BLASTX nr result
ID: Astragalus23_contig00017480
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Astragalus23_contig00017480
(1365 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006350127.2| PREDICTED: subtilisin-like protease SBT5.3 [... 93 3e-50
ref|XP_021629451.1| subtilisin-like protease SBT5.3 [Manihot esc... 108 3e-50
ref|XP_008224681.1| PREDICTED: subtilisin-like protease SBT5.4 [... 105 4e-50
ref|XP_021828137.1| subtilisin-like protease SBT5.4 [Prunus avium] 104 6e-50
ref|XP_020418597.1| subtilisin-like protease SBT5.4 [Prunus pers... 102 6e-50
ref|XP_008384293.1| PREDICTED: LOW QUALITY PROTEIN: subtilisin-l... 109 8e-50
ref|XP_009374900.1| PREDICTED: LOW QUALITY PROTEIN: subtilisin-l... 107 8e-50
gb|PHT51698.1| Subtilisin-like protease [Capsicum baccatum] 93 2e-49
gb|PHU07606.1| Subtilisin-like protease [Capsicum chinense] 93 2e-49
ref|XP_004251819.1| PREDICTED: subtilisin-like protease SBT5.3 [... 92 2e-49
ref|XP_017979520.1| PREDICTED: subtilisin-like protease SBT5.4 [... 94 4e-49
gb|EOY11902.1| Xylem serine proteinase 1, putative [Theobroma ca... 94 4e-49
gb|PHT72939.1| Subtilisin-like protease [Capsicum annuum] 93 7e-49
ref|XP_016555246.1| PREDICTED: subtilisin-like protease SBT5.3 [... 93 2e-48
ref|XP_013458683.1| subtilisin-like serine protease [Medicago tr... 151 8e-36
ref|XP_003625338.1| subtilisin-like serine protease [Medicago tr... 149 4e-35
ref|XP_017647767.1| PREDICTED: subtilisin-like protease SBT5.4 [... 140 6e-32
ref|XP_004294696.1| PREDICTED: subtilisin-like protease SBT5.3 [... 138 2e-31
gb|PPD96788.1| hypothetical protein GOBAR_DD06194 [Gossypium bar... 137 3e-31
ref|XP_007214967.1| subtilisin-like protease SBT5.3 isoform X2 [... 137 5e-31
>ref|XP_006350127.2| PREDICTED: subtilisin-like protease SBT5.3 [Solanum tuberosum]
Length = 772
Score = 92.8 bits (229), Expect(3) = 3e-50
Identities = 40/56 (71%), Positives = 47/56 (83%)
Frame = -1
Query: 909 FNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPIL 742
F T RD +GHGSHTLST GG+FV+ ANIFG GNGTAK GSPKA++A Y+ CWPPI+
Sbjct: 210 FYTPRDTLGHGSHTLSTAGGNFVEGANIFGYGNGTAKGGSPKARVAAYKACWPPII 265
Score = 92.4 bits (228), Expect(3) = 3e-50
Identities = 48/70 (68%), Positives = 51/70 (72%), Gaps = 1/70 (1%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSS-GFHCNRKLIGARYFYKGYA 947
IIGN+D+GVW ES SF DEGMG PIPSRWKGT T S F CNRKLIGARYF KG+A
Sbjct: 143 IIGNIDSGVWPESESFSDEGMG---PIPSRWKGTCQTGSDPTFRCNRKLIGARYFIKGFA 199
Query: 946 VEAGLDVLKK 917
EAG V K
Sbjct: 200 AEAGALVTSK 209
Score = 65.1 bits (157), Expect(3) = 3e-50
Identities = 27/50 (54%), Positives = 38/50 (76%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H N+VS+FLN G++LHTT SW F+ LEH G P S+ +KAR+G+D I+
Sbjct: 95 KHPNVVSIFLNKGKELHTTRSWNFLGLEHEGKIPKSSLWKKARFGEDIII 144
Score = 90.9 bits (224), Expect = 1e-15
Identities = 47/113 (41%), Positives = 68/113 (60%), Gaps = 3/113 (2%)
Frame = +1
Query: 367 NMFSATPHRCQD-INILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSI 543
+ F+ +C D I+ + N P+I++PK NGS+ +TR LKNVGSP TY A + +G+++
Sbjct: 653 SFFTQGHFKCPDPISFSNLNLPSITVPKLNGSIVVTRTLKNVGSPGTYKAHIRSPIGITV 712
Query: 544 PVQPNILXXXXXXXXXXXXVT--IESTRSSVATVFGRLTWSDGKHYVRSPIVV 696
V+PN L +T ++ ++ VFG L WSD KHYVRSPIVV
Sbjct: 713 VVEPNTLEFKKIGEEKSFKITLKVKGHKAPKDYVFGHLIWSDNKHYVRSPIVV 765
Score = 75.1 bits (183), Expect = 1e-10
Identities = 34/80 (42%), Positives = 49/80 (61%)
Frame = -1
Query: 240 CKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFLP 61
C +GT+DP KAKG ILVC + A VGA+G+++ N GN + +PY P
Sbjct: 402 CIEGTLDPKKAKGTILVCHRGGSPAFNKCTQATSVGAVGIVILNSAFFGNEMYAEPYLCP 461
Query: 60 ASQITYQDGIDVFAYINSTK 1
A+ I+Y DG+ V +Y+NST+
Sbjct: 462 ATHISYSDGLQVSSYVNSTR 481
>ref|XP_021629451.1| subtilisin-like protease SBT5.3 [Manihot esculenta]
gb|OAY35046.1| hypothetical protein MANES_12G067800 [Manihot esculenta]
Length = 770
Score = 88.6 bits (218), Expect(3) = 3e-50
Identities = 38/58 (65%), Positives = 48/58 (82%)
Frame = -1
Query: 918 NIVFNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
N FNT RD GHG+HTLST GG+FV A++FGLGNGTAK GSPK+++A Y+VC+PP+
Sbjct: 209 NSTFNTPRDKEGHGTHTLSTAGGNFVAGASVFGLGNGTAKGGSPKSRVAAYKVCYPPV 266
Score = 88.2 bits (217), Expect(3) = 3e-50
Identities = 45/64 (70%), Positives = 48/64 (75%), Gaps = 1/64 (1%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSS-GFHCNRKLIGARYFYKGYA 947
IIGNLD GVW ES SFRDE +GPI PS+WKG + S GFHCNRKLIGARYF KGYA
Sbjct: 146 IIGNLDTGVWPESKSFRDEVLGPI---PSKWKGICESGSDPGFHCNRKLIGARYFNKGYA 202
Query: 946 VEAG 935
AG
Sbjct: 203 SVAG 206
Score = 73.6 bits (179), Expect(3) = 3e-50
Identities = 34/60 (56%), Positives = 45/60 (75%)
Frame = -1
Query: 1299 EDTIIGNLDGEHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
ED + L +H +VSVFLN G+KLHTTHSW F+ LE NG+ PS+SI +KAR+G+D I+
Sbjct: 89 EDEVAAEL-AKHPKVVSVFLNTGKKLHTTHSWSFLGLEQNGIVPSNSIWKKARFGEDIII 147
Score = 108 bits (271), Expect = 1e-21
