 0.0
 Identities = 460/559 (82%), Positives = 501/559 (89%)
 Frame = +3

Query: 63   SLLVLCFFFILSTEAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEGDTVVV 242
            +LL+LCFF   + EA+ RHYKW VKYEY+S DCFKKLVIT+NG TPGPTIQAQEGDTVVV
Sbjct: 14   TLLLLCFFLFHNAEARNRHYKWNVKYEYRSPDCFKKLVITINGNTPGPTIQAQEGDTVVV 73

Query: 243  EVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPGTYLYHAH 422
            +V N LLTENL+IHWHGIRQIGTPWFDGTEGV+QCPILPGD FVY+FVVDRPGTYLYHAH
Sbjct: 74   QVNNTLLTENLSIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYRFVVDRPGTYLYHAH 133

Query: 423  YGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIPFQWVGEPQ 602
            YGMQREAGLYGLIRV   +PEPF +DFDRSIILNDWYH+STYEQAAGLSSIPFQWVGEP+
Sbjct: 134  YGMQREAGLYGLIRVAARDPEPFTYDFDRSIILNDWYHKSTYEQAAGLSSIPFQWVGEPE 193

Query: 603  SLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVASLTSLSALSFQI 782
            SLLIHGKGRFNCS +P+++  VC+ S  +CSPF+ TVIPGKTYRLR+ SLT+LSALSFQI
Sbjct: 194  SLLIHGKGRFNCSQSPSLKTDVCDMS--KCSPFVQTVIPGKTYRLRIGSLTALSALSFQI 251

Query: 783  EGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVVSRNRTTPA 962
            EGHNMTVVEADGHYVEPFVV+NLFIYSGETYSVLVK +Q+PS+NYWITSNVVSRNRTTPA
Sbjct: 252  EGHNMTVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWITSNVVSRNRTTPA 311

Query: 963  GLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKPPTTSDRVIVLL 1142
            GL IFNYY                AWD VEPR+AQSLAIKA QGYI+KPPTTSDRVIVLL
Sbjct: 312  GLGIFNYYPNHPKRSPPTVPPSPPAWDDVEPRLAQSLAIKARQGYIQKPPTTSDRVIVLL 371

Query: 1143 NTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDFVNYDIFKVANN 1322
            NTQN I+ +RHWSVNNVSF+LPHTPYLIALK NI  AFD TPP DG++F NYDIF V+ N
Sbjct: 372  NTQNNISEYRHWSVNNVSFTLPHTPYLIALKENITNAFDPTPPSDGHEFANYDIFNVSPN 431

Query: 1323 SNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFDAKND 1502
            +NAT+SNGIYRLKFNTTVD+ILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFDA ND
Sbjct: 432  ANATSSNGIYRLKFNTTVDLILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFDANND 491

Query: 1503 TKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEGIDRV 1682
            TK YNL NPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEGI+RV
Sbjct: 492  TKKYNLENPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEGIERV 551

Query: 1683 GKLPSSIMGCGKSKGFLRP 1739
            GKLPSSIMGCG++K F RP
Sbjct: 552  GKLPSSIMGCGQTKSFHRP 570


>gb|PNY10248.1| L-ascorbate oxidase [Trifolium pratense]
          Length = 581

 Score =  979 bits (2531), Expect = 0.0
 Identities = 466/579 (80%), Positives = 505/579 (87%), Gaps = 15/579 (2%)
 Frame = +3

Query: 45   MVKLQL-------SLLVLCFFFILST----EAKIRHYKWEVKYEYKSLDCFKKLVITVNG 191
            MV+ QL        LLVL FFFIL      EA +RHYKWEVKYEYKS DC+KKLVIT+NG
Sbjct: 1    MVQFQLLSLRAAPKLLVLFFFFILVNFHIAEAGVRHYKWEVKYEYKSPDCYKKLVITING 60

Query: 192  RTPGPTIQAQEGDTVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIF 371
            ++PGPTIQAQEGDTV+VEV NKLLTENLAIHWHGIRQIGTPWFDGTEGV+QCPILPGD F
Sbjct: 61   KSPGPTIQAQEGDTVIVEVNNKLLTENLAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTF 120

Query: 372  VYKFVVDRPGTYLYHAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTYE 551
            VY+FVVDRPGTYLYHAHYGMQREAGLYG+IRV P +PEPF++DFDRSIILNDWYH S +E
Sbjct: 121  VYQFVVDRPGTYLYHAHYGMQREAGLYGMIRVAPNDPEPFSYDFDRSIILNDWYHSSAFE 180

Query: 552  QAAGLSSIPFQWVGEPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKTY 731
            QAA LSSIPFQWVGEPQSLLIHGKG+F CS +P+  AGVCN +NPECSPF+ TV+ GKTY
Sbjct: 181  QAALLSSIPFQWVGEPQSLLIHGKGKFECSLSPSSAAGVCNKTNPECSPFVQTVVSGKTY 240

Query: 732  RLRVASLTSLSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSK 911
            R+RVASLT+LSALSF+IEGHNMTVVEADGHYVEPFVV+NL+IYSGETYSVLVK NQ+PS+
Sbjct: 241  RIRVASLTALSALSFEIEGHNMTVVEADGHYVEPFVVKNLYIYSGETYSVLVKTNQDPSR 300

Query: 912  NYWITSNVVSRNRTT----PAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAI 1079
            NYWITSNVV RNRT     P GLAIFNYY                AWD+ + R+AQSLAI
Sbjct: 301  NYWITSNVVGRNRTAPPGIPPGLAIFNYYPNHPKRQPPTSPPTPPAWDNKDSRLAQSLAI 360

Query: 1080 KAHQGYIKKPPTTSDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFD 1259
            KAHQ Y  KPPTTSDRVIV+LNTQNTI+GFRHWSVNNVS SLPHTPYL+ALK NI G F+
Sbjct: 361  KAHQNYTVKPPTTSDRVIVMLNTQNTIDGFRHWSVNNVSLSLPHTPYLVALKENITGVFN 420

Query: 1260 QTPPPDGYDFVNYDIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWH 1439
            QTPPPDGYDF NYDIF VANNSNAT+SNGIYRL+FN TVDVILQNANTM+KNNSETHPWH
Sbjct: 421  QTPPPDGYDFNNYDIFSVANNSNATSSNGIYRLQFNITVDVILQNANTMSKNNSETHPWH 480

Query: 1440 LHGHDFWVLGYGKGKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHC 1619
            LHGHDFWVLGYG GKFD  N  KNYNLVNPIMKNTVP+HPFGWTALRFRSDNPGVWAFHC
Sbjct: 481  LHGHDFWVLGYGNGKFDVNNHPKNYNLVNPIMKNTVPMHPFGWTALRFRSDNPGVWAFHC 540

