BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00016182
         (649 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_013457401.1| cytochrome P450 family 81 protein [Medicago ...   300   5e-97
ref|XP_003605649.2| cytochrome P450 family 81 protein [Medicago ...   291   9e-94
ref|XP_014506448.1| isoflavone 3'-hydroxylase [Vigna radiata var...   281   1e-89
ref|XP_017405622.1| PREDICTED: isoflavone 3'-hydroxylase-like [V...   277   6e-88
ref|XP_003532671.1| PREDICTED: isoflavone 3'-hydroxylase-like [G...   276   8e-88
ref|XP_007158858.1| hypothetical protein PHAVU_002G187800g [Phas...   275   5e-87
ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [C...   254   4e-79
ref|XP_015957960.1| isoflavone 3'-hydroxylase-like [Arachis dura...   253   1e-78
sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase;...   252   3e-78
ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago ...   252   4e-78
ref|XP_020224833.1| LOW QUALITY PROTEIN: isoflavone 3'-hydroxyla...   251   5e-78
ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [C...   251   5e-78
gb|PNX76528.1| isoflavone 2'-hydroxylase-like protein [Trifolium...   246   5e-78
ref|XP_016193629.1| isoflavone 3'-hydroxylase [Arachis ipaensis]      251   8e-78
gb|PNX56086.1| isoflavone 2'-hydroxylase-like protein, partial [...   241   7e-77
dbj|BAT21113.1| cytochrome P450 [synthetic construct]                 248   8e-77
sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase;...   248   1e-76
dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]                244   2e-75
gb|KHN28917.1| Isoflavone 2'-hydroxylase, partial [Glycine soja]      239   3e-75
ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phas...   244   3e-75

>ref|XP_013457401.1| cytochrome P450 family 81 protein [Medicago truncatula]
 gb|KEH31432.1| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 494

 Score =  300 bits (768), Expect = 5e-97
 Identities = 149/216 (68%), Positives = 175/216 (81%), Gaps = 1/216 (0%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAGDVE-GKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXLVKKFK 470
           RLTDMTF  M +MVAGD E GK+FKE+I+EM+PLF ASN               LVK+ K
Sbjct: 177 RLTDMTFEIMTKMVAGDAEEGKKFKEMINEMMPLFGASNMGDFVPLLRLFDFDGLVKRMK 236

Query: 469 SVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTD 290
            +GKR D+FLQ +VEE+R GK   N ML HLLTLQKSQP+YYSD+IIKGL+QGMFL GTD
Sbjct: 237 DIGKRGDSFLQGIVEEIRSGKHGDNNMLQHLLTLQKSQPDYYSDEIIKGLVQGMFLAGTD 296

Query: 289 TSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYS 110
           T+AVT+EWAI+ LLNHP ILKKAKDEL T +G+DR+V+ESDIPNL Y+QNII+ET+RLYS
Sbjct: 297 TTAVTLEWAIATLLNHPNILKKAKDELNTQIGYDRLVEESDIPNLSYIQNIIYETLRLYS 356

Query: 109 PAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           PAPLLLPR+SS E NI GFT+PR+TIVLINAWAI R
Sbjct: 357 PAPLLLPRFSSNECNIEGFTIPRDTIVLINAWAIQR 392


>ref|XP_003605649.2| cytochrome P450 family 81 protein [Medicago truncatula]
 gb|AES87846.2| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 491

 Score =  291 bits (746), Expect = 9e-94
 Identities = 145/216 (67%), Positives = 173/216 (80%), Gaps = 1/216 (0%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAGDV-EGKQFKEIISEMIPLFSASNKAXXXXXXXXXXXXXLVKKFK 470
           RLTDM F+ M  MV GD  E K+FKE++SEM+PLF A+NK              +VKK K
Sbjct: 174 RLTDMMFDNMTAMVVGDAAEFKKFKEMVSEMMPLFGANNKGDFVPLLRLFDFDGMVKKMK 233