Identities = 60/113 (53%), Positives = 76/113 (67%), Gaps = 5/113 (4%)
Frame = +1
Query: 373 FSATPHRC--QDINILDFNYPTISIPKFNGSVTLTRRLKNVGS-PSTYTASLCVLVGLSI 543
FS TP+ C + IN+++FNYP+I+IP FNGSVT+TRR+KNVGS PS+YTA + G +I
Sbjct: 656 FSETPYSCPSKPINLVNFNYPSIAIPDFNGSVTVTRRVKNVGSGPSSYTARVRKPSGFAI 715
Query: 544 PVQPNILXXXXXXXXXXXXVTIESTRSSVAT--VFGRLTWSDGKHYVRSPIVV 696
V+P +L VT +S +SS VFG L WSD KHYVRSPIVV
Sbjct: 716 SVEPKVLKFDKVGEEKSFSVTFKSKKSSARKDFVFGELIWSDNKHYVRSPIVV 768
Score = 94.7 bits (234), Expect = 6e-17
Identities = 44/81 (54%), Positives = 57/81 (70%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LCK GT+DP KAKGKILVCL V A +E A GA+G++L ND++SGN + DP+ L
Sbjct: 402 LCKAGTLDPNKAKGKILVCLRGVNARVEKGEQAALAGAVGMVLANDRDSGNEILADPHVL 461
Query: 63 PASQITYQDGIDVFAYINSTK 1
P S + Y +G+ + AYINSTK
Sbjct: 462 PVSHVNYANGVAILAYINSTK 482
>ref|XP_008224681.1| PREDICTED: subtilisin-like protease SBT5.4 [Prunus mume]
Length = 774
Score = 93.6 bits (231), Expect(3) = 4e-50
Identities = 41/55 (74%), Positives = 48/55 (87%)
Frame = -1
Query: 909 FNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
FN+ARD+ GHGSHTLST G+FV AN+FGLGNGTAK GSPKA++A Y+VCWPPI
Sbjct: 212 FNSARDHEGHGSHTLSTAAGNFVAGANVFGLGNGTAKGGSPKARVAAYKVCWPPI 266
Score = 90.9 bits (224), Expect(3) = 4e-50
Identities = 43/63 (68%), Positives = 49/63 (77%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSSGFHCNRKLIGARYFYKGYAV 944
IIGNLD GVW+ES SF DEG+GPI P++WKG D++GF CNRKLIGARYF KGYA
Sbjct: 146 IIGNLDTGVWAESESFSDEGIGPI---PAKWKGICQNDTAGFPCNRKLIGARYFNKGYAS 202
Query: 943 EAG 935
AG
Sbjct: 203 YAG 205
Score = 65.5 bits (158), Expect(3) = 4e-50
Identities = 32/60 (53%), Positives = 44/60 (73%)
Frame = -1
Query: 1299 EDTIIGNLDGEHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
ED G + +H +VSVFLN GR+LHTTHSW+FM LE +GV S+ ++AR+G+DTI+
Sbjct: 89 EDEEAGEI-AKHPKVVSVFLNQGRQLHTTHSWDFMLLEKDGVIHPTSLWKRARFGEDTII 147
Score = 105 bits (261), Expect = 3e-20
Identities = 55/113 (48%), Positives = 77/113 (68%), Gaps = 4/113 (3%)
Frame = +1
Query: 370 MFSATP-HRC-QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSI 543
+FS +P ++C + ++LDFNYP+I++P+ +GSVT+TRR+KNVGSP TY + +G+S+
Sbjct: 654 LFSESPNYKCPKSASLLDFNYPSIAVPELSGSVTVTRRVKNVGSPGTYAVRIHKPLGVSV 713
Query: 544 PVQPNILXXXXXXXXXXXXVTIESTRSSVAT--VFGRLTWSDGKHYVRSPIVV 696
V+PNIL VT+++ V VFG L WSDGKHYVRSPIVV
Sbjct: 714 TVEPNILKFKNIGEEKSFKVTLKAKGLGVTKGYVFGGLIWSDGKHYVRSPIVV 766
Score = 92.8 bits (229), Expect = 3e-16
Identities = 47/80 (58%), Positives = 54/80 (67%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LC GT+DP K KGKIL CL TA + A GA+G+ILCNDK SGN + DP+ L
Sbjct: 401 LCIGGTLDPQKVKGKILACLRGDTARADKGEQAALAGAVGMILCNDKASGNEIIADPHVL 460
Query: 63 PASQITYQDGIDVFAYINST 4
PASQI Y DGI V +YINST
Sbjct: 461 PASQINYTDGIAVVSYINST 480
>ref|XP_021828137.1| subtilisin-like protease SBT5.4 [Prunus avium]
Length = 774
Score = 93.6 bits (231), Expect(3) = 6e-50
Identities = 41/55 (74%), Positives = 48/55 (87%)
Frame = -1
Query: 909 FNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
FN+ARD+ GHGSHTLST G+FV AN+FGLGNGTAK GSPKA++A Y+VCWPPI
Sbjct: 212 FNSARDHEGHGSHTLSTAAGNFVAGANVFGLGNGTAKGGSPKARVAAYKVCWPPI 266
Score = 90.9 bits (224), Expect(3) = 6e-50
Identities = 43/63 (68%), Positives = 49/63 (77%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSSGFHCNRKLIGARYFYKGYAV 944
IIGNLD GVW+ES SF DEG+GPI P++WKG D++GF CNRKLIGARYF KGYA
Sbjct: 146 IIGNLDTGVWAESESFSDEGIGPI---PAKWKGICQNDTTGFPCNRKLIGARYFNKGYAS 202
Query: 943 EAG 935
AG
Sbjct: 203 YAG 205
Score = 64.7 bits (156), Expect(3) = 6e-50
Identities = 29/50 (58%), Positives = 40/50 (80%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H +VSVFLN GR+LHTTHSW+FM LE +GV S+ ++AR+G+DTI+
Sbjct: 98 KHPKVVSVFLNQGRQLHTTHSWDFMLLEKDGVIHPTSLWKRARFGEDTII 147
Score = 104 bits (260), Expect = 3e-20
Identities = 55/113 (48%), Positives = 77/113 (68%), Gaps = 4/113 (3%)
Frame = +1
Query: 370 MFSATP-HRC-QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSI 543
+FS +P ++C + ++LDFNYP+I++P+ +GSVT+TRR+KNVGSP TY + +G+S+
Sbjct: 654 LFSESPNYKCPKSASLLDFNYPSIAVPELSGSVTVTRRVKNVGSPGTYAVRIHKPLGVSV 713
Query: 544 PVQPNILXXXXXXXXXXXXVTIESTRSSVAT--VFGRLTWSDGKHYVRSPIVV 696
V+PNIL VT+++ V VFG L WSDGKHYVRSPIVV
Sbjct: 714 TVEPNILKFKNIGEEKSFKVTLKAKVLGVTKDYVFGGLIWSDGKHYVRSPIVV 766
Score = 97.1 bits (240), Expect = 1e-17
Identities = 48/80 (60%), Positives = 56/80 (70%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LC GT+DP K KGKIL CL TA ++ A GA+G+ILCNDK SGN + DP+ L
Sbjct: 401 LCIGGTLDPQKVKGKILACLRGDTARVDKGEQAALAGAVGMILCNDKGSGNEIIADPHVL 460
Query: 63 PASQITYQDGIDVFAYINST 4
PASQI Y DGI VF+YINST
Sbjct: 461 PASQINYTDGIAVFSYINST 480
>ref|XP_020418597.1| subtilisin-like protease SBT5.4 [Prunus persica]
gb|ONI09648.1| hypothetical protein PRUPE_4G001800 [Prunus persica]
Length = 774
Score = 93.6 bits (231), Expect(3) = 6e-50
Identities = 41/55 (74%), Positives = 48/55 (87%)
Frame = -1
Query: 909 FNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
