BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00015191
         (2430 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_012570069.1| PREDICTED: pullulanase 1, chloroplastic [Cic...  1258   0.0  
gb|KYP60685.1| hypothetical protein KK1_023096 [Cajanus cajan]       1248   0.0  
ref|XP_020222320.1| pullulanase 1, chloroplastic [Cajanus cajan]     1247   0.0  
ref|XP_013469271.1| alpha-1,6-glucosidase, pullulanase-type prot...  1245   0.0  
gb|KHN37887.1| Pullulanase 1, chloroplastic [Glycine soja]           1242   0.0  
ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic isof...  1242   0.0  
ref|XP_017409401.1| PREDICTED: pullulanase 1, chloroplastic isof...  1221   0.0  
ref|XP_016175196.1| pullulanase 1, chloroplastic [Arachis ipaensis]  1221   0.0  
gb|KOM28793.1| hypothetical protein LR48_Vigan588s000100 [Vigna ...  1221   0.0  
ref|XP_022641088.1| pullulanase 1, chloroplastic isoform X3 [Vig...  1215   0.0  
ref|XP_014513519.1| pullulanase 1, chloroplastic isoform X1 [Vig...  1215   0.0  
ref|XP_017409404.1| PREDICTED: pullulanase 1, chloroplastic isof...  1212   0.0  
ref|XP_017409399.1| PREDICTED: pullulanase 1, chloroplastic isof...  1212   0.0  
ref|XP_014513520.1| pullulanase 1, chloroplastic isoform X2 [Vig...  1210   0.0  
ref|XP_019461050.1| PREDICTED: pullulanase 1, chloroplastic [Lup...  1206   0.0  
ref|XP_017409400.1| PREDICTED: pullulanase 1, chloroplastic isof...  1204   0.0  
gb|OIW01726.1| hypothetical protein TanjilG_03864 [Lupinus angus...  1202   0.0  
ref|XP_015941142.1| pullulanase 1, chloroplastic [Arachis durane...  1197   0.0  
ref|XP_017409405.1| PREDICTED: pullulanase 1, chloroplastic isof...  1176   0.0  
ref|XP_017409403.1| PREDICTED: pullulanase 1, chloroplastic isof...  1154   0.0  

>ref|XP_012570069.1| PREDICTED: pullulanase 1, chloroplastic [Cicer arietinum]
          Length = 953

 Score = 1258 bits (3254), Expect = 0.0
 Identities = 609/706 (86%), Positives = 648/706 (91%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGFCYLLASKSASLTIANCKIQGED 491
            V+++AS SQM+   LLYSR YWV+E VIAWNV+  NGFCYLL+SK ASL+I+N +IQGED
Sbjct: 59   VKESASFSQMENSLLLYSRAYWVSEFVIAWNVDVENGFCYLLSSKYASLSISNGQIQGED 118

Query: 492  LKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNCT 671
            LKIKL ED+AGL  NVVEKFPHIRGYRAF+LPP   VKS LKSQLAVV  DSDE CRNCT
Sbjct: 119  LKIKLEEDKAGLSANVVEKFPHIRGYRAFRLPPGSDVKSFLKSQLAVVICDSDEKCRNCT 178

Query: 672  GLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLEE 851
            GLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V+AYIYKHPSGDDP+EIV LEE
Sbjct: 179  GLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQLVHAYIYKHPSGDDPIEIVPLEE 238

Query: 852  EHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDSN 1031
            EHGVWRTKGPKSWEG          HPST RVEKCY NDPYARGLSADG+RTFLL+LDS+
Sbjct: 239  EHGVWRTKGPKSWEGCYYVYEVCVYHPSTSRVEKCYANDPYARGLSADGRRTFLLDLDSD 298

Query: 1032 ELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGVL 1211
            ELKPDGWDNLANEKP+L SFSD+SIYEMHIRDFSANDLSVQ +FRGGYLAFT PDS GVL
Sbjct: 299  ELKPDGWDNLANEKPILHSFSDISIYEMHIRDFSANDLSVQTEFRGGYLAFTFPDSAGVL 358

Query: 1212 HLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNLD 1391
            HLKKLSSAGITHVHLLP FQFAGVDDQKENWK VD+S+LE  PPDSDQQQALITAIQN+D
Sbjct: 359  HLKKLSSAGITHVHLLPAFQFAGVDDQKENWKNVDTSVLESFPPDSDQQQALITAIQNID 418

Query: 1392 GYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGPF 1571
            GYNWGYNPVLWGVPKGSYASN NGP RA+EFRKMVQALN  GLR+VLD VYNHLQGSGPF
Sbjct: 419  GYNWGYNPVLWGVPKGSYASNPNGPNRAVEFRKMVQALNRTGLRIVLDVVYNHLQGSGPF 478

Query: 1572 DEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGFR 1751
            DEHSVLDKIVPGYYLRR+ DGFIE+STCMNNTASEHFMVERLILDDLVHWAVNYKIDGFR
Sbjct: 479  DEHSVLDKIVPGYYLRRNTDGFIENSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGFR 538

Query: 1752 FDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNL 1931
            FDLMGHIMKSTMVKAKNALHCLTK+KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNL
Sbjct: 539  FDLMGHIMKSTMVKAKNALHCLTKEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNL 598

Query: 1932 CGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDHI 2111
             GT IG+FNDRIRDA+LGGSPFGHPLQQGFVTGL LQPN HDHGTEA MK ML  SMDHI
Sbjct: 599  SGTHIGTFNDRIRDAMLGGSPFGHPLQQGFVTGLLLQPNDHDHGTEADMKSMLAASMDHI 658

Query: 2112 QIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIVS 2291
            QIGMAANLQDFVLTNSKGEEVKGSE+LTY GTPVA+A+CPIET+NYVSAHDNETLFDIVS
Sbjct: 659  QIGMAANLQDFVLTNSKGEEVKGSEVLTYGGTPVAFASCPIETVNYVSAHDNETLFDIVS 718

Query: 2292 LKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            LKTPMDI++AERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR
Sbjct: 719  LKTPMDISVAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 764


>gb|KYP60685.1| hypothetical protein KK1_023096 [Cajanus cajan]
          Length = 922

 Score = 1248 bits (3228), Expect = 0.0
 Identities = 608/707 (85%), Positives = 650/707 (91%), Gaps = 1/707 (0%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            +EQ+ S SQMQ  S LYSR YWVT+S+IAWNV+ GN   CYLLASK+ASLTIANC+IQGE
Sbjct: 60   LEQSTSFSQMQH-SFLYSRAYWVTDSLIAWNVDVGNELSCYLLASKNASLTIANCQIQGE 118

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLKIKL ED AGLP NVVEKFPHIRGY+AFKLPP   VK LLKSQLAVV YDSDE CRNC
Sbjct: 119  DLKIKLQEDTAGLPANVVEKFPHIRGYKAFKLPPALDVKPLLKSQLAVVIYDSDEKCRNC 178

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            +GLQLPGVLDE+FSYSGPLGALFSE+ VSLYLWAPTAQ V+AYIYK+PSGDDP EIV LE
Sbjct: 179  SGLQLPGVLDELFSYSGPLGALFSEEAVSLYLWAPTAQAVHAYIYKNPSGDDPTEIVCLE 238

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           HPSTLRVEKCY NDPYARGLS+DGKRTFLLNLD+
Sbjct: 239  EENGVWRTKGPKSWERCYYVYEVCVYHPSTLRVEKCYANDPYARGLSSDGKRTFLLNLDT 298

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            ++LKP GW+NLANEKPV+ SFSD+SIYEMHIRDFSA+DLSVQP+FRGGYLAFTL DS GV
Sbjct: 299  DDLKPGGWNNLANEKPVIHSFSDISIYEMHIRDFSASDLSVQPEFRGGYLAFTLLDSAGV 358

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
             HLK+LSSAGITH+HLLPTF FAGVDDQKENW+FVD++ILE LPPDSDQQQALITAIQN 
Sbjct: 359  HHLKRLSSAGITHIHLLPTFHFAGVDDQKENWRFVDTTILESLPPDSDQQQALITAIQNF 418

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSYASN NGPYR IEFR+MV+ALNHIGLRVVLD VYNHLQGSGP
Sbjct: 419  DGYNWGYNPVLWGVPKGSYASNPNGPYRTIEFREMVKALNHIGLRVVLDVVYNHLQGSGP 478

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR+ DGFIEHSTC+NNTASEHFMVERLI+DDLVHWAVNYKIDGF
Sbjct: 479  FDEHSVLDKIVPGYYLRRNTDGFIEHSTCINNTASEHFMVERLIIDDLVHWAVNYKIDGF 538

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMKSTMVKAK AL+ LTK+KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 539  RFDLMGHIMKSTMVKAKTALNFLTKEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 598

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDH 2108
            L  TQIGSFNDRIRDAILGGSPFGHPLQQGFVTGL +QPNGHDHGTEA  K ML  SMDH
Sbjct: 599  LPRTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLMQPNGHDHGTEANAKSMLAASMDH 658

Query: 2109 IQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIV 2288
            IQIGMAANLQDFVLTNS+GEEVKGSEILTYDGTPVAYA+CPIETINYVSAHDNETLFDIV
Sbjct: 659  IQIGMAANLQDFVLTNSEGEEVKGSEILTYDGTPVAYASCPIETINYVSAHDNETLFDIV 718

Query: 2289 SLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SLKTPMD+T++ERCRINHLATS+IALSQGIPFFHSGDEILRSKSLDR
Sbjct: 719  SLKTPMDLTVSERCRINHLATSIIALSQGIPFFHSGDEILRSKSLDR 765


>ref|XP_020222320.1| pullulanase 1, chloroplastic [Cajanus cajan]
          Length = 958

 Score = 1247 bits (3226), Expect = 0.0
 Identities = 608/708 (85%), Positives = 650/708 (91%), Gaps = 2/708 (0%)
 Frame = +3