Query: 469 SVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQGMFLGGTD 290
            +GKR D FLQ +VEE++ GK + N M+ HLLTLQKSQPEYYSD+IIKGLIQG FLGGTD
Sbjct: 234 DIGKRGDLFLQGIVEEIKSGKHNENTMIQHLLTLQKSQPEYYSDEIIKGLIQGTFLGGTD 293

Query: 289 TSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQNIIHETIRLYS 110
           T   T+EWA+SALLN+PEILKKAKDEL T +G+DR+VDESDI NLPY+QNII+ET+RLYS
Sbjct: 294 TIVTTLEWAMSALLNNPEILKKAKDELYTKIGNDRLVDESDISNLPYIQNIIYETLRLYS 353

Query: 109 PAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           PAPLLLPR+SS+E NI G+T+PR+TIVLINAWAI R
Sbjct: 354 PAPLLLPRFSSDECNIEGYTIPRDTIVLINAWAIQR 389


>ref|XP_014506448.1| isoflavone 3'-hydroxylase [Vigna radiata var. radiata]
          Length = 500

 Score =  281 bits (719), Expect = 1e-89
 Identities = 143/227 (62%), Positives = 174/227 (76%), Gaps = 12/227 (5%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG--------DV----EGKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RLT+MTFN MMRM++G        DV    E KQF+ II+E++ L  A+NK         
Sbjct: 173 RLTEMTFNNMMRMISGKRYYGDDGDVTDAEEAKQFRAIITEVMSLLGANNKGDFLPLLRW 232

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKG 323
                L K+ KS+  RADAFLQ L+EE R GK   N M+ HLLT+Q++QPEYYSD IIKG
Sbjct: 233 FDFDGLEKRLKSISTRADAFLQGLLEEHRSGKHKTNTMIEHLLTMQETQPEYYSDHIIKG 292

Query: 322 LIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQ 143
           LIQGM L GTDT+AVT+EWA+++LLNHPEILKKA+DE++  VG DR+VDESDIPNLPYLQ
Sbjct: 293 LIQGMLLAGTDTTAVTMEWAMASLLNHPEILKKAQDEIDNCVGEDRLVDESDIPNLPYLQ 352

Query: 142 NIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           NII+ET+RL+SPAP+LLP YSSE+F +GGFTVPR+TIVLINAWAI R
Sbjct: 353 NIIYETLRLFSPAPMLLPHYSSEQFTLGGFTVPRDTIVLINAWAIQR 399


>ref|XP_017405622.1| PREDICTED: isoflavone 3'-hydroxylase-like [Vigna angularis]
 gb|KOM25512.1| hypothetical protein LR48_Vigan107s003100 [Vigna angularis]
 dbj|BAT74197.1| hypothetical protein VIGAN_01181600 [Vigna angularis var.
           angularis]
          Length = 500

 Score =  277 bits (708), Expect = 6e-88
 Identities = 141/227 (62%), Positives = 173/227 (76%), Gaps = 12/227 (5%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG--------DV----EGKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RLT+MTFN MMRM++G        DV    E KQF+ II+E++ L  A+NK         
Sbjct: 173 RLTEMTFNNMMRMISGKRYYGDDGDVTDAEEAKQFRAIITEVMSLLGANNKGDFLPLLRW 232

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKG 323
                L K+ KS+  RADAFLQ L+EE R GK   N M+ HLLT+Q++QPEYYSD IIKG
Sbjct: 233 FDFDGLEKRLKSISTRADAFLQGLLEEHRSGKHKTNTMIEHLLTMQETQPEYYSDHIIKG 292

Query: 322 LIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQ 143
           LIQGM L GTDT+AVT+EWA+++LLNHPEILKKA+DE++  VG DR+VDESDIPNLPY+Q
Sbjct: 293 LIQGMLLAGTDTTAVTMEWAMASLLNHPEILKKAQDEIDKCVGQDRLVDESDIPNLPYIQ 352