FN+ARD+ GHGSHTLST G+FV AN+FGLGNGTAK GSPKA++A Y+VCWPPI
Sbjct: 212 FNSARDHEGHGSHTLSTAAGNFVAGANVFGLGNGTAKGGSPKARVAAYKVCWPPI 266
Score = 90.9 bits (224), Expect(3) = 6e-50
Identities = 43/63 (68%), Positives = 49/63 (77%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSSGFHCNRKLIGARYFYKGYAV 944
IIGNLD GVW+ES SF DEG+GPI P++WKG D++GF CNRKLIGARYF KGYA
Sbjct: 146 IIGNLDTGVWAESESFSDEGIGPI---PAKWKGICQNDTTGFPCNRKLIGARYFNKGYAS 202
Query: 943 EAG 935
AG
Sbjct: 203 YAG 205
Score = 64.7 bits (156), Expect(3) = 6e-50
Identities = 29/50 (58%), Positives = 40/50 (80%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H +VSVFLN GR+LHTTHSW+FM LE +GV S+ ++AR+G+DTI+
Sbjct: 98 KHPKVVSVFLNQGRQLHTTHSWDFMLLEKDGVIHPTSLWKRARFGEDTII 147
Score = 102 bits (255), Expect = 1e-19
Identities = 55/113 (48%), Positives = 75/113 (66%), Gaps = 4/113 (3%)
Frame = +1
Query: 370 MFSATP-HRC-QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSI 543
+FS +P ++C + ++LDFNYP+I +P+ +GSVT+TRR+KNVGSP TY +G+S+
Sbjct: 654 LFSESPNYKCPKSASLLDFNYPSIVVPELSGSVTVTRRVKNVGSPGTYAVRAHKPLGVSV 713
Query: 544 PVQPNILXXXXXXXXXXXXVTIESTRSSVAT--VFGRLTWSDGKHYVRSPIVV 696
V+PNIL VT+++ V VFG L WSDGKHYVRSPIVV
Sbjct: 714 TVEPNILKFKNIGEEKSFKVTLKAKGLGVTKDYVFGGLIWSDGKHYVRSPIVV 766
Score = 93.6 bits (231), Expect = 2e-16
Identities = 47/80 (58%), Positives = 55/80 (68%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LC GT+DP K KGKIL CL TA ++ A GA+G+ILCNDK SGN + DP+ L
Sbjct: 401 LCIGGTLDPQKVKGKILACLRGDTARIDKGEQAALAGAVGMILCNDKASGNEIIADPHVL 460
Query: 63 PASQITYQDGIDVFAYINST 4
PASQI Y DGI V +YINST
Sbjct: 461 PASQINYTDGIAVVSYINST 480
>ref|XP_008384293.1| PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT5.4
[Malus domestica]
Length = 775
Score = 94.0 bits (232), Expect(3) = 8e-50
Identities = 41/54 (75%), Positives = 48/54 (88%)
Frame = -1
Query: 906 NTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
N+ARD+ GHGSHTLST GG+FV AN+FGLGNGTAK GSPKA++A Y+VCWPPI
Sbjct: 213 NSARDHEGHGSHTLSTAGGNFVPGANVFGLGNGTAKGGSPKARVAAYKVCWPPI 266
Score = 87.0 bits (214), Expect(3) = 8e-50
Identities = 41/66 (62%), Positives = 48/66 (72%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSSGFHCNRKLIGARYFYKGYAV 944
IIGNLD GVW ES SF D+G+GP+ PS+WKG ++GF CNRKLIG RYF KGYA
Sbjct: 146 IIGNLDTGVWPESESFXDKGIGPV---PSKWKGICQNHTAGFVCNRKLIGTRYFNKGYAA 202
Query: 943 EAGLDV 926
AG+ V
Sbjct: 203 YAGVPV 208
Score = 67.8 bits (164), Expect(3) = 8e-50
Identities = 31/50 (62%), Positives = 40/50 (80%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H +VSVFLN GRKLHTTHSW+FM LE +GV S+ +KAR+G+DTI+
Sbjct: 98 KHPKVVSVFLNRGRKLHTTHSWDFMLLEKDGVIHPSSLWKKARFGEDTII 147
Score = 109 bits (273), Expect = 8e-22
Identities = 61/123 (49%), Positives = 80/123 (65%), Gaps = 7/123 (5%)
Frame = +1
Query: 349 YQ*CTSNMFSATP-HRC-QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLC 522
Y T +FS P ++C + ++LDFNYP+IS+P+ +GSVT+TRRLKNVGSP TY +
Sbjct: 647 YNKTTIQLFSEGPNYKCPESASLLDFNYPSISVPRLSGSVTVTRRLKNVGSPGTYAVRIK 706
Query: 523 VLVGLSIPVQPNILXXXXXXXXXXXXVTIES--TRSSVAT---VFGRLTWSDGKHYVRSP 687
G+S+ V+PN+L VT+++ R VA VFG LTWSDGKHYVRSP
Sbjct: 707 RANGISVSVEPNVLRFKSVGEEKSFRVTLKAKPKRGGVAKGDYVFGGLTWSDGKHYVRSP 766
Query: 688 IVV 696
IVV
Sbjct: 767 IVV 769
Score = 91.7 bits (226), Expect = 6e-16
Identities = 46/80 (57%), Positives = 55/80 (68%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LC G++DP K KGKIL CL ++A ++ A GA G+ILCND+ SGN L DP+ L
Sbjct: 401 LCLGGSLDPKKVKGKILACLRGLSARVDKGQQASLAGAAGMILCNDEASGNELIADPHVL 460
Query: 63 PASQITYQDGIDVFAYINST 4
PASQI Y DGI VF YINST
Sbjct: 461 PASQINYTDGIAVFNYINST 480
>ref|XP_009374900.1| PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT5.4
[Pyrus x bretschneideri]
Length = 775
Score = 94.0 bits (232), Expect(3) = 8e-50
Identities = 41/54 (75%), Positives = 48/54 (88%)
Frame = -1
Query: 906 NTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
N+ARD+ GHGSHTLST GG+FV AN+FGLGNGTAK GSPKA++A Y+VCWPPI
Sbjct: 213 NSARDHEGHGSHTLSTAGGNFVPGANVFGLGNGTAKGGSPKARVAAYKVCWPPI 266
Score = 87.0 bits (214), Expect(3) = 8e-50
Identities = 41/66 (62%), Positives = 48/66 (72%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSSGFHCNRKLIGARYFYKGYAV 944
IIGNLD GVW ES SF D+G+GP+ PS+WKG ++GF CNRKLIG RYF KGYA
Sbjct: 146 IIGNLDTGVWPESESFSDKGIGPV---PSKWKGICQNHTTGFVCNRKLIGTRYFNKGYAA 202
Query: 943 EAGLDV 926
AG+ V
Sbjct: 203 YAGVPV 208
Score = 67.8 bits (164), Expect(3) = 8e-50
Identities = 31/50 (62%), Positives = 40/50 (80%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H +VSVFLN GRKLHTTHSW+FM LE +GV S+ +KAR+G+DTI+
Sbjct: 98 KHPKVVSVFLNQGRKLHTTHSWDFMLLEKDGVIHPSSLWKKARFGEDTII 147
Score = 107 bits (266), Expect = 6e-21
Identities = 59/123 (47%), Positives = 79/123 (64%), Gaps = 7/123 (5%)
Frame = +1
Query: 349 YQ*CTSNMFSATP-HRC-QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLC 522
Y T +FS P ++C + ++LDFNYP+IS+P+ +GSVT+TRRLKNVG+P TY +