Query: 1620 HIESHFYMGMGVVFEEGIDRVGKLPSSIMGCGKSKGFLR 1736
            HIESHFYMGMGVVFEEGIDRVGKLPSSIMGCGK+KG LR
Sbjct: 541  HIESHFYMGMGVVFEEGIDRVGKLPSSIMGCGKTKGLLR 579


>ref|XP_017431795.1| PREDICTED: L-ascorbate oxidase [Vigna angularis]
 gb|KOM30682.1| hypothetical protein LR48_Vigan01g023600 [Vigna angularis]
 dbj|BAT73361.1| hypothetical protein VIGAN_01083600 [Vigna angularis var. angularis]
          Length = 574

 Score =  979 bits (2531), Expect = 0.0
 Identities = 460/563 (81%), Positives = 504/563 (89%), Gaps = 4/563 (0%)
 Frame = +3

Query: 63   SLLVLCFFFIL----STEAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEGD 230
            +LL+LCFF IL    + EA+ RHYKW VKYE++S DCFKKLVIT+NG TPGPTIQA+EGD
Sbjct: 14   TLLLLCFFLILGNFRNAEARNRHYKWNVKYEFRSPDCFKKLVITINGNTPGPTIQAEEGD 73

Query: 231  TVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPGTYL 410
            TVVV+V N LLTENL+IHWHGIRQIGTPWFDGTEGV+QCPILPGD FVY+FVVDRPGTYL
Sbjct: 74   TVVVQVNNTLLTENLSIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYRFVVDRPGTYL 133

Query: 411  YHAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIPFQWV 590
            YHAHYGMQREAGLYGLIRV   +PEPF +DFDRSIILNDWYH+STYEQAAGLSSIPFQWV
Sbjct: 134  YHAHYGMQREAGLYGLIRVAARDPEPFTYDFDRSIILNDWYHKSTYEQAAGLSSIPFQWV 193

Query: 591  GEPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVASLTSLSAL 770
            GEP+SLLIHGKGRFNCS +P+++  VC+ S  +CSPF+ TVIPGKTYRLR+ SLT+LSAL
Sbjct: 194  GEPESLLIHGKGRFNCSQSPSLRTDVCDMS--KCSPFVQTVIPGKTYRLRIGSLTALSAL 251

Query: 771  SFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVVSRNR 950
            SFQIEGHNMTVVEADGHYVEPFVV+NLFIYSGETYSVLVK +Q+PS+NYWITSNVVSRNR
Sbjct: 252  SFQIEGHNMTVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWITSNVVSRNR 311

Query: 951  TTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKPPTTSDRV 1130
            TTP GL IFNYY                AWD VEPR+AQSLAIKA QGYI+KPPTTSDRV
Sbjct: 312  TTPPGLGIFNYYPNHPKRSPPTVPPSPPAWDDVEPRLAQSLAIKARQGYIQKPPTTSDRV 371

Query: 1131 IVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDFVNYDIFK 1310
            I+LLNTQN I+ +RHWSVNNVSF+LPHTPYLIALK NI  AFD TPPPDG++F NYDIF 
Sbjct: 372  ILLLNTQNNISEYRHWSVNNVSFTLPHTPYLIALKENITNAFDPTPPPDGHEFANYDIFN 431

Query: 1311 VANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFD 1490
            V+ N+NAT+SNGIYRLKFNTTVD+ILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFD
Sbjct: 432  VSPNANATSSNGIYRLKFNTTVDLILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFD 491

Query: 1491 AKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEG 1670
            A NDTK YNL NPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEG
Sbjct: 492  ANNDTKKYNLENPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEG 551

Query: 1671 IDRVGKLPSSIMGCGKSKGFLRP 1739
            I+RVGKLPSSIMGCG++KGF RP
Sbjct: 552  IERVGKLPSSIMGCGQTKGFHRP 574


>ref|XP_007159725.1| hypothetical protein PHAVU_002G262200g [Phaseolus vulgaris]
 gb|ESW31719.1| hypothetical protein PHAVU_002G262200g [Phaseolus vulgaris]
          Length = 573

 Score =  973 bits (2516), Expect = 0.0
 Identities = 460/563 (81%), Positives = 498/563 (88%), Gaps = 4/563 (0%)
 Frame = +3

Query: 63   SLLVLCFFFIL----STEAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEGD 230
            +L +LCFF I     + EA+ RHYKWEVKYEY+S DCFKKLVITVNG TPGPTIQAQEGD
Sbjct: 13   TLFILCFFLIFGNFRNAEARNRHYKWEVKYEYRSPDCFKKLVITVNGITPGPTIQAQEGD 72

Query: 231  TVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPGTYL 410
            TVVV+V N L+TENL+IHWHGIRQIGTPWFDG+EGV+QCPILPGD F+Y+FVVDRPGTYL
Sbjct: 73   TVVVQVNNTLITENLSIHWHGIRQIGTPWFDGSEGVTQCPILPGDTFIYQFVVDRPGTYL 132

Query: 411  YHAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIPFQWV 590
            YHAHYGMQREAGLYGLIRV P +PEPF +DFDRSIILNDWYH+STYEQAAGLSSIPFQWV
Sbjct: 133  YHAHYGMQREAGLYGLIRVAPRDPEPFNYDFDRSIILNDWYHKSTYEQAAGLSSIPFQWV 192

Query: 591  GEPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVASLTSLSAL 770
            GEPQSLLIHGKGRFNCS +P++   VC+ S  +CSPF+ TVIPGKTYRLR+ SLT+LSAL
Sbjct: 193  GEPQSLLIHGKGRFNCSKSPSLSTDVCDIS--KCSPFIQTVIPGKTYRLRICSLTALSAL 250

Query: 771  SFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVVSRNR 950
            SFQIEGHNMTVVEADGHYVEPFVV+NLFIYSGETYSVLVK +Q+PS+NYWITSNVVSRNR
Sbjct: 251  SFQIEGHNMTVVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWITSNVVSRNR 310

Query: 951  TTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKPPTTSDRV 1130
            TTP GLAIFNYY                AWD VEPR+AQSLAIKA QGYI KPPTTSDRV
Sbjct: 311  TTPPGLAIFNYYPNHPKRSPPTVPPSPPAWDDVEPRLAQSLAIKARQGYIHKPPTTSDRV 370

Query: 1131 IVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDFVNYDIFK 1310
            I+LLNTQN ++  RHWSVNNVSF+ PHTPYLIALK NI  AFD T PPDG +F NYDIF 
Sbjct: 371  IMLLNTQNNVSERRHWSVNNVSFTHPHTPYLIALKENITNAFDPTSPPDGSEFANYDIFS 430

