BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00015977
         (319 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004486105.1| PREDICTED: F-box/kelch-repeat protein At3g23...    99   2e-22
ref|XP_004486107.1| PREDICTED: F-box/kelch-repeat protein At3g23...    98   6e-22
gb|PNX73964.1| F-box/kelch-repeat protein [Trifolium pratense]         95   6e-21
ref|XP_003594508.2| F-box protein interaction domain protein [Me...    95   8e-21
ref|XP_013447824.1| F-box protein interaction domain protein [Me...    94   3e-20
dbj|GAU24894.1| hypothetical protein TSUD_116180 [Trifolium subt...    92   8e-20
ref|XP_013459319.1| F-box protein interaction domain protein [Me...    92   1e-19
ref|XP_003621455.1| F-box protein interaction domain protein [Me...    92   1e-19
gb|PNX81830.1| F-box/kelch-repeat protein, partial [Trifolium pr...    89   2e-19
ref|XP_013459075.1| F-box protein interaction domain protein [Me...    89   7e-19
gb|PNX83638.1| F-box/kelch-repeat protein [Trifolium pratense]         89   9e-19
gb|KRH11341.1| hypothetical protein GLYMA_15G101700 [Glycine max]      89   1e-18
ref|NP_001304395.1| F-box/kelch-repeat protein At3g23880-like [G...    89   1e-18
dbj|GAU20086.1| hypothetical protein TSUD_381780 [Trifolium subt...    89   1e-18
dbj|GAU24895.1| hypothetical protein TSUD_116190 [Trifolium subt...    88   2e-18
ref|XP_019439762.1| PREDICTED: F-box/kelch-repeat protein At3g23...    87   5e-18
dbj|GAU20920.1| hypothetical protein TSUD_24830 [Trifolium subte...    87   5e-18
ref|XP_003594087.1| F-box protein interaction domain protein [Me...    87   6e-18
ref|XP_003598388.1| F-box and associated interaction domain prot...    87   7e-18
ref|XP_019420066.1| PREDICTED: F-box/kelch-repeat protein At3g23...    87   8e-18

>ref|XP_004486105.1| PREDICTED: F-box/kelch-repeat protein At3g23880-like [Cicer
           arietinum]
          Length = 384

 Score = 99.4 bits (246), Expect = 2e-22
 Identities = 57/110 (51%), Positives = 69/110 (62%), Gaps = 4/110 (3%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRVGGSFN----WVCKKVEVHALGTNTWKTIQGLPPAFP 168
           TIY FGYD  SD YKVV+ SCYR   SFN    +   KV++H LGTN+W+ IQ  P   P
Sbjct: 167 TIYAFGYDHFSDTYKVVSVSCYR---SFNLTDCFFKTKVKIHTLGTNSWRRIQDFPTGVP 223

Query: 169 FGLTVRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
           F     E G F++GTINWL  T S   + VI S +L++E YQELL PD G
Sbjct: 224 FEFD--ESGKFVNGTINWLASTNSS-SLWVIVSLNLKDECYQELLLPDYG 270


>ref|XP_004486107.1| PREDICTED: F-box/kelch-repeat protein At3g23880-like [Cicer
           arietinum]
          Length = 405

 Score = 98.2 bits (243), Expect = 6e-22
 Identities = 58/110 (52%), Positives = 67/110 (60%), Gaps = 4/110 (3%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRV---GGSFNWVCK-KVEVHALGTNTWKTIQGLPPAFP 168
           TIY FGY   SD+YKVVA SCY     G + N V K +V+VH LGTN+W+ IQ  P   P
Sbjct: 186 TIYAFGYAHFSDSYKVVAVSCYESDTNGSTSNRVYKTEVKVHTLGTNSWRRIQDFPSGVP 245

Query: 169 FGLTVRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
           F     E G F++GT+NWL  T   I   VI S DL  ESYQELL PD G
Sbjct: 246 FD----ESGKFVNGTVNWLAST-DWISSWVIVSLDLEKESYQELLQPDYG 290


>gb|PNX73964.1| F-box/kelch-repeat protein [Trifolium pratense]
          Length = 359

 Score = 95.1 bits (235), Expect = 6e-21
 Identities = 54/111 (48%), Positives = 69/111 (62%), Gaps = 5/111 (4%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRVGGSFNWVCKKVEVHALGTNTWKTIQGLPPAFPFGLT 180
           T+YGFGYDR +DNYKVVA SCY  G +      KV VH +GTN W+ IQ     FP G+ 
Sbjct: 144 TMYGFGYDRYNDNYKVVAISCYDSGKT------KVNVHTMGTNCWRRIQ----EFPSGVV 193