Sbjct: 647 YNKTTIQLFSEGPNYKCPESASLLDFNYPSISVPRLSGSVTVTRRLKNVGAPGTYAVRIK 706
Query: 523 VLVGLSIPVQPNILXXXXXXXXXXXXVTIES--TRSSVAT---VFGRLTWSDGKHYVRSP 687
G+S+ V+PN+L VT+++ R VA VFG LTWSDGKHYVRSP
Sbjct: 707 RANGISVSVEPNVLRFKSVGEEKSFRVTLKAKPKRGGVAKGDYVFGGLTWSDGKHYVRSP 766
Query: 688 IVV 696
VV
Sbjct: 767 FVV 769
Score = 93.2 bits (230), Expect = 2e-16
Identities = 46/80 (57%), Positives = 56/80 (70%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LC G++DP K KGKIL CL ++A ++ A GA+G+ILCND+ SGN L DP+ L
Sbjct: 401 LCLGGSLDPKKVKGKILACLRGLSARVDKGQQASLAGAVGMILCNDEASGNELIADPHVL 460
Query: 63 PASQITYQDGIDVFAYINST 4
PASQI Y DGI VF YINST
Sbjct: 461 PASQINYTDGIAVFNYINST 480
>gb|PHT51698.1| Subtilisin-like protease [Capsicum baccatum]
Length = 1023
Score = 92.8 bits (229), Expect(3) = 2e-49
Identities = 40/56 (71%), Positives = 47/56 (83%)
Frame = -1
Query: 909 FNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPIL 742
F T RD +GHGSHTLST GG+FV+ ANIFG GNGTAK GSPKA++A Y+ CWPPI+
Sbjct: 180 FYTPRDTLGHGSHTLSTAGGNFVEGANIFGYGNGTAKGGSPKARVAAYKACWPPII 235
Score = 92.4 bits (228), Expect(3) = 2e-49
Identities = 48/70 (68%), Positives = 51/70 (72%), Gaps = 1/70 (1%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSS-GFHCNRKLIGARYFYKGYA 947
IIGN+D+GVW ES SF DEGMG PIPSRWKGT T S F CNRKLIGARYF KG+A
Sbjct: 113 IIGNIDSGVWPESESFSDEGMG---PIPSRWKGTCQTGSDPTFRCNRKLIGARYFIKGFA 169
Query: 946 VEAGLDVLKK 917
EAG V K
Sbjct: 170 AEAGALVTSK 179
Score = 62.4 bits (150), Expect(3) = 2e-49
Identities = 27/50 (54%), Positives = 37/50 (74%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H +VSVFLN G++LHTT SW F+ LEH+G P S+ KAR+G+D I+
Sbjct: 65 KHPKVVSVFLNKGKELHTTRSWNFLGLEHDGKIPKGSLWEKARFGEDIII 114
Score = 75.1 bits (183), Expect = 1e-10
Identities = 34/80 (42%), Positives = 51/80 (63%)
Frame = -1
Query: 240 CKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFLP 61
C GT+DP KAKG ILVC +A + A+ VGA+G+++ N GN + +PY P
Sbjct: 372 CIAGTLDPKKAKGAILVCHRGGSAAFDKCTQAILVGAVGIVILNSAFFGNEMYAEPYLCP 431
Query: 60 ASQITYQDGIDVFAYINSTK 1
A+ I+Y DG+ V +Y+N+T+
Sbjct: 432 ATFISYSDGLKVLSYVNATR 451
Score = 66.6 bits (161), Expect = 7e-08
Identities = 36/96 (37%), Positives = 55/96 (57%), Gaps = 2/96 (2%)
Frame = +1
Query: 397 QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSIPVQPNILXXXX 576
+ I+ + N P+I++PK NGSV +TR LKNVGSP TY A + +G+S+ V+PN L
Sbjct: 634 EPISFSNLNLPSITVPKLNGSVVVTRTLKNVGSPGTYKARIRSPIGISVVVEPNTLEFKK 693
Query: 577 XXXXXXXXVTIE--STRSSVATVFGRLTWSDGKHYV 678
+T++ ++ VFG+L W + YV
Sbjct: 694 IGEEKSFKMTLKVIGHKAPKDYVFGQLIWPVLRVYV 729
>gb|PHU07606.1| Subtilisin-like protease [Capsicum chinense]
Length = 806
Score = 92.8 bits (229), Expect(3) = 2e-49
Identities = 40/56 (71%), Positives = 47/56 (83%)
Frame = -1
Query: 909 FNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPIL 742
F T RD +GHGSHTLST GG+FV+ ANIFG GNGTAK GSPKA++A Y+ CWPPI+
Sbjct: 241 FYTPRDTLGHGSHTLSTAGGNFVEGANIFGYGNGTAKGGSPKARVAAYKACWPPII 296
Score = 92.4 bits (228), Expect(3) = 2e-49
Identities = 48/70 (68%), Positives = 51/70 (72%), Gaps = 1/70 (1%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSS-GFHCNRKLIGARYFYKGYA 947
IIGN+D+GVW ES SF DEGMG PIPSRWKGT T S F CNRKLIGARYF KG+A
Sbjct: 174 IIGNIDSGVWPESESFSDEGMG---PIPSRWKGTCQTGSDPTFRCNRKLIGARYFIKGFA 230
Query: 946 VEAGLDVLKK 917
EAG V K
Sbjct: 231 AEAGALVTSK 240
Score = 62.4 bits (150), Expect(3) = 2e-49
Identities = 27/50 (54%), Positives = 37/50 (74%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H +VSVFLN G++LHTT SW F+ LEH+G P S+ KAR+G+D I+
Sbjct: 126 KHPKVVSVFLNKGKELHTTRSWNFLGLEHDGKIPKGSLWEKARFGEDIII 175
Score = 85.1 bits (209), Expect = 9e-14
Identities = 46/103 (44%), Positives = 64/103 (62%), Gaps = 3/103 (2%)
Frame = +1
Query: 397 QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSIPVQPNILXXXX 576
+ I+ + N P+I++PK NGSV +TR LKNVGSP TY A + +G+S+ V+PN L
Sbjct: 695 EPISFSNLNLPSITVPKLNGSVVVTRTLKNVGSPGTYKARIRSPIGISVVVEPNTLEFKK 754
Query: 577 XXXXXXXXVTIE--STRSSVATVFGRLTWSD-GKHYVRSPIVV 696
+T++ ++ VFG+L WSD KHYVRSPIVV
Sbjct: 755 IGEEKSFKMTLKVIGHKAPKDYVFGQLIWSDNNKHYVRSPIVV 797
Score = 75.1 bits (183), Expect = 1e-10
Identities = 34/80 (42%), Positives = 51/80 (63%)
Frame = -1
Query: 240 CKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFLP 61
C GT+DP KAKG ILVC +A + A+ VGA+G+++ N GN + +PY P
Sbjct: 433 CIAGTLDPKKAKGAILVCHRGGSAAFDKCTQAILVGAVGIVILNSAFFGNEMYAEPYLCP 492
Query: 60 ASQITYQDGIDVFAYINSTK 1
A+ I+Y DG+ V +Y+N+T+
Sbjct: 493 ATFISYSDGLKVLSYVNATR 512
>ref|XP_004251819.1| PREDICTED: subtilisin-like protease SBT5.3 [Solanum lycopersicum]
Length = 772
Score = 92.4 bits (228), Expect(3) = 2e-49
Identities = 48/70 (68%), Positives = 51/70 (72%), Gaps = 1/70 (1%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSS-GFHCNRKLIGARYFYKGYA 947
IIGN+D+GVW ES SF DEGMG PIPSRWKGT T S F CNRKLIGARYF KG+A
Sbjct: 143 IIGNIDSGVWPESESFSDEGMG---PIPSRWKGTCQTGSDPTFRCNRKLIGARYFIKGFA 199