Query: 1311 VANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFD 1490
            VA N+NATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFD
Sbjct: 431  VAENANATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFD 490

Query: 1491 AKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEG 1670
             KNDTK YNL NPIMKNTVPVHPFGWTA+RFRSDNPGVWAFHCHIESHFYMGMGVVFEEG
Sbjct: 491  VKNDTKQYNLENPIMKNTVPVHPFGWTAVRFRSDNPGVWAFHCHIESHFYMGMGVVFEEG 550

Query: 1671 IDRVGKLPSSIMGCGKSKGFLRP 1739
            I+RVGKLPSSIMGCG++KGF  P
Sbjct: 551  IERVGKLPSSIMGCGQAKGFHNP 573


>ref|XP_022641730.1| L-ascorbate oxidase isoform X1 [Vigna radiata var. radiata]
          Length = 594

 Score =  967 bits (2501), Expect = 0.0
 Identities = 460/583 (78%), Positives = 501/583 (85%), Gaps = 24/583 (4%)
 Frame = +3

Query: 63   SLLVLCFFFILSTEAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEGDTVVV 242
            +LL+LCFF   + EA+ RHYKW VKYEY+S DCFKKLVIT+NG TPGPTIQAQEGDTVVV
Sbjct: 14   TLLLLCFFLFHNAEARNRHYKWNVKYEYRSPDCFKKLVITINGNTPGPTIQAQEGDTVVV 73

Query: 243  EVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDR--------- 395
            +V N LLTENL+IHWHGIRQIGTPWFDGTEGV+QCPILPGD FVY+FVVDR         
Sbjct: 74   QVNNTLLTENLSIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYRFVVDRVSYHVNSLS 133

Query: 396  ---------------PGTYLYHAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDW 530
                           PGTYLYHAHYGMQREAGLYGLIRV   +PEPF +DFDRSIILNDW
Sbjct: 134  SWFLKSDLVESLMLQPGTYLYHAHYGMQREAGLYGLIRVAARDPEPFTYDFDRSIILNDW 193

Query: 531  YHQSTYEQAAGLSSIPFQWVGEPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLT 710
            YH+STYEQAAGLSSIPFQWVGEP+SLLIHGKGRFNCS +P+++  VC+ S  +CSPF+ T
Sbjct: 194  YHKSTYEQAAGLSSIPFQWVGEPESLLIHGKGRFNCSQSPSLKTDVCDMS--KCSPFVQT 251

Query: 711  VIPGKTYRLRVASLTSLSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVK 890
            VIPGKTYRLR+ SLT+LSALSFQIEGHNMTVVEADGHYVEPFVV+NLFIYSGETYSVLVK
Sbjct: 252  VIPGKTYRLRIGSLTALSALSFQIEGHNMTVVEADGHYVEPFVVKNLFIYSGETYSVLVK 311

Query: 891  ANQNPSKNYWITSNVVSRNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQS 1070
             +Q+PS+NYWITSNVVSRNRTTPAGL IFNYY                AWD VEPR+AQS
Sbjct: 312  TDQDPSRNYWITSNVVSRNRTTPAGLGIFNYYPNHPKRSPPTVPPSPPAWDDVEPRLAQS 371

Query: 1071 LAIKAHQGYIKKPPTTSDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNING 1250
            LAIKA QGYI+KPPTTSDRVIVLLNTQN I+ +RHWSVNNVSF+LPHTPYLIALK NI  
Sbjct: 372  LAIKARQGYIQKPPTTSDRVIVLLNTQNNISEYRHWSVNNVSFTLPHTPYLIALKENITN 431

Query: 1251 AFDQTPPPDGYDFVNYDIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETH 1430
            AFD TPP DG++F NYDIF V+ N+NAT+SNGIYRLKFNTTVD+ILQNANTMNKNNSETH
Sbjct: 432  AFDPTPPSDGHEFANYDIFNVSPNANATSSNGIYRLKFNTTVDLILQNANTMNKNNSETH 491

Query: 1431 PWHLHGHDFWVLGYGKGKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWA 1610
            PWHLHGHDFWVLGYGKGKFDA NDTK YNL NPIMKNTVPVHPFGWTALRFRSDNPGVWA
Sbjct: 492  PWHLHGHDFWVLGYGKGKFDANNDTKKYNLENPIMKNTVPVHPFGWTALRFRSDNPGVWA 551

Query: 1611 FHCHIESHFYMGMGVVFEEGIDRVGKLPSSIMGCGKSKGFLRP 1739
            FHCHIESHFYMGMGVVFEEGI+RVGKLPSSIMGCG++K F RP
Sbjct: 552  FHCHIESHFYMGMGVVFEEGIERVGKLPSSIMGCGQTKSFHRP 594


>gb|KHN29089.1| L-ascorbate oxidase [Glycine soja]
          Length = 576

 Score =  963 bits (2490), Expect = 0.0
 Identities = 447/562 (79%), Positives = 499/562 (88%), Gaps = 4/562 (0%)
 Frame = +3

Query: 66   LLVLCFFFILST----EAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEGDT 233
            LL+LCFF IL      EA+IRHY WE KYE++S DCFKKLVIT+NG+TPGP+IQAQEGDT
Sbjct: 17   LLILCFFVILGNFHKAEARIRHYIWEAKYEFRSPDCFKKLVITINGKTPGPSIQAQEGDT 76

Query: 234  VVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPGTYLY 413
            ++V+V N L+TENL+IHWHGIRQIGTPWFDGTEGV+QCPILPGD F+Y+FVVDRPGTYLY
Sbjct: 77   IIVQVNNSLVTENLSIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFIYQFVVDRPGTYLY 136

Query: 414  HAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIPFQWVG 593
            HAHYGMQREAGLYG++RV P +PEPFA+D DRSIILNDWYH STYEQAAGLSSIPF+WVG
Sbjct: 137  HAHYGMQREAGLYGMMRVAPRDPEPFAYDLDRSIILNDWYHSSTYEQAAGLSSIPFRWVG 196

Query: 594  EPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVASLTSLSALS 773
            EPQSLLIHGKG FNCS +P++   VC++S  +CSPF+ TVIPGKTYRLR+ASLT+LSALS
Sbjct: 197  EPQSLLIHGKGIFNCSKSPSLSTDVCDAS--KCSPFVQTVIPGKTYRLRIASLTALSALS 254

Query: 774  FQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVVSRNRT 953
            FQIEGHNMTVVEADGHYVEPFVV+ LFIYSGETYSV VK++Q+PS+NYWITSNVVSRNR+
Sbjct: 255  FQIEGHNMTVVEADGHYVEPFVVKKLFIYSGETYSVTVKSDQDPSRNYWITSNVVSRNRS 314