Query: 312  VEQTASISQMQ-QDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQG 485
            +EQ+ S SQ Q Q S LYSR YWVT+S+IAWNV+ GN   CYLLASK+ASLTIANC+IQG
Sbjct: 60   LEQSTSFSQTQMQHSFLYSRAYWVTDSLIAWNVDVGNELSCYLLASKNASLTIANCQIQG 119

Query: 486  EDLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRN 665
            EDLKIKL ED AGLP NVVEKFPHIRGY+AFKLPP   VK LLKSQLAVV YDSDE CRN
Sbjct: 120  EDLKIKLQEDTAGLPANVVEKFPHIRGYKAFKLPPALDVKPLLKSQLAVVIYDSDEKCRN 179

Query: 666  CTGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSL 845
            C+GLQLPGVLDE+FSYSGPLGALFSE+ VSLYLWAPTAQ V+AYIYK+PSGDDP EIV L
Sbjct: 180  CSGLQLPGVLDELFSYSGPLGALFSEEAVSLYLWAPTAQAVHAYIYKNPSGDDPTEIVCL 239

Query: 846  EEEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLD 1025
            EEE+GVWRTKGPKSWE           HPSTLRVEKCY NDPYARGLS+DGKRTFLLNLD
Sbjct: 240  EEENGVWRTKGPKSWERCYYVYEVCVYHPSTLRVEKCYANDPYARGLSSDGKRTFLLNLD 299

Query: 1026 SNELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTG 1205
            +++LKP GW+NLANEKPV+ SFSD+SIYEMHIRDFSA+DLSVQP+FRGGYLAFTL DS G
Sbjct: 300  TDDLKPGGWNNLANEKPVIHSFSDISIYEMHIRDFSASDLSVQPEFRGGYLAFTLLDSAG 359

Query: 1206 VLHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQN 1385
            V HLK+LSSAGITH+HLLPTF FAGVDDQKENW+FVD++ILE LPPDSDQQQALITAIQN
Sbjct: 360  VHHLKRLSSAGITHIHLLPTFHFAGVDDQKENWRFVDTTILESLPPDSDQQQALITAIQN 419

Query: 1386 LDGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSG 1565
             DGYNWGYNPVLWGVPKGSYASN NGPYR IEFR+MV+ALNHIGLRVVLD VYNHLQGSG
Sbjct: 420  FDGYNWGYNPVLWGVPKGSYASNPNGPYRTIEFREMVKALNHIGLRVVLDVVYNHLQGSG 479

Query: 1566 PFDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDG 1745
            PFDEHSVLDKIVPGYYLRR+ DGFIEHSTC+NNTASEHFMVERLI+DDLVHWAVNYKIDG
Sbjct: 480  PFDEHSVLDKIVPGYYLRRNTDGFIEHSTCINNTASEHFMVERLIIDDLVHWAVNYKIDG 539

Query: 1746 FRFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQF 1925
            FRFDLMGHIMKSTMVKAK AL+ LTK+KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQF
Sbjct: 540  FRFDLMGHIMKSTMVKAKTALNFLTKEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQF 599

Query: 1926 NLCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMD 2105
            NL  TQIGSFNDRIRDAILGGSPFGHPLQQGFVTGL +QPNGHDHGTEA  K ML  SMD
Sbjct: 600  NLPRTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLMQPNGHDHGTEANAKSMLAASMD 659

Query: 2106 HIQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDI 2285
            HIQIGMAANLQDFVLTNS+GEEVKGSEILTYDGTPVAYA+CPIETINYVSAHDNETLFDI
Sbjct: 660  HIQIGMAANLQDFVLTNSEGEEVKGSEILTYDGTPVAYASCPIETINYVSAHDNETLFDI 719

Query: 2286 VSLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            VSLKTPMD+T++ERCRINHLATS+IALSQGIPFFHSGDEILRSKSLDR
Sbjct: 720  VSLKTPMDLTVSERCRINHLATSIIALSQGIPFFHSGDEILRSKSLDR 767


>ref|XP_013469271.1| alpha-1,6-glucosidase, pullulanase-type protein [Medicago truncatula]
 gb|KEH43309.1| alpha-1,6-glucosidase, pullulanase-type protein [Medicago truncatula]
          Length = 957

 Score = 1245 bits (3222), Expect = 0.0
 Identities = 603/707 (85%), Positives = 648/707 (91%), Gaps = 1/707 (0%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDG-NGFCYLLASKSASLTIANCKIQGE 488
            V+QT SISQMQ + LLYSR YW+T+S+IAWNV+   NG CYLL+SK ASL I+N +IQGE
Sbjct: 62   VDQTTSISQMQDNLLLYSRAYWLTQSLIAWNVDHHQNGICYLLSSKDASLNISNSQIQGE 121

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLKIKL ED+AGLP NVVEKFPHIRGY+AF LPP   +KSLLKSQLAVV YDSDE CR+C
Sbjct: 122  DLKIKLQEDKAGLPANVVEKFPHIRGYKAFNLPPGSDIKSLLKSQLAVVIYDSDEKCRDC 181

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ+V AYIYKHPSGDDP+EIV LE
Sbjct: 182  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQSVRAYIYKHPSGDDPIEIVPLE 241

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EEHGVWRTKGPKSWEG          HPST RVEKCY NDPYARGLS+DG+RTFLLNLDS
Sbjct: 242  EEHGVWRTKGPKSWEGCYYVYEVCVYHPSTSRVEKCYANDPYARGLSSDGRRTFLLNLDS 301

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            NELKPD W+NLA+EKP+L SFSD+SIYE+HIRDFSANDLSVQP+FRGGYLAFTL DS GV
Sbjct: 302  NELKPDRWENLADEKPILHSFSDISIYELHIRDFSANDLSVQPEFRGGYLAFTLTDSAGV 361

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLKKLSSAGITHVHLLPTFQFAGVDDQKENW+ VD+S+LE  PPDSDQQQALITAIQN 
Sbjct: 362  LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWRNVDTSVLESFPPDSDQQQALITAIQNF 421

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSYASN NGP R IEFRKMVQAL+HIGLRVVLDTVYNHLQG+GP
Sbjct: 422  DGYNWGYNPVLWGVPKGSYASNPNGPNRTIEFRKMVQALSHIGLRVVLDTVYNHLQGNGP 481

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR+ DGFIE+STCMNNTASEH MVERLILDDL+HWAVNYK+DGF
Sbjct: 482  FDEHSVLDKIVPGYYLRRNTDGFIENSTCMNNTASEHSMVERLILDDLLHWAVNYKVDGF 541

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMKSTMVKAKNAL  LTK+KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 542  RFDLMGHIMKSTMVKAKNALQRLTKEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 601

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDH 2108
            L GTQIGSFNDRIRD++LGGSPFGHPLQQGFVTGL LQPNGHDHGTEA MK ML  SMDH
Sbjct: 602  LAGTQIGSFNDRIRDSVLGGSPFGHPLQQGFVTGLLLQPNGHDHGTEANMKSMLAASMDH 661

Query: 2109 IQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIV 2288
            IQIGMA NL+DFVLTNS+GEEVKGSE+LTY GTPVA+A+ PIET+NYVSAHDNETLFDIV
Sbjct: 662  IQIGMAGNLKDFVLTNSEGEEVKGSEVLTYGGTPVAFASYPIETVNYVSAHDNETLFDIV 721

Query: 2289 SLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SLKTPMDI +AERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR
Sbjct: 722  SLKTPMDIGVAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 768


>gb|KHN37887.1| Pullulanase 1, chloroplastic [Glycine soja]
          Length = 954

 Score = 1242 bits (3214), Expect = 0.0
 Identities = 604/707 (85%), Positives = 647/707 (91%), Gaps = 1/707 (0%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ+ S SQMQ + LLYSR YWV ES+IAW+V+ GNGF CYLLASK+ASLTIANC+IQGE
Sbjct: 60   VEQSVSSSQMQ-NGLLYSRAYWVAESLIAWDVDVGNGFSCYLLASKNASLTIANCQIQGE 118

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLKIKL EDR GLP NVVEKFPHIRGY+ F LPP   VK LLK QLAVV  DSD  C NC
Sbjct: 119  DLKIKLQEDRVGLPANVVEKFPHIRGYKVFSLPPTLDVKPLLKFQLAVVICDSDGECMNC 178

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLD++FSYSGPLGALFSE+ VSLYLWAPTAQ V+AYIYK PSGDDP+EIV LE
Sbjct: 179  TGLQLPGVLDDLFSYSGPLGALFSEEAVSLYLWAPTAQAVHAYIYKDPSGDDPIEIVCLE 238

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWEG          HPST+++EKCY +DPYARGLS+DG+R+FLLNLDS
Sbjct: 239  EENGVWRTKGPKSWEGCYYVYEVCVYHPSTMQIEKCYTSDPYARGLSSDGRRSFLLNLDS 298

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
             +LKPDGWDNLAN+KP + SFSD+SIYEMHIRDFSA+DLSVQP+FRGGYLAFTL DS GV
Sbjct: 299  VKLKPDGWDNLANKKPTIHSFSDISIYEMHIRDFSASDLSVQPEFRGGYLAFTLQDSAGV 358

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLKKLSSAGITHVHLLPTFQFAGVDDQKE+W+FVD+SILE LPPDSDQQQALITAIQN 
Sbjct: 359  LHLKKLSSAGITHVHLLPTFQFAGVDDQKEDWRFVDTSILESLPPDSDQQQALITAIQNF 418

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSYASN NGPYR IEFRKMV ALNHIGLRVVLD VYNHLQGSGP
Sbjct: 419  DGYNWGYNPVLWGVPKGSYASNPNGPYRTIEFRKMVMALNHIGLRVVLDVVYNHLQGSGP 478

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR++DG IEHSTC+NNTASEHFMVERLILDDLVHWAVNYKIDGF
Sbjct: 479  FDEHSVLDKIVPGYYLRRNSDGLIEHSTCINNTASEHFMVERLILDDLVHWAVNYKIDGF 538