Query: 142 NIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           NII+ET+RL+SPAP+LLP YSSE+  +GGFTVPR+TIVLINAWAI R
Sbjct: 353 NIIYETLRLFSPAPMLLPHYSSEQCTLGGFTVPRDTIVLINAWAIQR 399


>ref|XP_003532671.1| PREDICTED: isoflavone 3'-hydroxylase-like [Glycine max]
 gb|KHN28918.1| Isoflavone 2'-hydroxylase [Glycine soja]
 gb|KRH42430.1| hypothetical protein GLYMA_08G089400 [Glycine max]
          Length = 500

 Score =  276 bits (707), Expect = 8e-88
 Identities = 140/227 (61%), Positives = 171/227 (75%), Gaps = 12/227 (5%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG------DVEG------KQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RLT+MTFN MMRM++G      D+E       KQF++I++E++ L  A+NK         
Sbjct: 170 RLTEMTFNNMMRMISGKRYYGDDIEAADAEEAKQFRDIMTEVMSLLGANNKGDFLPFLRW 229

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKG 323
                L K+ K +  RAD+FLQ L+EE R GK   N M+ HLLT+Q+SQP YYSD IIKG
Sbjct: 230 FDFDGLEKRLKVISTRADSFLQGLLEEHRSGKHKANTMIEHLLTMQESQPHYYSDHIIKG 289

Query: 322 LIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQ 143
           LIQGM L GTDT+AV IEWA+S+LLNHPEILKKAKDE++ +VG DR+VDESDIP LPYLQ
Sbjct: 290 LIQGMLLAGTDTTAVAIEWAVSSLLNHPEILKKAKDEIDNMVGQDRLVDESDIPKLPYLQ 349

Query: 142 NIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           NII+ET+RL++PAPLLLP YSSEE  IGGFT+PR+TIVLINAWAI R
Sbjct: 350 NIIYETLRLFAPAPLLLPHYSSEECTIGGFTIPRDTIVLINAWAIQR 396


>ref|XP_007158858.1| hypothetical protein PHAVU_002G187800g [Phaseolus vulgaris]
 gb|ESW30852.1| hypothetical protein PHAVU_002G187800g [Phaseolus vulgaris]
          Length = 500

 Score =  275 bits (702), Expect = 5e-87
 Identities = 141/227 (62%), Positives = 171/227 (75%), Gaps = 12/227 (5%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG--------DV----EGKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RLT+MTFN MMRM++G        DV    E KQF++IISE++ L  A+NK         
Sbjct: 173 RLTEMTFNNMMRMISGKRYYGDDGDVTDAEEAKQFRDIISEVMSLLGANNKGDFLPLLRW 232

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKG 323
                L K+ KS+ +RADAFLQ L+EE R GK   N M+ HLLT+Q+SQPEYYSD IIKG
Sbjct: 233 FDFDGLEKRLKSISRRADAFLQGLLEEHRSGKHKANTMIEHLLTMQESQPEYYSDHIIKG 292

Query: 322 LIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQ 143
           LIQGM L GTDT+AVT+EWA+++LLN PEILKKA+DE++  VG DR+V+ESDIPNLPYLQ
Sbjct: 293 LIQGMLLAGTDTTAVTMEWAVASLLNEPEILKKAQDEIKKCVGEDRLVEESDIPNLPYLQ 352

Query: 142 NIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           NII+ET+RL+SPAPLLLP YSS+   IGGFT+P  TIVLINAWAI R
Sbjct: 353 NIIYETLRLFSPAPLLLPHYSSDHCTIGGFTIPPHTIVLINAWAIQR 399


>ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
          Length = 500

 Score =  254 bits (649), Expect = 4e-79
 Identities = 128/228 (56%), Positives = 167/228 (73%), Gaps = 13/228 (5%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG-----------DVE-GKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RLT+MTFN MMRM++G           DVE  KQF++IISE++ L  A+NK         
Sbjct: 176 RLTEMTFNGMMRMISGKRYYGDDGDVTDVEEAKQFRDIISEIMSLLGANNKGDFLPLLRL 235