Query: 181 -VRERGIFLSGTINWL----GITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
            V E G F+ GT+NWL    G+  +   + ++ S DL NESYQ++L PD G
Sbjct: 194 PVGESGKFVGGTVNWLASENGVELNSSSLCIV-SLDLGNESYQKILQPDYG 243


>ref|XP_003594508.2| F-box protein interaction domain protein [Medicago truncatula]
 gb|AES64759.2| F-box protein interaction domain protein [Medicago truncatula]
          Length = 405

 Score = 95.1 bits (235), Expect = 8e-21
 Identities = 52/107 (48%), Positives = 64/107 (59%), Gaps = 1/107 (0%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRV-GGSFNWVCKKVEVHALGTNTWKTIQGLPPAFPFGL 177
           TIYGFGYD ++D YKVVA  C+    G +     +V+VH LGTN W+ I  LP    FG+
Sbjct: 191 TIYGFGYDHVNDIYKVVAVYCFESDNGDYK---TQVKVHTLGTNFWRRIHDLP----FGV 243

Query: 178 TVRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
              E G F+SGT+NWL    S     +I S DL  E+YQELL PD G
Sbjct: 244 PFDESGKFVSGTVNWLASNDSSYTSSIIVSLDLEKETYQELLQPDYG 290


>ref|XP_013447824.1| F-box protein interaction domain protein [Medicago truncatula]
 gb|KEH21502.1| F-box protein interaction domain protein [Medicago truncatula]
          Length = 401

 Score = 93.6 bits (231), Expect = 3e-20
 Identities = 57/112 (50%), Positives = 72/112 (64%), Gaps = 6/112 (5%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCY--RVGGSF-NWVCK-KVEVHALGTNTWKTIQGLPPAFP 168
           T  GFGYD  ++NYKVV    Y  RVG  + ++V K +V++H LGTN+WK+IQ     FP
Sbjct: 181 TTLGFGYDSFTENYKVVVVMRYKMRVGSHYRDYVYKTEVKIHTLGTNSWKSIQ----EFP 236

Query: 169 FGLT-VRERGIFLSGTINWL-GITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
           FG+  + + G FLSGTINWL  I       R I SFDL  ESYQ++LPPD G
Sbjct: 237 FGVVPIGQSGKFLSGTINWLTSIDLQRESPRFIVSFDLGKESYQKVLPPDYG 288


>dbj|GAU24894.1| hypothetical protein TSUD_116180 [Trifolium subterraneum]
          Length = 360

 Score = 92.0 bits (227), Expect = 8e-20
 Identities = 53/110 (48%), Positives = 67/110 (60%), Gaps = 5/110 (4%)
 Frame = +1

Query: 4   IYGFGYDRLSDNYKVVAFSCYRVGGSFNWVCKKVEVHALGTNTWKTIQGLPPAFPFGLT- 180
           IYGFGYDR +DNYKVVA  CY  G S      KV VH +GTN W+ IQ     FP G+  
Sbjct: 146 IYGFGYDRYNDNYKVVAIFCYEDGKS------KVNVHTMGTNCWRKIQ----EFPCGVVP 195

Query: 181 VRERGIFLSGTINWL----GITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
           V E G F++GT+NWL    G+  +   + ++ S DL NESYQ++  PD G
Sbjct: 196 VGESGKFINGTVNWLASENGVELNSSSLCIV-SLDLGNESYQKISQPDYG 244


>ref|XP_013459319.1| F-box protein interaction domain protein [Medicago truncatula]
 gb|KEH33350.1| F-box protein interaction domain protein [Medicago truncatula]
          Length = 395

 Score = 92.0 bits (227), Expect = 1e-19
 Identities = 53/109 (48%), Positives = 69/109 (63%), Gaps = 3/109 (2%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRVG-GSFNWVCK-KVEVHALGTNTWKTIQGLPPAFPFG 174
           T  GFGYD  +DNYKVV    Y +  GS ++V K +V++H LGT+ WK IQ     FPFG
Sbjct: 180 TTLGFGYDSCTDNYKVVVLMRYEMRVGSGDYVYKTEVKIHILGTDFWKNIQ----EFPFG 235

Query: 175 LTVRERGIFLSGTINWL-GITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
           +    +  F+SGTINWL  I   P   R I SFDL  ES++++LPPD+G
Sbjct: 236 VVPIGQSKFVSGTINWLTSIDLDPKSPRFIVSFDLGKESFRKVLPPDNG 284