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMKSTMVKAK AL CLTK+KDG+DGSSIYIYGEGWDFGEVAKNGRG+NASQFN
Sbjct: 539  RFDLMGHIMKSTMVKAKTALQCLTKEKDGLDGSSIYIYGEGWDFGEVAKNGRGVNASQFN 598

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDH 2108
            L GTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGL LQPNGHDHGTEA  K ML  SMDH
Sbjct: 599  LPGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLLQPNGHDHGTEANAKSMLAASMDH 658

Query: 2109 IQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIV 2288
            IQIGMAANL+DFVLTNS+GEEVKGSEILTY GTPVAYA+CPIETINYVSAHDNETLFDIV
Sbjct: 659  IQIGMAANLKDFVLTNSEGEEVKGSEILTYGGTPVAYASCPIETINYVSAHDNETLFDIV 718

Query: 2289 SLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SLKTPMDI+++ERCRINHLATS+IALSQGIPFFHSGDEILRSKSLDR
Sbjct: 719  SLKTPMDISVSERCRINHLATSIIALSQGIPFFHSGDEILRSKSLDR 765


>ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Glycine max]
 gb|KRH34652.1| hypothetical protein GLYMA_10G197000 [Glycine max]
          Length = 951

 Score = 1242 bits (3213), Expect = 0.0
 Identities = 604/707 (85%), Positives = 648/707 (91%), Gaps = 1/707 (0%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ+AS SQMQ + LLYSR YWV ES+IAW+V+ GNGF CYLLASK+ASLTIANC+IQGE
Sbjct: 57   VEQSASSSQMQ-NGLLYSRAYWVAESLIAWDVDVGNGFSCYLLASKNASLTIANCQIQGE 115

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLKIKL EDR GLP NVVEKFPHIRG++ F LPP   VK LLK +LAVV  DSD  C NC
Sbjct: 116  DLKIKLQEDRVGLPANVVEKFPHIRGHKVFSLPPTLDVKPLLKFRLAVVICDSDGECMNC 175

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLD++FSYSGPLGALFSE+ VSLYLWAPTAQ V+AYIYK PSGDDP+EIV LE
Sbjct: 176  TGLQLPGVLDDLFSYSGPLGALFSEEAVSLYLWAPTAQAVHAYIYKDPSGDDPIEIVCLE 235

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWEG          HPST+R+EKCY +DPYARGLS+DG+R+FLLNLDS
Sbjct: 236  EENGVWRTKGPKSWEGCYYVYEVCVYHPSTMRIEKCYTSDPYARGLSSDGRRSFLLNLDS 295

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
             +LKPDGWDNLAN+KP + SFSD+SIYEMHIRDFSA+DLSVQP+FRGGYLAFTL DS GV
Sbjct: 296  VKLKPDGWDNLANKKPTIHSFSDISIYEMHIRDFSASDLSVQPEFRGGYLAFTLQDSAGV 355

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLKKLSSAGITHVHLLPTFQFAGVDDQKE+W+FVD+SILE LPPDSDQQQALITAIQN 
Sbjct: 356  LHLKKLSSAGITHVHLLPTFQFAGVDDQKEDWRFVDTSILESLPPDSDQQQALITAIQNF 415

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSYASN NGPYR IEFRKMV ALNHIGLRVVLD VYNHLQGSGP
Sbjct: 416  DGYNWGYNPVLWGVPKGSYASNPNGPYRTIEFRKMVMALNHIGLRVVLDVVYNHLQGSGP 475

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR++DG IEHSTC+NNTASEHFMVERLILDDLVHWAVNYKIDGF
Sbjct: 476  FDEHSVLDKIVPGYYLRRNSDGLIEHSTCINNTASEHFMVERLILDDLVHWAVNYKIDGF 535

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMKSTMVKAK AL CLTK+KDG+DGSSIYIYGEGWDFGEVAKNGRG+NASQFN
Sbjct: 536  RFDLMGHIMKSTMVKAKTALQCLTKEKDGLDGSSIYIYGEGWDFGEVAKNGRGVNASQFN 595

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDH 2108
            L GTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGL LQPNGHDHGTEA  K ML  SMDH
Sbjct: 596  LPGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLLQPNGHDHGTEANAKSMLAASMDH 655

Query: 2109 IQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIV 2288
            IQIGMAANL+DFVLTNS+GEEVKGSEILTY GTPVAYA+CPIETINYVSAHDNETLFDIV
Sbjct: 656  IQIGMAANLKDFVLTNSEGEEVKGSEILTYGGTPVAYASCPIETINYVSAHDNETLFDIV 715

Query: 2289 SLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SLKTPMDI+++ERCRINHLATS+IALSQGIPFFHSGDEILRSKSLDR
Sbjct: 716  SLKTPMDISVSERCRINHLATSIIALSQGIPFFHSGDEILRSKSLDR 762


>ref|XP_017409401.1| PREDICTED: pullulanase 1, chloroplastic isoform X3 [Vigna angularis]
          Length = 971

 Score = 1221 bits (3160), Expect = 0.0
 Identities = 596/707 (84%), Positives = 639/707 (90%), Gaps = 1/707 (0%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ+AS+SQMQ +SLLYSR YWVTE +IAWNV+  N F C L+ASK+ASLTI+N +IQGE
Sbjct: 77   VEQSASLSQMQ-NSLLYSRAYWVTEFLIAWNVDVANEFSCQLIASKNASLTISNGQIQGE 135

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLK++L EDRAGLP NV++KFPHIRGY+AFKLP    VK LLKSQLAV  YDSD+ CRNC
Sbjct: 136  DLKVELQEDRAGLPANVLQKFPHIRGYKAFKLPSTLDVKPLLKSQLAVAIYDSDDKCRNC 195

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V AYIYK PSGDDP+EIV LE
Sbjct: 196  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQAVRAYIYKDPSGDDPIEIVCLE 255

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           H STLRVEKCY NDPYARG+S+DG+RTFLLNLDS
Sbjct: 256  EENGVWRTKGPKSWEHCYYVYEVCVYHHSTLRVEKCYANDPYARGVSSDGQRTFLLNLDS 315

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            +ELKPDGWD+LANEKP +  F+D+SIYEMHIRDFSA DLSVQP FRGGYLAFTL DS GV
Sbjct: 316  DELKPDGWDDLANEKPTIHCFTDISIYEMHIRDFSATDLSVQPAFRGGYLAFTLQDSAGV 375

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLK+LSSAGITHVHLLPTFQFAGVDDQKENW+FVD+SILE  PPDSDQQQALITAIQN 
Sbjct: 376  LHLKRLSSAGITHVHLLPTFQFAGVDDQKENWRFVDTSILESFPPDSDQQQALITAIQNF 435

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSY+SN NGPYR IEFRKMV+ALNH+GLRVVLD VYNHLQGSGP
Sbjct: 436  DGYNWGYNPVLWGVPKGSYSSNPNGPYRTIEFRKMVKALNHMGLRVVLDVVYNHLQGSGP 495

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR+ DGFIE+STC+NNTASEHFMVERLILDDLVHWA+NYKIDGF
Sbjct: 496  FDEHSVLDKIVPGYYLRRNRDGFIENSTCINNTASEHFMVERLILDDLVHWALNYKIDGF 555

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMK TMV+AK ALHCLT +KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 556  RFDLMGHIMKRTMVQAKTALHCLTTEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 615

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDH 2108
            L GTQIGSFNDRIRDAILGGSPFG PLQQGFVTGL LQPN HDHGTE   K ML  SMDH
Sbjct: 616  LPGTQIGSFNDRIRDAILGGSPFGDPLQQGFVTGLLLQPNDHDHGTEENAKSMLAASMDH 675

Query: 2109 IQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIV 2288
            IQIGMAANLQDFVLTN KGEEVKGSEILTYDGTPVAYA+CP ETINYVSAHDNETLFDIV
Sbjct: 676  IQIGMAANLQDFVLTNCKGEEVKGSEILTYDGTPVAYASCPTETINYVSAHDNETLFDIV 735

Query: 2289 SLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SLKTPMDIT+ ERCRINHLATS+IALSQGIPFFH GDEILRSKSLDR
Sbjct: 736  SLKTPMDITVTERCRINHLATSIIALSQGIPFFHCGDEILRSKSLDR 782


>ref|XP_016175196.1| pullulanase 1, chloroplastic [Arachis ipaensis]
          Length = 961

 Score = 1221 bits (3160), Expect = 0.0
 Identities = 590/706 (83%), Positives = 642/706 (90%), Gaps = 1/706 (0%)
 Frame = +3

Query: 315  EQTASISQMQQDSLLYSRGYWVTESVIAWNVED-GNGFCYLLASKSASLTIANCKIQGED 491
            +Q+ S SQMQ DSLLYSR YWVTES+IAWN++   NGFC LL SK AS TI++ +IQGED
Sbjct: 67   QQSLSFSQMQNDSLLYSRAYWVTESIIAWNIDIVENGFCSLLTSKDASFTISDNQIQGED 126

Query: 492  LKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNCT 671
            +KIKL EDR+GLP  VVEKFPHIRGY+AFKLPP   VKSLLKSQLAV  YDSDE CRNCT
Sbjct: 127  VKIKLQEDRSGLPAKVVEKFPHIRGYKAFKLPPDSDVKSLLKSQLAVAVYDSDEKCRNCT 186

Query: 672  GLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLEE 851
            GLQLPGVLDE+FSY+GPLGAL+S++ VSL LWAPTAQ VNA+IYK+PSGDDP+E+V LEE
Sbjct: 187  GLQLPGVLDELFSYNGPLGALYSDEAVSLSLWAPTAQAVNAHIYKYPSGDDPIEVVCLEE 246

Query: 852  EHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDSN 1031
            E GVWR KGPKSWEG          HPSTLRVEKCY NDPYARGLSADG+RTFL+N+DS+
Sbjct: 247  EDGVWRIKGPKSWEGCYYVYEVCVYHPSTLRVEKCYANDPYARGLSADGRRTFLINIDSD 306