Query: 954  TPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKPPTTSDRVI 1133
            TPAGL +FNYY                AWD VEPR+AQS +IKA QGYI KPPTTSDRVI
Sbjct: 315  TPAGLGMFNYYPNHPKRSPPTVPPSPPAWDDVEPRLAQSFSIKARQGYIHKPPTTSDRVI 374

Query: 1134 VLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDFVNYDIFKV 1313
            VLLNTQN I+ +RHWSVNNVSF+LPHTPYLIALK NINGAFD T PPDGYDF NYDIF V
Sbjct: 375  VLLNTQNNISEYRHWSVNNVSFTLPHTPYLIALKENINGAFDSTSPPDGYDFANYDIFSV 434

Query: 1314 ANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFDA 1493
            A+N+NAT+S+GIYRLKFNTTVD+ILQNANTM K NSETHPWHLHGHDFWVLGYGKGKFD 
Sbjct: 435  ASNANATSSSGIYRLKFNTTVDIILQNANTMTKTNSETHPWHLHGHDFWVLGYGKGKFDV 494

Query: 1494 KNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEGI 1673
             NDTK YNL NPIMKNTVPVHPFGWTALRFR+DNPGVWAFHCHIESHFYMGMGVVFEEG+
Sbjct: 495  NNDTKKYNLENPIMKNTVPVHPFGWTALRFRTDNPGVWAFHCHIESHFYMGMGVVFEEGV 554

Query: 1674 DRVGKLPSSIMGCGKSKGFLRP 1739
            +RVGKLPSSIMGCG+++GF  P
Sbjct: 555  ERVGKLPSSIMGCGQTRGFHGP 576


>ref|XP_019462684.1| PREDICTED: L-ascorbate oxidase [Lupinus angustifolius]
          Length = 571

 Score =  941 bits (2433), Expect = 0.0
 Identities = 442/560 (78%), Positives = 485/560 (86%), Gaps = 8/560 (1%)
 Frame = +3

Query: 66   LLVLCFFFIL----STEAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEGDT 233
            L  +C  FIL    + EA++RHYKWEVKYEYKS DCFKKLVIT+NGRTPGPTI AQEGDT
Sbjct: 12   LFFVCVLFILGNLYTAEARVRHYKWEVKYEYKSPDCFKKLVITINGRTPGPTIHAQEGDT 71

Query: 234  VVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPGTYLY 413
            V+V+VTN L TENLAIHWHGIRQIGTPW DGTEGV+QCP+LPGD F Y+FVVDRPGTYLY
Sbjct: 72   VIVDVTNNLGTENLAIHWHGIRQIGTPWSDGTEGVTQCPVLPGDTFKYQFVVDRPGTYLY 131

Query: 414  HAHYGMQREAGLYGLIRVTPLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIPFQWVG 593
            HAHYGMQR AGLYG I+V P +PEPFA+DFDRSIILNDWYHQSTYEQA GLSS PF WVG
Sbjct: 132  HAHYGMQRGAGLYGSIQVAPNDPEPFAYDFDRSIILNDWYHQSTYEQATGLSSNPFVWVG 191

Query: 594  EPQSLLIHGKGRFNCSTTPTIQ----AGVCNSSNPECSPFLLTVIPGKTYRLRVASLTSL 761
            EPQSLLI+GKGRFNCST  +       GVCN+SNP+CSP++ TVI GKTYR+R+ SLT L
Sbjct: 192  EPQSLLINGKGRFNCSTISSSSLSSTGGVCNTSNPDCSPYVQTVISGKTYRIRIGSLTGL 251

Query: 762  SALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVVS 941
            SALSFQIEGHNMTVVEADGH+VEPFVV+NLFIYSGETYSVLVKA+Q+PS+NYWITSNVV+
Sbjct: 252  SALSFQIEGHNMTVVEADGHFVEPFVVKNLFIYSGETYSVLVKADQDPSRNYWITSNVVA 311

Query: 942  RNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKPPTTS 1121
            RNRTTP GLA FNYY                AWD  EPR+AQSLAIKA +GY+ K PTTS
Sbjct: 312  RNRTTPPGLATFNYYPNHPKRSPPTSPPTPPAWDDAEPRMAQSLAIKARRGYVNKAPTTS 371

Query: 1122 DRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDFVNYD 1301
            DRVIV LNTQNT++GFR WSVNNVSFSLPHTPYLIALKHN+  +F  TPPPDGYDF NYD
Sbjct: 372  DRVIVFLNTQNTLDGFRRWSVNNVSFSLPHTPYLIALKHNLKNSFHSTPPPDGYDFANYD 431

Query: 1302 IFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKG 1481
            IF VANN+NAT++NGIYRLKFNTTVD+ILQNANTM KNNSETHPWHLHGHDFWVLGYGKG
Sbjct: 432  IFSVANNTNATSTNGIYRLKFNTTVDIILQNANTMTKNNSETHPWHLHGHDFWVLGYGKG 491

Query: 1482 KFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVF 1661
            KFD  ND K YNL NPIMKNTV VHPFGWTALRFR++NPGVWAFHCHIE+HFYMGMGVVF
Sbjct: 492  KFDKDNDPKKYNLENPIMKNTVVVHPFGWTALRFRANNPGVWAFHCHIEAHFYMGMGVVF 551

Query: 1662 EEGIDRVGKLPSSIMGCGKS 1721
            EEGI RVGKLPSSIMGCG++
Sbjct: 552  EEGIHRVGKLPSSIMGCGRT 571


>ref|XP_015956011.1| L-ascorbate oxidase [Arachis duranensis]
          Length = 566

 Score =  926 bits (2394), Expect = 0.0
 Identities = 429/544 (78%), Positives = 485/544 (89%), Gaps = 1/544 (0%)
 Frame = +3

Query: 102  EAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEGDTVVVEVTNKLLTENLAI 281
            E +IRHY+W+VKYEYKS DCFKKLVIT+NGR+PGPTIQA+EGDTVVVEV N L+TENLAI
Sbjct: 22   EGRIRHYRWDVKYEYKSPDCFKKLVITINGRSPGPTIQAEEGDTVVVEVNNSLVTENLAI 81

Query: 282  HWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPGTYLYHAHYGMQREAGLYGLI 461
            HWHGIRQIG+PWFDGTEGV+QCP+LPGD F Y+FVVD+ GTYLYHAHYGMQREAGLYGLI
Sbjct: 82   HWHGIRQIGSPWFDGTEGVTQCPVLPGDTFKYQFVVDQAGTYLYHAHYGMQREAGLYGLI 141