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDG-KSSGNAMLHHLLTLQKSQPEYYSDQIIK 326
                L K+ K + KRADAFLQ L+EE++ G ++ G+ M+ HLL L++SQPEYYSD +IK
Sbjct: 236 FDLDDLEKRCKRIAKRADAFLQGLIEEIQSGNQNDGDTMIDHLLKLRESQPEYYSDHMIK 295

Query: 325 GLIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYL 146
           GLIQ M L GTDTSAV IEW +S LLNHP++L KAK+E+ET +G D++++E D+  LPYL
Sbjct: 296 GLIQAMLLAGTDTSAVAIEWVMSELLNHPDVLNKAKEEIETQIGRDKLIEEHDLSKLPYL 355

Query: 145 QNIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           QNII+ET+RL+ PAPLLLP YSSE+F +GGF VP++TI+L N WAIHR
Sbjct: 356 QNIINETLRLHPPAPLLLPHYSSEDFTLGGFNVPKDTIILSNVWAIHR 403


>ref|XP_015957960.1| isoflavone 3'-hydroxylase-like [Arachis duranensis]
          Length = 508

 Score =  253 bits (646), Expect = 1e-78
 Identities = 128/230 (55%), Positives = 166/230 (72%), Gaps = 15/230 (6%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG--------DVEGK------QFKEIISEMIPLFSASNKAXXXXXX 509
           R+T+M+ N MMRMV+G        D  G+      +F+EII E++ L  A+NK+      
Sbjct: 178 RITEMSLNNMMRMVSGKRNEYGKDDCNGRDDNRAGRFREIIREILSLVDANNKSDFLPLL 237

Query: 508 XXXXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGN-AMLHHLLTLQKSQPEYYSDQI 332
                   VK+ K VG+ AD F Q L+EE R+G+   N  M+ HLL LQK QP YY+D I
Sbjct: 238 RWFDFDGFVKRLKRVGEEADVFWQGLLEEHRNGEHGNNDTMIQHLLLLQKLQPNYYTDHI 297

Query: 331 IKGLIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLP 152
           IKGL+Q M L GT+T+ +T+EW +SALLNHPEIL+KAK+E++  +G DR+VDESDIPNLP
Sbjct: 298 IKGLVQDMLLAGTNTTVLTMEWTLSALLNHPEILRKAKEEIDNHIGKDRLVDESDIPNLP 357

Query: 151 YLQNIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           YLQNIIHET+RLYSP+PLLLP +SS+E NIGGFT+PR+TIVLIN+WA+ R
Sbjct: 358 YLQNIIHETLRLYSPSPLLLPHFSSDEVNIGGFTIPRDTIVLINSWAMRR 407


>sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase; AltName: Full=Cytochrome
           P450 81E9
 gb|AAQ20041.1| isoflavone 3'-hydroxylase, partial [Medicago truncatula]
          Length = 501

 Score =  252 bits (643), Expect = 3e-78
 Identities = 132/229 (57%), Positives = 162/229 (70%), Gaps = 14/229 (6%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG-----------DVE-GKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RLT+MTFN MMRM++G           DVE  KQF+EIISEM+ L  A+NK         
Sbjct: 176 RLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRV 235

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGK--SSGNAMLHHLLTLQKSQPEYYSDQII 329
                L K+ K + KR++AFL+ L+EE R G   S G  M+ HLL L +SQPEYYSD +I
Sbjct: 236 VDLDNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLI 295

Query: 328 KGLIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPY 149
           KGLIQGM L GTDTSAVTIEW +S LLNHPE+LKKAK+EL+T +G +++VDE D+  LPY
Sbjct: 296 KGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPY 355

Query: 148 LQNIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           LQNII ET+RL+ PAPLLLP YSSE+  IG F VP++TI+L N W IHR
Sbjct: 356 LQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHR 404


>ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago truncatula]
 gb|KEH37344.1| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 503

 Score =  252 bits (643), Expect = 4e-78
 Identities = 132/229 (57%), Positives = 162/229 (70%), Gaps = 14/229 (6%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG-----------DVE-GKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RLT+MTFN MMRM++G           DVE  KQF+EIISEM+ L  A+NK         
Sbjct: 178 RLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRL 237

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGK--SSGNAMLHHLLTLQKSQPEYYSDQII 329
                L K+ K + KR++AFL+ L+EE R G   S G  M+ HLL L +SQPEYYSD +I
Sbjct: 238 FDLDNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLI 297

Query: 328 KGLIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPY 149
           KGLIQGM L GTDTSAVTIEW +S LLNHPE+LKKAK+EL+T +G +++VDE D+  LPY
Sbjct: 298 KGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPY 357

Query: 148 LQNIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           LQNII ET+RL+ PAPLLLP YSSE+  IG F VP++TI+L N W IHR
Sbjct: 358 LQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHR 406


>ref|XP_020224833.1| LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cajanus cajan]
          Length = 500

 Score =  251 bits (642), Expect = 5e-78
 Identities = 136/228 (59%), Positives = 162/228 (71%), Gaps = 14/228 (6%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG--------DV----EGKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RLT+MTFN MMRM++G        DV    E KQF+EIISEM+ L  A+NK         
Sbjct: 176 RLTEMTFNAMMRMISGKRYYGDDCDVTDAEEAKQFREIISEMLSLLGANNKGDFLPLLRL 235

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGNA--MLHHLLTLQKSQPEYYSDQII 329
                L K+ K + KRADAFLQ L+EE R GK  GNA  M+ HLL L +SQPEYYSD +I
Sbjct: 236 FDFDDLEKRLKRIAKRADAFLQSLIEEHRSGKH-GNADTMIDHLLKLGESQPEYYSDHMI 294

Query: 328 KGLIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPY 149
           KGLIQ M L GTDTSAVTIEW +S LLN+PE+LKKAKDE++T +G DR+V+E D+  LPY
Sbjct: 295 KGLIQAMLLAGTDTSAVTIEWVMSELLNNPEVLKKAKDEIDTQIGKDRLVEEQDLSKLPY 354

Query: 148 LQNIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIH 5
           LQNII ET+RL+ PAPLLLP  SSE+  IGG+ +PR TIVL NAW IH
Sbjct: 355 LQNIISETLRLHPPAPLLLPHESSEDCTIGGYHIPRNTIVLTNAWLIH 402


>ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
          Length = 500

 Score =  251 bits (642), Expect = 5e-78
 Identities = 129/228 (56%), Positives = 167/228 (73%), Gaps = 13/228 (5%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG-----------DVE-GKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RLT+MTFN MM M++G           DVE  KQF++IISE++ L  A NK+        
Sbjct: 177 RLTEMTFNGMMMMISGKRYYGDDRDVTDVEEAKQFRDIISEIMSLLGA-NKSEFLPFLRL 235

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDG-KSSGNAMLHHLLTLQKSQPEYYSDQIIK 326
                L K++K + KRADAFLQ L+EE + G +S+G+ M+ HLL L+KSQPEYYSD +IK
Sbjct: 236 FDLDDLTKRYKKIAKRADAFLQGLIEEHQSGNQSNGDTMIDHLLNLRKSQPEYYSDHMIK 295

Query: 325 GLIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYL 146
           GLIQ M L GTDTSAVTIEW +S LLNHP++L KAK+E+ET +G D++++E D+  LPYL
Sbjct: 296 GLIQAMLLAGTDTSAVTIEWVMSELLNHPDVLNKAKEEIETQIGKDKLIEEHDLSKLPYL 355

Query: 145 QNIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           QNII+ET+RL+ PAPL LP YSSE+F +GGF VP++TI+L N WAIHR
Sbjct: 356 QNIINETLRLHPPAPLSLPHYSSEDFTLGGFNVPKDTIILTNVWAIHR 403