Query: 1032 ELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGVL 1211
            ELKPDGWDNLA+EKPVL SFSD+SIYEMHIRDFSANDLSV+P+FRGGYLAFTL DS GVL
Sbjct: 307  ELKPDGWDNLADEKPVLHSFSDISIYEMHIRDFSANDLSVEPEFRGGYLAFTLLDSAGVL 366

Query: 1212 HLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNLD 1391
            HLKKLSSAGITHVHLLPTFQFAGVDDQKENW++VD+ +LE  PPDSDQQQALITAI++LD
Sbjct: 367  HLKKLSSAGITHVHLLPTFQFAGVDDQKENWRYVDTLVLESFPPDSDQQQALITAIKDLD 426

Query: 1392 GYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGPF 1571
            G+NWGYNPVLWGVPKGSYASN NGPYR  EFRKMVQALNHIGLRVVLD VYNHLQGSGPF
Sbjct: 427  GFNWGYNPVLWGVPKGSYASNPNGPYRTTEFRKMVQALNHIGLRVVLDVVYNHLQGSGPF 486

Query: 1572 DEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGFR 1751
            DEHSVLDKIVPGYYLRR+A+GFIEHSTCMNNTASEHFMVERLI+DDLVHWAVNYK+DGFR
Sbjct: 487  DEHSVLDKIVPGYYLRRNANGFIEHSTCMNNTASEHFMVERLIVDDLVHWAVNYKVDGFR 546

Query: 1752 FDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNL 1931
            FDLMGHIMKSTMVKAK+AL  LTK KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNL
Sbjct: 547  FDLMGHIMKSTMVKAKDALLSLTKQKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNL 606

Query: 1932 CGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDHI 2111
              T IGSFNDRIRDA+LGGSPFGHPLQQGF TGL LQPNGHDHGT+  M+ ML TS DHI
Sbjct: 607  SQTGIGSFNDRIRDAVLGGSPFGHPLQQGFATGLLLQPNGHDHGTQENMESMLATSKDHI 666

Query: 2112 QIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIVS 2291
            QIGMAANL+DF+LTNS G+EVKGSEILT+DG+PVAYA  PIETINYVSAHDNETLFDIVS
Sbjct: 667  QIGMAANLRDFMLTNSDGKEVKGSEILTHDGSPVAYALSPIETINYVSAHDNETLFDIVS 726

Query: 2292 LKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            LKTPMDIT+A+RCRINHLATS+IALSQGIPFFH+GDEILRSKSLDR
Sbjct: 727  LKTPMDITVADRCRINHLATSIIALSQGIPFFHAGDEILRSKSLDR 772


>gb|KOM28793.1| hypothetical protein LR48_Vigan588s000100 [Vigna angularis]
          Length = 957

 Score = 1221 bits (3160), Expect = 0.0
 Identities = 596/707 (84%), Positives = 639/707 (90%), Gaps = 1/707 (0%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ+AS+SQMQ +SLLYSR YWVTE +IAWNV+  N F C L+ASK+ASLTI+N +IQGE
Sbjct: 63   VEQSASLSQMQ-NSLLYSRAYWVTEFLIAWNVDVANEFSCQLIASKNASLTISNGQIQGE 121

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLK++L EDRAGLP NV++KFPHIRGY+AFKLP    VK LLKSQLAV  YDSD+ CRNC
Sbjct: 122  DLKVELQEDRAGLPANVLQKFPHIRGYKAFKLPSTLDVKPLLKSQLAVAIYDSDDKCRNC 181

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V AYIYK PSGDDP+EIV LE
Sbjct: 182  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQAVRAYIYKDPSGDDPIEIVCLE 241

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           H STLRVEKCY NDPYARG+S+DG+RTFLLNLDS
Sbjct: 242  EENGVWRTKGPKSWEHCYYVYEVCVYHHSTLRVEKCYANDPYARGVSSDGQRTFLLNLDS 301

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            +ELKPDGWD+LANEKP +  F+D+SIYEMHIRDFSA DLSVQP FRGGYLAFTL DS GV
Sbjct: 302  DELKPDGWDDLANEKPTIHCFTDISIYEMHIRDFSATDLSVQPAFRGGYLAFTLQDSAGV 361

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLK+LSSAGITHVHLLPTFQFAGVDDQKENW+FVD+SILE  PPDSDQQQALITAIQN 
Sbjct: 362  LHLKRLSSAGITHVHLLPTFQFAGVDDQKENWRFVDTSILESFPPDSDQQQALITAIQNF 421

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSY+SN NGPYR IEFRKMV+ALNH+GLRVVLD VYNHLQGSGP
Sbjct: 422  DGYNWGYNPVLWGVPKGSYSSNPNGPYRTIEFRKMVKALNHMGLRVVLDVVYNHLQGSGP 481

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR+ DGFIE+STC+NNTASEHFMVERLILDDLVHWA+NYKIDGF
Sbjct: 482  FDEHSVLDKIVPGYYLRRNRDGFIENSTCINNTASEHFMVERLILDDLVHWALNYKIDGF 541

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMK TMV+AK ALHCLT +KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 542  RFDLMGHIMKRTMVQAKTALHCLTTEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 601

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDH 2108
            L GTQIGSFNDRIRDAILGGSPFG PLQQGFVTGL LQPN HDHGTE   K ML  SMDH
Sbjct: 602  LPGTQIGSFNDRIRDAILGGSPFGDPLQQGFVTGLLLQPNDHDHGTEENAKSMLAASMDH 661

Query: 2109 IQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIV 2288
            IQIGMAANLQDFVLTN KGEEVKGSEILTYDGTPVAYA+CP ETINYVSAHDNETLFDIV
Sbjct: 662  IQIGMAANLQDFVLTNCKGEEVKGSEILTYDGTPVAYASCPTETINYVSAHDNETLFDIV 721

Query: 2289 SLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SLKTPMDIT+ ERCRINHLATS+IALSQGIPFFH GDEILRSKSLDR
Sbjct: 722  SLKTPMDITVTERCRINHLATSIIALSQGIPFFHCGDEILRSKSLDR 768


>ref|XP_022641088.1| pullulanase 1, chloroplastic isoform X3 [Vigna radiata var. radiata]
          Length = 862

 Score = 1215 bits (3143), Expect = 0.0
 Identities = 591/707 (83%), Positives = 639/707 (90%), Gaps = 1/707 (0%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ++S+SQMQ +SLLYSR YWVTE +IAWNV+  N F C L+ASK+ASLTIAN +IQGE
Sbjct: 65   VEQSSSLSQMQ-NSLLYSRAYWVTEFLIAWNVDVANEFSCQLIASKNASLTIANGQIQGE 123

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLK++L EDRAGL  NV+EKFPHIRGY+AFKLP    VK LLKSQLAV  YDSD+ CRNC
Sbjct: 124  DLKVELQEDRAGLSANVLEKFPHIRGYKAFKLPSTLDVKPLLKSQLAVAIYDSDDKCRNC 183

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V AYIYK PSGDDP+EIV LE
Sbjct: 184  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQAVRAYIYKDPSGDDPIEIVCLE 243

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           H STLRVEKC+ NDPYARG+S+DG+RTFLLNLDS
Sbjct: 244  EENGVWRTKGPKSWEHCYYVYEVCVYHHSTLRVEKCHANDPYARGVSSDGRRTFLLNLDS 303

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            +ELKPDGWD+LA+EKP +  F+D+S+YEMHIRDFSA DLSVQP FRGGYLAFTL DS GV
Sbjct: 304  DELKPDGWDDLADEKPTIHCFTDISVYEMHIRDFSATDLSVQPAFRGGYLAFTLQDSAGV 363

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLK+LSSAGITHVHLLPTFQFAGVDDQKENW+FVD+SI+E  PPDSDQQQALITAIQN 
Sbjct: 364  LHLKRLSSAGITHVHLLPTFQFAGVDDQKENWRFVDTSIMESFPPDSDQQQALITAIQNF 423

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSYASN NGPYR IEFRKMV+ALNHIGLRVVLD VYNHLQGSGP
Sbjct: 424  DGYNWGYNPVLWGVPKGSYASNPNGPYRTIEFRKMVKALNHIGLRVVLDVVYNHLQGSGP 483

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FD+HSVLDKIVPGYYLRR+ DGFIE+STC+NNTASEHFMVERLILDDLVHWA+NYKIDGF
Sbjct: 484  FDQHSVLDKIVPGYYLRRNRDGFIENSTCINNTASEHFMVERLILDDLVHWALNYKIDGF 543

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMK TMV+AK ALHCLTK+KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 544  RFDLMGHIMKRTMVQAKTALHCLTKEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 603

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDH 2108
            L GTQIGSFNDRIRDAILGGSPFG PLQQGFVTGL LQPN HDHGTE   K ML  SMDH
Sbjct: 604  LPGTQIGSFNDRIRDAILGGSPFGDPLQQGFVTGLLLQPNDHDHGTEENAKSMLAASMDH 663

Query: 2109 IQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIV 2288
            IQIGMAANLQDFVLTN KGEEVKGSEILTYDGTPVAYA+CP ETINYVSAHDNETLFDIV
Sbjct: 664  IQIGMAANLQDFVLTNCKGEEVKGSEILTYDGTPVAYASCPTETINYVSAHDNETLFDIV 723

Query: 2289 SLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SLKTP+DI++ ERCRINHLATS++ALSQGIPFFH GDEILRSKSLDR
Sbjct: 724  SLKTPVDISVTERCRINHLATSIVALSQGIPFFHCGDEILRSKSLDR 770


>ref|XP_014513519.1| pullulanase 1, chloroplastic isoform X1 [Vigna radiata var. radiata]
          Length = 959