Query: 462  RVTPLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIPFQWVGEPQSLLIHGKGRFNCS 641
            RV+P +PEPFA+D+DRSIIL+DW+H+STYEQAAGLSSIPFQWVGEPQSLLI+GKGRFNCS
Sbjct: 142  RVSPRQPEPFAYDYDRSIILDDWFHKSTYEQAAGLSSIPFQWVGEPQSLLINGKGRFNCS 201

Query: 642  TTPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVASLTSLSALSFQIEGHNMTVVEADGH 821
             +  +  G+CN++NPEC+P+  TV+PGKTYRLR+ SLT+LSALSFQI+GH M VVEADGH
Sbjct: 202  LSSPV-TGICNATNPECAPYSQTVVPGKTYRLRIGSLTTLSALSFQIQGHKMRVVEADGH 260

Query: 822  YVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVVSRNRTTPAGLAIFNYYXXXXX 1001
            YVEPF V+NLFIYSGETYSVLVKA+Q+P +NYWITSNVVSRNRTTP GLAIFNYY     
Sbjct: 261  YVEPFEVENLFIYSGETYSVLVKADQDPKRNYWITSNVVSRNRTTPPGLAIFNYYPNHPM 320

Query: 1002 XXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKPPTTSDRVIVLLNTQNTINGFRHWS 1181
                       AWD V+PR+AQSLAIKA QG+I+KPPTTSDRVIVLLNTQNTING+R WS
Sbjct: 321  RSPPTPPPTPPAWDDVKPRLAQSLAIKARQGFIQKPPTTSDRVIVLLNTQNTINGYRKWS 380

Query: 1182 VNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDFVNYDIFKVANNSNATTSNGIYRLK 1361
            VNNVSFSLP TPYL+ALK N+  AFDQTPPP+GYD VNYDIF V NN+NAT+SNGIYRL+
Sbjct: 381  VNNVSFSLPQTPYLVALKDNLKDAFDQTPPPEGYDSVNYDIFSVQNNTNATSSNGIYRLE 440

Query: 1362 FNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFDAKNDTKNYNLVNPIMKN 1541
            FN+TVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYG GKFD   D K YNL NPI+KN
Sbjct: 441  FNSTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGNGKFDKDKDPKKYNLENPILKN 500

Query: 1542 TVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEGIDRVG-KLPSSIMGCGK 1718
            TVPVHP+GWTALRF+++NPGVW FHCHI+SHFYMGMGVVFEEGI R+  KLPSSIMGCG+
Sbjct: 501  TVPVHPYGWTALRFKANNPGVWLFHCHIDSHFYMGMGVVFEEGIHRLAEKLPSSIMGCGE 560

Query: 1719 SKGF 1730
            +K F
Sbjct: 561  TKTF 564


>ref|XP_020975046.1| L-ascorbate oxidase isoform X2 [Arachis ipaensis]
          Length = 566

 Score =  926 bits (2393), Expect = 0.0
 Identities = 430/544 (79%), Positives = 485/544 (89%), Gaps = 1/544 (0%)
 Frame = +3

Query: 102  EAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEGDTVVVEVTNKLLTENLAI 281
            E +IRHY+W+VKYEYKS DCFKKLVITVNGR+PGPTIQA+EGDTVVVEV N L+TENLAI
Sbjct: 22   EGRIRHYRWDVKYEYKSPDCFKKLVITVNGRSPGPTIQAEEGDTVVVEVNNSLVTENLAI 81

Query: 282  HWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPGTYLYHAHYGMQREAGLYGLI 461
            HWHGIRQIG+PWFDGTEGV+QCP+LPGD F Y+FVVD+ GTYLYHAHYGMQREAGLYGLI
Sbjct: 82   HWHGIRQIGSPWFDGTEGVTQCPVLPGDTFKYQFVVDQAGTYLYHAHYGMQREAGLYGLI 141

Query: 462  RVTPLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIPFQWVGEPQSLLIHGKGRFNCS 641
            RV+P +PEPF++D+DRSIIL+DW+H+STYEQAAGLS+IPFQWVGEPQSLLI+GKGRFNCS
Sbjct: 142  RVSPRQPEPFSYDYDRSIILDDWFHKSTYEQAAGLSAIPFQWVGEPQSLLINGKGRFNCS 201

Query: 642  TTPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVASLTSLSALSFQIEGHNMTVVEADGH 821
             +  I  G+CN++NPEC+PF  TV+PGKTYRLR+ SLT+LSALSFQI+GH M VVEADGH
Sbjct: 202  LSSPI-TGICNATNPECAPFSQTVVPGKTYRLRIGSLTTLSALSFQIQGHKMRVVEADGH 260

Query: 822  YVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVVSRNRTTPAGLAIFNYYXXXXX 1001
            YVEPF V+NLFIYSGETYSVLVKA+Q+P +NYWITSNVVSRNRTTP GLAIFNYY     
Sbjct: 261  YVEPFEVENLFIYSGETYSVLVKADQDPKRNYWITSNVVSRNRTTPPGLAIFNYYPNHPM 320

Query: 1002 XXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKPPTTSDRVIVLLNTQNTINGFRHWS 1181
                       AWD V+PR+AQSLAIKA QG+I+KPPTTSDRVIVLLNTQNTING+R WS
Sbjct: 321  RSPPTPPPTPPAWDDVKPRLAQSLAIKARQGFIQKPPTTSDRVIVLLNTQNTINGYRKWS 380

Query: 1182 VNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDFVNYDIFKVANNSNATTSNGIYRLK 1361
            VNNVSFSLP TPYL+ALK N+  AFDQTPPP+GYD VNYDIF V NN+NAT+SNGIYRL+
Sbjct: 381  VNNVSFSLPQTPYLVALKDNLKDAFDQTPPPEGYDSVNYDIFSVQNNTNATSSNGIYRLE 440

Query: 1362 FNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFDAKNDTKNYNLVNPIMKN 1541
            FN+TVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYG GKFD   D K YNL NPI+KN
Sbjct: 441  FNSTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGNGKFDKDKDPKKYNLENPILKN 500

Query: 1542 TVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEGIDRVG-KLPSSIMGCGK 1718
            TVPVHP+GWTALRF+++NPGVW FHCHI+SHFYMGMGVVFEEGI R+  KLPSSIMGCG+
Sbjct: 501  TVPVHPYGWTALRFKANNPGVWLFHCHIDSHFYMGMGVVFEEGIHRLAEKLPSSIMGCGE 560

Query: 1719 SKGF 1730
            +K F
Sbjct: 561  TKTF 564


>ref|XP_016189927.1| L-ascorbate oxidase isoform X1 [Arachis ipaensis]
          Length = 575

 Score =  926 bits (2393), Expect = 0.0
 Identities = 430/544 (79%), Positives = 485/544 (89%), Gaps = 1/544 (0%)
 Frame = +3