>gb|PNX76528.1| isoflavone 2'-hydroxylase-like protein [Trifolium pratense]
          Length = 328

 Score =  246 bits (628), Expect = 5e-78
 Identities = 121/227 (53%), Positives = 161/227 (70%), Gaps = 12/227 (5%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG-----------DVEG-KQFKEIISEMIPLFSASNKAXXXXXXXX 503
           + ++MTFNT+MRM++G           D EG K+F+E+I EM+ L  +SN          
Sbjct: 4   KFSEMTFNTIMRMISGKRYYGEDCDVSDEEGAKEFRELIKEMVSLGGSSNPGEFVGIFRL 63

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKG 323
                  KK K + +R D FLQ L++E+R  K +GN M+ HLL LQ+SQPEYY+DQIIKG
Sbjct: 64  FDFGDYEKKLKRISRRFDGFLQGLIDEIRRKKENGNTMIDHLLNLQESQPEYYTDQIIKG 123

Query: 322 LIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQ 143
           ++  M LGGT+TSA T+EWA+SALLNHPE+LKKA+DE++  +GHDR+V+ESDI NLPY+Q
Sbjct: 124 IVLVMILGGTETSATTLEWAMSALLNHPEVLKKARDEIDANIGHDRLVEESDISNLPYIQ 183

Query: 142 NIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           NIIHET RL+    LL P +SS +  IGG+ VP+ TI+L+NAWAIHR
Sbjct: 184 NIIHETFRLHPAFALLAPHFSSTDCTIGGYNVPKGTILLVNAWAIHR 230


>ref|XP_016193629.1| isoflavone 3'-hydroxylase [Arachis ipaensis]
          Length = 508

 Score =  251 bits (641), Expect = 8e-78
 Identities = 128/230 (55%), Positives = 163/230 (70%), Gaps = 15/230 (6%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAG--------------DVEGKQFKEIISEMIPLFSASNKAXXXXXX 509
           R+T+M+ N MMRMV+G              D    +F+EII E++ L  A+NK+      
Sbjct: 178 RITEMSLNNMMRMVSGKRNEYRKDDCNARDDNGAGRFREIIREILSLVDANNKSDFLPLL 237

Query: 508 XXXXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGN-AMLHHLLTLQKSQPEYYSDQI 332
                   VK+ K VG+ AD F Q L+EE R+G+   N  M+ HLL LQK QP YY+D I
Sbjct: 238 RWFDFDGFVKRLKRVGEEADMFWQGLLEEHRNGEHGNNDTMIQHLLLLQKLQPNYYTDHI 297

Query: 331 IKGLIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLP 152
           IKGLIQ M L GT+T+ +T+EW +SALLNHPEIL+KAKDE++  +G DR+VDESDIP LP
Sbjct: 298 IKGLIQDMLLAGTNTTVLTMEWTLSALLNHPEILRKAKDEIDNHIGKDRLVDESDIPKLP 357

Query: 151 YLQNIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           YLQNIIHET+RLYSP+PLLLP +SS+E NIGGFT+PR+TIVLIN+WA+ R
Sbjct: 358 YLQNIIHETLRLYSPSPLLLPHFSSDEVNIGGFTIPRDTIVLINSWAMRR 407


>gb|PNX56086.1| isoflavone 2'-hydroxylase-like protein, partial [Trifolium
           pratense]
          Length = 258

 Score =  241 bits (614), Expect = 7e-77
 Identities = 126/225 (56%), Positives = 158/225 (70%), Gaps = 14/225 (6%)
 Frame = -1

Query: 634 MTFNTMMRMVAG-----------DVE-GKQFKEIISEMIPLFSASNKAXXXXXXXXXXXX 491
           MTFN+MMRM++G           DVE  KQF+EIISE++ L  A+NK             
Sbjct: 1   MTFNSMMRMISGKRYYGDDGDVTDVEEAKQFREIISEIMSLLGANNKGDFLPLLRFLDLD 60