 Score = 1215 bits (3143), Expect = 0.0
 Identities = 591/707 (83%), Positives = 639/707 (90%), Gaps = 1/707 (0%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ++S+SQMQ +SLLYSR YWVTE +IAWNV+  N F C L+ASK+ASLTIAN +IQGE
Sbjct: 65   VEQSSSLSQMQ-NSLLYSRAYWVTEFLIAWNVDVANEFSCQLIASKNASLTIANGQIQGE 123

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLK++L EDRAGL  NV+EKFPHIRGY+AFKLP    VK LLKSQLAV  YDSD+ CRNC
Sbjct: 124  DLKVELQEDRAGLSANVLEKFPHIRGYKAFKLPSTLDVKPLLKSQLAVAIYDSDDKCRNC 183

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V AYIYK PSGDDP+EIV LE
Sbjct: 184  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQAVRAYIYKDPSGDDPIEIVCLE 243

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           H STLRVEKC+ NDPYARG+S+DG+RTFLLNLDS
Sbjct: 244  EENGVWRTKGPKSWEHCYYVYEVCVYHHSTLRVEKCHANDPYARGVSSDGRRTFLLNLDS 303

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            +ELKPDGWD+LA+EKP +  F+D+S+YEMHIRDFSA DLSVQP FRGGYLAFTL DS GV
Sbjct: 304  DELKPDGWDDLADEKPTIHCFTDISVYEMHIRDFSATDLSVQPAFRGGYLAFTLQDSAGV 363

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLK+LSSAGITHVHLLPTFQFAGVDDQKENW+FVD+SI+E  PPDSDQQQALITAIQN 
Sbjct: 364  LHLKRLSSAGITHVHLLPTFQFAGVDDQKENWRFVDTSIMESFPPDSDQQQALITAIQNF 423

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSYASN NGPYR IEFRKMV+ALNHIGLRVVLD VYNHLQGSGP
Sbjct: 424  DGYNWGYNPVLWGVPKGSYASNPNGPYRTIEFRKMVKALNHIGLRVVLDVVYNHLQGSGP 483

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FD+HSVLDKIVPGYYLRR+ DGFIE+STC+NNTASEHFMVERLILDDLVHWA+NYKIDGF
Sbjct: 484  FDQHSVLDKIVPGYYLRRNRDGFIENSTCINNTASEHFMVERLILDDLVHWALNYKIDGF 543

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMK TMV+AK ALHCLTK+KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 544  RFDLMGHIMKRTMVQAKTALHCLTKEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 603

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDH 2108
            L GTQIGSFNDRIRDAILGGSPFG PLQQGFVTGL LQPN HDHGTE   K ML  SMDH
Sbjct: 604  LPGTQIGSFNDRIRDAILGGSPFGDPLQQGFVTGLLLQPNDHDHGTEENAKSMLAASMDH 663

Query: 2109 IQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIV 2288
            IQIGMAANLQDFVLTN KGEEVKGSEILTYDGTPVAYA+CP ETINYVSAHDNETLFDIV
Sbjct: 664  IQIGMAANLQDFVLTNCKGEEVKGSEILTYDGTPVAYASCPTETINYVSAHDNETLFDIV 723

Query: 2289 SLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SLKTP+DI++ ERCRINHLATS++ALSQGIPFFH GDEILRSKSLDR
Sbjct: 724  SLKTPVDISVTERCRINHLATSIVALSQGIPFFHCGDEILRSKSLDR 770


>ref|XP_017409404.1| PREDICTED: pullulanase 1, chloroplastic isoform X5 [Vigna angularis]
          Length = 864

 Score = 1212 bits (3137), Expect = 0.0
 Identities = 596/719 (82%), Positives = 639/719 (88%), Gaps = 13/719 (1%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ+AS+SQMQ +SLLYSR YWVTE +IAWNV+  N F C L+ASK+ASLTI+N +IQGE
Sbjct: 77   VEQSASLSQMQ-NSLLYSRAYWVTEFLIAWNVDVANEFSCQLIASKNASLTISNGQIQGE 135

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLK++L EDRAGLP NV++KFPHIRGY+AFKLP    VK LLKSQLAV  YDSD+ CRNC
Sbjct: 136  DLKVELQEDRAGLPANVLQKFPHIRGYKAFKLPSTLDVKPLLKSQLAVAIYDSDDKCRNC 195

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V AYIYK PSGDDP+EIV LE
Sbjct: 196  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQAVRAYIYKDPSGDDPIEIVCLE 255

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           H STLRVEKCY NDPYARG+S+DG+RTFLLNLDS
Sbjct: 256  EENGVWRTKGPKSWEHCYYVYEVCVYHHSTLRVEKCYANDPYARGVSSDGQRTFLLNLDS 315

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            +ELKPDGWD+LANEKP +  F+D+SIYEMHIRDFSA DLSVQP FRGGYLAFTL DS GV
Sbjct: 316  DELKPDGWDDLANEKPTIHCFTDISIYEMHIRDFSATDLSVQPAFRGGYLAFTLQDSAGV 375

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLK+LSSAGITHVHLLPTFQFAGVDDQKENW+FVD+SILE  PPDSDQQQALITAIQN 
Sbjct: 376  LHLKRLSSAGITHVHLLPTFQFAGVDDQKENWRFVDTSILESFPPDSDQQQALITAIQNF 435

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSY+SN NGPYR IEFRKMV+ALNH+GLRVVLD VYNHLQGSGP
Sbjct: 436  DGYNWGYNPVLWGVPKGSYSSNPNGPYRTIEFRKMVKALNHMGLRVVLDVVYNHLQGSGP 495

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR+ DGFIE+STC+NNTASEHFMVERLILDDLVHWA+NYKIDGF
Sbjct: 496  FDEHSVLDKIVPGYYLRRNRDGFIENSTCINNTASEHFMVERLILDDLVHWALNYKIDGF 555

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMK TMV+AK ALHCLT +KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 556  RFDLMGHIMKRTMVQAKTALHCLTTEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 615

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQ------------PNGHDHGTEA 2072
            L GTQIGSFNDRIRDAILGGSPFG PLQQGFVTGL LQ            PN HDHGTE 
Sbjct: 616  LPGTQIGSFNDRIRDAILGGSPFGDPLQQGFVTGLLLQGAGVVLAVCGQRPNDHDHGTEE 675

Query: 2073 GMKYMLTTSMDHIQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYV 2252
              K ML  SMDHIQIGMAANLQDFVLTN KGEEVKGSEILTYDGTPVAYA+CP ETINYV
Sbjct: 676  NAKSMLAASMDHIQIGMAANLQDFVLTNCKGEEVKGSEILTYDGTPVAYASCPTETINYV 735

Query: 2253 SAHDNETLFDIVSLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SAHDNETLFDIVSLKTPMDIT+ ERCRINHLATS+IALSQGIPFFH GDEILRSKSLDR
Sbjct: 736  SAHDNETLFDIVSLKTPMDITVTERCRINHLATSIIALSQGIPFFHCGDEILRSKSLDR 794


>ref|XP_017409399.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Vigna angularis]
          Length = 983

 Score = 1212 bits (3137), Expect = 0.0
 Identities = 596/719 (82%), Positives = 639/719 (88%), Gaps = 13/719 (1%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ+AS+SQMQ +SLLYSR YWVTE +IAWNV+  N F C L+ASK+ASLTI+N +IQGE
Sbjct: 77   VEQSASLSQMQ-NSLLYSRAYWVTEFLIAWNVDVANEFSCQLIASKNASLTISNGQIQGE 135

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLK++L EDRAGLP NV++KFPHIRGY+AFKLP    VK LLKSQLAV  YDSD+ CRNC
Sbjct: 136  DLKVELQEDRAGLPANVLQKFPHIRGYKAFKLPSTLDVKPLLKSQLAVAIYDSDDKCRNC 195

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V AYIYK PSGDDP+EIV LE
Sbjct: 196  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQAVRAYIYKDPSGDDPIEIVCLE 255

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           H STLRVEKCY NDPYARG+S+DG+RTFLLNLDS
Sbjct: 256  EENGVWRTKGPKSWEHCYYVYEVCVYHHSTLRVEKCYANDPYARGVSSDGQRTFLLNLDS 315

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            +ELKPDGWD+LANEKP +  F+D+SIYEMHIRDFSA DLSVQP FRGGYLAFTL DS GV
Sbjct: 316  DELKPDGWDDLANEKPTIHCFTDISIYEMHIRDFSATDLSVQPAFRGGYLAFTLQDSAGV 375

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLK+LSSAGITHVHLLPTFQFAGVDDQKENW+FVD+SILE  PPDSDQQQALITAIQN 
Sbjct: 376  LHLKRLSSAGITHVHLLPTFQFAGVDDQKENWRFVDTSILESFPPDSDQQQALITAIQNF 435

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSY+SN NGPYR IEFRKMV+ALNH+GLRVVLD VYNHLQGSGP
Sbjct: 436  DGYNWGYNPVLWGVPKGSYSSNPNGPYRTIEFRKMVKALNHMGLRVVLDVVYNHLQGSGP 495

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR+ DGFIE+STC+NNTASEHFMVERLILDDLVHWA+NYKIDGF
Sbjct: 496  FDEHSVLDKIVPGYYLRRNRDGFIENSTCINNTASEHFMVERLILDDLVHWALNYKIDGF 555

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMK TMV+AK ALHCLT +KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 556  RFDLMGHIMKRTMVQAKTALHCLTTEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 615

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQ------------PNGHDHGTEA 2072
            L GTQIGSFNDRIRDAILGGSPFG PLQQGFVTGL LQ            PN HDHGTE 
Sbjct: 616  LPGTQIGSFNDRIRDAILGGSPFGDPLQQGFVTGLLLQGAGVVLAVCGQRPNDHDHGTEE 675

Query: 2073 GMKYMLTTSMDHIQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYV 2252
              K ML  SMDHIQIGMAANLQDFVLTN KGEEVKGSEILTYDGTPVAYA+CP ETINYV
Sbjct: 676  NAKSMLAASMDHIQIGMAANLQDFVLTNCKGEEVKGSEILTYDGTPVAYASCPTETINYV 735

Query: 2253 SAHDNETLFDIVSLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SAHDNETLFDIVSLKTPMDIT+ ERCRINHLATS+IALSQGIPFFH GDEILRSKSLDR
Sbjct: 736  SAHDNETLFDIVSLKTPMDITVTERCRINHLATSIIALSQGIPFFHCGDEILRSKSLDR 794


>ref|XP_014513520.1| pullulanase 1, chloroplastic isoform X2 [Vigna radiata var. radiata]
          Length = 957

 Score = 1210 bits (3131), Expect = 0.0
 Identities = 589/707 (83%), Positives = 637/707 (90%), Gaps = 1/707 (0%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ++S+SQ   +SLLYSR YWVTE +IAWNV+  N F C L+ASK+ASLTIAN +IQGE
Sbjct: 65   VEQSSSLSQ---NSLLYSRAYWVTEFLIAWNVDVANEFSCQLIASKNASLTIANGQIQGE 121

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLK++L EDRAGL  NV+EKFPHIRGY+AFKLP    VK LLKSQLAV  YDSD+ CRNC
Sbjct: 122  DLKVELQEDRAGLSANVLEKFPHIRGYKAFKLPSTLDVKPLLKSQLAVAIYDSDDKCRNC 181

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V AYIYK PSGDDP+EIV LE
Sbjct: 182  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQAVRAYIYKDPSGDDPIEIVCLE 241

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           H STLRVEKC+ NDPYARG+S+DG+RTFLLNLDS
Sbjct: 242  EENGVWRTKGPKSWEHCYYVYEVCVYHHSTLRVEKCHANDPYARGVSSDGRRTFLLNLDS 301

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            +ELKPDGWD+LA+EKP +  F+D+S+YEMHIRDFSA DLSVQP FRGGYLAFTL DS GV
Sbjct: 302  DELKPDGWDDLADEKPTIHCFTDISVYEMHIRDFSATDLSVQPAFRGGYLAFTLQDSAGV 361

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLK+LSSAGITHVHLLPTFQFAGVDDQKENW+FVD+SI+E  PPDSDQQQALITAIQN 
Sbjct: 362  LHLKRLSSAGITHVHLLPTFQFAGVDDQKENWRFVDTSIMESFPPDSDQQQALITAIQNF 421

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSYASN NGPYR IEFRKMV+ALNHIGLRVVLD VYNHLQGSGP
Sbjct: 422  DGYNWGYNPVLWGVPKGSYASNPNGPYRTIEFRKMVKALNHIGLRVVLDVVYNHLQGSGP 481

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FD+HSVLDKIVPGYYLRR+ DGFIE+STC+NNTASEHFMVERLILDDLVHWA+NYKIDGF
Sbjct: 482  FDQHSVLDKIVPGYYLRRNRDGFIENSTCINNTASEHFMVERLILDDLVHWALNYKIDGF 541

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMK TMV+AK ALHCLTK+KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 542  RFDLMGHIMKRTMVQAKTALHCLTKEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 601

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDH 2108
            L GTQIGSFNDRIRDAILGGSPFG PLQQGFVTGL LQPN HDHGTE   K ML  SMDH
Sbjct: 602  LPGTQIGSFNDRIRDAILGGSPFGDPLQQGFVTGLLLQPNDHDHGTEENAKSMLAASMDH 661

Query: 2109 IQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIV 2288
            IQIGMAANLQDFVLTN KGEEVKGSEILTYDGTPVAYA+CP ETINYVSAHDNETLFDIV
Sbjct: 662  IQIGMAANLQDFVLTNCKGEEVKGSEILTYDGTPVAYASCPTETINYVSAHDNETLFDIV 721

Query: 2289 SLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SLKTP+DI++ ERCRINHLATS++ALSQGIPFFH GDEILRSKSLDR
Sbjct: 722  SLKTPVDISVTERCRINHLATSIVALSQGIPFFHCGDEILRSKSLDR 768


>ref|XP_019461050.1| PREDICTED: pullulanase 1, chloroplastic [Lupinus angustifolius]
          Length = 963

 Score = 1206 bits (3120), Expect = 0.0
 Identities = 585/707 (82%), Positives = 634/707 (89%), Gaps = 2/707 (0%)
 Frame = +3

Query: 315  EQTASISQMQQDSLLYSRGYWVTESVIAWNVED--GNGFCYLLASKSASLTIANCKIQGE 488
            + + S SQMQ ++LL+SR YWV+ESVIAWNV+D    GFCYLL+S  ASL+I+N +IQGE
Sbjct: 69   QHSVSFSQMQ-NNLLHSRAYWVSESVIAWNVDDVGKEGFCYLLSSNHASLSISNWQIQGE 127

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            D+KIKL EDRAGLP NVVEKFPHIRGYRAF+LPP   VKSLLK+QLAV  YDSD+ CR+C
Sbjct: 128  DVKIKLQEDRAGLPANVVEKFPHIRGYRAFRLPPNLDVKSLLKTQLAVAIYDSDDRCRDC 187

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE++SY+GPLGA +SE+ VSLYLWAPTA  V+AYIYK PSGDDP+EIV LE
Sbjct: 188  TGLQLPGVLDELYSYNGPLGAHYSEEAVSLYLWAPTAHAVHAYIYKDPSGDDPIEIVLLE 247

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EEHGVW TKGPKSWEG          HPSTLRVEKCY NDPYARGLS DG++TFL+NLD 
Sbjct: 248  EEHGVWITKGPKSWEGCYYVYEVTVYHPSTLRVEKCYANDPYARGLSPDGRQTFLVNLDF 307

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            ++LKPDGWDNLANEKPV+ SFSD+SIYEMH+RDFSANDLSVQP+FRGGYLAFTL DS GV
Sbjct: 308  DQLKPDGWDNLANEKPVIHSFSDISIYEMHVRDFSANDLSVQPEFRGGYLAFTLLDSAGV 367

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWK  D+SILE LPPDSDQQQALITAIQN 
Sbjct: 368  LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKSADTSILESLPPDSDQQQALITAIQNF 427

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSYASN NGP R IEFRKMVQ+LN IGLRVVLD VYNHLQGSG 
Sbjct: 428  DGYNWGYNPVLWGVPKGSYASNPNGPCRTIEFRKMVQSLNRIGLRVVLDVVYNHLQGSGL 487

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
             DEHSVLDKIVPGYYLRR+ADGFIE STCMNNTASEH MVERLILDDLVHWAVNYK+DGF
Sbjct: 488  IDEHSVLDKIVPGYYLRRNADGFIEQSTCMNNTASEHIMVERLILDDLVHWAVNYKVDGF 547

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMKSTMVKAKNALHCLTK+KDGVDGSSIYIYGEGWDFGEVAKNGRG+NASQFN
Sbjct: 548  RFDLMGHIMKSTMVKAKNALHCLTKEKDGVDGSSIYIYGEGWDFGEVAKNGRGVNASQFN 607

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDH 2108
            L  T IG FNDRIRDA+LGGSPFGHPLQQGFVTGL LQPNGHDHGTEA  + ML  S DH
Sbjct: 608  LSETGIGCFNDRIRDALLGGSPFGHPLQQGFVTGLLLQPNGHDHGTEANAESMLAASKDH 667

Query: 2109 IQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIV 2288
            IQIGMAANL+DFVLTNS GEE KGSE+L Y GTPVA+A+CP ET+NYVSAHDNETLFDIV
Sbjct: 668  IQIGMAANLRDFVLTNSDGEEAKGSEVLAYGGTPVAFASCPTETVNYVSAHDNETLFDIV 727

Query: 2289 SLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SLKTPMDI++ ERCRINHLATS+IALSQGIPFFHSGDE+LRSKSLDR
Sbjct: 728  SLKTPMDISVDERCRINHLATSIIALSQGIPFFHSGDELLRSKSLDR 774


>ref|XP_017409400.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Vigna angularis]
          Length = 980

 Score = 1204 bits (3116), Expect = 0.0
 Identities = 595/719 (82%), Positives = 636/719 (88%), Gaps = 13/719 (1%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ+AS+SQMQ +SLLYSR YWVTE +IAWNV+  N F C L+ASK+ASLTI+N +IQGE
Sbjct: 77   VEQSASLSQMQ-NSLLYSRAYWVTEFLIAWNVDVANEFSCQLIASKNASLTISNGQIQGE 135

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLK++L EDRAGLP N   KFPHIRGY+AFKLP    VK LLKSQLAV  YDSD+ CRNC
Sbjct: 136  DLKVELQEDRAGLPAN---KFPHIRGYKAFKLPSTLDVKPLLKSQLAVAIYDSDDKCRNC 192

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V AYIYK PSGDDP+EIV LE
Sbjct: 193  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQAVRAYIYKDPSGDDPIEIVCLE 252

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           H STLRVEKCY NDPYARG+S+DG+RTFLLNLDS
Sbjct: 253  EENGVWRTKGPKSWEHCYYVYEVCVYHHSTLRVEKCYANDPYARGVSSDGQRTFLLNLDS 312

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            +ELKPDGWD+LANEKP +  F+D+SIYEMHIRDFSA DLSVQP FRGGYLAFTL DS GV
Sbjct: 313  DELKPDGWDDLANEKPTIHCFTDISIYEMHIRDFSATDLSVQPAFRGGYLAFTLQDSAGV 372

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLK+LSSAGITHVHLLPTFQFAGVDDQKENW+FVD+SILE  PPDSDQQQALITAIQN 
Sbjct: 373  LHLKRLSSAGITHVHLLPTFQFAGVDDQKENWRFVDTSILESFPPDSDQQQALITAIQNF 432

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSY+SN NGPYR IEFRKMV+ALNH+GLRVVLD VYNHLQGSGP
Sbjct: 433  DGYNWGYNPVLWGVPKGSYSSNPNGPYRTIEFRKMVKALNHMGLRVVLDVVYNHLQGSGP 492