Query: 946 VEAGLDVLKK 917
EAG V K
Sbjct: 200 AEAGALVTSK 209
Score = 92.0 bits (227), Expect(3) = 2e-49
Identities = 39/56 (69%), Positives = 48/56 (85%)
Frame = -1
Query: 909 FNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPIL 742
F T RD +GHGSHTLST GG+FV+ ANIFG GNGTAK GSP+A++A+Y+ CWPPI+
Sbjct: 210 FYTPRDTLGHGSHTLSTAGGNFVEGANIFGYGNGTAKGGSPRARVASYKACWPPII 265
Score = 62.8 bits (151), Expect(3) = 2e-49
Identities = 25/50 (50%), Positives = 38/50 (76%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H ++S+FLN G++LHTT SW F+ LEH G P +S+ +KAR+G+D I+
Sbjct: 95 KHPKVISIFLNKGKELHTTRSWNFLGLEHEGKIPKNSLWKKARFGEDIII 144
Score = 87.4 bits (215), Expect = 1e-14
Identities = 45/113 (39%), Positives = 66/113 (58%), Gaps = 3/113 (2%)
Frame = +1
Query: 367 NMFSATPHRCQD-INILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSI 543
+ F+ +C D I+ + N P+I++PK GS+ +TR LKNVG+P TY A + +G+++
Sbjct: 653 SFFTQGHFKCPDPISFSNLNLPSITVPKLKGSIVVTRTLKNVGTPGTYKAHIRSPIGITV 712
Query: 544 PVQPNILXXXXXXXXXXXXVT--IESTRSSVATVFGRLTWSDGKHYVRSPIVV 696
V+PN L +T + ++ VFG L WSD KHYVRSPIVV
Sbjct: 713 VVEPNTLEFRKIGEEKSFKITLKVNGQKAPKDYVFGHLIWSDNKHYVRSPIVV 765
Score = 73.2 bits (178), Expect = 5e-10
Identities = 33/80 (41%), Positives = 51/80 (63%)
Frame = -1
Query: 240 CKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFLP 61
C +GT+DP KAKG ILVC +A + A VGA+G+++ N G+ + +PY P
Sbjct: 402 CIEGTLDPKKAKGTILVCHRGGSAAFSKCIQATSVGAVGIVILNSAFFGDEMYAEPYLCP 461
Query: 60 ASQITYQDGIDVFAYINSTK 1
A+ I+Y DG+ V +Y++ST+
Sbjct: 462 ATFISYSDGLQVSSYVSSTR 481
>ref|XP_017979520.1| PREDICTED: subtilisin-like protease SBT5.4 [Theobroma cacao]
Length = 770
Score = 94.0 bits (232), Expect(3) = 4e-49
Identities = 41/58 (70%), Positives = 46/58 (79%)
Frame = -1
Query: 918 NIVFNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
N TARDN GHG+HTLST GGSFV AN+ G GNGTAK GSPKA++A Y+VCWPPI
Sbjct: 210 NATLETARDNQGHGTHTLSTAGGSFVPGANVLGFGNGTAKGGSPKARVAAYKVCWPPI 267
Score = 87.0 bits (214), Expect(3) = 4e-49
Identities = 43/60 (71%), Positives = 45/60 (75%), Gaps = 1/60 (1%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTD-SSGFHCNRKLIGARYFYKGYA 947
IIGNLD GVW ES SF DEGMG PIPSRWKG+ D G HCNRKLIGA+YF KGYA
Sbjct: 146 IIGNLDTGVWPESKSFSDEGMG---PIPSRWKGSCQNDPDDGVHCNRKLIGAKYFNKGYA 202
Score = 65.5 bits (158), Expect(3) = 4e-49
Identities = 30/50 (60%), Positives = 40/50 (80%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H +VSVFLN GRKLHTT SW+F+ LE++GV S SI +K+R+G DTI+
Sbjct: 98 KHPEVVSVFLNKGRKLHTTRSWDFLGLENDGVIHSSSIWKKSRFGGDTII 147
Score = 94.0 bits (232), Expect = 1e-16
Identities = 45/81 (55%), Positives = 58/81 (71%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LC+ GT+DP K KGKI+VC+ V A + S AL GA+G+IL ND+ SGN + DP+ L
Sbjct: 403 LCQPGTLDPKKVKGKIIVCVRGVNARTDKSQQALLAGAVGMILANDEKSGNGIMADPHLL 462
Query: 63 PASQITYQDGIDVFAYINSTK 1
PA+ I++ DG VFAYINSTK
Sbjct: 463 PATHISFTDGETVFAYINSTK 483
Score = 92.8 bits (229), Expect = 3e-16
Identities = 48/112 (42%), Positives = 68/112 (60%), Gaps = 3/112 (2%)
Frame = +1
Query: 370 MFSATPHRC-QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSIP 546
+FS P+ C + ++ DFNYP+IS+ KF GS T++R++KNVGSP TY A + G+++
Sbjct: 656 LFSDKPYTCPKSFSLSDFNYPSISVDKFGGSATVSRKVKNVGSPGTYRARVRSPAGVTVS 715
Query: 547 VQPNILXXXXXXXXXXXXVTI--ESTRSSVATVFGRLTWSDGKHYVRSPIVV 696
V P+ L VT +S VFG+L WS+G HYVRSP+VV
Sbjct: 716 VNPSTLTFEKKGEEKKFEVTFKAKSNGQPAGFVFGQLIWSNGHHYVRSPLVV 767
>gb|EOY11902.1| Xylem serine proteinase 1, putative [Theobroma cacao]
Length = 770
Score = 94.0 bits (232), Expect(3) = 4e-49
Identities = 41/58 (70%), Positives = 46/58 (79%)
Frame = -1
Query: 918 NIVFNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
N TARDN GHG+HTLST GGSFV AN+ G GNGTAK GSPKA++A Y+VCWPPI
Sbjct: 210 NATLETARDNQGHGTHTLSTAGGSFVPGANVLGFGNGTAKGGSPKARVAAYKVCWPPI 267
Score = 87.0 bits (214), Expect(3) = 4e-49
Identities = 43/60 (71%), Positives = 45/60 (75%), Gaps = 1/60 (1%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTD-SSGFHCNRKLIGARYFYKGYA 947
IIGNLD GVW ES SF DEGMG PIPSRWKG+ D G HCNRKLIGA+YF KGYA
Sbjct: 146 IIGNLDTGVWPESKSFSDEGMG---PIPSRWKGSCQNDPDDGVHCNRKLIGAKYFNKGYA 202
Score = 65.5 bits (158), Expect(3) = 4e-49
Identities = 30/50 (60%), Positives = 40/50 (80%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H +VSVFLN GRKLHTT SW+F+ LE++GV S SI +K+R+G DTI+
Sbjct: 98 KHPEVVSVFLNKGRKLHTTRSWDFLGLENDGVIHSSSIWKKSRFGGDTII 147
Score = 94.0 bits (232), Expect = 1e-16
Identities = 45/81 (55%), Positives = 58/81 (71%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LC+ GT+DP K KGKI+VC+ V A + S AL GA+G+IL ND+ SGN + DP+ L
Sbjct: 403 LCQPGTLDPKKVKGKIIVCVRGVNARTDKSQQALLAGAVGMILANDEKSGNGIMADPHLL 462
Query: 63 PASQITYQDGIDVFAYINSTK 1
PA+ I++ DG VFAYINSTK
Sbjct: 463 PATHISFTDGETVFAYINSTK 483
Score = 92.8 bits (229), Expect = 3e-16
Identities = 48/112 (42%), Positives = 68/112 (60%), Gaps = 3/112 (2%)
Frame = +1