Query: 490 XLVKKFKSVGKRADAFLQELVEELRDGK--SSGNAMLHHLLTLQKSQPEYYSDQIIKGLI 317
            L K+ K + KRADAFL+ L+EE R G   S GN M+ +LL L + QPEYYS+ IIKGLI
Sbjct: 61  NLEKRCKRIAKRADAFLEGLIEEHRSGNHNSDGNTMIDNLLKLGEIQPEYYSNHIIKGLI 120

Query: 316 QGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQNI 137
           Q M L GTDTSAVTIEW +S LLNHPE+L KAK+EL+T +G ++++DE D+  LPYLQNI
Sbjct: 121 QAMLLAGTDTSAVTIEWIMSELLNHPEVLNKAKEELDTQIGKNKLIDEHDLSKLPYLQNI 180

Query: 136 IHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
             ET+RL+ PAPLLLP YSSE+F +G F VP++TI+L N WAIHR
Sbjct: 181 FSETLRLHPPAPLLLPHYSSEDFTLGEFNVPKDTIILTNVWAIHR 225


>dbj|BAT21113.1| cytochrome P450 [synthetic construct]
          Length = 477

 Score =  248 bits (632), Expect = 8e-77
 Identities = 125/227 (55%), Positives = 160/227 (70%), Gaps = 12/227 (5%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAGD------------VEGKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RL DMTFN +MRM++G              E  QF++++SE++ L  A+NK         
Sbjct: 153 RLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRF 212

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKG 323
                L K+ K +  + DAFL+ L+EE R  K   N M+ HLL LQ SQPEYY+DQIIKG
Sbjct: 213 LDFENLEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKG 272

Query: 322 LIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQ 143
           L   M L GTD+SAVT+EW++S LLNHPE+LKK KDEL+T VG DR+VDESD+P L YL+
Sbjct: 273 LALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLK 332

Query: 142 NIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           N+I+ET+RLY+PAPLLLP  +S+E NIGG+ VP++TIVLINAWAIHR
Sbjct: 333 NVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHR 379


>sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase; AltName: Full=CYP GE-3;
           AltName: Full=Cytochrome P450 81E1; AltName:
           Full=Cytochrome P450 91A4
 dbj|BAA22422.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  248 bits (632), Expect = 1e-76
 Identities = 125/227 (55%), Positives = 160/227 (70%), Gaps = 12/227 (5%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAGD------------VEGKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           RL DMTFN +MRM++G              E  QF++++SE++ L  A+NK         
Sbjct: 175 RLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRF 234

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKG 323
                L K+ K +  + DAFL+ L+EE R  K   N M+ HLL LQ SQPEYY+DQIIKG
Sbjct: 235 LDFENLEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKG 294

Query: 322 LIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQ 143
           L   M L GTD+SAVT+EW++S LLNHPE+LKK KDEL+T VG DR+VDESD+P L YL+
Sbjct: 295 LALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLK 354

Query: 142 NIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           N+I+ET+RLY+PAPLLLP  +S+E NIGG+ VP++TIVLINAWAIHR
Sbjct: 355 NVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHR 401


>dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  244 bits (624), Expect = 2e-75
 Identities = 123/227 (54%), Positives = 159/227 (70%), Gaps = 12/227 (5%)
 Frame = -1

Query: 646 RLTDMTFNTMMRMVAGD------------VEGKQFKEIISEMIPLFSASNKAXXXXXXXX 503
           R  DMTFN +MRM++G              E  QF++++SE++ L  A+NK         
Sbjct: 175 RFYDMTFNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRF 234

Query: 502 XXXXXLVKKFKSVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKG 323
                L K+ K +  + DAFL+ L++E R  K   N M+ HLL LQ SQPEYY+DQIIKG
Sbjct: 235 LDFENLEKRLKDISGKTDAFLRGLIQEHRAKKERANTMIDHLLNLQDSQPEYYTDQIIKG 294