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR+ DGFIE+STC+NNTASEHFMVERLILDDLVHWA+NYKIDGF
Sbjct: 493  FDEHSVLDKIVPGYYLRRNRDGFIENSTCINNTASEHFMVERLILDDLVHWALNYKIDGF 552

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMK TMV+AK ALHCLT +KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 553  RFDLMGHIMKRTMVQAKTALHCLTTEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 612

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQ------------PNGHDHGTEA 2072
            L GTQIGSFNDRIRDAILGGSPFG PLQQGFVTGL LQ            PN HDHGTE 
Sbjct: 613  LPGTQIGSFNDRIRDAILGGSPFGDPLQQGFVTGLLLQGAGVVLAVCGQRPNDHDHGTEE 672

Query: 2073 GMKYMLTTSMDHIQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYV 2252
              K ML  SMDHIQIGMAANLQDFVLTN KGEEVKGSEILTYDGTPVAYA+CP ETINYV
Sbjct: 673  NAKSMLAASMDHIQIGMAANLQDFVLTNCKGEEVKGSEILTYDGTPVAYASCPTETINYV 732

Query: 2253 SAHDNETLFDIVSLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SAHDNETLFDIVSLKTPMDIT+ ERCRINHLATS+IALSQGIPFFH GDEILRSKSLDR
Sbjct: 733  SAHDNETLFDIVSLKTPMDITVTERCRINHLATSIIALSQGIPFFHCGDEILRSKSLDR 791


>gb|OIW01726.1| hypothetical protein TanjilG_03864 [Lupinus angustifolius]
          Length = 890

 Score = 1202 bits (3111), Expect = 0.0
 Identities = 581/697 (83%), Positives = 628/697 (90%), Gaps = 2/697 (0%)
 Frame = +3

Query: 345  QDSLLYSRGYWVTESVIAWNVED--GNGFCYLLASKSASLTIANCKIQGEDLKIKLHEDR 518
            Q++LL+SR YWV+ESVIAWNV+D    GFCYLL+S  ASL+I+N +IQGED+KIKL EDR
Sbjct: 5    QNNLLHSRAYWVSESVIAWNVDDVGKEGFCYLLSSNHASLSISNWQIQGEDVKIKLQEDR 64

Query: 519  AGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNCTGLQLPGVLD 698
            AGLP NVVEKFPHIRGYRAF+LPP   VKSLLK+QLAV  YDSD+ CR+CTGLQLPGVLD
Sbjct: 65   AGLPANVVEKFPHIRGYRAFRLPPNLDVKSLLKTQLAVAIYDSDDRCRDCTGLQLPGVLD 124

Query: 699  EIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLEEEHGVWRTKG 878
            E++SY+GPLGA +SE+ VSLYLWAPTA  V+AYIYK PSGDDP+EIV LEEEHGVW TKG
Sbjct: 125  ELYSYNGPLGAHYSEEAVSLYLWAPTAHAVHAYIYKDPSGDDPIEIVLLEEEHGVWITKG 184

Query: 879  PKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDSNELKPDGWDN 1058
            PKSWEG          HPSTLRVEKCY NDPYARGLS DG++TFL+NLD ++LKPDGWDN
Sbjct: 185  PKSWEGCYYVYEVTVYHPSTLRVEKCYANDPYARGLSPDGRQTFLVNLDFDQLKPDGWDN 244

Query: 1059 LANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGVLHLKKLSSAG 1238
            LANEKPV+ SFSD+SIYEMH+RDFSANDLSVQP+FRGGYLAFTL DS GVLHLKKLSSAG
Sbjct: 245  LANEKPVIHSFSDISIYEMHVRDFSANDLSVQPEFRGGYLAFTLLDSAGVLHLKKLSSAG 304

Query: 1239 ITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNLDGYNWGYNPV 1418
            ITHVHLLPTFQFAGVDDQKENWK  D+SILE LPPDSDQQQALITAIQN DGYNWGYNPV
Sbjct: 305  ITHVHLLPTFQFAGVDDQKENWKSADTSILESLPPDSDQQQALITAIQNFDGYNWGYNPV 364

Query: 1419 LWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGPFDEHSVLDKI 1598
            LWGVPKGSYASN NGP R IEFRKMVQ+LN IGLRVVLD VYNHLQGSG  DEHSVLDKI
Sbjct: 365  LWGVPKGSYASNPNGPCRTIEFRKMVQSLNRIGLRVVLDVVYNHLQGSGLIDEHSVLDKI 424

Query: 1599 VPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGFRFDLMGHIMK 1778
            VPGYYLRR+ADGFIE STCMNNTASEH MVERLILDDLVHWAVNYK+DGFRFDLMGHIMK
Sbjct: 425  VPGYYLRRNADGFIEQSTCMNNTASEHIMVERLILDDLVHWAVNYKVDGFRFDLMGHIMK 484

Query: 1779 STMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNLCGTQIGSFN 1958
            STMVKAKNALHCLTK+KDGVDGSSIYIYGEGWDFGEVAKNGRG+NASQFNL  T IG FN
Sbjct: 485  STMVKAKNALHCLTKEKDGVDGSSIYIYGEGWDFGEVAKNGRGVNASQFNLSETGIGCFN 544

Query: 1959 DRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDHIQIGMAANLQ 2138
            DRIRDA+LGGSPFGHPLQQGFVTGL LQPNGHDHGTEA  + ML  S DHIQIGMAANL+
Sbjct: 545  DRIRDALLGGSPFGHPLQQGFVTGLLLQPNGHDHGTEANAESMLAASKDHIQIGMAANLR 604

Query: 2139 DFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIVSLKTPMDITL 2318
            DFVLTNS GEE KGSE+L Y GTPVA+A+CP ET+NYVSAHDNETLFDIVSLKTPMDI++
Sbjct: 605  DFVLTNSDGEEAKGSEVLAYGGTPVAFASCPTETVNYVSAHDNETLFDIVSLKTPMDISV 664

Query: 2319 AERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
             ERCRINHLATS+IALSQGIPFFHSGDE+LRSKSLDR
Sbjct: 665  DERCRINHLATSIIALSQGIPFFHSGDELLRSKSLDR 701


>ref|XP_015941142.1| pullulanase 1, chloroplastic [Arachis duranensis]
          Length = 957

 Score = 1197 bits (3097), Expect = 0.0
 Identities = 581/706 (82%), Positives = 635/706 (89%), Gaps = 1/706 (0%)
 Frame = +3

Query: 315  EQTASISQMQQDSLLYSRGYWVTESVIAWNVED-GNGFCYLLASKSASLTIANCKIQGED 491
            +Q+ S SQMQ DSLLYSR YWVTES+IAWN++   NGFC LLASK AS TI++ +IQGED
Sbjct: 66   QQSLSFSQMQNDSLLYSRAYWVTESIIAWNIDIVENGFCSLLASKDASFTISDNQIQGED 125

Query: 492  LKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNCT 671
            +KIKL EDRAGLP  VVEKFPHIRGY+AFKLPP   VKSLLKSQLAV  YDSDE CRNCT
Sbjct: 126  VKIKLQEDRAGLPAKVVEKFPHIRGYKAFKLPPDSDVKSLLKSQLAVAVYDSDEKCRNCT 185

Query: 672  GLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLEE 851
            GLQLPGVLDE+FSY+GPLGA++S++ VSL LWAPTAQ VNA+IYK+PSGDD +E+V LEE
Sbjct: 186  GLQLPGVLDELFSYNGPLGAIYSDEAVSLSLWAPTAQAVNAHIYKYPSGDDSIEVVCLEE 245

Query: 852  EHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDSN 1031
            E GVWRTKGPKSWEG          HPSTLRVEKCY NDPYARGLSADG+RTFL+N+DS+
Sbjct: 246  EDGVWRTKGPKSWEGCYYVYEVCVYHPSTLRVEKCYANDPYARGLSADGRRTFLINIDSD 305

Query: 1032 ELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGVL 1211
            ELKPDGWDNLA+EKPVL SFSD+SIYEMHIRDFSANDLSV+P+FRGGYLAFTL DS GVL
Sbjct: 306  ELKPDGWDNLADEKPVLHSFSDISIYEMHIRDFSANDLSVEPEFRGGYLAFTLLDSAGVL 365

Query: 1212 HLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNLD 1391
            HLKKLSSAGITHVHLLPTFQFAG+ D       +D+ +LE  PPDSDQQQALITAI++LD
Sbjct: 366  HLKKLSSAGITHVHLLPTFQFAGISDTNY---LIDTLVLESFPPDSDQQQALITAIKDLD 422

Query: 1392 GYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGPF 1571
            G+NWGYNPVLWGVPKGSYASN NGPYR  EFRKMVQALNHIGLRVVLD VYNHLQGSGPF
Sbjct: 423  GFNWGYNPVLWGVPKGSYASNPNGPYRTTEFRKMVQALNHIGLRVVLDVVYNHLQGSGPF 482

Query: 1572 DEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGFR 1751
            DEHSVLDKIVPGYYLRR+A+GFIEHSTCMNNTASEHFMVERLI+DDLVHWAVNYK+DGFR
Sbjct: 483  DEHSVLDKIVPGYYLRRNANGFIEHSTCMNNTASEHFMVERLIVDDLVHWAVNYKVDGFR 542

Query: 1752 FDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNL 1931
            FDLMGHIMKSTMVKAK+AL  LTK  DGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNL
Sbjct: 543  FDLMGHIMKSTMVKAKDALLSLTKQNDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNL 602

Query: 1932 CGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQPNGHDHGTEAGMKYMLTTSMDHI 2111
              T IGSFNDRIRDA+LGGSPFGHPLQQGF TGL LQPNGHDHGT+  M+ ML TS DHI
Sbjct: 603  SQTGIGSFNDRIRDAVLGGSPFGHPLQQGFATGLLLQPNGHDHGTQKNMESMLATSKDHI 662