>ref|XP_003621455.1| F-box protein interaction domain protein [Medicago truncatula]
 gb|AES77673.1| F-box protein interaction domain protein [Medicago truncatula]
          Length = 404

 Score = 92.0 bits (227), Expect = 1e-19
 Identities = 52/107 (48%), Positives = 61/107 (57%), Gaps = 1/107 (0%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYR-VGGSFNWVCKKVEVHALGTNTWKTIQGLPPAFPFGL 177
           T+Y  GYDR  DNYKVVAFSC+R +  S+ +   +V VH LGTN W+ I   P     GL
Sbjct: 172 TLYSIGYDRFVDNYKVVAFSCHRQINKSYKYCNSQVRVHTLGTNFWRRIPNFPSNI-MGL 230

Query: 178 TVRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
                G F+SGTINW           VI S DL NESYQE+  PD G
Sbjct: 231 PNGYVGKFVSGTINWAIENQKNYDSWVILSLDLGNESYQEISRPDFG 277


>gb|PNX81830.1| F-box/kelch-repeat protein, partial [Trifolium pratense]
          Length = 248

 Score = 89.4 bits (220), Expect = 2e-19
 Identities = 53/105 (50%), Positives = 67/105 (63%), Gaps = 1/105 (0%)
 Frame = +1

Query: 4   IYGFGYDRLSDNYKVVAFSCYRVGGSFNWVCKKVEVHALGTNTWKTIQGLPPAFPFGLT- 180
           IYGFGYD +SDNYKVV  S     G FN    +++VH LGT++WK++      FPF +  
Sbjct: 150 IYGFGYDPISDNYKVVVVSSDLTRGDFN--KNELKVHTLGTDSWKSVS----EFPFAIVY 203

Query: 181 VRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPPDD 315
            +  G ++SGTINWL   F  IK R I SFDL NE Y+E+L PDD
Sbjct: 204 AQPLGQYVSGTINWL--VFMGIK-RFIASFDLGNECYREVLLPDD 245


>ref|XP_013459075.1| F-box protein interaction domain protein [Medicago truncatula]
 gb|KEH33128.1| F-box protein interaction domain protein [Medicago truncatula]
          Length = 319

 Score = 89.0 bits (219), Expect = 7e-19
 Identities = 56/106 (52%), Positives = 64/106 (60%), Gaps = 4/106 (3%)
 Frame = +1

Query: 7   YGFGYDRLSDNYKV-VAFSCYRVGGSFNWVCK-KVEVHALGTNTWKTIQGLPPAFPF-GL 177
           +GFGYD  SDNYKV + F C     S N V K KV+VH LGTN WK I    P FPF G+
Sbjct: 105 FGFGYDSFSDNYKVIIVFRCSIRESSGNLVNKTKVKVHTLGTNYWKII----PEFPFGGV 160

Query: 178 TVRERGIFLSGTINWL-GITFSPIKMRVIFSFDLRNESYQELLPPD 312
            +   G F+SGTINWL  I F       I S+DL  ESYQE+L PD
Sbjct: 161 PIERTGKFVSGTINWLTSIEFYRESPYFIVSYDLGKESYQEVLLPD 206


>gb|PNX83638.1| F-box/kelch-repeat protein [Trifolium pratense]
          Length = 380

 Score = 89.4 bits (220), Expect = 9e-19
 Identities = 52/109 (47%), Positives = 68/109 (62%), Gaps = 5/109 (4%)
 Frame = +1

Query: 7   YGFGYDRLSDNYKVVAFSCYRVGGSFNWVCKK--VEVHALGTNTWKTIQGLPPAFPF-GL 177
           +GFGYD  +DNYKVV    Y +   F    +K  V+VH LGTN WK IQ     FPF G+
Sbjct: 165 FGFGYDSFTDNYKVVVVFEYHIYDRFGMRIRKSQVKVHTLGTNFWKNIQD----FPFDGV 220

Query: 178 TVRERGIFLSGTINWLGITFSPIKMRVIF--SFDLRNESYQELLPPDDG 318
            + + G F+SGT+NWL  +F   ++R  F  S DL+NESYQ++L PD G
Sbjct: 221 LIEKSGKFVSGTLNWLA-SFKLYRLRPCFIVSLDLKNESYQKVLLPDFG 268


>gb|KRH11341.1| hypothetical protein GLYMA_15G101700 [Glycine max]
          Length = 393