Query: 322 LIQGMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQ 143
           L   M L GTD+SAVT+EW++S LLNHPE+LKK KDEL+T VG DR+VDESD+P L YL+
Sbjct: 295 LALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLSYLK 354

Query: 142 NIIHETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           N+I+ET+RLY+PAPLLLP  +S+E NIGG+ VP++TIVLINAWAIHR
Sbjct: 355 NVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHR 401


>gb|KHN28917.1| Isoflavone 2'-hydroxylase, partial [Glycine soja]
          Length = 329

 Score =  239 bits (610), Expect = 3e-75
 Identities = 122/224 (54%), Positives = 158/224 (70%), Gaps = 12/224 (5%)
 Frame = -1

Query: 637 DMTFNTMMRMVAG-----------DVE-GKQFKEIISEMIPLFSASNKAXXXXXXXXXXX 494
           DMTFN +MRM++G           DVE  KQF+ ++SE++ L  A+NK            
Sbjct: 9   DMTFNNIMRMISGKRYYGDDCDMADVEEAKQFRAMVSELLKLAGANNKNDFMPVLRLFDF 68

Query: 493 XXLVKKFKSVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQ 314
             L K+ K +  + D FL+ L+EE+R  K   N ML HLL+LQ+SQPEYY+DQIIKGL  
Sbjct: 69  ENLEKRLKKISNKTDTFLRGLLEEIRAKKQRANTMLDHLLSLQESQPEYYTDQIIKGLAL 128

Query: 313 GMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQNII 134
           GM +  TD+ AVT+EWA+S +LNHPE+ K+A+DELET VG D +++ESD+  LPYL+NII
Sbjct: 129 GMLIAATDSQAVTLEWALSCVLNHPEVFKRARDELETHVGQDHLLEESDLSKLPYLKNII 188

Query: 133 HETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
           +ET+RLY+PAPLLLP  SSEE  IGGF VP +TIVLINAW+IHR
Sbjct: 189 YETLRLYTPAPLLLPHSSSEECIIGGFKVPGDTIVLINAWSIHR 232


>ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
 gb|ESW30851.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
          Length = 500

 Score =  244 bits (623), Expect = 3e-75
 Identities = 123/224 (54%), Positives = 161/224 (71%), Gaps = 12/224 (5%)
 Frame = -1

Query: 637 DMTFNTMMRMVAG-----------DVE-GKQFKEIISEMIPLFSASNKAXXXXXXXXXXX 494
           DMTFN +MRM++G           DVE  +QF+ ++SE++ L  A+NK            
Sbjct: 180 DMTFNNIMRMISGKRYYGDDCDMADVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDF 239

Query: 493 XXLVKKFKSVGKRADAFLQELVEELRDGKSSGNAMLHHLLTLQKSQPEYYSDQIIKGLIQ 314
             L K+ K +  + D FL  L+EE R  K   N M+ HLL+LQ+SQPEYY+DQIIKGL  
Sbjct: 240 ENLEKRLKKISSKTDTFLNGLLEEHRSKKQRANTMVDHLLSLQESQPEYYTDQIIKGLAL 299

Query: 313 GMFLGGTDTSAVTIEWAISALLNHPEILKKAKDELETVVGHDRMVDESDIPNLPYLQNII 134
           GM + GTD+SAVT+EWA+S ++NHPE++K+A+DELET VG +R++DESD+  LPYL+NII
Sbjct: 300 GMLIAGTDSSAVTLEWALSCVVNHPEVMKRARDELETHVGEERLLDESDLSKLPYLKNII 359

Query: 133 HETIRLYSPAPLLLPRYSSEEFNIGGFTVPRETIVLINAWAIHR 2
            ET+RLY+PAPLLLP  SSEE NIGGF VPR++IVLINAW+IHR
Sbjct: 360 FETLRLYTPAPLLLPHSSSEECNIGGFKVPRDSIVLINAWSIHR 403