Query: 2112 QIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYVSAHDNETLFDIVS 2291
            QIGMAANL+DF+LTNS G+EVKGSEILT+DG+PVAYA+ P+ETINYVSAHDNETLFDIVS
Sbjct: 663  QIGMAANLRDFMLTNSDGKEVKGSEILTHDGSPVAYASSPVETINYVSAHDNETLFDIVS 722

Query: 2292 LKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            LKTPMDIT+A+RCRINHLATS+IALSQGIPFFH+GDEILRSKSLDR
Sbjct: 723  LKTPMDITVADRCRINHLATSIIALSQGIPFFHAGDEILRSKSLDR 768


>ref|XP_017409405.1| PREDICTED: pullulanase 1, chloroplastic isoform X6 [Vigna angularis]
          Length = 782

 Score = 1176 bits (3043), Expect = 0.0
 Identities = 578/700 (82%), Positives = 621/700 (88%), Gaps = 13/700 (1%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ+AS+SQMQ +SLLYSR YWVTE +IAWNV+  N F C L+ASK+ASLTI+N +IQGE
Sbjct: 77   VEQSASLSQMQ-NSLLYSRAYWVTEFLIAWNVDVANEFSCQLIASKNASLTISNGQIQGE 135

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLK++L EDRAGLP NV++KFPHIRGY+AFKLP    VK LLKSQLAV  YDSD+ CRNC
Sbjct: 136  DLKVELQEDRAGLPANVLQKFPHIRGYKAFKLPSTLDVKPLLKSQLAVAIYDSDDKCRNC 195

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V AYIYK PSGDDP+EIV LE
Sbjct: 196  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQAVRAYIYKDPSGDDPIEIVCLE 255

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           H STLRVEKCY NDPYARG+S+DG+RTFLLNLDS
Sbjct: 256  EENGVWRTKGPKSWEHCYYVYEVCVYHHSTLRVEKCYANDPYARGVSSDGQRTFLLNLDS 315

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            +ELKPDGWD+LANEKP +  F+D+SIYEMHIRDFSA DLSVQP FRGGYLAFTL DS GV
Sbjct: 316  DELKPDGWDDLANEKPTIHCFTDISIYEMHIRDFSATDLSVQPAFRGGYLAFTLQDSAGV 375

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLK+LSSAGITHVHLLPTFQFAGVDDQKENW+FVD+SILE  PPDSDQQQALITAIQN 
Sbjct: 376  LHLKRLSSAGITHVHLLPTFQFAGVDDQKENWRFVDTSILESFPPDSDQQQALITAIQNF 435

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSY+SN NGPYR IEFRKMV+ALNH+GLRVVLD VYNHLQGSGP
Sbjct: 436  DGYNWGYNPVLWGVPKGSYSSNPNGPYRTIEFRKMVKALNHMGLRVVLDVVYNHLQGSGP 495

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR+ DGFIE+STC+NNTASEHFMVERLILDDLVHWA+NYKIDGF
Sbjct: 496  FDEHSVLDKIVPGYYLRRNRDGFIENSTCINNTASEHFMVERLILDDLVHWALNYKIDGF 555

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMK TMV+AK ALHCLT +KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 556  RFDLMGHIMKRTMVQAKTALHCLTTEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 615

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQ------------PNGHDHGTEA 2072
            L GTQIGSFNDRIRDAILGGSPFG PLQQGFVTGL LQ            PN HDHGTE 
Sbjct: 616  LPGTQIGSFNDRIRDAILGGSPFGDPLQQGFVTGLLLQGAGVVLAVCGQRPNDHDHGTEE 675

Query: 2073 GMKYMLTTSMDHIQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYV 2252
              K ML  SMDHIQIGMAANLQDFVLTN KGEEVKGSEILTYDGTPVAYA+CP ETINYV
Sbjct: 676  NAKSMLAASMDHIQIGMAANLQDFVLTNCKGEEVKGSEILTYDGTPVAYASCPTETINYV 735

Query: 2253 SAHDNETLFDIVSLKTPMDITLAERCRINHLATSVIALSQ 2372
            SAHDNETLFDIVSLKTPMDIT+ ERCRINHLATS+IALSQ
Sbjct: 736  SAHDNETLFDIVSLKTPMDITVTERCRINHLATSIIALSQ 775


>ref|XP_017409403.1| PREDICTED: pullulanase 1, chloroplastic isoform X4 [Vigna angularis]
          Length = 958

 Score = 1154 bits (2985), Expect = 0.0
 Identities = 574/719 (79%), Positives = 615/719 (85%), Gaps = 13/719 (1%)
 Frame = +3

Query: 312  VEQTASISQMQQDSLLYSRGYWVTESVIAWNVEDGNGF-CYLLASKSASLTIANCKIQGE 488
            VEQ+AS+SQMQ +SLLYSR YWVTE +IAWNV+  N F C L+ASK+ASLTI+N +IQGE
Sbjct: 77   VEQSASLSQMQ-NSLLYSRAYWVTEFLIAWNVDVANEFSCQLIASKNASLTISNGQIQGE 135

Query: 489  DLKIKLHEDRAGLPENVVEKFPHIRGYRAFKLPPIPYVKSLLKSQLAVVSYDSDENCRNC 668
            DLK++L EDRAGLP NV++KFPHIRGY+AFKLP    VK LLKSQLAV  YDSD+ CRNC
Sbjct: 136  DLKVELQEDRAGLPANVLQKFPHIRGYKAFKLPSTLDVKPLLKSQLAVAIYDSDDKCRNC 195

Query: 669  TGLQLPGVLDEIFSYSGPLGALFSEDGVSLYLWAPTAQTVNAYIYKHPSGDDPVEIVSLE 848
            TGLQLPGVLDE+FSY+GPLGALFSE+ VSLYLWAPTAQ V AYIYK PSGDDP+EIV LE
Sbjct: 196  TGLQLPGVLDELFSYNGPLGALFSEEAVSLYLWAPTAQAVRAYIYKDPSGDDPIEIVCLE 255

Query: 849  EEHGVWRTKGPKSWEGXXXXXXXXXXHPSTLRVEKCYPNDPYARGLSADGKRTFLLNLDS 1028
            EE+GVWRTKGPKSWE           H STLRVEKCY NDPYARG+S+DG+RTFLLNLDS
Sbjct: 256  EENGVWRTKGPKSWEHCYYVYEVCVYHHSTLRVEKCYANDPYARGVSSDGQRTFLLNLDS 315

Query: 1029 NELKPDGWDNLANEKPVLRSFSDMSIYEMHIRDFSANDLSVQPDFRGGYLAFTLPDSTGV 1208
            +ELKPDGWD+LANEKP +  F+D+SIYEMHIRDFSA DLSVQP FRGGYLAFTL DS GV
Sbjct: 316  DELKPDGWDDLANEKPTIHCFTDISIYEMHIRDFSATDLSVQPAFRGGYLAFTLQDSAGV 375

Query: 1209 LHLKKLSSAGITHVHLLPTFQFAGVDDQKENWKFVDSSILEELPPDSDQQQALITAIQNL 1388
            LHLK+LSSAGITHVHLLPTFQFAGVDDQKENW+FVD+SILE  PPDSDQQQALITAIQN 
Sbjct: 376  LHLKRLSSAGITHVHLLPTFQFAGVDDQKENWRFVDTSILESFPPDSDQQQALITAIQNF 435

Query: 1389 DGYNWGYNPVLWGVPKGSYASNSNGPYRAIEFRKMVQALNHIGLRVVLDTVYNHLQGSGP 1568
            DGYNWGYNPVLWGVPKGSY+SN NGPYR IEFRKMV+ALNH+GLRVVLD VYNHLQGSGP
Sbjct: 436  DGYNWGYNPVLWGVPKGSYSSNPNGPYRTIEFRKMVKALNHMGLRVVLDVVYNHLQGSGP 495

Query: 1569 FDEHSVLDKIVPGYYLRRSADGFIEHSTCMNNTASEHFMVERLILDDLVHWAVNYKIDGF 1748
            FDEHSVLDKIVPGYYLRR+ DGFIE+STC+NNTASEHFMVERLILDDLVHWA+NYKIDGF
Sbjct: 496  FDEHSVLDKIVPGYYLRRNRDGFIENSTCINNTASEHFMVERLILDDLVHWALNYKIDGF 555

Query: 1749 RFDLMGHIMKSTMVKAKNALHCLTKDKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 1928
            RFDLMGHIMK TMV+AK ALHCLT +KDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN
Sbjct: 556  RFDLMGHIMKRTMVQAKTALHCLTTEKDGVDGSSIYIYGEGWDFGEVAKNGRGINASQFN 615

Query: 1929 LCGTQIGSFNDRIRDAILGGSPFGHPLQQGFVTGLFLQ------------PNGHDHGTEA 2072
            L GTQIGSFNDRIRDAILGGSPFG PLQQGFVTGL LQ            PN HDHGTE 
Sbjct: 616  LPGTQIGSFNDRIRDAILGGSPFGDPLQQGFVTGLLLQGAGVVLAVCGQRPNDHDHGTEE 675

Query: 2073 GMKYMLTTSMDHIQIGMAANLQDFVLTNSKGEEVKGSEILTYDGTPVAYATCPIETINYV 2252
              K ML  SMDHIQIGMAANLQDFVLTN KGEEVKGSEILTYDGTPVAYA+CP ETINYV
Sbjct: 676  NAKSMLAASMDHIQIGMAANLQDFVLTNCKGEEVKGSEILTYDGTPVAYASCPTETINYV 735

Query: 2253 SAHDNETLFDIVSLKTPMDITLAERCRINHLATSVIALSQGIPFFHSGDEILRSKSLDR 2429
            SAHDNETLFDIVSLK                         GIPFFH GDEILRSKSLDR
Sbjct: 736  SAHDNETLFDIVSLK-------------------------GIPFFHCGDEILRSKSLDR 769