Query: 370 MFSATPHRC-QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSIP 546
+FS P+ C + ++ DFNYP+IS+ KF GS T++R++KNVGSP TY A + G+++
Sbjct: 656 LFSDKPYTCPKSFSLSDFNYPSISVDKFGGSATVSRKVKNVGSPGTYRARVRSPAGVTVS 715
Query: 547 VQPNILXXXXXXXXXXXXVTI--ESTRSSVATVFGRLTWSDGKHYVRSPIVV 696
V P+ L VT +S VFG+L WS+G HYVRSP+VV
Sbjct: 716 VNPSTLTFEKKGEEKKFEVTFKAKSNGQPAGFVFGQLIWSNGHHYVRSPLVV 767
>gb|PHT72939.1| Subtilisin-like protease [Capsicum annuum]
Length = 806
Score = 92.8 bits (229), Expect(3) = 7e-49
Identities = 40/56 (71%), Positives = 47/56 (83%)
Frame = -1
Query: 909 FNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPIL 742
F T RD +GHGSHTLST GG+FV+ ANIFG GNGTAK GSPKA++A Y+ CWPPI+
Sbjct: 241 FYTPRDTLGHGSHTLSTAGGNFVEGANIFGYGNGTAKGGSPKARVAAYKACWPPII 296
Score = 92.4 bits (228), Expect(3) = 7e-49
Identities = 48/70 (68%), Positives = 51/70 (72%), Gaps = 1/70 (1%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSS-GFHCNRKLIGARYFYKGYA 947
IIGN+D+GVW ES SF DEGMG PIPSRWKGT T S F CNRKLIGARYF KG+A
Sbjct: 174 IIGNIDSGVWPESESFSDEGMG---PIPSRWKGTCQTGSDPTFRCNRKLIGARYFIKGFA 230
Query: 946 VEAGLDVLKK 917
EAG V K
Sbjct: 231 AEAGALVTSK 240
Score = 60.5 bits (145), Expect(3) = 7e-49
Identities = 26/50 (52%), Positives = 37/50 (74%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H +VSVFLN G++LHTT SW F+ LEH+G P S+ KAR+G++ I+
Sbjct: 126 KHPKVVSVFLNKGKELHTTRSWNFLGLEHDGKIPKGSLWEKARFGENIII 175
Score = 85.1 bits (209), Expect = 9e-14
Identities = 46/103 (44%), Positives = 64/103 (62%), Gaps = 3/103 (2%)
Frame = +1
Query: 397 QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSIPVQPNILXXXX 576
+ I+ + N P+I++PK NGSV +TR LKNVGSP TY A + +G+S+ V+PN L
Sbjct: 695 EPISFSNLNLPSITVPKLNGSVVVTRTLKNVGSPGTYKARIRPPIGISVVVEPNTLEFKK 754
Query: 577 XXXXXXXXVTIE--STRSSVATVFGRLTWSD-GKHYVRSPIVV 696
+T++ ++ VFG+L WSD KHYVRSPIVV
Sbjct: 755 IGEEKSFKMTLKVIGHKAPKDYVFGQLIWSDNNKHYVRSPIVV 797
Score = 73.2 bits (178), Expect = 6e-10
Identities = 33/80 (41%), Positives = 51/80 (63%)
Frame = -1
Query: 240 CKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFLP 61
C GT+DP KAKG ILVC +A + A+ VGA+G+++ N GN + +PY P
Sbjct: 433 CIAGTLDPKKAKGAILVCHRGGSAAFDKCTQAILVGAVGIVILNSAFFGNEMYAEPYLCP 492
Query: 60 ASQITYQDGIDVFAYINSTK 1
A+ I+Y DG+ V +Y+++T+
Sbjct: 493 ATFISYSDGLKVLSYVHATR 512
>ref|XP_016555246.1| PREDICTED: subtilisin-like protease SBT5.3 [Capsicum annuum]
Length = 806
Score = 92.8 bits (229), Expect(3) = 2e-48
Identities = 40/56 (71%), Positives = 47/56 (83%)
Frame = -1
Query: 909 FNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPIL 742
F T RD +GHGSHTLST GG+FV+ ANIFG GNGTAK GSPKA++A Y+ CWPPI+
Sbjct: 241 FYTPRDTLGHGSHTLSTAGGNFVEGANIFGYGNGTAKGGSPKARVAAYKACWPPII 296
Score = 90.9 bits (224), Expect(3) = 2e-48
Identities = 47/70 (67%), Positives = 51/70 (72%), Gaps = 1/70 (1%)
Frame = -3
Query: 1123 IIGNLDNGVWSESPSFRDEGMGPIIPIPSRWKGTYHTDSSG-FHCNRKLIGARYFYKGYA 947
IIGN+D+GVW ES SF D+GMGPI PSRWKGT T S F CNRKLIGARYF KG+A
Sbjct: 174 IIGNIDSGVWPESESFSDKGMGPI---PSRWKGTCQTGSDPTFRCNRKLIGARYFIKGFA 230
Query: 946 VEAGLDVLKK 917
EAG V K
Sbjct: 231 AEAGALVTSK 240
Score = 60.5 bits (145), Expect(3) = 2e-48
Identities = 26/50 (52%), Positives = 37/50 (74%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTIL 1120
+H +VSVFLN G++LHTT SW F+ LEH+G P S+ KAR+G++ I+
Sbjct: 126 KHPKVVSVFLNKGKELHTTRSWNFLGLEHDGKIPKGSLWEKARFGENIII 175
Score = 85.1 bits (209), Expect = 9e-14
Identities = 46/103 (44%), Positives = 64/103 (62%), Gaps = 3/103 (2%)
Frame = +1
Query: 397 QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSIPVQPNILXXXX 576
+ I+ + N P+I++PK NGSV +TR LKNVGSP TY A + +G+S+ V+PN L
Sbjct: 695 EPISFSNLNLPSITVPKLNGSVVVTRTLKNVGSPGTYKARIRSPIGISVVVEPNTLEFKK 754
Query: 577 XXXXXXXXVTIE--STRSSVATVFGRLTWSD-GKHYVRSPIVV 696
+T++ ++ VFG+L WSD KHYVRSPIVV
Sbjct: 755 IGEEKSFKMTLKVIGHKAPKDYVFGQLIWSDNNKHYVRSPIVV 797
Score = 75.1 bits (183), Expect = 1e-10
Identities = 34/80 (42%), Positives = 51/80 (63%)
Frame = -1
Query: 240 CKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFLP 61
C GT+DP KAKG ILVC +A + A+ VGA+G+++ N GN + +PY P
Sbjct: 433 CIAGTLDPKKAKGAILVCHRGGSAAFDKCTQAILVGAVGIVILNSAFFGNEMYAEPYLCP 492
Query: 60 ASQITYQDGIDVFAYINSTK 1
A+ I+Y DG+ V +Y+N+T+
Sbjct: 493 ATFISYSDGLKVLSYVNATR 512
>ref|XP_013458683.1| subtilisin-like serine protease [Medicago truncatula]
gb|KEH32715.1| subtilisin-like serine protease [Medicago truncatula]
Length = 770
Score = 151 bits (381), Expect = 8e-36
Identities = 87/176 (49%), Positives = 105/176 (59%), Gaps = 1/176 (0%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTILLGILTMVSGQ 1090
EH N+VSV LN GRKLHTTHSWEFMSLEHNGV PS S+L KARYG+D I+ + T V +
Sbjct: 98 EHPNVVSVLLNRGRKLHTTHSWEFMSLEHNGVAPSHSLLTKARYGEDVIIGNLDTGVWPE 157
Query: 1089 NLRALEMREWDP*YPFLQGGKXXXXXXXXXXXXXXX*LEQGTSTK-VMLWKRG*TY*KNI 913
+ + + P K + K + G +N
Sbjct: 158 SPSFRD----EGIGPIPSRWKGTCQSDHTGFRCNRKLIGARYFNKGYAAYAAGSMVLQNG 213
Query: 912 VFNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
