BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00018491
         (405 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PNY06815.1| DNA topoisomerase 2-like protein [Trifolium prate...   111   3e-28
sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName:...   111   8e-26
gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium prate...   111   8e-26
ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arieti...   111   1e-25
dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subt...   110   2e-25
dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subt...   110   3e-25
ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago tru...   103   4e-23
ref|XP_022982563.1| DNA topoisomerase 2 isoform X2 [Cucurbita ma...   102   2e-22
ref|XP_022982562.1| DNA topoisomerase 2 isoform X1 [Cucurbita ma...   102   2e-22
ref|XP_022935016.1| DNA topoisomerase 2 isoform X2 [Cucurbita mo...   100   7e-22
ref|XP_022935015.1| DNA topoisomerase 2 isoform X1 [Cucurbita mo...   100   7e-22
ref|XP_023521325.1| DNA topoisomerase 2-like [Cucurbita pepo sub...   100   8e-22
ref|XP_023527826.1| DNA topoisomerase 2 isoform X6 [Cucurbita pe...   100   9e-22
ref|XP_023527825.1| DNA topoisomerase 2 isoform X5 [Cucurbita pe...   100   9e-22
ref|XP_023527823.1| DNA topoisomerase 2 isoform X3 [Cucurbita pe...   100   9e-22
ref|XP_008461811.1| PREDICTED: DNA topoisomerase 2 [Cucumis melo]     100   9e-22
ref|XP_023527820.1| DNA topoisomerase 2 isoform X1 [Cucurbita pe...   100   9e-22
ref|XP_011651769.1| PREDICTED: DNA topoisomerase 2 [Cucumis sati...   100   9e-22
gb|OMO96832.1| DNA topoisomerase, type IIA, partial [Corchorus o...    94   1e-21
dbj|GAU12624.1| hypothetical protein TSUD_121140 [Trifolium subt...    91   2e-20

>gb|PNY06815.1| DNA topoisomerase 2-like protein [Trifolium pratense]
          Length = 192

 Score =  111 bits (278), Expect = 3e-28
 Identities = 58/96 (60%), Positives = 72/96 (75%), Gaps = 3/96 (3%)
 Frame = -1

Query: 279 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 109
           MEK PL+TSNAANI S+ +E   Q+      IL     ++G +++ TQNLWVYEND+MV 
Sbjct: 1   MEKRPLQTSNAANIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVN 60

Query: 108 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVS 1
           RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVT 96


>sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase
           II; AltName: Full=PsTopII
 emb|CAA74891.1| topoisomerase II [Pisum sativum]
          Length = 1462

 Score =  111 bits (278), Expect = 8e-26
 Identities = 56/96 (58%), Positives = 73/96 (76%), Gaps = 3/96 (3%)
 Frame = -1

Query: 279 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 109
           MEK PL+TSNAANI S+ +E   Q+      IL     ++G +++ TQNLWVYEND+MV+
Sbjct: 1   MEKRPLQTSNAANIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVH 60

Query: 108 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVS 1
           R++SYVPGLYKIFDEIL++AA+NK  DP MD LKV+
Sbjct: 61  RAVSYVPGLYKIFDEILVNAADNKQRDPSMDSLKVT 96


>gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium pratense]
          Length = 1492

 Score =  111 bits (278), Expect = 8e-26
 Identities = 58/96 (60%), Positives = 72/96 (75%), Gaps = 3/96 (3%)
 Frame = -1

Query: 279 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 109
           MEK PL+TSNAANI S+ +E   Q+      IL     ++G +++ TQNLWVYEND+MV 
Sbjct: 1   MEKRPLQTSNAANIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVN 60

Query: 108 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVS 1
           RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVT 96


>ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
 ref|XP_004517303.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
          Length = 1472

 Score =  111 bits (277), Expect = 1e-25
 Identities = 56/96 (58%), Positives = 72/96 (75%), Gaps = 3/96 (3%)
 Frame = -1

Query: 279 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 109
           MEK PL+TSNA NI S+ +E   Q+      IL     ++G +++ TQNLW+YEND+MV+
Sbjct: 1   MEKRPLQTSNATNIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYENDEMVH 60

Query: 108 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVS 1
           RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVT 96


>dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subterraneum]
          Length = 1336

 Score =  110 bits (275), Expect = 2e-25
 Identities = 57/96 (59%), Positives = 71/96 (73%), Gaps = 3/96 (3%)
 Frame = -1

Query: 279 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 109
           MEK PL+TSNA NI S+ +E   Q+      IL     ++G +++ TQNLWVYEND+MV 
Sbjct: 1   MEKRPLQTSNATNIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVQ 60