 Score = 89.4 bits (220), Expect = 1e-18
 Identities = 49/103 (47%), Positives = 63/103 (61%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRVGGSFNWVCKKVEVHALGTNTWKTIQGLPPAFPFGLT 180
           TI+GFGYDR +D+YKVVA  CY   G +     +V+V  LGT++W+ IQ     FP GL 
Sbjct: 185 TIHGFGYDRFADSYKVVAIFCYECDGRYE---TQVKVLTLGTDSWRRIQ----EFPSGLP 237

Query: 181 VRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPP 309
             E G F+SGT+NWL    S     +I S DL  ESY+E+L P
Sbjct: 238 FDESGKFVSGTVNWLASNDS--SSLIIVSLDLHKESYEEVLQP 278


>ref|NP_001304395.1| F-box/kelch-repeat protein At3g23880-like [Glycine max]
 gb|ACU22680.1| unknown [Glycine max]
          Length = 393

 Score = 89.4 bits (220), Expect = 1e-18
 Identities = 49/103 (47%), Positives = 63/103 (61%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRVGGSFNWVCKKVEVHALGTNTWKTIQGLPPAFPFGLT 180
           TI+GFGYDR +D+YKVVA  CY   G +     +V+V  LGT++W+ IQ     FP GL 
Sbjct: 185 TIHGFGYDRFADSYKVVAIFCYECDGRYE---TQVKVLTLGTDSWRRIQ----EFPSGLP 237

Query: 181 VRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPP 309
             E G F+SGT+NWL    S     +I S DL  ESY+E+L P
Sbjct: 238 FDESGKFVSGTVNWLASNDS--SSLIIVSLDLHKESYEEVLQP 278


>dbj|GAU20086.1| hypothetical protein TSUD_381780 [Trifolium subterraneum]
          Length = 406

 Score = 89.4 bits (220), Expect = 1e-18
 Identities = 53/110 (48%), Positives = 64/110 (58%), Gaps = 4/110 (3%)
 Frame = +1

Query: 1   TIYGFGY--DRLSDNY--KVVAFSCYRVGGSFNWVCKKVEVHALGTNTWKTIQGLPPAFP 168
           TIYGFGY  +  +DNY  KVVA +CY            V+VH LGTN+W+ I+  P   P
Sbjct: 189 TIYGFGYCYNNFNDNYSYKVVAVNCYESESDNRVYKTNVKVHTLGTNSWRWIEDFPSGVP 248

Query: 169 FGLTVRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
           F  +    G F+SGTINWL    S   + VI S DL NESYQEL+ PD G
Sbjct: 249 FDFS----GRFVSGTINWLAAKDSYTSL-VIVSLDLENESYQELVQPDYG 293


>dbj|GAU24895.1| hypothetical protein TSUD_116190 [Trifolium subterraneum]
          Length = 367

 Score = 88.2 bits (217), Expect = 2e-18
 Identities = 54/113 (47%), Positives = 66/113 (58%), Gaps = 7/113 (6%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRVGGSFNWVCKKVEVHALGTNTWKTIQGLPPAFPFG-L 177
           T+YGFGYDR +D+YKVVA  CY  G +      KV VH +GTN W+ IQ     FPFG +
Sbjct: 146 TMYGFGYDRCNDSYKVVAIFCYDNGKT------KVNVHTMGTNCWRKIQ----EFPFGSV 195

Query: 178 TVRERGIFLSGTINWL------GITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
            V E G F++GT+NWL      GI    I    I S DL NE Y+ +L PD G
Sbjct: 196 PVGESGKFVNGTVNWLASGNENGIRL--ISSSSIVSLDLGNECYRMILQPDYG 246


>ref|XP_019439762.1| PREDICTED: F-box/kelch-repeat protein At3g23880-like [Lupinus
           angustifolius]
          Length = 386

 Score = 87.4 bits (215), Expect = 5e-18
 Identities = 50/106 (47%), Positives = 62/106 (58%), Gaps = 2/106 (1%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSC--YRVGGSFNWVCKKVEVHALGTNTWKTIQGLPPAFPFG 174
           TIYGFG+D+ SD+YKVVA     Y       WV + V+VH LGT++W+TIQG P   P G
Sbjct: 167 TIYGFGFDQCSDSYKVVAILRHEYYSDDESAWVTE-VKVHNLGTDSWRTIQGCPSGIPTG 225

Query: 175 LTVRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPPD 312
            +      F+SGT+NWL      +    I S DL  ES QELL PD
Sbjct: 226 FSAT----FVSGTLNWLASNDPDLSSYAIVSLDLGKESCQELLQPD 267