Query: 102  EAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEGDTVVVEVTNKLLTENLAI 281
            E +IRHY+W+VKYEYKS DCFKKLVITVNGR+PGPTIQA+EGDTVVVEV N L+TENLAI
Sbjct: 31   EGRIRHYRWDVKYEYKSPDCFKKLVITVNGRSPGPTIQAEEGDTVVVEVNNSLVTENLAI 90

Query: 282  HWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPGTYLYHAHYGMQREAGLYGLI 461
            HWHGIRQIG+PWFDGTEGV+QCP+LPGD F Y+FVVD+ GTYLYHAHYGMQREAGLYGLI
Sbjct: 91   HWHGIRQIGSPWFDGTEGVTQCPVLPGDTFKYQFVVDQAGTYLYHAHYGMQREAGLYGLI 150

Query: 462  RVTPLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIPFQWVGEPQSLLIHGKGRFNCS 641
            RV+P +PEPF++D+DRSIIL+DW+H+STYEQAAGLS+IPFQWVGEPQSLLI+GKGRFNCS
Sbjct: 151  RVSPRQPEPFSYDYDRSIILDDWFHKSTYEQAAGLSAIPFQWVGEPQSLLINGKGRFNCS 210

Query: 642  TTPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVASLTSLSALSFQIEGHNMTVVEADGH 821
             +  I  G+CN++NPEC+PF  TV+PGKTYRLR+ SLT+LSALSFQI+GH M VVEADGH
Sbjct: 211  LSSPI-TGICNATNPECAPFSQTVVPGKTYRLRIGSLTTLSALSFQIQGHKMRVVEADGH 269

Query: 822  YVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVVSRNRTTPAGLAIFNYYXXXXX 1001
            YVEPF V+NLFIYSGETYSVLVKA+Q+P +NYWITSNVVSRNRTTP GLAIFNYY     
Sbjct: 270  YVEPFEVENLFIYSGETYSVLVKADQDPKRNYWITSNVVSRNRTTPPGLAIFNYYPNHPM 329

Query: 1002 XXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKPPTTSDRVIVLLNTQNTINGFRHWS 1181
                       AWD V+PR+AQSLAIKA QG+I+KPPTTSDRVIVLLNTQNTING+R WS
Sbjct: 330  RSPPTPPPTPPAWDDVKPRLAQSLAIKARQGFIQKPPTTSDRVIVLLNTQNTINGYRKWS 389

Query: 1182 VNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDFVNYDIFKVANNSNATTSNGIYRLK 1361
            VNNVSFSLP TPYL+ALK N+  AFDQTPPP+GYD VNYDIF V NN+NAT+SNGIYRL+
Sbjct: 390  VNNVSFSLPQTPYLVALKDNLKDAFDQTPPPEGYDSVNYDIFSVQNNTNATSSNGIYRLE 449

Query: 1362 FNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFDAKNDTKNYNLVNPIMKN 1541
            FN+TVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYG GKFD   D K YNL NPI+KN
Sbjct: 450  FNSTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGNGKFDKDKDPKKYNLENPILKN 509

Query: 1542 TVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEGIDRVG-KLPSSIMGCGK 1718
            TVPVHP+GWTALRF+++NPGVW FHCHI+SHFYMGMGVVFEEGI R+  KLPSSIMGCG+
Sbjct: 510  TVPVHPYGWTALRFKANNPGVWLFHCHIDSHFYMGMGVVFEEGIHRLAEKLPSSIMGCGE 569

Query: 1719 SKGF 1730
            +K F
Sbjct: 570  TKTF 573


>ref|XP_024194634.1| L-ascorbate oxidase-like [Rosa chinensis]
 gb|PRQ38544.1| putative oxidoreductase [Rosa chinensis]
          Length = 590

 Score =  920 bits (2378), Expect = 0.0
 Identities = 436/571 (76%), Positives = 486/571 (85%), Gaps = 13/571 (2%)
 Frame = +3

Query: 54   LQLSLLVLCFFFILSTEAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEGDT 233
            L L LL  C   I + EA+IRHYKWEVKYEYKS DCFKKLVIT+NG TPGPTI AQ+GDT
Sbjct: 15   LGLCLLFCCLVEIPAVEARIRHYKWEVKYEYKSPDCFKKLVITINGGTPGPTILAQQGDT 74

Query: 234  VVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPGTYLY 413
            VVVE+ N LLTEN+AIHWHGIRQIGTPW DG EGV+QCPILPGD F Y+FVVDRPGTYLY
Sbjct: 75   VVVELKNSLLTENVAIHWHGIRQIGTPWSDGAEGVTQCPILPGDTFKYQFVVDRPGTYLY 134

Query: 414  HAHYGMQREAGLYGLIRVT--PLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIPFQW 587
            HAHYGMQREAGLYG IRV     E EPF +D+DRSIILNDWYH+STYE A GLSS PF W
Sbjct: 135  HAHYGMQREAGLYGSIRVALPEGESEPFLYDYDRSIILNDWYHRSTYEHAVGLSSNPFVW 194

Query: 588  VGEPQSLLIHGKGRFNCST--TPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVASLTSL 761
            VGEPQSLLI GKGRFNCS+  TP+++ GVCN+S+P+CSP+ +TV+PGKTYRLR+ASLT+L
Sbjct: 195  VGEPQSLLIQGKGRFNCSSIATPSLEPGVCNTSSPDCSPYAVTVVPGKTYRLRIASLTAL 254

Query: 762  SALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVVS 941
            SALSFQIEGHN+TVVEADGHYVEPFVV+NLFIYSGETYSVL+KA+Q+PS+NYWIT+N+VS
Sbjct: 255  SALSFQIEGHNLTVVEADGHYVEPFVVKNLFIYSGETYSVLIKADQDPSRNYWITTNIVS 314

Query: 942  RN---------RTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQG 1094
            RN          TTP GLA  NYY                AW+ V+PR+ QSLAIKAH+ 
Sbjct: 315  RNPTTTTQNVTTTTPPGLAFLNYYPNHPRRSPPTVPPVGPAWNDVQPRLNQSLAIKAHRD 374

Query: 1095 YIKKPPTTSDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPP 1274
            +I  PP T DRVIV LNTQN ING R WSVNNVSF+LPHTPYLIAL+ N+  AFDQTPPP
Sbjct: 375  FIHTPPQTPDRVIVFLNTQNQINGNRRWSVNNVSFTLPHTPYLIALQQNLTDAFDQTPPP 434

Query: 1275 DGYDFVNYDIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHD 1454
            +GYDFVNYDI+KVANNSNATTS+GIYRL+FNTTVD+ILQNANTMN NNSETHPWHLHGHD
Sbjct: 435  EGYDFVNYDIYKVANNSNATTSDGIYRLQFNTTVDIILQNANTMNANNSETHPWHLHGHD 494