Query: 108 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVS 1
           RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVT 96


>dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subterraneum]
          Length = 1359

 Score =  110 bits (274), Expect = 3e-25
 Identities = 57/96 (59%), Positives = 71/96 (73%), Gaps = 3/96 (3%)
 Frame = -1

Query: 279 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 109
           MEK PL+TSNA NI S+ +E   Q+      IL     ++G +++ TQNLWVYEND+MV 
Sbjct: 1   MEKRPLQTSNATNIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVN 60

Query: 108 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVS 1
           RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVT 96


>ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago truncatula]
 gb|AES69522.2| DNA topoisomerase IV subunit A [Medicago truncatula]
          Length = 1504

 Score =  103 bits (258), Expect = 4e-23
 Identities = 54/96 (56%), Positives = 69/96 (71%), Gaps = 3/96 (3%)
 Frame = -1

Query: 279 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 109
           MEK PL+ S  ANI S+ +E   Q+      IL     ++G +++ TQNLWVYEN++MV 
Sbjct: 1   MEKRPLQPSTTANIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENEEMVN 60

Query: 108 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVS 1
           RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVT 96


>ref|XP_022982563.1| DNA topoisomerase 2 isoform X2 [Cucurbita maxima]
          Length = 1490

 Score =  102 bits (253), Expect = 2e-22
 Identities = 53/101 (52%), Positives = 71/101 (70%), Gaps = 9/101 (8%)
 Frame = -1

Query: 279 MEKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYE 127
           +EK PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLW+YE
Sbjct: 3   VEKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYE 62

Query: 126 NDKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKV 4
           NDKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV
Sbjct: 63  NDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKV 103


>ref|XP_022982562.1| DNA topoisomerase 2 isoform X1 [Cucurbita maxima]
          Length = 1491

 Score =  102 bits (253), Expect = 2e-22
 Identities = 53/101 (52%), Positives = 71/101 (70%), Gaps = 9/101 (8%)
 Frame = -1

Query: 279 MEKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYE 127
           +EK PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLW+YE
Sbjct: 3   VEKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYE 62

Query: 126 NDKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKV 4
           NDKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV
Sbjct: 63  NDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKV 103


>ref|XP_022935016.1| DNA topoisomerase 2 isoform X2 [Cucurbita moschata]
          Length = 1491

 Score =  100 bits (249), Expect = 7e-22
 Identities = 53/100 (53%), Positives = 70/100 (70%), Gaps = 9/100 (9%)
 Frame = -1

Query: 276 EKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYEN 124
           +K PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLWVYEN
Sbjct: 5   KKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN 64

Query: 123 DKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKV 4
           DKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKV 104


>ref|XP_022935015.1| DNA topoisomerase 2 isoform X1 [Cucurbita moschata]
          Length = 1492

 Score =  100 bits (249), Expect = 7e-22
 Identities = 53/100 (53%), Positives = 70/100 (70%), Gaps = 9/100 (9%)
 Frame = -1

Query: 276 EKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYEN 124
           +K PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLWVYEN
Sbjct: 5   KKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN 64

Query: 123 DKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKV 4
           DKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKV 104


>ref|XP_023521325.1| DNA topoisomerase 2-like [Cucurbita pepo subsp. pepo]
          Length = 615

 Score =  100 bits (248), Expect = 8e-22
 Identities = 52/100 (52%), Positives = 70/100 (70%), Gaps = 9/100 (9%)
 Frame = -1

Query: 276 EKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYEN 124
           +K PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLW+YEN
Sbjct: 5   KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 123 DKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKV 4
           DKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKV 104


>ref|XP_023527826.1| DNA topoisomerase 2 isoform X6 [Cucurbita pepo subsp. pepo]
          Length = 1268

 Score =  100 bits (248), Expect = 9e-22
 Identities = 52/100 (52%), Positives = 70/100 (70%), Gaps = 9/100 (9%)
 Frame = -1

Query: 276 EKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYEN 124
           +K PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLW+YEN
Sbjct: 5   KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 123 DKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKV 4
           DKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKV 104


>ref|XP_023527825.1| DNA topoisomerase 2 isoform X5 [Cucurbita pepo subsp. pepo]
          Length = 1269

 Score =  100 bits (248), Expect = 9e-22
 Identities = 52/100 (52%), Positives = 70/100 (70%), Gaps = 9/100 (9%)
 Frame = -1

Query: 276 EKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYEN 124
           +K PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLW+YEN
Sbjct: 5   KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 123 DKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKV 4
           DKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKV 104