+TARDN GHGSHTLSTIGG+FV N+FGLGNGTAK GSPKA++A Y+VCWP I
Sbjct: 214 TLDTARDNEGHGSHTLSTIGGNFVSGVNVFGLGNGTAKGGSPKARVAAYKVCWPAI 269
Score = 150 bits (380), Expect = 1e-35
Identities = 77/112 (68%), Positives = 84/112 (75%)
Frame = +1
Query: 370 MFSATPHRCQDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSIPV 549
MFS TP+ C+DINILDFNYPTI+IPK GSVTL+R+LKNVG P TYTASL V VGLSI V
Sbjct: 657 MFSGTPYHCEDINILDFNYPTITIPKLYGSVTLSRKLKNVGPPGTYTASLRVPVGLSISV 716
Query: 550 QPNILXXXXXXXXXXXXVTIESTRSSVATVFGRLTWSDGKHYVRSPIVVGGV 705
QP L +TIE TRS +ATVFG LTWSDG HYVRSPI VGGV
Sbjct: 717 QPKKLKFDKIGEEKSFSLTIEVTRSGMATVFGGLTWSDGTHYVRSPIAVGGV 768
Score = 114 bits (285), Expect = 2e-23
Identities = 53/82 (64%), Positives = 67/82 (81%)
Frame = -1
Query: 246 TLCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYF 67
TLC GTIDP KA+G+ILVCL ++A +E S VALE A+G+ILCND+++GN L DP+F
Sbjct: 403 TLCMNGTIDPEKARGRILVCLRGISARVEKSFVALEAEAVGMILCNDQSNGNELIADPHF 462
Query: 66 LPASQITYQDGIDVFAYINSTK 1
LPA+QITY DG+ +FAYINSTK
Sbjct: 463 LPATQITYADGVALFAYINSTK 484
>ref|XP_003625338.1| subtilisin-like serine protease [Medicago truncatula]
gb|AES81556.1| subtilisin-like serine protease [Medicago truncatula]
Length = 766
Score = 149 bits (376), Expect = 4e-35
Identities = 84/175 (48%), Positives = 103/175 (58%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTILLGILTMVSGQ 1090
EH N+VSV LN GRKLHTTHSWEFMS+EHNGV PS S+ RKARYG+D I+ + + V +
Sbjct: 95 EHPNVVSVLLNRGRKLHTTHSWEFMSMEHNGVAPSHSLFRKARYGEDVIIGNLDSGVWPE 154
Query: 1089 NLRALEMREWDP*YPFLQGGKXXXXXXXXXXXXXXX*LEQGTSTKVMLWKRG*TY*KNIV 910
+ + + P K + K G +N
Sbjct: 155 SPSFGD----EGIGPIPSRWKGTCQNDHTGFRCNRKLIGARYFNKGYATYAGSEVVQNGT 210
Query: 909 FNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
+T RDN GHGSHTLST+GG+FV AN GLGNGTAK GSPKA++A Y+VCWPPI
Sbjct: 211 LDTPRDNKGHGSHTLSTLGGNFVSGANFVGLGNGTAKGGSPKARVAAYKVCWPPI 265
Score = 140 bits (354), Expect = 3e-32
Identities = 74/113 (65%), Positives = 80/113 (70%)
Frame = +1
Query: 367 NMFSATPHRCQDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSIP 546
+MFS T H C INILDFNYPTI+IP GSVTL+R+LKNVG P TYTASL V GLSI
Sbjct: 652 SMFSGTNHHCDGINILDFNYPTITIPILYGSVTLSRKLKNVGPPGTYTASLRVPAGLSIS 711
Query: 547 VQPNILXXXXXXXXXXXXVTIESTRSSVATVFGRLTWSDGKHYVRSPIVVGGV 705
VQP L +TIE TRS ATVFG LTWSDGKH+VRSPI VGGV
Sbjct: 712 VQPKKLKFDKIGEEKSFNLTIEVTRSGGATVFGGLTWSDGKHHVRSPITVGGV 764
Score = 107 bits (267), Expect = 5e-21
Identities = 51/82 (62%), Positives = 62/82 (75%)
Frame = -1
Query: 246 TLCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYF 67
TLC GTIDP KA G+ILVCL + +E SLVALE A+G+IL ND++ GN LT DP+F
Sbjct: 399 TLCMNGTIDPEKASGRILVCLRGINGKVEKSLVALEAKAVGMILFNDRSHGNELTDDPHF 458
Query: 66 LPASQITYQDGIDVFAYINSTK 1
LP + I Y+DG+ VFAYINSTK
Sbjct: 459 LPTAHIIYEDGVAVFAYINSTK 480
Score = 59.7 bits (143), Expect = 1e-05
Identities = 27/31 (87%), Positives = 28/31 (90%)
Frame = -1
Query: 1365 FMSLEHNGAAPSDSLFRKARYGEDTIIGNLD 1273
FMS+EHNG APS SLFRKARYGED IIGNLD
Sbjct: 118 FMSMEHNGVAPSHSLFRKARYGEDVIIGNLD 148
>ref|XP_017647767.1| PREDICTED: subtilisin-like protease SBT5.4 [Gossypium arboreum]
Length = 789
Score = 140 bits (352), Expect = 6e-32
Identities = 80/182 (43%), Positives = 104/182 (57%), Gaps = 7/182 (3%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTILLGILTMV--- 1099
+H N+VSVFLN GRKLHTT SW+F+ LE NGV PSDS+ +KA++G+DTI+ + T V
Sbjct: 113 KHPNVVSVFLNKGRKLHTTRSWDFLRLERNGVIPSDSLWKKAKFGEDTIIGNLDTGVWPE 172
Query: 1098 ----SGQNLRALEMREWDP*YPFLQGGKXXXXXXXXXXXXXXX*LEQGTSTKVMLWKRG* 931
S + + R W G+ E K+
Sbjct: 173 SKSFSDDGIGPIPSR-WRGSCQRGVDGRFSCNRKLIGAKYFNRGYEAYLGEKL------- 224
Query: 930 TY*KNIVFNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWP 751
N F T RD+ GHGSHTLST GG+FV+ A++FG GNGTAK GSPKA++A Y+VCWP
Sbjct: 225 ----NATFKTVRDHEGHGSHTLSTAGGNFVNGASVFGYGNGTAKGGSPKARVAAYKVCWP 280
Query: 750 PI 745
P+
Sbjct: 281 PV 282
Score = 94.4 bits (233), Expect = 9e-17
Identities = 43/81 (53%), Positives = 57/81 (70%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LC+ G++DP+K KGKILVCL + + AL GA+G+IL ND+ SGN + DP+ L
Sbjct: 418 LCQPGSLDPIKVKGKILVCLRGINPRTDKGKQALLSGAVGMILANDEKSGNEVIADPHLL 477
Query: 63 PASQITYQDGIDVFAYINSTK 1
PA+ + Y DG+ VFAYINSTK
Sbjct: 478 PATHLNYTDGVTVFAYINSTK 498
Score = 87.4 bits (215), Expect = 2e-14
Identities = 47/123 (38%), Positives = 66/123 (53%), Gaps = 7/123 (5%)
Frame = +1
Query: 349 YQ*CTSNMFSATPHRC-QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCV 525
Y T +FS P+ C + N+ D NYP+I++P+ NG+ +TR++KNVG+P TY A +
Sbjct: 664 YNQTTIQLFSNKPYVCPKSHNVADLNYPSITVPELNGTTIITRKVKNVGTPGTYKAHVRS 723
Query: 526 LVGLSIPVQPNILXXXXXXXXXXXXVTIE------STRSSVATVFGRLTWSDGKHYVRSP 687
VG+ + V P+ L V + V VFG L W DG HYVRSP
Sbjct: 724 PVGVMVTVHPSTLKFTKIGEEKKFEVRLRPSSKKVDAEGGVGYVFGVLRWCDGYHYVRSP 783
Query: 688 IVV 696
+VV
Sbjct: 784 LVV 786
>ref|XP_004294696.1| PREDICTED: subtilisin-like protease SBT5.3 [Fragaria vesca subsp.