Query: 1455 FWVLGYGKGKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESH 1634
            FWVLGYG+GKFD  ND K YNLVNPIMKNTVPVHP+GWTALRFR+DNPGVWAFHCHIESH
Sbjct: 495  FWVLGYGEGKFDINNDPKKYNLVNPIMKNTVPVHPYGWTALRFRADNPGVWAFHCHIESH 554

Query: 1635 FYMGMGVVFEEGIDRVGKLPSSIMGCGKSKG 1727
            FYMGMGVVF EGIDR+G LPSSIMGCG++KG
Sbjct: 555  FYMGMGVVFAEGIDRLGNLPSSIMGCGETKG 585


>ref|XP_011097600.1| L-ascorbate oxidase [Sesamum indicum]
          Length = 570

 Score =  918 bits (2373), Expect = 0.0
 Identities = 426/566 (75%), Positives = 486/566 (85%), Gaps = 2/566 (0%)
 Frame = +3

Query: 48   VKLQLSLLVLCFFFILSTEAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQEG 227
            +K    L ++C   +   EA++R YKWEVKYE+KS DC+KKLVIT+NGRTPGP+I AQ+G
Sbjct: 6    IKFLTLLALVCCIVVPLVEARVRLYKWEVKYEFKSPDCYKKLVITINGRTPGPSIVAQQG 65

Query: 228  DTVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPGTY 407
            DT+VVE+ N LLTEN+AIHWHGIRQIGTPW DGTEGV+QCPILPG+ FVY+FVVDRPGTY
Sbjct: 66   DTIVVELKNSLLTENVAIHWHGIRQIGTPWMDGTEGVTQCPILPGETFVYRFVVDRPGTY 125

Query: 408  LYHAHYGMQREAGLYGLIRV--TPLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSIPF 581
            LYHAHYGMQREAGLYG IRV     E EPF++D+DRSIILNDWYH STYEQAAGLSSIPF
Sbjct: 126  LYHAHYGMQREAGLYGSIRVGLPDGETEPFSYDYDRSIILNDWYHNSTYEQAAGLSSIPF 185

Query: 582  QWVGEPQSLLIHGKGRFNCSTTPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVASLTSL 761
             WVGEPQS+LIHG+GRFNC+T P I+AG+CN+++PEC P+ LTV+PGKTYRLR+ SLT+L
Sbjct: 186  VWVGEPQSILIHGRGRFNCNT-PGIEAGLCNATSPECVPYALTVVPGKTYRLRIGSLTAL 244

Query: 762  SALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITSNVVS 941
            SALSF+IEGHNMTVVE DGHYVEPFVV+NLFIYSGETYSVL+KA+Q+PS+NYW ++ VVS
Sbjct: 245  SALSFEIEGHNMTVVETDGHYVEPFVVKNLFIYSGETYSVLIKADQDPSRNYWASAKVVS 304

Query: 942  RNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKPPTTS 1121
            RN TTP GLAIFNYY                 WD V PR++QSLAIKAH GYI  PP TS
Sbjct: 305  RNSTTPNGLAIFNYYPNHPRRNPPTPPPVGPRWDDVGPRLSQSLAIKAHHGYIHPPPQTS 364

Query: 1122 DRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDFVNYD 1301
            DRVIV+LNTQN +NG   WSVNNVSF+LPHTPYLIA KHN+  AF+QTPPPDGYD+ NYD
Sbjct: 365  DRVIVMLNTQNRVNGNVRWSVNNVSFNLPHTPYLIAYKHNLLHAFEQTPPPDGYDYANYD 424

Query: 1302 IFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLGYGKG 1481
            IF VA N NATTSNG+YRL+FNTTVD+ILQNANTMN NNSETHPWHLHGHDFWV+G+G G
Sbjct: 425  IFSVAKNVNATTSNGVYRLRFNTTVDIILQNANTMNPNNSETHPWHLHGHDFWVVGHGTG 484

Query: 1482 KFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVF 1661
            KFD  ND K YNLV+PIMKNTV VHP+GWTALRFR+DNPGVWAFHCHIESHFYMGMGVVF
Sbjct: 485  KFDKFNDPKKYNLVDPIMKNTVAVHPYGWTALRFRADNPGVWAFHCHIESHFYMGMGVVF 544

Query: 1662 EEGIDRVGKLPSSIMGCGKSKGFLRP 1739
            EEG++RVGKLP+SIMGCG SKG+ RP
Sbjct: 545  EEGVERVGKLPTSIMGCGDSKGYHRP 570


>ref|XP_021816393.1| L-ascorbate oxidase-like [Prunus avium]
          Length = 580

 Score =  914 bits (2361), Expect = 0.0
 Identities = 431/570 (75%), Positives = 489/570 (85%), Gaps = 5/570 (0%)
 Frame = +3

Query: 45   MVKLQLSL-LVLCFFFILSTEAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQ 221
            ++KL L+L L L    + + EA+IR+YKWEVKYEYKS DCFKKLV+T+NGR+PGPTI AQ
Sbjct: 11   ILKLFLALFLFLSLVEVPAVEARIRNYKWEVKYEYKSPDCFKKLVLTINGRSPGPTILAQ 70

Query: 222  EGDTVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPG 401
            +GDT++VE+TN L TEN+AIHWHGIRQIGTPW DGTEGV+QCPI+PGD F Y+FVVDRPG
Sbjct: 71   QGDTIIVELTNSLWTENVAIHWHGIRQIGTPWSDGTEGVTQCPIVPGDTFKYQFVVDRPG 130

Query: 402  TYLYHAHYGMQREAGLYGLIRVT--PLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSI 575
            TYLYHAHYGMQREAGLYG IRV     E EPF++D+DRSIILNDWYH+STYE AAGLSS+
Sbjct: 131  TYLYHAHYGMQREAGLYGSIRVALPDGESEPFSYDYDRSIILNDWYHKSTYEHAAGLSSL 190

Query: 576  PFQWVGEPQSLLIHGKGRFNCST--TPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVAS 749
             F WVGEPQSLLI GKGRF+CST  TP++   VCN++NPECSP+ +TV+PGKTYRLRVAS
Sbjct: 191  KFAWVGEPQSLLIQGKGRFDCSTLTTPSLDPDVCNATNPECSPYAMTVVPGKTYRLRVAS 250

Query: 750  LTSLSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITS 929
            LT+LSALSFQIEGHNMTVVEADGHYVEPFVV+NLF+YSGETYSVL+KA+Q+PS+NYW+T+
Sbjct: 251  LTALSALSFQIEGHNMTVVEADGHYVEPFVVKNLFLYSGETYSVLIKADQDPSRNYWMTT 310