>dbj|GAU20920.1| hypothetical protein TSUD_24830 [Trifolium subterraneum]
          Length = 386

 Score = 87.4 bits (215), Expect = 5e-18
 Identities = 47/111 (42%), Positives = 64/111 (57%), Gaps = 5/111 (4%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRVGGSFNWVCK-KVEVHALGTNTWKTIQGLPPAFPFGL 177
           T+YGFGYD   DNYKVV+   Y V     + CK +V V++LGT+ W+ I+  PP  P+G 
Sbjct: 169 TVYGFGYDPFVDNYKVVSVFYYNV-----YACKTEVSVYSLGTDCWRRIEDFPPLIPYG- 222

Query: 178 TVRERGIFLSGTINWLGI----TFSPIKMRVIFSFDLRNESYQELLPPDDG 318
               +G+F+ GT+NWL           +   I S DL NE+Y E+L PD G
Sbjct: 223 ---RQGVFVCGTVNWLAYYDLNENDYYRSYAIVSLDLENETYHEILQPDYG 270


>ref|XP_003594087.1| F-box protein interaction domain protein [Medicago truncatula]
 gb|AES64338.1| F-box protein interaction domain protein [Medicago truncatula]
          Length = 375

 Score = 87.0 bits (214), Expect = 6e-18
 Identities = 51/109 (46%), Positives = 62/109 (56%), Gaps = 3/109 (2%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRVGGSFNW--VCK-KVEVHALGTNTWKTIQGLPPAFPF 171
           TIYGFGYD  +D YKVVA +C+      N   V K +V+V+ LGT+ W+ IQ  P   PF
Sbjct: 159 TIYGFGYDHFNDTYKVVAVNCFESDTDSNGSKVYKTEVKVYTLGTDYWRRIQDFPSGVPF 218

Query: 172 GLTVRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
                  G F+SGTINWL     P    +I S DL  E+YQ LL PD G
Sbjct: 219 D----NSGTFVSGTINWLAAK-DPYTSWIIVSLDLEEETYQYLLQPDYG 262


>ref|XP_003598388.1| F-box and associated interaction domain protein [Medicago
           truncatula]
 gb|AES68639.1| F-box and associated interaction domain protein [Medicago
           truncatula]
          Length = 389

 Score = 87.0 bits (214), Expect = 7e-18
 Identities = 52/109 (47%), Positives = 63/109 (57%), Gaps = 5/109 (4%)
 Frame = +1

Query: 7   YGFGYDRLSDNYKVVAFSCYRVG---GSFNWVCK-KVEVHALGTNTWKTIQGLPPAFPFG 174
           +GFGYD   +NYKV+    Y +    GS NWV K +V+VH LG+N WK I      FPFG
Sbjct: 176 FGFGYDSFKENYKVIVVLHYLIRDSTGSDNWVHKSEVKVHTLGSNIWKNIH----EFPFG 231

Query: 175 L-TVRERGIFLSGTINWLGITFSPIKMRVIFSFDLRNESYQELLPPDDG 318
           +  V   G F+SGTINWL         R I SFDL  ESYQ++ PP  G
Sbjct: 232 VFPVARSGKFVSGTINWLASRQFHPCTRSIVSFDLAKESYQKISPPSYG 280


>ref|XP_019420066.1| PREDICTED: F-box/kelch-repeat protein At3g23880-like [Lupinus
           angustifolius]
 gb|OIV95875.1| hypothetical protein TanjilG_06851 [Lupinus angustifolius]
          Length = 367

 Score = 86.7 bits (213), Expect = 8e-18
 Identities = 52/108 (48%), Positives = 58/108 (53%), Gaps = 2/108 (1%)
 Frame = +1

Query: 1   TIYGFGYDRLSDNYKVVAFSCYRVG-GSFNWVCKKVEVHALGTNTWKTIQGLPPAFPFGL 177
           TIYGFGYD LSD YKVV   CY  G G       +V+VH LGT+ W+ IQ  P   PF  
Sbjct: 151 TIYGFGYDCLSDTYKVVGVFCYECGSGGAIAYRTEVKVHTLGTDYWRRIQEFPSGVPFD- 209

Query: 178 TVRERGIFLSGTINWLGITFSPIKMR-VIFSFDLRNESYQELLPPDDG 318
                G F+ G INWL           VI S DL  ESY+ELL PD G
Sbjct: 210 ---SSGKFVCGAINWLASGSDAFNSSWVIVSLDLGKESYEELLQPDYG 254