>ref|XP_023527823.1| DNA topoisomerase 2 isoform X3 [Cucurbita pepo subsp. pepo]
 ref|XP_023527824.1| DNA topoisomerase 2 isoform X4 [Cucurbita pepo subsp. pepo]
          Length = 1490

 Score =  100 bits (248), Expect = 9e-22
 Identities = 52/100 (52%), Positives = 70/100 (70%), Gaps = 9/100 (9%)
 Frame = -1

Query: 276 EKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYEN 124
           +K PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLW+YEN
Sbjct: 5   KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 123 DKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKV 4
           DKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKV 104


>ref|XP_008461811.1| PREDICTED: DNA topoisomerase 2 [Cucumis melo]
          Length = 1490

 Score =  100 bits (248), Expect = 9e-22
 Identities = 52/101 (51%), Positives = 69/101 (68%), Gaps = 9/101 (8%)
 Frame = -1

Query: 276 EKPPLETSNAANILS---------EKLEASNQRHLDILCFRTMFLGPMDERTQNLWVYEN 124
           +K PL+ SN AN+ S         E++     +   IL     ++G +++ TQNLWVYEN
Sbjct: 5   KKRPLQNSNTANVDSNPAGPKKTIEEMYQKKSQLEHILLRPDTYVGSIEKHTQNLWVYEN 64

Query: 123 DKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVS 1
           DKMV+RSISYVPGLYKIFDEIL++AA+NK  DP MD +KV+
Sbjct: 65  DKMVHRSISYVPGLYKIFDEILVNAADNKQRDPSMDSVKVN 105


>ref|XP_023527820.1| DNA topoisomerase 2 isoform X1 [Cucurbita pepo subsp. pepo]
 ref|XP_023527822.1| DNA topoisomerase 2 isoform X2 [Cucurbita pepo subsp. pepo]
          Length = 1491

 Score =  100 bits (248), Expect = 9e-22
 Identities = 52/100 (52%), Positives = 70/100 (70%), Gaps = 9/100 (9%)
 Frame = -1

Query: 276 EKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYEN 124
           +K PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLW+YEN
Sbjct: 5   KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 123 DKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKV 4
           DKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKV 104


>ref|XP_011651769.1| PREDICTED: DNA topoisomerase 2 [Cucumis sativus]
          Length = 1499

 Score =  100 bits (248), Expect = 9e-22
 Identities = 52/101 (51%), Positives = 70/101 (69%), Gaps = 9/101 (8%)
 Frame = -1

Query: 276 EKPPLETSNAANILS---------EKLEASNQRHLDILCFRTMFLGPMDERTQNLWVYEN 124
           +K PL+ SNAAN+ S         E++     +   IL     ++G +++ TQNLWVYEN
Sbjct: 5   KKRPLQNSNAANVDSNPAGPKKTIEEMYQKKSQLEHILLRPDTYVGSIEKHTQNLWVYEN 64

Query: 123 DKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVS 1
           +KMV+RSISYVPGLYKIFDEIL++AA+NK  DP MD +KV+
Sbjct: 65  EKMVHRSISYVPGLYKIFDEILVNAADNKQRDPSMDSVKVN 105


>gb|OMO96832.1| DNA topoisomerase, type IIA, partial [Corchorus olitorius]
          Length = 184

 Score = 94.4 bits (233), Expect = 1e-21
 Identities = 49/92 (53%), Positives = 65/92 (70%), Gaps = 4/92 (4%)
 Frame = -1

Query: 267 PLETSNAANILSEK-LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVYRSI 100
           PL++SN AN+ S K +E + Q+      IL     ++G +++ TQ LWVYEND+MV R I
Sbjct: 8   PLQSSNIANMKSSKTIEETYQKKSQLEHILLRPDTYIGSIEQHTQRLWVYENDEMVLRDI 67

Query: 99  SYVPGLYKIFDEILLDAAENKHSDPKMDFLKV 4
            YVPGLYKIFDEIL++AA+NK  DP MD +KV
Sbjct: 68  KYVPGLYKIFDEILVNAADNKQRDPSMDCVKV 99


>dbj|GAU12624.1| hypothetical protein TSUD_121140 [Trifolium subterraneum]
          Length = 163

 Score = 90.9 bits (224), Expect = 2e-20
 Identities = 41/58 (70%), Positives = 51/58 (87%)
 Frame = -1

Query: 174 FLGPMDERTQNLWVYENDKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVS 1
           ++G +++ TQNLWVYEND+MV RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+
Sbjct: 18  YVGSIEKHTQNLWVYENDEMVNRSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVT 75