Query: 930  NVVSRNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKP 1109
            NVV+RN TTP GLAI NYY                AWD+V  R+ QSLAIKAHQG+I  P
Sbjct: 311  NVVTRNATTPPGLAILNYYPNHPRRSPPAVPPAGPAWDNVRARLDQSLAIKAHQGFIHTP 370

Query: 1110 PTTSDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDF 1289
            P TSDRVIVLLNTQN +NG+  WSVNNVSF+ PHTPYLIALK N+  AFDQT PPDGYDF
Sbjct: 371  PPTSDRVIVLLNTQNKVNGYVRWSVNNVSFTHPHTPYLIALKQNLTEAFDQTSPPDGYDF 430

Query: 1290 VNYDIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLG 1469
            VNYDI+K  N +NAT SNGIYRL+FNTTVD+ILQNANTMN NNSETHPWHLHGHDFWVLG
Sbjct: 431  VNYDIYKTPNYTNATVSNGIYRLQFNTTVDLILQNANTMNPNNSETHPWHLHGHDFWVLG 490

Query: 1470 YGKGKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGM 1649
            YG+GKFD  ND K YNLVNPIMKNTVPVH +GWTALRFR+DNPG WAFHCHIESHFYMGM
Sbjct: 491  YGEGKFDTFNDPKKYNLVNPIMKNTVPVHRYGWTALRFRADNPGAWAFHCHIESHFYMGM 550

Query: 1650 GVVFEEGIDRVGKLPSSIMGCGKSKGFLRP 1739
            GVVFE GID+VGKLPSSIMGCG ++G  RP
Sbjct: 551  GVVFESGIDKVGKLPSSIMGCGATRGVHRP 580


>ref|XP_008233457.2| PREDICTED: L-ascorbate oxidase isoform X1 [Prunus mume]
 ref|XP_016646829.1| PREDICTED: L-ascorbate oxidase isoform X2 [Prunus mume]
 ref|XP_016646830.1| PREDICTED: L-ascorbate oxidase isoform X3 [Prunus mume]
          Length = 580

 Score =  913 bits (2360), Expect = 0.0
 Identities = 430/570 (75%), Positives = 489/570 (85%), Gaps = 5/570 (0%)
 Frame = +3

Query: 45   MVKLQLSL-LVLCFFFILSTEAKIRHYKWEVKYEYKSLDCFKKLVITVNGRTPGPTIQAQ 221
            ++KL L+L L L    + + EA+IRHYKWEVKYEYKS DCFKKLVIT+NGR+PGPTI AQ
Sbjct: 11   ILKLFLALFLFLSLVEVPAVEARIRHYKWEVKYEYKSPDCFKKLVITINGRSPGPTILAQ 70

Query: 222  EGDTVVVEVTNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDIFVYKFVVDRPG 401
            +GDT++VE+TN L TEN+AIHWHGIRQIGTPW DGTEGV+QCPI+PGD F Y+FVVDRPG
Sbjct: 71   QGDTIIVELTNSLWTENVAIHWHGIRQIGTPWSDGTEGVTQCPIVPGDKFKYQFVVDRPG 130

Query: 402  TYLYHAHYGMQREAGLYGLIRVT--PLEPEPFAFDFDRSIILNDWYHQSTYEQAAGLSSI 575
            TYLYHAHYGMQREAGLYG IRV     E EPF++D+DRSIILNDW+H+STYE A GLSS+
Sbjct: 131  TYLYHAHYGMQREAGLYGSIRVALPDGESEPFSYDYDRSIILNDWFHKSTYENAVGLSSL 190

Query: 576  PFQWVGEPQSLLIHGKGRFNCST--TPTIQAGVCNSSNPECSPFLLTVIPGKTYRLRVAS 749
             F WVGEPQSLLI G+GRF+CST  TP++   VCN++NPECSP+ +TV+PGKTYRLRVAS
Sbjct: 191  KFAWVGEPQSLLIQGRGRFDCSTLTTPSLDPEVCNATNPECSPYAMTVVPGKTYRLRVAS 250

Query: 750  LTSLSALSFQIEGHNMTVVEADGHYVEPFVVQNLFIYSGETYSVLVKANQNPSKNYWITS 929
            LTSLSALSFQIEGHNMTVVEADGHYVEPFVV+NLF+YSGETYSVL+KA+Q+PS+NYW+T+
Sbjct: 251  LTSLSALSFQIEGHNMTVVEADGHYVEPFVVKNLFLYSGETYSVLIKADQDPSRNYWMTT 310

Query: 930  NVVSRNRTTPAGLAIFNYYXXXXXXXXXXXXXXXXAWDHVEPRIAQSLAIKAHQGYIKKP 1109
            NVVSRN TTP GLAI NYY                AWD+V  R+ QSLAIK+HQG+I  P
Sbjct: 311  NVVSRNATTPPGLAILNYYPNHPRRSPSAVPPAGPAWDNVRARLDQSLAIKSHQGFIHTP 370

Query: 1110 PTTSDRVIVLLNTQNTINGFRHWSVNNVSFSLPHTPYLIALKHNINGAFDQTPPPDGYDF 1289
            P T+DRVIVLLNTQN +NG+  WSVNNVSF+ PHTPYLIALK N+  AFDQT PPDGYDF
Sbjct: 371  PPTADRVIVLLNTQNKVNGYVRWSVNNVSFTHPHTPYLIALKQNLTDAFDQTSPPDGYDF 430

Query: 1290 VNYDIFKVANNSNATTSNGIYRLKFNTTVDVILQNANTMNKNNSETHPWHLHGHDFWVLG 1469
            VNYDI+K  NN+NAT SNGIYRL+FNTTVD+ILQNANTMN NNSETHPWHLHGHDFWVLG
Sbjct: 431  VNYDIYKTPNNTNATVSNGIYRLQFNTTVDLILQNANTMNPNNSETHPWHLHGHDFWVLG 490

Query: 1470 YGKGKFDAKNDTKNYNLVNPIMKNTVPVHPFGWTALRFRSDNPGVWAFHCHIESHFYMGM 1649
            YG+GKFD  ND K YNLVNPIMKNTVPVH +GWTALRFR+DNPG WAFHCHIESHF+MGM
Sbjct: 491  YGEGKFDIFNDPKKYNLVNPIMKNTVPVHRYGWTALRFRADNPGAWAFHCHIESHFFMGM 550

Query: 1650 GVVFEEGIDRVGKLPSSIMGCGKSKGFLRP 1739
            GVVFE GID+VGKLPSSIMGCG ++G  RP
Sbjct: 551  GVVFESGIDKVGKLPSSIMGCGATRGVHRP 580