vesca]
Length = 771
Score = 138 bits (348), Expect = 2e-31
Identities = 77/178 (43%), Positives = 103/178 (57%), Gaps = 3/178 (1%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTILLGILTMVSGQ 1090
+H ++SVFLN GRKLHTT SW+F+ LEHNGV P +S+ KARYG+DTI+ + T V +
Sbjct: 99 KHPKVISVFLNKGRKLHTTRSWDFLELEHNGVTPPNSVWEKARYGEDTIIANLDTGVWPE 158
Query: 1089 NLRALEMREWDP*YPFLQG---GKXXXXXXXXXXXXXXX*LEQGTSTKVMLWKRG*TY*K 919
+ ++ + P P G + +G + V
Sbjct: 159 S-KSFSDEGYGPIPPKWTGICQNETDSGFHCNRKLIGARYFNKGFAAVVGSL-------- 209
Query: 918 NIVFNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
N F + RD GHGSHTLST GG+FV A++FG GNGTAK GSPKA++A Y+VCWPP+
Sbjct: 210 NSSFQSPRDEEGHGSHTLSTAGGNFVTGASVFGYGNGTAKGGSPKARVAAYKVCWPPV 267
Score = 89.4 bits (220), Expect = 4e-15
Identities = 48/112 (42%), Positives = 67/112 (59%), Gaps = 4/112 (3%)
Frame = +1
Query: 373 FSATPHRCQD--INILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSIP 546
FS ++C I++ + NYP+I++PK +GS+ + R LKNVG P TYTA + G+ +
Sbjct: 657 FSQESYKCPSPAISLTNLNYPSITVPKLSGSLVVKRTLKNVGEPGTYTARITNPDGILVS 716
Query: 547 VQPNILXXXXXXXXXXXXVTIESTRSSVAT--VFGRLTWSDGKHYVRSPIVV 696
V+P L V +E+ S+ A VFG+L WSDGKH VRSPIVV
Sbjct: 717 VEPKSLKFKKVGEEKSFNVVLEAKDSNAAKNYVFGKLIWSDGKHNVRSPIVV 768
Score = 84.3 bits (207), Expect = 1e-13
Identities = 41/80 (51%), Positives = 53/80 (66%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LCK GT+DP K KGKIL CL A ++ +L GA+G+IL ND+ +GN + DP+ L
Sbjct: 403 LCKAGTLDPEKVKGKILACLRGDNARVDKGEQSLLAGAVGMILANDEVNGNEIISDPHVL 462
Query: 63 PASQITYQDGIDVFAYINST 4
PA+ I + DG VFAYIN T
Sbjct: 463 PAAHINFTDGALVFAYINET 482
>gb|PPD96788.1| hypothetical protein GOBAR_DD06194 [Gossypium barbadense]
Length = 781
Score = 137 bits (346), Expect = 3e-31
Identities = 78/176 (44%), Positives = 103/176 (58%), Gaps = 1/176 (0%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTILLGILTMVSGQ 1090
+H N+VSVFLN GRKLHTT SW+F+ LE NGV PSDS+ +KA++G+DT ++G L
Sbjct: 105 KHPNVVSVFLNKGRKLHTTRSWDFLRLERNGVIPSDSLWKKAKFGEDT-IIGNLDTGVWP 163
Query: 1089 NLRALEMREWDP*YPFLQGGKXXXXXXXXXXXXXXX*LEQ-GTSTKVMLWKRG*TY*KNI 913
++ P +G + K L ++ N
Sbjct: 164 ESKSFSDDGIGPIPSRWRGSCQRGIDDRFRCNRKLIGAKYFNRGYKAYLGEK-----LNA 218
Query: 912 VFNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
F T RD+ GHGSHTLST GG+FVD A++FG GNGTAK GSPKA++A Y+VCWPP+
Sbjct: 219 TFKTVRDHEGHGSHTLSTAGGNFVDGASVFGYGNGTAKGGSPKARVAAYKVCWPPV 274
Score = 94.4 bits (233), Expect = 9e-17
Identities = 43/81 (53%), Positives = 57/81 (70%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LC+ G++DP K KGKILVCL + A + AL GA+G+IL ND+ SGN + DP+ L
Sbjct: 410 LCQPGSLDPTKVKGKILVCLRGINARTDKGKQALLAGAVGMILANDEKSGNEVIADPHLL 469
Query: 63 PASQITYQDGIDVFAYINSTK 1
PA+ + + DG+ VFAYINSTK
Sbjct: 470 PATHLNFTDGVTVFAYINSTK 490
Score = 89.4 bits (220), Expect = 4e-15
Identities = 47/123 (38%), Positives = 68/123 (55%), Gaps = 7/123 (5%)
Frame = +1
Query: 349 YQ*CTSNMFSATPHRC-QDINILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCV 525
Y T +FS P+ C + N+ D NYP+I++P+ NG+ +TR++KNVG+P TY A +
Sbjct: 656 YNQTTIQLFSNKPYVCPKSYNVADLNYPSITVPELNGTTIITRKVKNVGTPGTYKAHVRS 715
Query: 526 LVGLSIPVQPNILXXXXXXXXXXXXVTIE------STRSSVATVFGRLTWSDGKHYVRSP 687
VG+ + V P+ L V ++ + V VFG L W DG HYVRSP
Sbjct: 716 PVGVMVTVHPSTLKFTKIGEEKKFEVRLKPSSKKVDAKGGVGYVFGVLRWCDGYHYVRSP 775
Query: 688 IVV 696
+VV
Sbjct: 776 LVV 778
>ref|XP_007214967.1| subtilisin-like protease SBT5.3 isoform X2 [Prunus persica]
gb|ONI17229.1| hypothetical protein PRUPE_3G147300 [Prunus persica]
Length = 773
Score = 137 bits (345), Expect = 5e-31
Identities = 76/178 (42%), Positives = 103/178 (57%), Gaps = 3/178 (1%)
Frame = -1
Query: 1269 EHLNIVSVFLN*GRKLHTTHSWEFMSLEHNGVGPSDSILRKARYGKDTILLGILTMVSGQ 1090
+H +VS+FLN GRKLHTT SW+F+ L+H+GV P +SI KARYG+DTI+ G L +
Sbjct: 101 KHPKVVSIFLNQGRKLHTTRSWDFLGLQHDGVTPPNSIWNKARYGEDTII-GNLDSGAWP 159
Query: 1089 NLRALEMREWDP*YPFLQG---GKXXXXXXXXXXXXXXX*LEQGTSTKVMLWKRG*TY*K 919
+ E+ P +G + +G +
Sbjct: 160 ESNSFSDEEYGPIPSKWKGICQNETDSEFYCNRKLIGARYFNKGYAAVAGTL-------- 211
Query: 918 NIVFNTARDNMGHGSHTLSTIGGSFVDRANIFGLGNGTAKCGSPKAKLATYRVCWPPI 745
N F++ RDN+GHGSHTLST GG+FV A++FG GNGTAK GSPKA++A Y+VCWPP+
Sbjct: 212 NSSFDSPRDNVGHGSHTLSTAGGNFVTGASVFGFGNGTAKGGSPKARVAAYKVCWPPV 269
Score = 87.8 bits (216), Expect = 1e-14
Identities = 45/81 (55%), Positives = 55/81 (67%)
Frame = -1
Query: 243 LCKKGTIDPVKAKGKILVCLGDVTAGMENSLVALEVGAIGVILCNDKNSGNVLTPDPYFL 64
LCK GT+D K KGKIL CL +A ++ AL GA+G+IL ND+ SGN + DP+ L
Sbjct: 405 LCKAGTLDLKKVKGKILACLRGDSARVDKGEQALLAGAVGMILANDELSGNEIISDPHVL 464
Query: 63 PASQITYQDGIDVFAYINSTK 1
PAS I + DG VFAYINSTK
Sbjct: 465 PASHINFTDGALVFAYINSTK 485
Score = 84.3 bits (207), Expect = 1e-13
Identities = 46/113 (40%), Positives = 67/113 (59%), Gaps = 4/113 (3%)
Frame = +1
Query: 370 MFSATPHRCQD--INILDFNYPTISIPKFNGSVTLTRRLKNVGSPSTYTASLCVLVGLSI 543
M S ++C I+ + NYP+I++PK +GS+ +TR +KNVG+P TY A + G+ +
Sbjct: 658 MVSEETYKCPKPAISGTNLNYPSITVPKLDGSLVVTRTVKNVGTPGTYKARIQNPDGILV 717
Query: 544 PVQPNILXXXXXXXXXXXXVTIESTRSSVAT--VFGRLTWSDGKHYVRSPIVV 696
V+PN L + ++ + A VFG+L WSDGKHYVRSPIVV
Sbjct: 718 SVEPNKLEFKKIGEEKSFKLLLQVKDAKAAKNYVFGKLIWSDGKHYVRSPIVV 770